Rroxscaffold_2G00097420

Xyloglucan glycosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003794
Physical Location & Seq
Forward (+)
18635345 .. 18638789
3445 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00097420.1

Sequence Viewer

Length: 1515 bp
ATGCTGCTTTGTTTAGGTTGCTTCTGGATCAAGTTCAAGAAGATTAAGCCAAGAATTGAAGAAGTTCCATACAAGCCAGACGATTTGGAAGGTTCAGGTCGTAATTACCCCAAGGTTCTTGTTCAAATTCCAATGTGTAATGAGAGAGAGGTATATGAACAATCTATTTCAGCAGTATGCCAACTTGATTGGCCTAAAGAACGCCTTCTGATTCAAGTTCTAGATGATTCTGATGATGAGAGCATACAGTGGTTAATTAAGGCAGAGGTAACTCAGTGGAGCCAAAAAGGTGTCAATATAATCTACCGGCATCGTTTGGTTAGAACTGGTTATAAAGCTGGGAATCTCAAGTCTGCAATGAATTGTGATTATGTGAAGGACTATGAGTTTGTTGCAATCTTTGATGCTGATTTCCAACCAAACCCTGACTTCCTCAAGCTGACAGTTCCCCATTTTAAGGACAATCCTGAGCTTGGGTTGGTTCAGGCGAGGTGGTCTTTTGTTAACAAGGATGAAAACTTGTTGACACGCCTCCAAAATATCAATTTGTGCTTCCACTTTGAAGTGGAACAGCAGGTTAATGGGGTTTTCCTCAATTTCTTTGGCTTCAATGGTACTGCTGGAGTTTGGAGAATTAAAGCACTTGAAGACTCTGGCGGTTGGCTTGAGCGTACAACAGTAGAAGACATGGATATAGCAGTTCGCGCTCATCTTAATGGGTGGAAGTTCATATTCCTTAATGATGTCAAGGTCCTCTGTGAACTTCCCGAGTCTTACGAAGCTTATAAAAAACAGCAACACCGTTGGCATTCTGGTCCGATGCATCTCTTCCGTTTGTGCCTCCCTGCAATAATTACTTCAAAGATGATGTTCTGGAAAAAGGCAAACTTGATACTACTATTCTTTCTCCTTAGGAAGTTAATCCTCCCATTTTACTCTTTCACATTGTTTTGCATAATACTTCCTTTGACCATGTTTGTCCCCGAAGCTGAGCTATCCATGTGGGTTATATGCTACGTGCCCGTTTTTATGTCATTCATGAACATTCTTCCTTCTCCTAGATCTTTTCCCTTCATTGTCCCCTACCTCCTGTTTGAAAACACAATGTCTGTGACCAAATTCAATGCCATGGTATCTGGGTTGTTCCAGTTGGGGAGCTCATATGAATGGATTGTAACCAAGAAGGCGGGACGATCATCAGAATCTGATCTGTTAGCTGTGGAGGAGAGGGAAACGAAGGCCATGAACCACCCACAACTTCATAGAGGAACTTCAGACAGTGGTCTCTCTGAGCTCAACAAGTTAAAAGAGCATCAAGAAGCTGCTGCTCCTAAACCTCCTGTAAAGAAATTGAACAAGATCTACAAGAAAGAGCTGGCACTCGCTTTCTTACTGCTCACTGCTGCAGGTAGAAGCCTTCTATCAGCTCAAGGGGTACATTTTTACTTCCTACTATTTCAAGGCATATCATTTCTACTGGTCGGTCTTGACCTGATTGGTGAGCAGATGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_tranf_2_3 Glyco_trans_2_3

Protein Analysis

504

Amino Acids

58.21

Weight (kDa)

7.94

Isoelectric Point (pI)

39.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_tranf_2_3 PF13641 37 - 270 1.3e-12 Glycosyltransferase like family 2
Glycos_transf_2 PF00535 42 - 191 4e-09 Glycosyl transferase family 2
Glyco_transf_21 PF13506 126 - 270 4.9e-07 Glycosyl transferase family 21
Glyco_trans_2_3 PF13632 131 - 343 1.5e-19 Glycosyl transferase family group 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 333, 786
Acc36I ACCTGC 2 cut(s) 565, 1397
AccII CGCG 1 cut(s) 705
AciI CCGC 2 cut(s) 657, 1187
AclWI GGATC 1 cut(s) 35
AcsI RAATTY 2 cut(s) 126, 1118
AcuI CTGAAG 1 cut(s) 1257
AfaI GTAC 3 cut(s) 616, 673, 1437
AfiI CCNNNNNNNGG 2 cut(s) 457, 473
Alw21I GWGCWC 2 cut(s) 1160, 1296
Alw26I GTCTC 1 cut(s) 1289
AlwI GGATC 1 cut(s) 35
AlwNI CAGNNNCTG 1 cut(s) 1205
Ama87I CYCGRG 1 cut(s) 767
AoxI GGCC 2 cut(s) 191, 1239
ApeKI GCWGC 4 cut(s) 4, 1322, 1325, 1403
ApoI RAATTY 2 cut(s) 126, 1118
ArsI GACNNNNNNTTYG 2 cut(s) 371, 403
Asp700I GAANNNNTTC 2 cut(s) 63, 204
AspLEI GCGC 1 cut(s) 707
AspS9I GGNCC 2 cut(s) 751, 815
AsuHPI GGTGA 1 cut(s) 1511
AvaI CYCGRG 1 cut(s) 767
AvaII GGWCC 2 cut(s) 751, 815
AxyI CCTNAGG 1 cut(s) 911
BaeGI GKGCMC 1 cut(s) 1023
BanII GRGCYC 2 cut(s) 1160, 1296
BbsI GAAGAC 2 cut(s) 654, 690
Bbv12I GWGCWC 2 cut(s) 1160, 1296
BbvI GCAGC 3 cut(s) 1309, 1312, 1390
BcoDI GTCTC 1 cut(s) 1289
BfaI CTAG 3 cut(s) 221, 1059, 1513
BfmI CTRYAG 1 cut(s) 1404
BfuAI ACCTGC 2 cut(s) 565, 1397
BglII AGATCT 2 cut(s) 1061, 1359
BisI GCNGC 4 cut(s) 5, 1323, 1326, 1404
BlpI GCTNAGC 1 cut(s) 990
BlsI GCNGC 4 cut(s) 6, 1324, 1327, 1405
Bme18I GGWCC 2 cut(s) 751, 815
BmeT110I CYCGRG 1 cut(s) 767
BmgT120I GGNCC 2 cut(s) 751, 815
BmiI GGNNCC 1 cut(s) 281
BmsI GCATC 5 cut(s) 319, 394, 810, 832, 1321
BoxI GACNNNNGTC 1 cut(s) 1281
BpiI GAAGAC 2 cut(s) 654, 690
BpmI CTGGAG 1 cut(s) 642
Bpu10I CCTNAGC 1 cut(s) 468
Bpu1102I GCTNAGC 1 cut(s) 990
BpuEI CTTGAG 4 cut(s) 332, 419, 686, 1413
BsaAI YACGTR 1 cut(s) 1018
BsaI GGTCTC 1 cut(s) 1289
BsaJI CCNNGG 2 cut(s) 111, 1128
Bsc4I CCNNNNNNNGG 2 cut(s) 457, 473
Bse118I RCCGGY 1 cut(s) 306
Bse1I ACTGG 3 cut(s) 331, 1147, 1482
Bse21I CCTNAGG 1 cut(s) 911
Bse3DI GCAATG 1 cut(s) 363
BseDI CCNNGG 2 cut(s) 111, 1128
BseGI GGATG 1 cut(s) 517
BseLI CCNNNNNNNGG 2 cut(s) 457, 473
BseMI GCAATG 1 cut(s) 363
BseMII CTCAG 4 cut(s) 287, 459, 981, 1281
BseNI ACTGG 3 cut(s) 331, 1147, 1482
BseRI GAGGAG 1 cut(s) 1238
BseSI GKGCMC 1 cut(s) 1023
BseXI GCAGC 3 cut(s) 1309, 1312, 1390
BseYI CCCAGC 1 cut(s) 338
Bsh1236I CGCG 1 cut(s) 705
BshFI GGCC 2 cut(s) 193, 1241
BsiHKAI GWGCWC 2 cut(s) 1160, 1296
BsiHKCI CYCGRG 1 cut(s) 767
BsiSI CCGG 1 cut(s) 307
BslFI GGGAC 3 cut(s) 965, 1064, 1203
BslI CCNNNNNNNGG 2 cut(s) 457, 473
BsmAI GTCTC 1 cut(s) 1289
BsmFI GGGAC 3 cut(s) 965, 1064, 1203
BsmI GAATGC 1 cut(s) 808
BsnI GGCC 2 cut(s) 193, 1241
Bso31I GGTCTC 1 cut(s) 1289
BsoBI CYCGRG 1 cut(s) 767
Bsp1286I GDGCHC 3 cut(s) 1023, 1160, 1296
Bsp143I GATC 5 cut(s) 27, 1061, 1193, 1207, 1359
Bsp1720I GCTNAGC 1 cut(s) 990
Bsp19I CCATGG 1 cut(s) 1128
BspACI CCGC 2 cut(s) 657, 1187
BspANI GGCC 2 cut(s) 193, 1241
BspCNI CTCAG 4 cut(s) 286, 460, 982, 1282
BspFNI CGCG 1 cut(s) 705
BspHI TCATGA 1 cut(s) 1038
BspLI GGNNCC 1 cut(s) 281
BspMAI CTGCAG 1 cut(s) 1408
BspMI ACCTGC 2 cut(s) 565, 1397
BspPI GGATC 1 cut(s) 35
BspTNI GGTCTC 1 cut(s) 1289
BsrDI GCAATG 1 cut(s) 363
BsrFI RCCGGY 1 cut(s) 306
BsrI ACTGG 3 cut(s) 331, 1147, 1482
BssAI RCCGGY 1 cut(s) 306
BssECI CCNNGG 2 cut(s) 111, 1128
BssMI GATC 5 cut(s) 27, 1061, 1193, 1207, 1359
BssT1I CCWWGG 2 cut(s) 111, 1128
Bst4CI ACNGT 5 cut(s) 249, 445, 679, 803, 1280
Bst6I CTCTTC 1 cut(s) 833
BstBAI YACGTR 1 cut(s) 1018
BstC8I GCNNGC 1 cut(s) 1377
BstDEI CTNAG 5 cut(s) 273, 468, 911, 990, 1290
BstDSI CCRYGG 1 cut(s) 1128
BstF5I GGATG 1 cut(s) 517
BstFNI CGCG 1 cut(s) 705
BstHHI GCGC 1 cut(s) 707
BstKTI GATC 5 cut(s) 30, 1064, 1196, 1210, 1362
BstMAI GTCTC 1 cut(s) 1289
BstMBI GATC 5 cut(s) 27, 1061, 1193, 1207, 1359
BstMWI GCNNNNNNNGC 1 cut(s) 704
BstPAI GACNNNNGTC 1 cut(s) 1281
BstSFI CTRYAG 1 cut(s) 1404
BstSLI GKGCMC 1 cut(s) 1023
BstUI CGCG 1 cut(s) 705
BstV1I GCAGC 3 cut(s) 1309, 1312, 1390
BstV2I GAAGAC 2 cut(s) 654, 690
BstX2I RGATCY 2 cut(s) 1061, 1359
BstYI RGATCY 2 cut(s) 1061, 1359
Bsu36I CCTNAGG 1 cut(s) 911
BsuRI GGCC 2 cut(s) 193, 1241
BtgI CCRYGG 1 cut(s) 1128
BtsCI GGATG 1 cut(s) 517
BtsI GCAGTG 1 cut(s) 1398
BtsIMutI CAGTG 4 cut(s) 254, 281, 1285, 1398
BveI ACCTGC 2 cut(s) 565, 1397
Cac8I GCNNGC 1 cut(s) 1377
CaiI CAGNNNCTG 1 cut(s) 1205
CciI TCATGA 1 cut(s) 1038
CfoI GCGC 1 cut(s) 707
Cfr10I RCCGGY 1 cut(s) 306
Cfr13I GGNCC 2 cut(s) 751, 815
Csp6I GTAC 3 cut(s) 615, 672, 1436
CviAII CATG 6 cut(s) 688, 973, 1000, 1039, 1129, 1243
CviQI GTAC 3 cut(s) 615, 672, 1436
DdeI CTNAG 5 cut(s) 273, 468, 911, 990, 1290
DpnI GATC 5 cut(s) 29, 1063, 1195, 1209, 1361
DpnII GATC 5 cut(s) 27, 1061, 1193, 1207, 1359
Eam1104I CTCTTC 1 cut(s) 833
EarI CTCTTC 1 cut(s) 833
Ecl136II GAGCTC 2 cut(s) 1158, 1294
Eco130I CCWWGG 2 cut(s) 111, 1128
Eco24I GRGCYC 2 cut(s) 1160, 1296
Eco31I GGTCTC 1 cut(s) 1289
Eco47I GGWCC 2 cut(s) 751, 815
Eco53kI GAGCTC 2 cut(s) 1158, 1294
Eco57I CTGAAG 1 cut(s) 1257
Eco81I CCTNAGG 1 cut(s) 911
Eco88I CYCGRG 1 cut(s) 767
EcoICRI GAGCTC 2 cut(s) 1158, 1294
EcoO109I RGGNCCY 1 cut(s) 751
EcoT14I CCWWGG 2 cut(s) 111, 1128
EcoT22I ATGCAT 1 cut(s) 825
EcoT38I GRGCYC 2 cut(s) 1160, 1296
ErhI CCWWGG 2 cut(s) 111, 1128
FaeI CATG 6 cut(s) 691, 976, 1003, 1042, 1132, 1246
FalI AAGNNNNNCTT 4 cut(s) 189, 221, 872, 904
FaqI GGGAC 3 cut(s) 965, 1064, 1203
FatI CATG 6 cut(s) 687, 972, 999, 1038, 1128, 1242
FauI CCCGC 1 cut(s) 1180
FauNDI CATATG 1 cut(s) 1162
Fnu4HI GCNGC 4 cut(s) 5, 1323, 1326, 1404
FokI GGATG 1 cut(s) 524
FriOI GRGCYC 2 cut(s) 1160, 1296
Fsp4HI GCNGC 4 cut(s) 5, 1323, 1326, 1404
FspBI CTAG 3 cut(s) 221, 1059, 1513
GlaI GCGC 1 cut(s) 706
GluI GCNGC 4 cut(s) 5, 1323, 1326, 1404
GsaI CCCAGC 1 cut(s) 342
GsuI CTGGAG 1 cut(s) 642
HaeIII GGCC 2 cut(s) 193, 1241
HapII CCGG 1 cut(s) 307
HhaI GCGC 1 cut(s) 707
Hin1II CATG 6 cut(s) 691, 976, 1003, 1042, 1132, 1246
Hin6I GCGC 1 cut(s) 705
HinP1I GCGC 1 cut(s) 705
HincII GTYRAC 2 cut(s) 505, 525
HindII GTYRAC 2 cut(s) 505, 525
HindIII AAGCTT 1 cut(s) 780
HinfI GANTC 6 cut(s) 211, 227, 343, 650, 770, 1202
HpaI GTTAAC 1 cut(s) 505
HpaII CCGG 1 cut(s) 307
HphI GGTGA 1 cut(s) 1511
Hpy166II GTNNAC 3 cut(s) 505, 525, 761
Hpy188I TCNGA 7 cut(s) 210, 232, 819, 1201, 1207, 1276, 1291
Hpy188III TCNNGA 9 cut(s) 25, 37, 221, 467, 767, 874, 1039, 1316, 1487
Hpy8I GTNNAC 3 cut(s) 505, 525, 761
HpyAV CCTTC 8 cut(s) 83, 215, 370, 1062, 1081, 1177, 1231, 1427
HpyCH4III ACNGT 5 cut(s) 249, 445, 679, 803, 1280
HpyCH4IV ACGT 1 cut(s) 1017
HpyCH4V TGCA 6 cut(s) 356, 395, 823, 848, 954, 1406
HpyF10VI GCNNNNNNNGC 1 cut(s) 704
HpyF3I CTNAG 5 cut(s) 273, 468, 911, 990, 1290
HpySE526I ACGT 1 cut(s) 1017
Hsp92II CATG 6 cut(s) 691, 976, 1003, 1042, 1132, 1246
HspAI GCGC 1 cut(s) 705
KspAI GTTAAC 1 cut(s) 505
Kzo9I GATC 5 cut(s) 27, 1061, 1193, 1207, 1359
LmnI GCTCC 3 cut(s) 279, 1155, 1333
Lsp1109I GCAGC 3 cut(s) 1309, 1312, 1390
LweI GCATC 5 cut(s) 319, 394, 810, 832, 1321
MaeI CTAG 3 cut(s) 221, 1059, 1513
MaeII ACGT 1 cut(s) 1017
MaeIII GTNAC 3 cut(s) 268, 1111, 1174
MalI GATC 5 cut(s) 29, 1063, 1195, 1209, 1361
MboI GATC 5 cut(s) 27, 1061, 1193, 1207, 1359
MboII GAAGA 6 cut(s) 52, 71, 659, 695, 820, 1040
MflI RGATCY 2 cut(s) 1061, 1359
MhlI GDGCHC 3 cut(s) 1023, 1160, 1296
MlyI GAGTC 2 cut(s) 644, 779
MmeI TCCRAC 1 cut(s) 439
Mph1103I ATGCAT 1 cut(s) 825
MroXI GAANNNNTTC 2 cut(s) 63, 204
MslI CAYNNNNRTG 2 cut(s) 714, 1165
MspI CCGG 1 cut(s) 307
Mva1269I GAATGC 1 cut(s) 808
MvnI CGCG 1 cut(s) 705
MwoI GCNNNNNNNGC 1 cut(s) 704
NcoI CCATGG 1 cut(s) 1128
NdeI CATATG 1 cut(s) 1162
NdeII GATC 5 cut(s) 27, 1061, 1193, 1207, 1359
NlaIII CATG 6 cut(s) 691, 976, 1003, 1042, 1132, 1246
NlaIV GGNNCC 1 cut(s) 281
NmuCI GTSAC 1 cut(s) 1111
NsiI ATGCAT 1 cut(s) 825
PacI TTAATTAA 1 cut(s) 258
PagI TCATGA 1 cut(s) 1038
PctI GAATGC 1 cut(s) 808
PdmI GAANNNNTTC 2 cut(s) 63, 204
PfeI GAWTC 4 cut(s) 211, 227, 343, 1202
PkrI GCNGC 4 cut(s) 6, 1324, 1327, 1405
PleI GAGTC 2 cut(s) 644, 778
PpsI GAGTC 2 cut(s) 644, 778
Ppu21I YACGTR 1 cut(s) 1018
PpuMI RGGWCCY 1 cut(s) 751
PshAI GACNNNNGTC 1 cut(s) 1281
PsiI TTATAA 2 cut(s) 333, 786
Psp124BI GAGCTC 2 cut(s) 1160, 1296
Psp5II RGGWCCY 1 cut(s) 751
PspFI CCCAGC 1 cut(s) 338
PspN4I GGNNCC 1 cut(s) 281
PspPI GGNCC 2 cut(s) 751, 815
PspPPI RGGWCCY 1 cut(s) 751
PsrI GAACNNNNNNTAC 2 cut(s) 1346, 1378
PstI CTGCAG 1 cut(s) 1408
PstNI CAGNNNCTG 1 cut(s) 1205
PsuI RGATCY 2 cut(s) 1061, 1359
RsaI GTAC 3 cut(s) 616, 673, 1437
RsaNI GTAC 3 cut(s) 615, 672, 1436
RseI CAYNNNNRTG 2 cut(s) 714, 1165
SacI GAGCTC 2 cut(s) 1160, 1296
SatI GCNGC 4 cut(s) 5, 1323, 1326, 1404
Sau3AI GATC 5 cut(s) 27, 1061, 1193, 1207, 1359
Sau96I GGNCC 2 cut(s) 751, 815
SchI GAGTC 2 cut(s) 644, 779
SduI GDGCHC 3 cut(s) 1023, 1160, 1296
SfaNI GCATC 5 cut(s) 319, 394, 810, 832, 1321
SfcI CTRYAG 1 cut(s) 1404
SinI GGWCC 2 cut(s) 751, 815
SmiMI CAYNNNNRTG 2 cut(s) 714, 1165
SmlI CTYRAG 4 cut(s) 347, 434, 665, 1428
SmoI CTYRAG 4 cut(s) 347, 434, 665, 1428
SsiI CCGC 2 cut(s) 657, 1187
SspMI CTAG 3 cut(s) 221, 1059, 1513
SstI GAGCTC 2 cut(s) 1160, 1296
StyI CCWWGG 2 cut(s) 111, 1128
TaaI ACNGT 5 cut(s) 249, 445, 679, 803, 1280
TaiI ACGT 1 cut(s) 1020
TaqII GACCGA 1 cut(s) 1472
TfiI GAWTC 4 cut(s) 211, 227, 343, 1202
TscAI CASTG 4 cut(s) 254, 281, 1285, 1405
TseFI GTSAC 1 cut(s) 1111
TseI GCWGC 4 cut(s) 4, 1322, 1325, 1403
Tsp45I GTSAC 1 cut(s) 1111
TspGWI ACGGA 1 cut(s) 821
TspRI CASTG 4 cut(s) 254, 281, 1285, 1405
VpaK11BI GGWCC 2 cut(s) 751, 815
XapI RAATTY 2 cut(s) 126, 1118
XbaI TCTAGA 1 cut(s) 220
XmnI GAANNNNTTC 2 cut(s) 63, 204
XspI CTAG 3 cut(s) 221, 1059, 1513
Zsp2I ATGCAT 1 cut(s) 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.