MD09G1185000.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0022348
Physical Location & Seq
Reverse (-)
16025415 .. 16027491
2077 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1185000.v1.1.491

Sequence Viewer

Length: 1356 bp
ATGCACCGGGTTGGCGTATCACCATCTGGGTTCACCTTCTCTTCCGTTCTCAATGCCTGTGGTCGTGTCCAGGCGGTTTTCGAAGGGAAACAAGTCCACGCGAGGCTTCTGCAGTGTGGTTTCTTGGGAAACGGGATAGTGCAAACTGCGCTTCTTCATATGTATGCAAAATGTGGGCTTGTAAGGGACGCGAGGGATGTTTTTGATTCGATGGAAGATAAGGATTTGGTTGCTTGGACGGCCATGATTTGTGGGTATTCGAAGATGGGATTGATGTGTGAAGCGCGGTGGTTGTTTGATAACATGGGGCAGTGCAATGCGGTTTCTTGGGCTACCATGGTTGCTGGGTACGCGAACATTGGTGATATGGAAAGGGCAAAGGAATTGTACGAAAGAATGGTGGAGAAAAATCCAGTGGTGTGGGTGGCTATGATAGCGGGATATGGGAAGCGTGGTAATGTAGTGGAGGCTGAGATGGTTTTTGACGAGATACAGATGCCGGATGCATCATGTTGGGCAGCAATGGTGGCTTGTTATGCTCAGAATGGTTATGCAGCAGAAGCCATTGAGATGTATAAGAAAATGAGGGAAGCAAAAGTAAGAGTCAGTGAGGTGGCAATGGTGGGAGCTATTTCAGCTTGTACACAACTTGGGGATGTTAAAATTGCAGCCGCATTGGCAAAGCATGTGGAGGAAGGGTTTTGTGAGAAGACAATCTTTGTATCCAATGCATTGATCCATATGCATTCCAAATGCGGAAACATAGACCAGGCATGGAGAGAGTTCAACAGAATGAGCATGAGAGATGTCATATCCTATAGTGCGTTGGTCACTGCCCTTGCTGACCATGGAAAGGCCGAGGATGCATTGGATCTTTTCTCGAAGATGCAAAAGGAAGGAGTTAGACCGAACGAGGTTACATTCGTCGGTCTCCTCAACGCATGTAGCCATGCCGGACTGGTTGAGAAAGGGTGCCGGTATTTTGAGCTCATGACTCAGGTTTTCGGCATTGAGCCACAAAAGGAGCACTATGCTTGCATGGTTGATCTTCTTGGAAGGGCTGGGATGCTTGAAAAGGCATACAATCTTATAATAGACAATGTCGGTGTTGCCGATGCAAAGATTTGGGGCTCCTTGTTAGGAGCTTGCAAGGTTCATGGCAATGCTGAGTTGAGCGAGATGGCAGCCAGGCAACTCTTCGAAATAGAACCGCGCGATGCAGGAAATTACGTGCTTTTGGCAAATACTTACGCAGAAACAAATAAATGGGATGATGCTGAGAGAGTGAGAATGATGATGAATCAAAGAGGCATGGTAAAATCTCCTGGGTGTAGCTGGAGAGATTCTACTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

452

Amino Acids

49.9

Weight (kDa)

5.88

Isoelectric Point (pI)

37.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 212 - 364 8.5e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 268 - 316 6.3e-13 PPR repeat family
PPR_3 PF13812 269 - 315 1.2e-08 Pentatricopeptide repeat domain
PPR PF01535 271 - 301 3.4e-06 PPR repeat
E_motif PF20431 386 - 446 4.3e-21 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022348)

Species Orthologous Gene IDs
malus_domestica MD09G1185000.v1.1 MD09G1185300.v1.1
prunus_persica Prupe.3G043100_v2.0.a1
pyrus_communis pycom09g10260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1091
AccB1I GGYRCC 1 cut(s) 972
AccII CGCG 6 cut(s) 101, 191, 286, 353, 1213, 1215
AciI CCGC 7 cut(s) 74, 286, 320, 437, 672, 756, 1211
AclWI GGATC 2 cut(s) 730, 879
AcoI YGGCCR 1 cut(s) 240
AfaI GTAC 3 cut(s) 350, 389, 643
AfiI CCNNNNNNNGG 1 cut(s) 853
AgsI TTSAA 2 cut(s) 787, 1073
AjnI CCWGG 4 cut(s) 69, 768, 1187, 1324
AluBI AGCT 5 cut(s) 629, 638, 988, 1145, 1335
AluI AGCT 5 cut(s) 629, 638, 988, 1145, 1335
Alw21I GWGCWC 2 cut(s) 990, 1029
Alw26I GTCTC 1 cut(s) 935
AlwI GGATC 2 cut(s) 730, 879
AoxI GGCC 2 cut(s) 240, 855
ApeKI GCWGC 4 cut(s) 518, 554, 668, 1184
AspLEI GCGC 3 cut(s) 151, 286, 1215
AsuC2I CCSGG 1 cut(s) 8
AsuHPI GGTGA 3 cut(s) 12, 25, 374
AsuII TTCGAA 3 cut(s) 81, 260, 1200
BanI GGYRCC 1 cut(s) 972
BanII GRGCYC 2 cut(s) 990, 1133
BbsI GAAGAC 1 cut(s) 716
Bbv12I GWGCWC 2 cut(s) 990, 1029
BbvI GCAGC 4 cut(s) 530, 566, 680, 1196
BccI CCATC 5 cut(s) 31, 205, 259, 469, 1174
BceAI ACGGC 1 cut(s) 255
BcgI CGANNNNNNTGC 2 cut(s) 91, 125
BciT130I CCWGG 4 cut(s) 71, 770, 1189, 1326
BciVI GTATCC 1 cut(s) 733
BcnI CCSGG 1 cut(s) 8
BcoDI GTCTC 1 cut(s) 935
BfmI CTRYAG 2 cut(s) 110, 817
BfuI GTATCC 1 cut(s) 733
BglI GCCNNNNNGGC 1 cut(s) 677
BisI GCNGC 5 cut(s) 519, 555, 669, 672, 1185
BlsI GCNGC 5 cut(s) 520, 556, 670, 673, 1186
Bme1390I CCNGG 5 cut(s) 8, 71, 770, 1189, 1326
BmiI GGNNCC 2 cut(s) 974, 1132
BmrFI CCNGG 5 cut(s) 8, 71, 770, 1189, 1326
BmsI GCATC 9 cut(s) 486, 493, 515, 853, 876, 1056, 1105, 1207, 1264
BpiI GAAGAC 1 cut(s) 716
Bpu14I TTCGAA 3 cut(s) 81, 260, 1200
BpuMI CCSGG 1 cut(s) 8
BsaAI YACGTR 1 cut(s) 1231
BsaI GGTCTC 1 cut(s) 935
BsaJI CCNNGG 4 cut(s) 336, 847, 858, 1325
BsaXI ACNNNNNCTCC 2 cut(s) 683, 713
Bsc4I CCNNNNNNNGG 1 cut(s) 853
Bse118I RCCGGY 1 cut(s) 975
Bse1I ACTGG 2 cut(s) 413, 963
Bse3DI GCAATG 4 cut(s) 322, 528, 624, 1168
BseBI CCWGG 4 cut(s) 71, 770, 1189, 1326
BseDI CCNNGG 4 cut(s) 336, 847, 858, 1325
BseGI GGATG 6 cut(s) 202, 508, 661, 868, 1071, 1276
BseLI CCNNNNNNNGG 1 cut(s) 853
BseMI GCAATG 4 cut(s) 322, 528, 624, 1168
BseMII CTCAG 5 cut(s) 462, 554, 1010, 1158, 1269
BseNI ACTGG 2 cut(s) 413, 963
BseRI GAGGAG 1 cut(s) 923
BseXI GCAGC 4 cut(s) 530, 566, 680, 1196
BseYI CCCAGC 2 cut(s) 344, 1061
Bsh1236I CGCG 6 cut(s) 101, 191, 286, 353, 1213, 1215
BshFI GGCC 2 cut(s) 242, 857
BshNI GGYRCC 1 cut(s) 972
BsiHKAI GWGCWC 2 cut(s) 990, 1029
BsiSI CCGG 4 cut(s) 7, 500, 954, 976
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 1 cut(s) 853
BsmAI GTCTC 1 cut(s) 935
BsmFI GGGAC 1 cut(s) 200
BsmI GAATGC 1 cut(s) 745
BsnI GGCC 2 cut(s) 242, 857
Bso31I GGTCTC 1 cut(s) 935
Bsp119I TTCGAA 3 cut(s) 81, 260, 1200
Bsp1286I GDGCHC 3 cut(s) 990, 1029, 1133
Bsp1407I TGTACA 1 cut(s) 641
Bsp143I GATC 3 cut(s) 735, 871, 1045
Bsp19I CCATGG 2 cut(s) 336, 847
BspACI CCGC 7 cut(s) 74, 286, 320, 437, 672, 756, 1211
BspANI GGCC 2 cut(s) 242, 857
BspCNI CTCAG 5 cut(s) 463, 553, 1009, 1159, 1270
BspFNI CGCG 6 cut(s) 101, 191, 286, 353, 1213, 1215
BspHI TCATGA 1 cut(s) 990
BspLI GGNNCC 2 cut(s) 974, 1132
BspMAI CTGCAG 1 cut(s) 114
BspPI GGATC 2 cut(s) 730, 879
BspT104I TTCGAA 3 cut(s) 81, 260, 1200
BspT107I GGYRCC 1 cut(s) 972
BspTNI GGTCTC 1 cut(s) 935
BsrDI GCAATG 4 cut(s) 322, 528, 624, 1168
BsrFI RCCGGY 1 cut(s) 975
BsrGI TGTACA 1 cut(s) 641
BsrI ACTGG 2 cut(s) 413, 963
BssAI RCCGGY 1 cut(s) 975
BssECI CCNNGG 4 cut(s) 336, 847, 858, 1325
BssMI GATC 3 cut(s) 735, 871, 1045
BssT1I CCWWGG 2 cut(s) 336, 847
Bst2UI CCWGG 4 cut(s) 71, 770, 1189, 1326
Bst4CI ACNGT 1 cut(s) 1351
Bst6I CTCTTC 2 cut(s) 46, 1202
BstAUI TGTACA 1 cut(s) 641
BstBAI YACGTR 1 cut(s) 1231
BstBI TTCGAA 3 cut(s) 81, 260, 1200
BstC8I GCNNGC 2 cut(s) 1036, 1147
BstDEI CTNAG 5 cut(s) 471, 540, 996, 1167, 1278
BstDSI CCRYGG 2 cut(s) 336, 847
BstF5I GGATG 6 cut(s) 202, 508, 661, 868, 1071, 1276
BstFNI CGCG 6 cut(s) 101, 191, 286, 353, 1213, 1215
BstHHI GCGC 3 cut(s) 151, 286, 1215
BstKTI GATC 3 cut(s) 738, 874, 1048
BstMAI GTCTC 1 cut(s) 935
BstMBI GATC 3 cut(s) 735, 871, 1045
BstNI CCWGG 4 cut(s) 71, 770, 1189, 1326
BstNSI RCATGY 2 cut(s) 689, 945
BstSCI CCNGG 5 cut(s) 6, 69, 768, 1187, 1324
BstSFI CTRYAG 2 cut(s) 110, 817
BstUI CGCG 6 cut(s) 101, 191, 286, 353, 1213, 1215
BstV1I GCAGC 4 cut(s) 530, 566, 680, 1196
BstV2I GAAGAC 1 cut(s) 716
BstX2I RGATCY 1 cut(s) 871
BstXI CCANNNNNNTGG 1 cut(s) 420
BstYI RGATCY 1 cut(s) 871
BsuI GTATCC 1 cut(s) 733
BsuRI GGCC 2 cut(s) 242, 857
BtgI CCRYGG 2 cut(s) 336, 847
BtgZI GCGATG 1 cut(s) 1230
BtsCI GGATG 6 cut(s) 202, 508, 661, 868, 1071, 1276
BtsI GCAGTG 3 cut(s) 119, 317, 831
BtsIMutI CAGTG 5 cut(s) 119, 317, 420, 613, 831
Cac8I GCNNGC 2 cut(s) 1036, 1147
CciI TCATGA 1 cut(s) 990
CfoI GCGC 3 cut(s) 151, 286, 1215
Cfr10I RCCGGY 1 cut(s) 975
CseI GACGC 1 cut(s) 197
Csp6I GTAC 3 cut(s) 349, 388, 642
CspCI CAANNNNNGTGG 4 cut(s) 40, 75, 669, 704
CviQI GTAC 3 cut(s) 349, 388, 642
DdeI CTNAG 5 cut(s) 471, 540, 996, 1167, 1278
DpnI GATC 3 cut(s) 737, 873, 1047
DpnII GATC 3 cut(s) 735, 871, 1045
EaeI YGGCCR 1 cut(s) 240
Eam1104I CTCTTC 2 cut(s) 46, 1202
EarI CTCTTC 2 cut(s) 46, 1202
Ecl136II GAGCTC 1 cut(s) 988
Eco130I CCWWGG 2 cut(s) 336, 847
Eco24I GRGCYC 2 cut(s) 990, 1133
Eco31I GGTCTC 1 cut(s) 935
Eco53kI GAGCTC 1 cut(s) 988
EcoICRI GAGCTC 1 cut(s) 988
EcoRII CCWGG 4 cut(s) 69, 768, 1187, 1324
EcoT14I CCWWGG 2 cut(s) 336, 847
EcoT22I ATGCAT 4 cut(s) 508, 733, 747, 868
EcoT38I GRGCYC 2 cut(s) 990, 1133
ErhI CCWWGG 2 cut(s) 336, 847
FalI AAGNNNNNCTT 2 cut(s) 701, 733
FaqI GGGAC 1 cut(s) 200
FauI CCCGC 1 cut(s) 430
FauNDI CATATG 2 cut(s) 159, 741
Fnu4HI GCNGC 5 cut(s) 519, 555, 669, 672, 1185
FokI GGATG 6 cut(s) 209, 515, 668, 875, 1078, 1283
FriOI GRGCYC 2 cut(s) 990, 1133
Fsp4HI GCNGC 5 cut(s) 519, 555, 669, 672, 1185
GlaI GCGC 3 cut(s) 150, 285, 1214
GluI GCNGC 5 cut(s) 519, 555, 669, 672, 1185
GsaI CCCAGC 2 cut(s) 348, 1065
HaeIII GGCC 2 cut(s) 242, 857
HapII CCGG 4 cut(s) 7, 500, 954, 976
HgaI GACGC 1 cut(s) 197
HhaI GCGC 3 cut(s) 151, 286, 1215
Hin6I GCGC 3 cut(s) 149, 284, 1213
HinP1I GCGC 3 cut(s) 149, 284, 1213
HinfI GANTC 5 cut(s) 206, 603, 994, 1300, 1343
HpaII CCGG 4 cut(s) 7, 500, 954, 976
HphI GGTGA 3 cut(s) 12, 25, 374
Hpy166II GTNNAC 3 cut(s) 33, 97, 644
Hpy188I TCNGA 1 cut(s) 543
Hpy188III TCNNGA 2 cut(s) 880, 991
Hpy8I GTNNAC 3 cut(s) 33, 97, 644
Hpy99I CGWCG 1 cut(s) 929
HpyAV CCTTC 5 cut(s) 46, 77, 689, 890, 1050
HpyCH4III ACNGT 1 cut(s) 1351
HpyCH4IV ACGT 1 cut(s) 1230
HpyF3I CTNAG 5 cut(s) 471, 540, 996, 1167, 1278
HpySE526I ACGT 1 cut(s) 1230
HspAI GCGC 3 cut(s) 149, 284, 1213
Kzo9I GATC 3 cut(s) 735, 871, 1045
LmnI GCTCC 4 cut(s) 626, 1024, 1136, 1142
Lsp1109I GCAGC 4 cut(s) 530, 566, 680, 1196
LweI GCATC 9 cut(s) 486, 493, 515, 853, 876, 1056, 1105, 1207, 1264
MaeII ACGT 1 cut(s) 1230
MaeIII GTNAC 2 cut(s) 829, 916
MalI GATC 3 cut(s) 737, 873, 1047
MboI GATC 3 cut(s) 735, 871, 1045
MboII GAAGA 8 cut(s) 33, 146, 227, 274, 721, 895, 1040, 1189
MflI RGATCY 1 cut(s) 871
MhlI GDGCHC 3 cut(s) 990, 1029, 1133
MluCI AATT 3 cut(s) 383, 663, 1225
MlyI GAGTC 2 cut(s) 612, 988
Mph1103I ATGCAT 4 cut(s) 508, 733, 747, 868
MseI TTAA 1 cut(s) 660
MslI CAYNNNNRTG 3 cut(s) 162, 569, 1161
MspI CCGG 4 cut(s) 7, 500, 954, 976
MspR9I CCNGG 5 cut(s) 8, 71, 770, 1189, 1326
Mva1269I GAATGC 1 cut(s) 745
MvaI CCWGG 4 cut(s) 71, 770, 1189, 1326
MvnI CGCG 6 cut(s) 101, 191, 286, 353, 1213, 1215
NciI CCSGG 1 cut(s) 8
NcoI CCATGG 2 cut(s) 336, 847
NdeI CATATG 2 cut(s) 159, 741
NdeII GATC 3 cut(s) 735, 871, 1045
NlaIV GGNNCC 2 cut(s) 974, 1132
NmeAIII GCCGAG 1 cut(s) 883
NmuCI GTSAC 1 cut(s) 829
NsiI ATGCAT 4 cut(s) 508, 733, 747, 868
NspI RCATGY 2 cut(s) 689, 945
NspV TTCGAA 3 cut(s) 81, 260, 1200
PagI TCATGA 1 cut(s) 990
PcsI WCGNNNNNNNCGW 1 cut(s) 1110
PctI GAATGC 1 cut(s) 745
PfeI GAWTC 3 cut(s) 206, 1300, 1343
PflFI GACNNNGTC 1 cut(s) 1100
PkrI GCNGC 5 cut(s) 520, 556, 670, 673, 1186
PleI GAGTC 2 cut(s) 611, 988
PpsI GAGTC 2 cut(s) 611, 988
Ppu21I YACGTR 1 cut(s) 1231
PsiI TTATAA 1 cut(s) 1091
Psp124BI GAGCTC 1 cut(s) 990
Psp6I CCWGG 4 cut(s) 69, 768, 1187, 1324
PspFI CCCAGC 2 cut(s) 344, 1061
PspGI CCWGG 4 cut(s) 69, 768, 1187, 1324
PspN4I GGNNCC 2 cut(s) 974, 1132
PstI CTGCAG 1 cut(s) 114
PsuI RGATCY 1 cut(s) 871
PsyI GACNNNGTC 1 cut(s) 1100
RsaI GTAC 3 cut(s) 350, 389, 643
RsaNI GTAC 3 cut(s) 349, 388, 642
RseI CAYNNNNRTG 3 cut(s) 162, 569, 1161
SacI GAGCTC 1 cut(s) 990
SaqAI TTAA 1 cut(s) 660
SatI GCNGC 5 cut(s) 519, 555, 669, 672, 1185
Sau3AI GATC 3 cut(s) 735, 871, 1045
SchI GAGTC 2 cut(s) 612, 988
ScrFI CCNGG 5 cut(s) 8, 71, 770, 1189, 1326
SduI GDGCHC 3 cut(s) 990, 1029, 1133
SfaNI GCATC 9 cut(s) 486, 493, 515, 853, 876, 1056, 1105, 1207, 1264
SfcI CTRYAG 2 cut(s) 110, 817
SfuI TTCGAA 3 cut(s) 81, 260, 1200
SmiMI CAYNNNNRTG 3 cut(s) 162, 569, 1161
Sse9I AATT 3 cut(s) 383, 663, 1225
SsiI CCGC 7 cut(s) 74, 286, 320, 437, 672, 756, 1211
SstI GAGCTC 1 cut(s) 990
StyD4I CCNGG 5 cut(s) 6, 69, 768, 1187, 1324
StyI CCWWGG 2 cut(s) 336, 847
TaaI ACNGT 1 cut(s) 1351
TaiI ACGT 1 cut(s) 1233
TaqI TCGA 5 cut(s) 81, 209, 260, 881, 1200
TaqII GACCGA 2 cut(s) 917, 922
TasI AATT 3 cut(s) 383, 663, 1225
TatI WGTACW 1 cut(s) 641
TauI GCSGC 1 cut(s) 674
TfiI GAWTC 3 cut(s) 206, 1300, 1343
Tru1I TTAA 1 cut(s) 660
Tru9I TTAA 1 cut(s) 660
TscAI CASTG 5 cut(s) 119, 317, 420, 613, 838
TseFI GTSAC 1 cut(s) 829
TseI GCWGC 4 cut(s) 518, 554, 668, 1184
Tsp45I GTSAC 1 cut(s) 829
TspDTI ATGAA 3 cut(s) 146, 1145, 1313
TspGWI ACGGA 1 cut(s) 34
TspRI CASTG 5 cut(s) 119, 317, 420, 613, 838
Tth111I GACNNNGTC 1 cut(s) 1100
XceI RCATGY 2 cut(s) 689, 945
Zsp2I ATGCAT 4 cut(s) 508, 733, 747, 868
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.