MD09G1185300.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0022348
Physical Location & Seq
Reverse (-)
16037860 .. 16039134
1275 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1185300.v1.1.491

Sequence Viewer

Length: 522 bp
ATGTATAAGAAAATGAGGAAAGCAAAAGTGAGAGTCAGTGAGGTGGCAATGGTGCGAGCTATTTCAGCTTGTACACAACTTGGGGAAGTTGAAATCGCAGCCACATTGGCAAGGCATGTGGAGGAAGGGTTTTGTGAGAGGACAATCTTTGTATCCAATGCATTGATCCATATACATTCCAAATGTGGAAACATAGACCAGGCATGGAGAGAGTTCAACAGAATGAGCATGAGGGATGTCATATCCTATAGCGCGTTAATCACTGCCCTCGCTGACCATGGAAAGGCCAAGGATGCGTTGGATCTTTTCTCGAAGATGCAAAAGGAAGGAGTTAGACCGAACCAGGTAACATTCGTCGGTCTCCTCAATGCATGTAGCCATGCCGGACTGATTGAGAGAGGGTGCCAGTACTTTGAGCTCATGGCTCAGGTTTTCGGCACTGAGCCACTAAAGGAGCACTATGCTTGCATGGTTGATCTTCTTGGAAGGGCTAAATGCTTGAAAAGGCATACAATCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

174

Amino Acids

19.45

Weight (kDa)

9.01

Isoelectric Point (pI)

45.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 78 - 126 3.6e-14 PPR repeat family
PPR_3 PF13812 79 - 125 2e-10 Pentatricopeptide repeat domain
PPR PF01535 81 - 111 3.9e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022348)

Species Orthologous Gene IDs
malus_domestica MD09G1185000.v1.1 MD09G1185300.v1.1
prunus_persica Prupe.3G043100_v2.0.a1
pyrus_communis pycom09g10260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 520
AccB1I GGYRCC 1 cut(s) 402
AccII CGCG 1 cut(s) 254
AclWI GGATC 2 cut(s) 160, 309
AfaI GTAC 2 cut(s) 73, 410
AfiI CCNNNNNNNGG 1 cut(s) 283
AgsI TTSAA 3 cut(s) 92, 217, 502
AjnI CCWGG 2 cut(s) 198, 342
AluBI AGCT 3 cut(s) 59, 68, 418
AluI AGCT 3 cut(s) 59, 68, 418
Alw21I GWGCWC 2 cut(s) 420, 459
Alw26I GTCTC 1 cut(s) 365
AlwI GGATC 2 cut(s) 160, 309
AoxI GGCC 1 cut(s) 285
ApeKI GCWGC 1 cut(s) 98
AspLEI GCGC 1 cut(s) 254
BanI GGYRCC 1 cut(s) 402
BanII GRGCYC 1 cut(s) 420
Bbv12I GWGCWC 2 cut(s) 420, 459
BbvI GCAGC 1 cut(s) 110
BciT130I CCWGG 2 cut(s) 200, 344
BciVI GTATCC 1 cut(s) 163
BcoDI GTCTC 1 cut(s) 365
BfmI CTRYAG 1 cut(s) 247
BfuI GTATCC 1 cut(s) 163
BglI GCCNNNNNGGC 1 cut(s) 107
BisI GCNGC 1 cut(s) 99
BlsI GCNGC 1 cut(s) 100
BmcAI AGTACT 1 cut(s) 410
Bme1390I CCNGG 2 cut(s) 200, 344
BmiI GGNNCC 1 cut(s) 404
BmrFI CCNGG 2 cut(s) 200, 344
BmsI GCATC 2 cut(s) 283, 306
Bpu10I CCTNAGC 1 cut(s) 426
BsaI GGTCTC 1 cut(s) 365
BsaJI CCNNGG 2 cut(s) 277, 288
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 1 cut(s) 283
Bse1I ACTGG 1 cut(s) 406
Bse3DI GCAATG 1 cut(s) 54
BseBI CCWGG 2 cut(s) 200, 344
BseDI CCNNGG 2 cut(s) 277, 288
BseGI GGATG 2 cut(s) 241, 298
BseLI CCNNNNNNNGG 1 cut(s) 283
BseMI GCAATG 1 cut(s) 54
BseMII CTCAG 2 cut(s) 432, 440
BseNI ACTGG 1 cut(s) 406
BseRI GAGGAG 1 cut(s) 353
BseXI GCAGC 1 cut(s) 110
Bsh1236I CGCG 1 cut(s) 254
BshFI GGCC 1 cut(s) 287
BshNI GGYRCC 1 cut(s) 402
BsiHKAI GWGCWC 2 cut(s) 420, 459
BsiSI CCGG 1 cut(s) 384
BslI CCNNNNNNNGG 1 cut(s) 283
BsmAI GTCTC 1 cut(s) 365
BsnI GGCC 1 cut(s) 287
Bso31I GGTCTC 1 cut(s) 365
Bsp1286I GDGCHC 2 cut(s) 420, 459
Bsp1407I TGTACA 1 cut(s) 71
Bsp143I GATC 3 cut(s) 165, 301, 475
Bsp19I CCATGG 1 cut(s) 277
BspANI GGCC 1 cut(s) 287
BspCNI CTCAG 2 cut(s) 433, 439
BspFNI CGCG 1 cut(s) 254
BspLI GGNNCC 1 cut(s) 404
BspPI GGATC 2 cut(s) 160, 309
BspT107I GGYRCC 1 cut(s) 402
BspTNI GGTCTC 1 cut(s) 365
BsrDI GCAATG 1 cut(s) 54
BsrGI TGTACA 1 cut(s) 71
BsrI ACTGG 1 cut(s) 406
BssECI CCNNGG 2 cut(s) 277, 288
BssMI GATC 3 cut(s) 165, 301, 475
BssT1I CCWWGG 2 cut(s) 277, 288
Bst2UI CCWGG 2 cut(s) 200, 344
BstAUI TGTACA 1 cut(s) 71
BstC8I GCNNGC 2 cut(s) 57, 466
BstDEI CTNAG 2 cut(s) 426, 441
BstDSI CCRYGG 1 cut(s) 277
BstF5I GGATG 2 cut(s) 241, 298
BstFNI CGCG 1 cut(s) 254
BstHHI GCGC 1 cut(s) 254
BstKTI GATC 3 cut(s) 168, 304, 478
BstMAI GTCTC 1 cut(s) 365
BstMBI GATC 3 cut(s) 165, 301, 475
BstMWI GCNNNNNNNGC 3 cut(s) 65, 107, 293
BstNI CCWGG 2 cut(s) 200, 344
BstNSI RCATGY 2 cut(s) 119, 375
BstSCI CCNGG 2 cut(s) 198, 342
BstSFI CTRYAG 1 cut(s) 247
BstUI CGCG 1 cut(s) 254
BstV1I GCAGC 1 cut(s) 110
BstX2I RGATCY 1 cut(s) 301
BstYI RGATCY 1 cut(s) 301
BsuI GTATCC 1 cut(s) 163
BsuRI GGCC 1 cut(s) 287
BtgI CCRYGG 1 cut(s) 277
BtsCI GGATG 2 cut(s) 241, 298
BtsI GCAGTG 1 cut(s) 261
BtsIMutI CAGTG 3 cut(s) 43, 261, 438
Cac8I GCNNGC 2 cut(s) 57, 466
CfoI GCGC 1 cut(s) 254
CsiI ACCWGGT 1 cut(s) 342
Csp6I GTAC 2 cut(s) 72, 409
CspCI CAANNNNNGTGG 2 cut(s) 99, 134
CviAII CATG 8 cut(s) 116, 204, 229, 278, 372, 380, 421, 469
CviJI RGCY 9 cut(s) 59, 68, 101, 287, 378, 418, 425, 445, 491
CviKI_1 RGCY 9 cut(s) 59, 68, 101, 287, 378, 418, 425, 445, 491
CviQI GTAC 2 cut(s) 72, 409
DdeI CTNAG 2 cut(s) 426, 441
DpnI GATC 3 cut(s) 167, 303, 477
DpnII GATC 3 cut(s) 165, 301, 475
Ecl136II GAGCTC 1 cut(s) 418
Eco130I CCWWGG 2 cut(s) 277, 288
Eco24I GRGCYC 1 cut(s) 420
Eco31I GGTCTC 1 cut(s) 365
Eco53kI GAGCTC 1 cut(s) 418
EcoICRI GAGCTC 1 cut(s) 418
EcoRII CCWGG 2 cut(s) 198, 342
EcoT14I CCWWGG 2 cut(s) 277, 288
EcoT22I ATGCAT 2 cut(s) 163, 373
EcoT38I GRGCYC 1 cut(s) 420
ErhI CCWWGG 2 cut(s) 277, 288
FaeI CATG 8 cut(s) 119, 207, 232, 281, 375, 383, 424, 472
FatI CATG 8 cut(s) 115, 203, 228, 277, 371, 379, 420, 468
Fnu4HI GCNGC 1 cut(s) 99
FokI GGATG 2 cut(s) 248, 305
FriOI GRGCYC 1 cut(s) 420
Fsp4HI GCNGC 1 cut(s) 99
GlaI GCGC 1 cut(s) 253
GluI GCNGC 1 cut(s) 99
HaeIII GGCC 1 cut(s) 287
HapII CCGG 1 cut(s) 384
HhaI GCGC 1 cut(s) 254
Hin1II CATG 8 cut(s) 119, 207, 232, 281, 375, 383, 424, 472
Hin6I GCGC 1 cut(s) 252
HinP1I GCGC 1 cut(s) 252
HinfI GANTC 1 cut(s) 33
HpaII CCGG 1 cut(s) 384
Hpy166II GTNNAC 1 cut(s) 74
Hpy188III TCNNGA 1 cut(s) 310
Hpy8I GTNNAC 1 cut(s) 74
Hpy99I CGWCG 1 cut(s) 359
HpyAV CCTTC 3 cut(s) 119, 320, 480
HpyCH4V TGCA 4 cut(s) 161, 319, 371, 468
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 107, 293
HpyF3I CTNAG 2 cut(s) 426, 441
Hsp92II CATG 8 cut(s) 119, 207, 232, 281, 375, 383, 424, 472
HspAI GCGC 1 cut(s) 252
Kzo9I GATC 3 cut(s) 165, 301, 475
LmnI GCTCC 1 cut(s) 454
LpnPI CCDG 7 cut(s) 185, 212, 329, 356, 397, 413, 419
Lsp1109I GCAGC 1 cut(s) 110
LweI GCATC 2 cut(s) 283, 306
MabI ACCWGGT 1 cut(s) 342
MaeIII GTNAC 1 cut(s) 346
MalI GATC 3 cut(s) 167, 303, 477
MboI GATC 3 cut(s) 165, 301, 475
MboII GAAGA 2 cut(s) 325, 470
MflI RGATCY 1 cut(s) 301
MhlI GDGCHC 2 cut(s) 420, 459
MlyI GAGTC 1 cut(s) 42
MmeI TCCRAC 1 cut(s) 279
MnlI CCTC 8 cut(s) 9, 34, 115, 132, 225, 278, 374, 392
Mph1103I ATGCAT 2 cut(s) 163, 373
MseI TTAA 1 cut(s) 257
MspI CCGG 1 cut(s) 384
MspR9I CCNGG 2 cut(s) 200, 344
MvaI CCWGG 2 cut(s) 200, 344
MvnI CGCG 1 cut(s) 254
MwoI GCNNNNNNNGC 3 cut(s) 65, 107, 293
NcoI CCATGG 1 cut(s) 277
NdeII GATC 3 cut(s) 165, 301, 475
NlaIII CATG 8 cut(s) 119, 207, 232, 281, 375, 383, 424, 472
NlaIV GGNNCC 1 cut(s) 404
NsiI ATGCAT 2 cut(s) 163, 373
NspI RCATGY 2 cut(s) 119, 375
PkrI GCNGC 1 cut(s) 100
PleI GAGTC 1 cut(s) 41
PpsI GAGTC 1 cut(s) 41
PsiI TTATAA 1 cut(s) 520
Psp124BI GAGCTC 1 cut(s) 420
Psp6I CCWGG 2 cut(s) 198, 342
PspGI CCWGG 2 cut(s) 198, 342
PspN4I GGNNCC 1 cut(s) 404
PsuI RGATCY 1 cut(s) 301
RsaI GTAC 2 cut(s) 73, 410
RsaNI GTAC 2 cut(s) 72, 409
SacI GAGCTC 1 cut(s) 420
SaqAI TTAA 1 cut(s) 257
SatI GCNGC 1 cut(s) 99
Sau3AI GATC 3 cut(s) 165, 301, 475
ScaI AGTACT 1 cut(s) 410
SchI GAGTC 1 cut(s) 42
ScrFI CCNGG 2 cut(s) 200, 344
SduI GDGCHC 2 cut(s) 420, 459
SetI ASST 6 cut(s) 45, 61, 70, 348, 420, 432
SexAI ACCWGGT 1 cut(s) 342
SfaNI GCATC 2 cut(s) 283, 306
SfcI CTRYAG 1 cut(s) 247
SstI GAGCTC 1 cut(s) 420
StyD4I CCNGG 2 cut(s) 198, 342
StyI CCWWGG 2 cut(s) 277, 288
TaqI TCGA 1 cut(s) 311
TaqII GACCGA 2 cut(s) 347, 352
TatI WGTACW 2 cut(s) 71, 408
Tru1I TTAA 1 cut(s) 257
Tru9I TTAA 1 cut(s) 257
TscAI CASTG 3 cut(s) 43, 268, 445
TseI GCWGC 1 cut(s) 98
TspRI CASTG 3 cut(s) 43, 268, 445
XceI RCATGY 2 cut(s) 119, 375
XcmI CCANNNNNNNNNTGG 1 cut(s) 295
ZrmI AGTACT 1 cut(s) 410
Zsp2I ATGCAT 2 cut(s) 163, 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.