MD17G1193800.v1.1
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0009610
Physical Location & Seq
Reverse (-)
23253051 .. 23258660
5610 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1193800.v1.1.491

Sequence Viewer

Length: 2109 bp
ATGAACGGAGTTGGGAGGCAAGGGCAAAGATCTGGTGTGGCGGGGGTGCACCACCAGCGGCAATACTCTGATAACTTCTTGGAGACTTCGTCCAATGGGAGGTGGCTTCAGTCTGCTGGTCTTCAACACCTTCAGTCTTCTTCCAACTCATCTGGTCCTCCTCCTCAGGACTATGGTTTCTATGGTGGAGGTGGAGGAGGACCGCCTTCTAGAGTGTACCGGAATGCGCAGAGAGGGTACAATGAGTTTTACGCGGAGGCTCCGTCGACACCTCCCTTCAATTCCCGGAGGAAGAGCGACGAGGAGTCGCCCAATGAATTCAGTCCTGGACTCTTGGATCTGCATTCCTTTGATACTGAGCTCATTCCTGATCTGCCAGTCTCTGGGTTGTACGATGGCGCTTCTCTATATTATCCTTCACGGGGAAGAAGCTTTGATGATTCTGAACCCTACATTTCAAATAACAAACAAATAGAAAGGGCTCGGGTACCAGAAAACAACCTCCAGAAAAGTTTTGCTGCAGACAAAGAGAAAGCTAGTTCTGTAGCAAAGATCAAAGTTGTGGTACGCAAGAGACCACTTAATAAAAAGGAGTTAGCTAAGAATGAGGAAGATATCATAGATACACTCTCAAACTCTGTCACAGTTCATGAGACAAAGCTCAAGGTTGACCTAACAGAATACATGGAAAAGCATGAATTTGTGTTTGATGCAGTGTTGAATGAGGAAGTGTCCAATGATGAAGTGTATCATGAGACTGTGGAGCCCATAGTTCCAATAATTTTCCAACGCACAAAGGCGACCTGTTTTGCATATGGACAAACTGGGAGTGGAAAAACTTATACGATGAAACCATTGCCTCTTAAGGCATCCCGAGACATCTTGAGGTTGATGCACCATACTTACCGGAGCCAGGGATTTCAGCTGTTTGTGAGCTTCTTCGAAATATATGGGGGGAAACTTTTTGATCTCCTCAGTGATCGAAAAAAGCTTTGCATGAGAGAAGATGGTAAACAACAGGTTTGTATCGTGGGTTTGCAAGAATATAGAGTGTCAGATGTGGAGACCATTAAGGAGCTCATTGAGAGAGGAAGTGCCACAAGAAGTACTGGTACAACTGGTGCAAATGAAGAATCCTCTCGTTCACATGCAATACTTCAGCTTGCTATAAAGAGGTCAGTTGATGGCAATGAAAAGAAACCTCCCCGTCTTGTCGGCAAGCTGTCCTTTATAGATCTTGCTGGAAGTGAACGGGGTGCAGATACTACAGATAATGATAAACAGACAAGAATGGAAGGGGCTGAGATTAATAAGAGCTTACTGGCATTGAAGGAATGCATTAGAGCTCTTGACAATGACCAGGGTCATATTCCTTTCCGAGGAAGTAAACTAACGGAAGTTCTTAGGGATTCATTTGTTGGTGACTCCCGTACTGTGATGATATCGTGCATATCACCAAGCTCAGGATCCTGTGAACATACTCTCAATACTTTAAGATACGCTGACAGGGTCAAAAGCCTTTCGAAAGGGAACAACCCTAAGAAGGATATGTTACCTTCAACACTAAACCTCAAGGAATCAACAAATATACCCCTACCTTCAGCTTTGCCCACTGCTTCTGCCTACGAAGTTGACACAAATGATACATGGACTGAACAAGTTGAAAAGGAGGAATTTGATGCATCAGAAGAGTCTTATTATGAGGCCAAAACATCATGGAAGAGAAATGGGAAGCTAGAGCAGTACAATGTATCAGCTACAGAGGATAAAGTACGTAAGCCCAATGGTCAGACAAAATCAGAACTGCCAAAGTTTCATTCAAGGAAGTCAAACTCAGATGACGATTTGAATGCCCTGTTACAGGAGGAGGAAGATCTTGTAAGTGCTCATCGGAAACAAGTTGAGGACACTATGAATATTGTTAAAGAGGAGATGAATCTCTTGGTAGAAGCAGATCAACCCGGGAATCAGCTGGACGATTATGTTTCTAGACTGAATGCCATTCTATCTCAGAAGGCTGCAGGCATCCTGCAATTACAAAATCGTTTAGCTCATTTCCAGAAGCGTTTAAAGGAACACAATGTCTTAGTGTCCTCTTCGGGTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000226 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000819 GO:0002376 GO:0002478 GO:0002495 GO:0002504 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005829 GO:0005856 GO:0005871 GO:0005874 GO:0005875 GO:0005886 GO:0006810 GO:0006890 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007019 GO:0007049 GO:0007059 GO:0007080 GO:0007163 GO:0008017 GO:0008092 GO:0008150 GO:0008283 GO:0008608 GO:0009987 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019237 GO:0019882 GO:0019884 GO:0019886 GO:0022402 GO:0022411 GO:0030951 GO:0030952 GO:0031109 GO:0032984 GO:0032991 GO:0035371 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043565 GO:0043624 GO:0043933 GO:0044422 GO:0044424 GO:0044427 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0048002 GO:0048193 GO:0048285 GO:0050000 GO:0050789 GO:0050794 GO:0051010 GO:0051179 GO:0051234 GO:0051261 GO:0051276 GO:0051303 GO:0051310 GO:0051315 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0051983 GO:0065007 GO:0071840 GO:0071944 GO:0097159 GO:0097435 GO:0098687 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140014 GO:1901363 GO:1903047 GO:1903338 GO:1990752 GO:1990837
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kinesin

Protein Analysis

703

Amino Acids

78.62

Weight (kDa)

6.06

Isoelectric Point (pI)

51.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Microtub_bd PF16796 184 - 325 4.2e-14 Microtubule binding
Kinesin PF00225 190 - 506 4.3e-100 Kinesin motor domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 228
Acc65I GGTACC 1 cut(s) 487
AccB1I GGYRCC 1 cut(s) 487
AccB7I CCANNNNNTGG 1 cut(s) 383
AccI GTMKAC 1 cut(s) 266
AccII CGCG 1 cut(s) 254
AciI CCGC 4 cut(s) 41, 58, 203, 254
AclWI GGATC 3 cut(s) 345, 1461, 1474
AcsI RAATTY 3 cut(s) 317, 698, 1673
AcuI CTGAAG 4 cut(s) 92, 116, 1142, 1584
AfiI CCNNNNNNNGG 8 cut(s) 99, 383, 422, 913, 1379, 1463, 1543, 1861
AflII CTTAAG 1 cut(s) 863
AgsI TTSAA 9 cut(s) 125, 280, 459, 721, 1330, 1560, 1664, 1821, 1849
AhdI GACNNNNNGTC 1 cut(s) 304
AjnI CCWGG 3 cut(s) 325, 912, 1359
AjuI GAANNNNNNNTTGG 2 cut(s) 1801, 1833
Alw21I GWGCWC 5 cut(s) 51, 363, 1080, 1348, 1888
Alw26I GTCTC 7 cut(s) 77, 385, 568, 647, 749, 870, 1058
Alw44I GTGCAC 1 cut(s) 47
AlwI GGATC 3 cut(s) 345, 1461, 1474
AlwNI CAGNNNCTG 1 cut(s) 383
Ama87I CYCGRG 3 cut(s) 483, 873, 1961
AoxI GGCC 1 cut(s) 1704
ApaLI GTGCAC 1 cut(s) 47
ApeKI GCWGC 2 cut(s) 518, 2018
ApoI RAATTY 3 cut(s) 317, 698, 1673
AseI ATTAAT 1 cut(s) 1308
Asp718I GGTACC 1 cut(s) 487
AspLEI GCGC 2 cut(s) 229, 401
AspS9I GGNCC 2 cut(s) 155, 200
AsuC2I CCSGG 3 cut(s) 286, 1962, 1963
AsuHPI GGTGA 2 cut(s) 1433, 1446
AsuII TTCGAA 2 cut(s) 942, 1523
AvaI CYCGRG 3 cut(s) 483, 873, 1961
AvaII GGWCC 2 cut(s) 155, 200
AxyI CCTNAGG 1 cut(s) 165
BaeGI GKGCMC 1 cut(s) 51
BamHI GGATCC 1 cut(s) 1466
BanI GGYRCC 1 cut(s) 487
BanII GRGCYC 5 cut(s) 363, 484, 768, 1080, 1348
BarI GAAGNNNNNNTAC 2 cut(s) 1096, 1128
BbsI GAAGAC 2 cut(s) 113, 129
Bbv12I GWGCWC 5 cut(s) 51, 363, 1080, 1348, 1888
BbvI GCAGC 2 cut(s) 505, 2005
BccI CCATC 3 cut(s) 389, 1001, 1178
BcgI CGANNNNNNTGC 2 cut(s) 1832, 1866
BciT130I CCWGG 3 cut(s) 327, 914, 1361
BcnI CCSGG 3 cut(s) 286, 1962, 1963
BcoDI GTCTC 7 cut(s) 77, 385, 568, 647, 749, 870, 1058
BfaI CTAG 4 cut(s) 210, 537, 1736, 1989
BfmI CTRYAG 5 cut(s) 519, 543, 1266, 1758, 2019
BfoI RGCGCY 1 cut(s) 402
BfrI CTTAAG 1 cut(s) 863
BglII AGATCT 3 cut(s) 29, 1234, 1873
BisI GCNGC 3 cut(s) 59, 519, 2019
BlsI GCNGC 3 cut(s) 60, 520, 2020
BmcAI AGTACT 1 cut(s) 1108
Bme1390I CCNGG 6 cut(s) 286, 327, 914, 1361, 1962, 1963
Bme18I GGWCC 2 cut(s) 155, 200
BmeRI GACNNNNNGTC 1 cut(s) 304
BmeT110I CYCGRG 3 cut(s) 483, 873, 1961
BmgT120I GGNCC 2 cut(s) 155, 200
BmiI GGNNCC 5 cut(s) 261, 489, 765, 911, 1468
BmrFI CCNGG 6 cut(s) 286, 327, 914, 1361, 1962, 1963
BmrI ACTGGG 1 cut(s) 834
BmsI GCATC 6 cut(s) 700, 878, 882, 1669, 1691, 2034
BmuI ACTGGG 1 cut(s) 834
BoxI GACNNNNGTC 1 cut(s) 1362
BpiI GAAGAC 2 cut(s) 113, 129
BpmI CTGGAG 1 cut(s) 488
Bpu10I CCTNAGC 1 cut(s) 1462
Bpu14I TTCGAA 2 cut(s) 942, 1523
BpuEI CTTGAG 3 cut(s) 647, 904, 1556
BpuMI CCSGG 3 cut(s) 286, 1962, 1963
BsaAI YACGTR 1 cut(s) 1775
BsaI GGTCTC 2 cut(s) 568, 1058
BsaJI CCNNGG 4 cut(s) 913, 1360, 1378, 1961
BsaWI WCCGGW 2 cut(s) 219, 906
BsaXI ACNNNNNCTCC 4 cut(s) 74, 104, 248, 278
Bsc4I CCNNNNNNNGG 8 cut(s) 99, 383, 422, 913, 1379, 1463, 1543, 1861
Bse1I ACTGG 5 cut(s) 377, 829, 1114, 1123, 1326
Bse21I CCTNAGG 1 cut(s) 165
Bse3DI GCAATG 2 cut(s) 854, 1195
BseBI CCWGG 3 cut(s) 327, 914, 1361
BseDI CCNNGG 4 cut(s) 913, 1360, 1378, 1961
BseGI GGATG 2 cut(s) 869, 2025
BseLI CCNNNNNNNGG 8 cut(s) 99, 383, 422, 913, 1379, 1463, 1543, 1861
BseMI GCAATG 2 cut(s) 854, 1195
BseMII CTCAG 7 cut(s) 179, 348, 988, 1293, 1476, 1848, 2024
BseNI ACTGG 5 cut(s) 377, 829, 1114, 1123, 1326
BseRI GAGGAG 7 cut(s) 150, 153, 210, 317, 962, 1880, 1943
BseSI GKGCMC 1 cut(s) 51
BseXI GCAGC 2 cut(s) 505, 2005
BsgI GTGCAG 1 cut(s) 1278
Bsh1236I CGCG 1 cut(s) 254
BshFI GGCC 1 cut(s) 1706
BshNI GGYRCC 1 cut(s) 487
BsiHKAI GWGCWC 5 cut(s) 51, 363, 1080, 1348, 1888
BsiHKCI CYCGRG 3 cut(s) 483, 873, 1961
BsiSI CCGG 4 cut(s) 220, 286, 907, 1962
BslI CCNNNNNNNGG 8 cut(s) 99, 383, 422, 913, 1379, 1463, 1543, 1861
BsmAI GTCTC 7 cut(s) 77, 385, 568, 647, 749, 870, 1058
BsmI GAATGC 5 cut(s) 229, 343, 1340, 1855, 2002
BsnI GGCC 1 cut(s) 1706
Bso31I GGTCTC 2 cut(s) 568, 1058
BsoBI CYCGRG 3 cut(s) 483, 873, 1961
Bsp119I TTCGAA 2 cut(s) 942, 1523
Bsp1286I GDGCHC 7 cut(s) 51, 363, 484, 768, 1080, 1348, 1888
BspACI CCGC 4 cut(s) 41, 58, 203, 254
BspANI GGCC 1 cut(s) 1706
BspCNI CTCAG 7 cut(s) 178, 349, 987, 1294, 1475, 1847, 2023
BspFNI CGCG 1 cut(s) 254
BspHI TCATGA 2 cut(s) 649, 751
BspLI GGNNCC 5 cut(s) 261, 489, 765, 911, 1468
BspMAI CTGCAG 2 cut(s) 523, 2023
BspPI GGATC 3 cut(s) 345, 1461, 1474
BspQI GCTCTTC 1 cut(s) 287
BspT104I TTCGAA 2 cut(s) 942, 1523
BspT107I GGYRCC 1 cut(s) 487
BspTI CTTAAG 1 cut(s) 863
BspTNI GGTCTC 2 cut(s) 568, 1058
BsrDI GCAATG 2 cut(s) 854, 1195
BsrI ACTGG 5 cut(s) 377, 829, 1114, 1123, 1326
BssECI CCNNGG 4 cut(s) 913, 1360, 1378, 1961
Bst2UI CCWGG 3 cut(s) 327, 914, 1361
Bst4CI ACNGT 3 cut(s) 646, 760, 1435
Bst6I CTCTTC 4 cut(s) 287, 1683, 1715, 2101
BstAFI CTTAAG 1 cut(s) 863
BstBAI YACGTR 1 cut(s) 1775
BstBI TTCGAA 2 cut(s) 942, 1523
BstC8I GCNNGC 3 cut(s) 1164, 1220, 2023
BstENI CCTNNNNNAGG 2 cut(s) 1377, 1859
BstF5I GGATG 2 cut(s) 869, 2025
BstFNI CGCG 1 cut(s) 254
BstH2I RGCGCY 1 cut(s) 402
BstHHI GCGC 2 cut(s) 229, 401
BstMAI GTCTC 7 cut(s) 77, 385, 568, 647, 749, 870, 1058
BstMWI GCNNNNNNNGC 1 cut(s) 55
BstNI CCWGG 3 cut(s) 327, 914, 1361
BstNSI RCATGY 1 cut(s) 1151
BstPAI GACNNNNGTC 1 cut(s) 1362
BstSCI CCNGG 6 cut(s) 284, 325, 912, 1359, 1960, 1961
BstSFI CTRYAG 5 cut(s) 519, 543, 1266, 1758, 2019
BstSLI GKGCMC 1 cut(s) 51
BstSNI TACGTA 1 cut(s) 1775
BstUI CGCG 1 cut(s) 254
BstV1I GCAGC 2 cut(s) 505, 2005
BstV2I GAAGAC 2 cut(s) 113, 129
BstX2I RGATCY 5 cut(s) 29, 337, 1234, 1466, 1873
BstYI RGATCY 5 cut(s) 29, 337, 1234, 1466, 1873
Bsu36I CCTNAGG 1 cut(s) 165
BsuRI GGCC 1 cut(s) 1706
BtsCI GGATG 2 cut(s) 869, 2025
BtsI GCAGTG 2 cut(s) 720, 1611
BtsIMutI CAGTG 3 cut(s) 720, 982, 1611
Cac8I GCNNGC 3 cut(s) 1164, 1220, 2023
CaiI CAGNNNCTG 1 cut(s) 383
CciI TCATGA 2 cut(s) 649, 751
CfoI GCGC 2 cut(s) 229, 401
Cfr13I GGNCC 2 cut(s) 155, 200
Cfr9I CCCGGG 1 cut(s) 1961
CviAII CATG 8 cut(s) 650, 685, 695, 752, 997, 1148, 1647, 1716
DraI TTTAAA 1 cut(s) 2070
DriI GACNNNNNGTC 1 cut(s) 304
Eam1104I CTCTTC 4 cut(s) 287, 1683, 1715, 2101
Eam1105I GACNNNNNGTC 1 cut(s) 304
EarI CTCTTC 4 cut(s) 287, 1683, 1715, 2101
Ecl136II GAGCTC 3 cut(s) 361, 1078, 1346
Eco105I TACGTA 1 cut(s) 1775
Eco24I GRGCYC 5 cut(s) 363, 484, 768, 1080, 1348
Eco31I GGTCTC 2 cut(s) 568, 1058
Eco32I GATATC 2 cut(s) 616, 1443
Eco47I GGWCC 2 cut(s) 155, 200
Eco53kI GAGCTC 3 cut(s) 361, 1078, 1346
Eco57I CTGAAG 4 cut(s) 92, 116, 1142, 1584
Eco81I CCTNAGG 1 cut(s) 165
Eco88I CYCGRG 3 cut(s) 483, 873, 1961
EcoICRI GAGCTC 3 cut(s) 361, 1078, 1346
EcoNI CCTNNNNNAGG 2 cut(s) 1377, 1859
EcoRI GAATTC 1 cut(s) 317
EcoRII CCWGG 3 cut(s) 325, 912, 1359
EcoRV GATATC 2 cut(s) 616, 1443
EcoT22I ATGCAT 2 cut(s) 1340, 1684
EcoT38I GRGCYC 5 cut(s) 363, 484, 768, 1080, 1348
FaeI CATG 8 cut(s) 653, 688, 698, 755, 1000, 1151, 1650, 1719
FalI AAGNNNNNCTT 2 cut(s) 1211, 1243
FatI CATG 8 cut(s) 649, 684, 694, 751, 996, 1147, 1646, 1715
FauI CCCGC 1 cut(s) 34
FauNDI CATATG 1 cut(s) 814
FblI GTMKAC 1 cut(s) 266
Fnu4HI GCNGC 3 cut(s) 59, 519, 2019
FokI GGATG 2 cut(s) 856, 2012
FriOI GRGCYC 5 cut(s) 363, 484, 768, 1080, 1348
Fsp4HI GCNGC 3 cut(s) 59, 519, 2019
FspBI CTAG 4 cut(s) 210, 537, 1736, 1989
FspI TGCGCA 1 cut(s) 228
GlaI GCGC 2 cut(s) 228, 400
GluI GCNGC 3 cut(s) 59, 519, 2019
GsuI CTGGAG 1 cut(s) 488
HaeII RGCGCY 1 cut(s) 402
HaeIII GGCC 1 cut(s) 1706
HapII CCGG 4 cut(s) 220, 286, 907, 1962
HhaI GCGC 2 cut(s) 229, 401
Hin1II CATG 8 cut(s) 653, 688, 698, 755, 1000, 1151, 1650, 1719
Hin6I GCGC 2 cut(s) 227, 399
HinP1I GCGC 2 cut(s) 227, 399
HincII GTYRAC 3 cut(s) 267, 670, 1633
HindII GTYRAC 3 cut(s) 267, 670, 1633
HindIII AAGCTT 2 cut(s) 430, 989
HpaII CCGG 4 cut(s) 220, 286, 907, 1962
HphI GGTGA 2 cut(s) 1433, 1446
Hpy99I CGWCG 2 cut(s) 268, 302
HpyCH4III ACNGT 3 cut(s) 646, 760, 1435
HpyCH4IV ACGT 1 cut(s) 1774
HpyF10VI GCNNNNNNNGC 1 cut(s) 55
HpySE526I ACGT 1 cut(s) 1774
Hsp92II CATG 8 cut(s) 653, 688, 698, 755, 1000, 1151, 1650, 1719
HspAI GCGC 2 cut(s) 227, 399
KpnI GGTACC 1 cut(s) 491
LguI GCTCTTC 1 cut(s) 287
LmnI GCTCC 4 cut(s) 265, 763, 909, 1075
Lsp1109I GCAGC 2 cut(s) 505, 2005
LweI GCATC 6 cut(s) 700, 878, 882, 1669, 1691, 2034
MaeI CTAG 4 cut(s) 210, 537, 1736, 1989
MaeII ACGT 1 cut(s) 1774
MaeIII GTNAC 5 cut(s) 640, 1421, 1551, 1857, 2102
MflI RGATCY 5 cut(s) 29, 337, 1234, 1466, 1873
MhlI GDGCHC 7 cut(s) 51, 363, 484, 768, 1080, 1348, 1888
MluCI AATT 6 cut(s) 280, 317, 698, 780, 1673, 2033
MlyI GAGTC 4 cut(s) 314, 324, 1418, 1700
MmeI TCCRAC 2 cut(s) 168, 811
Mph1103I ATGCAT 2 cut(s) 1340, 1684
MseI TTAA 7 cut(s) 582, 864, 1071, 1308, 1493, 1923, 2069
MspA1I CMGCKG 3 cut(s) 58, 925, 1972
MspCI CTTAAG 1 cut(s) 863
MspI CCGG 4 cut(s) 220, 286, 907, 1962
MspR9I CCNGG 6 cut(s) 286, 327, 914, 1361, 1962, 1963
Mva1269I GAATGC 5 cut(s) 229, 343, 1340, 1855, 2002
MvaI CCWGG 3 cut(s) 327, 914, 1361
MvnI CGCG 1 cut(s) 254
MwoI GCNNNNNNNGC 1 cut(s) 55
NciI CCSGG 3 cut(s) 286, 1962, 1963
NdeI CATATG 1 cut(s) 814
NlaIII CATG 8 cut(s) 653, 688, 698, 755, 1000, 1151, 1650, 1719
NlaIV GGNNCC 5 cut(s) 261, 489, 765, 911, 1468
NmuCI GTSAC 2 cut(s) 640, 1421
NsbI TGCGCA 1 cut(s) 228
NsiI ATGCAT 2 cut(s) 1340, 1684
NspI RCATGY 1 cut(s) 1151
NspV TTCGAA 2 cut(s) 942, 1523
PagI TCATGA 2 cut(s) 649, 751
PciSI GCTCTTC 1 cut(s) 287
PctI GAATGC 5 cut(s) 229, 343, 1340, 1855, 2002
PfeI GAWTC 6 cut(s) 440, 1133, 1409, 1577, 1936, 1966
PflFI GACNNNGTC 2 cut(s) 88, 1508
PflMI CCANNNNNTGG 1 cut(s) 383
PfoI TCCNGGA 2 cut(s) 284, 325
PkrI GCNGC 3 cut(s) 60, 520, 2020
PleI GAGTC 4 cut(s) 313, 324, 1418, 1699
PpsI GAGTC 4 cut(s) 313, 324, 1418, 1699
Ppu21I YACGTR 1 cut(s) 1775
PshAI GACNNNNGTC 1 cut(s) 1362
PshBI ATTAAT 1 cut(s) 1308
Psp124BI GAGCTC 3 cut(s) 363, 1080, 1348
Psp6I CCWGG 3 cut(s) 325, 912, 1359
PspGI CCWGG 3 cut(s) 325, 912, 1359
PspN4I GGNNCC 5 cut(s) 261, 489, 765, 911, 1468
PspPI GGNCC 2 cut(s) 155, 200
PstI CTGCAG 2 cut(s) 523, 2023
PstNI CAGNNNCTG 1 cut(s) 383
PsuI RGATCY 5 cut(s) 29, 337, 1234, 1466, 1873
PsyI GACNNNGTC 2 cut(s) 88, 1508
PvuII CAGCTG 2 cut(s) 925, 1972
SacI GAGCTC 3 cut(s) 363, 1080, 1348
SalI GTCGAC 1 cut(s) 265
SapI GCTCTTC 1 cut(s) 287
SaqAI TTAA 7 cut(s) 582, 864, 1071, 1308, 1493, 1923, 2069
SatI GCNGC 3 cut(s) 59, 519, 2019
Sau96I GGNCC 2 cut(s) 155, 200
ScaI AGTACT 1 cut(s) 1108
SchI GAGTC 4 cut(s) 314, 324, 1418, 1700
ScrFI CCNGG 6 cut(s) 286, 327, 914, 1361, 1962, 1963
SduI GDGCHC 7 cut(s) 51, 363, 484, 768, 1080, 1348, 1888
SfaNI GCATC 6 cut(s) 700, 878, 882, 1669, 1691, 2034
SfcI CTRYAG 5 cut(s) 519, 543, 1266, 1758, 2019
SfuI TTCGAA 2 cut(s) 942, 1523
SinI GGWCC 2 cut(s) 155, 200
SmaI CCCGGG 1 cut(s) 1963
SmlI CTYRAG 4 cut(s) 662, 863, 883, 1571
SmoI CTYRAG 4 cut(s) 662, 863, 883, 1571
SnaBI TACGTA 1 cut(s) 1775
Sse9I AATT 6 cut(s) 280, 317, 698, 780, 1673, 2033
SsiI CCGC 4 cut(s) 41, 58, 203, 254
SspI AATATT 1 cut(s) 1918
SspMI CTAG 4 cut(s) 210, 537, 1736, 1989
SstI GAGCTC 3 cut(s) 363, 1080, 1348
StyD4I CCNGG 6 cut(s) 284, 325, 912, 1359, 1960, 1961
TaaI ACNGT 3 cut(s) 646, 760, 1435
TaiI ACGT 1 cut(s) 1777
TaqI TCGA 4 cut(s) 266, 942, 982, 1523
TasI AATT 6 cut(s) 280, 317, 698, 780, 1673, 2033
TatI WGTACW 2 cut(s) 1106, 1743
TauI GCSGC 1 cut(s) 61
TfiI GAWTC 6 cut(s) 440, 1133, 1409, 1577, 1936, 1966
Tru1I TTAA 7 cut(s) 582, 864, 1071, 1308, 1493, 1923, 2069
Tru9I TTAA 7 cut(s) 582, 864, 1071, 1308, 1493, 1923, 2069
TscAI CASTG 3 cut(s) 720, 982, 1618
TseFI GTSAC 2 cut(s) 640, 1421
TseI GCWGC 2 cut(s) 518, 2018
Tsp45I GTSAC 2 cut(s) 640, 1421
TspGWI ACGGA 3 cut(s) 21, 252, 1409
TspMI CCCGGG 1 cut(s) 1961
TspRI CASTG 3 cut(s) 720, 982, 1618
Tth111I GACNNNGTC 2 cut(s) 88, 1508
Van91I CCANNNNNTGG 1 cut(s) 383
Vha464I CTTAAG 1 cut(s) 863
VneI GTGCAC 1 cut(s) 47
VpaK11BI GGWCC 2 cut(s) 155, 200
VspI ATTAAT 1 cut(s) 1308
XagI CCTNNNNNAGG 2 cut(s) 1377, 1859
XapI RAATTY 3 cut(s) 317, 698, 1673
XbaI TCTAGA 2 cut(s) 209, 1988
XceI RCATGY 1 cut(s) 1151
XmaI CCCGGG 1 cut(s) 1961
XmiI GTMKAC 1 cut(s) 266
XspI CTAG 4 cut(s) 210, 537, 1736, 1989
ZrmI AGTACT 1 cut(s) 1108
Zsp2I ATGCAT 2 cut(s) 1340, 1684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.