pycom09g05290

Protein of unknown function (DUF1296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0020160
Physical Location & Seq
Forward (+)
3938453 .. 3946691
8239 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g05290.8

Sequence Viewer

Length: 2595 bp
ATGAGTGGCGCTGGGCTTAGGGTTTCAATCCCCAACAGTGTGAGGAAGACGATCGAGCACATCAAGGAGATCACCGGCAATCACAGTGAGGAGGAAATCCACGCAATGCTCAAAGAATGCAACATGGATCCTAACGAGACCGCCCAGCAGCTTCTCTTTCAGGATCCATTTCATGAGGTCAAAAGGAAACGTGACAAAAGAAAAGAGAATCTGAACAACAAAGAATCTGCAGAGTCCCGGTGGAGACCTGGAGGGCAGGGACGGGGGAGTAGGGGCGGTCGGGCAAATTTTGCACCTCGTCATATGCCACATGATGCTGGTGGTGGCAGAAATTCTGGTCCTGCAACAGAGAATGGACCCAGTCAGGGTGCCGAGAAAGGTGTTTCTCCCTCTTTCCCACCCTCTCGTGAGACAAAAAATAAAGAGAGAAGTGTCGATGGGCCTACCAATGTGGCTTTTGGAAGTACTAGTGCTGTGCATCCCTCCCATTTATCTGCTGGAAGTGGTCCTGAAATAAGTTTGTCACCGGTTGGGGATAATTTGGGTAGTTCGGTCCCCCCAGTGGATGCAAAAAAAACCCCCAACATTAAATACGGCAATGAGGACTTGCATGAGCAGCCTGGCCTAAGCTCCAGTAGCAGCTCAGTGTTGCCAACCCCAGCATCTACATTGGCAGCTTGTTTCTCCTCTTCAGATCCAGCACTCGTGCCATCTAATGATTCATGGGTCCCAGGTTCTGTGGGTACAATTAAATGTGAAGTAGGGAGCCATCATCCTGCAGCTTCAGAGGTTGGTAGTTCTCCTGCACAAGTAAAAGTGGCAAGCAAGACACAGGGAGTTGGGAAAAGTCAGCATTCTGAACTTTCACACTCTTCATCTGCATCAACTCATGGTGGTTCCGGGAGTCGGCCCCCATCTAATTACAGTAGCCGGTCCCAACAATTGATTGGCACTCAAAAAGTTGGTACCAATAAAGAGTGGAAACCGAAACCAATCAATTCTGCTGTTGTTCAAGGTCTGGGAACAGCTGGTGCTGCTCCTGCATCTGAGGTTTCTGCCGATTCTGTAGAGGCCCCTTCTCAGTCACAGCTGGTTTCAAGAGTCCTTGATTCGGAAGAAGCAACTTCAAAACTGCAGAGAAAGCTAGAGGAATTACACCTTCCTCAGCGCAAAGTAGTTATTCTTCCAAATCACATACATGTTCCAGAATCTGAAAGAACCAAGTTGAGTTTTGGAAGTTTTGGGGCTATGTTTGGTGTAAGTACTGGCTATGTGGGTTCTCCTGAGAGTGATAAGAACTCAACACCTCACTCTGAGACTTCTCAGGTTGTTGAAGAATCTGTGGAGGAACAATCCTCAAGCAATCAAAATGCATTGGCCACTGCTGATGAAGGTGATTATCCTGATCATCCGCAATCACCTCAAGTATCTGAAAATATATCATCTGGTGAGGGGGAAGTATCATCCAGTGCAGCCGCAGGACACAATGAATCCAAGCATGACACTGCATTGCCGTCTGGAGGCCATCAGTTCTCGGTGGCTCATACCTCTCCGAACTACAGTTTTGGAATTGTGCCCCCAATTATAGGGAGTCAGCTTGCACCATTTGAAAACTCTGAGTCTCAATCACGTGATGTTTCCCGCCTTCCAGGCTTTGTAGTGCAACCACCCTTTGACCCAGCAAGTTATTATGCCCAATATTACCGCTCAGGTGCTGATAGTGATGGCCGCCTTTCTCCATTTCTTTCTCCTGGGGTTTCCACCAAGTACAATGGGAATGTTGCAGTATTGCCTCAAAGTTCTCAGTCTCCCCAAGAGGGCGGGGTTCTATCTGCAACCGGCCCAACACCATTGGTGACACAGGCTCCTGGGCTTGTGCAAAGCACTCAGCAGCCACTTCCTGTCTTTCGATCACCAGCTGGGATGCACTTATCTCATTATCCTCCTAACTATATTCCCTACAGTCACTACTTTTCCCCATTCTATGTTCCACCTCCAGCCATCCACCAATATTTGGGCAATGGTGCATTTCCTCAGCAACCTCAAGCCGGTGGTGTGTATCCAGCTCCGTCAGCAGCAGCAGCAGCCGCAGGAGTTAAATACTCACTTCCACCATACAAAACAGGGACTAATACAGGGAACTCCGCTCATATGGGAATGGGAAGTGGTTATGGGCCATCTGGTTCTACCCCGGCTGGTTATAATCAGAGTGCTCCCACGACAGCAGGAAACTCTACTTCTAATGAGGATCTTGCCTCATCTCAGTTCAAGGAAAATAATGTATACATCACGGGACAGCAGAGTGAAGGATCGTCTGTGTGGGTTGCAGCACCTGGCCGAGACGTATCCAGCTTAACAAGTTCGTTTTACAACCTTCCTCCTCAGGGTCAGCATGTGACTTTCACCCCAACGCAGGCTGGCCATGGTACCTTTGCTGGTCTCTACCACCCTGCGCAAGCAGTAACAGCAGCAACTGTTCATCCACTTCTCCAACAGTCCCAGACCATGGCCGGGGCTGTTGACATGGTGGGACCTGGGGGCAATGTTTATCAACAGCCTCAACATGCACAAATGAACTGGCCGAGTAACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1296

Protein Analysis

865

Amino Acids

90.47

Weight (kDa)

6.21

Isoelectric Point (pI)

64.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020160)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07660
malus_domestica MD09G1129800.v1.1 MD17G1118800.v1.1
prunus_persica Prupe.3G195400_v2.0.a1
pyrus_communis pycom09g05290 pycom17g10910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2202
Acc16I TGCGCA 1 cut(s) 2454
Acc65I GGTACC 2 cut(s) 965, 2426
AccB1I GGYRCC 3 cut(s) 368, 965, 2426
AccB7I CCANNNNNTGG 2 cut(s) 2014, 2506
AccBSI CCGCTC 2 cut(s) 1707, 2147
AccI GTMKAC 1 cut(s) 2283
AclWI GGATC 7 cut(s) 122, 135, 158, 171, 689, 2256, 2317
AcoI YGGCCR 6 cut(s) 1377, 1726, 2335, 2419, 2508, 2579
AcsI RAATTY 2 cut(s) 286, 331
AcuI CTGAAG 2 cut(s) 675, 768
AcvI CACGTG 1 cut(s) 1631
AfaI GTAC 6 cut(s) 466, 745, 967, 1264, 1769, 2428
AflIII ACRYGT 1 cut(s) 1198
AgeI ACCGGT 1 cut(s) 526
AgsI TTSAA 7 cut(s) 27, 1013, 1098, 1128, 1334, 1610, 2269
AhlI ACTAGT 1 cut(s) 467
AjnI CCWGG 8 cut(s) 247, 619, 730, 1648, 1750, 1867, 2332, 2533
Alw21I GWGCWC 2 cut(s) 60, 2215
Alw26I GTCTC 8 cut(s) 131, 238, 404, 1310, 1626, 1812, 2334, 2444
AlwI GGATC 7 cut(s) 122, 135, 158, 171, 689, 2256, 2317
AlwNI CAGNNNCTG 5 cut(s) 737, 1211, 1715, 2333, 2474
ApoI RAATTY 2 cut(s) 286, 331
AsiGI ACCGGT 1 cut(s) 526
Asp718I GGTACC 2 cut(s) 965, 2426
AspLEI GCGC 3 cut(s) 11, 1170, 2455
AsuC2I CCSGG 4 cut(s) 238, 901, 2192, 2512
AsuHPI GGTGA 8 cut(s) 64, 516, 1406, 1410, 1460, 1867, 1905, 2395
AvaII GGWCC 7 cut(s) 338, 356, 506, 553, 727, 933, 2531
AxyI CCTNAGG 1 cut(s) 2382
BaeGI GKGCMC 1 cut(s) 1578
BalI TGGCCA 2 cut(s) 1379, 2421
BamHI GGATCC 2 cut(s) 127, 163
BanI GGYRCC 3 cut(s) 368, 965, 2426
BarI GAAGNNNNNNTAC 2 cut(s) 1167, 1199
BauI CACGAG 2 cut(s) 405, 704
BbrPI CACGTG 1 cut(s) 1631
BbsI GAAGAC 1 cut(s) 53
Bbv12I GWGCWC 2 cut(s) 60, 2215
BbvCI CCTCAGC 2 cut(s) 1164, 2034
BccI CCATC 8 cut(s) 431, 718, 777, 922, 1533, 1718, 2009, 2185
BceAI ACGGC 2 cut(s) 610, 1498
BciT130I CCWGG 8 cut(s) 249, 621, 732, 1650, 1752, 1869, 2334, 2535
BciVI GTATCC 2 cut(s) 2070, 2356
BclI TGATCA 1 cut(s) 1405
BcnI CCSGG 4 cut(s) 238, 901, 2192, 2512
BcoDI GTCTC 8 cut(s) 131, 238, 404, 1310, 1626, 1812, 2334, 2444
BcuI ACTAGT 1 cut(s) 467
BfaI CTAG 2 cut(s) 468, 1145
BfmI CTRYAG 6 cut(s) 228, 777, 1065, 1133, 1558, 1960
BfoI RGCGCY 1 cut(s) 12
BfuI GTATCC 2 cut(s) 2070, 2356
BglI GCCNNNNNGGC 1 cut(s) 1650
BmcAI AGTACT 2 cut(s) 466, 1264
Bme18I GGWCC 7 cut(s) 338, 356, 506, 553, 727, 933, 2531
BmrI ACTGGG 2 cut(s) 354, 554
BmsI GCATC 7 cut(s) 304, 487, 556, 671, 890, 1052, 1914
BmuI ACTGGG 2 cut(s) 354, 554
BpiI GAAGAC 1 cut(s) 53
BpmI CTGGAG 4 cut(s) 270, 616, 1539, 1980
Bpu10I CCTNAGC 5 cut(s) 17, 626, 1164, 1708, 2034
BpuEI CTTGAG 3 cut(s) 1342, 1407, 2028
BpuMI CCSGG 4 cut(s) 238, 901, 2192, 2512
BsaAI YACGTR 1 cut(s) 1631
BsaI GGTCTC 3 cut(s) 131, 238, 2444
BsaJI CCNNGG 8 cut(s) 730, 1751, 1868, 2190, 2422, 2505, 2511, 2534
BsaWI WCCGGW 1 cut(s) 526
BsaXI ACNNNNNCTCC 2 cut(s) 1849, 1879
Bse118I RCCGGY 5 cut(s) 74, 526, 930, 1838, 2048
Bse1I ACTGG 6 cut(s) 360, 560, 633, 1270, 1467, 2582
Bse21I CCTNAGG 1 cut(s) 2382
Bse3DI GCAATG 5 cut(s) 111, 604, 1508, 2026, 2548
BseBI CCWGG 8 cut(s) 249, 621, 732, 1650, 1752, 1869, 2334, 2535
BseDI CCNNGG 8 cut(s) 730, 1751, 1868, 2190, 2422, 2505, 2511, 2534
BseGI GGATG 8 cut(s) 478, 571, 772, 1408, 1463, 1929, 2001, 2479
BseMI GCAATG 5 cut(s) 111, 604, 1508, 2026, 2548
BseNI ACTGG 6 cut(s) 360, 560, 633, 1270, 1467, 2582
BseRI GAGGAG 3 cut(s) 104, 676, 2370
BseSI GKGCMC 1 cut(s) 1578
BseYI CCCAGC 5 cut(s) 11, 144, 658, 1678, 1919
BsgI GTGCAG 2 cut(s) 789, 1491
Bsh1285I CGRYCG 2 cut(s) 54, 280
BshNI GGYRCC 3 cut(s) 368, 965, 2426
BshTI ACCGGT 1 cut(s) 526
BsiEI CGRYCG 2 cut(s) 54, 280
BsiHKAI GWGCWC 2 cut(s) 60, 2215
BsiSI CCGG 9 cut(s) 75, 238, 527, 900, 931, 1839, 2049, 2192, 2511
BslFI GGGAC 9 cut(s) 220, 273, 539, 713, 919, 2140, 2307, 2482, 2544
BsmAI GTCTC 8 cut(s) 131, 238, 404, 1310, 1626, 1812, 2334, 2444
BsmBI CGTCTC 1 cut(s) 2334
BsmFI GGGAC 9 cut(s) 220, 273, 539, 713, 919, 2140, 2307, 2482, 2544
BsmI GAATGC 2 cut(s) 122, 853
Bso31I GGTCTC 3 cut(s) 131, 238, 2444
Bsp1286I GDGCHC 3 cut(s) 60, 1578, 2215
Bsp143I GATC 9 cut(s) 51, 69, 127, 163, 694, 1405, 1910, 2248, 2309
Bsp19I CCATGG 2 cut(s) 2422, 2505
BspHI TCATGA 1 cut(s) 172
BspMAI CTGCAG 3 cut(s) 232, 781, 1137
BspPI GGATC 7 cut(s) 122, 135, 158, 171, 689, 2256, 2317
BspT107I GGYRCC 3 cut(s) 368, 965, 2426
BspTNI GGTCTC 3 cut(s) 131, 238, 2444
BsrBI CCGCTC 2 cut(s) 1707, 2147
BsrDI GCAATG 5 cut(s) 111, 604, 1508, 2026, 2548
BsrFI RCCGGY 5 cut(s) 74, 526, 930, 1838, 2048
BsrI ACTGG 6 cut(s) 360, 560, 633, 1270, 1467, 2582
BssAI RCCGGY 5 cut(s) 74, 526, 930, 1838, 2048
BssECI CCNNGG 8 cut(s) 730, 1751, 1868, 2190, 2422, 2505, 2511, 2534
BssMI GATC 9 cut(s) 51, 69, 127, 163, 694, 1405, 1910, 2248, 2309
BssNAI GTATAC 1 cut(s) 2284
BssSI CACGAG 2 cut(s) 405, 704
BssT1I CCWWGG 2 cut(s) 2422, 2505
Bst1107I GTATAC 1 cut(s) 2284
Bst2BI CACGAG 2 cut(s) 405, 704
Bst2UI CCWGG 8 cut(s) 249, 621, 732, 1650, 1752, 1869, 2334, 2535
Bst4CI ACNGT 7 cut(s) 38, 86, 926, 1562, 1964, 2476, 2496
Bst6I CTCTTC 2 cut(s) 694, 877
BstAPI GCANNNNNTGC 1 cut(s) 290
BstBAI YACGTR 1 cut(s) 1631
BstC8I GCNNGC 5 cut(s) 823, 1599, 2415, 2419, 2457
BstDSI CCRYGG 2 cut(s) 2422, 2505
BstF5I GGATG 8 cut(s) 478, 571, 772, 1408, 1463, 1929, 2001, 2479
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 3 cut(s) 11, 1170, 2455
BstKTI GATC 9 cut(s) 54, 72, 130, 166, 697, 1408, 1913, 2251, 2312
BstMAI GTCTC 8 cut(s) 131, 238, 404, 1310, 1626, 1812, 2334, 2444
BstMBI GATC 9 cut(s) 51, 69, 127, 163, 694, 1405, 1910, 2248, 2309
BstMCI CGRYCG 2 cut(s) 54, 280
BstNI CCWGG 8 cut(s) 249, 621, 732, 1650, 1752, 1869, 2334, 2535
BstNSI RCATGY 3 cut(s) 1202, 2396, 2567
BstSFI CTRYAG 6 cut(s) 228, 777, 1065, 1133, 1558, 1960
BstSLI GKGCMC 1 cut(s) 1578
BstV2I GAAGAC 1 cut(s) 53
BstX2I RGATCY 4 cut(s) 127, 163, 694, 2248
BstYI RGATCY 4 cut(s) 127, 163, 694, 2248
BstZ17I GTATAC 1 cut(s) 2284
Bsu36I CCTNAGG 1 cut(s) 2382
BsuI GTATCC 2 cut(s) 2070, 2356
BtgI CCRYGG 2 cut(s) 2422, 2505
BtsCI GGATG 8 cut(s) 478, 571, 772, 1408, 1463, 1929, 2001, 2479
BtsI GCAGTG 2 cut(s) 1380, 1503
BtsIMutI CAGTG 7 cut(s) 43, 91, 567, 651, 1380, 1474, 1503
Cac8I GCNNGC 5 cut(s) 823, 1599, 2415, 2419, 2457
CaiI CAGNNNCTG 5 cut(s) 737, 1211, 1715, 2333, 2474
CciI TCATGA 1 cut(s) 172
CfoI GCGC 3 cut(s) 11, 1170, 2455
Cfr10I RCCGGY 5 cut(s) 74, 526, 930, 1838, 2048
Csp6I GTAC 6 cut(s) 465, 744, 966, 1263, 1768, 2427
CspAI ACCGGT 1 cut(s) 526
CviQI GTAC 6 cut(s) 465, 744, 966, 1263, 1768, 2427
DpnI GATC 9 cut(s) 53, 71, 129, 165, 696, 1407, 1912, 2250, 2311
DpnII GATC 9 cut(s) 51, 69, 127, 163, 694, 1405, 1910, 2248, 2309
EaeI YGGCCR 6 cut(s) 1377, 1726, 2335, 2419, 2508, 2579
Eam1104I CTCTTC 2 cut(s) 694, 877
EarI CTCTTC 2 cut(s) 694, 877
Eco130I CCWWGG 2 cut(s) 2422, 2505
Eco31I GGTCTC 3 cut(s) 131, 238, 2444
Eco47I GGWCC 7 cut(s) 338, 356, 506, 553, 727, 933, 2531
Eco57I CTGAAG 2 cut(s) 675, 768
Eco72I CACGTG 1 cut(s) 1631
Eco81I CCTNAGG 1 cut(s) 2382
EcoO109I RGGNCCY 3 cut(s) 727, 1072, 2531
EcoRII CCWGG 8 cut(s) 247, 619, 730, 1648, 1750, 1867, 2332, 2533
EcoT14I CCWWGG 2 cut(s) 2422, 2505
EcoT22I ATGCAT 1 cut(s) 1375
ErhI CCWWGG 2 cut(s) 2422, 2505
Esp3I CGTCTC 1 cut(s) 2334
FaqI GGGAC 9 cut(s) 220, 273, 539, 713, 919, 2140, 2307, 2482, 2544
FauI CCCGC 2 cut(s) 1649, 1814
FauNDI CATATG 2 cut(s) 303, 2151
FbaI TGATCA 1 cut(s) 1405
FblI GTMKAC 1 cut(s) 2283
FokI GGATG 8 cut(s) 465, 578, 759, 1395, 1450, 1936, 1988, 2466
FspBI CTAG 2 cut(s) 468, 1145
FspI TGCGCA 1 cut(s) 2454
GlaI GCGC 3 cut(s) 10, 1169, 2454
GsaI CCCAGC 5 cut(s) 15, 148, 662, 1682, 1923
GsuI CTGGAG 4 cut(s) 270, 616, 1539, 1980
HaeII RGCGCY 1 cut(s) 12
HapII CCGG 9 cut(s) 75, 238, 527, 900, 931, 1839, 2049, 2192, 2511
HhaI GCGC 3 cut(s) 11, 1170, 2455
Hin6I GCGC 3 cut(s) 9, 1168, 2453
HinP1I GCGC 3 cut(s) 9, 1168, 2453
HincII GTYRAC 1 cut(s) 2521
HindII GTYRAC 1 cut(s) 2521
HpaII CCGG 9 cut(s) 75, 238, 527, 900, 931, 1839, 2049, 2192, 2511
HphI GGTGA 8 cut(s) 64, 516, 1406, 1410, 1460, 1867, 1905, 2395
Hpy166II GTNNAC 2 cut(s) 2284, 2521
Hpy188III TCNNGA 9 cut(s) 161, 173, 407, 509, 1098, 1205, 1283, 1403, 1518
Hpy8I GTNNAC 2 cut(s) 2284, 2521
HpyAV CCTTC 6 cut(s) 1086, 1169, 1385, 1655, 2300, 2384
HpyCH4III ACNGT 7 cut(s) 38, 86, 926, 1562, 1964, 2476, 2496
HpyCH4IV ACGT 3 cut(s) 190, 1630, 2343
HpySE526I ACGT 3 cut(s) 190, 1630, 2343
HspAI GCGC 3 cut(s) 9, 1168, 2453
KflI GGGWCCC 1 cut(s) 727
KpnI GGTACC 2 cut(s) 969, 2430
Ksp22I TGATCA 1 cut(s) 1405
Kzo9I GATC 9 cut(s) 51, 69, 127, 163, 694, 1405, 1910, 2248, 2309
LmnI GCTCC 6 cut(s) 635, 765, 1042, 1870, 2071, 2218
LweI GCATC 7 cut(s) 304, 487, 556, 671, 890, 1052, 1914
MaeI CTAG 2 cut(s) 468, 1145
MaeII ACGT 3 cut(s) 190, 1630, 2343
MaeIII GTNAC 8 cut(s) 191, 522, 1083, 1855, 1964, 2395, 2461, 2585
MalI GATC 9 cut(s) 53, 71, 129, 165, 696, 1407, 1912, 2250, 2311
MbiI CCGCTC 2 cut(s) 1707, 2147
MboI GATC 9 cut(s) 51, 69, 127, 163, 694, 1405, 1910, 2248, 2309
MboII GAAGA 6 cut(s) 58, 681, 864, 1127, 1175, 1346
MfeI CAATTG 1 cut(s) 941
MflI RGATCY 4 cut(s) 127, 163, 694, 2248
MhlI GDGCHC 3 cut(s) 60, 1578, 2215
MlsI TGGCCA 2 cut(s) 1379, 2421
MluNI TGGCCA 2 cut(s) 1379, 2421
MlyI GAGTC 5 cut(s) 242, 913, 1110, 1600, 1628
MmeI TCCRAC 1 cut(s) 2515
Mox20I TGGCCA 2 cut(s) 1379, 2421
Mph1103I ATGCAT 1 cut(s) 1375
MscI TGGCCA 2 cut(s) 1379, 2421
MseI TTAA 4 cut(s) 588, 750, 2097, 2354
MslI CAYNNNNRTG 1 cut(s) 1197
Msp20I TGGCCA 2 cut(s) 1379, 2421
MspA1I CMGCKG 3 cut(s) 1028, 1090, 1919
MspI CCGG 9 cut(s) 75, 238, 527, 900, 931, 1839, 2049, 2192, 2511
MunI CAATTG 1 cut(s) 941
Mva1269I GAATGC 2 cut(s) 122, 853
MvaI CCWGG 8 cut(s) 249, 621, 732, 1650, 1752, 1869, 2334, 2535
NciI CCSGG 4 cut(s) 238, 901, 2192, 2512
NcoI CCATGG 2 cut(s) 2422, 2505
NdeI CATATG 2 cut(s) 303, 2151
NdeII GATC 9 cut(s) 51, 69, 127, 163, 694, 1405, 1910, 2248, 2309
NmeAIII GCCGAG 2 cut(s) 397, 2363
NmuCI GTSAC 6 cut(s) 191, 522, 1083, 1855, 1964, 2395
NsbI TGCGCA 1 cut(s) 2454
NsiI ATGCAT 1 cut(s) 1375
NspI RCATGY 3 cut(s) 1202, 2396, 2567
PagI TCATGA 1 cut(s) 172
PciI ACATGT 1 cut(s) 1198
PctI GAATGC 2 cut(s) 122, 853
PfeI GAWTC 8 cut(s) 208, 224, 719, 1061, 1109, 1208, 1337, 1490
PflFI GACNNNGTC 1 cut(s) 360
PflMI CCANNNNNTGG 2 cut(s) 2014, 2506
PfoI TCCNGGA 1 cut(s) 899
PinAI ACCGGT 1 cut(s) 526
Ple19I CGATCG 1 cut(s) 54
PleI GAGTC 5 cut(s) 241, 912, 1109, 1599, 1627
PmaCI CACGTG 1 cut(s) 1631
PmlI CACGTG 1 cut(s) 1631
PpsI GAGTC 5 cut(s) 241, 912, 1109, 1599, 1627
Ppu21I YACGTR 1 cut(s) 1631
PpuMI RGGWCCY 2 cut(s) 727, 2531
PscI ACATGT 1 cut(s) 1198
PsiI TTATAA 1 cut(s) 2202
Psp5II RGGWCCY 2 cut(s) 727, 2531
Psp6I CCWGG 8 cut(s) 247, 619, 730, 1648, 1750, 1867, 2332, 2533
PspCI CACGTG 1 cut(s) 1631
PspFI CCCAGC 5 cut(s) 11, 144, 658, 1678, 1919
PspGI CCWGG 8 cut(s) 247, 619, 730, 1648, 1750, 1867, 2332, 2533
PspPPI RGGWCCY 2 cut(s) 727, 2531
PstI CTGCAG 3 cut(s) 232, 781, 1137
PstNI CAGNNNCTG 5 cut(s) 737, 1211, 1715, 2333, 2474
PsuI RGATCY 4 cut(s) 127, 163, 694, 2248
PsyI GACNNNGTC 1 cut(s) 360
PvuI CGATCG 1 cut(s) 54
PvuII CAGCTG 3 cut(s) 1028, 1090, 1919
RsaI GTAC 6 cut(s) 466, 745, 967, 1264, 1769, 2428
RsaNI GTAC 6 cut(s) 465, 744, 966, 1263, 1768, 2427
RseI CAYNNNNRTG 1 cut(s) 1197
SaqAI TTAA 4 cut(s) 588, 750, 2097, 2354
Sau3AI GATC 9 cut(s) 51, 69, 127, 163, 694, 1405, 1910, 2248, 2309
ScaI AGTACT 2 cut(s) 466, 1264
SchI GAGTC 5 cut(s) 242, 913, 1110, 1600, 1628
SduI GDGCHC 3 cut(s) 60, 1578, 2215
SfaNI GCATC 7 cut(s) 304, 487, 556, 671, 890, 1052, 1914
SfcI CTRYAG 6 cut(s) 228, 777, 1065, 1133, 1558, 1960
SinI GGWCC 7 cut(s) 338, 356, 506, 553, 727, 933, 2531
SmiMI CAYNNNNRTG 1 cut(s) 1197
SmlI CTYRAG 3 cut(s) 1357, 1422, 2043
SmoI CTYRAG 3 cut(s) 1357, 1422, 2043
SpeI ACTAGT 1 cut(s) 467
SspI AATATT 2 cut(s) 1700, 2012
SspMI CTAG 2 cut(s) 468, 1145
StyI CCWWGG 2 cut(s) 2422, 2505
TaaI ACNGT 7 cut(s) 38, 86, 926, 1562, 1964, 2476, 2496
TaiI ACGT 3 cut(s) 193, 1633, 2346
TaqI TCGA 3 cut(s) 54, 435, 1909
TaqII GACCGA 1 cut(s) 541
TatI WGTACW 3 cut(s) 464, 1262, 1767
TauI GCSGC 3 cut(s) 1478, 1731, 2090
TfiI GAWTC 8 cut(s) 208, 224, 719, 1061, 1109, 1208, 1337, 1490
Tru1I TTAA 4 cut(s) 588, 750, 2097, 2354
Tru9I TTAA 4 cut(s) 588, 750, 2097, 2354
TscAI CASTG 7 cut(s) 43, 91, 567, 651, 1387, 1474, 1510
TseFI GTSAC 6 cut(s) 191, 522, 1083, 1855, 1964, 2395
Tsp45I GTSAC 6 cut(s) 191, 522, 1083, 1855, 1964, 2395
TspDTI ATGAA 7 cut(s) 161, 711, 864, 1404, 1503, 2468, 2588
TspGWI ACGGA 1 cut(s) 2058
TspRI CASTG 7 cut(s) 43, 91, 567, 651, 1387, 1474, 1510
Tth111I GACNNNGTC 1 cut(s) 360
Van91I CCANNNNNTGG 2 cut(s) 2014, 2506
VpaK11BI GGWCC 7 cut(s) 338, 356, 506, 553, 727, 933, 2531
XapI RAATTY 2 cut(s) 286, 331
XceI RCATGY 3 cut(s) 1202, 2396, 2567
XcmI CCANNNNNNNNNTGG 2 cut(s) 494, 944
XmiI GTMKAC 1 cut(s) 2283
XspI CTAG 2 cut(s) 468, 1145
ZrmI AGTACT 2 cut(s) 466, 1264
Zsp2I ATGCAT 1 cut(s) 1375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.