Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0012460

Physical Location & Seq

Genomic Coordinates Reverse (-)

6698820 .. 6700984

Gene Structure 2165 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1091400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1143 bp

ATGCAGACCCGGTTCACAGCCCTAGCGGCTCGGATCGTAAAATCGACCGCCCGGTCCTTCTCCTCCTCCACCCAAACCCCGCCCTCAACCGCCACTTCCTCCTCCCAATTCCCCCAAACTCTGGCAGGCCTGCGGGCCCGCCTTGCCGCCGAGTCTCCGATACTGACGGACTTCGTGGAAGGCGAAGGCCCGTATTCTGTTGAAGTCGGAACCAAGAAGAACCCTCTCCCGAAACCGAAATGGATGAAGGAGTCGATCCCCGGCGGCCAAAAGTACACTCAGATCAAGAAAAAGCTCAGGGAATTGAAGCTTCACACGGTGTGCGAGGAGGCCAAGTGCCCCAACCTCGGCGAGTGCTGGTCCGGCGGCGAGACCGGCACCGCCACTGCCACGATTATGATTCTTGGTGATACGTGTACGCGCGGTTGCAGGTTTTGCAATGTGAAGACTTCCAGGACTCCGCCACCGCCGGACCCTAATGAACCGACGAATGTGGCGGAGGCGATTGCGTCTTGGGGTTTGGATTATGTGGTGATTACTAGCGTGGACCGGGACGATTTGCCCGATCAAGGGAGCGGACATTTTGCAGAGACCGTGCAAAAGTTGAAGGTGCTTAAACCCAGCATGCTCATTGAGGCTTTGGTTCCTGATTTTCGGGGAGACTCGAGCTGCGTAGAGAAAGTTGCAACATCCGGATTGGATGTCCTTGCTCACAATATTGAGACAGTTGAAGAGCTTCAAAGGGCAGTGCGGGATCACCGTGCCAATTTCAAGCAATCTTTAGATGTTTTAATGATGGCCAAAGACCATGCTCCTGCAGGAACACTTACAAAGACTTCAATAATGTTAGGCTGCGGGGAGACACCTGATCAGGTTGTGAGGACAATGGAGAAGGTGAGAGCAGCAGGTGTTGATGTTATGACATTTGGTCAATACATGCGACCTTCGAAGCGCCACATGCCTGTGTCTGAATACATTACTCCTGAGGCCTTTGAGAGATACCGAGCTCTTGGCATGGAAATGGGATTTCGATATGTGGCATCTGGTCCCATGGTAAGGTCGTCATACAAAGCTGGTGAATACTACATCAAATCTATGATAGAATCAGACCGGGCTTCATCTTCGCATGTTTCTGCCTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001505 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006464 GO:0006520 GO:0006544 GO:0006546 GO:0006629 GO:0006631 GO:0006633 GO:0006732 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0008610 GO:0009056 GO:0009058 GO:0009063 GO:0009069 GO:0009071 GO:0009106 GO:0009107 GO:0009108 GO:0009249 GO:0009987 GO:0010467 GO:0016053 GO:0016054 GO:0016740 GO:0016782 GO:0016783 GO:0016992 GO:0017144 GO:0018065 GO:0018130 GO:0018193 GO:0018205 GO:0019538 GO:0019752 GO:0031974 GO:0032787 GO:0036211 GO:0042133 GO:0042135 GO:0042737 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044272 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046395 GO:0046483 GO:0051186 GO:0051188 GO:0051604 GO:0065007 GO:0065008 GO:0070013 GO:0070283 GO:0071704 GO:0072330 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1901605 GO:1901606

KEGG Pathways

Metabolic & Signaling

ko00785 ko01100 map00785 map01100

Pfam Domains

Protein Families

LIAS_N Radical_SAM

Protein Analysis

381

Amino Acids

41.56

Weight (kDa)

8.51

Isoelectric Point (pI)

37.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LIAS_N	PF16881	45 - 113	1.4e-14	N-terminal domain of lipoyl synthase of Radical_SAM family
Radical_SAM	PF04055	133 - 298	1.1e-20	Radical SAM superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012460)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20860 AT2G20860 AT2G20860
fragaria_vesca	FvH4_6g44960
malus_domestica	MD09G1091400.v1.1 MD17G1081300.v1.1
prunus_persica	Prupe.3G233600_v2.0.a1
pyrus_communis	pycom09g01700 pycom17g07960
rosa_chinensis	RchiOBHm_Chr2g0162201
rosa_laevigata	RLG00000021327
rosa_multiflora	Rmu_sc0004020.1_g000029
rosa_roxburghii	Rroxscaffold_2G00088250
rosa_rugosa	Rorug02G0495200
rosa_samantha	Rh2AG561600 Rh2BG574700 Rh2CG544900 Rh2DG584700
rosa_wichuraiana	Rw2G046510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	896
AasI	GACNNNNNNGTC	1 cut(s)	52
Acc36I	ACCTGC	2 cut(s)	420, 896
AccB1I	GGYRCC	1 cut(s)	377
AccB7I	CCANNNNNTGG	1 cut(s)	121
AccBSI	CCGCTC	1 cut(s)	576
AccII	CGCG	2 cut(s)	421, 423
AccIII	TCCGGA	1 cut(s)	692
AclWI	GGATC	3 cut(s)	41, 250, 762
AcoI	YGGCCR	2 cut(s)	265, 798
AdeI	CACNNNGTG	2 cut(s)	319, 321
AfaI	GTAC	2 cut(s)	275, 418
AfiI	CCNNNNNNNGG	5 cut(s)	121, 347, 569, 570, 1056
AflIII	ACRYGT	1 cut(s)	413
AgsI	TTSAA	7 cut(s)	203, 307, 607, 731, 740, 772, 840
AhdI	GACNNNNNGTC	1 cut(s)	927
AjnI	CCWGG	1 cut(s)	452
AluBI	AGCT	6 cut(s)	295, 310, 669, 736, 1007, 1073
AluI	AGCT	6 cut(s)	295, 310, 669, 736, 1007, 1073
Alw21I	GWGCWC	1 cut(s)	1009
Alw26I	GTCTC	6 cut(s)	159, 365, 584, 654, 716, 854
AlwI	GGATC	3 cut(s)	41, 250, 762
Ama87I	CYCGRG	1 cut(s)	664
Aor13HI	TCCGGA	1 cut(s)	692
AoxI	GGCC	7 cut(s)	127, 135, 187, 265, 330, 798, 987
ApaI	GGGCCC	1 cut(s)	139
ApeKI	GCWGC	3 cut(s)	669, 852, 902
Asp700I	GAANNNNTTC	1 cut(s)	735
AspLEI	GCGC	2 cut(s)	423, 954
AspS9I	GGNCC	8 cut(s)	54, 135, 136, 188, 360, 472, 547, 1046
AsuC2I	CCSGG	5 cut(s)	10, 52, 261, 551, 1112
AsuHPI	GGTGA	5 cut(s)	419, 544, 749, 907, 1088
AsuII	TTCGAA	1 cut(s)	947
AvaI	CYCGRG	1 cut(s)	664
AvaII	GGWCC	5 cut(s)	54, 360, 472, 547, 1046
AxyI	CCTNAGG	1 cut(s)	984
BaeGI	GKGCMC	2 cut(s)	139, 341
BalI	TGGCCA	1 cut(s)	800
BanI	GGYRCC	1 cut(s)	377
BanII	GRGCYC	2 cut(s)	139, 1009
BauI	CACGAG	1 cut(s)	1138
BbsI	GAAGAC	1 cut(s)	452
Bbv12I	GWGCWC	1 cut(s)	1009
BbvI	GCAGC	3 cut(s)	656, 839, 914
BccI	CCATC	1 cut(s)	790
BciT130I	CCWGG	1 cut(s)	454
BclI	TGATCA	1 cut(s)	868
BcnI	CCSGG	5 cut(s)	10, 52, 261, 551, 1112
BcoDI	GTCTC	6 cut(s)	159, 365, 584, 654, 716, 854
BfaI	CTAG	2 cut(s)	23, 540
BfmI	CTRYAG	1 cut(s)	816
BfoI	RGCGCY	1 cut(s)	955
BfuAI	ACCTGC	2 cut(s)	420, 896
BglI	GCCNNNNNGGC	1 cut(s)	26
BisI	GCNGC	7 cut(s)	27, 147, 265, 367, 670, 853, 903
BlsI	GCNGC	7 cut(s)	28, 148, 266, 368, 671, 854, 904
Bme1390I	CCNGG	6 cut(s)	10, 52, 261, 454, 551, 1112
Bme18I	GGWCC	5 cut(s)	54, 360, 472, 547, 1046
BmeRI	GACNNNNNGTC	1 cut(s)	927
BmeT110I	CYCGRG	1 cut(s)	664
BmgT120I	GGNCC	8 cut(s)	54, 135, 136, 188, 360, 472, 547, 1046
BmiI	GGNNCC	6 cut(s)	137, 211, 379, 474, 645, 1048
BmrFI	CCNGG	6 cut(s)	10, 52, 261, 454, 551, 1112
BmsI	GCATC	1 cut(s)	1049
BpiI	GAAGAC	1 cut(s)	452
Bpu10I	CCTNAGC	1 cut(s)	296
Bpu14I	TTCGAA	1 cut(s)	947
BpuMI	CCSGG	5 cut(s)	10, 52, 261, 551, 1112
BsaAI	YACGTR	1 cut(s)	414
BsaI	GGTCTC	2 cut(s)	365, 584
BsaJI	CCNNGG	3 cut(s)	259, 346, 1050
BsaWI	WCCGGW	1 cut(s)	692
BsaXI	ACNNNNNCTCC	2 cut(s)	320, 350
Bsc4I	CCNNNNNNNGG	5 cut(s)	121, 347, 569, 570, 1056
Bse118I	RCCGGY	1 cut(s)	374
Bse21I	CCTNAGG	1 cut(s)	984
Bse3DI	GCAATG	1 cut(s)	445
BseAI	TCCGGA	1 cut(s)	692
BseBI	CCWGG	1 cut(s)	454
BseDI	CCNNGG	3 cut(s)	259, 346, 1050
BseGI	GGATG	3 cut(s)	249, 689, 706
BseLI	CCNNNNNNNGG	5 cut(s)	121, 347, 569, 570, 1056
BseMI	GCAATG	1 cut(s)	445
BseMII	CTCAG	3 cut(s)	293, 310, 975
BseRI	GAGGAG	4 cut(s)	52, 55, 91, 341
BseSI	GKGCMC	2 cut(s)	139, 341
BseXI	GCAGC	3 cut(s)	656, 839, 914
BseYI	CCCAGC	1 cut(s)	620
Bsh1236I	CGCG	2 cut(s)	421, 423
Bsh1285I	CGRYCG	1 cut(s)	48
BshFI	GGCC	7 cut(s)	129, 137, 189, 267, 332, 800, 989
BshNI	GGYRCC	1 cut(s)	377
BsiEI	CGRYCG	1 cut(s)	48
BsiHKAI	GWGCWC	1 cut(s)	1009
BsiHKCI	CYCGRG	1 cut(s)	664
BsiSI	CCGG	9 cut(s)	10, 52, 261, 363, 375, 470, 550, 693, 1111
BslFI	GGGAC	2 cut(s)	566, 1032
BslI	CCNNNNNNNGG	5 cut(s)	121, 347, 569, 570, 1056
BsmAI	GTCTC	6 cut(s)	159, 365, 584, 654, 716, 854
BsmFI	GGGAC	2 cut(s)	566, 1032
BsnI	GGCC	7 cut(s)	129, 137, 189, 267, 332, 800, 989
Bso31I	GGTCTC	2 cut(s)	365, 584
BsoBI	CYCGRG	1 cut(s)	664
Bsp119I	TTCGAA	1 cut(s)	947
Bsp120I	GGGCCC	1 cut(s)	135
Bsp1286I	GDGCHC	3 cut(s)	139, 341, 1009
Bsp13I	TCCGGA	1 cut(s)	692
Bsp143I	GATC	6 cut(s)	33, 255, 282, 565, 754, 868
Bsp19I	CCATGG	1 cut(s)	1050
BspANI	GGCC	7 cut(s)	129, 137, 189, 267, 332, 800, 989
BspCNI	CTCAG	3 cut(s)	292, 309, 976
BspEI	TCCGGA	1 cut(s)	692
BspFNI	CGCG	2 cut(s)	421, 423
BspLI	GGNNCC	6 cut(s)	137, 211, 379, 474, 645, 1048
BspMAI	CTGCAG	1 cut(s)	820
BspMI	ACCTGC	2 cut(s)	420, 896
BspPI	GGATC	3 cut(s)	41, 250, 762
BspQI	GCTCTTC	1 cut(s)	726
BspT104I	TTCGAA	1 cut(s)	947
BspT107I	GGYRCC	1 cut(s)	377
BspTNI	GGTCTC	2 cut(s)	365, 584
BsrBI	CCGCTC	1 cut(s)	576
BsrDI	GCAATG	1 cut(s)	445
BsrFI	RCCGGY	1 cut(s)	374
BssAI	RCCGGY	1 cut(s)	374
BssECI	CCNNGG	3 cut(s)	259, 346, 1050
BssMI	GATC	6 cut(s)	33, 255, 282, 565, 754, 868
BssSI	CACGAG	1 cut(s)	1138
BssT1I	CCWWGG	1 cut(s)	1050
Bst2BI	CACGAG	1 cut(s)	1138
Bst2UI	CCWGG	1 cut(s)	454
Bst4CI	ACNGT	4 cut(s)	319, 595, 727, 761
Bst6I	CTCTTC	1 cut(s)	726
BstAPI	GCANNNNNTGC	1 cut(s)	435
BstBAI	YACGTR	1 cut(s)	414
BstBI	TTCGAA	1 cut(s)	947
BstC8I	GCNNGC	5 cut(s)	127, 131, 135, 139, 626
BstDEI	CTNAG	3 cut(s)	279, 296, 984
BstDSI	CCRYGG	1 cut(s)	1050
BstF5I	GGATG	3 cut(s)	249, 689, 706
BstFNI	CGCG	2 cut(s)	421, 423
BstH2I	RGCGCY	1 cut(s)	955
BstHHI	GCGC	2 cut(s)	423, 954
BstKTI	GATC	6 cut(s)	36, 258, 285, 568, 757, 871
BstMAI	GTCTC	6 cut(s)	159, 365, 584, 654, 716, 854
BstMBI	GATC	6 cut(s)	33, 255, 282, 565, 754, 868
BstMCI	CGRYCG	1 cut(s)	48
BstMWI	GCNNNNNNNGC	6 cut(s)	26, 143, 363, 375, 435, 958
BstNI	CCWGG	1 cut(s)	454
BstNSI	RCATGY	4 cut(s)	628, 940, 961, 1130
BstSCI	CCNGG	6 cut(s)	8, 50, 259, 452, 549, 1110
BstSFI	CTRYAG	1 cut(s)	816
BstSLI	GKGCMC	2 cut(s)	139, 341
BstUI	CGCG	2 cut(s)	421, 423
BstV1I	GCAGC	3 cut(s)	656, 839, 914
BstV2I	GAAGAC	1 cut(s)	452
Bsu36I	CCTNAGG	1 cut(s)	984
BsuRI	GGCC	7 cut(s)	129, 137, 189, 267, 332, 800, 989
BtgI	CCRYGG	1 cut(s)	1050
BtsCI	GGATG	3 cut(s)	249, 689, 706
BtsI	GCAGTG	2 cut(s)	384, 753
BtsIMutI	CAGTG	2 cut(s)	384, 753
BveI	ACCTGC	2 cut(s)	420, 896
Cac8I	GCNNGC	5 cut(s)	127, 131, 135, 139, 626
CfoI	GCGC	2 cut(s)	423, 954
Cfr10I	RCCGGY	1 cut(s)	374
Cfr13I	GGNCC	8 cut(s)	54, 135, 136, 188, 360, 472, 547, 1046
CseI	GACGC	1 cut(s)	498
Csp6I	GTAC	2 cut(s)	274, 417
CviAII	CATG	7 cut(s)	625, 809, 937, 958, 1015, 1051, 1127
CviQI	GTAC	2 cut(s)	274, 417
DdeI	CTNAG	3 cut(s)	279, 296, 984
DpnI	GATC	6 cut(s)	35, 257, 284, 567, 756, 870
DpnII	GATC	6 cut(s)	33, 255, 282, 565, 754, 868
DraIII	CACNNNGTG	2 cut(s)	319, 321
DrdI	GACNNNNNNGTC	1 cut(s)	52
DriI	GACNNNNNGTC	1 cut(s)	927
DseDI	GACNNNNNNGTC	1 cut(s)	52
EaeI	YGGCCR	2 cut(s)	265, 798
Eam1104I	CTCTTC	1 cut(s)	726
Eam1105I	GACNNNNNGTC	1 cut(s)	927
EarI	CTCTTC	1 cut(s)	726
EciI	GGCGGA	2 cut(s)	450, 512
Ecl136II	GAGCTC	1 cut(s)	1007
Eco130I	CCWWGG	1 cut(s)	1050
Eco147I	AGGCCT	2 cut(s)	129, 989
Eco24I	GRGCYC	2 cut(s)	139, 1009
Eco31I	GGTCTC	2 cut(s)	365, 584
Eco47I	GGWCC	5 cut(s)	54, 360, 472, 547, 1046
Eco53kI	GAGCTC	1 cut(s)	1007
Eco81I	CCTNAGG	1 cut(s)	984
Eco88I	CYCGRG	1 cut(s)	664
EcoICRI	GAGCTC	1 cut(s)	1007
EcoRII	CCWGG	1 cut(s)	452
EcoT14I	CCWWGG	1 cut(s)	1050
EcoT38I	GRGCYC	2 cut(s)	139, 1009
ErhI	CCWWGG	1 cut(s)	1050
FaeI	CATG	7 cut(s)	628, 812, 940, 961, 1018, 1054, 1130
FaqI	GGGAC	2 cut(s)	566, 1032
FatI	CATG	7 cut(s)	624, 808, 936, 957, 1014, 1050, 1126
FauI	CCCGC	5 cut(s)	87, 126, 146, 744, 848
FbaI	TGATCA	1 cut(s)	868
Fnu4HI	GCNGC	7 cut(s)	27, 147, 265, 367, 670, 853, 903
FokI	GGATG	3 cut(s)	256, 676, 713
FriOI	GRGCYC	2 cut(s)	139, 1009
Fsp4HI	GCNGC	7 cut(s)	27, 147, 265, 367, 670, 853, 903
FspBI	CTAG	2 cut(s)	23, 540
GlaI	GCGC	2 cut(s)	422, 953
GluI	GCNGC	7 cut(s)	27, 147, 265, 367, 670, 853, 903
GsaI	CCCAGC	1 cut(s)	624
HaeII	RGCGCY	1 cut(s)	955
HaeIII	GGCC	7 cut(s)	129, 137, 189, 267, 332, 800, 989
HapII	CCGG	9 cut(s)	10, 52, 261, 363, 375, 470, 550, 693, 1111
HgaI	GACGC	1 cut(s)	498
HhaI	GCGC	2 cut(s)	423, 954
Hin1II	CATG	7 cut(s)	628, 812, 940, 961, 1018, 1054, 1130
Hin6I	GCGC	2 cut(s)	421, 952
HinP1I	GCGC	2 cut(s)	421, 952
HindIII	AAGCTT	1 cut(s)	308
HinfI	GANTC	6 cut(s)	152, 251, 400, 457, 662, 1103
HpaII	CCGG	9 cut(s)	10, 52, 261, 363, 375, 470, 550, 693, 1111
HphI	GGTGA	5 cut(s)	419, 544, 749, 907, 1088
Hpy166II	GTNNAC	4 cut(s)	15, 276, 417, 547
Hpy188I	TCNGA	6 cut(s)	33, 159, 209, 282, 970, 1108
Hpy188III	TCNNGA	6 cut(s)	229, 286, 647, 693, 983, 1140
Hpy8I	GTNNAC	4 cut(s)	15, 276, 417, 547
Hpy99I	CGWCG	1 cut(s)	490
HpyAV	CCTTC	7 cut(s)	67, 173, 179, 241, 601, 886, 954
HpyCH4III	ACNGT	4 cut(s)	319, 595, 727, 761
HpyCH4IV	ACGT	1 cut(s)	413
HpyCH4V	TGCA	7 cut(s)	4, 429, 438, 587, 598, 686, 818
HpyF10VI	GCNNNNNNNGC	6 cut(s)	26, 143, 363, 375, 435, 958
HpyF3I	CTNAG	3 cut(s)	279, 296, 984
HpySE526I	ACGT	1 cut(s)	413
Hsp92II	CATG	7 cut(s)	628, 812, 940, 961, 1018, 1054, 1130
HspAI	GCGC	2 cut(s)	421, 952
Kpn2I	TCCGGA	1 cut(s)	692
Ksp22I	TGATCA	1 cut(s)	868
Kzo9I	GATC	6 cut(s)	33, 255, 282, 565, 754, 868
LguI	GCTCTTC	1 cut(s)	726
LmnI	GCTCC	2 cut(s)	573, 817
Lsp1109I	GCAGC	3 cut(s)	656, 839, 914
LweI	GCATC	1 cut(s)	1049
MaeI	CTAG	2 cut(s)	23, 540
MaeII	ACGT	1 cut(s)	413
MalI	GATC	6 cut(s)	35, 257, 284, 567, 756, 870
MbiI	CCGCTC	1 cut(s)	576
MboI	GATC	6 cut(s)	33, 255, 282, 565, 754, 868
MboII	GAAGA	4 cut(s)	229, 457, 743, 1113
MhlI	GDGCHC	3 cut(s)	139, 341, 1009
MlsI	TGGCCA	1 cut(s)	800
MluCI	AATT	3 cut(s)	107, 302, 766
MluNI	TGGCCA	1 cut(s)	800
MlyI	GAGTC	4 cut(s)	161, 260, 451, 656
MmeI	TCCRAC	1 cut(s)	187
Mox20I	TGGCCA	1 cut(s)	800
MroI	TCCGGA	1 cut(s)	692
MroXI	GAANNNNTTC	1 cut(s)	735
MscI	TGGCCA	1 cut(s)	800
MseI	TTAA	2 cut(s)	615, 791
MslI	CAYNNNNRTG	3 cut(s)	395, 962, 1019
Msp20I	TGGCCA	1 cut(s)	800
MspI	CCGG	9 cut(s)	10, 52, 261, 363, 375, 470, 550, 693, 1111
MspR9I	CCNGG	6 cut(s)	10, 52, 261, 454, 551, 1112
MvaI	CCWGG	1 cut(s)	454
MvnI	CGCG	2 cut(s)	421, 423
MwoI	GCNNNNNNNGC	6 cut(s)	26, 143, 363, 375, 435, 958
NciI	CCSGG	5 cut(s)	10, 52, 261, 551, 1112
NcoI	CCATGG	1 cut(s)	1050
NdeII	GATC	6 cut(s)	33, 255, 282, 565, 754, 868
NlaIII	CATG	7 cut(s)	628, 812, 940, 961, 1018, 1054, 1130
NlaIV	GGNNCC	6 cut(s)	137, 211, 379, 474, 645, 1048
NmeAIII	GCCGAG	2 cut(s)	175, 327
NspI	RCATGY	4 cut(s)	628, 940, 961, 1130
NspV	TTCGAA	1 cut(s)	947
PaeI	GCATGC	1 cut(s)	628
PaeR7I	CTCGAG	1 cut(s)	664
PaqCI	CACCTGC	1 cut(s)	896
PceI	AGGCCT	2 cut(s)	129, 989
PciSI	GCTCTTC	1 cut(s)	726
PcsI	WCGNNNNNNNCGW	2 cut(s)	180, 561
PdmI	GAANNNNTTC	1 cut(s)	735
PfeI	GAWTC	2 cut(s)	400, 1103
PflMI	CCANNNNNTGG	1 cut(s)	121
PfoI	TCCNGGA	1 cut(s)	452
PkrI	GCNGC	7 cut(s)	28, 148, 266, 368, 671, 854, 904
PleI	GAGTC	4 cut(s)	160, 259, 451, 656
PpsI	GAGTC	4 cut(s)	160, 259, 451, 656
Ppu21I	YACGTR	1 cut(s)	414
Psp124BI	GAGCTC	1 cut(s)	1009
Psp6I	CCWGG	1 cut(s)	452
PspFI	CCCAGC	1 cut(s)	620
PspGI	CCWGG	1 cut(s)	452
PspN4I	GGNNCC	6 cut(s)	137, 211, 379, 474, 645, 1048
PspOMI	GGGCCC	1 cut(s)	135
PspPI	GGNCC	8 cut(s)	54, 135, 136, 188, 360, 472, 547, 1046
PspXI	VCTCGAGB	1 cut(s)	664
PstI	CTGCAG	1 cut(s)	820
RsaI	GTAC	2 cut(s)	275, 418
RsaNI	GTAC	2 cut(s)	274, 417
RseI	CAYNNNNRTG	3 cut(s)	395, 962, 1019
SacI	GAGCTC	1 cut(s)	1009
SapI	GCTCTTC	1 cut(s)	726
SaqAI	TTAA	2 cut(s)	615, 791
SatI	GCNGC	7 cut(s)	27, 147, 265, 367, 670, 853, 903
Sau3AI	GATC	6 cut(s)	33, 255, 282, 565, 754, 868
Sau96I	GGNCC	8 cut(s)	54, 135, 136, 188, 360, 472, 547, 1046
SbfI	CCTGCAGG	1 cut(s)	820
SchI	GAGTC	4 cut(s)	161, 260, 451, 656
ScrFI	CCNGG	6 cut(s)	10, 52, 261, 454, 551, 1112
SdaI	CCTGCAGG	1 cut(s)	820
SduI	GDGCHC	3 cut(s)	139, 341, 1009
SfaNI	GCATC	1 cut(s)	1049
SfcI	CTRYAG	1 cut(s)	816
Sfr274I	CTCGAG	1 cut(s)	664
SfuI	TTCGAA	1 cut(s)	947
SinI	GGWCC	5 cut(s)	54, 360, 472, 547, 1046
SlaI	CTCGAG	1 cut(s)	664
SmiMI	CAYNNNNRTG	3 cut(s)	395, 962, 1019
SmlI	CTYRAG	1 cut(s)	664
SmoI	CTYRAG	1 cut(s)	664
SphI	GCATGC	1 cut(s)	628
Sse8387I	CCTGCAGG	1 cut(s)	820
Sse9I	AATT	3 cut(s)	107, 302, 766
SseBI	AGGCCT	2 cut(s)	129, 989
SspI	AATATT	1 cut(s)	718
SspMI	CTAG	2 cut(s)	23, 540
SstI	GAGCTC	1 cut(s)	1009
StuI	AGGCCT	2 cut(s)	129, 989
StyD4I	CCNGG	6 cut(s)	8, 50, 259, 452, 549, 1110
StyI	CCWWGG	1 cut(s)	1050
TaaI	ACNGT	4 cut(s)	319, 595, 727, 761
TaiI	ACGT	1 cut(s)	416
TaqI	TCGA	5 cut(s)	44, 254, 665, 947, 1030
TasI	AATT	3 cut(s)	107, 302, 766
TatI	WGTACW	1 cut(s)	273
TauI	GCSGC	4 cut(s)	29, 149, 267, 369
TfiI	GAWTC	2 cut(s)	400, 1103
Tru1I	TTAA	2 cut(s)	615, 791
Tru9I	TTAA	2 cut(s)	615, 791
TscAI	CASTG	2 cut(s)	391, 753
TseI	GCWGC	3 cut(s)	669, 852, 902
TspDTI	ATGAA	3 cut(s)	260, 495, 1107
TspGWI	ACGGA	1 cut(s)	182
TspRI	CASTG	2 cut(s)	391, 753
Van91I	CCANNNNNTGG	1 cut(s)	121
VpaK11BI	GGWCC	5 cut(s)	54, 360, 472, 547, 1046
XceI	RCATGY	4 cut(s)	628, 940, 961, 1130
XhoI	CTCGAG	1 cut(s)	664
XmnI	GAANNNNTTC	1 cut(s)	735
XspI	CTAG	2 cut(s)	23, 540

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.