MD17G1118800.v1.1

Protein of unknown function (DUF1296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0020160
Physical Location & Seq
Forward (+)
10284393 .. 10292797
8405 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1118800.v1.1.491

Sequence Viewer

Length: 2598 bp
ATGAGTGGCGGCGGGCTTAGGGTTTCAATCCCCAACGGTGTGAGGAAGACGATCCAGGACATCAAGGAGATCACCGGCAATCACAGTGAGGAGGAAATCCACGCAATGCTCAAAGAATGCAACATGGATCCTAACGAGACCGCCCAGAAGCTTCTCCTTCAGGATCCATTCCATGAGGTTAAAAGGAAACGCGACAAGAGAAAAGAGAATCTGAACAACAAAGAGTCTGCAGAATCCCGGTGGAGACCTGGAGGGCAGGGACGGGGGGGTAGGGGTGGCCGGGCGAATTTTGCACCTCGTCATATGCCACGTGATGCTGGTGGTGGCAGAAATGCTGGTCCTGCAACTGAGAATGGACCCAGTCAGGGTGCCGAGAAAGGTGGTTCTCCCTCTTTCCCACCCTCTCGTGAGACAAAAAATAAAGAGAGAAGTGTGGATGGGCCCACCAATGTGGCTTCTGGAGGTCCTAGTGTGGTGCATCCCCCCCATTTATCTGCTGGAAGTGGTCCTGAAATAAGTTTGTCACGGGTTGGGGATAATTTGGGAAGTTTGTTCCCCCCAGTGAATGCAAAAGAAAGTCCCAGCATTAAATTTGGCAATGAGGACTTGCAGGAGCAGCCCGCACCAAGCTCCAGTAGCAGCTCAGTGTTGGCAAACCCAGCATCTACACTGGCAGCTTGTTTCTCACCTTCAGATCCTGTACTCATGCCATCTAATGATTCACGGCTCCCAGGTTCTGTGGGCACAATTAAACGTGAAGTAGGGAGCCATCATCCTCCAGCTTCAGAGGTTGGTAGCTCTCCTGCACAAGGTAAAGTGGCAAGCAAGACACAGGGAGTTGGGAAAAGTCAGCTTTCTGATCTTTCACGCTCTTCATCTACATCAACTCATGGTGGTTCAGGTAGTCGACCATCATCTAATTACAGTAGCCGGTCACAACAATTGATTGGCACTCAAAAAGTTGGTACCAACAAAGAGTGGAAACCAAAACCAATCAATTCTGCTGTTGTTCAAGGTCTGGGAACTGCTCCCACATCTGAGCTTTCTTCCAATTCTATAGAGGTCCCTTCCCAGTCACAGCTGGTCTCAAGAGTCCTTGATTCAGAAGAAGCAACTTCAAAACTGCAGAGAAAGCTAGAGGAATTACACCTTCCTCAGCGCAAAGTAGTTATTCTTCCAAATCACATCCATGTTCCAGAATCTGAAAGAACCAAGTTGAGTTTTGGAAGTTTTGGTGCTATGTTTGGCATAAGTACTGGCTATGTGAGTGCTCCTGAGAGTGATAAGAGCTCAACACCACGGTCCGAGACTTCTCAGGTTGTTGAAGAATCTGTGGAGGAACAATCCTCAAGCAATCAAAATGCTTCGGCAACTGCTGATGAAGGTGATTATCCTGATCATCCGCAATCACCTCCTCTAGTATCCGAAAATATATCATCTGGTGAGGGTGATGTATCATCCAGTGTATCCCCAGGACACAATGAATCTAAGCATGACACTGCATTGCCATCTGGGGGCCATCAGTTCTCGGTGGCTCATACTTCTCCGAACTACAGTTTTGGGTTTGTGCCCCCAATTTTAGGGAGTCAGCTTGCACCATTTGAAAACTCTGAGTCTCAAGCACGTGATGTTTCTCGCCTTCCAAGCTTTGTAGTACAACCACCCTTTGACCCAGCAAGTTATTATGCCCAATATTACCGCTCAGGTGCTGATAGTGATGGCCGCCTTTCTCCATTTCCTTCTCCTGGGGTTTCCACCAAGTACAATGCGAACGTTGCAGTATTGCCTCAAAGTTCTCAGTCTCCCCAAGAGGGCGGGGTTCTATCTGCAGCTGGCCCAACACCATTGGTGACACAGGCTCCTGGGATTGTTCAGAGCTCGATATCTGTAACTCAGCAGCCAGTTCCTGTCTTTCGACCACCAGCTGGGATGCACATATCTCATTATCCTCATAACTATTTTCCCTACAGTCACTACTTTTCCCCAATCTATGTTCCACCTCCAGCCATCCACCAATTTTTGGGCAATGGTGCATTTCCTCAGCAACCTCAAGCTGGTGGTGTGTATCCAGCTCCGCCAGCAGCAGCAGCCACAGGAGTCAAATACTCACTGCCGCAATACAAAACAGGGACTAATACAGGGAACTCTGCCCATATGGGAATGGCAAGTGGTTATGGGCCATATGGCTCTTCCCCAGCTGGTTATAATCCGAGTGCTCCCACAACGGTAGGAAACTCTACGTCTAATGAGGATCTTGCCTCATCTCAGTTCAAGGAAAATAATGCATACATCACGGGACAGCAGAGTGAAGGATCTTCTGTGTGGGTTGCAGCACCTGGCCGAGACATATCCAGCTTAACAAGTTCATTTTACAACCTTCCCCCTCAGGGTCAGCACATGACTTTCACCCCAACGCAGGCTGGCCATGGAACCTTTGCTGGTCTCTACCACCCTGCACAAGCAGTAACAGCAGCAGCAGTTCATCCGCTTCTCCAACAACCCCAGACCATGGCTGGGGCTGTTGACATGGTGGGACCTGGGGGCAATGTTTATCAACAGCCTCAACATGCACAAATGAACTGGCCGAGTAACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1296

Protein Analysis

866

Amino Acids

90.91

Weight (kDa)

6.56

Isoelectric Point (pI)

69.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIP1_N PF06972 10 - 69 2.1e-33 GBF-interacting protein 1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020160)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07660
malus_domestica MD09G1129800.v1.1 MD17G1118800.v1.1
prunus_persica Prupe.3G195400_v2.0.a1
pyrus_communis pycom09g05290 pycom17g10910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2205
Acc65I GGTACC 1 cut(s) 965
AccB1I GGYRCC 2 cut(s) 368, 965
AccB7I CCANNNNNTGG 3 cut(s) 1925, 2020, 2509
AccBSI CCGCTC 1 cut(s) 1701
AccI GTMKAC 1 cut(s) 907
AccII CGCG 1 cut(s) 192
AclI AACGTT 1 cut(s) 1773
AclWI GGATC 8 cut(s) 46, 122, 135, 158, 171, 689, 2259, 2320
AcoI YGGCCR 5 cut(s) 277, 1720, 2338, 2422, 2582
AcsI RAATTY 2 cut(s) 286, 590
AcuI CTGAAG 3 cut(s) 143, 675, 768
AcvI CACGTG 2 cut(s) 311, 1625
AfaI GTAC 5 cut(s) 702, 967, 1255, 1656, 1763
AgsI TTSAA 6 cut(s) 27, 1013, 1119, 1325, 1604, 2272
AjnI CCWGG 8 cut(s) 54, 247, 730, 1471, 1744, 1861, 2335, 2536
AleI CACNNNNGTG 1 cut(s) 449
Alw21I GWGCWC 4 cut(s) 1273, 1292, 1880, 2218
Alw26I GTCTC 9 cut(s) 131, 238, 404, 1090, 1301, 1620, 1806, 2337, 2447
AlwI GGATC 8 cut(s) 46, 122, 135, 158, 171, 689, 2259, 2320
AlwNI CAGNNNCTG 6 cut(s) 698, 737, 1202, 1709, 1907, 2336
AoxI GGCC 9 cut(s) 277, 440, 1516, 1720, 1834, 2177, 2338, 2422, 2582
ApaI GGGCCC 1 cut(s) 444
ApoI RAATTY 2 cut(s) 286, 590
Asp718I GGTACC 1 cut(s) 965
AspLEI GCGC 1 cut(s) 1161
AsuC2I CCSGG 2 cut(s) 238, 281
AsuHPI GGTGA 8 cut(s) 64, 678, 1397, 1401, 1454, 1460, 1861, 2398
AvaII GGWCC 7 cut(s) 338, 356, 464, 506, 1063, 1302, 2534
AxyI CCTNAGG 1 cut(s) 2385
BaeGI GKGCMC 3 cut(s) 444, 746, 1572
BalI TGGCCA 1 cut(s) 2424
BamHI GGATCC 2 cut(s) 127, 163
BanI GGYRCC 2 cut(s) 368, 965
BanII GRGCYC 3 cut(s) 444, 1292, 1880
BarI GAAGNNNNNNTAC 2 cut(s) 1158, 1190
BauI CACGAG 1 cut(s) 405
BbrPI CACGTG 2 cut(s) 311, 1625
BbsI GAAGAC 1 cut(s) 53
Bbv12I GWGCWC 4 cut(s) 1273, 1292, 1880, 2218
BbvCI CCTCAGC 2 cut(s) 1155, 2040
BccI CCATC 8 cut(s) 431, 718, 777, 919, 1516, 1527, 1712, 2015
BceAI ACGGC 1 cut(s) 740
BciT130I CCWGG 8 cut(s) 56, 249, 732, 1473, 1746, 1863, 2337, 2538
BciVI GTATCC 3 cut(s) 1432, 1477, 2076
BclI TGATCA 1 cut(s) 1396
BcnI CCSGG 2 cut(s) 238, 281
BcoDI GTCTC 9 cut(s) 131, 238, 404, 1090, 1301, 1620, 1806, 2337, 2447
BfaI CTAG 3 cut(s) 468, 1136, 1418
BfmI CTRYAG 6 cut(s) 228, 1056, 1124, 1552, 1827, 1966
BfuI GTATCC 3 cut(s) 1432, 1477, 2076
BmcAI AGTACT 1 cut(s) 1255
Bme18I GGWCC 7 cut(s) 338, 356, 464, 506, 1063, 1302, 2534
BmrI ACTGGG 3 cut(s) 354, 554, 1066
BmsI GCATC 4 cut(s) 304, 487, 671, 1920
BmuI ACTGGG 3 cut(s) 354, 554, 1066
BpiI GAAGAC 1 cut(s) 53
BpmI CTGGAG 5 cut(s) 270, 480, 616, 762, 1986
Bpu10I CCTNAGC 4 cut(s) 17, 1155, 1702, 2040
BpuEI CTTGAG 4 cut(s) 1072, 1333, 1602, 2034
BpuMI CCSGG 2 cut(s) 238, 281
BsaAI YACGTR 2 cut(s) 311, 1625
BsaI GGTCTC 4 cut(s) 131, 238, 1090, 2447
BsaJI CCNNGG 8 cut(s) 730, 1298, 1471, 1745, 1862, 2425, 2508, 2537
BsaXI ACNNNNNCTCC 2 cut(s) 1843, 1873
Bse118I RCCGGY 2 cut(s) 74, 930
Bse1I ACTGG 9 cut(s) 360, 560, 633, 675, 1072, 1261, 1461, 1901, 2585
Bse21I CCTNAGG 1 cut(s) 2385
Bse3DI GCAATG 5 cut(s) 111, 604, 1502, 2032, 2551
BseBI CCWGG 8 cut(s) 56, 249, 732, 1473, 1746, 1863, 2337, 2538
BseDI CCNNGG 8 cut(s) 730, 1298, 1471, 1745, 1862, 2425, 2508, 2537
BseGI GGATG 9 cut(s) 442, 478, 772, 1185, 1399, 1457, 1935, 2007, 2482
BseMI GCAATG 5 cut(s) 111, 604, 1502, 2032, 2551
BseNI ACTGG 9 cut(s) 360, 560, 633, 675, 1072, 1261, 1461, 1901, 2585
BseRI GAGGAG 2 cut(s) 104, 1404
BseSI GKGCMC 3 cut(s) 444, 746, 1572
BseYI CCCAGC 6 cut(s) 581, 658, 1672, 1925, 2194, 2513
BsgI GTGCAG 2 cut(s) 789, 2439
Bsh1236I CGCG 1 cut(s) 192
BshFI GGCC 9 cut(s) 279, 442, 1518, 1722, 1836, 2179, 2340, 2424, 2584
BshNI GGYRCC 2 cut(s) 368, 965
BsiHKAI GWGCWC 4 cut(s) 1273, 1292, 1880, 2218
BsiSI CCGG 4 cut(s) 75, 238, 280, 931
BslFI GGGAC 6 cut(s) 273, 564, 1049, 2143, 2310, 2547
BsmAI GTCTC 9 cut(s) 131, 238, 404, 1090, 1301, 1620, 1806, 2337, 2447
BsmFI GGGAC 6 cut(s) 273, 564, 1049, 2143, 2310, 2547
BsmI GAATGC 2 cut(s) 122, 571
BsnI GGCC 9 cut(s) 279, 442, 1518, 1722, 1836, 2179, 2340, 2424, 2584
Bso31I GGTCTC 4 cut(s) 131, 238, 1090, 2447
Bsp120I GGGCCC 1 cut(s) 440
Bsp1286I GDGCHC 7 cut(s) 444, 746, 1273, 1292, 1572, 1880, 2218
Bsp143I GATC 9 cut(s) 51, 69, 127, 163, 694, 859, 1396, 2251, 2312
Bsp19I CCATGG 2 cut(s) 2425, 2508
BspANI GGCC 9 cut(s) 279, 442, 1518, 1722, 1836, 2179, 2340, 2424, 2584
BspFNI CGCG 1 cut(s) 192
BspMAI CTGCAG 3 cut(s) 232, 1128, 1831
BspPI GGATC 8 cut(s) 46, 122, 135, 158, 171, 689, 2259, 2320
BspQI GCTCTTC 2 cut(s) 877, 2194
BspT107I GGYRCC 2 cut(s) 368, 965
BspTNI GGTCTC 4 cut(s) 131, 238, 1090, 2447
BsrBI CCGCTC 1 cut(s) 1701
BsrDI GCAATG 5 cut(s) 111, 604, 1502, 2032, 2551
BsrFI RCCGGY 2 cut(s) 74, 930
BsrI ACTGG 9 cut(s) 360, 560, 633, 675, 1072, 1261, 1461, 1901, 2585
BssAI RCCGGY 2 cut(s) 74, 930
BssECI CCNNGG 8 cut(s) 730, 1298, 1471, 1745, 1862, 2425, 2508, 2537
BssMI GATC 9 cut(s) 51, 69, 127, 163, 694, 859, 1396, 2251, 2312
BssSI CACGAG 1 cut(s) 405
BssT1I CCWWGG 2 cut(s) 2425, 2508
Bst2BI CACGAG 1 cut(s) 405
Bst2UI CCWGG 8 cut(s) 56, 249, 732, 1473, 1746, 1863, 2337, 2538
Bst4CI ACNGT 7 cut(s) 38, 86, 926, 1302, 1556, 1970, 2227
Bst6I CTCTTC 2 cut(s) 877, 2194
BstBAI YACGTR 2 cut(s) 311, 1625
BstC8I GCNNGC 8 cut(s) 14, 621, 823, 1593, 1834, 2079, 2418, 2422
BstDSI CCRYGG 3 cut(s) 1298, 2425, 2508
BstENI CCTNNNNNAGG 1 cut(s) 807
BstF5I GGATG 9 cut(s) 442, 478, 772, 1185, 1399, 1457, 1935, 2007, 2482
BstFNI CGCG 1 cut(s) 192
BstHHI GCGC 1 cut(s) 1161
BstKTI GATC 9 cut(s) 54, 72, 130, 166, 697, 862, 1399, 2254, 2315
BstMAI GTCTC 9 cut(s) 131, 238, 404, 1090, 1301, 1620, 1806, 2337, 2447
BstMBI GATC 9 cut(s) 51, 69, 127, 163, 694, 859, 1396, 2251, 2312
BstNI CCWGG 8 cut(s) 56, 249, 732, 1473, 1746, 1863, 2337, 2538
BstNSI RCATGY 1 cut(s) 2570
BstSFI CTRYAG 6 cut(s) 228, 1056, 1124, 1552, 1827, 1966
BstSLI GKGCMC 3 cut(s) 444, 746, 1572
BstUI CGCG 1 cut(s) 192
BstV2I GAAGAC 1 cut(s) 53
BstX2I RGATCY 5 cut(s) 127, 163, 694, 2251, 2312
BstXI CCANNNNNNTGG 1 cut(s) 451
BstYI RGATCY 5 cut(s) 127, 163, 694, 2251, 2312
Bsu36I CCTNAGG 1 cut(s) 2385
BsuI GTATCC 3 cut(s) 1432, 1477, 2076
BsuRI GGCC 9 cut(s) 279, 442, 1518, 1722, 1836, 2179, 2340, 2424, 2584
BtgI CCRYGG 3 cut(s) 1298, 2425, 2508
BtsCI GGATG 9 cut(s) 442, 478, 772, 1185, 1399, 1457, 1935, 2007, 2482
BtsI GCAGTG 2 cut(s) 1497, 2108
BtsIMutI CAGTG 7 cut(s) 91, 567, 651, 668, 1468, 1497, 2108
Cac8I GCNNGC 8 cut(s) 14, 621, 823, 1593, 1834, 2079, 2418, 2422
CaiI CAGNNNCTG 6 cut(s) 698, 737, 1202, 1709, 1907, 2336
CfoI GCGC 1 cut(s) 1161
Cfr10I RCCGGY 2 cut(s) 74, 930
CpoI CGGWCCG 1 cut(s) 1302
Csp6I GTAC 5 cut(s) 701, 966, 1254, 1655, 1762
CspI CGGWCCG 1 cut(s) 1302
CviQI GTAC 5 cut(s) 701, 966, 1254, 1655, 1762
DpnI GATC 9 cut(s) 53, 71, 129, 165, 696, 861, 1398, 2253, 2314
DpnII GATC 9 cut(s) 51, 69, 127, 163, 694, 859, 1396, 2251, 2312
EaeI YGGCCR 5 cut(s) 277, 1720, 2338, 2422, 2582
Eam1104I CTCTTC 2 cut(s) 877, 2194
EarI CTCTTC 2 cut(s) 877, 2194
EciI GGCGGA 1 cut(s) 2064
Ecl136II GAGCTC 2 cut(s) 1290, 1878
Eco130I CCWWGG 2 cut(s) 2425, 2508
Eco24I GRGCYC 3 cut(s) 444, 1292, 1880
Eco31I GGTCTC 4 cut(s) 131, 238, 1090, 2447
Eco32I GATATC 1 cut(s) 1884
Eco47I GGWCC 7 cut(s) 338, 356, 464, 506, 1063, 1302, 2534
Eco53kI GAGCTC 2 cut(s) 1290, 1878
Eco57I CTGAAG 3 cut(s) 143, 675, 768
Eco72I CACGTG 2 cut(s) 311, 1625
Eco81I CCTNAGG 1 cut(s) 2385
EcoICRI GAGCTC 2 cut(s) 1290, 1878
EcoNI CCTNNNNNAGG 1 cut(s) 807
EcoO109I RGGNCCY 3 cut(s) 464, 1063, 2534
EcoRII CCWGG 8 cut(s) 54, 247, 730, 1471, 1744, 1861, 2335, 2536
EcoRV GATATC 1 cut(s) 1884
EcoT14I CCWWGG 2 cut(s) 2425, 2508
EcoT22I ATGCAT 1 cut(s) 2287
EcoT38I GRGCYC 3 cut(s) 444, 1292, 1880
ErhI CCWWGG 2 cut(s) 2425, 2508
FaqI GGGAC 6 cut(s) 273, 564, 1049, 2143, 2310, 2547
FauI CCCGC 3 cut(s) 5, 628, 1808
FauNDI CATATG 3 cut(s) 303, 2154, 2182
FbaI TGATCA 1 cut(s) 1396
FblI GTMKAC 1 cut(s) 907
FokI GGATG 9 cut(s) 449, 465, 759, 1172, 1386, 1444, 1942, 1994, 2469
FriOI GRGCYC 3 cut(s) 444, 1292, 1880
FspBI CTAG 3 cut(s) 468, 1136, 1418
GlaI GCGC 1 cut(s) 1160
GsaI CCCAGC 6 cut(s) 585, 662, 1676, 1929, 2198, 2517
GsuI CTGGAG 5 cut(s) 270, 480, 616, 762, 1986
HaeIII GGCC 9 cut(s) 279, 442, 1518, 1722, 1836, 2179, 2340, 2424, 2584
HapII CCGG 4 cut(s) 75, 238, 280, 931
HhaI GCGC 1 cut(s) 1161
Hin6I GCGC 1 cut(s) 1159
HinP1I GCGC 1 cut(s) 1159
HincII GTYRAC 2 cut(s) 908, 2524
HindII GTYRAC 2 cut(s) 908, 2524
HindIII AAGCTT 2 cut(s) 149, 1645
HpaII CCGG 4 cut(s) 75, 238, 280, 931
HphI GGTGA 8 cut(s) 64, 678, 1397, 1401, 1454, 1460, 1861, 2398
Hpy166II GTNNAC 2 cut(s) 908, 2524
Hpy188III TCNNGA 8 cut(s) 161, 407, 459, 509, 1089, 1196, 1274, 1394
Hpy8I GTNNAC 2 cut(s) 908, 2524
HpyAV CCTTC 9 cut(s) 167, 699, 1077, 1160, 1376, 1649, 1749, 2303, 2387
HpyCH4III ACNGT 7 cut(s) 38, 86, 926, 1302, 1556, 1970, 2227
HpyCH4IV ACGT 5 cut(s) 310, 754, 1624, 1773, 2240
HpySE526I ACGT 5 cut(s) 310, 754, 1624, 1773, 2240
HspAI GCGC 1 cut(s) 1159
KpnI GGTACC 1 cut(s) 969
Ksp22I TGATCA 1 cut(s) 1396
Kzo9I GATC 9 cut(s) 51, 69, 127, 163, 694, 859, 1396, 2251, 2312
LguI GCTCTTC 2 cut(s) 877, 2194
LmnI GCTCC 9 cut(s) 613, 635, 732, 765, 1033, 1276, 1864, 2077, 2221
LweI GCATC 4 cut(s) 304, 487, 671, 1920
MaeI CTAG 3 cut(s) 468, 1136, 1418
MaeII ACGT 5 cut(s) 310, 754, 1624, 1773, 2240
MaeIII GTNAC 8 cut(s) 522, 933, 1074, 1849, 1888, 1970, 2464, 2588
MalI GATC 9 cut(s) 53, 71, 129, 165, 696, 861, 1398, 2253, 2314
MbiI CCGCTC 1 cut(s) 1701
MboI GATC 9 cut(s) 51, 69, 127, 163, 694, 859, 1396, 2251, 2312
MboII GAAGA 8 cut(s) 58, 864, 1038, 1118, 1166, 1337, 2181, 2307
MfeI CAATTG 1 cut(s) 941
MflI RGATCY 5 cut(s) 127, 163, 694, 2251, 2312
MhlI GDGCHC 7 cut(s) 444, 746, 1273, 1292, 1572, 1880, 2218
MlsI TGGCCA 1 cut(s) 2424
MluNI TGGCCA 1 cut(s) 2424
MlyI GAGTC 5 cut(s) 233, 1101, 1594, 1622, 2106
MmeI TCCRAC 1 cut(s) 2518
Mox20I TGGCCA 1 cut(s) 2424
Mph1103I ATGCAT 1 cut(s) 2287
MscI TGGCCA 1 cut(s) 2424
MseI TTAA 4 cut(s) 180, 588, 750, 2357
MslI CAYNNNNRTG 2 cut(s) 449, 1188
Msp20I TGGCCA 1 cut(s) 2424
MspA1I CMGCKG 4 cut(s) 1081, 1832, 1925, 2198
MspI CCGG 4 cut(s) 75, 238, 280, 931
MunI CAATTG 1 cut(s) 941
Mva1269I GAATGC 2 cut(s) 122, 571
MvaI CCWGG 8 cut(s) 56, 249, 732, 1473, 1746, 1863, 2337, 2538
MvnI CGCG 1 cut(s) 192
NciI CCSGG 2 cut(s) 238, 281
NcoI CCATGG 2 cut(s) 2425, 2508
NdeI CATATG 3 cut(s) 303, 2154, 2182
NdeII GATC 9 cut(s) 51, 69, 127, 163, 694, 859, 1396, 2251, 2312
NmeAIII GCCGAG 2 cut(s) 397, 2366
NmuCI GTSAC 5 cut(s) 522, 933, 1074, 1849, 1970
NsiI ATGCAT 1 cut(s) 2287
NspI RCATGY 1 cut(s) 2570
OliI CACNNNNGTG 1 cut(s) 449
PciSI GCTCTTC 2 cut(s) 877, 2194
PctI GAATGC 2 cut(s) 122, 571
PfeI GAWTC 7 cut(s) 208, 233, 719, 1100, 1199, 1328, 1484
PflFI GACNNNGTC 1 cut(s) 360
PflMI CCANNNNNTGG 3 cut(s) 1925, 2020, 2509
PfoI TCCNGGA 1 cut(s) 54
PleI GAGTC 5 cut(s) 232, 1100, 1593, 1621, 2105
PmaCI CACGTG 2 cut(s) 311, 1625
PmlI CACGTG 2 cut(s) 311, 1625
PpsI GAGTC 5 cut(s) 232, 1100, 1593, 1621, 2105
Ppu21I YACGTR 2 cut(s) 311, 1625
PpuMI RGGWCCY 3 cut(s) 464, 1063, 2534
PsiI TTATAA 1 cut(s) 2205
Psp124BI GAGCTC 2 cut(s) 1292, 1880
Psp1406I AACGTT 1 cut(s) 1773
Psp5II RGGWCCY 3 cut(s) 464, 1063, 2534
Psp6I CCWGG 8 cut(s) 54, 247, 730, 1471, 1744, 1861, 2335, 2536
PspCI CACGTG 2 cut(s) 311, 1625
PspFI CCCAGC 6 cut(s) 581, 658, 1672, 1925, 2194, 2513
PspGI CCWGG 8 cut(s) 54, 247, 730, 1471, 1744, 1861, 2335, 2536
PspOMI GGGCCC 1 cut(s) 440
PspPPI RGGWCCY 3 cut(s) 464, 1063, 2534
PstI CTGCAG 3 cut(s) 232, 1128, 1831
PstNI CAGNNNCTG 6 cut(s) 698, 737, 1202, 1709, 1907, 2336
PsuI RGATCY 5 cut(s) 127, 163, 694, 2251, 2312
PsyI GACNNNGTC 1 cut(s) 360
PvuII CAGCTG 4 cut(s) 1081, 1832, 1925, 2198
RsaI GTAC 5 cut(s) 702, 967, 1255, 1656, 1763
RsaNI GTAC 5 cut(s) 701, 966, 1254, 1655, 1762
RseI CAYNNNNRTG 2 cut(s) 449, 1188
Rsr2I CGGWCCG 1 cut(s) 1302
RsrII CGGWCCG 1 cut(s) 1302
SacI GAGCTC 2 cut(s) 1292, 1880
SalI GTCGAC 1 cut(s) 906
SapI GCTCTTC 2 cut(s) 877, 2194
SaqAI TTAA 4 cut(s) 180, 588, 750, 2357
Sau3AI GATC 9 cut(s) 51, 69, 127, 163, 694, 859, 1396, 2251, 2312
ScaI AGTACT 1 cut(s) 1255
SchI GAGTC 5 cut(s) 233, 1101, 1594, 1622, 2106
SduI GDGCHC 7 cut(s) 444, 746, 1273, 1292, 1572, 1880, 2218
SfaNI GCATC 4 cut(s) 304, 487, 671, 1920
SfcI CTRYAG 6 cut(s) 228, 1056, 1124, 1552, 1827, 1966
SinI GGWCC 7 cut(s) 338, 356, 464, 506, 1063, 1302, 2534
SmiMI CAYNNNNRTG 2 cut(s) 449, 1188
SmlI CTYRAG 4 cut(s) 1087, 1348, 1617, 2049
SmoI CTYRAG 4 cut(s) 1087, 1348, 1617, 2049
SspI AATATT 1 cut(s) 1694
SspMI CTAG 3 cut(s) 468, 1136, 1418
SstI GAGCTC 2 cut(s) 1292, 1880
StyI CCWWGG 2 cut(s) 2425, 2508
TaaI ACNGT 7 cut(s) 38, 86, 926, 1302, 1556, 1970, 2227
TaiI ACGT 5 cut(s) 313, 757, 1627, 1776, 2243
TaqI TCGA 3 cut(s) 907, 1880, 1915
TatI WGTACW 4 cut(s) 700, 1253, 1654, 1761
TauI GCSGC 3 cut(s) 12, 1725, 2116
TfiI GAWTC 7 cut(s) 208, 233, 719, 1100, 1199, 1328, 1484
Tru1I TTAA 4 cut(s) 180, 588, 750, 2357
Tru9I TTAA 4 cut(s) 180, 588, 750, 2357
TscAI CASTG 7 cut(s) 91, 567, 651, 675, 1468, 1504, 2115
TseFI GTSAC 5 cut(s) 522, 933, 1074, 1849, 1970
Tsp45I GTSAC 5 cut(s) 522, 933, 1074, 1849, 1970
TspDTI ATGAA 6 cut(s) 864, 1395, 1497, 2355, 2471, 2591
TspRI CASTG 7 cut(s) 91, 567, 651, 675, 1468, 1504, 2115
Tth111I GACNNNGTC 1 cut(s) 360
Van91I CCANNNNNTGG 3 cut(s) 1925, 2020, 2509
VpaK11BI GGWCC 7 cut(s) 338, 356, 464, 506, 1063, 1302, 2534
XagI CCTNNNNNAGG 1 cut(s) 807
XapI RAATTY 2 cut(s) 286, 590
XceI RCATGY 1 cut(s) 2570
XcmI CCANNNNNNNNNTGG 2 cut(s) 494, 2510
XmiI GTMKAC 1 cut(s) 907
XspI CTAG 3 cut(s) 468, 1136, 1418
ZrmI AGTACT 1 cut(s) 1255
Zsp2I ATGCAT 1 cut(s) 2287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.