Rw6G013440

fructose-bisphosphate aldolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0008325
Physical Location & Seq
Forward (+)
24818781 .. 24820795
2015 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G013440.1

Sequence Viewer

Length: 1149 bp
ATGGCATCTGCGTCTCTACTCAAGTCTTCTCCAGTTCTTGACAAATCTGAGTGGGTGAAAGGCCAGACCCTCCTCCGCCAGCCTTCTGTCTCATCCATCGTCAGATGCCACCCTACCGCCACATCTGGTCTTACCATTCGTGCTTCATATGCTGATGAACTTGTCAAGACCGCTAAAACTGTTGCATCCCCCGGCCGTGGAATTTTGGCCATGGATGAATCCAATGCAACCTGTGGCAAGCGTCTGGCTTCAATTGGGCTAGAGAACACTGAGGCTAACCGCCAAGCCTACCGTACCCTCCTTGTGTCAGTCCCTGGCCTTGGCAATTACGTCTCTGGTGCCATTCTTTTCGAGGAAACTCTCTACCAATCCACTGTTGATGGTAAGAAGATTGTGGATGTGCTCGTTGAGCAAGGCATCGTCCCTGGGATCAAAGTTGACAAGGGTTTGGTGCCCCTCGCTGGTTCCAACAATGAGTCATGGTGCCAAGGTCTTGATGGACTTGCTTCTCGCACAGCTGCTTACTACCAACAGGGAGCTCGTTTCGCCAAATGGCGTACTGTTGTGAGCATTCCCAATGGTCCATCCGCTCTTGCAGTAAAGGAAGCAGCCTGGGGTCTCGCTCGCTACGCTGCCATTTCTCAAGAAAGTGGATTGGTCCCAATCGTAGAGCCAGAGATCTTGCTTGATGGTGAGCACGGCATTGACAGGACCTTCGAAGTTGCTAAAGCAGTGTGGGCTGAGGTTTTCTTCTACCTTGCCCAGAACAATGTCTTGTTTGAGGGTATCCTCCTCAAGCCTAGTATGGTTACTCCTGGAGCTGAGTGCAAGGAAAGAGCCACCCCGGAACAGGTTGCCGACTACACCCTCAAGCTCCTCCACAGGAGAATCCCCCCAGCAGTCCCTGGAATCATGTTTTTGTCTGGTGGACAATCTGAGGTTGAGGCAACCCTGAACTTGAATGCAATGAACCAATCGCCAAACCCGTGGCATGTGTCATTCTCCTATGCCAGAGCTCTCCAGAACACTTGCCTGAAGACATGGGGAGGCCGACCAGAGAACGTGAAGGCCGCTCAGGAGGCACTACTTATCCAAGGAGAATCAGAGGAGGCAAAGAAAGAATTGTTCGTCAAGGGCTACGTGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00680 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00030 map00051 map00680 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
Glycolytic

Protein Analysis

382

Amino Acids

41.25

Weight (kDa)

6.92

Isoelectric Point (pI)

35.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycolytic PF00274 53 - 367 1.4e-146 Fructose-bisphosphate aldolase class-I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 338, 451, 483
AccBSI CCGCTC 2 cut(s) 590, 1073
AciI CCGC 6 cut(s) 76, 117, 171, 280, 588, 1071
AclWI GGATC 1 cut(s) 437
AcoI YGGCCR 2 cut(s) 193, 207
AcsI RAATTY 1 cut(s) 201
AcuI CTGAAG 1 cut(s) 1055
AfaI GTAC 3 cut(s) 295, 559, 1145
AfiI CCNNNNNNNGG 4 cut(s) 197, 320, 461, 850
AflIII ACRYGT 1 cut(s) 1140
AgsI TTSAA 2 cut(s) 252, 961
AjnI CCWGG 5 cut(s) 313, 424, 611, 814, 904
AluBI AGCT 5 cut(s) 518, 539, 821, 874, 1016
AluI AGCT 5 cut(s) 518, 539, 821, 874, 1016
Alw21I GWGCWC 4 cut(s) 405, 541, 699, 1018
Alw26I GTCTC 4 cut(s) 18, 94, 337, 623
AlwI GGATC 1 cut(s) 437
AlwNI CAGNNNCTG 2 cut(s) 314, 905
AoxI GGCC 6 cut(s) 61, 193, 207, 316, 1048, 1068
ApeKI GCWGC 3 cut(s) 518, 608, 632
ApoI RAATTY 1 cut(s) 201
ArsI GACNNNNNNTTYG 2 cut(s) 698, 730
AspS9I GGNCC 3 cut(s) 581, 658, 711
AsuC2I CCSGG 2 cut(s) 192, 845
AsuHPI GGTGA 2 cut(s) 67, 704
AsuII TTCGAA 1 cut(s) 717
AvaII GGWCC 3 cut(s) 581, 658, 711
BaeGI GKGCMC 1 cut(s) 456
BalI TGGCCA 1 cut(s) 209
BanI GGYRCC 3 cut(s) 338, 451, 483
BanII GRGCYC 2 cut(s) 541, 1018
BbsI GAAGAC 2 cut(s) 18, 1043
Bbv12I GWGCWC 4 cut(s) 405, 541, 699, 1018
BbvCI CCTCAGC 1 cut(s) 741
BbvI GCAGC 3 cut(s) 505, 619, 620
BccI CCATC 5 cut(s) 104, 374, 491, 592, 683
BceAI ACGGC 2 cut(s) 180, 715
BciT130I CCWGG 5 cut(s) 315, 426, 613, 816, 906
BciVI GTATCC 1 cut(s) 797
BcnI CCSGG 2 cut(s) 192, 845
BcoDI GTCTC 4 cut(s) 18, 94, 337, 623
BfaI CTAG 3 cut(s) 260, 801, 1147
BfuI GTATCC 1 cut(s) 797
BglII AGATCT 1 cut(s) 678
BisI GCNGC 4 cut(s) 519, 609, 633, 1071
BlsI GCNGC 4 cut(s) 520, 610, 634, 1072
Bme1390I CCNGG 7 cut(s) 192, 315, 426, 613, 816, 845, 906
Bme18I GGWCC 3 cut(s) 581, 658, 711
BmgT120I GGNCC 3 cut(s) 581, 658, 711
BmiI GGNNCC 5 cut(s) 340, 453, 466, 485, 660
BmrFI CCNGG 7 cut(s) 192, 315, 426, 613, 816, 845, 906
BmsI GCATC 4 cut(s) 14, 95, 194, 426
BpiI GAAGAC 2 cut(s) 18, 1043
BpmI CTGGAG 3 cut(s) 15, 837, 1004
Bpu10I CCTNAGC 2 cut(s) 741, 1074
Bpu14I TTCGAA 1 cut(s) 717
BpuEI CTTGAG 4 cut(s) 5, 627, 779, 854
BpuMI CCSGG 2 cut(s) 192, 845
BsaAI YACGTR 1 cut(s) 1141
BsaI GGTCTC 1 cut(s) 623
Bsc4I CCNNNNNNNGG 4 cut(s) 197, 320, 461, 850
Bse1I ACTGG 1 cut(s) 32
Bse3DI GCAATG 1 cut(s) 972
BseBI CCWGG 5 cut(s) 315, 426, 613, 816, 906
BseGI GGATG 5 cut(s) 92, 185, 220, 403, 584
BseLI CCNNNNNNNGG 4 cut(s) 197, 320, 461, 850
BseMI GCAATG 1 cut(s) 972
BseMII CTCAG 6 cut(s) 39, 261, 732, 813, 927, 1088
BseNI ACTGG 1 cut(s) 32
BseRI GAGGAG 4 cut(s) 62, 782, 866, 1121
BseSI GKGCMC 1 cut(s) 456
BseX3I CGGCCG 1 cut(s) 193
BseXI GCAGC 3 cut(s) 505, 619, 620
BseYI CCCAGC 1 cut(s) 895
Bsh1285I CGRYCG 1 cut(s) 196
BshFI GGCC 6 cut(s) 63, 195, 209, 318, 1050, 1070
BshNI GGYRCC 3 cut(s) 338, 451, 483
BsiEI CGRYCG 1 cut(s) 196
BsiHKAI GWGCWC 4 cut(s) 405, 541, 699, 1018
BsiSI CCGG 2 cut(s) 192, 845
BslFI GGGAC 4 cut(s) 296, 407, 644, 887
BslI CCNNNNNNNGG 4 cut(s) 197, 320, 461, 850
BsmAI GTCTC 4 cut(s) 18, 94, 337, 623
BsmBI CGTCTC 2 cut(s) 18, 337
BsmFI GGGAC 4 cut(s) 296, 407, 644, 887
BsmI GAATGC 2 cut(s) 570, 967
BsnI GGCC 6 cut(s) 63, 195, 209, 318, 1050, 1070
Bso31I GGTCTC 1 cut(s) 623
Bsp119I TTCGAA 1 cut(s) 717
Bsp1286I GDGCHC 5 cut(s) 405, 456, 541, 699, 1018
Bsp143I GATC 2 cut(s) 429, 678
Bsp19I CCATGG 1 cut(s) 210
BspACI CCGC 6 cut(s) 76, 117, 171, 280, 588, 1071
BspANI GGCC 6 cut(s) 63, 195, 209, 318, 1050, 1070
BspCNI CTCAG 6 cut(s) 40, 262, 733, 814, 928, 1087
BspLI GGNNCC 5 cut(s) 340, 453, 466, 485, 660
BspPI GGATC 1 cut(s) 437
BspT104I TTCGAA 1 cut(s) 717
BspT107I GGYRCC 3 cut(s) 338, 451, 483
BspTNI GGTCTC 1 cut(s) 623
BsrBI CCGCTC 2 cut(s) 590, 1073
BsrDI GCAATG 1 cut(s) 972
BsrI ACTGG 1 cut(s) 32
BssMI GATC 2 cut(s) 429, 678
BssT1I CCWWGG 4 cut(s) 210, 319, 487, 1093
Bst2UI CCWGG 5 cut(s) 315, 426, 613, 816, 906
Bst4CI ACNGT 4 cut(s) 181, 293, 376, 562
BstBAI YACGTR 1 cut(s) 1141
BstBI TTCGAA 1 cut(s) 717
BstC8I GCNNGC 3 cut(s) 80, 239, 625
BstDEI CTNAG 6 cut(s) 48, 270, 741, 822, 936, 1074
BstDSI CCRYGG 3 cut(s) 196, 210, 986
BstF5I GGATG 5 cut(s) 92, 185, 220, 403, 584
BstKTI GATC 2 cut(s) 432, 681
BstMAI GTCTC 4 cut(s) 18, 94, 337, 623
BstMBI GATC 2 cut(s) 429, 678
BstMCI CGRYCG 1 cut(s) 196
BstMWI GCNNNNNNNGC 6 cut(s) 149, 409, 545, 629, 737, 1079
BstNI CCWGG 5 cut(s) 315, 426, 613, 816, 906
BstNSI RCATGY 1 cut(s) 995
BstSCI CCNGG 7 cut(s) 190, 313, 424, 611, 814, 843, 904
BstSLI GKGCMC 1 cut(s) 456
BstV1I GCAGC 3 cut(s) 505, 619, 620
BstV2I GAAGAC 2 cut(s) 18, 1043
BstX2I RGATCY 1 cut(s) 678
BstXI CCANNNNNNTGG 1 cut(s) 987
BstYI RGATCY 1 cut(s) 678
BstZI CGGCCG 1 cut(s) 193
BsuI GTATCC 1 cut(s) 797
BsuRI GGCC 6 cut(s) 63, 195, 209, 318, 1050, 1070
BtgI CCRYGG 3 cut(s) 196, 210, 986
BtsCI GGATG 5 cut(s) 92, 185, 220, 403, 584
BtsI GCAGTG 1 cut(s) 738
BtsIMutI CAGTG 3 cut(s) 267, 372, 738
Cac8I GCNNGC 3 cut(s) 80, 239, 625
CaiI CAGNNNCTG 2 cut(s) 314, 905
Cfr13I GGNCC 3 cut(s) 581, 658, 711
CseI GACGC 1 cut(s) 230
Csp6I GTAC 3 cut(s) 294, 558, 1144
CviAII CATG 5 cut(s) 211, 480, 913, 992, 1041
CviQI GTAC 3 cut(s) 294, 558, 1144
DdeI CTNAG 6 cut(s) 48, 270, 741, 822, 936, 1074
DpnI GATC 2 cut(s) 431, 680
DpnII GATC 2 cut(s) 429, 678
EaeI YGGCCR 2 cut(s) 193, 207
EagI CGGCCG 1 cut(s) 193
EciI GGCGGA 1 cut(s) 65
Ecl136II GAGCTC 2 cut(s) 539, 1016
EclXI CGGCCG 1 cut(s) 193
Eco130I CCWWGG 4 cut(s) 210, 319, 487, 1093
Eco24I GRGCYC 2 cut(s) 541, 1018
Eco31I GGTCTC 1 cut(s) 623
Eco47I GGWCC 3 cut(s) 581, 658, 711
Eco52I CGGCCG 1 cut(s) 193
Eco53kI GAGCTC 2 cut(s) 539, 1016
Eco57I CTGAAG 1 cut(s) 1055
EcoICRI GAGCTC 2 cut(s) 539, 1016
EcoO109I RGGNCCY 1 cut(s) 711
EcoRII CCWGG 5 cut(s) 313, 424, 611, 814, 904
EcoT14I CCWWGG 4 cut(s) 210, 319, 487, 1093
EcoT38I GRGCYC 2 cut(s) 541, 1018
ErhI CCWWGG 4 cut(s) 210, 319, 487, 1093
Esp3I CGTCTC 2 cut(s) 18, 337
FaeI CATG 5 cut(s) 214, 483, 916, 995, 1044
FaiI YATR 9 cut(s) 148, 150, 212, 481, 806, 914, 993, 1008, 1042
FaqI GGGAC 4 cut(s) 296, 407, 644, 887
FatI CATG 5 cut(s) 210, 479, 912, 991, 1040
FauNDI CATATG 1 cut(s) 148
Fnu4HI GCNGC 4 cut(s) 519, 609, 633, 1071
FokI GGATG 5 cut(s) 79, 172, 227, 410, 571
FriOI GRGCYC 2 cut(s) 541, 1018
Fsp4HI GCNGC 4 cut(s) 519, 609, 633, 1071
FspBI CTAG 3 cut(s) 260, 801, 1147
GluI GCNGC 4 cut(s) 519, 609, 633, 1071
GsaI CCCAGC 1 cut(s) 899
GsuI CTGGAG 3 cut(s) 15, 837, 1004
HaeIII GGCC 6 cut(s) 63, 195, 209, 318, 1050, 1070
HapII CCGG 2 cut(s) 192, 845
HgaI GACGC 1 cut(s) 230
Hin1II CATG 5 cut(s) 214, 483, 916, 995, 1044
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HinfI GANTC 5 cut(s) 218, 476, 888, 909, 1100
HpaII CCGG 2 cut(s) 192, 845
HphI GGTGA 2 cut(s) 67, 704
Hpy166II GTNNAC 3 cut(s) 439, 929, 1144
Hpy188I TCNGA 4 cut(s) 49, 104, 937, 1105
Hpy188III TCNNGA 6 cut(s) 38, 166, 494, 644, 1021, 1076
Hpy8I GTNNAC 3 cut(s) 439, 929, 1144
HpyAV CCTTC 3 cut(s) 93, 724, 1060
HpyCH4III ACNGT 4 cut(s) 181, 293, 376, 562
HpyCH4IV ACGT 3 cut(s) 330, 1062, 1140
HpyCH4V TGCA 5 cut(s) 185, 227, 596, 828, 965
HpyF10VI GCNNNNNNNGC 6 cut(s) 149, 409, 545, 629, 737, 1079
HpyF3I CTNAG 6 cut(s) 48, 270, 741, 822, 936, 1074
HpySE526I ACGT 3 cut(s) 330, 1062, 1140
Hsp92II CATG 5 cut(s) 214, 483, 916, 995, 1044
Kzo9I GATC 2 cut(s) 429, 678
LmnI GCTCC 3 cut(s) 536, 818, 879
Lsp1109I GCAGC 3 cut(s) 505, 619, 620
LweI GCATC 4 cut(s) 14, 95, 194, 426
MaeI CTAG 3 cut(s) 260, 801, 1147
MaeII ACGT 3 cut(s) 330, 1062, 1140
MaeIII GTNAC 1 cut(s) 808
MalI GATC 2 cut(s) 431, 680
MbiI CCGCTC 2 cut(s) 590, 1073
MboI GATC 2 cut(s) 429, 678
MboII GAAGA 4 cut(s) 18, 400, 742, 1048
MfeI CAATTG 1 cut(s) 252
MflI RGATCY 1 cut(s) 678
MhlI GDGCHC 5 cut(s) 405, 456, 541, 699, 1018
MlsI TGGCCA 1 cut(s) 209
MluCI AATT 4 cut(s) 201, 252, 325, 1121
MluNI TGGCCA 1 cut(s) 209
MlyI GAGTC 1 cut(s) 485
MmeI TCCRAC 1 cut(s) 492
Mox20I TGGCCA 1 cut(s) 209
MscI TGGCCA 1 cut(s) 209
Msp20I TGGCCA 1 cut(s) 209
MspA1I CMGCKG 1 cut(s) 518
MspI CCGG 2 cut(s) 192, 845
MspR9I CCNGG 7 cut(s) 192, 315, 426, 613, 816, 845, 906
MunI CAATTG 1 cut(s) 252
Mva1269I GAATGC 2 cut(s) 570, 967
MvaI CCWGG 5 cut(s) 315, 426, 613, 816, 906
MwoI GCNNNNNNNGC 6 cut(s) 149, 409, 545, 629, 737, 1079
NciI CCSGG 2 cut(s) 192, 845
NcoI CCATGG 1 cut(s) 210
NdeI CATATG 1 cut(s) 148
NdeII GATC 2 cut(s) 429, 678
NlaIII CATG 5 cut(s) 214, 483, 916, 995, 1044
NlaIV GGNNCC 5 cut(s) 340, 453, 466, 485, 660
NspI RCATGY 1 cut(s) 995
NspV TTCGAA 1 cut(s) 717
PasI CCCWGGG 1 cut(s) 425
PcsI WCGNNNNNNNCGW 1 cut(s) 983
PctI GAATGC 2 cut(s) 570, 967
PfeI GAWTC 4 cut(s) 218, 888, 909, 1100
PfoI TCCNGGA 1 cut(s) 814
PkrI GCNGC 4 cut(s) 520, 610, 634, 1072
PleI GAGTC 1 cut(s) 484
PpsI GAGTC 1 cut(s) 484
Ppu21I YACGTR 1 cut(s) 1141
PpuMI RGGWCCY 1 cut(s) 711
Psp124BI GAGCTC 2 cut(s) 541, 1018
Psp5II RGGWCCY 1 cut(s) 711
Psp6I CCWGG 5 cut(s) 313, 424, 611, 814, 904
PspFI CCCAGC 1 cut(s) 895
PspGI CCWGG 5 cut(s) 313, 424, 611, 814, 904
PspN4I GGNNCC 5 cut(s) 340, 453, 466, 485, 660
PspPI GGNCC 3 cut(s) 581, 658, 711
PspPPI RGGWCCY 1 cut(s) 711
PstNI CAGNNNCTG 2 cut(s) 314, 905
PsuI RGATCY 1 cut(s) 678
PvuII CAGCTG 1 cut(s) 518
RsaI GTAC 3 cut(s) 295, 559, 1145
RsaNI GTAC 3 cut(s) 294, 558, 1144
SacI GAGCTC 2 cut(s) 541, 1018
SatI GCNGC 4 cut(s) 519, 609, 633, 1071
Sau3AI GATC 2 cut(s) 429, 678
Sau96I GGNCC 3 cut(s) 581, 658, 711
SchI GAGTC 1 cut(s) 485
ScrFI CCNGG 7 cut(s) 192, 315, 426, 613, 816, 845, 906
SduI GDGCHC 5 cut(s) 405, 456, 541, 699, 1018
SfaNI GCATC 4 cut(s) 14, 95, 194, 426
SfuI TTCGAA 1 cut(s) 717
SinI GGWCC 3 cut(s) 581, 658, 711
SmlI CTYRAG 4 cut(s) 20, 642, 794, 869
SmoI CTYRAG 4 cut(s) 20, 642, 794, 869
Sse9I AATT 4 cut(s) 201, 252, 325, 1121
SsiI CCGC 6 cut(s) 76, 117, 171, 280, 588, 1071
SspMI CTAG 3 cut(s) 260, 801, 1147
SstI GAGCTC 2 cut(s) 541, 1018
StyD4I CCNGG 7 cut(s) 190, 313, 424, 611, 814, 843, 904
StyI CCWWGG 4 cut(s) 210, 319, 487, 1093
TaaI ACNGT 4 cut(s) 181, 293, 376, 562
TaiI ACGT 3 cut(s) 333, 1065, 1143
TaqI TCGA 2 cut(s) 351, 717
TasI AATT 4 cut(s) 201, 252, 325, 1121
TatI WGTACW 1 cut(s) 1143
TauI GCSGC 1 cut(s) 1073
TfiI GAWTC 4 cut(s) 218, 888, 909, 1100
TscAI CASTG 3 cut(s) 274, 379, 738
TseI GCWGC 3 cut(s) 518, 608, 632
TspDTI ATGAA 4 cut(s) 135, 171, 231, 983
TspRI CASTG 3 cut(s) 274, 379, 738
VpaK11BI GGWCC 3 cut(s) 581, 658, 711
XapI RAATTY 1 cut(s) 201
XceI RCATGY 1 cut(s) 995
XcmI CCANNNNNNNNNTGG 1 cut(s) 494
XspI CTAG 3 cut(s) 260, 801, 1147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.