MD09G1211100.v1.1
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0009610
Physical Location & Seq
Reverse (-)
20573909 .. 20579137
5229 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1211100.v1.1.491

Sequence Viewer

Length: 2112 bp
ATGAACGGAGTTGGGAGGCAAGGGCAGAGATCTGGTGCGGCGGGGGTGCACCACCAGCGGCAATACTCTGATAACTTCTTGGAGACTTCGTCCAATGGGAGGTGGCTTCAGTCAGCTGGTCTTCAACACCTTCAGACTTCTTCCAACTCATCATCTGGTCCTCCTCCTCAGGACTATGGTTTCTATGGTGGAGGTGGAGGAGGACCGGCTTCTAGAGTGTACCGGAACGCACAGAGAGGGTACAATGAGTTTTATGCGGAGGCTCCATCCACACCTCCCTTCAATTCCCGGAGGAAGAGCGACGAGGAGTCACCCAATGAGTTCAGTCCTGGACTCTTGGATCTGCATTCCTTTGATACTGAGCTCATACCTGATATGCCAACCTCTGGGTTGCATGATGGAGCTTCTCTATATTATCCTTCAAGGGGAAGAAGCTTTGATGATTCTGAACCCTACATTTCTAATAACAAACAAACAGAAAGGGCTCGGGTACCAGAAAACAACCTCCTAAAAAGTTTTGCCGCAGACAAAGAGAAAGCTAGTTCTGTAGCAAAGATCAAAGTTGTGGTGCGCAAGAGACCACTTAATAAAAAGGAGTTGGCAAAGAATGAGGAAGATATCATAGATACACTTTCAAACTCCGTGACAGTTCATGAGACTAAGCTCAAGGTTGACCTAACAGAATACATGGAAAAACATGAATTTGTGTTTGATGCAGTGTTGAACGAGGAAGTCTCCAATGATGAAGTATATCATGAGACTGTGGAGCCCATAGTTCCAATAATTTTCCAACGCACGAAGGCAACTTGTTTTGCATATGGACAAACTGGGAGTGGAAAAACATATACCATGAAACCATTGCCTCTTAAAGCATCACGAGACATCTTGAGGTTGATGCACCATACATACCGTAGCCAGGGATTTCAGTTGTTTGTGAGCTTCTTCGAAATATATGGGGGAAAACTTTTTGATCTCCTCAATGATCGAAAAAAGCTTTGCATGAGAGAGGATGGTAAACAACAGGTTTGTATTGTGGGTTTGCAAGAATACAGAGTGTCAGATGTGGAGACCATTAAGGAGCTCATTGAGAGAGGAAGTGCCACAAGAAGTACAGGTACAACTGGTGCAAATGAAGAATCCTCTCGTTCACATGCAATACTTCAGCTTGCTATTAAGAGGTCAGTTGATGGCAATGAAAAGAAACCTCCCCGTCTGGTCGGCAAGCTGTCCTTTATAGATCTTGCTGGAAGTGAACGTGGTGCAGATACTACAGATAATGATAAACAGACAAGAATGGAAGGTGCTGAGATTAATAAGAGCTTACTTGCATTGAAGGAATGCATTAGAGCTCTTGACAATGACCAGGGTCATATTCCTTTCCGAGGAAGTAAATTAACTGAAGTTCTTAGGGACTCATTTGTTGGTGACTCCCGTACTGTGATGATATCATGCATATCACCAAGCTCAGGATCCTGTGAACATACTCTCAATACTTTAAGATATGCTGACAGGGTCAAAAGCCTTTCAAAAGGGAACAACCCCAAGAAGGATATGTTACCTTCAACGCTAAACTTCAAGGAATCAACAAATATACCCCTATCTTCAGCTTTGCCCACTGCTTCGGCCTATGAAGTTGACACAAATGATACATGGACTGAACAAATTGAAACGGAGGAATTTGATGCATCAGAAGAGTCTTGTTACGAGGCCAAAACATCATGGAAGAGAAATGGGAAGCTAGAGCAGTACAATGTATCAGCTACAGAGGACAAAGTACGTAAGTCCAATGGTCAGACAAAATCAGAACTGCCAAAGTTTCATTCAAGGAACTCAAACTCAGATGACGATTTGAATGCCCTGCTACAGGAGGAGGAAGATCTTGTAAGTGCTCACCGGAAACAAGTTGAGGACACTATGAATATTGTTAAAGAGGAGATGAATCTGTTGGTAGACGCAGATCAACCCGGGAATCAGCTGGACAACTATGTTTCTCGTTTGAATGCCATTCTATCTCAGAAGGCTGCAGGCATCCTGCAACTACAAAATCGGTTAGCTCATTTCCAGAAGCGGTTAAAGGAACACAATGTCATAGTGTCCTCTTCGGGGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000226 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000819 GO:0002376 GO:0002478 GO:0002495 GO:0002504 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005829 GO:0005856 GO:0005871 GO:0005874 GO:0005875 GO:0005886 GO:0006810 GO:0006890 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007019 GO:0007049 GO:0007059 GO:0007080 GO:0007163 GO:0008017 GO:0008092 GO:0008150 GO:0008283 GO:0008608 GO:0009987 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019237 GO:0019882 GO:0019884 GO:0019886 GO:0022402 GO:0022411 GO:0030951 GO:0030952 GO:0031109 GO:0032984 GO:0032991 GO:0035371 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043565 GO:0043624 GO:0043933 GO:0044422 GO:0044424 GO:0044427 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0048002 GO:0048193 GO:0048285 GO:0050000 GO:0050789 GO:0050794 GO:0051010 GO:0051179 GO:0051234 GO:0051261 GO:0051276 GO:0051303 GO:0051310 GO:0051315 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0051983 GO:0065007 GO:0071840 GO:0071944 GO:0097159 GO:0097435 GO:0098687 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140014 GO:1901363 GO:1903047 GO:1903338 GO:1990752 GO:1990837
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kinesin

Protein Analysis

704

Amino Acids

78.57

Weight (kDa)

6.02

Isoelectric Point (pI)

49.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Microtub_bd PF16796 185 - 326 4.2e-14 Microtubule binding
Kinesin PF00225 191 - 507 4.2e-100 Kinesin motor domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 572
Acc65I GGTACC 1 cut(s) 490
AccB1I GGYRCC 1 cut(s) 490
AccB7I CCANNNNNTGG 1 cut(s) 386
AccI GTMKAC 1 cut(s) 1950
AciI CCGC 6 cut(s) 38, 41, 58, 257, 522, 2068
AclWI GGATC 3 cut(s) 348, 1464, 1477
AcsI RAATTY 2 cut(s) 701, 1676
AcuI CTGAAG 5 cut(s) 92, 116, 1145, 1419, 1587
AfaI GTAC 9 cut(s) 221, 242, 492, 1111, 1117, 1435, 1748, 1776, 2108
AfiI CCNNNNNNNGG 9 cut(s) 99, 386, 425, 916, 1382, 1466, 1546, 1864, 2103
AhdI GACNNNNNGTC 1 cut(s) 307
AjnI CCWGG 3 cut(s) 328, 915, 1362
AjuI GAANNNNNNNTTGG 2 cut(s) 1804, 1836
Alw21I GWGCWC 5 cut(s) 51, 366, 1083, 1351, 1891
Alw26I GTCTC 7 cut(s) 77, 571, 650, 739, 752, 873, 1061
Alw44I GTGCAC 1 cut(s) 47
AlwI GGATC 3 cut(s) 348, 1464, 1477
Ama87I CYCGRG 2 cut(s) 486, 1964
AoxI GGCC 2 cut(s) 1623, 1707
ApaLI GTGCAC 1 cut(s) 47
ApeKI GCWGC 1 cut(s) 2021
ApoI RAATTY 2 cut(s) 701, 1676
AseI ATTAAT 1 cut(s) 1311
Asp718I GGTACC 1 cut(s) 490
AspLEI GCGC 1 cut(s) 573
AspS9I GGNCC 2 cut(s) 158, 203
AsuC2I CCSGG 3 cut(s) 289, 1965, 1966
AsuHPI GGTGA 4 cut(s) 303, 1436, 1449, 1883
AsuII TTCGAA 1 cut(s) 945
AvaI CYCGRG 2 cut(s) 486, 1964
AvaII GGWCC 2 cut(s) 158, 203
AxyI CCTNAGG 1 cut(s) 168
BaeGI GKGCMC 1 cut(s) 51
BamHI GGATCC 1 cut(s) 1469
BanI GGYRCC 1 cut(s) 490
BanII GRGCYC 5 cut(s) 366, 487, 771, 1083, 1351
BarI GAAGNNNNNNTAC 2 cut(s) 1099, 1131
BauI CACGAG 1 cut(s) 876
BbsI GAAGAC 1 cut(s) 113
Bbv12I GWGCWC 5 cut(s) 51, 366, 1083, 1351, 1891
BbvI GCAGC 1 cut(s) 2008
BccI CCATC 4 cut(s) 274, 392, 1004, 1181
BcgI CGANNNNNNTGC 2 cut(s) 1835, 1869
BciT130I CCWGG 3 cut(s) 330, 917, 1364
BcnI CCSGG 3 cut(s) 289, 1965, 1966
BcoDI GTCTC 7 cut(s) 77, 571, 650, 739, 752, 873, 1061
BfaI CTAG 3 cut(s) 213, 540, 1739
BfmI CTRYAG 5 cut(s) 546, 1269, 1761, 1862, 2022
BglII AGATCT 3 cut(s) 29, 1237, 1876
BisI GCNGC 4 cut(s) 39, 59, 522, 2022
BlsI GCNGC 4 cut(s) 40, 60, 523, 2023
Bme1390I CCNGG 6 cut(s) 289, 330, 917, 1364, 1965, 1966
Bme18I GGWCC 2 cut(s) 158, 203
BmeRI GACNNNNNGTC 1 cut(s) 307
BmeT110I CYCGRG 2 cut(s) 486, 1964
BmgT120I GGNCC 2 cut(s) 158, 203
BmiI GGNNCC 4 cut(s) 264, 492, 768, 1471
BmrFI CCNGG 6 cut(s) 289, 330, 917, 1364, 1965, 1966
BmrI ACTGGG 1 cut(s) 837
BmsI GCATC 6 cut(s) 703, 881, 885, 1672, 1694, 2037
BmuI ACTGGG 1 cut(s) 837
BoxI GACNNNNGTC 1 cut(s) 1365
BpiI GAAGAC 1 cut(s) 113
BplI GAGNNNNNCTC 2 cut(s) 719, 751
Bpu10I CCTNAGC 1 cut(s) 1465
Bpu14I TTCGAA 1 cut(s) 945
BpuEI CTTGAG 2 cut(s) 650, 907
BpuMI CCSGG 3 cut(s) 289, 1965, 1966
BsaAI YACGTR 1 cut(s) 1778
BsaI GGTCTC 2 cut(s) 571, 1061
BsaJI CCNNGG 4 cut(s) 916, 1363, 1381, 1964
BsaWI WCCGGW 2 cut(s) 222, 1893
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 9 cut(s) 99, 386, 425, 916, 1382, 1466, 1546, 1864, 2103
Bse118I RCCGGY 1 cut(s) 205
Bse1I ACTGG 2 cut(s) 832, 1126
Bse21I CCTNAGG 1 cut(s) 168
Bse3DI GCAATG 2 cut(s) 857, 1198
BseBI CCWGG 3 cut(s) 330, 917, 1364
BseDI CCNNGG 4 cut(s) 916, 1363, 1381, 1964
BseGI GGATG 3 cut(s) 266, 1015, 2028
BseLI CCNNNNNNNGG 9 cut(s) 99, 386, 425, 916, 1382, 1466, 1546, 1864, 2103
BseMI GCAATG 2 cut(s) 857, 1198
BseMII CTCAG 6 cut(s) 182, 351, 1296, 1479, 1851, 2027
BseNI ACTGG 2 cut(s) 832, 1126
BseRI GAGGAG 7 cut(s) 153, 156, 213, 320, 965, 1883, 1946
BseSI GKGCMC 1 cut(s) 51
BseXI GCAGC 1 cut(s) 2008
BsgI GTGCAG 1 cut(s) 1281
BshFI GGCC 2 cut(s) 1625, 1709
BshNI GGYRCC 1 cut(s) 490
BsiHKAI GWGCWC 5 cut(s) 51, 366, 1083, 1351, 1891
BsiHKCI CYCGRG 2 cut(s) 486, 1964
BsiSI CCGG 5 cut(s) 206, 223, 289, 1894, 1965
BslFI GGGAC 1 cut(s) 1424
BslI CCNNNNNNNGG 9 cut(s) 99, 386, 425, 916, 1382, 1466, 1546, 1864, 2103
BsmAI GTCTC 7 cut(s) 77, 571, 650, 739, 752, 873, 1061
BsmFI GGGAC 1 cut(s) 1424
BsmI GAATGC 4 cut(s) 346, 1343, 1858, 2005
BsnI GGCC 2 cut(s) 1625, 1709
Bso31I GGTCTC 2 cut(s) 571, 1061
BsoBI CYCGRG 2 cut(s) 486, 1964
Bsp119I TTCGAA 1 cut(s) 945
Bsp1286I GDGCHC 7 cut(s) 51, 366, 487, 771, 1083, 1351, 1891
Bsp143I GATC 9 cut(s) 29, 340, 555, 970, 982, 1237, 1469, 1876, 1957
BspACI CCGC 6 cut(s) 38, 41, 58, 257, 522, 2068
BspANI GGCC 2 cut(s) 1625, 1709
BspCNI CTCAG 6 cut(s) 181, 352, 1297, 1478, 1850, 2026
BspHI TCATGA 2 cut(s) 652, 754
BspLI GGNNCC 4 cut(s) 264, 492, 768, 1471
BspMAI CTGCAG 1 cut(s) 2026
BspPI GGATC 3 cut(s) 348, 1464, 1477
BspQI GCTCTTC 1 cut(s) 290
BspT104I TTCGAA 1 cut(s) 945
BspT107I GGYRCC 1 cut(s) 490
BspTNI GGTCTC 2 cut(s) 571, 1061
BsrDI GCAATG 2 cut(s) 857, 1198
BsrFI RCCGGY 1 cut(s) 205
BsrI ACTGG 2 cut(s) 832, 1126
BssAI RCCGGY 1 cut(s) 205
BssECI CCNNGG 4 cut(s) 916, 1363, 1381, 1964
BssMI GATC 9 cut(s) 29, 340, 555, 970, 982, 1237, 1469, 1876, 1957
BssSI CACGAG 1 cut(s) 876
Bst2BI CACGAG 1 cut(s) 876
Bst2UI CCWGG 3 cut(s) 330, 917, 1364
Bst4CI ACNGT 4 cut(s) 649, 763, 911, 1438
Bst6I CTCTTC 4 cut(s) 290, 1686, 1718, 2104
BstBAI YACGTR 1 cut(s) 1778
BstBI TTCGAA 1 cut(s) 945
BstC8I GCNNGC 3 cut(s) 1167, 1223, 2026
BstDEI CTNAG 8 cut(s) 168, 360, 660, 1305, 1406, 1465, 1837, 2013
BstENI CCTNNNNNAGG 2 cut(s) 1380, 1862
BstF5I GGATG 3 cut(s) 266, 1015, 2028
BstHHI GCGC 1 cut(s) 573
BstKTI GATC 9 cut(s) 32, 343, 558, 973, 985, 1240, 1472, 1879, 1960
BstMAI GTCTC 7 cut(s) 77, 571, 650, 739, 752, 873, 1061
BstMBI GATC 9 cut(s) 29, 340, 555, 970, 982, 1237, 1469, 1876, 1957
BstMWI GCNNNNNNNGC 1 cut(s) 55
BstNI CCWGG 3 cut(s) 330, 917, 1364
BstNSI RCATGY 1 cut(s) 1154
BstPAI GACNNNNGTC 1 cut(s) 1365
BstSCI CCNGG 6 cut(s) 287, 328, 915, 1362, 1963, 1964
BstSFI CTRYAG 5 cut(s) 546, 1269, 1761, 1862, 2022
BstSLI GKGCMC 1 cut(s) 51
BstSNI TACGTA 1 cut(s) 1778
BstV1I GCAGC 1 cut(s) 2008
BstV2I GAAGAC 1 cut(s) 113
BstX2I RGATCY 5 cut(s) 29, 340, 1237, 1469, 1876
BstYI RGATCY 5 cut(s) 29, 340, 1237, 1469, 1876
Bsu36I CCTNAGG 1 cut(s) 168
BsuRI GGCC 2 cut(s) 1625, 1709
BtsCI GGATG 3 cut(s) 266, 1015, 2028
BtsI GCAGTG 2 cut(s) 723, 1614
BtsIMutI CAGTG 2 cut(s) 723, 1614
Cac8I GCNNGC 3 cut(s) 1167, 1223, 2026
CciI TCATGA 2 cut(s) 652, 754
CfoI GCGC 1 cut(s) 573
Cfr10I RCCGGY 1 cut(s) 205
Cfr13I GGNCC 2 cut(s) 158, 203
Cfr9I CCCGGG 1 cut(s) 1964
CseI GACGC 1 cut(s) 1961
Csp6I GTAC 9 cut(s) 220, 241, 491, 1110, 1116, 1434, 1747, 1775, 2107
CviQI GTAC 9 cut(s) 220, 241, 491, 1110, 1116, 1434, 1747, 1775, 2107
DdeI CTNAG 8 cut(s) 168, 360, 660, 1305, 1406, 1465, 1837, 2013
DpnI GATC 9 cut(s) 31, 342, 557, 972, 984, 1239, 1471, 1878, 1959
DpnII GATC 9 cut(s) 29, 340, 555, 970, 982, 1237, 1469, 1876, 1957
DriI GACNNNNNGTC 1 cut(s) 307
Eam1104I CTCTTC 4 cut(s) 290, 1686, 1718, 2104
Eam1105I GACNNNNNGTC 1 cut(s) 307
EarI CTCTTC 4 cut(s) 290, 1686, 1718, 2104
Ecl136II GAGCTC 3 cut(s) 364, 1081, 1349
Eco105I TACGTA 1 cut(s) 1778
Eco24I GRGCYC 5 cut(s) 366, 487, 771, 1083, 1351
Eco31I GGTCTC 2 cut(s) 571, 1061
Eco32I GATATC 2 cut(s) 619, 1446
Eco47I GGWCC 2 cut(s) 158, 203
Eco53kI GAGCTC 3 cut(s) 364, 1081, 1349
Eco57I CTGAAG 5 cut(s) 92, 116, 1145, 1419, 1587
Eco81I CCTNAGG 1 cut(s) 168
Eco88I CYCGRG 2 cut(s) 486, 1964
EcoICRI GAGCTC 3 cut(s) 364, 1081, 1349
EcoNI CCTNNNNNAGG 2 cut(s) 1380, 1862
EcoRII CCWGG 3 cut(s) 328, 915, 1362
EcoRV GATATC 2 cut(s) 619, 1446
EcoT22I ATGCAT 3 cut(s) 1343, 1454, 1687
EcoT38I GRGCYC 5 cut(s) 366, 487, 771, 1083, 1351
FalI AAGNNNNNCTT 2 cut(s) 1214, 1246
FaqI GGGAC 1 cut(s) 1424
FauI CCCGC 1 cut(s) 34
FauNDI CATATG 1 cut(s) 817
FblI GTMKAC 1 cut(s) 1950
Fnu4HI GCNGC 4 cut(s) 39, 59, 522, 2022
FokI GGATG 3 cut(s) 253, 1022, 2015
FriOI GRGCYC 5 cut(s) 366, 487, 771, 1083, 1351
Fsp4HI GCNGC 4 cut(s) 39, 59, 522, 2022
FspBI CTAG 3 cut(s) 213, 540, 1739
FspI TGCGCA 1 cut(s) 572
GlaI GCGC 1 cut(s) 572
GluI GCNGC 4 cut(s) 39, 59, 522, 2022
HaeIII GGCC 2 cut(s) 1625, 1709
HapII CCGG 5 cut(s) 206, 223, 289, 1894, 1965
HgaI GACGC 1 cut(s) 1961
HhaI GCGC 1 cut(s) 573
Hin6I GCGC 1 cut(s) 571
HinP1I GCGC 1 cut(s) 571
HincII GTYRAC 2 cut(s) 673, 1636
HindII GTYRAC 2 cut(s) 673, 1636
HindIII AAGCTT 2 cut(s) 433, 992
HpaII CCGG 5 cut(s) 206, 223, 289, 1894, 1965
HphI GGTGA 4 cut(s) 303, 1436, 1449, 1883
Hpy166II GTNNAC 9 cut(s) 49, 220, 673, 1016, 1148, 1253, 1478, 1636, 1951
Hpy188III TCNNGA 9 cut(s) 170, 213, 653, 755, 876, 886, 1352, 1467, 2062
Hpy8I GTNNAC 9 cut(s) 49, 220, 673, 1016, 1148, 1253, 1478, 1636, 1951
Hpy99I CGWCG 1 cut(s) 305
HpyAV CCTTC 9 cut(s) 140, 289, 429, 793, 1292, 1327, 1540, 1569, 2011
HpyCH4III ACNGT 4 cut(s) 649, 763, 911, 1438
HpyCH4IV ACGT 2 cut(s) 1255, 1777
HpyF10VI GCNNNNNNNGC 1 cut(s) 55
HpyF3I CTNAG 8 cut(s) 168, 360, 660, 1305, 1406, 1465, 1837, 2013
HpySE526I ACGT 2 cut(s) 1255, 1777
HspAI GCGC 1 cut(s) 571
KpnI GGTACC 1 cut(s) 494
Kzo9I GATC 9 cut(s) 29, 340, 555, 970, 982, 1237, 1469, 1876, 1957
LguI GCTCTTC 1 cut(s) 290
LmnI GCTCC 4 cut(s) 268, 401, 766, 1078
Lsp1109I GCAGC 1 cut(s) 2008
LweI GCATC 6 cut(s) 703, 881, 885, 1672, 1694, 2037
MaeI CTAG 3 cut(s) 213, 540, 1739
MaeII ACGT 2 cut(s) 1255, 1777
MaeIII GTNAC 5 cut(s) 309, 643, 1424, 1554, 1700
MalI GATC 9 cut(s) 31, 342, 557, 972, 984, 1239, 1471, 1878, 1959
MboI GATC 9 cut(s) 29, 340, 555, 970, 982, 1237, 1469, 1876, 1957
MflI RGATCY 5 cut(s) 29, 340, 1237, 1469, 1876
MhlI GDGCHC 7 cut(s) 51, 366, 487, 771, 1083, 1351, 1891
MluCI AATT 6 cut(s) 283, 701, 783, 1391, 1662, 1676
MlyI GAGTC 5 cut(s) 317, 327, 1406, 1421, 1703
MmeI TCCRAC 2 cut(s) 168, 814
Mph1103I ATGCAT 3 cut(s) 1343, 1454, 1687
MseI TTAA 9 cut(s) 585, 867, 1074, 1173, 1311, 1394, 1496, 1926, 2072
MspA1I CMGCKG 3 cut(s) 58, 116, 1975
MspI CCGG 5 cut(s) 206, 223, 289, 1894, 1965
MspR9I CCNGG 6 cut(s) 289, 330, 917, 1364, 1965, 1966
Mva1269I GAATGC 4 cut(s) 346, 1343, 1858, 2005
MvaI CCWGG 3 cut(s) 330, 917, 1364
MwoI GCNNNNNNNGC 1 cut(s) 55
NciI CCSGG 3 cut(s) 289, 1965, 1966
NdeI CATATG 1 cut(s) 817
NdeII GATC 9 cut(s) 29, 340, 555, 970, 982, 1237, 1469, 1876, 1957
NlaIV GGNNCC 4 cut(s) 264, 492, 768, 1471
NmuCI GTSAC 3 cut(s) 309, 643, 1424
NsbI TGCGCA 1 cut(s) 572
NsiI ATGCAT 3 cut(s) 1343, 1454, 1687
NspI RCATGY 1 cut(s) 1154
NspV TTCGAA 1 cut(s) 945
PagI TCATGA 2 cut(s) 652, 754
PciSI GCTCTTC 1 cut(s) 290
PctI GAATGC 4 cut(s) 346, 1343, 1858, 2005
PfeI GAWTC 5 cut(s) 443, 1136, 1580, 1939, 1969
PflFI GACNNNGTC 2 cut(s) 88, 1511
PflMI CCANNNNNTGG 1 cut(s) 386
PfoI TCCNGGA 2 cut(s) 287, 328
PkrI GCNGC 4 cut(s) 40, 60, 523, 2023
PleI GAGTC 5 cut(s) 316, 327, 1406, 1421, 1702
PpsI GAGTC 5 cut(s) 316, 327, 1406, 1421, 1702
Ppu21I YACGTR 1 cut(s) 1778
PshAI GACNNNNGTC 1 cut(s) 1365
PshBI ATTAAT 1 cut(s) 1311
Psp124BI GAGCTC 3 cut(s) 366, 1083, 1351
Psp6I CCWGG 3 cut(s) 328, 915, 1362
PspGI CCWGG 3 cut(s) 328, 915, 1362
PspN4I GGNNCC 4 cut(s) 264, 492, 768, 1471
PspPI GGNCC 2 cut(s) 158, 203
PstI CTGCAG 1 cut(s) 2026
PsuI RGATCY 5 cut(s) 29, 340, 1237, 1469, 1876
PsyI GACNNNGTC 2 cut(s) 88, 1511
PvuII CAGCTG 2 cut(s) 116, 1975
RsaI GTAC 9 cut(s) 221, 242, 492, 1111, 1117, 1435, 1748, 1776, 2108
RsaNI GTAC 9 cut(s) 220, 241, 491, 1110, 1116, 1434, 1747, 1775, 2107
SacI GAGCTC 3 cut(s) 366, 1083, 1351
SapI GCTCTTC 1 cut(s) 290
SaqAI TTAA 9 cut(s) 585, 867, 1074, 1173, 1311, 1394, 1496, 1926, 2072
SatI GCNGC 4 cut(s) 39, 59, 522, 2022
Sau3AI GATC 9 cut(s) 29, 340, 555, 970, 982, 1237, 1469, 1876, 1957
Sau96I GGNCC 2 cut(s) 158, 203
SchI GAGTC 5 cut(s) 317, 327, 1406, 1421, 1703
ScrFI CCNGG 6 cut(s) 289, 330, 917, 1364, 1965, 1966
SduI GDGCHC 7 cut(s) 51, 366, 487, 771, 1083, 1351, 1891
SfaNI GCATC 6 cut(s) 703, 881, 885, 1672, 1694, 2037
SfcI CTRYAG 5 cut(s) 546, 1269, 1761, 1862, 2022
SfuI TTCGAA 1 cut(s) 945
SinI GGWCC 2 cut(s) 158, 203
SmaI CCCGGG 1 cut(s) 1966
SmlI CTYRAG 2 cut(s) 665, 886
SmoI CTYRAG 2 cut(s) 665, 886
SnaBI TACGTA 1 cut(s) 1778
Sse9I AATT 6 cut(s) 283, 701, 783, 1391, 1662, 1676
SsiI CCGC 6 cut(s) 38, 41, 58, 257, 522, 2068
SspI AATATT 1 cut(s) 1921
SspMI CTAG 3 cut(s) 213, 540, 1739
SstI GAGCTC 3 cut(s) 366, 1083, 1351
StyD4I CCNGG 6 cut(s) 287, 328, 915, 1362, 1963, 1964
TaaI ACNGT 4 cut(s) 649, 763, 911, 1438
TaiI ACGT 2 cut(s) 1258, 1780
TaqI TCGA 2 cut(s) 945, 985
TasI AATT 6 cut(s) 283, 701, 783, 1391, 1662, 1676
TatI WGTACW 2 cut(s) 1109, 1746
TauI GCSGC 3 cut(s) 41, 61, 524
TfiI GAWTC 5 cut(s) 443, 1136, 1580, 1939, 1969
Tru1I TTAA 9 cut(s) 585, 867, 1074, 1173, 1311, 1394, 1496, 1926, 2072
Tru9I TTAA 9 cut(s) 585, 867, 1074, 1173, 1311, 1394, 1496, 1926, 2072
TscAI CASTG 2 cut(s) 723, 1621
TseFI GTSAC 3 cut(s) 309, 643, 1424
TseI GCWGC 1 cut(s) 2021
Tsp45I GTSAC 3 cut(s) 309, 643, 1424
TspGWI ACGGA 3 cut(s) 21, 631, 1685
TspMI CCCGGG 1 cut(s) 1964
TspRI CASTG 2 cut(s) 723, 1621
Tth111I GACNNNGTC 2 cut(s) 88, 1511
Van91I CCANNNNNTGG 1 cut(s) 386
VneI GTGCAC 1 cut(s) 47
VpaK11BI GGWCC 2 cut(s) 158, 203
VspI ATTAAT 1 cut(s) 1311
XagI CCTNNNNNAGG 2 cut(s) 1380, 1862
XapI RAATTY 2 cut(s) 701, 1676
XbaI TCTAGA 1 cut(s) 212
XceI RCATGY 1 cut(s) 1154
XmaI CCCGGG 1 cut(s) 1964
XmiI GTMKAC 1 cut(s) 1950
XspI CTAG 3 cut(s) 213, 540, 1739
Zsp2I ATGCAT 3 cut(s) 1343, 1454, 1687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.