MD09G1158600.v1.1

Xyloglucan glycosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003794
Physical Location & Seq
Forward (+)
12677713 .. 12681028
3316 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1158600.v1.1.491

Sequence Viewer

Length: 2061 bp
ATGGCTCCAAGCCTAAATTTTTCAAGGTGGTGGGGAAAGGAGAGGACAAGTAAGGGAAATCCAGTGGTGGTAACAATGGAGAACCCTAATTACTCAGTGCTGGAGATAAACGGTCCAGATGAAGTTTTCAGGCCAGTTGATAAGGACAGAGGGAAGAATGCTAGGCAATTTACATGGGCTTTGCTTCTAAAAGCCCACAAAGCTGTTGGCTTTGTTTCTTGGATTGGAAACATTGTTTGGTCATTGCTTGGTTCAATTAAAAAAAGATTGATTTTTGGGACCGTGGAGACTGAGAAATCTGGAAAAGGGAGGATTTTGTATAGGGTTATTATGGTGTTCTTAATGATGGCTTTGGCTTTTCTGGCTTTTGAATTGATGGCTCACTTCAAGGGTTGGCATTTGCATATCCCACAGAGTTTGGAGATTAGAGGATGGCTTCACTCGATTTACTTTTCGTGGTTGGAGTTCCGGGCCGGCTACATTGCTCCTGCAATTCAGGGTTTGACTAACTTCTGTGTGGCTCTCTTCTTAATCCAGTCCGCCGACCGGATGCTGCTGTGTTTAGGTTGCTTCTGGATCAAGTTTAAGAAGATTAAGCCAAAGTTTGAAGTTCCATTGAAGTCAGACGATTTGGAAGCTCCAGGATGCAATTATCCGAAAGTTCTTGTTCAAATTCCTATGTGTAATGAGAGAGAGGTATATGAACAATCTATTTCAGCAGTCTGCCAACTCGATTGGCCGAAAGAACGCTTGCTGATTCAAGTTCTGGACGACTCTGATGATGAGAGCATACAATGGTTAATCAAGGGGGAGGTAGCCAAGTGGAACCAAAGGGGTGTCAATATCATCTATCGCCATCGTTTGGTCAGAACTGGTTACAAAGCTGGAAATCTCAAGTCTGCAATGAATTGTGATTATGTCAAGGACTATGAGTTTGTTGCAATCTTTGATGCCGATTTCCAGCCAAACCCTGACTACCTCAAGCTCACAGTTCCCCATTTTAAGGACAATCCTGAGCTCGGGTTAGTTCAGGCTAGGTGGTCTTTTGTCAACACAGATGAGAACTTGTTGACACGCCTCCAGAACATTAATTTGTGCTTCCACTTTGAGGTTGAACAGCAGGTTAATGGGGTTTTCCTCAATTTCTTTGGTTTCAATGGTACTGCTGGAGTTTGGAGAATCAAAGCACTTGAAGAATCTGGAGGCTGGCTTGAGCGTACAACAGTAGAAGATATGGATATAGCAGTTCGTGCCCATCTTTGTGGTTGGAAATTCATATTCCTCAATGATGTCAAGGTACTTTGTGAGCTTCCAGAATCTTATGAAGCTTACAAAAAGCAACAACATCGTTGGCATTCTGGTCCCATGCATCTTTTCCGTTTGTGCCTTCCAGCAATTATTAGTTCTAAGATGAAGTTCTGGAAGAAAGCAAACTTGATACTACTATTCTTTCTTCTTAGGAAGTTAATTCTCCCATTTTATTCTTTCACATTGTTCTGCATAATTCTTCCTCTGACAATGTTTGTCCCCGAAGCCGAGCTATCCATGTGGGTTATCTGCTATGTGCCTGTATTTATGTCATTCATGAACATTCTTCCTTCCCTTAGATCTTTCCCCTTCATTGTCCCTTACCTCCTGTTCGAAAACACAATGTCCGTGACCAAATTCAACGCTATGGTTTCCGGTTTGTTCAAGTTGGGGAGCTCGTACGAATGGGTTGTCACCAAGAAGGCAGGTCGGTCATCGGAGCCGGACCTGCTAGAATTAGAGGAGAGGGAAACAAAGGCCATGATTCATCCACAACTCTACCGAGGAACATCAGATAGTGGCCTCTCGGAGCTCAACAAATTAAAAGAGCATCAAGAAGCTGCTCCGAAACCTCCTCCTGTGAAGAAACTGAACAAGATATACAAGAAAGAGCTAGCTCTGGCTTTCTTACTGCTTACTGCTGCACTTAGGAGCCTCCTATCAGCACAAGGGGTACACTTTTACTTCCTTCTATTCCAAGGTGTGTCATTTTTACTGGTGGGTCTTGACCTAATTGGTGAGCAGATGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_tranf_2_3 Glyco_trans_2_3

Protein Analysis

687

Amino Acids

79.11

Weight (kDa)

8.91

Isoelectric Point (pI)

35.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_tranf_2_3 PF13641 219 - 452 5.1e-11 Glycosyltransferase like family 2
Glycos_transf_2 PF00535 224 - 372 4.6e-08 Glycosyl transferase family 2
Glyco_transf_21 PF13506 294 - 452 2.6e-07 Glycosyl transferase family 21
Glyco_trans_2_3 PF13632 313 - 526 1.1e-18 Glycosyl transferase family group 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 1111, 1724, 1764
AccB7I CCANNNNNTGG 1 cut(s) 862
AciI CCGC 1 cut(s) 540
AclWI GGATC 1 cut(s) 584
AcoI YGGCCR 1 cut(s) 737
AcsI RAATTY 4 cut(s) 16, 672, 1271, 1664
AfaI GTAC 5 cut(s) 1162, 1219, 1299, 1709, 1983
AfiI CCNNNNNNNGG 5 cut(s) 546, 862, 1003, 1019, 1108
AjnI CCWGG 1 cut(s) 640
AjuI GAANNNNNNNTTGG 2 cut(s) 220, 252
Alw21I GWGCWC 3 cut(s) 1020, 1706, 1842
Alw26I GTCTC 1 cut(s) 281
AlwI GGATC 1 cut(s) 584
Ama87I CYCGRG 1 cut(s) 1019
AoxI GGCC 5 cut(s) 131, 471, 737, 1785, 1828
ApeKI GCWGC 3 cut(s) 553, 1868, 1949
ApoI RAATTY 4 cut(s) 16, 672, 1271, 1664
ArsI GACNNNNNNTTYG 4 cut(s) 917, 949, 1704, 1736
AseI ATTAAT 1 cut(s) 1089
AspS9I GGNCC 5 cut(s) 113, 279, 471, 1361, 1753
AsuC2I CCSGG 1 cut(s) 470
AsuHPI GGTGA 2 cut(s) 1714, 2057
AsuII TTCGAA 1 cut(s) 1641
AsuNHI GCTAGC 1 cut(s) 1921
AvaI CYCGRG 1 cut(s) 1019
AvaII GGWCC 4 cut(s) 113, 279, 1361, 1753
BaeGI GKGCMC 1 cut(s) 1255
BanII GRGCYC 3 cut(s) 1020, 1706, 1842
Bbv12I GWGCWC 3 cut(s) 1020, 1706, 1842
BbvI GCAGC 3 cut(s) 540, 1855, 1936
BccI CCATC 5 cut(s) 340, 370, 426, 864, 1263
BcgI CGANNNNNNTGC 2 cut(s) 1328, 1362
BciT130I CCWGG 1 cut(s) 642
BcnI CCSGG 1 cut(s) 470
BcoDI GTCTC 1 cut(s) 281
BfaI CTAG 5 cut(s) 162, 1035, 1760, 1922, 2059
BfuAI ACCTGC 3 cut(s) 1111, 1724, 1764
BglII AGATCT 1 cut(s) 1607
BisI GCNGC 3 cut(s) 554, 1869, 1950
BlsI GCNGC 3 cut(s) 555, 1870, 1951
Bme1390I CCNGG 2 cut(s) 470, 642
Bme18I GGWCC 4 cut(s) 113, 279, 1361, 1753
BmeT110I CYCGRG 1 cut(s) 1019
BmgT120I GGNCC 5 cut(s) 113, 279, 471, 1361, 1753
BmiI GGNNCC 6 cut(s) 6, 280, 827, 1363, 1749, 1961
BmrFI CCNGG 2 cut(s) 470, 642
BmsI GCATC 5 cut(s) 540, 635, 940, 1378, 1867
BmtI GCTAGC 1 cut(s) 1925
BpmI CTGGAG 5 cut(s) 122, 624, 1064, 1188, 1221
Bpu10I CCTNAGC 1 cut(s) 1014
Bpu14I TTCGAA 1 cut(s) 1641
BpuEI CTTGAG 3 cut(s) 878, 965, 1232
BpuMI CCSGG 1 cut(s) 470
BsaJI CCNNGG 3 cut(s) 282, 1810, 2005
BsaWI WCCGGW 2 cut(s) 546, 1682
Bsc4I CCNNNNNNNGG 5 cut(s) 546, 862, 1003, 1019, 1108
Bse118I RCCGGY 1 cut(s) 473
Bse1I ACTGG 5 cut(s) 62, 134, 535, 877, 2028
Bse3DI GCAATG 3 cut(s) 242, 480, 909
BseBI CCWGG 1 cut(s) 642
BseDI CCNNGG 3 cut(s) 282, 1810, 2005
BseGI GGATG 4 cut(s) 437, 555, 650, 1795
BseLI CCNNNNNNNGG 5 cut(s) 546, 862, 1003, 1019, 1108
BseMI GCAATG 3 cut(s) 242, 480, 909
BseMII CTCAG 3 cut(s) 108, 282, 1005
BseNI ACTGG 5 cut(s) 62, 134, 535, 877, 2028
BseRI GAGGAG 2 cut(s) 1784, 1872
BseSI GKGCMC 1 cut(s) 1255
BseXI GCAGC 3 cut(s) 540, 1855, 1936
BsgI GTGCAG 1 cut(s) 1935
Bsh1285I CGRYCG 1 cut(s) 547
BshFI GGCC 5 cut(s) 133, 473, 739, 1787, 1830
BsiEI CGRYCG 1 cut(s) 547
BsiHKAI GWGCWC 3 cut(s) 1020, 1706, 1842
BsiHKCI CYCGRG 1 cut(s) 1019
BsiSI CCGG 5 cut(s) 469, 474, 547, 1683, 1751
BsiWI CGTACG 1 cut(s) 1707
BslFI GGGAC 4 cut(s) 292, 1347, 1511, 1610
BslI CCNNNNNNNGG 5 cut(s) 546, 862, 1003, 1019, 1108
BsmAI GTCTC 1 cut(s) 281
BsmFI GGGAC 4 cut(s) 292, 1347, 1511, 1610
BsmI GAATGC 2 cut(s) 163, 1354
BsnI GGCC 5 cut(s) 133, 473, 739, 1787, 1830
BsoBI CYCGRG 1 cut(s) 1019
Bsp119I TTCGAA 1 cut(s) 1641
Bsp1286I GDGCHC 4 cut(s) 1020, 1255, 1706, 1842
Bsp143I GATC 2 cut(s) 576, 1607
BspACI CCGC 1 cut(s) 540
BspANI GGCC 5 cut(s) 133, 473, 739, 1787, 1830
BspCNI CTCAG 3 cut(s) 107, 283, 1006
BspHI TCATGA 1 cut(s) 1584
BspLI GGNNCC 6 cut(s) 6, 280, 827, 1363, 1749, 1961
BspMI ACCTGC 3 cut(s) 1111, 1724, 1764
BspOI GCTAGC 1 cut(s) 1925
BspPI GGATC 1 cut(s) 584
BspT104I TTCGAA 1 cut(s) 1641
BsrDI GCAATG 3 cut(s) 242, 480, 909
BsrFI RCCGGY 1 cut(s) 473
BsrI ACTGG 5 cut(s) 62, 134, 535, 877, 2028
BssAI RCCGGY 1 cut(s) 473
BssECI CCNNGG 3 cut(s) 282, 1810, 2005
BssMI GATC 2 cut(s) 576, 1607
BssT1I CCWWGG 1 cut(s) 2005
Bst2UI CCWGG 1 cut(s) 642
Bst4CI ACNGT 4 cut(s) 113, 283, 991, 1225
Bst6I CTCTTC 1 cut(s) 530
BstAPI GCANNNNNTGC 1 cut(s) 1250
BstBI TTCGAA 1 cut(s) 1641
BstC8I GCNNGC 4 cut(s) 475, 752, 1208, 1923
BstDEI CTNAG 7 cut(s) 94, 291, 1014, 1407, 1457, 1604, 1955
BstDSI CCRYGG 1 cut(s) 282
BstF5I GGATG 4 cut(s) 437, 555, 650, 1795
BstKTI GATC 2 cut(s) 579, 1610
BstMAI GTCTC 1 cut(s) 281
BstMBI GATC 2 cut(s) 576, 1607
BstMCI CGRYCG 1 cut(s) 547
BstMWI GCNNNNNNNGC 4 cut(s) 200, 362, 1250, 1756
BstNI CCWGG 1 cut(s) 642
BstSCI CCNGG 2 cut(s) 468, 640
BstSLI GKGCMC 1 cut(s) 1255
BstV1I GCAGC 3 cut(s) 540, 1855, 1936
BstX2I RGATCY 1 cut(s) 1607
BstXI CCANNNNNNTGG 1 cut(s) 1262
BstYI RGATCY 1 cut(s) 1607
BsuRI GGCC 5 cut(s) 133, 473, 739, 1787, 1830
BtgI CCRYGG 1 cut(s) 282
BtsCI GGATG 4 cut(s) 437, 555, 650, 1795
BtsIMutI CAGTG 2 cut(s) 69, 102
BveI ACCTGC 3 cut(s) 1111, 1724, 1764
Cac8I GCNNGC 4 cut(s) 475, 752, 1208, 1923
CciI TCATGA 1 cut(s) 1584
Cfr10I RCCGGY 1 cut(s) 473
Cfr13I GGNCC 5 cut(s) 113, 279, 471, 1361, 1753
Csp6I GTAC 5 cut(s) 1161, 1218, 1298, 1708, 1982
CspCI CAANNNNNGTGG 2 cut(s) 399, 434
CviAII CATG 5 cut(s) 174, 1366, 1546, 1585, 1789
CviQI GTAC 5 cut(s) 1161, 1218, 1298, 1708, 1982
DdeI CTNAG 7 cut(s) 94, 291, 1014, 1407, 1457, 1604, 1955
DpnI GATC 2 cut(s) 578, 1609
DpnII GATC 2 cut(s) 576, 1607
EaeI YGGCCR 1 cut(s) 737
Eam1104I CTCTTC 1 cut(s) 530
EarI CTCTTC 1 cut(s) 530
EciI GGCGGA 1 cut(s) 529
Ecl136II GAGCTC 3 cut(s) 1018, 1704, 1840
Eco130I CCWWGG 1 cut(s) 2005
Eco24I GRGCYC 3 cut(s) 1020, 1706, 1842
Eco47I GGWCC 4 cut(s) 113, 279, 1361, 1753
Eco53kI GAGCTC 3 cut(s) 1018, 1704, 1840
Eco88I CYCGRG 1 cut(s) 1019
EcoICRI GAGCTC 3 cut(s) 1018, 1704, 1840
EcoRII CCWGG 1 cut(s) 640
EcoT14I CCWWGG 1 cut(s) 2005
EcoT22I ATGCAT 1 cut(s) 1371
EcoT38I GRGCYC 3 cut(s) 1020, 1706, 1842
ErhI CCWWGG 1 cut(s) 2005
FaeI CATG 5 cut(s) 177, 1369, 1549, 1588, 1792
FaqI GGGAC 4 cut(s) 292, 1347, 1511, 1610
FatI CATG 5 cut(s) 173, 1365, 1545, 1584, 1788
Fnu4HI GCNGC 3 cut(s) 554, 1869, 1950
FokI GGATG 4 cut(s) 444, 562, 657, 1782
FriOI GRGCYC 3 cut(s) 1020, 1706, 1842
Fsp4HI GCNGC 3 cut(s) 554, 1869, 1950
FspBI CTAG 5 cut(s) 162, 1035, 1760, 1922, 2059
GluI GCNGC 3 cut(s) 554, 1869, 1950
GsuI CTGGAG 5 cut(s) 122, 624, 1064, 1188, 1221
HaeIII GGCC 5 cut(s) 133, 473, 739, 1787, 1830
HapII CCGG 5 cut(s) 469, 474, 547, 1683, 1751
Hin1II CATG 5 cut(s) 177, 1369, 1549, 1588, 1792
HincII GTYRAC 2 cut(s) 1051, 1071
HindII GTYRAC 2 cut(s) 1051, 1071
HindIII AAGCTT 1 cut(s) 1326
HinfI GANTC 6 cut(s) 757, 773, 1179, 1196, 1316, 1792
HpaII CCGG 5 cut(s) 469, 474, 547, 1683, 1751
HphI GGTGA 2 cut(s) 1714, 2057
Hpy166II GTNNAC 3 cut(s) 1051, 1071, 1984
Hpy188I TCNGA 9 cut(s) 625, 657, 778, 869, 1515, 1747, 1822, 1837, 1875
Hpy8I GTNNAC 3 cut(s) 1051, 1071, 1984
HpyAV CCTTC 5 cut(s) 1397, 1608, 1627, 1723, 2006
HpyCH4III ACNGT 4 cut(s) 113, 283, 991, 1225
HpyCH4V TGCA 8 cut(s) 403, 491, 648, 902, 941, 1369, 1500, 1952
HpyF10VI GCNNNNNNNGC 4 cut(s) 200, 362, 1250, 1756
HpyF3I CTNAG 7 cut(s) 94, 291, 1014, 1407, 1457, 1604, 1955
Hsp92II CATG 5 cut(s) 177, 1369, 1549, 1588, 1792
KroI GCCGGC 1 cut(s) 473
KroNI GCCGGC 1 cut(s) 475
Kzo9I GATC 2 cut(s) 576, 1607
LmnI GCTCC 8 cut(s) 10, 490, 643, 1701, 1747, 1837, 1876, 1959
Lsp1109I GCAGC 3 cut(s) 540, 1855, 1936
LweI GCATC 5 cut(s) 540, 635, 940, 1378, 1867
MaeI CTAG 5 cut(s) 162, 1035, 1760, 1922, 2059
MaeIII GTNAC 4 cut(s) 70, 875, 1657, 1720
MalI GATC 2 cut(s) 578, 1609
MboI GATC 2 cut(s) 576, 1607
MflI RGATCY 1 cut(s) 1607
MhlI GDGCHC 4 cut(s) 1020, 1255, 1706, 1842
MlyI GAGTC 1 cut(s) 767
MmeI TCCRAC 2 cut(s) 441, 1247
Mph1103I ATGCAT 1 cut(s) 1371
MroNI GCCGGC 1 cut(s) 473
MslI CAYNNNNRTG 1 cut(s) 1260
MspI CCGG 5 cut(s) 469, 474, 547, 1683, 1751
MspR9I CCNGG 2 cut(s) 470, 642
Mva1269I GAATGC 2 cut(s) 163, 1354
MvaI CCWGG 1 cut(s) 642
MwoI GCNNNNNNNGC 4 cut(s) 200, 362, 1250, 1756
NaeI GCCGGC 1 cut(s) 475
NciI CCSGG 1 cut(s) 470
NdeII GATC 2 cut(s) 576, 1607
NgoMIV GCCGGC 1 cut(s) 473
NheI GCTAGC 1 cut(s) 1921
NlaIII CATG 5 cut(s) 177, 1369, 1549, 1588, 1792
NlaIV GGNNCC 6 cut(s) 6, 280, 827, 1363, 1749, 1961
NmeAIII GCCGAG 1 cut(s) 1561
NmuCI GTSAC 2 cut(s) 1657, 1720
NsiI ATGCAT 1 cut(s) 1371
NspV TTCGAA 1 cut(s) 1641
PagI TCATGA 1 cut(s) 1584
PctI GAATGC 2 cut(s) 163, 1354
PdiI GCCGGC 1 cut(s) 475
PfeI GAWTC 5 cut(s) 757, 1179, 1196, 1316, 1792
Pfl23II CGTACG 1 cut(s) 1707
PflMI CCANNNNNTGG 1 cut(s) 862
PfoI TCCNGGA 1 cut(s) 640
PkrI GCNGC 3 cut(s) 555, 1870, 1951
PleI GAGTC 1 cut(s) 767
PpsI GAGTC 1 cut(s) 767
PshBI ATTAAT 1 cut(s) 1089
Psp124BI GAGCTC 3 cut(s) 1020, 1706, 1842
Psp6I CCWGG 1 cut(s) 640
PspGI CCWGG 1 cut(s) 640
PspLI CGTACG 1 cut(s) 1707
PspN4I GGNNCC 6 cut(s) 6, 280, 827, 1363, 1749, 1961
PspPI GGNCC 5 cut(s) 113, 279, 471, 1361, 1753
PsrI GAACNNNNNNTAC 4 cut(s) 74, 106, 1892, 1924
PsuI RGATCY 1 cut(s) 1607
RsaI GTAC 5 cut(s) 1162, 1219, 1299, 1709, 1983
RsaNI GTAC 5 cut(s) 1161, 1218, 1298, 1708, 1982
RseI CAYNNNNRTG 1 cut(s) 1260
SacI GAGCTC 3 cut(s) 1020, 1706, 1842
SatI GCNGC 3 cut(s) 554, 1869, 1950
Sau3AI GATC 2 cut(s) 576, 1607
Sau96I GGNCC 5 cut(s) 113, 279, 471, 1361, 1753
SchI GAGTC 1 cut(s) 767
ScrFI CCNGG 2 cut(s) 470, 642
SduI GDGCHC 4 cut(s) 1020, 1255, 1706, 1842
SfaNI GCATC 5 cut(s) 540, 635, 940, 1378, 1867
SfuI TTCGAA 1 cut(s) 1641
SinI GGWCC 4 cut(s) 113, 279, 1361, 1753
SmiMI CAYNNNNRTG 1 cut(s) 1260
SmlI CTYRAG 3 cut(s) 893, 980, 1211
SmoI CTYRAG 3 cut(s) 893, 980, 1211
SsiI CCGC 1 cut(s) 540
SspMI CTAG 5 cut(s) 162, 1035, 1760, 1922, 2059
SstI GAGCTC 3 cut(s) 1020, 1706, 1842
StyD4I CCNGG 2 cut(s) 468, 640
StyI CCWWGG 1 cut(s) 2005
TaaI ACNGT 4 cut(s) 113, 283, 991, 1225
TaqI TCGA 3 cut(s) 443, 732, 1641
TaqII GACCGA 1 cut(s) 1728
TfiI GAWTC 5 cut(s) 757, 1179, 1196, 1316, 1792
TscAI CASTG 2 cut(s) 69, 102
TseFI GTSAC 2 cut(s) 1657, 1720
TseI GCWGC 3 cut(s) 553, 1868, 1949
Tsp45I GTSAC 2 cut(s) 1657, 1720
TspGWI ACGGA 2 cut(s) 1367, 1645
TspRI CASTG 2 cut(s) 69, 102
Van91I CCANNNNNTGG 1 cut(s) 862
VpaK11BI GGWCC 4 cut(s) 113, 279, 1361, 1753
VspI ATTAAT 1 cut(s) 1089
XapI RAATTY 4 cut(s) 16, 672, 1271, 1664
XcmI CCANNNNNNNNNTGG 1 cut(s) 203
XspI CTAG 5 cut(s) 162, 1035, 1760, 1922, 2059
Zsp2I ATGCAT 1 cut(s) 1371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.