MD09G1226100.v1.1

Multiple C2 and transmembrane domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014894
Physical Location & Seq
Forward (+)
27895146 .. 27902847
7702 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1226100.v1.1.491

Sequence Viewer

Length: 2796 bp
ATGATTCATGAGATGGCCAAAGTCATAACTTGCAGAAATGCAGTCATTCTATATAGTTACCATTATAGGACCAACCAGAAGATCTATTTAGCTTTAGATGTGGTTTTTTCCTTCTCGGATGACGGATTAGATGCGATTGGCTGGTTCTCAACAGATGCAGCTGAATTAACAGATGAGTGTGATGATGTGCTAATGGAAAGGAAGATGAACAAAGTAGTCAACCAAAAGAAAGTAGAAGGGTTTGGAAACAATATGAAGAAGATGCATTGCTGTCCATACTTGAGGAGGCAGTTGTTAAGAATTTGCGATGTGATAATGGTTGTTTCGAACCCGGAACAATGGCTCAGTTTAAACTTGGAGCTCCCAAGCTATTCAAGCATTTGTATATTGTATGAACTTGGATATGTGTGCGAAGAAATGGCTAAGCAGAACACAGCAAAAGAAGATTTCTCCCTAAAGGAGACCTCGCCGAACATCACTGGCCGGAGGTTCTCCATGGGTCCCATGACCTCCTTTGATCTTGTCGAGCACATGCAGTATCTCTATGTCAGGATTGTGAAAGCCAGAGATTTGCCACCTACTGCCGACCCTTATGTTGAACTGAAGCTCGGAAACTACCGGGCAGTAACAACACCTTTCAAAAAAAATCCGAACCCCGAATGGAACCAAGTGTTCGCTTTTTCGAAAGACAGAATTCAAGCTGTTAGTATGGAGATTTTGGTGAAGGACAAGGCTGTTGTTGCAGAAGGTGGTGATCATGGAACCATTGGGATGTTTGGTTTTGCTATGGCTGAGTGTCCTAGTAGGGTTCCGCCGGATAGTCCGTTAGCGCCGCAATGGTACATGCTGGAGGATCAAAACAAGGCCAAGATTAGAGGAGACCTAATGCTTTCTTATTGGATTGGGACGCAAGCAGATGAGGCCTTTCCAGATGCTTGGCACGCTGATGTTGCATCGGTAAGTGGGGATGGACTTTCGAGTACTCGTTCGAAGGTTTACCTTTCACCTAGGCTTTGGTACATGAGAGTGAATGTGATTCAAGCTCAGGATCTTGTGTTAAAAGACAACAACAGAAAGCAAGCACCGGAGTTTTTTGTGAAGGCTCAGTTCGGGAATGTGGTTTTGAGAAGTGGAGTCTCGACGGATAAGAATTTCAACCCTACTTGGAATGAGGATCTAATGTTTGTTGTTGCAGAACCGTTCGATGATCCTTTGGTGATAAGTGTTCAGGAGAAAACGAATAACAAGGAGGAATCTGCCGGGCAGATTGTGATTCCTTTGAGGGATGTGGATAAGAGGATAGACGCTACTCCGGCTACTCCCAAGTGGTATAATCTTGGGACGGTTGAAGTAGTTGAGGGTGTGCCAAAGGAGGTGAAGTTTGCGAGTAAGATCCAAATAAGGGTGAGTTTGGATGGAGGATATCATGTTCTTGACGAGCCAGCTCACAGTACTAGTGATCTGAGGCCAACTGCAAAGCTTCTTTGGAGGCCTCCGATCGGGATTTTGGAATTGGGAATCTTGAATGCAACTGGTTTGTCACCAATGAAGCCTAAGAATCGTGTTGACGCATATTGTGTAGCAAAATATGGGACAAAGTGGGTGCGAGCAAGGACGGTTGTTGATTGTTTGTCTCCCAAGTGGAACGAACAATACACATGGGAAGTCTATGATCCATGCACTGTCATTACCATTGGAGTTTTTGACAATGGGAACTTGCAGGGTGGCGATAAGCTGGGCATGGATTTGAGTATTGGGAAGGTAAAGATCCGTCTATCTACCCTTGAAACTGGTAGGATTTACACGCATTCATATCCTCTTGTGGTTCTACAACCTACTGGGGTGAAAAAGATGGGTGAAATCCAACTGGCTCTGAGATTTTCGTGTCCGTCTTTGCCTAACATGCTGCTCACATACACACGGCCTCTGCTGCCTAAGATGCACTACATTATCCCACTGTCTATATACCAGCTTGCTAGTTTACGACACCAAGCTGCATTGATTCTCTGGTTGAGGTTGAGCCGGGCTGAGCCACCACTTAGGAGAGAGGTTGTGGATTACATGTTAGATTCCACTGCGCATCTGTGGAGTTTTCGAAGAGGCAAGGCCAATTTTGTCAGGATCATCAAGCTATTTGATGGTTTGGTCATGATGTTCAAAGGGTTTGATAAAATACGGAAATGGACTAACACCATTACCACGGTGCTTGTTCACGTTGCCTTCACGGTCATGCTTTTTTTTCCTGGTCCGACAATTTGCTTAATGTTTTTATTGCTGTTGCTGAAAGGGGTTTGGAATTACCGGAAGAGGCCTAGACAAATTGTGCACATAGACACAGAACTGTCACAAGCATATGGCGTACATCCAGAGGATTTGGACGAAGAGTTTGATACTTTTCCAACAAAAAAGACGGGCGATGTTCTGAAACGAAGATATGATCGTCTTCGAGGCATTGCAGGGAGGATTCAGGCAGTGTTGGGGGACATAGCGACACAAGGCGAAAGGGTGCAGTCTCTGCTGAGTTGGAGGGATCCAAGAGCCACAACTTTATTTATGATCTTCTGTATTATTGGTATGATCCTATTTTATGTCTTCTCGTTGAAGTTGATAGTTTGGCTTGCAGGATTTTATGCAATGAGACATCCAAAATGTCGGGATAGATTCCCTTCGCTCCTGCAAAACTTGATCAGGAGGTTGCCGGCAAGATCAGATAGCATGTTTTGGCATATGTTGATCATGTTCTCTAATAAAGATGATTTGTATGCTATCAGAAACACTTCTAGCTATGTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 PRT_C

Protein Analysis

932

Amino Acids

106.46

Weight (kDa)

8.97

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 180 - 281 7e-12 C2 domain
C2 PF00168 340 - 446 3.1e-16 C2 domain
C2 PF00168 501 - 607 5.4e-19 C2 domain
PRT_C PF08372 755 - 907 9.7e-62 Plant phosphoribosyltransferase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014894)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20080
fragaria_vesca FvH4_3g14700
malus_domestica MD09G1226100.v1.1 MD11G1015900.v1.1
prunus_persica Prupe.4G132300_v2.0.a1 Prupe.6G014000_v2.0.a1
pyrus_communis pycom11g01110
rosa_chinensis RchiOBHm_Chr5g0024191
rosa_laevigata RLG00000032778
rosa_multiflora Rmu_sc0004033.1_g000007
rosa_rugosa Rorug05G0082500
rosa_samantha Rh5AG173000 Rh5BG169700 Rh5CG185500 Rh5DG172000
rosa_wichuraiana Rw5G015610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2079
AciI CCGC 2 cut(s) 812, 833
AcoI YGGCCR 2 cut(s) 15, 481
AcsI RAATTY 3 cut(s) 300, 693, 1150
AcuI CTGAAG 1 cut(s) 623
AfaI GTAC 5 cut(s) 842, 982, 1019, 1453, 2361
AfiI CCNNNNNNNGG 2 cut(s) 1402, 1499
AflIII ACRYGT 1 cut(s) 2061
AhlI ACTAGT 1 cut(s) 1454
AjnI CCWGG 1 cut(s) 2242
AloI GAACNNNNNNTCC 2 cut(s) 1413, 1445
Alw21I GWGCWC 3 cut(s) 363, 531, 2328
Alw26I GTCTC 6 cut(s) 455, 873, 1141, 1638, 2517, 2632
Alw44I GTGCAC 1 cut(s) 2324
AlwNI CAGNNNCTG 1 cut(s) 2515
AoxI GGCC 9 cut(s) 15, 481, 864, 921, 1466, 1490, 1922, 2106, 2309
ApaLI GTGCAC 1 cut(s) 2324
ApeKI GCWGC 4 cut(s) 158, 1906, 1930, 1994
ApoI RAATTY 3 cut(s) 300, 693, 1150
ArsI GACNNNNNNTTYG 2 cut(s) 2369, 2401
Asp700I GAANNNNTTC 1 cut(s) 2776
AspA2I CCTAGG 1 cut(s) 1007
AspLEI GCGC 2 cut(s) 832, 2080
AspS9I GGNCC 3 cut(s) 69, 500, 2246
AsuC2I CCSGG 4 cut(s) 332, 620, 1261, 2024
AsuHPI GGTGA 9 cut(s) 733, 764, 996, 1228, 1387, 1417, 1533, 1855, 1868
AsuII TTCGAA 4 cut(s) 326, 683, 989, 2095
AvaII GGWCC 3 cut(s) 69, 500, 2246
AvrII CCTAGG 1 cut(s) 1007
BaeGI GKGCMC 1 cut(s) 2328
BalI TGGCCA 1 cut(s) 17
BamHI GGATCC 1 cut(s) 2530
BanII GRGCYC 1 cut(s) 363
BbsI GAAGAC 2 cut(s) 2435, 2584
Bbv12I GWGCWC 3 cut(s) 363, 531, 2328
BbvI GCAGC 4 cut(s) 170, 1893, 1917, 1981
BccI CCATC 5 cut(s) 7, 962, 1409, 1846, 2132
BceAI ACGGC 1 cut(s) 1937
BciT130I CCWGG 1 cut(s) 2244
BclI TGATCA 3 cut(s) 754, 2685, 2733
BcnI CCSGG 4 cut(s) 332, 620, 1261, 2024
BcoDI GTCTC 6 cut(s) 455, 873, 1141, 1638, 2517, 2632
BcuI ACTAGT 1 cut(s) 1454
BfaI CTAG 6 cut(s) 801, 1008, 1455, 1977, 2313, 2781
BfoI RGCGCY 1 cut(s) 833
BglII AGATCT 1 cut(s) 81
BisI GCNGC 5 cut(s) 159, 833, 1907, 1931, 1995
BlnI CCTAGG 1 cut(s) 1007
BlpI GCTNAGC 2 cut(s) 423, 2028
BlsI GCNGC 5 cut(s) 160, 834, 1908, 1932, 1996
BmcAI AGTACT 2 cut(s) 982, 1453
Bme1390I CCNGG 5 cut(s) 332, 620, 1261, 2024, 2244
Bme18I GGWCC 3 cut(s) 69, 500, 2246
BmgT120I GGNCC 3 cut(s) 69, 500, 2246
BmiI GGNNCC 6 cut(s) 501, 502, 665, 763, 810, 2532
BmrFI CCNGG 5 cut(s) 332, 620, 1261, 2024, 2244
BmrI ACTGGG 1 cut(s) 1848
BmsI GCATC 7 cut(s) 121, 145, 252, 922, 962, 1929, 2089
BmuI ACTGGG 1 cut(s) 1848
BpiI GAAGAC 2 cut(s) 2435, 2584
BpmI CTGGAG 1 cut(s) 869
Bpu10I CCTNAGC 1 cut(s) 1044
Bpu1102I GCTNAGC 2 cut(s) 423, 2028
Bpu14I TTCGAA 4 cut(s) 326, 683, 989, 2095
BpuEI CTTGAG 1 cut(s) 301
BpuMI CCSGG 4 cut(s) 332, 620, 1261, 2024
BsaI GGTCTC 2 cut(s) 455, 873
BsaJI CCNNGG 3 cut(s) 495, 1007, 2199
BsaWI WCCGGW 2 cut(s) 1084, 2301
BsaXI ACNNNNNCTCC 2 cut(s) 1364, 1394
Bsc4I CCNNNNNNNGG 2 cut(s) 1402, 1499
Bse118I RCCGGY 1 cut(s) 2698
Bse1I ACTGG 5 cut(s) 484, 1537, 1795, 1843, 1872
Bse3DI GCAATG 4 cut(s) 265, 842, 2451, 2640
BseBI CCWGG 1 cut(s) 2244
BseDI CCNNGG 3 cut(s) 495, 1007, 2199
BseGI GGATG 7 cut(s) 124, 777, 973, 1291, 1420, 2362, 2641
BseLI CCNNNNNNNGG 2 cut(s) 1402, 1499
BseMI GCAATG 4 cut(s) 265, 842, 2451, 2640
BseMII CTCAG 8 cut(s) 358, 783, 1058, 1118, 1454, 1865, 2019, 2510
BseNI ACTGG 5 cut(s) 484, 1537, 1795, 1843, 1872
BseRI GAGGAG 2 cut(s) 298, 891
BseSI GKGCMC 1 cut(s) 2328
BseXI GCAGC 4 cut(s) 170, 1893, 1917, 1981
BseYI CCCAGC 1 cut(s) 1735
BsgI GTGCAG 1 cut(s) 2528
Bsh1285I CGRYCG 1 cut(s) 1500
BshFI GGCC 9 cut(s) 17, 483, 866, 923, 1468, 1492, 1924, 2108, 2311
BsiEI CGRYCG 1 cut(s) 1500
BsiHKAI GWGCWC 3 cut(s) 363, 531, 2328
BslFI GGGAC 5 cut(s) 486, 919, 1354, 1606, 2495
BslI CCNNNNNNNGG 2 cut(s) 1402, 1499
BsmAI GTCTC 6 cut(s) 455, 873, 1141, 1638, 2517, 2632
BsmFI GGGAC 5 cut(s) 486, 919, 1354, 1606, 2495
BsmI GAATGC 2 cut(s) 1531, 1807
BsnI GGCC 9 cut(s) 17, 483, 866, 923, 1468, 1492, 1924, 2108, 2311
Bso31I GGTCTC 2 cut(s) 455, 873
Bsp119I TTCGAA 4 cut(s) 326, 683, 989, 2095
Bsp1286I GDGCHC 3 cut(s) 363, 531, 2328
Bsp1720I GCTNAGC 2 cut(s) 423, 2028
Bsp19I CCATGG 1 cut(s) 495
BspACI CCGC 2 cut(s) 812, 833
BspANI GGCC 9 cut(s) 17, 483, 866, 923, 1468, 1492, 1924, 2108, 2311
BspCNI CTCAG 8 cut(s) 357, 784, 1057, 1117, 1455, 1866, 2020, 2511
BspHI TCATGA 2 cut(s) 7, 2148
BspLI GGNNCC 6 cut(s) 501, 502, 665, 763, 810, 2532
BspT104I TTCGAA 4 cut(s) 326, 683, 989, 2095
BspTNI GGTCTC 2 cut(s) 455, 873
BsrDI GCAATG 4 cut(s) 265, 842, 2451, 2640
BsrFI RCCGGY 1 cut(s) 2698
BsrI ACTGG 5 cut(s) 484, 1537, 1795, 1843, 1872
BssAI RCCGGY 1 cut(s) 2698
BssECI CCNNGG 3 cut(s) 495, 1007, 2199
BssT1I CCWWGG 2 cut(s) 495, 1007
Bst2UI CCWGG 1 cut(s) 2244
Bst4CI ACNGT 9 cut(s) 1200, 1345, 1451, 1618, 1684, 1959, 2203, 2227, 2343
Bst6I CTCTTC 3 cut(s) 2092, 2300, 2376
BstAPI GCANNNNNTGC 1 cut(s) 2515
BstBI TTCGAA 4 cut(s) 326, 683, 989, 2095
BstC8I GCNNGC 8 cut(s) 912, 942, 1080, 1443, 1608, 1974, 2619, 2700
BstDSI CCRYGG 2 cut(s) 495, 2199
BstF5I GGATG 7 cut(s) 124, 777, 973, 1291, 1420, 2362, 2641
BstH2I RGCGCY 1 cut(s) 833
BstHHI GCGC 2 cut(s) 832, 2080
BstMAI GTCTC 6 cut(s) 455, 873, 1141, 1638, 2517, 2632
BstMCI CGRYCG 1 cut(s) 1500
BstNI CCWGG 1 cut(s) 2244
BstNSI RCATGY 5 cut(s) 535, 847, 1906, 2065, 2719
BstSCI CCNGG 5 cut(s) 330, 618, 1259, 2022, 2242
BstSLI GKGCMC 1 cut(s) 2328
BstV1I GCAGC 4 cut(s) 170, 1893, 1917, 1981
BstV2I GAAGAC 2 cut(s) 2435, 2584
BstX2I RGATCY 6 cut(s) 81, 1048, 1174, 1392, 1767, 2530
BstXI CCANNNNNNTGG 1 cut(s) 936
BstYI RGATCY 6 cut(s) 81, 1048, 1174, 1392, 1767, 2530
BsuRI GGCC 9 cut(s) 17, 483, 866, 923, 1468, 1492, 1924, 2108, 2311
BtgI CCRYGG 2 cut(s) 495, 2199
BtgZI GCGATG 2 cut(s) 321, 2430
BtsCI GGATG 7 cut(s) 124, 777, 973, 1291, 1420, 2362, 2641
BtsI GCAGTG 2 cut(s) 2073, 2478
BtsIMutI CAGTG 5 cut(s) 477, 1680, 1955, 2073, 2478
Cac8I GCNNGC 8 cut(s) 912, 942, 1080, 1443, 1608, 1974, 2619, 2700
CaiI CAGNNNCTG 1 cut(s) 2515
CciI TCATGA 2 cut(s) 7, 2148
CfoI GCGC 2 cut(s) 832, 2080
Cfr10I RCCGGY 1 cut(s) 2698
Cfr13I GGNCC 3 cut(s) 69, 500, 2246
CseI GACGC 3 cut(s) 916, 1313, 1577
Csp6I GTAC 5 cut(s) 841, 981, 1018, 1452, 2360
CspCI CAANNNNNGTGG 2 cut(s) 2188, 2223
CviQI GTAC 5 cut(s) 841, 981, 1018, 1452, 2360
DraI TTTAAA 1 cut(s) 351
EaeI YGGCCR 2 cut(s) 15, 481
Eam1104I CTCTTC 3 cut(s) 2092, 2300, 2376
EarI CTCTTC 3 cut(s) 2092, 2300, 2376
EciI GGCGGA 1 cut(s) 801
Ecl136II GAGCTC 1 cut(s) 361
Eco130I CCWWGG 2 cut(s) 495, 1007
Eco147I AGGCCT 3 cut(s) 923, 1492, 2311
Eco24I GRGCYC 1 cut(s) 363
Eco31I GGTCTC 2 cut(s) 455, 873
Eco32I GATATC 1 cut(s) 1424
Eco47I GGWCC 3 cut(s) 69, 500, 2246
Eco53kI GAGCTC 1 cut(s) 361
Eco57I CTGAAG 1 cut(s) 623
EcoICRI GAGCTC 1 cut(s) 361
EcoO109I RGGNCCY 1 cut(s) 500
EcoRI GAATTC 1 cut(s) 693
EcoRII CCWGG 1 cut(s) 2242
EcoRV GATATC 1 cut(s) 1424
EcoT14I CCWWGG 2 cut(s) 495, 1007
EcoT22I ATGCAT 1 cut(s) 267
EcoT38I GRGCYC 1 cut(s) 363
ErhI CCWWGG 2 cut(s) 495, 1007
FaqI GGGAC 5 cut(s) 486, 919, 1354, 1606, 2495
FauNDI CATATG 2 cut(s) 2353, 2727
FbaI TGATCA 3 cut(s) 754, 2685, 2733
Fnu4HI GCNGC 5 cut(s) 159, 833, 1907, 1931, 1995
FokI GGATG 7 cut(s) 131, 784, 980, 1298, 1427, 2349, 2628
FriOI GRGCYC 1 cut(s) 363
Fsp4HI GCNGC 5 cut(s) 159, 833, 1907, 1931, 1995
FspBI CTAG 6 cut(s) 801, 1008, 1455, 1977, 2313, 2781
FspI TGCGCA 1 cut(s) 2079
GlaI GCGC 2 cut(s) 831, 2079
GluI GCNGC 5 cut(s) 159, 833, 1907, 1931, 1995
GsaI CCCAGC 1 cut(s) 1739
GsuI CTGGAG 1 cut(s) 869
HaeII RGCGCY 1 cut(s) 833
HaeIII GGCC 9 cut(s) 17, 483, 866, 923, 1468, 1492, 1924, 2108, 2311
HgaI GACGC 3 cut(s) 916, 1313, 1577
HhaI GCGC 2 cut(s) 832, 2080
Hin6I GCGC 2 cut(s) 830, 2078
HinP1I GCGC 2 cut(s) 830, 2078
HincII GTYRAC 2 cut(s) 220, 1567
HindII GTYRAC 2 cut(s) 220, 1567
HindIII AAGCTT 1 cut(s) 1478
HphI GGTGA 9 cut(s) 733, 764, 996, 1228, 1387, 1417, 1533, 1855, 1868
Hpy166II GTNNAC 6 cut(s) 220, 997, 1567, 1982, 2212, 2326
Hpy8I GTNNAC 6 cut(s) 220, 997, 1567, 1982, 2212, 2326
Hpy99I CGWCG 1 cut(s) 1144
HpyAV CCTTC 9 cut(s) 121, 230, 718, 740, 985, 1093, 1753, 2230, 2676
HpyCH4III ACNGT 9 cut(s) 1200, 1345, 1451, 1618, 1684, 1959, 2203, 2227, 2343
HpyCH4IV ACGT 1 cut(s) 2214
HpySE526I ACGT 1 cut(s) 2214
HspAI GCGC 2 cut(s) 830, 2078
KflI GGGWCCC 1 cut(s) 500
KroI GCCGGC 1 cut(s) 2698
KroNI GCCGGC 1 cut(s) 2700
Ksp22I TGATCA 3 cut(s) 754, 2685, 2733
LmnI GCTCC 3 cut(s) 358, 366, 2676
Lsp1109I GCAGC 4 cut(s) 170, 1893, 1917, 1981
LweI GCATC 7 cut(s) 121, 145, 252, 922, 962, 1929, 2089
MaeI CTAG 6 cut(s) 801, 1008, 1455, 1977, 2313, 2781
MaeII ACGT 1 cut(s) 2214
MaeIII GTNAC 4 cut(s) 56, 625, 1539, 2343
MflI RGATCY 6 cut(s) 81, 1048, 1174, 1392, 1767, 2530
MhlI GDGCHC 3 cut(s) 363, 531, 2328
MlsI TGGCCA 1 cut(s) 17
MluCI AATT 9 cut(s) 164, 300, 693, 1150, 1511, 2110, 2253, 2296, 2319
MluNI TGGCCA 1 cut(s) 17
MlyI GAGTC 1 cut(s) 1143
MmeI TCCRAC 4 cut(s) 1888, 2273, 2423, 2504
Mox20I TGGCCA 1 cut(s) 17
Mph1103I ATGCAT 1 cut(s) 267
MroNI GCCGGC 1 cut(s) 2698
MroXI GAANNNNTTC 1 cut(s) 2776
MscI TGGCCA 1 cut(s) 17
MseI TTAA 5 cut(s) 167, 296, 350, 1058, 2261
MslI CAYNNNNRTG 4 cut(s) 770, 945, 1025, 2228
Msp20I TGGCCA 1 cut(s) 17
MspA1I CMGCKG 1 cut(s) 161
MspR9I CCNGG 5 cut(s) 332, 620, 1261, 2024, 2244
MssI GTTTAAAC 1 cut(s) 351
Mva1269I GAATGC 2 cut(s) 1531, 1807
MvaI CCWGG 1 cut(s) 2244
NaeI GCCGGC 1 cut(s) 2700
NciI CCSGG 4 cut(s) 332, 620, 1261, 2024
NcoI CCATGG 1 cut(s) 495
NdeI CATATG 2 cut(s) 2353, 2727
NgoMIV GCCGGC 1 cut(s) 2698
NlaIV GGNNCC 6 cut(s) 501, 502, 665, 763, 810, 2532
NmuCI GTSAC 2 cut(s) 1539, 2343
NsbI TGCGCA 1 cut(s) 2079
NsiI ATGCAT 1 cut(s) 267
NspI RCATGY 5 cut(s) 535, 847, 1906, 2065, 2719
NspV TTCGAA 4 cut(s) 326, 683, 989, 2095
PagI TCATGA 2 cut(s) 7, 2148
PceI AGGCCT 3 cut(s) 923, 1492, 2311
PciI ACATGT 1 cut(s) 2061
PctI GAATGC 2 cut(s) 1531, 1807
PdiI GCCGGC 1 cut(s) 2700
PdmI GAANNNNTTC 1 cut(s) 2776
PkrI GCNGC 5 cut(s) 160, 834, 1908, 1932, 1996
Ple19I CGATCG 1 cut(s) 1500
PleI GAGTC 1 cut(s) 1142
PmeI GTTTAAAC 1 cut(s) 351
PpsI GAGTC 1 cut(s) 1142
PpuMI RGGWCCY 1 cut(s) 500
PscI ACATGT 1 cut(s) 2061
Psp124BI GAGCTC 1 cut(s) 363
Psp5II RGGWCCY 1 cut(s) 500
Psp6I CCWGG 1 cut(s) 2242
PspFI CCCAGC 1 cut(s) 1735
PspGI CCWGG 1 cut(s) 2242
PspN4I GGNNCC 6 cut(s) 501, 502, 665, 763, 810, 2532
PspPI GGNCC 3 cut(s) 69, 500, 2246
PspPPI RGGWCCY 1 cut(s) 500
PsrI GAACNNNNNNTAC 2 cut(s) 1637, 1669
PstNI CAGNNNCTG 1 cut(s) 2515
PsuI RGATCY 6 cut(s) 81, 1048, 1174, 1392, 1767, 2530
PvuI CGATCG 1 cut(s) 1500
PvuII CAGCTG 1 cut(s) 161
RsaI GTAC 5 cut(s) 842, 982, 1019, 1453, 2361
RsaNI GTAC 5 cut(s) 841, 981, 1018, 1452, 2360
RseI CAYNNNNRTG 4 cut(s) 770, 945, 1025, 2228
SacI GAGCTC 1 cut(s) 363
SaqAI TTAA 5 cut(s) 167, 296, 350, 1058, 2261
SatI GCNGC 5 cut(s) 159, 833, 1907, 1931, 1995
Sau96I GGNCC 3 cut(s) 69, 500, 2246
ScaI AGTACT 2 cut(s) 982, 1453
SchI GAGTC 1 cut(s) 1143
ScrFI CCNGG 5 cut(s) 332, 620, 1261, 2024, 2244
SduI GDGCHC 3 cut(s) 363, 531, 2328
SfaNI GCATC 7 cut(s) 121, 145, 252, 922, 962, 1929, 2089
SfuI TTCGAA 4 cut(s) 326, 683, 989, 2095
SinI GGWCC 3 cut(s) 69, 500, 2246
SmiMI CAYNNNNRTG 4 cut(s) 770, 945, 1025, 2228
SmlI CTYRAG 1 cut(s) 280
SmoI CTYRAG 1 cut(s) 280
SpeI ACTAGT 1 cut(s) 1454
Sse9I AATT 9 cut(s) 164, 300, 693, 1150, 1511, 2110, 2253, 2296, 2319
SseBI AGGCCT 3 cut(s) 923, 1492, 2311
SsiI CCGC 2 cut(s) 812, 833
SspMI CTAG 6 cut(s) 801, 1008, 1455, 1977, 2313, 2781
SstI GAGCTC 1 cut(s) 363
StuI AGGCCT 3 cut(s) 923, 1492, 2311
StyD4I CCNGG 5 cut(s) 330, 618, 1259, 2022, 2242
StyI CCWWGG 2 cut(s) 495, 1007
TaaI ACNGT 9 cut(s) 1200, 1345, 1451, 1618, 1684, 1959, 2203, 2227, 2343
TaiI ACGT 1 cut(s) 2217
TaqI TCGA 9 cut(s) 326, 525, 683, 977, 989, 1139, 1203, 2095, 2446
TasI AATT 9 cut(s) 164, 300, 693, 1150, 1511, 2110, 2253, 2296, 2319
TatI WGTACW 2 cut(s) 980, 1451
TauI GCSGC 1 cut(s) 835
Tru1I TTAA 5 cut(s) 167, 296, 350, 1058, 2261
Tru9I TTAA 5 cut(s) 167, 296, 350, 1058, 2261
TscAI CASTG 5 cut(s) 484, 1687, 1962, 2080, 2478
TseFI GTSAC 2 cut(s) 1539, 2343
TseI GCWGC 4 cut(s) 158, 1906, 1930, 1994
Tsp45I GTSAC 2 cut(s) 1539, 2343
TspDTI ATGAA 5 cut(s) 221, 269, 408, 1562, 1800
TspGWI ACGGA 6 cut(s) 138, 813, 1157, 1760, 1878, 2191
TspRI CASTG 5 cut(s) 484, 1687, 1962, 2080, 2478
VneI GTGCAC 1 cut(s) 2324
VpaK11BI GGWCC 3 cut(s) 69, 500, 2246
XapI RAATTY 3 cut(s) 300, 693, 1150
XceI RCATGY 5 cut(s) 535, 847, 1906, 2065, 2719
XmaJI CCTAGG 1 cut(s) 1007
XmnI GAANNNNTTC 1 cut(s) 2776
XspI CTAG 6 cut(s) 801, 1008, 1455, 1977, 2313, 2781
ZrmI AGTACT 2 cut(s) 982, 1453
Zsp2I ATGCAT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.