MD10G1313000.v1.1

Salt stress response/antifungal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0024230
Physical Location & Seq
Reverse (-)
39776066 .. 39779806
3741 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1313000.v1.1.491

Sequence Viewer

Length: 1554 bp
ATGGCAATTATGATGCTTTTGTTTATCTTCATCTGCATGCTTAGCTTGCTCGGCTGCCCGGGCACTACTACTATTACCGCAAAAGCTTTTGATCAGATCACCCGTGGTACGTGCGTAAGCCAAGTCGGGGGCAGCAATGGGGACGCGGAGTTCAGATTCAATCTCAACAACCTGCTCTCCACTACCCTTTCCTCCAATGCCGACCATGGCTATCACAATGCCACCTACGTAGGCACAAACTCAGCTGAAAAAGTCTACGTCTCCTTCCTCTGTCGCGGTGATGTCACCTCAGACATTTGTAAAGAATGTGTGGACACCGCAACCACCTTCGCCTTTCAAAATTGCCCCCATGCAACAGATGCTGTACTATGGTTCGACAAATGCTTGTATCGAATCTCCAATGACTATGCATGGATACATCCCATGGCGAACAACTCACGTAGAGACTACGTCAAAGTTTTGACGGGGAAGAATGTGATAACCAAACCCATCAGCCGCTATAATCGGTTGCCCGCAGCTACTATGAATAAATTAGTCACTGAGGCTGCCAAAGCTGACAGATTGTTCACTACCAAAGAAGCCCAAGTTAAAGGGTCAAATATGACGCTGTACAGCCTTGCGCAGTGCACGCCCGCAGCTGATTGCAATCGGTGTCTTCGAGCGGCCGTAGCAGATATAGTTTCAAGGGAAGACCAGTTAGAGGGATTTTTTCTATATCAGAATTGTAATATTAGGTACCAACTTTCCCCGTTCTTCAAAAAATTAAGGACTGTAGCAGCTGGATCAAGGAGCAAACTGATCTTGTACATTGTTGCCGTTGGAACATTAGGTAACGGGACAGAAATAGCGGTAAAAAAACTGTCAAAAAGCTCCAAACAAGGTGTAAAAGAATTTAAGAATGAGGTAGCACTAGTTGCCAAGCTTCAACACAGAAATCTGGTGCGGCAGCTAGGCTTTTGCTTGGAAGGAGAAGAAGCCATAGTTGTGTATGAATACGTGCCTAAAAAAAGTCTTGATCATTTTCTATTGGAACACAAACGACGTGAAAAACTGGATTGGTCAAGACGTCGCGTGATAATTGATGGAATTGCTCAGGGAGTTCTATATCTCCATGAAGATTCAAGGCTTAGAGTTATACATCGTGATTTGAAAGCTAGCAATATTCTTTTAGATGATGATATGAATCTAATAATATCAGATTTTGGAATGGCAAAAGTGTTTGGAGTTGATGATCAAACTCACAGAAACACAAGCAGAATTTATACTACGAAGGGATTGTATTCCACACAATCCGATATCTTTCGCTTTGGGGTACTCTTGCTTGAGATCCTAACAGGGAGAAGGAACTTTTTGGGCTTTCATGTCACAAATTGTGCACCAACTCTTGTAGGATATGCTTGGGAATTATGGAACGAAGGGAAAGGGTTGGAGTTGATGGATCCATTATTGAAAGACTCATGTAATACCAATGAGTTCTTGAGATGCATCCATGTCAGATTATTGTGTGTTCAAGAGGATGCAAACAAAAGACAACCATGTCGTCGGTTGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase_Tyr Stress-antifung

Protein Analysis

518

Amino Acids

58.37

Weight (kDa)

8.98

Isoelectric Point (pI)

34.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 38 - 134 5.8e-18 Salt stress response/antifungal
Stress-antifung PF01657 162 - 247 1.7e-07 Salt stress response/antifungal
PK_Tyr_Ser-Thr PF07714 257 - 453 3.1e-34 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 277 - 451 7.5e-30 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024230)

Species Orthologous Gene IDs
malus_domestica MD00G1103000.v1.1 MD10G1313000.v1.1
rosa_rugosa Rorug06G0063900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1536
AatII GACGTC 1 cut(s) 1069
Acc16I TGCGCA 1 cut(s) 621
Acc36I ACCTGC 1 cut(s) 180
Acc65I GGTACC 1 cut(s) 735
AccB1I GGYRCC 1 cut(s) 735
AccBSI CCGCTC 1 cut(s) 662
AccI GTMKAC 1 cut(s) 255
AccII CGCG 3 cut(s) 146, 276, 1071
AclWI GGATC 4 cut(s) 790, 1321, 1433, 1446
AcoI YGGCCR 1 cut(s) 663
AcsI RAATTY 2 cut(s) 890, 1257
AcyI GRCGYC 1 cut(s) 1066
AfaI GTAC 6 cut(s) 109, 366, 611, 737, 806, 1314
AfiI CCNNNNNNNGG 2 cut(s) 127, 700
AgsI TTSAA 9 cut(s) 160, 338, 684, 757, 926, 1122, 1150, 1450, 1511
AhlI ACTAGT 1 cut(s) 910
AjiI CACGTC 1 cut(s) 1043
AloI GAACNNNNNNTCC 2 cut(s) 134, 166
Alw21I GWGCWC 2 cut(s) 629, 1378
Alw26I GTCTC 2 cut(s) 265, 438
Alw44I GTGCAC 2 cut(s) 625, 1374
AlwI GGATC 4 cut(s) 790, 1321, 1433, 1446
AlwNI CAGNNNCTG 1 cut(s) 362
Ama87I CYCGRG 1 cut(s) 58
AoxI GGCC 1 cut(s) 663
ApaLI GTGCAC 2 cut(s) 625, 1374
ApeKI GCWGC 7 cut(s) 54, 132, 515, 545, 635, 776, 946
ApoI RAATTY 2 cut(s) 890, 1257
Asp718I GGTACC 1 cut(s) 735
AspLEI GCGC 1 cut(s) 622
AsuC2I CCSGG 2 cut(s) 59, 60
AsuHPI GGTGA 3 cut(s) 91, 277, 290
AsuNHI GCTAGC 1 cut(s) 1154
AvaI CYCGRG 1 cut(s) 58
BaeGI GKGCMC 3 cut(s) 65, 629, 1378
BaeI ACNNNNGTAYC 2 cut(s) 99, 132
BamHI GGATCC 1 cut(s) 1438
BanI GGYRCC 1 cut(s) 735
BbsI GAAGAC 2 cut(s) 647, 696
Bbv12I GWGCWC 2 cut(s) 629, 1378
BbvI GCAGC 7 cut(s) 41, 144, 527, 532, 647, 788, 958
BccI CCATC 3 cut(s) 497, 1076, 1429
BceAI ACGGC 2 cut(s) 650, 800
BciVI GTATCC 1 cut(s) 408
BclI TGATCA 3 cut(s) 91, 1015, 1231
BcnI CCSGG 2 cut(s) 59, 60
BcoDI GTCTC 2 cut(s) 265, 438
BcuI ACTAGT 1 cut(s) 910
BfaI CTAG 3 cut(s) 911, 950, 1155
BfmI CTRYAG 1 cut(s) 771
BfuAI ACCTGC 1 cut(s) 180
BfuI GTATCC 1 cut(s) 408
BlpI GCTNAGC 1 cut(s) 41
Bme1390I CCNGG 2 cut(s) 59, 60
BmeT110I CYCGRG 1 cut(s) 58
BmgBI CACGTC 1 cut(s) 1043
BmiI GGNNCC 2 cut(s) 737, 1440
BmrFI CCNGG 2 cut(s) 59, 60
BmsI GCATC 5 cut(s) 3, 349, 1472, 1494, 1507
BmtI GCTAGC 1 cut(s) 1158
BpiI GAAGAC 2 cut(s) 647, 696
Bpu10I CCTNAGC 1 cut(s) 1092
Bpu1102I GCTNAGC 1 cut(s) 41
BpuEI CTTGAG 2 cut(s) 1343, 1498
BpuMI CCSGG 2 cut(s) 59, 60
BsaAI YACGTR 4 cut(s) 111, 229, 440, 997
BsaBI GATNNNNATC 2 cut(s) 645, 1182
BsaHI GRCGYC 1 cut(s) 1066
BsaJI CCNNGG 4 cut(s) 58, 103, 205, 423
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 2 cut(s) 127, 700
Bse1I ACTGG 2 cut(s) 694, 1056
Bse3DI GCAATG 1 cut(s) 142
Bse8I GATNNNNATC 2 cut(s) 645, 1182
BseDI CCNNGG 4 cut(s) 58, 103, 205, 423
BseGI GGATG 3 cut(s) 418, 1485, 1522
BseJI GATNNNNATC 2 cut(s) 645, 1182
BseLI CCNNNNNNNGG 2 cut(s) 127, 700
BseMI GCAATG 1 cut(s) 142
BseMII CTCAG 4 cut(s) 255, 303, 531, 1106
BseNI ACTGG 2 cut(s) 694, 1056
BseSI GKGCMC 3 cut(s) 65, 629, 1378
BseX3I CGGCCG 1 cut(s) 663
BseXI GCAGC 7 cut(s) 41, 144, 527, 532, 647, 788, 958
Bsh1236I CGCG 3 cut(s) 146, 276, 1071
Bsh1285I CGRYCG 1 cut(s) 666
BshFI GGCC 1 cut(s) 665
BshNI GGYRCC 1 cut(s) 735
BsiEI CGRYCG 1 cut(s) 666
BsiHKAI GWGCWC 2 cut(s) 629, 1378
BsiHKCI CYCGRG 1 cut(s) 58
BsiSI CCGG 1 cut(s) 59
BslFI GGGAC 2 cut(s) 155, 850
BslI CCNNNNNNNGG 2 cut(s) 127, 700
BsmAI GTCTC 2 cut(s) 265, 438
BsmBI CGTCTC 1 cut(s) 265
BsmFI GGGAC 2 cut(s) 155, 850
BsnI GGCC 1 cut(s) 665
BsoBI CYCGRG 1 cut(s) 58
Bsp1286I GDGCHC 3 cut(s) 65, 629, 1378
Bsp1407I TGTACA 2 cut(s) 609, 804
Bsp143I GATC 8 cut(s) 91, 96, 782, 798, 1015, 1231, 1326, 1438
Bsp1720I GCTNAGC 1 cut(s) 41
Bsp19I CCATGG 2 cut(s) 205, 423
BspANI GGCC 1 cut(s) 665
BspCNI CTCAG 4 cut(s) 254, 302, 532, 1105
BspFNI CGCG 3 cut(s) 146, 276, 1071
BspLI GGNNCC 2 cut(s) 737, 1440
BspMI ACCTGC 1 cut(s) 180
BspOI GCTAGC 1 cut(s) 1158
BspPI GGATC 4 cut(s) 790, 1321, 1433, 1446
BspT107I GGYRCC 1 cut(s) 735
BsrBI CCGCTC 1 cut(s) 662
BsrDI GCAATG 1 cut(s) 142
BsrGI TGTACA 2 cut(s) 609, 804
BsrI ACTGG 2 cut(s) 694, 1056
BssECI CCNNGG 4 cut(s) 58, 103, 205, 423
BssMI GATC 8 cut(s) 91, 96, 782, 798, 1015, 1231, 1326, 1438
BssNI GRCGYC 1 cut(s) 1066
BssT1I CCWWGG 2 cut(s) 205, 423
Bst4CI ACNGT 2 cut(s) 772, 861
BstACI GRCGYC 1 cut(s) 1066
BstAPI GCANNNNNTGC 2 cut(s) 359, 914
BstAUI TGTACA 2 cut(s) 609, 804
BstBAI YACGTR 4 cut(s) 111, 229, 440, 997
BstC8I GCNNGC 6 cut(s) 38, 47, 513, 629, 633, 1156
BstDEI CTNAG 6 cut(s) 41, 241, 289, 540, 1092, 1127
BstDSI CCRYGG 3 cut(s) 103, 205, 423
BstF5I GGATG 3 cut(s) 418, 1485, 1522
BstFNI CGCG 3 cut(s) 146, 276, 1071
BstHHI GCGC 1 cut(s) 622
BstKTI GATC 8 cut(s) 94, 99, 785, 801, 1018, 1234, 1329, 1441
BstMAI GTCTC 2 cut(s) 265, 438
BstMBI GATC 8 cut(s) 91, 96, 782, 798, 1015, 1231, 1326, 1438
BstMCI CGRYCG 1 cut(s) 666
BstMWI GCNNNNNNNGC 9 cut(s) 42, 46, 51, 60, 359, 551, 628, 668, 914
BstNSI RCATGY 1 cut(s) 40
BstSCI CCNGG 2 cut(s) 57, 58
BstSFI CTRYAG 1 cut(s) 771
BstSLI GKGCMC 3 cut(s) 65, 629, 1378
BstSNI TACGTA 1 cut(s) 229
BstUI CGCG 3 cut(s) 146, 276, 1071
BstV1I GCAGC 7 cut(s) 41, 144, 527, 532, 647, 788, 958
BstV2I GAAGAC 2 cut(s) 647, 696
BstX2I RGATCY 2 cut(s) 1326, 1438
BstYI RGATCY 2 cut(s) 1326, 1438
BstZI CGGCCG 1 cut(s) 663
BsuI GTATCC 1 cut(s) 408
BsuRI GGCC 1 cut(s) 665
BtgI CCRYGG 3 cut(s) 103, 205, 423
BtrI CACGTC 1 cut(s) 1043
BtsCI GGATG 3 cut(s) 418, 1485, 1522
BtsI GCAGTG 1 cut(s) 629
BtsIMutI CAGTG 2 cut(s) 537, 629
BveI ACCTGC 1 cut(s) 180
Cac8I GCNNGC 6 cut(s) 38, 47, 513, 629, 633, 1156
CaiI CAGNNNCTG 1 cut(s) 362
CfoI GCGC 1 cut(s) 622
Cfr9I CCCGGG 1 cut(s) 58
CseI GACGC 2 cut(s) 152, 613
Csp6I GTAC 6 cut(s) 108, 365, 610, 736, 805, 1313
CviQI GTAC 6 cut(s) 108, 365, 610, 736, 805, 1313
DdeI CTNAG 6 cut(s) 41, 241, 289, 540, 1092, 1127
DpnI GATC 8 cut(s) 93, 98, 784, 800, 1017, 1233, 1328, 1440
DpnII GATC 8 cut(s) 91, 96, 782, 798, 1015, 1231, 1326, 1438
DrdI GACNNNNNNGTC 1 cut(s) 1536
DseDI GACNNNNNNGTC 1 cut(s) 1536
EaeI YGGCCR 1 cut(s) 663
EagI CGGCCG 1 cut(s) 663
EclXI CGGCCG 1 cut(s) 663
Eco105I TACGTA 1 cut(s) 229
Eco130I CCWWGG 2 cut(s) 205, 423
Eco32I GATATC 1 cut(s) 1297
Eco52I CGGCCG 1 cut(s) 663
Eco88I CYCGRG 1 cut(s) 58
EcoRV GATATC 1 cut(s) 1297
EcoT14I CCWWGG 2 cut(s) 205, 423
EcoT22I ATGCAT 2 cut(s) 412, 1487
ErhI CCWWGG 2 cut(s) 205, 423
Esp3I CGTCTC 1 cut(s) 265
FaqI GGGAC 2 cut(s) 155, 850
FauI CCCGC 2 cut(s) 520, 640
FbaI TGATCA 3 cut(s) 91, 1015, 1231
FblI GTMKAC 1 cut(s) 255
FokI GGATG 3 cut(s) 405, 1472, 1529
FspBI CTAG 3 cut(s) 911, 950, 1155
FspI TGCGCA 1 cut(s) 621
GlaI GCGC 1 cut(s) 621
HaeIII GGCC 1 cut(s) 665
HapII CCGG 1 cut(s) 59
HgaI GACGC 2 cut(s) 152, 613
HhaI GCGC 1 cut(s) 622
Hin1I GRCGYC 1 cut(s) 1066
Hin6I GCGC 1 cut(s) 620
HinP1I GCGC 1 cut(s) 620
HindIII AAGCTT 2 cut(s) 84, 920
HinfI GANTC 5 cut(s) 156, 393, 1118, 1183, 1454
HpaII CCGG 1 cut(s) 59
HphI GGTGA 3 cut(s) 91, 277, 290
Hpy166II GTNNAC 5 cut(s) 256, 313, 567, 627, 1376
Hpy188I TCNGA 7 cut(s) 96, 155, 292, 720, 1198, 1294, 1496
Hpy188III TCNNGA 5 cut(s) 1013, 1062, 1142, 1477, 1511
Hpy8I GTNNAC 5 cut(s) 256, 313, 567, 627, 1376
Hpy99I CGWCG 3 cut(s) 1044, 1071, 1545
HpyAV CCTTC 6 cut(s) 274, 337, 959, 1264, 1335, 1409
HpyCH4III ACNGT 2 cut(s) 772, 861
HpyCH4IV ACGT 8 cut(s) 110, 228, 258, 439, 450, 996, 1042, 1066
HpyCH4V TGCA 8 cut(s) 36, 353, 410, 627, 645, 1376, 1485, 1520
HpyF10VI GCNNNNNNNGC 9 cut(s) 42, 46, 51, 60, 359, 551, 628, 668, 914
HpyF3I CTNAG 6 cut(s) 41, 241, 289, 540, 1092, 1127
HpySE526I ACGT 8 cut(s) 110, 228, 258, 439, 450, 996, 1042, 1066
Hsp92I GRCGYC 1 cut(s) 1066
HspAI GCGC 1 cut(s) 620
KpnI GGTACC 1 cut(s) 739
Ksp22I TGATCA 3 cut(s) 91, 1015, 1231
Kzo9I GATC 8 cut(s) 91, 96, 782, 798, 1015, 1231, 1326, 1438
LmnI GCTCC 2 cut(s) 789, 875
LpnPI CCDG 8 cut(s) 72, 185, 707, 765, 923, 1037, 1079, 1320
Lsp1109I GCAGC 7 cut(s) 41, 144, 527, 532, 647, 788, 958
LweI GCATC 5 cut(s) 3, 349, 1472, 1494, 1507
MaeI CTAG 3 cut(s) 911, 950, 1155
MaeII ACGT 8 cut(s) 110, 228, 258, 439, 450, 996, 1042, 1066
MaeIII GTNAC 4 cut(s) 283, 535, 830, 1363
MalI GATC 8 cut(s) 93, 98, 784, 800, 1017, 1233, 1328, 1440
MbiI CCGCTC 1 cut(s) 662
MboI GATC 8 cut(s) 91, 96, 782, 798, 1015, 1231, 1326, 1438
MboII GAAGA 7 cut(s) 19, 481, 647, 701, 745, 983, 1127
MflI RGATCY 2 cut(s) 1326, 1438
MhlI GDGCHC 3 cut(s) 65, 629, 1378
MlyI GAGTC 1 cut(s) 1448
MmeI TCCRAC 2 cut(s) 799, 1407
MnlI CCTC 7 cut(s) 202, 278, 298, 535, 694, 895, 1507
Mph1103I ATGCAT 2 cut(s) 412, 1487
MseI TTAA 3 cut(s) 588, 764, 894
MslI CAYNNNNRTG 2 cut(s) 35, 983
MspA1I CMGCKG 3 cut(s) 245, 638, 779
MspI CCGG 1 cut(s) 59
MspR9I CCNGG 2 cut(s) 59, 60
MvnI CGCG 3 cut(s) 146, 276, 1071
MwoI GCNNNNNNNGC 9 cut(s) 42, 46, 51, 60, 359, 551, 628, 668, 914
NciI CCSGG 2 cut(s) 59, 60
NcoI CCATGG 2 cut(s) 205, 423
NdeII GATC 8 cut(s) 91, 96, 782, 798, 1015, 1231, 1326, 1438
NheI GCTAGC 1 cut(s) 1154
NlaIV GGNNCC 2 cut(s) 737, 1440
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 3 cut(s) 283, 535, 1363
NsbI TGCGCA 1 cut(s) 621
NsiI ATGCAT 2 cut(s) 412, 1487
NspI RCATGY 1 cut(s) 40
PaeI GCATGC 1 cut(s) 40
PcsI WCGNNNNNNNCGW 1 cut(s) 655
PfeI GAWTC 4 cut(s) 156, 393, 1118, 1183
PflFI GACNNNGTC 1 cut(s) 449
PleI GAGTC 1 cut(s) 1448
PpsI GAGTC 1 cut(s) 1448
Ppu21I YACGTR 4 cut(s) 111, 229, 440, 997
PspN4I GGNNCC 2 cut(s) 737, 1440
PstNI CAGNNNCTG 1 cut(s) 362
PsuI RGATCY 2 cut(s) 1326, 1438
PsyI GACNNNGTC 1 cut(s) 449
PvuII CAGCTG 3 cut(s) 245, 638, 779
RsaI GTAC 6 cut(s) 109, 366, 611, 737, 806, 1314
RsaNI GTAC 6 cut(s) 108, 365, 610, 736, 805, 1313
RseI CAYNNNNRTG 2 cut(s) 35, 983
SaqAI TTAA 3 cut(s) 588, 764, 894
Sau3AI GATC 8 cut(s) 91, 96, 782, 798, 1015, 1231, 1326, 1438
SchI GAGTC 1 cut(s) 1448
ScrFI CCNGG 2 cut(s) 59, 60
SduI GDGCHC 3 cut(s) 65, 629, 1378
SfaNI GCATC 5 cut(s) 3, 349, 1472, 1494, 1507
SfcI CTRYAG 1 cut(s) 771
SmaI CCCGGG 1 cut(s) 60
SmiMI CAYNNNNRTG 2 cut(s) 35, 983
SmlI CTYRAG 2 cut(s) 1322, 1477
SmoI CTYRAG 2 cut(s) 1322, 1477
SnaBI TACGTA 1 cut(s) 229
SpeI ACTAGT 1 cut(s) 910
SphI GCATGC 1 cut(s) 40
SrfI GCCCGGGC 1 cut(s) 60
SspI AATATT 2 cut(s) 730, 1162
SspMI CTAG 3 cut(s) 911, 950, 1155
StyD4I CCNGG 2 cut(s) 57, 58
StyI CCWWGG 2 cut(s) 205, 423
TaaI ACNGT 2 cut(s) 772, 861
TaiI ACGT 8 cut(s) 113, 231, 261, 442, 453, 999, 1045, 1069
TaqI TCGA 3 cut(s) 375, 391, 658
TatI WGTACW 3 cut(s) 364, 609, 804
TauI GCSGC 3 cut(s) 498, 665, 946
TfiI GAWTC 4 cut(s) 156, 393, 1118, 1183
Tru1I TTAA 3 cut(s) 588, 764, 894
Tru9I TTAA 3 cut(s) 588, 764, 894
TscAI CASTG 2 cut(s) 544, 629
TseFI GTSAC 3 cut(s) 283, 535, 1363
TseI GCWGC 7 cut(s) 54, 132, 515, 545, 635, 776, 946
Tsp45I GTSAC 3 cut(s) 283, 535, 1363
TspDTI ATGAA 6 cut(s) 19, 539, 1005, 1128, 1196, 1349
TspMI CCCGGG 1 cut(s) 58
TspRI CASTG 2 cut(s) 544, 629
Tth111I GACNNNGTC 1 cut(s) 449
VneI GTGCAC 2 cut(s) 625, 1374
XapI RAATTY 2 cut(s) 890, 1257
XceI RCATGY 1 cut(s) 40
XmaI CCCGGG 1 cut(s) 58
XmiI GTMKAC 1 cut(s) 255
XspI CTAG 3 cut(s) 911, 950, 1155
ZraI GACGTC 1 cut(s) 1067
Zsp2I ATGCAT 2 cut(s) 412, 1487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.