MD09G1129800.v1.1

Protein of unknown function (DUF1296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0020160
Physical Location & Seq
Forward (+)
10012448 .. 10021726
9279 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1129800.v1.1.491

Sequence Viewer

Length: 2613 bp
ATGAGTGGCGGTGGGCTTAGGGTTTCAATCCCCAACAGTGTGAGGAAGACGATCCAGGACATCAAGGAGATCACCGGCAATCACAGTGAGGAGGAAATCCACGCAATGCTCAAAGAATGCAACATGGATCCTGACGAGACCGCCCAGAAGCTTCTCTTTCAGGTGGGAAATCAGTTGGATCCATTTCATGAGGTCAAAAGGAAACGTGACAAAAGAAAAGAGAATCTGAACAACAAAGAGTCTGCAGAGTCCCGGTGGAGACCTGGAGGGCAGGGACGGGGGAGTAGGGGTGGTCGGGCGAATTTTACACCTCGTCATATACCACATGATGCTGGTGGTGGCAGAAATTCTGGTCCTGCAACAGAGAATGGACCCAGTCAGGGTGCCGAGAAAGGTGTTTCTCCCTCTTTCCCACCCTCTCGTGAGACAAAAAATAAAGAGAGAAGTGTCGATGGGCCTACCAATGTGGCTTTTGGAAGTACTAGTGTTGTGCATCCCTCCCATTCATCTGCTGGAAGTGGTCCTGAAATAAGTTTGTCACTGGTTGGGGATAATTTGGGAAGTTCGGTCCCCCCAGTGGATGCAAAAAAAACCCCCAACATTAAATACGGCAATGAGGACTTGCATGAGCAGCCTGCCCTAAGCTCCAGTAGCAGCTCAGTGTTGCCAACCCCAGCATCTACATTGGCAGCTTGTTTCTCCTCTTCAGATCCAGTACCCGTGCCATCTAATGATTCACGGCTCCCAGGTTCTGTGGGTACAATTAAATGTGAAATAGGGAGCCATCATCCTGCAGCTTCAGAGGTTGGTATCTCTCCGGCACAAGGTAAAGTGGCAAGCAAGACACAGGGAGTTGGGAAAAGTCAGCATTCTGAACTTTCACACTCTTCATCTGCATCAACTCATGGTGGTTCAGGGAGTCGGCCCCCATCTAATTACAGTAGCTGGTCACAACAATTGATTGGCACTCAAAAAGTTGGTACCAATAAAGAGTGGAAACCGAAACCAATCAATTCTGCTGTTGTTCAAGGTCTGGGAACGGCTGGTGCTGCTCCTGCATCTGAGGTTTCTGCCGATTCTGTAGAGGCCCCTTCTCAGTCACAGCTGGTTTCAAGAGTTCTTGATTCGGAAGAAGCAACTTCAAAACTGCAGAGAAAGCTAGAGGAATTCCACCTTCCTCAGCGCAAAGTAGTTATTCTTCCAAATCACATACATGTTCCAGAATCTGAAAGAACCAAGCTGAGTTTTGGAAGTTTTGGGGCCATGTTTGGTGTAAGTACTGGCTATGTGGGTTCCCCTGAGAGTGATAAGAACTCAACACCTTGCTCTGAGACTTCTCAGGTTGTTGAAGAATCTGTGGAGGAACAATCCTCAAGCAATCAAAATGCATTGGCAACTGCTGATGAAGGTGATTATCCTGATCATCCGCAATCACCTCCTCAAGTATCTGAAAATATATCAACTGGTGAGGGGGAAATATCATCCAGTGCGGCCCCAGGACACAATGAATCCAAGCATGACACTGCGTTGCCATCTGGAGGCCATCAGTTCTCGGTGGCTCATACTTCTCCGAACTACAGTTTTGGATTTGTGCCCCCAATTATAGGGAGTCAGCTTGCACCATTTGAAAACTCTGAGTCTCAATCACGTGATGTTTCCCGCCTTCCAGGCTTTGTTGTACAACCACCCTTTGACCCAGCAAGTTATTATGCCCAATATTACCGCTCAGGTGCTGATAGTGATGGCCGCCTTTCTCCATTTCCTTCTCCTGGGGTTTCCACCAAGTACAATGGGAATGTTGCAGTATTGCCTCAAAGTTCTCAGTCTCCCCAAGAGGGTGGGGTTCTATCTGCAACCGGCCCAACACCATTGGTGACACAGGCTCCTGGGCTTGTACAAAGCACTCAGCAGCCACTTCCTGTCTTTCGACCACCAGCTGGGATGCACATATCTCATTATCCTCCTAACTATATTCCCTACAGTCACTACTTTTCCCCATTCTATGTTCCACCTCCAGCCATCCACCAATATTTGGGCAATGGTGCATTTCCTCAGCAACCTCAAGCCGGTGGTGTGTATCCAGCTTCTCCAGCAGCAGCAGCAGCCGCAGGAGTTAAATACTCACTGCCACAATACAAAACAGGGACTAATTCAGGGAACTCAGCTCATATGGGAATGGCAAGTGGTTATGGGCCATCTGGTTCTTCCCTGGCTGGTTATAATCAGAGTGCTCCCACAACAGCAGGAAACTCTACTTCTAATGAGGATCTTGCCTCATCTCAGTTCAAGGAAAATAATGTATACATCACGGGACAGCAGAGTGAAGGATCTTCTGTGTGGGTTGCAGCACCTGGCCGAGACATATCCAGCTTAACAAGTTCGTTTTACAACCTTCCTCCTCAGGGTCAGCACATGACTTTCACCCCAACGCAGGCTGGCCATGGTACCTTTGCTGGTCTCTACCACCCTGCGCAAGCAGTAACAGCAGCAGCTGTTCATCCTCTTCTCCAGCAGTCCCAGACCATGGCCGGGGCTGTTGACATGGTGGGACCTGGGGGCAATGTTTATCAACAGCCTCAACATGCACAAATGAACTGGCCGAGTAACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1296

Protein Analysis

871

Amino Acids

91.24

Weight (kDa)

6.17

Isoelectric Point (pI)

63.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIP1_N PF06972 10 - 74 3.5e-30 GBF-interacting protein 1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020160)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07660
malus_domestica MD09G1129800.v1.1 MD17G1118800.v1.1
prunus_persica Prupe.3G195400_v2.0.a1
pyrus_communis pycom09g05290 pycom17g10910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2220
Acc16I TGCGCA 1 cut(s) 2472
Acc65I GGTACC 2 cut(s) 980, 2444
AccB1I GGYRCC 3 cut(s) 383, 980, 2444
AccB7I CCANNNNNTGG 3 cut(s) 1937, 2032, 2524
AccBSI CCGCTC 1 cut(s) 1725
AccI GTMKAC 1 cut(s) 2301
AciI CCGC 8 cut(s) 9, 141, 1427, 1490, 1660, 1723, 1747, 2106
AclWI GGATC 8 cut(s) 46, 122, 135, 173, 186, 704, 2274, 2335
AcoI YGGCCR 5 cut(s) 1744, 2353, 2437, 2526, 2597
AcsI RAATTY 3 cut(s) 301, 346, 1166
AcuI CTGAAG 2 cut(s) 690, 783
AcvI CACGTG 1 cut(s) 1649
AfaI GTAC 9 cut(s) 481, 717, 760, 982, 1279, 1680, 1787, 1896, 2446
AflIII ACRYGT 1 cut(s) 1213
AgsI TTSAA 7 cut(s) 27, 1028, 1113, 1143, 1349, 1628, 2287
AhlI ACTAGT 1 cut(s) 482
Alw21I GWGCWC 1 cut(s) 2233
Alw26I GTCTC 8 cut(s) 131, 253, 419, 1325, 1644, 1830, 2352, 2462
AlwI GGATC 8 cut(s) 46, 122, 135, 173, 186, 704, 2274, 2335
AlwNI CAGNNNCTG 6 cut(s) 752, 945, 1226, 1733, 2351, 2492
ApoI RAATTY 3 cut(s) 301, 346, 1166
Asp718I GGTACC 2 cut(s) 980, 2444
AspLEI GCGC 2 cut(s) 1185, 2473
AsuC2I CCSGG 2 cut(s) 253, 2530
AsuHPI GGTGA 6 cut(s) 64, 1421, 1425, 1478, 1885, 2413
AvaII GGWCC 5 cut(s) 353, 371, 521, 568, 2549
AxyI CCTNAGG 1 cut(s) 2400
BaeGI GKGCMC 1 cut(s) 1596
BalI TGGCCA 1 cut(s) 2439
BamHI GGATCC 2 cut(s) 127, 178
BanI GGYRCC 3 cut(s) 383, 980, 2444
BarI GAAGNNNNNNTAC 2 cut(s) 1182, 1214
BauI CACGAG 1 cut(s) 420
BbrPI CACGTG 1 cut(s) 1649
BbsI GAAGAC 1 cut(s) 53
Bbv12I GWGCWC 1 cut(s) 2233
BbvCI CCTCAGC 2 cut(s) 1179, 2052
BccI CCATC 9 cut(s) 446, 733, 792, 937, 1540, 1551, 1736, 2027, 2203
BceAI ACGGC 3 cut(s) 625, 755, 1056
BciVI GTATCC 1 cut(s) 2088
BclI TGATCA 1 cut(s) 1420
BcnI CCSGG 2 cut(s) 253, 2530
BcoDI GTCTC 8 cut(s) 131, 253, 419, 1325, 1644, 1830, 2352, 2462
BcuI ACTAGT 1 cut(s) 482
BfaI CTAG 2 cut(s) 483, 1160
BfmI CTRYAG 6 cut(s) 243, 792, 1080, 1148, 1576, 1978
BfuI GTATCC 1 cut(s) 2088
BglI GCCNNNNNGGC 1 cut(s) 1668
BmcAI AGTACT 2 cut(s) 481, 1279
Bme18I GGWCC 5 cut(s) 353, 371, 521, 568, 2549
BmrI ACTGGG 2 cut(s) 369, 569
BmsI GCATC 7 cut(s) 319, 502, 571, 686, 905, 1067, 1932
BmuI ACTGGG 2 cut(s) 369, 569
BpiI GAAGAC 1 cut(s) 53
BpmI CTGGAG 6 cut(s) 285, 631, 1557, 1998, 2073, 2492
Bpu10I CCTNAGC 5 cut(s) 17, 641, 1179, 1726, 2052
BpuEI CTTGAG 3 cut(s) 1357, 1425, 2046
BpuMI CCSGG 2 cut(s) 253, 2530
BsaAI YACGTR 1 cut(s) 1649
BsaI GGTCTC 3 cut(s) 131, 253, 2462
BsaJI CCNNGG 9 cut(s) 745, 1495, 1769, 1886, 2208, 2440, 2523, 2529, 2552
BsaXI ACNNNNNCTCC 4 cut(s) 274, 304, 1867, 1897
Bse118I RCCGGY 3 cut(s) 74, 1856, 2066
Bse1I ACTGG 9 cut(s) 375, 546, 575, 648, 713, 1285, 1468, 1485, 2600
Bse21I CCTNAGG 1 cut(s) 2400
Bse3DI GCAATG 4 cut(s) 111, 619, 2044, 2566
BseDI CCNNGG 9 cut(s) 745, 1495, 1769, 1886, 2208, 2440, 2523, 2529, 2552
BseGI GGATG 8 cut(s) 493, 586, 787, 1423, 1481, 1947, 2019, 2497
BseMI GCAATG 4 cut(s) 111, 619, 2044, 2566
BseNI ACTGG 9 cut(s) 375, 546, 575, 648, 713, 1285, 1468, 1485, 2600
BseRI GAGGAG 4 cut(s) 104, 691, 1428, 2388
BseSI GKGCMC 1 cut(s) 1596
BseYI CCCAGC 3 cut(s) 673, 1696, 1937
BshNI GGYRCC 3 cut(s) 383, 980, 2444
BsiHKAI GWGCWC 1 cut(s) 2233
BsiSI CCGG 6 cut(s) 75, 253, 818, 1857, 2067, 2529
BslFI GGGAC 7 cut(s) 235, 288, 554, 2158, 2325, 2500, 2562
BsmAI GTCTC 8 cut(s) 131, 253, 419, 1325, 1644, 1830, 2352, 2462
BsmFI GGGAC 7 cut(s) 235, 288, 554, 2158, 2325, 2500, 2562
BsmI GAATGC 2 cut(s) 122, 868
Bso31I GGTCTC 3 cut(s) 131, 253, 2462
Bsp1286I GDGCHC 2 cut(s) 1596, 2233
Bsp1407I TGTACA 2 cut(s) 1678, 1894
Bsp143I GATC 8 cut(s) 51, 69, 127, 178, 709, 1420, 2266, 2327
Bsp19I CCATGG 2 cut(s) 2440, 2523
BspACI CCGC 8 cut(s) 9, 141, 1427, 1490, 1660, 1723, 1747, 2106
BspHI TCATGA 1 cut(s) 187
BspMAI CTGCAG 3 cut(s) 247, 796, 1152
BspPI GGATC 8 cut(s) 46, 122, 135, 173, 186, 704, 2274, 2335
BspT107I GGYRCC 3 cut(s) 383, 980, 2444
BspTNI GGTCTC 3 cut(s) 131, 253, 2462
BsrBI CCGCTC 1 cut(s) 1725
BsrDI GCAATG 4 cut(s) 111, 619, 2044, 2566
BsrFI RCCGGY 3 cut(s) 74, 1856, 2066
BsrGI TGTACA 2 cut(s) 1678, 1894
BsrI ACTGG 9 cut(s) 375, 546, 575, 648, 713, 1285, 1468, 1485, 2600
BssAI RCCGGY 3 cut(s) 74, 1856, 2066
BssECI CCNNGG 9 cut(s) 745, 1495, 1769, 1886, 2208, 2440, 2523, 2529, 2552
BssMI GATC 8 cut(s) 51, 69, 127, 178, 709, 1420, 2266, 2327
BssNAI GTATAC 1 cut(s) 2302
BssSI CACGAG 1 cut(s) 420
BssT1I CCWWGG 2 cut(s) 2440, 2523
Bst1107I GTATAC 1 cut(s) 2302
Bst2BI CACGAG 1 cut(s) 420
Bst4CI ACNGT 5 cut(s) 38, 86, 941, 1580, 1982
Bst6I CTCTTC 3 cut(s) 709, 892, 2508
BstAUI TGTACA 2 cut(s) 1678, 1894
BstBAI YACGTR 1 cut(s) 1649
BstC8I GCNNGC 6 cut(s) 636, 838, 1617, 2433, 2437, 2475
BstDSI CCRYGG 2 cut(s) 2440, 2523
BstF5I GGATG 8 cut(s) 493, 586, 787, 1423, 1481, 1947, 2019, 2497
BstHHI GCGC 2 cut(s) 1185, 2473
BstKTI GATC 8 cut(s) 54, 72, 130, 181, 712, 1423, 2269, 2330
BstMAI GTCTC 8 cut(s) 131, 253, 419, 1325, 1644, 1830, 2352, 2462
BstMBI GATC 8 cut(s) 51, 69, 127, 178, 709, 1420, 2266, 2327
BstNSI RCATGY 2 cut(s) 1217, 2585
BstSFI CTRYAG 6 cut(s) 243, 792, 1080, 1148, 1576, 1978
BstSLI GKGCMC 1 cut(s) 1596
BstV2I GAAGAC 1 cut(s) 53
BstX2I RGATCY 5 cut(s) 127, 178, 709, 2266, 2327
BstXI CCANNNNNNTGG 1 cut(s) 1838
BstYI RGATCY 5 cut(s) 127, 178, 709, 2266, 2327
BstZ17I GTATAC 1 cut(s) 2302
Bsu36I CCTNAGG 1 cut(s) 2400
BsuI GTATCC 1 cut(s) 2088
BtgI CCRYGG 2 cut(s) 2440, 2523
BtsCI GGATG 8 cut(s) 493, 586, 787, 1423, 1481, 1947, 2019, 2497
BtsI GCAGTG 2 cut(s) 1521, 2123
BtsIMutI CAGTG 8 cut(s) 43, 91, 539, 582, 666, 1492, 1521, 2123
Cac8I GCNNGC 6 cut(s) 636, 838, 1617, 2433, 2437, 2475
CaiI CAGNNNCTG 6 cut(s) 752, 945, 1226, 1733, 2351, 2492
CciI TCATGA 1 cut(s) 187
CfoI GCGC 2 cut(s) 1185, 2473
Cfr10I RCCGGY 3 cut(s) 74, 1856, 2066
Csp6I GTAC 9 cut(s) 480, 716, 759, 981, 1278, 1679, 1786, 1895, 2445
CviQI GTAC 9 cut(s) 480, 716, 759, 981, 1278, 1679, 1786, 1895, 2445
DpnI GATC 8 cut(s) 53, 71, 129, 180, 711, 1422, 2268, 2329
DpnII GATC 8 cut(s) 51, 69, 127, 178, 709, 1420, 2266, 2327
EaeI YGGCCR 5 cut(s) 1744, 2353, 2437, 2526, 2597
Eam1104I CTCTTC 3 cut(s) 709, 892, 2508
EarI CTCTTC 3 cut(s) 709, 892, 2508
Eco130I CCWWGG 2 cut(s) 2440, 2523
Eco31I GGTCTC 3 cut(s) 131, 253, 2462
Eco47I GGWCC 5 cut(s) 353, 371, 521, 568, 2549
Eco57I CTGAAG 2 cut(s) 690, 783
Eco72I CACGTG 1 cut(s) 1649
Eco81I CCTNAGG 1 cut(s) 2400
EcoO109I RGGNCCY 2 cut(s) 1087, 2549
EcoRI GAATTC 1 cut(s) 1166
EcoT14I CCWWGG 2 cut(s) 2440, 2523
EcoT22I ATGCAT 1 cut(s) 1390
ErhI CCWWGG 2 cut(s) 2440, 2523
FalI AAGNNNNNCTT 2 cut(s) 140, 172
FaqI GGGAC 7 cut(s) 235, 288, 554, 2158, 2325, 2500, 2562
FauI CCCGC 1 cut(s) 1667
FauNDI CATATG 1 cut(s) 2169
FbaI TGATCA 1 cut(s) 1420
FblI GTMKAC 1 cut(s) 2301
FokI GGATG 8 cut(s) 480, 593, 774, 1410, 1468, 1954, 2006, 2484
FspBI CTAG 2 cut(s) 483, 1160
FspI TGCGCA 1 cut(s) 2472
GlaI GCGC 2 cut(s) 1184, 2472
GsaI CCCAGC 3 cut(s) 677, 1700, 1941
GsuI CTGGAG 6 cut(s) 285, 631, 1557, 1998, 2073, 2492
HapII CCGG 6 cut(s) 75, 253, 818, 1857, 2067, 2529
HhaI GCGC 2 cut(s) 1185, 2473
Hin6I GCGC 2 cut(s) 1183, 2471
HinP1I GCGC 2 cut(s) 1183, 2471
HincII GTYRAC 1 cut(s) 2539
HindII GTYRAC 1 cut(s) 2539
HindIII AAGCTT 1 cut(s) 149
HpaII CCGG 6 cut(s) 75, 253, 818, 1857, 2067, 2529
HphI GGTGA 6 cut(s) 64, 1421, 1425, 1478, 1885, 2413
Hpy166II GTNNAC 2 cut(s) 2302, 2539
Hpy188III TCNNGA 9 cut(s) 131, 188, 422, 524, 1113, 1121, 1220, 1418, 1536
Hpy8I GTNNAC 2 cut(s) 2302, 2539
HpyAV CCTTC 7 cut(s) 1101, 1184, 1400, 1673, 1773, 2318, 2402
HpyCH4III ACNGT 5 cut(s) 38, 86, 941, 1580, 1982
HpyCH4IV ACGT 2 cut(s) 205, 1648
HpySE526I ACGT 2 cut(s) 205, 1648
HspAI GCGC 2 cut(s) 1183, 2471
KpnI GGTACC 2 cut(s) 984, 2448
Ksp22I TGATCA 1 cut(s) 1420
Kzo9I GATC 8 cut(s) 51, 69, 127, 178, 709, 1420, 2266, 2327
LmnI GCTCC 6 cut(s) 650, 747, 780, 1057, 1888, 2236
LweI GCATC 7 cut(s) 319, 502, 571, 686, 905, 1067, 1932
MaeI CTAG 2 cut(s) 483, 1160
MaeII ACGT 2 cut(s) 205, 1648
MaeIII GTNAC 8 cut(s) 206, 537, 948, 1098, 1873, 1982, 2479, 2603
MalI GATC 8 cut(s) 53, 71, 129, 180, 711, 1422, 2268, 2329
MbiI CCGCTC 1 cut(s) 1725
MboI GATC 8 cut(s) 51, 69, 127, 178, 709, 1420, 2266, 2327
MboII GAAGA 9 cut(s) 58, 696, 879, 1142, 1190, 1361, 2196, 2322, 2495
MfeI CAATTG 1 cut(s) 956
MflI RGATCY 5 cut(s) 127, 178, 709, 2266, 2327
MhlI GDGCHC 2 cut(s) 1596, 2233
MlsI TGGCCA 1 cut(s) 2439
MluNI TGGCCA 1 cut(s) 2439
MlyI GAGTC 5 cut(s) 248, 257, 928, 1618, 1646
MmeI TCCRAC 1 cut(s) 156
Mox20I TGGCCA 1 cut(s) 2439
Mph1103I ATGCAT 1 cut(s) 1390
MscI TGGCCA 1 cut(s) 2439
MseI TTAA 4 cut(s) 603, 765, 2115, 2372
MslI CAYNNNNRTG 1 cut(s) 1212
Msp20I TGGCCA 1 cut(s) 2439
MspA1I CMGCKG 3 cut(s) 1105, 1937, 2492
MspI CCGG 6 cut(s) 75, 253, 818, 1857, 2067, 2529
MunI CAATTG 1 cut(s) 956
Mva1269I GAATGC 2 cut(s) 122, 868
NciI CCSGG 2 cut(s) 253, 2530
NcoI CCATGG 2 cut(s) 2440, 2523
NdeI CATATG 1 cut(s) 2169
NdeII GATC 8 cut(s) 51, 69, 127, 178, 709, 1420, 2266, 2327
NmeAIII GCCGAG 2 cut(s) 412, 2381
NmuCI GTSAC 6 cut(s) 206, 537, 948, 1098, 1873, 1982
NsbI TGCGCA 1 cut(s) 2472
NsiI ATGCAT 1 cut(s) 1390
NspI RCATGY 2 cut(s) 1217, 2585
PagI TCATGA 1 cut(s) 187
PciI ACATGT 1 cut(s) 1213
PctI GAATGC 2 cut(s) 122, 868
PfeI GAWTC 7 cut(s) 223, 734, 1076, 1124, 1223, 1352, 1508
PflFI GACNNNGTC 1 cut(s) 375
PflMI CCANNNNNTGG 3 cut(s) 1937, 2032, 2524
PfoI TCCNGGA 1 cut(s) 54
PleI GAGTC 5 cut(s) 247, 256, 927, 1617, 1645
PmaCI CACGTG 1 cut(s) 1649
PmlI CACGTG 1 cut(s) 1649
PpsI GAGTC 5 cut(s) 247, 256, 927, 1617, 1645
Ppu21I YACGTR 1 cut(s) 1649
PpuMI RGGWCCY 1 cut(s) 2549
PscI ACATGT 1 cut(s) 1213
PsiI TTATAA 1 cut(s) 2220
Psp5II RGGWCCY 1 cut(s) 2549
PspCI CACGTG 1 cut(s) 1649
PspFI CCCAGC 3 cut(s) 673, 1696, 1937
PspPPI RGGWCCY 1 cut(s) 2549
PstI CTGCAG 3 cut(s) 247, 796, 1152
PstNI CAGNNNCTG 6 cut(s) 752, 945, 1226, 1733, 2351, 2492
PsuI RGATCY 5 cut(s) 127, 178, 709, 2266, 2327
PsyI GACNNNGTC 1 cut(s) 375
PvuII CAGCTG 3 cut(s) 1105, 1937, 2492
RsaI GTAC 9 cut(s) 481, 717, 760, 982, 1279, 1680, 1787, 1896, 2446
RsaNI GTAC 9 cut(s) 480, 716, 759, 981, 1278, 1679, 1786, 1895, 2445
RseI CAYNNNNRTG 1 cut(s) 1212
SaqAI TTAA 4 cut(s) 603, 765, 2115, 2372
Sau3AI GATC 8 cut(s) 51, 69, 127, 178, 709, 1420, 2266, 2327
ScaI AGTACT 2 cut(s) 481, 1279
SchI GAGTC 5 cut(s) 248, 257, 928, 1618, 1646
SduI GDGCHC 2 cut(s) 1596, 2233
SfaNI GCATC 7 cut(s) 319, 502, 571, 686, 905, 1067, 1932
SfcI CTRYAG 6 cut(s) 243, 792, 1080, 1148, 1576, 1978
SinI GGWCC 5 cut(s) 353, 371, 521, 568, 2549
SmiMI CAYNNNNRTG 1 cut(s) 1212
SmlI CTYRAG 3 cut(s) 1372, 1440, 2061
SmoI CTYRAG 3 cut(s) 1372, 1440, 2061
SpeI ACTAGT 1 cut(s) 482
SsiI CCGC 8 cut(s) 9, 141, 1427, 1490, 1660, 1723, 1747, 2106
SspI AATATT 2 cut(s) 1718, 2030
SspMI CTAG 2 cut(s) 483, 1160
StyI CCWWGG 2 cut(s) 2440, 2523
TaaI ACNGT 5 cut(s) 38, 86, 941, 1580, 1982
TaiI ACGT 2 cut(s) 208, 1651
TaqI TCGA 2 cut(s) 450, 1927
TaqII GACCGA 1 cut(s) 556
TatI WGTACW 5 cut(s) 479, 1277, 1678, 1785, 1894
TauI GCSGC 3 cut(s) 1493, 1749, 2108
TfiI GAWTC 7 cut(s) 223, 734, 1076, 1124, 1223, 1352, 1508
Tru1I TTAA 4 cut(s) 603, 765, 2115, 2372
Tru9I TTAA 4 cut(s) 603, 765, 2115, 2372
TscAI CASTG 8 cut(s) 43, 91, 546, 582, 666, 1492, 1528, 2130
TseFI GTSAC 6 cut(s) 206, 537, 948, 1098, 1873, 1982
Tsp45I GTSAC 6 cut(s) 206, 537, 948, 1098, 1873, 1982
TspDTI ATGAA 7 cut(s) 176, 495, 879, 1419, 1521, 2486, 2606
TspRI CASTG 8 cut(s) 43, 91, 546, 582, 666, 1492, 1528, 2130
Tth111I GACNNNGTC 1 cut(s) 375
Van91I CCANNNNNTGG 3 cut(s) 1937, 2032, 2524
VpaK11BI GGWCC 5 cut(s) 353, 371, 521, 568, 2549
XapI RAATTY 3 cut(s) 301, 346, 1166
XceI RCATGY 2 cut(s) 1217, 2585
XcmI CCANNNNNNNNNTGG 1 cut(s) 509
XmiI GTMKAC 1 cut(s) 2301
XspI CTAG 2 cut(s) 483, 1160
ZrmI AGTACT 2 cut(s) 481, 1279
Zsp2I ATGCAT 1 cut(s) 1390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.