MD10G1213600.v1.1

Pentatricopeptide repeat-containing protein At1g79490

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0024228
Physical Location & Seq
Reverse (-)
31216760 .. 31218206
1447 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1213600.v1.1.491

Sequence Viewer

Length: 1254 bp
ATGGTAGGCGGGTTGAAAAAGCCGATGTTGAATGCCTCTGCCATTGCTAAGATTGTTGAGGTTGTGAAGAGGTGGAAATGGGGGCCTGAATTGGAGACCCAATTAGACAAACTCCAATTTGTGCCAAACATGACTCACATTACTCAAGCCTTGAAGGTTATTAAGGATGGCGATGGTGCTTTGAGTTTGTTTCGATGGGCTAAGAGGCAGGCGTGGTATTTGCCGAGTGACGAGTGTTATGTGGTGTTGTTTGATGGCCTAAACCAAAGTAAGGACTTCAATGGAATCCAATCCTTGTTTGATGAAATGGTGCAGGATTCAGGTAGTAGTGGGGTTTTGTCATCTGGTGCGTATAATCGCGTCATTCAGTTCTTGGCTAAAGCAGATAAAATGGAGGTGGCATTTTGTTGCTTTAAGAAGATTCAAGATTCGGGTTTTGAAGTTGATACTCAGACATGTAATTCACTTATATTGCTGTTTCTAAATAAGGGTTTACCTTATAAGGCATTTGAGGTATATGAGAGCATGCAAGCTGCAAGCTTTTCATTAGATGGATCAACCTATGAGTTGATGGGCTACGGTTCTATGATTGAGTCATATGTAAGGGCTGGCAAATTAGATGCTGCCCTTAGGCTTTGGGATGAGATGAAGAGAGTGGACTTTAGGCCTAACTTTGGGTTGTACACAATGATTGTTGAGTCCCATGCAAAATCAGGGAAACTTGATACTGCAATGTCTACCTTTACCGATATGGAGAAGGCTGGATTTCTACCCACTCCTTCCACATATTCGTGTCTATTGGAAATGCATGCTGCCTCTGGACAAGTAGATTCTGCTATGAAGCTGTATAACTCAATGACCAATGCAGGTTTAAGACCAGGTCTAAGTACTTACACATCTCTTTTGATGCTTTTGGCTAATAAGAAGCTTGTGGACGTGGCTGCCAAGATTTTGCTTGAGATGAAGGCCATGGGTTATTCTGTTGATGTGAGCGCTAGTGATGTTTTGATGGTGTATATCAAGGAGGGTTCTGTTGATGTAGCCTTGCGGTGGTTGCGCTTCATGGGCTCATCAGGGATTAGAACAAATAATTTTATAATCAGACAGTTGTTCGAGTCATGTATGAAGAGTGGTTTGAATGAGTCAGCGAAGCCTCTCTTAGAAATGTATGTGAATACTTGGTGCAAAAGTCGACCTCATACTTTACACATCCATTCTAGCCTATCTCGTAAGATGCCAGGAAGAAGAGCATGA

Protein Analysis

418

Amino Acids

46.59

Weight (kDa)

8.76

Isoelectric Point (pI)

36.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 116 - 157 2.5e-06 PPR repeat family
PPR_2 PF13041 192 - 224 2.5e-06 PPR repeat family
PPR PF01535 193 - 218 3.2e-06 PPR repeat
PPR_long PF17177 197 - 329 3.6e-11 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 211 - 269 3.7e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 224 - 269 1.2e-07 PPR repeat family
TPR_24 PF23276 229 - 336 2.6e-09 Fungal tetratrico peptide repeats
PPR_2 PF13041 262 - 304 1.3e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0024228)

Species Orthologous Gene IDs
malus_domestica MD10G1213600.v1.1
pyrus_communis pycom10g18030
rosa_multiflora Rmu_co8174666.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 501, 1097
Acc36I ACCTGC 1 cut(s) 857
AccI GTMKAC 2 cut(s) 737, 1192
AccII CGCG 1 cut(s) 360
AciI CCGC 2 cut(s) 9, 1048
AclWI GGATC 1 cut(s) 562
AfaI GTAC 2 cut(s) 683, 889
AfeI AGCGCT 1 cut(s) 994
AfiI CCNNNNNNNGG 3 cut(s) 271, 674, 1050
AflIII ACRYGT 1 cut(s) 455
AgsI TTSAA 7 cut(s) 16, 31, 154, 280, 425, 440, 1138
AjiI CACGTC 1 cut(s) 937
AjnI CCWGG 2 cut(s) 877, 1237
AluBI AGCT 4 cut(s) 533, 540, 844, 928
AluI AGCT 4 cut(s) 533, 540, 844, 928
Alw26I GTCTC 1 cut(s) 89
AlwI GGATC 1 cut(s) 562
Aor51HI AGCGCT 1 cut(s) 994
AoxI GGCC 4 cut(s) 83, 256, 665, 966
ApeKI GCWGC 4 cut(s) 533, 623, 812, 941
AspLEI GCGC 2 cut(s) 995, 1059
AspS9I GGNCC 1 cut(s) 83
AxyI CCTNAGG 1 cut(s) 629
BanII GRGCYC 1 cut(s) 1070
BbvI GCAGC 4 cut(s) 520, 610, 799, 928
BccI CCATC 7 cut(s) 161, 167, 189, 248, 545, 565, 1003
BciT130I CCWGG 2 cut(s) 879, 1239
BcoDI GTCTC 1 cut(s) 89
BfaI CTAG 2 cut(s) 996, 1218
BfoI RGCGCY 1 cut(s) 996
BfuAI ACCTGC 1 cut(s) 857
BisI GCNGC 4 cut(s) 534, 624, 813, 942
BlsI GCNGC 4 cut(s) 535, 625, 814, 943
BmcAI AGTACT 1 cut(s) 889
Bme1390I CCNGG 2 cut(s) 879, 1239
BmgBI CACGTC 1 cut(s) 937
BmgT120I GGNCC 1 cut(s) 83
BmiI GGNNCC 1 cut(s) 84
BmrFI CCNGG 2 cut(s) 879, 1239
BmsI GCATC 3 cut(s) 610, 897, 1224
BpuEI CTTGAG 2 cut(s) 129, 977
BsaI GGTCTC 1 cut(s) 89
BsaJI CCNNGG 1 cut(s) 969
Bsc4I CCNNNNNNNGG 3 cut(s) 271, 674, 1050
Bse21I CCTNAGG 1 cut(s) 629
Bse3DI GCAATG 2 cut(s) 42, 738
BseBI CCWGG 2 cut(s) 879, 1239
BseDI CCNNGG 1 cut(s) 969
BseGI GGATG 3 cut(s) 172, 646, 1209
BseLI CCNNNNNNNGG 3 cut(s) 271, 674, 1050
BseMI GCAATG 2 cut(s) 42, 738
BseMII CTCAG 1 cut(s) 464
BseXI GCAGC 4 cut(s) 520, 610, 799, 928
BsgI GTGCAG 1 cut(s) 332
Bsh1236I CGCG 1 cut(s) 360
BshFI GGCC 4 cut(s) 85, 258, 667, 968
BslFI GGGAC 1 cut(s) 685
BslI CCNNNNNNNGG 3 cut(s) 271, 674, 1050
BsmAI GTCTC 1 cut(s) 89
BsmFI GGGAC 1 cut(s) 685
BsmI GAATGC 1 cut(s) 37
BsnI GGCC 4 cut(s) 85, 258, 667, 968
Bso31I GGTCTC 1 cut(s) 89
Bsp1286I GDGCHC 1 cut(s) 1070
Bsp1407I TGTACA 1 cut(s) 681
Bsp143I GATC 1 cut(s) 554
Bsp19I CCATGG 1 cut(s) 969
BspACI CCGC 2 cut(s) 9, 1048
BspANI GGCC 4 cut(s) 85, 258, 667, 968
BspCNI CTCAG 1 cut(s) 463
BspFNI CGCG 1 cut(s) 360
BspLI GGNNCC 1 cut(s) 84
BspMI ACCTGC 1 cut(s) 857
BspPI GGATC 1 cut(s) 562
BspQI GCTCTTC 1 cut(s) 1240
BspTNI GGTCTC 1 cut(s) 89
BsrDI GCAATG 2 cut(s) 42, 738
BsrGI TGTACA 1 cut(s) 681
BssECI CCNNGG 1 cut(s) 969
BssMI GATC 1 cut(s) 554
BssT1I CCWWGG 1 cut(s) 969
Bst2UI CCWGG 2 cut(s) 879, 1239
Bst4CI ACNGT 2 cut(s) 581, 1107
Bst6I CTCTTC 4 cut(s) 62, 644, 1121, 1240
BstAUI TGTACA 1 cut(s) 681
BstC8I GCNNGC 6 cut(s) 210, 527, 531, 538, 610, 810
BstDEI CTNAG 6 cut(s) 48, 201, 450, 629, 884, 1159
BstDSI CCRYGG 1 cut(s) 969
BstF5I GGATG 3 cut(s) 172, 646, 1209
BstFNI CGCG 1 cut(s) 360
BstH2I RGCGCY 1 cut(s) 996
BstHHI GCGC 2 cut(s) 995, 1059
BstKTI GATC 1 cut(s) 557
BstMAI GTCTC 1 cut(s) 89
BstMBI GATC 1 cut(s) 554
BstMWI GCNNNNNNNGC 2 cut(s) 1054, 1065
BstNI CCWGG 2 cut(s) 879, 1239
BstNSI RCATGY 3 cut(s) 459, 529, 812
BstSCI CCNGG 2 cut(s) 877, 1237
BstUI CGCG 1 cut(s) 360
BstV1I GCAGC 4 cut(s) 520, 610, 799, 928
Bsu36I CCTNAGG 1 cut(s) 629
BsuRI GGCC 4 cut(s) 85, 258, 667, 968
BtgI CCRYGG 1 cut(s) 969
BtgZI GCGATG 1 cut(s) 186
BtrI CACGTC 1 cut(s) 937
BtsCI GGATG 3 cut(s) 172, 646, 1209
BveI ACCTGC 1 cut(s) 857
Cac8I GCNNGC 6 cut(s) 210, 527, 531, 538, 610, 810
CfoI GCGC 2 cut(s) 995, 1059
Cfr13I GGNCC 1 cut(s) 83
CseI GACGC 1 cut(s) 349
CsiI ACCWGGT 1 cut(s) 877
Csp6I GTAC 2 cut(s) 682, 888
CviAII CATG 9 cut(s) 130, 456, 526, 704, 809, 970, 1063, 1119, 1251
CviQI GTAC 2 cut(s) 682, 888
DdeI CTNAG 6 cut(s) 48, 201, 450, 629, 884, 1159
DpnI GATC 1 cut(s) 556
DpnII GATC 1 cut(s) 554
Eam1104I CTCTTC 4 cut(s) 62, 644, 1121, 1240
EarI CTCTTC 4 cut(s) 62, 644, 1121, 1240
Eco130I CCWWGG 1 cut(s) 969
Eco147I AGGCCT 1 cut(s) 667
Eco24I GRGCYC 1 cut(s) 1070
Eco31I GGTCTC 1 cut(s) 89
Eco47III AGCGCT 1 cut(s) 994
Eco81I CCTNAGG 1 cut(s) 629
EcoO109I RGGNCCY 1 cut(s) 83
EcoRII CCWGG 2 cut(s) 877, 1237
EcoT14I CCWWGG 1 cut(s) 969
EcoT22I ATGCAT 1 cut(s) 810
EcoT38I GRGCYC 1 cut(s) 1070
ErhI CCWWGG 1 cut(s) 969
FaeI CATG 9 cut(s) 133, 459, 529, 707, 812, 973, 1066, 1122, 1254
FalI AAGNNNNNCTT 2 cut(s) 1142, 1174
FaqI GGGAC 1 cut(s) 685
FatI CATG 9 cut(s) 129, 455, 525, 703, 808, 969, 1062, 1118, 1250
FauI CCCGC 1 cut(s) 2
FauNDI CATATG 1 cut(s) 598
FblI GTMKAC 2 cut(s) 737, 1192
Fnu4HI GCNGC 4 cut(s) 534, 624, 813, 942
FokI GGATG 3 cut(s) 179, 653, 1196
FriOI GRGCYC 1 cut(s) 1070
Fsp4HI GCNGC 4 cut(s) 534, 624, 813, 942
FspBI CTAG 2 cut(s) 996, 1218
GlaI GCGC 2 cut(s) 994, 1058
GluI GCNGC 4 cut(s) 534, 624, 813, 942
HaeII RGCGCY 1 cut(s) 996
HaeIII GGCC 4 cut(s) 85, 258, 667, 968
HgaI GACGC 1 cut(s) 349
HhaI GCGC 2 cut(s) 995, 1059
Hin1II CATG 9 cut(s) 133, 459, 529, 707, 812, 973, 1066, 1122, 1254
Hin6I GCGC 2 cut(s) 993, 1057
HinP1I GCGC 2 cut(s) 993, 1057
HincII GTYRAC 1 cut(s) 1193
HindII GTYRAC 1 cut(s) 1193
HindIII AAGCTT 2 cut(s) 538, 926
Hpy166II GTNNAC 6 cut(s) 494, 658, 684, 738, 934, 1193
Hpy188I TCNGA 2 cut(s) 453, 1103
Hpy188III TCNNGA 2 cut(s) 425, 819
Hpy8I GTNNAC 6 cut(s) 494, 658, 684, 738, 934, 1193
HpyAV CCTTC 4 cut(s) 148, 751, 789, 958
HpyCH4III ACNGT 2 cut(s) 581, 1107
HpyCH4IV ACGT 1 cut(s) 936
HpyCH4V TGCA 8 cut(s) 313, 529, 536, 707, 731, 808, 866, 1185
HpyF10VI GCNNNNNNNGC 2 cut(s) 1054, 1065
HpyF3I CTNAG 6 cut(s) 48, 201, 450, 629, 884, 1159
HpySE526I ACGT 1 cut(s) 936
Hsp92II CATG 9 cut(s) 133, 459, 529, 707, 812, 973, 1066, 1122, 1254
HspAI GCGC 2 cut(s) 993, 1057
Kzo9I GATC 1 cut(s) 554
LguI GCTCTTC 1 cut(s) 1240
Lsp1109I GCAGC 4 cut(s) 520, 610, 799, 928
LweI GCATC 3 cut(s) 610, 897, 1224
MabI ACCWGGT 1 cut(s) 877
MaeI CTAG 2 cut(s) 996, 1218
MaeII ACGT 1 cut(s) 936
MaeIII GTNAC 1 cut(s) 227
MalI GATC 1 cut(s) 556
MboI GATC 1 cut(s) 554
MboII GAAGA 5 cut(s) 79, 430, 661, 1138, 1254
MhlI GDGCHC 1 cut(s) 1070
MluCI AATT 6 cut(s) 89, 101, 116, 460, 614, 1090
MlyI GAGTC 5 cut(s) 127, 602, 707, 1124, 1151
Mph1103I ATGCAT 1 cut(s) 810
MseI TTAA 3 cut(s) 162, 414, 872
MslI CAYNNNNRTG 1 cut(s) 790
MspR9I CCNGG 2 cut(s) 879, 1239
Mva1269I GAATGC 1 cut(s) 37
MvaI CCWGG 2 cut(s) 879, 1239
MvnI CGCG 1 cut(s) 360
MwoI GCNNNNNNNGC 2 cut(s) 1054, 1065
NcoI CCATGG 1 cut(s) 969
NdeI CATATG 1 cut(s) 598
NdeII GATC 1 cut(s) 554
NlaIII CATG 9 cut(s) 133, 459, 529, 707, 812, 973, 1066, 1122, 1254
NlaIV GGNNCC 1 cut(s) 84
NmeAIII GCCGAG 1 cut(s) 249
NmuCI GTSAC 1 cut(s) 227
NsiI ATGCAT 1 cut(s) 810
NspI RCATGY 3 cut(s) 459, 529, 812
PaeI GCATGC 2 cut(s) 529, 812
PceI AGGCCT 1 cut(s) 667
PciI ACATGT 1 cut(s) 455
PciSI GCTCTTC 1 cut(s) 1240
PctI GAATGC 1 cut(s) 37
PfeI GAWTC 5 cut(s) 285, 317, 421, 428, 830
PflFI GACNNNGTC 1 cut(s) 879
PkrI GCNGC 4 cut(s) 535, 625, 814, 943
PleI GAGTC 5 cut(s) 127, 601, 706, 1123, 1150
PpsI GAGTC 5 cut(s) 127, 601, 706, 1123, 1150
PscI ACATGT 1 cut(s) 455
PsiI TTATAA 2 cut(s) 501, 1097
Psp6I CCWGG 2 cut(s) 877, 1237
PspGI CCWGG 2 cut(s) 877, 1237
PspN4I GGNNCC 1 cut(s) 84
PspPI GGNCC 1 cut(s) 83
PsyI GACNNNGTC 1 cut(s) 879
RsaI GTAC 2 cut(s) 683, 889
RsaNI GTAC 2 cut(s) 682, 888
RseI CAYNNNNRTG 1 cut(s) 790
SalI GTCGAC 1 cut(s) 1191
SapI GCTCTTC 1 cut(s) 1240
SaqAI TTAA 3 cut(s) 162, 414, 872
SatI GCNGC 4 cut(s) 534, 624, 813, 942
Sau3AI GATC 1 cut(s) 554
Sau96I GGNCC 1 cut(s) 83
ScaI AGTACT 1 cut(s) 889
SchI GAGTC 5 cut(s) 127, 602, 707, 1124, 1151
ScrFI CCNGG 2 cut(s) 879, 1239
SduI GDGCHC 1 cut(s) 1070
SexAI ACCWGGT 1 cut(s) 877
SfaNI GCATC 3 cut(s) 610, 897, 1224
SmiMI CAYNNNNRTG 1 cut(s) 790
SmlI CTYRAG 2 cut(s) 144, 956
SmoI CTYRAG 2 cut(s) 144, 956
SphI GCATGC 2 cut(s) 529, 812
Sse9I AATT 6 cut(s) 89, 101, 116, 460, 614, 1090
SseBI AGGCCT 1 cut(s) 667
SsiI CCGC 2 cut(s) 9, 1048
SspMI CTAG 2 cut(s) 996, 1218
StuI AGGCCT 1 cut(s) 667
StyD4I CCNGG 2 cut(s) 877, 1237
StyI CCWWGG 1 cut(s) 969
TaaI ACNGT 2 cut(s) 581, 1107
TaiI ACGT 1 cut(s) 939
TaqI TCGA 3 cut(s) 193, 1113, 1192
TasI AATT 6 cut(s) 89, 101, 116, 460, 614, 1090
TatI WGTACW 2 cut(s) 681, 887
TfiI GAWTC 5 cut(s) 285, 317, 421, 428, 830
Tru1I TTAA 3 cut(s) 162, 414, 872
Tru9I TTAA 3 cut(s) 162, 414, 872
TseFI GTSAC 1 cut(s) 227
TseI GCWGC 4 cut(s) 533, 623, 812, 941
Tsp45I GTSAC 1 cut(s) 227
TspDTI ATGAA 7 cut(s) 318, 534, 662, 854, 977, 1051, 1139
Tth111I GACNNNGTC 1 cut(s) 879
XceI RCATGY 3 cut(s) 459, 529, 812
XmiI GTMKAC 2 cut(s) 737, 1192
XspI CTAG 2 cut(s) 996, 1218
ZrmI AGTACT 1 cut(s) 889
Zsp2I ATGCAT 1 cut(s) 810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.