MD17G1109300.v1.1

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0022352
Physical Location & Seq
Reverse (-)
9364649 .. 9365077
429 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1109300.v1.1.491

Sequence Viewer

Length: 429 bp
ATGAGTGTAGAAGTGCTGGATGGTGCCACCATTGTCAACTTTGTGGAAGATGAAGAAGCATTCACCCGCTCAGTACGGGACCGTTTTGCTTGTCTCGACACCAATCACGATGGTCTGCTTTCTTATACAGAGATGCTGAAGGAGCTTCAGTCTCTACGGGTCTTCGAGACCCACTATGGCATTGATGTGAAGCCAAACCCGGAAGAGATTGCTCATGTCTACGACTCGCTGTTCTTGCAGTTCGATCATGACTCCAACGGGACGGTTGATTTGGAGGAGTTCAAGGCTGAAACCAAGAGGATGATGCTGGCTATGGCCAATGGTATGGGGTTCTTGCCAGTTCAAATGGTGTTGGAAGAAGATAGCTTGCTGAGGAAAGCAGTGGAAAAGGAGCACTCCAGTGCCAAAACTAAGCAGCTGCTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

143

Amino Acids

16.2

Weight (kDa)

4.67

Isoelectric Point (pI)

49.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 25 - 96 4.2e-11 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022352)

Species Orthologous Gene IDs
malus_domestica MD09G1118000.v1.1 MD09G1118500.v1.1 MD17G1109300.v1.1
prunus_persica Prupe.3G205900_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 23
AccBSI CCGCTC 1 cut(s) 69
AccI GTMKAC 1 cut(s) 219
AciI CCGC 1 cut(s) 67
AcoI YGGCCR 1 cut(s) 315
AcuI CTGAAG 2 cut(s) 131, 158
AfaI GTAC 1 cut(s) 75
AgsI TTSAA 2 cut(s) 283, 344
AleI CACNNNNGTG 1 cut(s) 399
AluBI AGCT 3 cut(s) 145, 366, 418
AluI AGCT 3 cut(s) 145, 366, 418
Alw21I GWGCWC 1 cut(s) 396
Alw26I GTCTC 3 cut(s) 98, 156, 161
AoxI GGCC 1 cut(s) 315
ApeKI GCWGC 2 cut(s) 415, 418
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
AspS9I GGNCC 1 cut(s) 79
AsuC2I CCSGG 1 cut(s) 200
AsuHPI GGTGA 1 cut(s) 55
AvaII GGWCC 1 cut(s) 79
BalI TGGCCA 1 cut(s) 317
BanI GGYRCC 1 cut(s) 23
BbsI GAAGAC 1 cut(s) 154
Bbv12I GWGCWC 1 cut(s) 396
BbvCI CCTCAGC 1 cut(s) 371
BbvI GCAGC 1 cut(s) 405
BccI CCATC 2 cut(s) 14, 104
BcnI CCSGG 1 cut(s) 200
BcoDI GTCTC 3 cut(s) 98, 156, 161
BisI GCNGC 2 cut(s) 416, 419
BlsI GCNGC 2 cut(s) 417, 420
Bme1390I CCNGG 1 cut(s) 200
Bme18I GGWCC 1 cut(s) 79
BmgT120I GGNCC 1 cut(s) 79
BmiI GGNNCC 2 cut(s) 25, 80
BmrFI CCNGG 1 cut(s) 200
BmsI GCATC 2 cut(s) 123, 294
BpiI GAAGAC 1 cut(s) 154
BpmI CTGGAG 1 cut(s) 382
Bpu10I CCTNAGC 1 cut(s) 371
BpuMI CCSGG 1 cut(s) 200
BsaI GGTCTC 1 cut(s) 161
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bse1I ACTGG 2 cut(s) 338, 399
BseGI GGATG 2 cut(s) 25, 306
BseMII CTCAG 2 cut(s) 84, 362
BseNI ACTGG 2 cut(s) 338, 399
BseRI GAGGAG 1 cut(s) 290
BseXI GCAGC 1 cut(s) 405
BshFI GGCC 1 cut(s) 317
BshNI GGYRCC 1 cut(s) 23
BsiHKAI GWGCWC 1 cut(s) 396
BsiSI CCGG 1 cut(s) 200
BslFI GGGAC 2 cut(s) 92, 274
BsmAI GTCTC 3 cut(s) 98, 156, 161
BsmFI GGGAC 2 cut(s) 92, 274
BsmI GAATGC 1 cut(s) 59
BsnI GGCC 1 cut(s) 317
Bso31I GGTCTC 1 cut(s) 161
Bsp1286I GDGCHC 1 cut(s) 396
Bsp143I GATC 1 cut(s) 244
BspACI CCGC 1 cut(s) 67
BspANI GGCC 1 cut(s) 317
BspCNI CTCAG 2 cut(s) 83, 363
BspHI TCATGA 1 cut(s) 247
BspLI GGNNCC 2 cut(s) 25, 80
BspT107I GGYRCC 1 cut(s) 23
BspTNI GGTCTC 1 cut(s) 161
BsrBI CCGCTC 1 cut(s) 69
BsrI ACTGG 2 cut(s) 338, 399
BssMI GATC 1 cut(s) 244
Bst4CI ACNGT 2 cut(s) 83, 265
Bst6I CTCTTC 1 cut(s) 198
BstC8I GCNNGC 2 cut(s) 309, 368
BstDEI CTNAG 3 cut(s) 70, 371, 411
BstF5I GGATG 2 cut(s) 25, 306
BstKTI GATC 1 cut(s) 247
BstMAI GTCTC 3 cut(s) 98, 156, 161
BstMBI GATC 1 cut(s) 244
BstMWI GCNNNNNNNGC 2 cut(s) 142, 235
BstSCI CCNGG 1 cut(s) 198
BstV1I GCAGC 1 cut(s) 405
BstV2I GAAGAC 1 cut(s) 154
BstXI CCANNNNNNTGG 1 cut(s) 325
BsuRI GGCC 1 cut(s) 317
BtsCI GGATG 2 cut(s) 25, 306
BtsI GCAGTG 1 cut(s) 387
BtsIMutI CAGTG 2 cut(s) 387, 406
Cac8I GCNNGC 2 cut(s) 309, 368
CciI TCATGA 1 cut(s) 247
Cfr13I GGNCC 1 cut(s) 79
Csp6I GTAC 1 cut(s) 74
CviAII CATG 2 cut(s) 215, 248
CviJI RGCY 7 cut(s) 145, 193, 287, 311, 317, 366, 418
CviKI_1 RGCY 7 cut(s) 145, 193, 287, 311, 317, 366, 418
CviQI GTAC 1 cut(s) 74
DdeI CTNAG 3 cut(s) 70, 371, 411
DpnI GATC 1 cut(s) 246
DpnII GATC 1 cut(s) 244
EaeI YGGCCR 1 cut(s) 315
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
Eco31I GGTCTC 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 79
Eco57I CTGAAG 2 cut(s) 131, 158
FaeI CATG 2 cut(s) 218, 251
FaiI YATR 6 cut(s) 126, 177, 216, 249, 314, 326
FaqI GGGAC 2 cut(s) 92, 274
FatI CATG 2 cut(s) 214, 247
FauI CCCGC 1 cut(s) 74
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 2 cut(s) 416, 419
FokI GGATG 2 cut(s) 32, 313
Fsp4HI GCNGC 2 cut(s) 416, 419
GluI GCNGC 2 cut(s) 416, 419
GsuI CTGGAG 1 cut(s) 382
HaeIII GGCC 1 cut(s) 317
HapII CCGG 1 cut(s) 200
Hin1II CATG 2 cut(s) 218, 251
HincII GTYRAC 1 cut(s) 37
HindII GTYRAC 1 cut(s) 37
HinfI GANTC 2 cut(s) 224, 251
HpaII CCGG 1 cut(s) 200
HphI GGTGA 1 cut(s) 55
Hpy166II GTNNAC 2 cut(s) 37, 220
Hpy188III TCNNGA 4 cut(s) 95, 107, 166, 248
Hpy8I GTNNAC 2 cut(s) 37, 220
HpyAV CCTTC 1 cut(s) 133
HpyCH4III ACNGT 2 cut(s) 83, 265
HpyCH4V TGCA 1 cut(s) 238
HpyF10VI GCNNNNNNNGC 2 cut(s) 142, 235
HpyF3I CTNAG 3 cut(s) 70, 371, 411
Hsp92II CATG 2 cut(s) 218, 251
Kzo9I GATC 1 cut(s) 244
LmnI GCTCC 2 cut(s) 142, 391
LpnPI CCDG 5 cut(s) 2, 213, 293, 351, 412
Lsp1109I GCAGC 1 cut(s) 405
LweI GCATC 2 cut(s) 123, 294
MalI GATC 1 cut(s) 246
MbiI CCGCTC 1 cut(s) 69
MboI GATC 1 cut(s) 244
MboII GAAGA 6 cut(s) 59, 65, 154, 215, 368, 371
MhlI GDGCHC 1 cut(s) 396
MlsI TGGCCA 1 cut(s) 317
MluCI AATT 1 cut(s) 424
MluNI TGGCCA 1 cut(s) 317
MlyI GAGTC 2 cut(s) 218, 245
MmeI TCCRAC 2 cut(s) 279, 333
MnlI CCTC 3 cut(s) 268, 291, 366
Mox20I TGGCCA 1 cut(s) 317
MscI TGGCCA 1 cut(s) 317
MseI TTAA 2 cut(s) 423, 427
MslI CAYNNNNRTG 2 cut(s) 185, 399
Msp20I TGGCCA 1 cut(s) 317
MspA1I CMGCKG 1 cut(s) 418
MspI CCGG 1 cut(s) 200
MspR9I CCNGG 1 cut(s) 200
Mva1269I GAATGC 1 cut(s) 59
MwoI GCNNNNNNNGC 2 cut(s) 142, 235
NciI CCSGG 1 cut(s) 200
NdeII GATC 1 cut(s) 244
NlaIII CATG 2 cut(s) 218, 251
NlaIV GGNNCC 2 cut(s) 25, 80
OliI CACNNNNGTG 1 cut(s) 399
PacI TTAATTAA 1 cut(s) 427
PagI TCATGA 1 cut(s) 247
PctI GAATGC 1 cut(s) 59
PkrI GCNGC 2 cut(s) 417, 420
PleI GAGTC 2 cut(s) 218, 245
PpsI GAGTC 2 cut(s) 218, 245
PspN4I GGNNCC 2 cut(s) 25, 80
PspPI GGNCC 1 cut(s) 79
PvuII CAGCTG 1 cut(s) 418
RsaI GTAC 1 cut(s) 75
RsaNI GTAC 1 cut(s) 74
RseI CAYNNNNRTG 2 cut(s) 185, 399
SaqAI TTAA 2 cut(s) 423, 427
SatI GCNGC 2 cut(s) 416, 419
Sau3AI GATC 1 cut(s) 244
Sau96I GGNCC 1 cut(s) 79
SchI GAGTC 2 cut(s) 218, 245
ScrFI CCNGG 1 cut(s) 200
SduI GDGCHC 1 cut(s) 396
SetI ASST 3 cut(s) 147, 368, 420
SfaNI GCATC 2 cut(s) 123, 294
SinI GGWCC 1 cut(s) 79
SmiMI CAYNNNNRTG 2 cut(s) 185, 399
Sse9I AATT 1 cut(s) 424
SsiI CCGC 1 cut(s) 67
StyD4I CCNGG 1 cut(s) 198
TaaI ACNGT 2 cut(s) 83, 265
TaqI TCGA 3 cut(s) 96, 165, 243
TasI AATT 1 cut(s) 424
Tru1I TTAA 2 cut(s) 423, 427
Tru9I TTAA 2 cut(s) 423, 427
TscAI CASTG 2 cut(s) 387, 406
TseI GCWGC 2 cut(s) 415, 418
TspDTI ATGAA 1 cut(s) 66
TspRI CASTG 2 cut(s) 387, 406
VpaK11BI GGWCC 1 cut(s) 79
XmiI GTMKAC 1 cut(s) 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.