MD09G1277800.v1.1

Plastocyanin-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0021132
Physical Location & Seq
Reverse (-)
35443036 .. 35444790
1755 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1277800.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGCATGCATGCATGCTCGTTTTTCAAGATCGCAAAATGCAGGGAATGAAGGAGATCATTGTGGTGGCGGTGATGTTCTCATCAATGTTATTCCGCTGCGGGGTCTCTGCTAGTACTATGGCGACCGCTGCTGCAACCAACACCAACCACTCTGTTGGCGGAGCCTCCGGTTGGGATCTCTCCTCCAACCTCCGAGCATGGGCTACTCGCACCGCTTTCCGTGTGGGCGACTCTCTCGTATTCAGGTACACTCCGGTGCACGATGTACTGGAAGTGACGAAGACGGACTATGATTTGTGTCACACAGTAGACCCACTGGAGATGCACAACGATGGGGAGACGGTGATTCCATTGAGGGAAGCAGGAAACAGGTACTTCATATGTGGGAGGCTGGGCCATTGTGCCATGGGACTCAAGCTCCATGTGCTTGTCCTCCCTGCACTGCAGATGAGTACCAATGTTAATGCAACCTCACCCAGCAGCCCCTTGTTCACTAGTCCTCCGCCAAATGCTACTAGTCCTATTTCCAACAACAACAGCAGCAGACACGGTCCAGATGATCAAGTAGTGGGAAATGATCATGTGCAGCCTTCCCCATCGCCATCGCTATCTGCTTCCAATTACTCAATTAATTCTACAGATGATGTACCTCCTGATCATGCTACAGGCAATACTACTACTGATGATGCACACTTAACTTCTGACAGCCCTCGGATTCTGCCATTTGTTACAGTGATCAGGCAGATCCTCGTGTTGGCCATTTTTTTATTTCCGAGTTGGATTGCCGCCTGGCCCGCCTCCATAGCTCCGTTTGTTCCCTTCACTCATGTTGCTCTGATTCTTCTCGTCGCTACTATCACTGTTGTTGCTGTTGAGTTACTTCTCACTACTAGGCCAACTTCGGACAACAACAGCAGACATGGTCCCGATAATCAAGTAGTGAAAAATGATCGTGTGCTGCCTTCTCCTCCGTCAGCTGCTTCCAATTACTCTATTCATTCTACAGATGATGCACCTCCTGATCATGCTACAGGCAATACAACTACTTCTGATGCCCAATTAACTGCGAAGATCCCTTGGATTCGGCCATTGGTAAGAATAGTTGTAGTACTCATGATCGCACATATCCTCATGTATCCTCTTCTTCATTTACTAGTTGGATCACCGCCTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

392

Amino Acids

42.15

Weight (kDa)

6.15

Isoelectric Point (pI)

46.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 61 - 137 3.2e-24 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021132)

Species Orthologous Gene IDs
malus_domestica MD06G1238600.v1.1 MD09G1277800.v1.1
prunus_persica Prupe.5G242500_v2.0.a1
pyrus_communis pycom06g21410 pycom09g18720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 309
AclWI GGATC 4 cut(s) 183, 739, 1066, 1168
AcoI YGGCCR 2 cut(s) 756, 1085
AfaI GTAC 7 cut(s) 115, 248, 267, 374, 454, 648, 1110
AfiI CCNNNNNNNGG 4 cut(s) 100, 171, 199, 754
AgsI TTSAA 1 cut(s) 26
AhlI ACTAGT 3 cut(s) 494, 515, 1153
AjnI CCWGG 1 cut(s) 788
AleI CACNNNNGTG 1 cut(s) 254
AluBI AGCT 3 cut(s) 418, 806, 977
AluI AGCT 3 cut(s) 418, 806, 977
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 2 cut(s) 109, 332
Alw44I GTGCAC 1 cut(s) 257
AlwI GGATC 4 cut(s) 183, 739, 1066, 1168
AoxI GGCC 5 cut(s) 394, 756, 791, 893, 1085
ApaLI GTGCAC 1 cut(s) 257
ApeKI GCWGC 8 cut(s) 96, 128, 131, 480, 540, 586, 958, 977
AseI ATTAAT 1 cut(s) 630
AspS9I GGNCC 4 cut(s) 394, 551, 792, 923
AsuHPI GGTGA 4 cut(s) 82, 355, 465, 1155
AvaII GGWCC 2 cut(s) 551, 923
BaeGI GKGCMC 1 cut(s) 261
BalI TGGCCA 1 cut(s) 758
BauI CACGAG 1 cut(s) 749
BbsI GAAGAC 1 cut(s) 287
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 8 cut(s) 83, 115, 118, 492, 552, 598, 945, 964
BccI CCATC 3 cut(s) 326, 604, 610
BciT130I CCWGG 1 cut(s) 790
BciVI GTATCC 1 cut(s) 1146
BclI TGATCA 5 cut(s) 559, 577, 655, 735, 1021
BcoDI GTCTC 2 cut(s) 109, 332
BcuI ACTAGT 3 cut(s) 494, 515, 1153
BfaI CTAG 5 cut(s) 111, 495, 516, 891, 1154
BfmI CTRYAG 5 cut(s) 443, 636, 663, 1002, 1029
BfuI GTATCC 1 cut(s) 1146
BisI GCNGC 9 cut(s) 97, 129, 132, 481, 541, 587, 786, 959, 978
BlsI GCNGC 9 cut(s) 98, 130, 133, 482, 542, 588, 787, 960, 979
BmcAI AGTACT 2 cut(s) 115, 1110
Bme1390I CCNGG 1 cut(s) 790
Bme18I GGWCC 2 cut(s) 551, 923
BmgT120I GGNCC 4 cut(s) 394, 551, 792, 923
BmiI GGNNCC 2 cut(s) 163, 925
BmrFI CCNGG 1 cut(s) 790
BmsI GCATC 4 cut(s) 312, 676, 1000, 1042
BpiI GAAGAC 1 cut(s) 287
BpmI CTGGAG 1 cut(s) 338
BpuEI CTTGAG 1 cut(s) 398
BsaI GGTCTC 1 cut(s) 109
BsaJI CCNNGG 3 cut(s) 405, 710, 1076
BsaWI WCCGGW 2 cut(s) 167, 253
BsaXI ACNNNNNCTCC 4 cut(s) 402, 432, 484, 514
Bsc4I CCNNNNNNNGG 4 cut(s) 100, 171, 199, 754
Bse1I ACTGG 2 cut(s) 273, 321
BseBI CCWGG 1 cut(s) 790
BseDI CCNNGG 3 cut(s) 405, 710, 1076
BseLI CCNNNNNNNGG 4 cut(s) 100, 171, 199, 754
BseNI ACTGG 2 cut(s) 273, 321
BseRI GAGGAG 2 cut(s) 172, 957
BseSI GKGCMC 1 cut(s) 261
BseXI GCAGC 8 cut(s) 83, 115, 118, 492, 552, 598, 945, 964
BseYI CCCAGC 2 cut(s) 391, 476
BsgI GTGCAG 2 cut(s) 423, 605
Bsh1285I CGRYCG 1 cut(s) 126
BshFI GGCC 5 cut(s) 396, 758, 793, 895, 1087
BsiEI CGRYCG 1 cut(s) 126
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 2 cut(s) 168, 254
BslFI GGGAC 2 cut(s) 423, 909
BslI CCNNNNNNNGG 4 cut(s) 100, 171, 199, 754
BsmAI GTCTC 2 cut(s) 109, 332
BsmBI CGTCTC 1 cut(s) 332
BsmFI GGGAC 2 cut(s) 423, 909
BsnI GGCC 5 cut(s) 396, 758, 793, 895, 1087
Bso31I GGTCTC 1 cut(s) 109
Bsp1286I GDGCHC 1 cut(s) 261
Bsp19I CCATGG 1 cut(s) 405
BspANI GGCC 5 cut(s) 396, 758, 793, 895, 1087
BspHI TCATGA 1 cut(s) 1113
BspLI GGNNCC 2 cut(s) 163, 925
BspMAI CTGCAG 1 cut(s) 447
BspPI GGATC 4 cut(s) 183, 739, 1066, 1168
BspTNI GGTCTC 1 cut(s) 109
BsrI ACTGG 2 cut(s) 273, 321
BssECI CCNNGG 3 cut(s) 405, 710, 1076
BssSI CACGAG 1 cut(s) 749
BssT1I CCWWGG 2 cut(s) 405, 1076
Bst2BI CACGAG 1 cut(s) 749
Bst2UI CCWGG 1 cut(s) 790
Bst4CI ACNGT 5 cut(s) 307, 343, 551, 733, 862
Bst6I CTCTTC 1 cut(s) 1146
BstC8I GCNNGC 4 cut(s) 6, 10, 14, 795
BstDSI CCRYGG 1 cut(s) 405
BstMAI GTCTC 2 cut(s) 109, 332
BstMCI CGRYCG 1 cut(s) 126
BstMWI GCNNNNNNNGC 4 cut(s) 128, 424, 794, 803
BstNI CCWGG 1 cut(s) 790
BstNSI RCATGY 3 cut(s) 8, 12, 16
BstSCI CCNGG 1 cut(s) 788
BstSFI CTRYAG 5 cut(s) 443, 636, 663, 1002, 1029
BstSLI GKGCMC 1 cut(s) 261
BstV1I GCAGC 8 cut(s) 83, 115, 118, 492, 552, 598, 945, 964
BstV2I GAAGAC 1 cut(s) 287
BstX2I RGATCY 3 cut(s) 175, 744, 1071
BstXI CCANNNNNNTGG 1 cut(s) 155
BstYI RGATCY 3 cut(s) 175, 744, 1071
BsuI GTATCC 1 cut(s) 1146
BsuRI GGCC 5 cut(s) 396, 758, 793, 895, 1087
BtgI CCRYGG 1 cut(s) 405
BtgZI GCGATG 2 cut(s) 582, 588
BtsI GCAGTG 1 cut(s) 440
BtsIMutI CAGTG 4 cut(s) 314, 440, 738, 858
Cac8I GCNNGC 4 cut(s) 6, 10, 14, 795
CciI TCATGA 1 cut(s) 1113
Cfr13I GGNCC 4 cut(s) 394, 551, 792, 923
Csp6I GTAC 7 cut(s) 114, 247, 266, 373, 453, 647, 1109
CviQI GTAC 7 cut(s) 114, 247, 266, 373, 453, 647, 1109
EaeI YGGCCR 2 cut(s) 756, 1085
Eam1104I CTCTTC 1 cut(s) 1146
EarI CTCTTC 1 cut(s) 1146
EciI GGCGGA 2 cut(s) 174, 492
Eco130I CCWWGG 2 cut(s) 405, 1076
Eco31I GGTCTC 1 cut(s) 109
Eco47I GGWCC 2 cut(s) 551, 923
EcoRII CCWGG 1 cut(s) 788
EcoT14I CCWWGG 2 cut(s) 405, 1076
EcoT22I ATGCAT 3 cut(s) 6, 10, 14
ErhI CCWWGG 2 cut(s) 405, 1076
Esp3I CGTCTC 1 cut(s) 332
FaqI GGGAC 2 cut(s) 423, 909
FauI CCCGC 2 cut(s) 92, 802
FauNDI CATATG 1 cut(s) 380
FbaI TGATCA 5 cut(s) 559, 577, 655, 735, 1021
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 9 cut(s) 97, 129, 132, 481, 541, 587, 786, 959, 978
Fsp4HI GCNGC 9 cut(s) 97, 129, 132, 481, 541, 587, 786, 959, 978
FspBI CTAG 5 cut(s) 111, 495, 516, 891, 1154
GluI GCNGC 9 cut(s) 97, 129, 132, 481, 541, 587, 786, 959, 978
GsaI CCCAGC 2 cut(s) 395, 480
GsuI CTGGAG 1 cut(s) 338
HaeIII GGCC 5 cut(s) 396, 758, 793, 895, 1087
HapII CCGG 2 cut(s) 168, 254
HinfI GANTC 6 cut(s) 230, 346, 411, 715, 838, 1081
HpaII CCGG 2 cut(s) 168, 254
HphI GGTGA 4 cut(s) 82, 355, 465, 1155
Hpy166II GTNNAC 4 cut(s) 249, 259, 310, 492
Hpy188I TCNGA 7 cut(s) 194, 703, 714, 774, 837, 904, 1051
Hpy188III TCNNGA 6 cut(s) 26, 554, 653, 926, 1019, 1114
Hpy8I GTNNAC 4 cut(s) 249, 259, 310, 492
Hpy99I CGWCG 1 cut(s) 851
HpyAV CCTTC 4 cut(s) 43, 600, 829, 972
HpyCH4III ACNGT 5 cut(s) 307, 343, 551, 733, 862
HpyF10VI GCNNNNNNNGC 4 cut(s) 128, 424, 794, 803
Ksp22I TGATCA 5 cut(s) 559, 577, 655, 735, 1021
LmnI GCTCC 3 cut(s) 161, 423, 811
Lsp1109I GCAGC 8 cut(s) 83, 115, 118, 492, 552, 598, 945, 964
LweI GCATC 4 cut(s) 312, 676, 1000, 1042
MaeI CTAG 5 cut(s) 111, 495, 516, 891, 1154
MaeIII GTNAC 4 cut(s) 274, 299, 727, 876
MboII GAAGA 5 cut(s) 292, 833, 1081, 1133, 1136
MflI RGATCY 3 cut(s) 175, 744, 1071
MhlI GDGCHC 1 cut(s) 261
MlsI TGGCCA 1 cut(s) 758
MluCI AATT 5 cut(s) 619, 627, 631, 985, 1058
MluNI TGGCCA 1 cut(s) 758
MlyI GAGTC 2 cut(s) 224, 405
MmeI TCCRAC 4 cut(s) 210, 552, 758, 1138
Mox20I TGGCCA 1 cut(s) 758
Mph1103I ATGCAT 3 cut(s) 6, 10, 14
MscI TGGCCA 1 cut(s) 758
MseI TTAA 4 cut(s) 462, 630, 695, 1061
MslI CAYNNNNRTG 3 cut(s) 62, 254, 330
Msp20I TGGCCA 1 cut(s) 758
MspA1I CMGCKG 3 cut(s) 96, 128, 977
MspI CCGG 2 cut(s) 168, 254
MspR9I CCNGG 1 cut(s) 790
MvaI CCWGG 1 cut(s) 790
MwoI GCNNNNNNNGC 4 cut(s) 128, 424, 794, 803
NcoI CCATGG 1 cut(s) 405
NdeI CATATG 1 cut(s) 380
NlaIV GGNNCC 2 cut(s) 163, 925
NmuCI GTSAC 2 cut(s) 274, 299
NsiI ATGCAT 3 cut(s) 6, 10, 14
NspI RCATGY 3 cut(s) 8, 12, 16
OliI CACNNNNGTG 1 cut(s) 254
PaeI GCATGC 3 cut(s) 8, 12, 16
PagI TCATGA 1 cut(s) 1113
PfeI GAWTC 4 cut(s) 346, 715, 838, 1081
PflFI GACNNNGTC 2 cut(s) 549, 921
PkrI GCNGC 9 cut(s) 98, 130, 133, 482, 542, 588, 787, 960, 979
PleI GAGTC 2 cut(s) 224, 405
PpsI GAGTC 2 cut(s) 224, 405
PshBI ATTAAT 1 cut(s) 630
Psp6I CCWGG 1 cut(s) 788
PspFI CCCAGC 2 cut(s) 391, 476
PspGI CCWGG 1 cut(s) 788
PspN4I GGNNCC 2 cut(s) 163, 925
PspPI GGNCC 4 cut(s) 394, 551, 792, 923
PstI CTGCAG 1 cut(s) 447
PsuI RGATCY 3 cut(s) 175, 744, 1071
PsyI GACNNNGTC 2 cut(s) 549, 921
PvuII CAGCTG 1 cut(s) 977
RsaI GTAC 7 cut(s) 115, 248, 267, 374, 454, 648, 1110
RsaNI GTAC 7 cut(s) 114, 247, 266, 373, 453, 647, 1109
RseI CAYNNNNRTG 3 cut(s) 62, 254, 330
SaqAI TTAA 4 cut(s) 462, 630, 695, 1061
SatI GCNGC 9 cut(s) 97, 129, 132, 481, 541, 587, 786, 959, 978
Sau96I GGNCC 4 cut(s) 394, 551, 792, 923
ScaI AGTACT 2 cut(s) 115, 1110
SchI GAGTC 2 cut(s) 224, 405
ScrFI CCNGG 1 cut(s) 790
SduI GDGCHC 1 cut(s) 261
SetI ASST 9 cut(s) 192, 248, 374, 420, 473, 652, 808, 979, 1018
SfaNI GCATC 4 cut(s) 312, 676, 1000, 1042
SfcI CTRYAG 5 cut(s) 443, 636, 663, 1002, 1029
SinI GGWCC 2 cut(s) 551, 923
SmiMI CAYNNNNRTG 3 cut(s) 62, 254, 330
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
SpeI ACTAGT 3 cut(s) 494, 515, 1153
SphI GCATGC 3 cut(s) 8, 12, 16
Sse9I AATT 5 cut(s) 619, 627, 631, 985, 1058
SspMI CTAG 5 cut(s) 111, 495, 516, 891, 1154
StyD4I CCNGG 1 cut(s) 788
StyI CCWWGG 2 cut(s) 405, 1076
TaaI ACNGT 5 cut(s) 307, 343, 551, 733, 862
TasI AATT 5 cut(s) 619, 627, 631, 985, 1058
TatI WGTACW 3 cut(s) 113, 265, 1108
TauI GCSGC 1 cut(s) 788
TfiI GAWTC 4 cut(s) 346, 715, 838, 1081
Tru1I TTAA 4 cut(s) 462, 630, 695, 1061
Tru9I TTAA 4 cut(s) 462, 630, 695, 1061
TscAI CASTG 4 cut(s) 321, 447, 738, 865
TseFI GTSAC 2 cut(s) 274, 299
TseI GCWGC 8 cut(s) 96, 128, 131, 480, 540, 586, 958, 977
Tsp45I GTSAC 2 cut(s) 274, 299
TspDTI ATGAA 4 cut(s) 62, 367, 986, 1136
TspGWI ACGGA 4 cut(s) 209, 299, 798, 960
TspRI CASTG 4 cut(s) 321, 447, 738, 865
Tth111I GACNNNGTC 2 cut(s) 549, 921
VneI GTGCAC 1 cut(s) 257
VpaK11BI GGWCC 2 cut(s) 551, 923
VspI ATTAAT 1 cut(s) 630
XceI RCATGY 3 cut(s) 8, 12, 16
XmiI GTMKAC 1 cut(s) 309
XspI CTAG 5 cut(s) 111, 495, 516, 891, 1154
ZrmI AGTACT 2 cut(s) 115, 1110
Zsp2I ATGCAT 3 cut(s) 6, 10, 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.