Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0015923

Physical Location & Seq

Genomic Coordinates Forward (+)

395069 .. 398384

Gene Structure 3316 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1005000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1713 bp

ATGAATTACTTTCCGGACGAGGTAGTAGAGCACATATTCGAGTCGGTGACCTCGCAGAAGGACCGAAACGCGGTGTCGTTGGTGTGCAAATCGTGGTTCAGAATCGAGAGGTTCAGTAGGGAGAGAGTGTTCATCGGGAATTGCTATGCGATTAGTCCGGAGAGAGTGATCGAGAGGTTTCCGGGGCTCAAGTCTCTGACTTTGAAGGGAAAGCCTCACTTCGCCGACTTCAATTTGGTCCCTCATGAGTGGGGCGGCTTTCTGCAACCGTGGATTGAAGCTCTCGCCGATGGCCGGGTTGGCCTGGAGGAGCTCCGCCTCAAGAGGATGGTCGTGTCCGACGAAAGTCTCGAGCTGCTTTCCAGGTTATTCCCCAATTTCAAGTCTTTGGTGCTTGTTAGCTGTGAAGGGTTTACCACCGAAGGCCTTGCTGCTATTGCTGCCAATTGCAGGTTTCTTAAGGAGCTGGATCTGCAGGAAAATGATATTGATGATCATAGAGGCCATTGGCTAAGCTGCTTTCCTGAGAGCACTACCTCATTAGTTTCCCTGAATTTTGCTTGCCTCAAAGGTGAAATTAATCTAGCGGCTCTGGAGAGACTAGTGGCGAGATCTCCAGACCTCAAAGTATTAAGGTTAAACCGTGCAGTGCCTCCTGACACTCTTCTGAAAGTATTGACGCAAGCGCCTCAACTAGTGGATTTGGGAACCGGGTCTTATGTTCTGGATTCTGATTCTGATACCTACAACAAACTCAAGGCAACTATTCTAAAATGTAAATCAATTAAGAGCTTGTCGGGTTTTTTGGAGGTTGGTCCTCGATGTCTGCCCTCTATTTACCCAATATTGGAAAACCTGACGTCTTTGAACCTCAGCTATGCTTCAGGGGTGCATGGCAGTGAGTTGATAGAGTTAATTCGCCAATGTGTGAAACTTCAGCGATTATGGATTCTGGATTGTATTGGAGACAAAGGACTAGGAGTTGTCGCCAAATCTTGTAAAGAATTGCAGGAATTGAGAGTTTTTCCTTCTGATCCATTTGCAGCTGGGCATGCTTCTGTAACAGAAGAAGGCCTTGTTGCCATATCTGCTGGTTGCCCAAAGCTTCATTCGTTGCTCTACTTCTGCCAGCAAATGACTAATGCTGCTCTCATAACCGTGGCCAAGAACTGCCCAAACTTTATACGTTTCAGGTTGTGCATCCTTGACCCCACTAAACCTGACGCTGTCACCATGCAGCCTCTGGATGAAGGTTTTGGGGCAATTGTTCAGGCATGCAAGAAAATTAGGCGGTTGTCGCTCAGTGGCCTTTTGACTGACCAGGTTTTTCTGTACATTGGAATGTATGCTGAGCAGCTTGAGATGCTTTCTATTGCATTTGCCGGGGACAGTGACAAAGGTATGCTTTATGTGTTGAATGGATGCAAGAAGCTCCGGAAGCTTGAGATCAGGGACAGCCCCTTCGGAAACAAGGCACTTCTAAGGGACGTGGGAAAGTATGAAGCAATGCGATCCCTTTGGATGTCGTCCTGCGAAGTTACTCTGGGAGGCTGCAAGGCACTAGCAAAGAAGATGCCAAGGCTGAATGTGGAGATCATAAATGAGAATGATCAGATGGACCTTGATGATGAGCAACGAGTGGAGAAGATGTACCTCTACCGCACCCTAGTAGGGAAGAGAAGAGATACGCCGGAGTTTGTGTGGACTCTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0000822 GO:0001101 GO:0003006 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0007154 GO:0007165 GO:0007275 GO:0008144 GO:0008150 GO:0009555 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009791 GO:0009908 GO:0009987 GO:0010011 GO:0010033 GO:0010035 GO:0010152 GO:0010167 GO:0016020 GO:0019005 GO:0021700 GO:0022414 GO:0022622 GO:0023052 GO:0031090 GO:0031461 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0036094 GO:0042221 GO:0042562 GO:0043167 GO:0043168 GO:0043178 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048229 GO:0048364 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048527 GO:0048528 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071241 GO:0071249 GO:0071310 GO:0071365 GO:0071495 GO:0080022 GO:0090567 GO:0090696 GO:0098588 GO:0098805 GO:0099402 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902170 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box-like LRR_6

Protein Analysis

571

Amino Acids

63.97

Weight (kDa)

7.04

Isoelectric Point (pI)

41.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F-box_5	PF18511	4 - 41	2.5e-16	F-box
Transp_inhibit	PF18791	61 - 107	5.3e-22	Transport inhibitor response 1 protein domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015923)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12820 AT3G26810
fragaria_vesca	FvH4_6g54140
malus_domestica	MD09G1005000.v1.1 MD17G1009000.v1.1
prunus_persica	Prupe.3G311800_v2.0.a1
pyrus_communis	pycom111g00370
rosa_chinensis	RchiOBHm_Chr2g0176311
rosa_laevigata	RLG00000022402
rosa_multiflora	Rmu_sc0000405.1_g000027
rosa_roxburghii	Rroxscaffold_2G00076580
rosa_rugosa	Rorug02G0593100
rosa_samantha	Rh2CG645600 Rh2DG696400
rosa_wichuraiana	Rw2G055070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	863
Acc36I	ACCTGC	1 cut(s)	441
AccII	CGCG	1 cut(s)	71
AccIII	TCCGGA	3 cut(s)	13, 157, 1434
AciI	CCGC	6 cut(s)	71, 255, 316, 587, 1291, 1660
AclWI	GGATC	3 cut(s)	477, 1028, 1506
AcoI	YGGCCR	2 cut(s)	292, 1161
AcsI	RAATTY	1 cut(s)	553
AcuI	CTGAAG	2 cut(s)	867, 920
AcyI	GRCGYC	1 cut(s)	860
AfaI	GTAC	2 cut(s)	1334, 1652
AfiI	CCNNNNNNNGG	5 cut(s)	70, 294, 450, 847, 1671
AflII	CTTAAG	1 cut(s)	458
AgsI	TTSAA	6 cut(s)	205, 232, 278, 382, 868, 1417
AhlI	ACTAGT	2 cut(s)	601, 694
AjiI	CACGTC	1 cut(s)	1489
AjnI	CCWGG	3 cut(s)	303, 362, 1320
AjuI	GAANNNNNNNTTGG	2 cut(s)	1093, 1125
AloI	GAACNNNNNNTCC	2 cut(s)	113, 145
Alw21I	GWGCWC	3 cut(s)	33, 315, 533
Alw26I	GTCTC	4 cut(s)	198, 353, 592, 960
AlwI	GGATC	3 cut(s)	477, 1028, 1506
AlwNI	CAGNNNCTG	1 cut(s)	1243
Ama87I	CYCGRG	1 cut(s)	350
Aor13HI	TCCGGA	3 cut(s)	13, 157, 1434
AoxI	GGCC	7 cut(s)	292, 301, 424, 502, 1072, 1161, 1306
ApeKI	GCWGC	9 cut(s)	355, 431, 440, 516, 1043, 1145, 1237, 1354, 1551
ApoI	RAATTY	1 cut(s)	553
ArsI	GACNNNNNNTTYG	2 cut(s)	1445, 1477
AseI	ATTAAT	1 cut(s)	579
AspLEI	GCGC	1 cut(s)	688
AspS9I	GGNCC	4 cut(s)	61, 238, 815, 1618
AsuC2I	CCSGG	4 cut(s)	183, 296, 712, 1384
AsuHPI	GGTGA	3 cut(s)	58, 584, 1222
AvaI	CYCGRG	1 cut(s)	350
AvaII	GGWCC	4 cut(s)	61, 238, 815, 1618
BalI	TGGCCA	1 cut(s)	1163
BanII	GRGCYC	2 cut(s)	189, 315
Bbv12I	GWGCWC	3 cut(s)	33, 315, 533
BbvCI	CCTCAGC	1 cut(s)	872
BbvI	GCAGC	9 cut(s)	342, 418, 427, 503, 1055, 1132, 1249, 1366, 1538
BccI	CCATC	3 cut(s)	284, 322, 1609
BcgI	CGANNNNNNTGC	2 cut(s)	410, 444
BciT130I	CCWGG	3 cut(s)	305, 364, 1322
BclI	TGATCA	2 cut(s)	493, 1609
BcnI	CCSGG	4 cut(s)	183, 296, 712, 1384
BcoDI	GTCTC	4 cut(s)	198, 353, 592, 960
BcuI	ACTAGT	2 cut(s)	601, 694
BfaI	CTAG	6 cut(s)	584, 602, 695, 977, 1562, 1667
BfmI	CTRYAG	2 cut(s)	473, 1709
BfoI	RGCGCY	1 cut(s)	689
BfrI	CTTAAG	1 cut(s)	458
BfuAI	ACCTGC	1 cut(s)	441
BglI	GCCNNNNNGGC	1 cut(s)	300
BglII	AGATCT	1 cut(s)	611
BlpI	GCTNAGC	2 cut(s)	512, 1350
Bme1390I	CCNGG	7 cut(s)	183, 296, 305, 364, 712, 1322, 1384
Bme18I	GGWCC	4 cut(s)	61, 238, 815, 1618
BmeT110I	CYCGRG	1 cut(s)	350
BmgBI	CACGTC	1 cut(s)	1489
BmgT120I	GGNCC	4 cut(s)	61, 238, 815, 1618
BmiI	GGNNCC	2 cut(s)	240, 709
BmrFI	CCNGG	7 cut(s)	183, 296, 305, 364, 712, 1322, 1384
BmsI	GCATC	4 cut(s)	1209, 1353, 1412, 1563
BoxI	GACNNNNGTC	1 cut(s)	345
BpmI	CTGGAG	3 cut(s)	326, 600, 614
Bpu10I	CCTNAGC	1 cut(s)	872
Bpu1102I	GCTNAGC	2 cut(s)	512, 1350
BpuEI	CTTGAG	5 cut(s)	173, 305, 740, 1379, 1463
BpuMI	CCSGG	4 cut(s)	183, 296, 712, 1384
BsaHI	GRCGYC	1 cut(s)	860
BsaJI	CCNNGG	5 cut(s)	182, 269, 1158, 1383, 1577
BsaWI	WCCGGW	3 cut(s)	13, 157, 1434
BsaXI	ACNNNNNCTCC	5 cut(s)	113, 143, 1634, 1664, 1685
Bsc4I	CCNNNNNNNGG	5 cut(s)	70, 294, 450, 847, 1671
Bse3DI	GCAATG	1 cut(s)	1512
BseAI	TCCGGA	3 cut(s)	13, 157, 1434
BseBI	CCWGG	3 cut(s)	305, 364, 1322
BseDI	CCNNGG	5 cut(s)	182, 269, 1158, 1383, 1577
BseGI	GGATG	5 cut(s)	333, 1200, 1252, 1427, 1527
BseLI	CCNNNNNNNGG	5 cut(s)	70, 294, 450, 847, 1671
BseMI	GCAATG	1 cut(s)	1512
BseMII	CTCAG	4 cut(s)	516, 886, 1315, 1341
BseRI	GAGGAG	1 cut(s)	323
BseXI	GCAGC	9 cut(s)	342, 418, 427, 503, 1055, 1132, 1249, 1366, 1538
BseYI	CCCAGC	1 cut(s)	1046
BsgI	GTGCAG	1 cut(s)	666
Bsh1236I	CGCG	1 cut(s)	71
BshFI	GGCC	7 cut(s)	294, 303, 426, 504, 1074, 1163, 1308
BsiHKAI	GWGCWC	3 cut(s)	33, 315, 533
BsiHKCI	CYCGRG	1 cut(s)	350
BsiSI	CCGG	8 cut(s)	14, 158, 182, 295, 711, 1383, 1435, 1691
BslFI	GGGAC	4 cut(s)	224, 1400, 1466, 1499
BslI	CCNNNNNNNGG	5 cut(s)	70, 294, 450, 847, 1671
BsmAI	GTCTC	4 cut(s)	198, 353, 592, 960
BsmFI	GGGAC	4 cut(s)	224, 1400, 1466, 1499
BsnI	GGCC	7 cut(s)	294, 303, 426, 504, 1074, 1163, 1308
BsoBI	CYCGRG	1 cut(s)	350
Bsp1286I	GDGCHC	4 cut(s)	33, 189, 315, 533
Bsp13I	TCCGGA	3 cut(s)	13, 157, 1434
Bsp1407I	TGTACA	1 cut(s)	1332
Bsp143I	GATC	9 cut(s)	168, 469, 493, 611, 1033, 1446, 1511, 1593, 1609
Bsp1720I	GCTNAGC	2 cut(s)	512, 1350
BspACI	CCGC	6 cut(s)	71, 255, 316, 587, 1291, 1660
BspANI	GGCC	7 cut(s)	294, 303, 426, 504, 1074, 1163, 1308
BspCNI	CTCAG	4 cut(s)	517, 885, 1314, 1342
BspEI	TCCGGA	3 cut(s)	13, 157, 1434
BspFNI	CGCG	1 cut(s)	71
BspHI	TCATGA	1 cut(s)	244
BspLI	GGNNCC	2 cut(s)	240, 709
BspMAI	CTGCAG	1 cut(s)	477
BspMI	ACCTGC	1 cut(s)	441
BspPI	GGATC	3 cut(s)	477, 1028, 1506
BspTI	CTTAAG	1 cut(s)	458
BsrDI	GCAATG	1 cut(s)	1512
BsrGI	TGTACA	1 cut(s)	1332
BssECI	CCNNGG	5 cut(s)	182, 269, 1158, 1383, 1577
BssMI	GATC	9 cut(s)	168, 469, 493, 611, 1033, 1446, 1511, 1593, 1609
BssNI	GRCGYC	1 cut(s)	860
BssT1I	CCWWGG	1 cut(s)	1577
Bst2UI	CCWGG	3 cut(s)	305, 364, 1322
Bst4CI	ACNGT	4 cut(s)	270, 644, 1159, 1391
Bst6I	CTCTTC	3 cut(s)	669, 1670, 1675
BstACI	GRCGYC	1 cut(s)	860
BstAFI	CTTAAG	1 cut(s)	458
BstAUI	TGTACA	1 cut(s)	1332
BstC8I	GCNNGC	5 cut(s)	562, 684, 1053, 1130, 1276
BstDEI	CTNAG	6 cut(s)	512, 525, 872, 1301, 1350, 1481
BstDSI	CCRYGG	2 cut(s)	269, 1158
BstEII	GGTNACC	1 cut(s)	46
BstF5I	GGATG	5 cut(s)	333, 1200, 1252, 1427, 1527
BstFNI	CGCG	1 cut(s)	71
BstH2I	RGCGCY	1 cut(s)	689
BstHHI	GCGC	1 cut(s)	688
BstKTI	GATC	9 cut(s)	171, 472, 496, 614, 1036, 1449, 1514, 1596, 1612
BstMAI	GTCTC	4 cut(s)	198, 353, 592, 960
BstMBI	GATC	9 cut(s)	168, 469, 493, 611, 1033, 1446, 1511, 1593, 1609
BstMWI	GCNNNNNNNGC	9 cut(s)	300, 437, 440, 472, 1052, 1088, 1297, 1363, 1438
BstNI	CCWGG	3 cut(s)	305, 364, 1322
BstNSI	RCATGY	2 cut(s)	1055, 1278
BstPAI	GACNNNNGTC	1 cut(s)	345
BstPI	GGTNACC	1 cut(s)	46
BstSCI	CCNGG	7 cut(s)	181, 294, 303, 362, 710, 1320, 1382
BstSFI	CTRYAG	2 cut(s)	473, 1709
BstUI	CGCG	1 cut(s)	71
BstV1I	GCAGC	9 cut(s)	342, 418, 427, 503, 1055, 1132, 1249, 1366, 1538
BstX2I	RGATCY	2 cut(s)	469, 611
BstYI	RGATCY	2 cut(s)	469, 611
BsuRI	GGCC	7 cut(s)	294, 303, 426, 504, 1074, 1163, 1308
BtgI	CCRYGG	2 cut(s)	269, 1158
BtrI	CACGTC	1 cut(s)	1489
BtsCI	GGATG	5 cut(s)	333, 1200, 1252, 1427, 1527
BtsI	GCAGTG	2 cut(s)	654, 904
BtsIMutI	CAGTG	4 cut(s)	654, 904, 1309, 1396
BveI	ACCTGC	1 cut(s)	441
Cac8I	GCNNGC	5 cut(s)	562, 684, 1053, 1130, 1276
CaiI	CAGNNNCTG	1 cut(s)	1243
CciI	TCATGA	1 cut(s)	244
CfoI	GCGC	1 cut(s)	688
Cfr13I	GGNCC	4 cut(s)	61, 238, 815, 1618
CseI	GACGC	2 cut(s)	688, 1232
CsiI	ACCWGGT	1 cut(s)	1320
Csp6I	GTAC	2 cut(s)	1333, 1651
CviAII	CATG	5 cut(s)	245, 893, 1052, 1234, 1275
CviQI	GTAC	2 cut(s)	1333, 1651
DdeI	CTNAG	6 cut(s)	512, 525, 872, 1301, 1350, 1481
DpnI	GATC	9 cut(s)	170, 471, 495, 613, 1035, 1448, 1513, 1595, 1611
DpnII	GATC	9 cut(s)	168, 469, 493, 611, 1033, 1446, 1511, 1593, 1609
EaeI	YGGCCR	2 cut(s)	292, 1161
Eam1104I	CTCTTC	3 cut(s)	669, 1670, 1675
EarI	CTCTTC	3 cut(s)	669, 1670, 1675
EciI	GGCGGA	1 cut(s)	305
Ecl136II	GAGCTC	1 cut(s)	313
Eco130I	CCWWGG	1 cut(s)	1577
Eco147I	AGGCCT	2 cut(s)	426, 1074
Eco24I	GRGCYC	2 cut(s)	189, 315
Eco47I	GGWCC	4 cut(s)	61, 238, 815, 1618
Eco53kI	GAGCTC	1 cut(s)	313
Eco57I	CTGAAG	2 cut(s)	867, 920
Eco88I	CYCGRG	1 cut(s)	350
Eco91I	GGTNACC	1 cut(s)	46
EcoICRI	GAGCTC	1 cut(s)	313
EcoO65I	GGTNACC	1 cut(s)	46
EcoRII	CCWGG	3 cut(s)	303, 362, 1320
EcoT14I	CCWWGG	1 cut(s)	1577
EcoT38I	GRGCYC	2 cut(s)	189, 315
ErhI	CCWWGG	1 cut(s)	1577
FaeI	CATG	5 cut(s)	248, 896, 1055, 1237, 1278
FalI	AAGNNNNNCTT	6 cut(s)	203, 235, 1059, 1091, 1389, 1421
FaqI	GGGAC	4 cut(s)	224, 1400, 1466, 1499
FatI	CATG	5 cut(s)	244, 892, 1051, 1233, 1274
FbaI	TGATCA	2 cut(s)	493, 1609
FokI	GGATG	5 cut(s)	340, 1187, 1259, 1434, 1534
FriOI	GRGCYC	2 cut(s)	189, 315
FspBI	CTAG	6 cut(s)	584, 602, 695, 977, 1562, 1667
GlaI	GCGC	1 cut(s)	687
GsaI	CCCAGC	1 cut(s)	1050
GsuI	CTGGAG	3 cut(s)	326, 600, 614
HaeII	RGCGCY	1 cut(s)	689
HaeIII	GGCC	7 cut(s)	294, 303, 426, 504, 1074, 1163, 1308
HapII	CCGG	8 cut(s)	14, 158, 182, 295, 711, 1383, 1435, 1691
HgaI	GACGC	2 cut(s)	688, 1232
HhaI	GCGC	1 cut(s)	688
Hin1I	GRCGYC	1 cut(s)	860
Hin1II	CATG	5 cut(s)	248, 896, 1055, 1237, 1278
Hin6I	GCGC	1 cut(s)	686
HinP1I	GCGC	1 cut(s)	686
HindIII	AAGCTT	2 cut(s)	1103, 1439
HinfI	GANTC	6 cut(s)	41, 102, 728, 734, 949, 1705
HpaII	CCGG	8 cut(s)	14, 158, 182, 295, 711, 1383, 1435, 1691
HphI	GGTGA	3 cut(s)	58, 584, 1222
Hpy166II	GTNNAC	2 cut(s)	414, 1704
Hpy188I	TCNGA	9 cut(s)	101, 198, 340, 669, 733, 739, 1033, 1466, 1614
Hpy8I	GTNNAC	2 cut(s)	414, 1704
Hpy99I	CGWCG	1 cut(s)	344
HpyAV	CCTTC	8 cut(s)	52, 199, 401, 416, 1038, 1064, 1244, 1471
HpyCH4III	ACNGT	4 cut(s)	270, 644, 1159, 1391
HpyCH4IV	ACGT	3 cut(s)	860, 1186, 1488
HpyF10VI	GCNNNNNNNGC	9 cut(s)	300, 437, 440, 472, 1052, 1088, 1297, 1363, 1438
HpyF3I	CTNAG	6 cut(s)	512, 525, 872, 1301, 1350, 1481
HpySE526I	ACGT	3 cut(s)	860, 1186, 1488
Hsp92I	GRCGYC	1 cut(s)	860
Hsp92II	CATG	5 cut(s)	248, 896, 1055, 1237, 1278
HspAI	GCGC	1 cut(s)	686
Kpn2I	TCCGGA	3 cut(s)	13, 157, 1434
Ksp22I	TGATCA	2 cut(s)	493, 1609
Kzo9I	GATC	9 cut(s)	168, 469, 493, 611, 1033, 1446, 1511, 1593, 1609
LmnI	GCTCC	4 cut(s)	310, 318, 463, 1437
Lsp1109I	GCAGC	9 cut(s)	342, 418, 427, 503, 1055, 1132, 1249, 1366, 1538
LweI	GCATC	4 cut(s)	1209, 1353, 1412, 1563
MabI	ACCWGGT	1 cut(s)	1320
MaeI	CTAG	6 cut(s)	584, 602, 695, 977, 1562, 1667
MaeII	ACGT	3 cut(s)	860, 1186, 1488
MaeIII	GTNAC	5 cut(s)	46, 1060, 1228, 1391, 1537
MalI	GATC	9 cut(s)	170, 471, 495, 613, 1035, 1448, 1513, 1595, 1611
MboI	GATC	9 cut(s)	168, 469, 493, 611, 1033, 1446, 1511, 1593, 1609
MboII	GAAGA	6 cut(s)	656, 1079, 1582, 1657, 1687, 1692
MfeI	CAATTG	2 cut(s)	445, 1263
MflI	RGATCY	2 cut(s)	469, 611
MhlI	GDGCHC	4 cut(s)	33, 189, 315, 533
MlsI	TGGCCA	1 cut(s)	1163
MluNI	TGGCCA	1 cut(s)	1163
MlyI	GAGTC	2 cut(s)	50, 1699
MmeI	TCCRAC	1 cut(s)	363
Mox20I	TGGCCA	1 cut(s)	1163
MroI	TCCGGA	3 cut(s)	13, 157, 1434
MscI	TGGCCA	1 cut(s)	1163
MseI	TTAA	6 cut(s)	459, 579, 632, 638, 786, 914
MslI	CAYNNNNRTG	3 cut(s)	897, 1157, 1340
Msp20I	TGGCCA	1 cut(s)	1163
MspA1I	CMGCKG	1 cut(s)	1046
MspCI	CTTAAG	1 cut(s)	458
MspI	CCGG	8 cut(s)	14, 158, 182, 295, 711, 1383, 1435, 1691
MspR9I	CCNGG	7 cut(s)	183, 296, 305, 364, 712, 1322, 1384
MunI	CAATTG	2 cut(s)	445, 1263
MvaI	CCWGG	3 cut(s)	305, 364, 1322
MvnI	CGCG	1 cut(s)	71
MwoI	GCNNNNNNNGC	9 cut(s)	300, 437, 440, 472, 1052, 1088, 1297, 1363, 1438
NciI	CCSGG	4 cut(s)	183, 296, 712, 1384
NdeII	GATC	9 cut(s)	168, 469, 493, 611, 1033, 1446, 1511, 1593, 1609
NlaIII	CATG	5 cut(s)	248, 896, 1055, 1237, 1278
NlaIV	GGNNCC	2 cut(s)	240, 709
NmuCI	GTSAC	3 cut(s)	46, 1228, 1391
NspI	RCATGY	2 cut(s)	1055, 1278
PaeI	GCATGC	2 cut(s)	1055, 1278
PaeR7I	CTCGAG	1 cut(s)	350
PagI	TCATGA	1 cut(s)	244
PceI	AGGCCT	2 cut(s)	426, 1074
PcsI	WCGNNNNNNNCGW	2 cut(s)	339, 348
PfeI	GAWTC	4 cut(s)	102, 728, 734, 949
PflFI	GACNNNGTC	1 cut(s)	1226
PleI	GAGTC	2 cut(s)	49, 1699
PpsI	GAGTC	2 cut(s)	49, 1699
PshAI	GACNNNNGTC	1 cut(s)	345
PshBI	ATTAAT	1 cut(s)	579
Psp124BI	GAGCTC	1 cut(s)	315
Psp6I	CCWGG	3 cut(s)	303, 362, 1320
PspEI	GGTNACC	1 cut(s)	46
PspFI	CCCAGC	1 cut(s)	1046
PspGI	CCWGG	3 cut(s)	303, 362, 1320
PspN4I	GGNNCC	2 cut(s)	240, 709
PspPI	GGNCC	4 cut(s)	61, 238, 815, 1618
PstI	CTGCAG	1 cut(s)	477
PstNI	CAGNNNCTG	1 cut(s)	1243
PsuI	RGATCY	2 cut(s)	469, 611
PsyI	GACNNNGTC	1 cut(s)	1226
PvuII	CAGCTG	1 cut(s)	1046
RsaI	GTAC	2 cut(s)	1334, 1652
RsaNI	GTAC	2 cut(s)	1333, 1651
RseI	CAYNNNNRTG	3 cut(s)	897, 1157, 1340
SacI	GAGCTC	1 cut(s)	315
SaqAI	TTAA	6 cut(s)	459, 579, 632, 638, 786, 914
Sau3AI	GATC	9 cut(s)	168, 469, 493, 611, 1033, 1446, 1511, 1593, 1609
Sau96I	GGNCC	4 cut(s)	61, 238, 815, 1618
SchI	GAGTC	2 cut(s)	50, 1699
ScrFI	CCNGG	7 cut(s)	183, 296, 305, 364, 712, 1322, 1384
SduI	GDGCHC	4 cut(s)	33, 189, 315, 533
SexAI	ACCWGGT	1 cut(s)	1320
SfaNI	GCATC	4 cut(s)	1209, 1353, 1412, 1563
SfcI	CTRYAG	2 cut(s)	473, 1709
SfiI	GGCCNNNNNGGCC	1 cut(s)	300
Sfr274I	CTCGAG	1 cut(s)	350
SinI	GGWCC	4 cut(s)	61, 238, 815, 1618
SlaI	CTCGAG	1 cut(s)	350
SmiMI	CAYNNNNRTG	3 cut(s)	897, 1157, 1340
SmlI	CTYRAG	7 cut(s)	188, 320, 350, 458, 755, 1358, 1442
SmoI	CTYRAG	7 cut(s)	188, 320, 350, 458, 755, 1358, 1442
SpeI	ACTAGT	2 cut(s)	601, 694
SphI	GCATGC	2 cut(s)	1055, 1278
SseBI	AGGCCT	2 cut(s)	426, 1074
SsiI	CCGC	6 cut(s)	71, 255, 316, 587, 1291, 1660
SspI	AATATT	1 cut(s)	846
SspMI	CTAG	6 cut(s)	584, 602, 695, 977, 1562, 1667
SstI	GAGCTC	1 cut(s)	315
StuI	AGGCCT	2 cut(s)	426, 1074
StyD4I	CCNGG	7 cut(s)	181, 294, 303, 362, 710, 1320, 1382
StyI	CCWWGG	1 cut(s)	1577
TaaI	ACNGT	4 cut(s)	270, 644, 1159, 1391
TaiI	ACGT	3 cut(s)	863, 1189, 1491
TaqI	TCGA	5 cut(s)	39, 105, 171, 351, 820
TaqII	GACCGA	1 cut(s)	78
TatI	WGTACW	1 cut(s)	1332
TauI	GCSGC	2 cut(s)	258, 590
TfiI	GAWTC	4 cut(s)	102, 728, 734, 949
Tru1I	TTAA	6 cut(s)	459, 579, 632, 638, 786, 914
Tru9I	TTAA	6 cut(s)	459, 579, 632, 638, 786, 914
TscAI	CASTG	4 cut(s)	654, 904, 1309, 1396
TseFI	GTSAC	3 cut(s)	46, 1228, 1391
TseI	GCWGC	9 cut(s)	355, 431, 440, 516, 1043, 1145, 1237, 1354, 1551
Tsp45I	GTSAC	3 cut(s)	46, 1228, 1391
TspDTI	ATGAA	5 cut(s)	17, 121, 1097, 1263, 1515
TspRI	CASTG	4 cut(s)	654, 904, 1309, 1396
Tth111I	GACNNNGTC	1 cut(s)	1226
Vha464I	CTTAAG	1 cut(s)	458
VpaK11BI	GGWCC	4 cut(s)	61, 238, 815, 1618
VspI	ATTAAT	1 cut(s)	579
XapI	RAATTY	1 cut(s)	553
XceI	RCATGY	2 cut(s)	1055, 1278
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1240
XhoI	CTCGAG	1 cut(s)	350
XspI	CTAG	6 cut(s)	584, 602, 695, 977, 1562, 1667
ZraI	GACGTC	1 cut(s)	861

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.