MD09G1205400.v1.1

Homeobox-leucine zipper protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014924
Physical Location & Seq
Reverse (-)
19577406 .. 19584311
6906 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1205400.v1.1.491

Sequence Viewer

Length: 2517 bp
ATGGCTATGTCCTGCAAGGATGGTAATAAGCATGCATTGGATAATGGCAAGTATGTGAGGTACACACCTGAGCAGGTTGAAGCCCTCGAGAGGCTTTATCATGATTGCCCCAAACCCAGTTCGATTCGCCGCCAACAGCTTATTCGGGAGTGCCCGATTCTCTCCAACATCGAACCGAAACAGATTAAGGTCTGGTTCCAGAACAGAAGATGTAGAGAGAAGCAGAGGAAAGAGGCTTCACGCCTCCAAGCTGTCAACAGGAAGCTCAGTGCTATGAATAAGCTACTAATGGAGGAAAATGATAGGTTGCAGAAGCAGGTGTCTCACTTGGTGTATGAAAATGGTTACTTCCGTCAGCATACCCAGGGGACAACGCTTGCAACCAAAGACACGAGTTGTGAATCAGTGGTGACGAGTGGCCAACACCATTTGACACCTCAGCATCCGCCAAGGGATGCAAGTCCTGCAGGACTTTTGTCCATTGCAGAAGAAACTTTAGCAGAGTTTCTTTCAAAGGCAACTGGAACTGCTGTTGAGTGGGTCCAAATGCCTGGAATGAAGCCTGGTCCGGATTCCATTGGAATCGTTGCTATTTCTCATGGTTGCACTGGAGTGGCAGCACGAGCCTGCGGCCTGGTGGGTCTAGAGCCTACCAGGGTTGCGGAGATCCTCAAAGATCTGCCGTCGTGGTTGCGTGATTGCCGCGCTGTAGATGTTTTAAATGTATTGCCTACAGCTAACGGTGGAACCATTGAGCTGCTCTACATGCAGCTATATGCGCCGACAACTTTGGCGCCTGCTTGCGATTTCTGGCTACTGCGCTATACGTCTGTTTTAGAGGATGGCAGCCTTGTGGTATGTGCGAGATCTCTGAAAAACACTCAGAATGGTCCAACCATGCCTCCAGTGCAGCACTTTGTCAGAGCAGAAATGCTGCCTAGTGGATACCTTATACGACCCTGTGAAGGGGGTGGCTCGATTATACATATCGTTGATCATATGGATTTAGAGCCTTGTAGCGTGCCTGAAGTCCTGCGCCCATTGTATGAATCATCTGCTGTTCTTGCTCAAAAGATGACAATGGCGGCTTTACGTCAGCTGAGACAGATAGCGCATGAGGTTTCTCAATCTAATGTCACAGGTTGGGGAAGACGACCTGCAGCTTTACGAGCATTAAGCCAGAGGCTTAGCAGGGGTTTTAATGATGCACTTAATGGATTTACCGATGAGGGTTGGTCCATGATGGGAAATGATGGCATGGATGATGTTACTATCCTCATAAACTCGTCTCCTGACAAGCTGATGGGCTTAAACCTGTCCTTTGGTAATGGATTCCCAGCTGTCAGTAATTCTGTCTTATGTGCTAAAGCATCCATGCTGTTACAGAATGTGCCCCCTGCTATCCTTCTTCGGTTCCTGCGCGAGCACAGATCAGAATGGGCAGACAACAATATTGATGCTTACTCAGCTGCAGCTGTTAAAGTCGGTCCTTGTAGCTTAGCTGGGTCTCGAGTTGGTAGTTTTGGAGGTCAAGTTATACTCCCTCTGGCTCACACTCTTGAGCATGAAGAGTTCTTGGAAGTCATTAAATTGGAAGGCGTTGGCCATTCTCCTGAAGATGCAATGATGCCAAGAGAAATGTTTCTCTTGCAACTATGCAGTGGAATGGACGAGAATGCTGTGGGTTCCTGTGCTGAACTCATTTTTGCTCCAATTGATGCTTCTTTTGCTGATGATGCACCCCTTCTACCTTCTGGGTTTCGCATCATTCCTCTTGATTATGGGAAGGAAGCTTCCAGTCCAAATCGCACCTTGGACCTTGCATCTGCTCTTGAAATTGGGCCAACTGGAAATAAAGGATCTAGTGAATATTCTGCAAGTGCTGGTTGTGTGAGATCTGTCATGACTATAGCATTTGAATTTGCTTGTGAGACCCACATGCAAGAGCATGTAGCATCCATGGCCCGACAATATGTTCGCAGCATTATATCTTCAGTTCAGAGGGTGGCATTAGCTCTCTCTCCTTCAAATTTGAGTTCACAGGCTGGTCTTCGGTCACCACTTGGTACTCCTGAAGCGCAAACACTTGCTCGTTGGATCTGCAACAGTTACAGGTGCTATCTGGGTGTGGAGCTGCTCAAATCGGGCAATGAAGGAAGTGAATCCATCCTCAAATCTTTGTGGCATCACTCCGATGCAATTATGTGCTGCTCTCTGAAGGCATTGCCAGTTTTCACCTTTGCAAACCAGGCAGGGCTTGACATGCTGGAGACCACCTTGGTTGCGCTGCAAGACATAACTTTGGAGAAGATATTTGATGACCATGGACGAAAGACTCTGTGCTCGGAATTCCCCCAAATAATGCAACAGGGTTTCACTTGTCTTCAAGGTGGCATCTGTCTTTCGAGCATGGGGCGACCAGTGTCATATGAGAGAGCAGTGGCGTGGAAGGTGTTGAACGAAGAGGAGACGGCTCACTGCATGTGTTTCTTGTTTGTAAACTGGTCTTTCGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Homeobox MEKHLA START

Protein Analysis

839

Amino Acids

91.88

Weight (kDa)

6.03

Isoelectric Point (pI)

48.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 17 - 75 2.6e-15 Homeodomain
START PF01852 161 - 369 8.7e-51 START domain
MEKHLA PF08670 695 - 837 3e-48 MEKHLA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014924)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52150 AT1G52150 AT1G52150
fragaria_vesca FvH4_6g32240
malus_domestica MD09G1205400.v1.1 MD17G1185400.v1.1
prunus_persica Prupe.3G060700_v2.0.a1
pyrus_communis pycom09g12200 pycom17g19500
rosa_chinensis RchiOBHm_Chr2g0140131
rosa_laevigata RLG00000019854
rosa_roxburghii Rroxscaffold_2G00105020
rosa_rugosa Rorug02G0361400
rosa_samantha Rh2BG422400 Rh2CG398100
rosa_wichuraiana Rw2G033760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 307
Acc36I ACCTGC 3 cut(s) 64, 307, 1165
AccB1I GGYRCC 1 cut(s) 793
AccII CGCG 2 cut(s) 705, 1422
AccIII TCCGGA 1 cut(s) 568
AciI CCGC 6 cut(s) 130, 446, 630, 662, 703, 1085
AclWI GGATC 3 cut(s) 661, 1867, 2105
AcoI YGGCCR 2 cut(s) 418, 1603
AcsI RAATTY 3 cut(s) 1919, 2029, 2348
AcuI CTGAAG 5 cut(s) 1047, 1635, 1977, 2094, 2237
AcyI GRCGYC 1 cut(s) 794
AdeI CACNNNGTG 1 cut(s) 331
AfaI GTAC 2 cut(s) 62, 2068
AfiI CCNNNNNNNGG 3 cut(s) 90, 965, 966
AgsI TTSAA 7 cut(s) 80, 513, 1835, 1919, 2028, 2387, 2458
AjnI CCWGG 6 cut(s) 363, 550, 562, 633, 653, 2247
AjuI GAANNNNNNNTTGG 4 cut(s) 126, 158, 1705, 1737
AleI CACNNNNGTG 1 cut(s) 611
Alw21I GWGCWC 2 cut(s) 1428, 2345
Alw26I GTCTC 7 cut(s) 327, 1096, 1293, 1512, 1925, 2264, 2462
AlwI GGATC 3 cut(s) 661, 1867, 2105
Ama87I CYCGRG 2 cut(s) 86, 1509
Aor13HI TCCGGA 1 cut(s) 568
AoxI GGCC 5 cut(s) 418, 631, 1603, 1841, 1962
ApoI RAATTY 3 cut(s) 1919, 2029, 2348
ArsI GACNNNNNNTTYG 2 cut(s) 1959, 1991
Asp700I GAANNNNTTC 1 cut(s) 1641
AspLEI GCGC 9 cut(s) 707, 781, 796, 822, 1038, 1114, 1422, 2080, 2287
AspS9I GGNCC 8 cut(s) 541, 566, 890, 1236, 1487, 1816, 1841, 1963
AsuHPI GGTGA 3 cut(s) 421, 2049, 2227
AvaI CYCGRG 2 cut(s) 86, 1509
AvaII GGWCC 6 cut(s) 541, 566, 890, 1236, 1487, 1816
BaeGI GKGCMC 2 cut(s) 155, 1395
BalI TGGCCA 2 cut(s) 420, 1605
BanI GGYRCC 1 cut(s) 793
BauI CACGAG 2 cut(s) 391, 621
BbsI GAAGAC 3 cut(s) 1156, 2042, 2375
Bbv12I GWGCWC 2 cut(s) 1428, 2345
BbvCI CCTCAGC 1 cut(s) 438
BccI CCATC 6 cut(s) 14, 836, 1237, 1247, 1297, 2174
BceAI ACGGC 2 cut(s) 667, 2487
BciT130I CCWGG 6 cut(s) 365, 552, 564, 635, 655, 2249
BciVI GTATCC 1 cut(s) 938
BclI TGATCA 1 cut(s) 994
BcoDI GTCTC 7 cut(s) 327, 1096, 1293, 1512, 1925, 2264, 2462
BfaI CTAG 3 cut(s) 644, 939, 1863
BfmI CTRYAG 6 cut(s) 465, 708, 732, 1158, 1470, 1908
BfoI RGCGCY 1 cut(s) 797
BfuAI ACCTGC 3 cut(s) 64, 307, 1165
BfuI GTATCC 1 cut(s) 938
BglII AGATCT 3 cut(s) 676, 866, 1895
BlpI GCTNAGC 2 cut(s) 1187, 1498
Bme1390I CCNGG 6 cut(s) 365, 552, 564, 635, 655, 2249
Bme18I GGWCC 6 cut(s) 541, 566, 890, 1236, 1487, 1816
BmeT110I CYCGRG 2 cut(s) 86, 1509
BmgT120I GGNCC 8 cut(s) 541, 566, 890, 1236, 1487, 1816, 1841, 1963
BmiI GGNNCC 6 cut(s) 197, 542, 748, 795, 1415, 1687
BmrFI CCNGG 6 cut(s) 365, 552, 564, 635, 655, 2249
BmrI ACTGGG 1 cut(s) 111
BmuI ACTGGG 1 cut(s) 111
BoxI GACNNNNGTC 2 cut(s) 475, 2422
BpiI GAAGAC 3 cut(s) 1156, 2042, 2375
BpmI CTGGAG 3 cut(s) 630, 888, 2288
Bpu10I CCTNAGC 2 cut(s) 69, 438
Bpu1102I GCTNAGC 2 cut(s) 1187, 1498
BpuEI CTTGAG 1 cut(s) 1580
BsaHI GRCGYC 1 cut(s) 794
BsaI GGTCTC 3 cut(s) 1512, 1925, 2264
BsaJI CCNNGG 8 cut(s) 363, 364, 449, 654, 1812, 1959, 2277, 2323
BsaWI WCCGGW 1 cut(s) 568
BsaXI ACNNNNNCTCC 4 cut(s) 886, 916, 1518, 1548
Bsc4I CCNNNNNNNGG 3 cut(s) 90, 965, 966
Bse1I ACTGG 9 cut(s) 117, 526, 613, 905, 1797, 1852, 2228, 2420, 2507
Bse3DI GCAATG 4 cut(s) 480, 1629, 2155, 2222
BseAI TCCGGA 1 cut(s) 568
BseBI CCWGG 6 cut(s) 365, 552, 564, 635, 655, 2249
BseDI CCNNGG 8 cut(s) 363, 364, 449, 654, 1812, 1959, 2277, 2323
BseGI GGATG 8 cut(s) 25, 442, 460, 847, 1267, 1370, 1955, 2166
BseLI CCNNNNNNNGG 3 cut(s) 90, 965, 966
BseMI GCAATG 4 cut(s) 480, 1629, 2155, 2222
BseMII CTCAG 6 cut(s) 60, 280, 452, 896, 1091, 1479
BseNI ACTGG 9 cut(s) 117, 526, 613, 905, 1797, 1852, 2228, 2420, 2507
BseRI GAGGAG 1 cut(s) 2480
BseSI GKGCMC 2 cut(s) 155, 1395
BseYI CCCAGC 2 cut(s) 1336, 1502
BsgI GTGCAG 1 cut(s) 929
Bsh1236I CGCG 2 cut(s) 705, 1422
BshFI GGCC 5 cut(s) 420, 633, 1605, 1843, 1964
BshNI GGYRCC 1 cut(s) 793
BsiHKAI GWGCWC 2 cut(s) 1428, 2345
BsiHKCI CYCGRG 2 cut(s) 86, 1509
BsiSI CCGG 1 cut(s) 569
BslFI GGGAC 1 cut(s) 382
BslI CCNNNNNNNGG 3 cut(s) 90, 965, 966
BsmAI GTCTC 7 cut(s) 327, 1096, 1293, 1512, 1925, 2264, 2462
BsmBI CGTCTC 2 cut(s) 1293, 2462
BsmFI GGGAC 1 cut(s) 382
BsmI GAATGC 1 cut(s) 1681
BsnI GGCC 5 cut(s) 420, 633, 1605, 1843, 1964
Bso31I GGTCTC 3 cut(s) 1512, 1925, 2264
BsoBI CYCGRG 2 cut(s) 86, 1509
Bsp1286I GDGCHC 4 cut(s) 155, 1395, 1428, 2345
Bsp13I TCCGGA 1 cut(s) 568
Bsp143I GATC 8 cut(s) 666, 676, 866, 994, 1430, 1859, 1895, 2097
Bsp1720I GCTNAGC 2 cut(s) 1187, 1498
Bsp19I CCATGG 2 cut(s) 1959, 2323
BspACI CCGC 6 cut(s) 130, 446, 630, 662, 703, 1085
BspANI GGCC 5 cut(s) 420, 633, 1605, 1843, 1964
BspCNI CTCAG 6 cut(s) 61, 279, 451, 895, 1092, 1478
BspEI TCCGGA 1 cut(s) 568
BspFNI CGCG 2 cut(s) 705, 1422
BspHI TCATGA 2 cut(s) 100, 1902
BspLI GGNNCC 6 cut(s) 197, 542, 748, 795, 1415, 1687
BspMAI CTGCAG 3 cut(s) 469, 1162, 1474
BspMI ACCTGC 3 cut(s) 64, 307, 1165
BspPI GGATC 3 cut(s) 661, 1867, 2105
BspT107I GGYRCC 1 cut(s) 793
BspTNI GGTCTC 3 cut(s) 1512, 1925, 2264
BsrDI GCAATG 4 cut(s) 480, 1629, 2155, 2222
BsrI ACTGG 9 cut(s) 117, 526, 613, 905, 1797, 1852, 2228, 2420, 2507
BssECI CCNNGG 8 cut(s) 363, 364, 449, 654, 1812, 1959, 2277, 2323
BssMI GATC 8 cut(s) 666, 676, 866, 994, 1430, 1859, 1895, 2097
BssNI GRCGYC 1 cut(s) 794
BssSI CACGAG 2 cut(s) 391, 621
BssT1I CCWWGG 5 cut(s) 449, 1812, 1959, 2277, 2323
Bst2BI CACGAG 2 cut(s) 391, 621
Bst2UI CCWGG 6 cut(s) 365, 552, 564, 635, 655, 2249
Bst4CI ACNGT 2 cut(s) 743, 2108
Bst6I CTCTTC 2 cut(s) 1563, 2457
BstACI GRCGYC 1 cut(s) 794
BstAPI GCANNNNNTGC 1 cut(s) 464
BstC8I GCNNGC 7 cut(s) 33, 378, 628, 798, 802, 1022, 1424
BstDEI CTNAG 8 cut(s) 69, 266, 438, 882, 1100, 1187, 1465, 1498
BstDSI CCRYGG 2 cut(s) 1959, 2323
BstEII GGTNACC 1 cut(s) 2055
BstF5I GGATG 8 cut(s) 25, 442, 460, 847, 1267, 1370, 1955, 2166
BstFNI CGCG 2 cut(s) 705, 1422
BstH2I RGCGCY 1 cut(s) 797
BstHHI GCGC 9 cut(s) 707, 781, 796, 822, 1038, 1114, 1422, 2080, 2287
BstKTI GATC 8 cut(s) 669, 679, 869, 997, 1433, 1862, 1898, 2100
BstMAI GTCTC 7 cut(s) 327, 1096, 1293, 1512, 1925, 2264, 2462
BstMBI GATC 8 cut(s) 666, 676, 866, 994, 1430, 1859, 1895, 2097
BstNI CCWGG 6 cut(s) 365, 552, 564, 635, 655, 2249
BstNSI RCATGY 6 cut(s) 35, 769, 1942, 1952, 2266, 2485
BstPAI GACNNNNGTC 2 cut(s) 475, 2422
BstPI GGTNACC 1 cut(s) 2055
BstSCI CCNGG 6 cut(s) 363, 550, 562, 633, 653, 2247
BstSFI CTRYAG 6 cut(s) 465, 708, 732, 1158, 1470, 1908
BstSLI GKGCMC 2 cut(s) 155, 1395
BstUI CGCG 2 cut(s) 705, 1422
BstV2I GAAGAC 3 cut(s) 1156, 2042, 2375
BstX2I RGATCY 6 cut(s) 666, 676, 866, 1859, 1895, 2097
BstXI CCANNNNNNTGG 1 cut(s) 551
BstYI RGATCY 6 cut(s) 666, 676, 866, 1859, 1895, 2097
BsuI GTATCC 1 cut(s) 938
BsuRI GGCC 5 cut(s) 420, 633, 1605, 1843, 1964
BtgI CCRYGG 2 cut(s) 1959, 2323
BtsCI GGATG 8 cut(s) 25, 442, 460, 847, 1267, 1370, 1955, 2166
BtsI GCAGTG 3 cut(s) 1666, 2445, 2476
BtsIMutI CAGTG 8 cut(s) 274, 411, 606, 912, 1666, 2427, 2445, 2476
BveI ACCTGC 3 cut(s) 64, 307, 1165
Cac8I GCNNGC 7 cut(s) 33, 378, 628, 798, 802, 1022, 1424
CciI TCATGA 2 cut(s) 100, 1902
CfoI GCGC 9 cut(s) 707, 781, 796, 822, 1038, 1114, 1422, 2080, 2287
Cfr13I GGNCC 8 cut(s) 541, 566, 890, 1236, 1487, 1816, 1841, 1963
Csp6I GTAC 2 cut(s) 61, 2067
CspCI CAANNNNNGTGG 2 cut(s) 2263, 2298
CviQI GTAC 2 cut(s) 61, 2067
DdeI CTNAG 8 cut(s) 69, 266, 438, 882, 1100, 1187, 1465, 1498
DinI GGCGCC 1 cut(s) 795
DpnI GATC 8 cut(s) 668, 678, 868, 996, 1432, 1861, 1897, 2099
DpnII GATC 8 cut(s) 666, 676, 866, 994, 1430, 1859, 1895, 2097
DraI TTTAAA 1 cut(s) 720
DraIII CACNNNGTG 1 cut(s) 331
EaeI YGGCCR 2 cut(s) 418, 1603
Eam1104I CTCTTC 2 cut(s) 1563, 2457
EarI CTCTTC 2 cut(s) 1563, 2457
EciI GGCGGA 1 cut(s) 435
Eco130I CCWWGG 5 cut(s) 449, 1812, 1959, 2277, 2323
Eco31I GGTCTC 3 cut(s) 1512, 1925, 2264
Eco47I GGWCC 6 cut(s) 541, 566, 890, 1236, 1487, 1816
Eco57I CTGAAG 5 cut(s) 1047, 1635, 1977, 2094, 2237
Eco88I CYCGRG 2 cut(s) 86, 1509
Eco91I GGTNACC 1 cut(s) 2055
EcoO65I GGTNACC 1 cut(s) 2055
EcoRI GAATTC 1 cut(s) 2348
EcoRII CCWGG 6 cut(s) 363, 550, 562, 633, 653, 2247
EcoT14I CCWWGG 5 cut(s) 449, 1812, 1959, 2277, 2323
EcoT22I ATGCAT 1 cut(s) 37
EgeI GGCGCC 1 cut(s) 795
EheI GGCGCC 1 cut(s) 795
ErhI CCWWGG 5 cut(s) 449, 1812, 1959, 2277, 2323
Esp3I CGTCTC 2 cut(s) 1293, 2462
FaqI GGGAC 1 cut(s) 382
FauNDI CATATG 2 cut(s) 999, 2428
FbaI TGATCA 1 cut(s) 994
FokI GGATG 8 cut(s) 32, 429, 467, 854, 1274, 1357, 1942, 2153
FspBI CTAG 3 cut(s) 644, 939, 1863
GlaI GCGC 9 cut(s) 706, 780, 795, 821, 1037, 1113, 1421, 2079, 2286
GsaI CCCAGC 2 cut(s) 1340, 1506
GsuI CTGGAG 3 cut(s) 630, 888, 2288
HaeII RGCGCY 1 cut(s) 797
HaeIII GGCC 5 cut(s) 420, 633, 1605, 1843, 1964
HapII CCGG 1 cut(s) 569
HhaI GCGC 9 cut(s) 707, 781, 796, 822, 1038, 1114, 1422, 2080, 2287
Hin1I GRCGYC 1 cut(s) 794
Hin6I GCGC 9 cut(s) 705, 779, 794, 820, 1036, 1112, 1420, 2078, 2285
HinP1I GCGC 9 cut(s) 705, 779, 794, 820, 1036, 1112, 1420, 2078, 2285
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HindIII AAGCTT 1 cut(s) 1791
HinfI GANTC 9 cut(s) 124, 157, 401, 572, 582, 1049, 1332, 2162, 2335
HpaII CCGG 1 cut(s) 569
HphI GGTGA 3 cut(s) 421, 2049, 2227
Hpy166II GTNNAC 4 cut(s) 63, 256, 2039, 2500
Hpy188I TCNGA 9 cut(s) 873, 885, 923, 1435, 2001, 2194, 2217, 2347, 2516
Hpy8I GTNNAC 4 cut(s) 63, 256, 2039, 2500
Hpy99I CGWCG 1 cut(s) 688
HpyCH4III ACNGT 2 cut(s) 743, 2108
HpyCH4IV ACGT 2 cut(s) 827, 1093
HpyF3I CTNAG 8 cut(s) 69, 266, 438, 882, 1100, 1187, 1465, 1498
HpySE526I ACGT 2 cut(s) 827, 1093
Hsp92I GRCGYC 1 cut(s) 794
HspAI GCGC 9 cut(s) 705, 779, 794, 820, 1036, 1112, 1420, 2078, 2285
KasI GGCGCC 1 cut(s) 793
Kpn2I TCCGGA 1 cut(s) 568
Ksp22I TGATCA 1 cut(s) 994
Kzo9I GATC 8 cut(s) 666, 676, 866, 994, 1430, 1859, 1895, 2097
LmnI GCTCC 2 cut(s) 1714, 2131
MaeI CTAG 3 cut(s) 644, 939, 1863
MaeII ACGT 2 cut(s) 827, 1093
MaeIII GTNAC 7 cut(s) 344, 409, 1135, 1267, 1380, 2055, 2108
MalI GATC 8 cut(s) 668, 678, 868, 996, 1432, 1861, 1897, 2099
MboI GATC 8 cut(s) 666, 676, 866, 994, 1430, 1859, 1895, 2097
MfeI CAATTG 1 cut(s) 1713
MflI RGATCY 6 cut(s) 666, 676, 866, 1859, 1895, 2097
MhlI GDGCHC 4 cut(s) 155, 1395, 1428, 2345
MlsI TGGCCA 2 cut(s) 420, 1605
MluCI AATT 8 cut(s) 1348, 1589, 1713, 1836, 1919, 2029, 2199, 2348
MluNI TGGCCA 2 cut(s) 420, 1605
Mly113I GGCGCC 1 cut(s) 794
MlyI GAGTC 1 cut(s) 2329
MmeI TCCRAC 3 cut(s) 189, 917, 2075
Mox20I TGGCCA 2 cut(s) 420, 1605
Mph1103I ATGCAT 1 cut(s) 37
MroI TCCGGA 1 cut(s) 568
MroXI GAANNNNTTC 1 cut(s) 1641
MscI TGGCCA 2 cut(s) 420, 1605
MseI TTAA 8 cut(s) 186, 719, 1175, 1200, 1212, 1310, 1479, 1587
MslI CAYNNNNRTG 2 cut(s) 611, 2193
Msp20I TGGCCA 2 cut(s) 420, 1605
MspA1I CMGCKG 4 cut(s) 1099, 1340, 1469, 1475
MspI CCGG 1 cut(s) 569
MspR9I CCNGG 6 cut(s) 365, 552, 564, 635, 655, 2249
MunI CAATTG 1 cut(s) 1713
Mva1269I GAATGC 1 cut(s) 1681
MvaI CCWGG 6 cut(s) 365, 552, 564, 635, 655, 2249
MvnI CGCG 2 cut(s) 705, 1422
NarI GGCGCC 1 cut(s) 794
NcoI CCATGG 2 cut(s) 1959, 2323
NdeI CATATG 2 cut(s) 999, 2428
NdeII GATC 8 cut(s) 666, 676, 866, 994, 1430, 1859, 1895, 2097
NlaIV GGNNCC 6 cut(s) 197, 542, 748, 795, 1415, 1687
NmuCI GTSAC 3 cut(s) 409, 1135, 2055
NsiI ATGCAT 1 cut(s) 37
NspI RCATGY 6 cut(s) 35, 769, 1942, 1952, 2266, 2485
OliI CACNNNNGTG 1 cut(s) 611
PaeI GCATGC 1 cut(s) 35
PaeR7I CTCGAG 2 cut(s) 86, 1509
PagI TCATGA 2 cut(s) 100, 1902
PaqCI CACCTGC 1 cut(s) 307
PasI CCCWGGG 1 cut(s) 364
PctI GAATGC 1 cut(s) 1681
PdmI GAANNNNTTC 1 cut(s) 1641
PfeI GAWTC 8 cut(s) 124, 157, 401, 572, 582, 1049, 1332, 2162
PleI GAGTC 1 cut(s) 2329
PluTI GGCGCC 1 cut(s) 797
PpsI GAGTC 1 cut(s) 2329
PshAI GACNNNNGTC 2 cut(s) 475, 2422
Psp6I CCWGG 6 cut(s) 363, 550, 562, 633, 653, 2247
PspEI GGTNACC 1 cut(s) 2055
PspFI CCCAGC 2 cut(s) 1336, 1502
PspGI CCWGG 6 cut(s) 363, 550, 562, 633, 653, 2247
PspN4I GGNNCC 6 cut(s) 197, 542, 748, 795, 1415, 1687
PspPI GGNCC 8 cut(s) 541, 566, 890, 1236, 1487, 1816, 1841, 1963
PstI CTGCAG 3 cut(s) 469, 1162, 1474
PsuI RGATCY 6 cut(s) 666, 676, 866, 1859, 1895, 2097
PvuII CAGCTG 4 cut(s) 1099, 1340, 1469, 1475
RsaI GTAC 2 cut(s) 62, 2068
RsaNI GTAC 2 cut(s) 61, 2067
RseI CAYNNNNRTG 2 cut(s) 611, 2193
SaqAI TTAA 8 cut(s) 186, 719, 1175, 1200, 1212, 1310, 1479, 1587
Sau3AI GATC 8 cut(s) 666, 676, 866, 994, 1430, 1859, 1895, 2097
Sau96I GGNCC 8 cut(s) 541, 566, 890, 1236, 1487, 1816, 1841, 1963
SbfI CCTGCAGG 1 cut(s) 469
SchI GAGTC 1 cut(s) 2329
ScrFI CCNGG 6 cut(s) 365, 552, 564, 635, 655, 2249
SdaI CCTGCAGG 1 cut(s) 469
SduI GDGCHC 4 cut(s) 155, 1395, 1428, 2345
SfcI CTRYAG 6 cut(s) 465, 708, 732, 1158, 1470, 1908
SfoI GGCGCC 1 cut(s) 795
Sfr274I CTCGAG 2 cut(s) 86, 1509
SinI GGWCC 6 cut(s) 541, 566, 890, 1236, 1487, 1816
SlaI CTCGAG 2 cut(s) 86, 1509
SmiMI CAYNNNNRTG 2 cut(s) 611, 2193
SmlI CTYRAG 3 cut(s) 86, 1509, 1559
SmoI CTYRAG 3 cut(s) 86, 1509, 1559
SphI GCATGC 1 cut(s) 35
Sse8387I CCTGCAGG 1 cut(s) 469
Sse9I AATT 8 cut(s) 1348, 1589, 1713, 1836, 1919, 2029, 2199, 2348
SsiI CCGC 6 cut(s) 130, 446, 630, 662, 703, 1085
SspDI GGCGCC 1 cut(s) 793
SspI AATATT 2 cut(s) 1453, 1871
SspMI CTAG 3 cut(s) 644, 939, 1863
StyD4I CCNGG 6 cut(s) 363, 550, 562, 633, 653, 2247
StyI CCWWGG 5 cut(s) 449, 1812, 1959, 2277, 2323
TaaI ACNGT 2 cut(s) 743, 2108
TaiI ACGT 2 cut(s) 830, 1096
TaqI TCGA 6 cut(s) 87, 122, 171, 977, 1510, 2405
TaqII GACCGA 2 cut(s) 1475, 2043
TasI AATT 8 cut(s) 1348, 1589, 1713, 1836, 1919, 2029, 2199, 2348
TauI GCSGC 4 cut(s) 132, 633, 705, 1088
TfiI GAWTC 8 cut(s) 124, 157, 401, 572, 582, 1049, 1332, 2162
Tru1I TTAA 8 cut(s) 186, 719, 1175, 1200, 1212, 1310, 1479, 1587
Tru9I TTAA 8 cut(s) 186, 719, 1175, 1200, 1212, 1310, 1479, 1587
TscAI CASTG 8 cut(s) 274, 411, 613, 912, 1666, 2427, 2445, 2483
TseFI GTSAC 3 cut(s) 409, 1135, 2055
Tsp45I GTSAC 3 cut(s) 409, 1135, 2055
TspDTI ATGAA 6 cut(s) 290, 351, 572, 1062, 1581, 2166
TspGWI ACGGA 1 cut(s) 341
TspRI CASTG 8 cut(s) 274, 411, 613, 912, 1666, 2427, 2445, 2483
VpaK11BI GGWCC 6 cut(s) 541, 566, 890, 1236, 1487, 1816
XapI RAATTY 3 cut(s) 1919, 2029, 2348
XbaI TCTAGA 1 cut(s) 643
XceI RCATGY 6 cut(s) 35, 769, 1942, 1952, 2266, 2485
XhoI CTCGAG 2 cut(s) 86, 1509
XmnI GAANNNNTTC 1 cut(s) 1641
XspI CTAG 3 cut(s) 644, 939, 1863
Zsp2I ATGCAT 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.