MD09G1118500.v1.1

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0022352
Physical Location & Seq
Reverse (-)
9116148 .. 9116576
429 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1118500.v1.1.491

Sequence Viewer

Length: 429 bp
ATGAGTGTAGAAGTGTTGGACGGTGCCACCATTTTCAACTTTGTGGAAGATGAAGAAGCATTCAGCCTCTCAATTCGCGACCGTTTTGCTCATCTCGACACCAACCACGATGGTCTGCTTTCTTATGCAGAAATGCTGAAGGAGCTTCAGTCTCTGAGGGTCTTTGAGACCCACTATGGCATTGATGTGAAGCCAGACCCCGAAGAGATTGCTCATGTCTACGACTCGCTGTTCGTGCAGTTCGATCATGACTCGAACGGGGCGGTTGATTTGGAGGAGTTCAAGGCTGAAACCAAGCGGATGATGCTTGCTATGGCCAATGGGATGGGGTTCTTGCCAGTTCAAATGGTGTTGGAAGAAGATAGCTACTTGAAGAAAGCAGTGGAAAAAGAGTACTCCAGTACCAAAGTTAAGCAGCTGATTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

143

Amino Acids

16.24

Weight (kDa)

4.54

Isoelectric Point (pI)

38.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 25 - 96 3.6e-13 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022352)

Species Orthologous Gene IDs
malus_domestica MD09G1118000.v1.1 MD09G1118500.v1.1 MD17G1109300.v1.1
prunus_persica Prupe.3G205900_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 23
AccI GTMKAC 1 cut(s) 219
AccII CGCG 1 cut(s) 78
AciI CCGC 2 cut(s) 263, 298
AcoI YGGCCR 1 cut(s) 315
AcuI CTGAAG 2 cut(s) 131, 158
AfaI GTAC 2 cut(s) 395, 403
AgsI TTSAA 4 cut(s) 37, 283, 344, 373
AluBI AGCT 3 cut(s) 145, 366, 418
AluI AGCT 3 cut(s) 145, 366, 418
Alw26I GTCTC 2 cut(s) 156, 161
AlwNI CAGNNNCTG 1 cut(s) 154
AoxI GGCC 1 cut(s) 315
ApeKI GCWGC 1 cut(s) 415
ArsI GACNNNNNNTTYG 2 cut(s) 215, 247
AseI ATTAAT 1 cut(s) 423
BaeI ACNNNNGTAYC 2 cut(s) 385, 418
BalI TGGCCA 1 cut(s) 317
BanI GGYRCC 1 cut(s) 23
BccI CCATC 2 cut(s) 104, 319
BcgI CGANNNNNNTGC 4 cut(s) 68, 102, 191, 225
BcoDI GTCTC 2 cut(s) 156, 161
BisI GCNGC 1 cut(s) 416
BlsI GCNGC 1 cut(s) 417
BmcAI AGTACT 1 cut(s) 395
BmiI GGNNCC 1 cut(s) 25
BmsI GCATC 1 cut(s) 294
BpmI CTGGAG 1 cut(s) 382
BsaI GGTCTC 1 cut(s) 161
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bse1I ACTGG 2 cut(s) 338, 399
BseGI GGATG 2 cut(s) 306, 330
BseMII CTCAG 1 cut(s) 146
BseNI ACTGG 2 cut(s) 338, 399
BseRI GAGGAG 1 cut(s) 290
BsgI GTGCAG 1 cut(s) 257
Bsh1236I CGCG 1 cut(s) 78
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 1 cut(s) 317
BshNI GGYRCC 1 cut(s) 23
BsiEI CGRYCG 1 cut(s) 82
BsmAI GTCTC 2 cut(s) 156, 161
BsmI GAATGC 1 cut(s) 59
BsnI GGCC 1 cut(s) 317
Bso31I GGTCTC 1 cut(s) 161
Bsp143I GATC 1 cut(s) 244
Bsp68I TCGCGA 1 cut(s) 78
BspACI CCGC 2 cut(s) 263, 298
BspANI GGCC 1 cut(s) 317
BspCNI CTCAG 1 cut(s) 147
BspFNI CGCG 1 cut(s) 78
BspHI TCATGA 1 cut(s) 247
BspLI GGNNCC 1 cut(s) 25
BspT107I GGYRCC 1 cut(s) 23
BspTNI GGTCTC 1 cut(s) 161
BsrI ACTGG 2 cut(s) 338, 399
BssMI GATC 1 cut(s) 244
Bst4CI ACNGT 2 cut(s) 23, 83
Bst6I CTCTTC 1 cut(s) 198
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 1 cut(s) 155
BstF5I GGATG 2 cut(s) 306, 330
BstFNI CGCG 1 cut(s) 78
BstKTI GATC 1 cut(s) 247
BstMAI GTCTC 2 cut(s) 156, 161
BstMBI GATC 1 cut(s) 244
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 3 cut(s) 142, 235, 304
BstUI CGCG 1 cut(s) 78
BstXI CCANNNNNNTGG 1 cut(s) 325
BsuRI GGCC 1 cut(s) 317
BtsCI GGATG 2 cut(s) 306, 330
BtsI GCAGTG 1 cut(s) 387
BtsIMutI CAGTG 1 cut(s) 387
BtuMI TCGCGA 1 cut(s) 78
Cac8I GCNNGC 1 cut(s) 309
CaiI CAGNNNCTG 1 cut(s) 154
CciI TCATGA 1 cut(s) 247
Csp6I GTAC 2 cut(s) 394, 402
CviAII CATG 2 cut(s) 215, 248
CviJI RGCY 7 cut(s) 66, 145, 193, 287, 317, 366, 418
CviKI_1 RGCY 7 cut(s) 66, 145, 193, 287, 317, 366, 418
CviQI GTAC 2 cut(s) 394, 402
DdeI CTNAG 1 cut(s) 155
DpnI GATC 1 cut(s) 246
DpnII GATC 1 cut(s) 244
EaeI YGGCCR 1 cut(s) 315
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
Eco31I GGTCTC 1 cut(s) 161
Eco57I CTGAAG 2 cut(s) 131, 158
FaeI CATG 2 cut(s) 218, 251
FaiI YATR 5 cut(s) 126, 177, 216, 249, 314
FatI CATG 2 cut(s) 214, 247
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 1 cut(s) 416
FokI GGATG 2 cut(s) 313, 337
Fsp4HI GCNGC 1 cut(s) 416
GluI GCNGC 1 cut(s) 416
GsuI CTGGAG 1 cut(s) 382
HaeIII GGCC 1 cut(s) 317
Hin1II CATG 2 cut(s) 218, 251
HinfI GANTC 2 cut(s) 224, 251
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 1 cut(s) 156
Hpy188III TCNNGA 3 cut(s) 77, 95, 248
Hpy8I GTNNAC 1 cut(s) 220
HpyAV CCTTC 1 cut(s) 133
HpyCH4III ACNGT 2 cut(s) 23, 83
HpyCH4V TGCA 2 cut(s) 128, 238
HpyF10VI GCNNNNNNNGC 3 cut(s) 142, 235, 304
HpyF3I CTNAG 1 cut(s) 155
Hsp92II CATG 2 cut(s) 218, 251
Kzo9I GATC 1 cut(s) 244
LmnI GCTCC 1 cut(s) 142
LpnPI CCDG 3 cut(s) 207, 351, 412
LweI GCATC 1 cut(s) 294
MalI GATC 1 cut(s) 246
MboI GATC 1 cut(s) 244
MboII GAAGA 6 cut(s) 59, 65, 215, 368, 371, 385
MlsI TGGCCA 1 cut(s) 317
MluCI AATT 2 cut(s) 72, 424
MluNI TGGCCA 1 cut(s) 317
MlyI GAGTC 2 cut(s) 218, 245
MmeI TCCRAC 1 cut(s) 333
MnlI CCTC 3 cut(s) 77, 150, 268
Mox20I TGGCCA 1 cut(s) 317
MscI TGGCCA 1 cut(s) 317
MseI TTAA 3 cut(s) 411, 423, 427
MslI CAYNNNNRTG 1 cut(s) 185
Msp20I TGGCCA 1 cut(s) 317
MspA1I CMGCKG 1 cut(s) 418
Mva1269I GAATGC 1 cut(s) 59
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 3 cut(s) 142, 235, 304
NdeII GATC 1 cut(s) 244
NlaIII CATG 2 cut(s) 218, 251
NlaIV GGNNCC 1 cut(s) 25
NruI TCGCGA 1 cut(s) 78
PacI TTAATTAA 1 cut(s) 427
PagI TCATGA 1 cut(s) 247
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PctI GAATGC 1 cut(s) 59
PkrI GCNGC 1 cut(s) 417
PleI GAGTC 2 cut(s) 218, 245
PpsI GAGTC 2 cut(s) 218, 245
PshBI ATTAAT 1 cut(s) 423
PspN4I GGNNCC 1 cut(s) 25
PstNI CAGNNNCTG 1 cut(s) 154
PvuII CAGCTG 1 cut(s) 418
RruI TCGCGA 1 cut(s) 78
RsaI GTAC 2 cut(s) 395, 403
RsaNI GTAC 2 cut(s) 394, 402
RseI CAYNNNNRTG 1 cut(s) 185
SaqAI TTAA 3 cut(s) 411, 423, 427
SatI GCNGC 1 cut(s) 416
Sau3AI GATC 1 cut(s) 244
ScaI AGTACT 1 cut(s) 395
SchI GAGTC 2 cut(s) 218, 245
SetI ASST 3 cut(s) 147, 368, 420
SfaNI GCATC 1 cut(s) 294
SmiMI CAYNNNNRTG 1 cut(s) 185
Sse9I AATT 2 cut(s) 72, 424
SsiI CCGC 2 cut(s) 263, 298
TaaI ACNGT 2 cut(s) 23, 83
TaqI TCGA 3 cut(s) 96, 243, 254
TasI AATT 2 cut(s) 72, 424
TatI WGTACW 1 cut(s) 393
Tru1I TTAA 3 cut(s) 411, 423, 427
Tru9I TTAA 3 cut(s) 411, 423, 427
TscAI CASTG 1 cut(s) 387
TseI GCWGC 1 cut(s) 415
TspDTI ATGAA 1 cut(s) 66
TspRI CASTG 1 cut(s) 387
VspI ATTAAT 1 cut(s) 423
XmiI GTMKAC 1 cut(s) 219
ZrmI AGTACT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.