MD10G1063600.v1.1

fructose-bisphosphate aldolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008325
Physical Location & Seq
Reverse (-)
8651294 .. 8653908
2615 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1063600.v1.1.491

Sequence Viewer

Length: 1194 bp
ATGGCTTCTGCATCTCTCCTCAAGTCTTCTCCGGTTCTCGACAAATCTGAGTGGGTGAAGGGTCAGACCCTCCGCCAGCCTTCCGCCTCTTCGGTCGTCAGATGCCTCCCCACTGCCCCGTCTGGTCTAACCATTCGTGCCGGTTCCTATGCTGATGAGCTTGTTAAGACTGCTAAAACTGTTGCATCCCCAGGCCGTGGAATCTTGGCCATGGACGAGTCCAATGCAACTTGTGGCAAGCGTCTTGCCTCAATCGGGTTAGAGAACACTGAGGCCAACCGCCAAGCCTACCGTACCCTCCTTGTGACTGCCCCTGGCCTTGGCCAGTACGTCTCAGGTGCCATTCTCTTCGAGGAGACTCTCTACCAATCCACCGTTGATGGAAAAAAGATCGTTGATGTGCTCGTTGAGCAAGGCATCGTCCCTGGGATTAAAGTCGACAAGGGTTTGGTGCCCCTAACGGGTTCCAACGATGAGTCATGGTGCCAAGGTCTTGACGGTCTTGCCTCTCGCACAGCTGCCTACTACCAGCAGGGAGCTCGTTTCGCCAAATGGCGTACCGTTGTGAGCATCCCCAATGGCCCATCAGCTCTTGCAGTAAAGGAAGCAGCATGGGGTCTTGCCCGCTACGCTTCCATTGCTCAAGACAGCGGGTTGGTCCCAATTGTCGAGCCAGAGATCTTGCTTGATGGTGAACACACCATTGAAAGGACTTTCGAAGTGGCCTTGAAGGTCTGGGCCGAGGTTTTCTTCTACCTAGCTGAGAACAACGTCTTGTTCGAGGGTATCCTCCTCAAGCCAAGCATGGTTACTCCTGGTGCTGAGGCCAAGGAAAGAGCCACCCCTCAACAGGTTGCTGACTACACCCTCAAGCTCCTCCACAGGAGAATCCCCCCAGCTGTCCCCGGAATCATGTTTTTGTCTGGTGGACAATCTGAAGTTGAGGCAACCCTGAACTTGAATGCGATGAACCAATCACCAAACCCATGGCACGTGTCCTTCTCCTACGCCAGAGCTCTCCAAAATACCTGCCTGAAGACATGGGGAGGCAGACCGGAGAACGTGAAGGCAGCTCAGGATGCTCTGCTCATCCGTGCCAAGGCCAACTCACTTGCACAGCTGGGCAAGTACACCGGAGAGGGAGAGTCAGAGGAGGCAAAGAAGGAATTGTTTGTCAAGGGCTATGTGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00680 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00030 map00051 map00680 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
Glycolytic

Protein Analysis

398

Amino Acids

42.76

Weight (kDa)

7.6

Isoelectric Point (pI)

33.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycolytic PF00274 53 - 397 2.9e-158 Fructose-bisphosphate aldolase class-I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1037
AccB1I GGYRCC 3 cut(s) 338, 451, 483
AccB7I CCANNNNNTGG 1 cut(s) 197
AccI GTMKAC 1 cut(s) 438
AciI CCGC 5 cut(s) 73, 84, 280, 625, 651
AcoI YGGCCR 2 cut(s) 207, 322
AcuI CTGAAG 2 cut(s) 957, 1055
AcvI CACGTG 1 cut(s) 994
AfaI GTAC 5 cut(s) 295, 329, 559, 1130, 1190
AfiI CCNNNNNNNGG 7 cut(s) 197, 255, 320, 461, 708, 850, 1099
AflIII ACRYGT 1 cut(s) 993
AgsI TTSAA 3 cut(s) 707, 730, 961
AjnI CCWGG 4 cut(s) 190, 313, 424, 814
Alw21I GWGCWC 3 cut(s) 405, 541, 1018
Alw26I GTCTC 2 cut(s) 337, 350
ApeKI GCWGC 3 cut(s) 518, 608, 1070
AspS9I GGNCC 3 cut(s) 581, 658, 738
AsuC2I CCSGG 1 cut(s) 906
AsuHPI GGTGA 3 cut(s) 67, 704, 969
AsuII TTCGAA 1 cut(s) 717
AvaII GGWCC 1 cut(s) 658
BaeGI GKGCMC 1 cut(s) 456
BalI TGGCCA 2 cut(s) 209, 324
BanI GGYRCC 3 cut(s) 338, 451, 483
BanII GRGCYC 2 cut(s) 541, 1018
BbrPI CACGTG 1 cut(s) 994
BbsI GAAGAC 2 cut(s) 18, 1043
Bbv12I GWGCWC 3 cut(s) 405, 541, 1018
BbvCI CCTCAGC 1 cut(s) 822
BbvI GCAGC 3 cut(s) 505, 620, 1082
BccI CCATC 3 cut(s) 374, 592, 683
BceAI ACGGC 1 cut(s) 180
BcgI CGANNNNNNTGC 4 cut(s) 206, 240, 521, 555
BciT130I CCWGG 4 cut(s) 192, 315, 426, 816
BciVI GTATCC 1 cut(s) 797
BcnI CCSGG 1 cut(s) 906
BcoDI GTCTC 2 cut(s) 337, 350
BfaI CTAG 1 cut(s) 758
BfuAI ACCTGC 1 cut(s) 1037
BfuI GTATCC 1 cut(s) 797
BglII AGATCT 1 cut(s) 678
BisI GCNGC 3 cut(s) 519, 609, 1071
BlsI GCNGC 3 cut(s) 520, 610, 1072
Bme1390I CCNGG 5 cut(s) 192, 315, 426, 816, 906
Bme18I GGWCC 1 cut(s) 658
BmgT120I GGNCC 3 cut(s) 581, 658, 738
BmiI GGNNCC 6 cut(s) 145, 340, 453, 466, 485, 660
BmrFI CCNGG 5 cut(s) 192, 315, 426, 816, 906
BmsI GCATC 6 cut(s) 20, 92, 194, 426, 579, 1069
BpiI GAAGAC 2 cut(s) 18, 1043
Bpu10I CCTNAGC 2 cut(s) 822, 1074
Bpu14I TTCGAA 1 cut(s) 717
BpuEI CTTGAG 4 cut(s) 5, 627, 779, 854
BpuMI CCSGG 1 cut(s) 906
BsaAI YACGTR 1 cut(s) 994
BsaWI WCCGGW 3 cut(s) 31, 1054, 1133
Bsc4I CCNNNNNNNGG 7 cut(s) 197, 255, 320, 461, 708, 850, 1099
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 1 cut(s) 325
Bse3DI GCAATG 1 cut(s) 636
BseBI CCWGG 4 cut(s) 192, 315, 426, 816
BseGI GGATG 4 cut(s) 185, 570, 1084, 1089
BseLI CCNNNNNNNGG 7 cut(s) 197, 255, 320, 461, 708, 850, 1099
BseMI GCAATG 1 cut(s) 636
BseMII CTCAG 6 cut(s) 39, 261, 348, 753, 813, 1088
BseNI ACTGG 1 cut(s) 325
BseRI GAGGAG 5 cut(s) 8, 368, 782, 866, 1166
BseSI GKGCMC 1 cut(s) 456
BseXI GCAGC 3 cut(s) 505, 620, 1082
BseYI CCCAGC 2 cut(s) 895, 1120
Bsh1285I CGRYCG 1 cut(s) 96
BshNI GGYRCC 3 cut(s) 338, 451, 483
BsiEI CGRYCG 1 cut(s) 96
BsiHKAI GWGCWC 3 cut(s) 405, 541, 1018
BsiSI CCGG 5 cut(s) 32, 141, 906, 1055, 1134
BslFI GGGAC 3 cut(s) 407, 644, 887
BslI CCNNNNNNNGG 7 cut(s) 197, 255, 320, 461, 708, 850, 1099
BsmAI GTCTC 2 cut(s) 337, 350
BsmBI CGTCTC 1 cut(s) 337
BsmFI GGGAC 3 cut(s) 407, 644, 887
BsmI GAATGC 1 cut(s) 967
Bsp119I TTCGAA 1 cut(s) 717
Bsp1286I GDGCHC 4 cut(s) 405, 456, 541, 1018
Bsp143I GATC 2 cut(s) 390, 678
Bsp19I CCATGG 2 cut(s) 210, 986
BspACI CCGC 5 cut(s) 73, 84, 280, 625, 651
BspCNI CTCAG 6 cut(s) 40, 262, 347, 754, 814, 1087
BspLI GGNNCC 6 cut(s) 145, 340, 453, 466, 485, 660
BspMI ACCTGC 1 cut(s) 1037
BspT104I TTCGAA 1 cut(s) 717
BspT107I GGYRCC 3 cut(s) 338, 451, 483
BsrDI GCAATG 1 cut(s) 636
BsrFI RCCGGY 1 cut(s) 140
BsrI ACTGG 1 cut(s) 325
BssAI RCCGGY 1 cut(s) 140
BssMI GATC 2 cut(s) 390, 678
BssT1I CCWWGG 6 cut(s) 210, 319, 487, 828, 986, 1098
Bst2UI CCWGG 4 cut(s) 192, 315, 426, 816
Bst4CI ACNGT 5 cut(s) 181, 293, 376, 500, 562
Bst6I CTCTTC 2 cut(s) 94, 353
BstBAI YACGTR 1 cut(s) 994
BstBI TTCGAA 1 cut(s) 717
BstC8I GCNNGC 3 cut(s) 77, 239, 625
BstDEI CTNAG 6 cut(s) 48, 270, 334, 762, 822, 1074
BstDSI CCRYGG 3 cut(s) 196, 210, 986
BstF5I GGATG 4 cut(s) 185, 570, 1084, 1089
BstKTI GATC 2 cut(s) 393, 681
BstMAI GTCTC 2 cut(s) 337, 350
BstMBI GATC 2 cut(s) 390, 678
BstMCI CGRYCG 1 cut(s) 96
BstMWI GCNNNNNNNGC 5 cut(s) 409, 545, 629, 638, 1079
BstNI CCWGG 4 cut(s) 192, 315, 426, 816
BstSCI CCNGG 5 cut(s) 190, 313, 424, 814, 904
BstSLI GKGCMC 1 cut(s) 456
BstV1I GCAGC 3 cut(s) 505, 620, 1082
BstV2I GAAGAC 2 cut(s) 18, 1043
BstX2I RGATCY 1 cut(s) 678
BstXI CCANNNNNNTGG 1 cut(s) 987
BstYI RGATCY 1 cut(s) 678
BsuI GTATCC 1 cut(s) 797
BtgI CCRYGG 3 cut(s) 196, 210, 986
BtgZI GCGATG 1 cut(s) 980
BtsCI GGATG 4 cut(s) 185, 570, 1084, 1089
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 2 cut(s) 111, 267
BveI ACCTGC 1 cut(s) 1037
Cac8I GCNNGC 3 cut(s) 77, 239, 625
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 3 cut(s) 581, 658, 738
CseI GACGC 1 cut(s) 230
Csp6I GTAC 5 cut(s) 294, 328, 558, 1129, 1189
CviAII CATG 7 cut(s) 211, 480, 612, 805, 913, 987, 1041
CviQI GTAC 5 cut(s) 294, 328, 558, 1129, 1189
DdeI CTNAG 6 cut(s) 48, 270, 334, 762, 822, 1074
DpnI GATC 2 cut(s) 392, 680
DpnII GATC 2 cut(s) 390, 678
EaeI YGGCCR 2 cut(s) 207, 322
Eam1104I CTCTTC 2 cut(s) 94, 353
EarI CTCTTC 2 cut(s) 94, 353
EciI GGCGGA 2 cut(s) 62, 73
Ecl136II GAGCTC 2 cut(s) 539, 1016
Eco130I CCWWGG 6 cut(s) 210, 319, 487, 828, 986, 1098
Eco24I GRGCYC 2 cut(s) 541, 1018
Eco47I GGWCC 1 cut(s) 658
Eco53kI GAGCTC 2 cut(s) 539, 1016
Eco57I CTGAAG 2 cut(s) 957, 1055
Eco72I CACGTG 1 cut(s) 994
EcoICRI GAGCTC 2 cut(s) 539, 1016
EcoRII CCWGG 4 cut(s) 190, 313, 424, 814
EcoT14I CCWWGG 6 cut(s) 210, 319, 487, 828, 986, 1098
EcoT38I GRGCYC 2 cut(s) 541, 1018
ErhI CCWWGG 6 cut(s) 210, 319, 487, 828, 986, 1098
Esp3I CGTCTC 1 cut(s) 337
FaeI CATG 7 cut(s) 214, 483, 615, 808, 916, 990, 1044
FaiI YATR 9 cut(s) 150, 212, 481, 613, 806, 914, 988, 1042, 1185
FaqI GGGAC 3 cut(s) 407, 644, 887
FatI CATG 7 cut(s) 210, 479, 611, 804, 912, 986, 1040
FauI CCCGC 2 cut(s) 632, 644
FblI GTMKAC 1 cut(s) 438
Fnu4HI GCNGC 3 cut(s) 519, 609, 1071
FokI GGATG 4 cut(s) 172, 557, 1076, 1091
FriOI GRGCYC 2 cut(s) 541, 1018
Fsp4HI GCNGC 3 cut(s) 519, 609, 1071
FspBI CTAG 1 cut(s) 758
GluI GCNGC 3 cut(s) 519, 609, 1071
GsaI CCCAGC 2 cut(s) 899, 1124
HapII CCGG 5 cut(s) 32, 141, 906, 1055, 1134
HgaI GACGC 1 cut(s) 230
Hin1II CATG 7 cut(s) 214, 483, 615, 808, 916, 990, 1044
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HinfI GANTC 7 cut(s) 201, 218, 358, 476, 888, 909, 1145
HpaII CCGG 5 cut(s) 32, 141, 906, 1055, 1134
HphI GGTGA 3 cut(s) 67, 704, 969
Hpy166II GTNNAC 5 cut(s) 439, 695, 929, 1131, 1189
Hpy188I TCNGA 5 cut(s) 49, 66, 101, 937, 1150
Hpy188III TCNNGA 4 cut(s) 38, 494, 644, 1076
Hpy8I GTNNAC 5 cut(s) 439, 695, 929, 1131, 1189
HpyAV CCTTC 6 cut(s) 52, 90, 724, 1009, 1060, 1156
HpyCH4III ACNGT 5 cut(s) 181, 293, 376, 500, 562
HpyCH4IV ACGT 4 cut(s) 330, 771, 993, 1062
HpyCH4V TGCA 5 cut(s) 11, 185, 227, 596, 1115
HpyF10VI GCNNNNNNNGC 5 cut(s) 409, 545, 629, 638, 1079
HpyF3I CTNAG 6 cut(s) 48, 270, 334, 762, 822, 1074
HpySE526I ACGT 4 cut(s) 330, 771, 993, 1062
Hsp92II CATG 7 cut(s) 214, 483, 615, 808, 916, 990, 1044
Kzo9I GATC 2 cut(s) 390, 678
LmnI GCTCC 2 cut(s) 536, 879
Lsp1109I GCAGC 3 cut(s) 505, 620, 1082
LweI GCATC 6 cut(s) 20, 92, 194, 426, 579, 1069
MaeI CTAG 1 cut(s) 758
MaeII ACGT 4 cut(s) 330, 771, 993, 1062
MaeIII GTNAC 2 cut(s) 304, 808
MalI GATC 2 cut(s) 392, 680
MboI GATC 2 cut(s) 390, 678
MboII GAAGA 5 cut(s) 18, 81, 340, 742, 1048
MfeI CAATTG 1 cut(s) 663
MflI RGATCY 1 cut(s) 678
MhlI GDGCHC 4 cut(s) 405, 456, 541, 1018
MlsI TGGCCA 2 cut(s) 209, 324
MluCI AATT 2 cut(s) 663, 1166
MluNI TGGCCA 2 cut(s) 209, 324
MlyI GAGTC 4 cut(s) 227, 352, 485, 1154
MmeI TCCRAC 1 cut(s) 492
Mox20I TGGCCA 2 cut(s) 209, 324
MscI TGGCCA 2 cut(s) 209, 324
MseI TTAA 2 cut(s) 165, 432
Msp20I TGGCCA 2 cut(s) 209, 324
MspA1I CMGCKG 4 cut(s) 518, 651, 899, 1120
MspI CCGG 5 cut(s) 32, 141, 906, 1055, 1134
MspR9I CCNGG 5 cut(s) 192, 315, 426, 816, 906
MunI CAATTG 1 cut(s) 663
Mva1269I GAATGC 1 cut(s) 967
MvaI CCWGG 4 cut(s) 192, 315, 426, 816
MwoI GCNNNNNNNGC 5 cut(s) 409, 545, 629, 638, 1079
NciI CCSGG 1 cut(s) 906
NcoI CCATGG 2 cut(s) 210, 986
NdeII GATC 2 cut(s) 390, 678
NlaIII CATG 7 cut(s) 214, 483, 615, 808, 916, 990, 1044
NlaIV GGNNCC 6 cut(s) 145, 340, 453, 466, 485, 660
NmeAIII GCCGAG 1 cut(s) 766
NmuCI GTSAC 1 cut(s) 304
NspV TTCGAA 1 cut(s) 717
PasI CCCWGGG 1 cut(s) 425
PcsI WCGNNNNNNNCGW 1 cut(s) 777
PctI GAATGC 1 cut(s) 967
PfeI GAWTC 3 cut(s) 201, 888, 909
PflMI CCANNNNNTGG 1 cut(s) 197
PkrI GCNGC 3 cut(s) 520, 610, 1072
PleI GAGTC 4 cut(s) 226, 352, 484, 1153
PmaCI CACGTG 1 cut(s) 994
PmlI CACGTG 1 cut(s) 994
PpsI GAGTC 4 cut(s) 226, 352, 484, 1153
Ppu21I YACGTR 1 cut(s) 994
Psp124BI GAGCTC 2 cut(s) 541, 1018
Psp6I CCWGG 4 cut(s) 190, 313, 424, 814
PspCI CACGTG 1 cut(s) 994
PspFI CCCAGC 2 cut(s) 895, 1120
PspGI CCWGG 4 cut(s) 190, 313, 424, 814
PspN4I GGNNCC 6 cut(s) 145, 340, 453, 466, 485, 660
PspPI GGNCC 3 cut(s) 581, 658, 738
PsuI RGATCY 1 cut(s) 678
PvuII CAGCTG 3 cut(s) 518, 899, 1120
RsaI GTAC 5 cut(s) 295, 329, 559, 1130, 1190
RsaNI GTAC 5 cut(s) 294, 328, 558, 1129, 1189
SacI GAGCTC 2 cut(s) 541, 1018
SalI GTCGAC 1 cut(s) 437
SaqAI TTAA 2 cut(s) 165, 432
SatI GCNGC 3 cut(s) 519, 609, 1071
Sau3AI GATC 2 cut(s) 390, 678
Sau96I GGNCC 3 cut(s) 581, 658, 738
SchI GAGTC 4 cut(s) 227, 352, 485, 1154
ScrFI CCNGG 5 cut(s) 192, 315, 426, 816, 906
SduI GDGCHC 4 cut(s) 405, 456, 541, 1018
SfaNI GCATC 6 cut(s) 20, 92, 194, 426, 579, 1069
SfuI TTCGAA 1 cut(s) 717
SinI GGWCC 1 cut(s) 658
SmlI CTYRAG 4 cut(s) 20, 642, 794, 869
SmoI CTYRAG 4 cut(s) 20, 642, 794, 869
Sse9I AATT 2 cut(s) 663, 1166
SsiI CCGC 5 cut(s) 73, 84, 280, 625, 651
SspMI CTAG 1 cut(s) 758
SstI GAGCTC 2 cut(s) 541, 1018
StyD4I CCNGG 5 cut(s) 190, 313, 424, 814, 904
StyI CCWWGG 6 cut(s) 210, 319, 487, 828, 986, 1098
TaaI ACNGT 5 cut(s) 181, 293, 376, 500, 562
TaiI ACGT 4 cut(s) 333, 774, 996, 1065
TaqI TCGA 6 cut(s) 39, 351, 438, 669, 717, 780
TaqII GACCGA 1 cut(s) 82
TasI AATT 2 cut(s) 663, 1166
TatI WGTACW 2 cut(s) 1128, 1188
TfiI GAWTC 3 cut(s) 201, 888, 909
Tru1I TTAA 2 cut(s) 165, 432
Tru9I TTAA 2 cut(s) 165, 432
TscAI CASTG 2 cut(s) 118, 274
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 3 cut(s) 518, 608, 1070
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 1 cut(s) 983
TspGWI ACGGA 1 cut(s) 1082
TspRI CASTG 2 cut(s) 118, 274
Van91I CCANNNNNTGG 1 cut(s) 197
VpaK11BI GGWCC 1 cut(s) 658
XmiI GTMKAC 1 cut(s) 438
XspI CTAG 1 cut(s) 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.