Prupe.3G205900_v2.0.a1

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0022352
Physical Location & Seq
Forward (+)
21330935 .. 21331484
550 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G205900.1

Sequence Viewer

Length: 423 bp
ATGAGCGTAGAAGTATTGGATGGTGCCACAATTGTCAACTTTGTGGAGGATGAAGAGGCATTCAACCTTGCGGTACATCATCGTTTTGGTAATCTTGACACTAACCATGATGGTCTACTTTGTTATGCAGAGATGTTGAAGGAGCTTCAGTCTCTGAGGGTCTTCGAGACACACTATGGCATTGATGTGAAGCCAGACCCAGAAGAGATTGCTCGTGTCTATGACTCTCTGTTCGTGCAGTTTGATCATGACTCAAATGGGGCCGTTGATTTGGAAGAGTTCAAGGCTGAAACCAAGCGTATGATGCTTGCTATGGCCAATGGGATGGGGTTCTTGCCAGTTCAAATGGTTTTGGAAGAAGATAGCTTCCTGAAGAAAGCTGTGGAAAGAGAGTCCACCCAAATCAAGCAGCTGCTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

141

Amino Acids

15.97

Weight (kDa)

4.52

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022352)

Species Orthologous Gene IDs
malus_domestica MD09G1118000.v1.1 MD09G1118500.v1.1 MD17G1109300.v1.1
prunus_persica Prupe.3G205900_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 23
AccI GTMKAC 1 cut(s) 115
AciI CCGC 1 cut(s) 71
AcoI YGGCCR 1 cut(s) 315
AcuI CTGAAG 2 cut(s) 131, 392
AfaI GTAC 1 cut(s) 75
AgsI TTSAA 4 cut(s) 64, 139, 283, 344
AluBI AGCT 4 cut(s) 145, 366, 380, 412
AluI AGCT 4 cut(s) 145, 366, 380, 412
Alw26I GTCTC 2 cut(s) 156, 161
AlwNI CAGNNNCTG 1 cut(s) 154
AoxI GGCC 2 cut(s) 261, 315
ApeKI GCWGC 2 cut(s) 409, 412
ArsI GACNNNNNNTTYG 2 cut(s) 215, 247
AspS9I GGNCC 1 cut(s) 261
BalI TGGCCA 1 cut(s) 317
BanI GGYRCC 1 cut(s) 23
BauI CACGAG 1 cut(s) 213
BbsI GAAGAC 1 cut(s) 154
BbvI GCAGC 1 cut(s) 399
BccI CCATC 3 cut(s) 14, 104, 319
BceAI ACGGC 1 cut(s) 248
BclI TGATCA 1 cut(s) 244
BcoDI GTCTC 2 cut(s) 156, 161
BisI GCNGC 2 cut(s) 410, 413
BlsI GCNGC 2 cut(s) 411, 414
BmgT120I GGNCC 1 cut(s) 261
BmiI GGNNCC 2 cut(s) 25, 262
BmsI GCATC 1 cut(s) 294
BpiI GAAGAC 1 cut(s) 154
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bse1I ACTGG 1 cut(s) 338
BseGI GGATG 3 cut(s) 25, 55, 330
BseMII CTCAG 1 cut(s) 146
BseNI ACTGG 1 cut(s) 338
BseXI GCAGC 1 cut(s) 399
BsgI GTGCAG 1 cut(s) 257
BshFI GGCC 2 cut(s) 263, 317
BshNI GGYRCC 1 cut(s) 23
BsmAI GTCTC 2 cut(s) 156, 161
BsmI GAATGC 1 cut(s) 59
BsnI GGCC 2 cut(s) 263, 317
Bsp143I GATC 1 cut(s) 244
BspACI CCGC 1 cut(s) 71
BspANI GGCC 2 cut(s) 263, 317
BspCNI CTCAG 1 cut(s) 147
BspHI TCATGA 1 cut(s) 247
BspLI GGNNCC 2 cut(s) 25, 262
BspT107I GGYRCC 1 cut(s) 23
BsrI ACTGG 1 cut(s) 338
BssMI GATC 1 cut(s) 244
BssSI CACGAG 1 cut(s) 213
Bst2BI CACGAG 1 cut(s) 213
Bst6I CTCTTC 3 cut(s) 48, 198, 270
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 1 cut(s) 155
BstF5I GGATG 3 cut(s) 25, 55, 330
BstKTI GATC 1 cut(s) 247
BstMAI GTCTC 2 cut(s) 156, 161
BstMBI GATC 1 cut(s) 244
BstMWI GCNNNNNNNGC 1 cut(s) 304
BstV1I GCAGC 1 cut(s) 399
BstV2I GAAGAC 1 cut(s) 154
BstXI CCANNNNNNTGG 1 cut(s) 325
BsuRI GGCC 2 cut(s) 263, 317
BtsCI GGATG 3 cut(s) 25, 55, 330
Cac8I GCNNGC 1 cut(s) 309
CaiI CAGNNNCTG 1 cut(s) 154
CciI TCATGA 1 cut(s) 247
Cfr13I GGNCC 1 cut(s) 261
Csp6I GTAC 1 cut(s) 74
CviAII CATG 2 cut(s) 107, 248
CviJI RGCY 8 cut(s) 145, 193, 263, 287, 317, 366, 380, 412
CviKI_1 RGCY 8 cut(s) 145, 193, 263, 287, 317, 366, 380, 412
CviQI GTAC 1 cut(s) 74
DdeI CTNAG 1 cut(s) 155
DpnI GATC 1 cut(s) 246
DpnII GATC 1 cut(s) 244
EaeI YGGCCR 1 cut(s) 315
Eam1104I CTCTTC 3 cut(s) 48, 198, 270
EarI CTCTTC 3 cut(s) 48, 198, 270
Eco57I CTGAAG 2 cut(s) 131, 392
FaeI CATG 2 cut(s) 110, 251
FaiI YATR 7 cut(s) 108, 126, 177, 222, 249, 302, 314
FatI CATG 2 cut(s) 106, 247
FbaI TGATCA 1 cut(s) 244
FblI GTMKAC 1 cut(s) 115
Fnu4HI GCNGC 2 cut(s) 410, 413
FokI GGATG 3 cut(s) 32, 62, 337
Fsp4HI GCNGC 2 cut(s) 410, 413
GluI GCNGC 2 cut(s) 410, 413
HaeIII GGCC 2 cut(s) 263, 317
Hin1II CATG 2 cut(s) 110, 251
HincII GTYRAC 1 cut(s) 37
HindII GTYRAC 1 cut(s) 37
HinfI GANTC 3 cut(s) 224, 251, 392
Hpy166II GTNNAC 3 cut(s) 37, 116, 396
Hpy188I TCNGA 1 cut(s) 156
Hpy188III TCNNGA 4 cut(s) 95, 166, 248, 370
Hpy8I GTNNAC 3 cut(s) 37, 116, 396
HpyAV CCTTC 1 cut(s) 133
HpyCH4V TGCA 2 cut(s) 128, 238
HpyF10VI GCNNNNNNNGC 1 cut(s) 304
HpyF3I CTNAG 1 cut(s) 155
Hsp92II CATG 2 cut(s) 110, 251
Ksp22I TGATCA 1 cut(s) 244
Kzo9I GATC 1 cut(s) 244
LmnI GCTCC 1 cut(s) 142
LpnPI CCDG 4 cut(s) 207, 213, 351, 383
Lsp1109I GCAGC 1 cut(s) 399
LweI GCATC 1 cut(s) 294
MalI GATC 1 cut(s) 246
MboI GATC 1 cut(s) 244
MboII GAAGA 7 cut(s) 65, 154, 215, 287, 368, 371, 385
MfeI CAATTG 1 cut(s) 30
MlsI TGGCCA 1 cut(s) 317
MluCI AATT 2 cut(s) 30, 418
MluNI TGGCCA 1 cut(s) 317
MlyI GAGTC 3 cut(s) 218, 245, 401
MnlI CCTC 3 cut(s) 40, 49, 150
Mox20I TGGCCA 1 cut(s) 317
MscI TGGCCA 1 cut(s) 317
MseI TTAA 2 cut(s) 417, 421
MslI CAYNNNNRTG 1 cut(s) 185
Msp20I TGGCCA 1 cut(s) 317
MspA1I CMGCKG 1 cut(s) 412
MunI CAATTG 1 cut(s) 30
Mva1269I GAATGC 1 cut(s) 59
MwoI GCNNNNNNNGC 1 cut(s) 304
NdeII GATC 1 cut(s) 244
NlaIII CATG 2 cut(s) 110, 251
NlaIV GGNNCC 2 cut(s) 25, 262
PacI TTAATTAA 1 cut(s) 421
PagI TCATGA 1 cut(s) 247
PctI GAATGC 1 cut(s) 59
PkrI GCNGC 2 cut(s) 411, 414
PleI GAGTC 3 cut(s) 218, 245, 400
PpsI GAGTC 3 cut(s) 218, 245, 400
PspN4I GGNNCC 2 cut(s) 25, 262
PspPI GGNCC 1 cut(s) 261
PstNI CAGNNNCTG 1 cut(s) 154
PvuII CAGCTG 1 cut(s) 412
RsaI GTAC 1 cut(s) 75
RsaNI GTAC 1 cut(s) 74
RseI CAYNNNNRTG 1 cut(s) 185
SaqAI TTAA 2 cut(s) 417, 421
SatI GCNGC 2 cut(s) 410, 413
Sau3AI GATC 1 cut(s) 244
Sau96I GGNCC 1 cut(s) 261
SchI GAGTC 3 cut(s) 218, 245, 401
SetI ASST 5 cut(s) 69, 147, 368, 382, 414
SfaNI GCATC 1 cut(s) 294
SmiMI CAYNNNNRTG 1 cut(s) 185
Sse9I AATT 2 cut(s) 30, 418
SsiI CCGC 1 cut(s) 71
TaqI TCGA 1 cut(s) 165
TasI AATT 2 cut(s) 30, 418
Tru1I TTAA 2 cut(s) 417, 421
Tru9I TTAA 2 cut(s) 417, 421
TseI GCWGC 2 cut(s) 409, 412
TspDTI ATGAA 1 cut(s) 66
XmiI GTMKAC 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.