MD10G1324900.v1.1

stigma-specific Stig1 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0020348
Physical Location & Seq
Reverse (-)
40569280 .. 40569693
414 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1324900.v1.1.491

Sequence Viewer

Length: 414 bp
ATGAAACATTCTCTCAATGTTTTGTTTATGCTAGCCATGCTGATTATGGCTTTAGCCATTTCTCTCTCTGCAACACCAAATAACCACGAAGAAGCAGCATTCATCAGGAGATCATCAAGAAGCCGGTTCCTTGCCTCGAATACTCCAGCGGGTCGGGGAGGATGTGGCCAAAACCCTCTTGCTTGCAGTGCCACTAAAGGTAGCCGAGGGCCAGATTGTTGCAACAATGTTTGTGCCGATTTGAGGACAGACGCATTCAACTGCGGGGTTTGTGGGAAGAGATGCACCAGTTCAGAGATGTGCTGCAACTCTAATTGTGTCAAACCAATGTCCGACCATCAAAACTGTGGCAAGTGCTCCAACCGTTGCAAGATAGGCACTTCTTGCCACAAAGGGTTTTGCGACTATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

138

Amino Acids

14.61

Weight (kDa)

8.84

Isoelectric Point (pI)

47.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 54 - 137 9.9e-21 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020348)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g17590
malus_domestica MD10G1324900.v1.1
prunus_persica Prupe.4G017300_v2.0.a1 Prupe.4G020700_v2.0.a1 Prupe.4G199900_v2.0.a1 Prupe.4G239000_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 149, 264
AcoI YGGCCR 1 cut(s) 166
AfiI CCNNNNNNNGG 1 cut(s) 243
AgsI TTSAA 1 cut(s) 259
Alw21I GWGCWC 1 cut(s) 359
AoxI GGCC 2 cut(s) 166, 209
ApeKI GCWGC 2 cut(s) 95, 303
AspS9I GGNCC 1 cut(s) 209
AsuNHI GCTAGC 1 cut(s) 31
BalI TGGCCA 1 cut(s) 168
Bbv12I GWGCWC 1 cut(s) 359
BbvI GCAGC 2 cut(s) 107, 290
BccI CCATC 1 cut(s) 345
BfaI CTAG 1 cut(s) 32
BisI GCNGC 2 cut(s) 96, 304
BlsI GCNGC 2 cut(s) 97, 305
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 1 cut(s) 128
BmsI GCATC 1 cut(s) 272
BmtI GCTAGC 1 cut(s) 35
BpmI CTGGAG 1 cut(s) 129
BsaJI CCNNGG 1 cut(s) 205
Bsc4I CCNNNNNNNGG 1 cut(s) 243
Bse118I RCCGGY 1 cut(s) 123
Bse1I ACTGG 1 cut(s) 288
BseDI CCNNGG 1 cut(s) 205
BseGI GGATG 1 cut(s) 167
BseLI CCNNNNNNNGG 1 cut(s) 243
BseNI ACTGG 1 cut(s) 288
BseXI GCAGC 2 cut(s) 107, 290
BshFI GGCC 2 cut(s) 168, 211
BsiHKAI GWGCWC 1 cut(s) 359
BsiSI CCGG 1 cut(s) 124
BslI CCNNNNNNNGG 1 cut(s) 243
BsmI GAATGC 2 cut(s) 98, 254
BsnI GGCC 2 cut(s) 168, 211
Bsp1286I GDGCHC 1 cut(s) 359
Bsp143I GATC 1 cut(s) 110
BspACI CCGC 2 cut(s) 149, 264
BspANI GGCC 2 cut(s) 168, 211
BspLI GGNNCC 1 cut(s) 128
BspOI GCTAGC 1 cut(s) 35
BsrFI RCCGGY 1 cut(s) 123
BsrI ACTGG 1 cut(s) 288
BssAI RCCGGY 1 cut(s) 123
BssECI CCNNGG 1 cut(s) 205
BssMI GATC 1 cut(s) 110
Bst4CI ACNGT 2 cut(s) 347, 365
Bst6I CTCTTC 1 cut(s) 272
BstAPI GCANNNNNTGC 1 cut(s) 384
BstC8I GCNNGC 2 cut(s) 33, 184
BstDEI CTNAG 1 cut(s) 411
BstF5I GGATG 1 cut(s) 167
BstKTI GATC 1 cut(s) 113
BstMBI GATC 1 cut(s) 110
BstMWI GCNNNNNNNGC 4 cut(s) 37, 188, 375, 384
BstV1I GCAGC 2 cut(s) 107, 290
BsuRI GGCC 2 cut(s) 168, 211
BtsCI GGATG 1 cut(s) 167
BtsI GCAGTG 1 cut(s) 193
BtsIMutI CAGTG 1 cut(s) 193
Cac8I GCNNGC 2 cut(s) 33, 184
Cfr10I RCCGGY 1 cut(s) 123
Cfr13I GGNCC 1 cut(s) 209
CseI GACGC 1 cut(s) 260
CviAII CATG 1 cut(s) 37
CviJI RGCY 7 cut(s) 35, 50, 56, 123, 168, 204, 211
CviKI_1 RGCY 7 cut(s) 35, 50, 56, 123, 168, 204, 211
DdeI CTNAG 1 cut(s) 411
DpnI GATC 1 cut(s) 112
DpnII GATC 1 cut(s) 110
EaeI YGGCCR 1 cut(s) 166
Eam1104I CTCTTC 1 cut(s) 272
EarI CTCTTC 1 cut(s) 272
FaeI CATG 1 cut(s) 40
FaiI YATR 4 cut(s) 29, 38, 47, 408
FatI CATG 1 cut(s) 36
FauI CCCGC 2 cut(s) 142, 257
Fnu4HI GCNGC 2 cut(s) 96, 304
FokI GGATG 1 cut(s) 174
Fsp4HI GCNGC 2 cut(s) 96, 304
FspBI CTAG 1 cut(s) 32
GluI GCNGC 2 cut(s) 96, 304
GsuI CTGGAG 1 cut(s) 129
HaeIII GGCC 2 cut(s) 168, 211
HapII CCGG 1 cut(s) 124
HgaI GACGC 1 cut(s) 260
Hin1II CATG 1 cut(s) 40
HpaII CCGG 1 cut(s) 124
Hpy188I TCNGA 2 cut(s) 295, 334
Hpy188III TCNNGA 2 cut(s) 106, 117
HpyCH4III ACNGT 2 cut(s) 347, 365
HpyCH4V TGCA 6 cut(s) 71, 186, 222, 285, 306, 369
HpyF10VI GCNNNNNNNGC 4 cut(s) 37, 188, 375, 384
HpyF3I CTNAG 1 cut(s) 411
Hsp92II CATG 1 cut(s) 40
Kzo9I GATC 1 cut(s) 110
LmnI GCTCC 1 cut(s) 362
LpnPI CCDG 5 cut(s) 91, 137, 159, 225, 301
Lsp1109I GCAGC 2 cut(s) 107, 290
LweI GCATC 1 cut(s) 272
MaeI CTAG 1 cut(s) 32
MalI GATC 1 cut(s) 112
MboI GATC 1 cut(s) 110
MboII GAAGA 2 cut(s) 101, 289
MhlI GDGCHC 1 cut(s) 359
MlsI TGGCCA 1 cut(s) 168
MluCI AATT 1 cut(s) 313
MluNI TGGCCA 1 cut(s) 168
MmeI TCCRAC 2 cut(s) 357, 384
MnlI CCTC 5 cut(s) 145, 152, 186, 200, 237
Mox20I TGGCCA 1 cut(s) 168
MscI TGGCCA 1 cut(s) 168
Msp20I TGGCCA 1 cut(s) 168
MspA1I CMGCKG 1 cut(s) 149
MspI CCGG 1 cut(s) 124
Mva1269I GAATGC 2 cut(s) 98, 254
MwoI GCNNNNNNNGC 4 cut(s) 37, 188, 375, 384
NdeII GATC 1 cut(s) 110
NheI GCTAGC 1 cut(s) 31
NlaIII CATG 1 cut(s) 40
NlaIV GGNNCC 1 cut(s) 128
NmeAIII GCCGAG 1 cut(s) 230
PctI GAATGC 2 cut(s) 98, 254
PkrI GCNGC 2 cut(s) 97, 305
PspN4I GGNNCC 1 cut(s) 128
PspPI GGNCC 1 cut(s) 209
SatI GCNGC 2 cut(s) 96, 304
Sau3AI GATC 1 cut(s) 110
Sau96I GGNCC 1 cut(s) 209
SduI GDGCHC 1 cut(s) 359
SetI ASST 1 cut(s) 202
SfaNI GCATC 1 cut(s) 272
Sse9I AATT 1 cut(s) 313
SsiI CCGC 2 cut(s) 149, 264
SspMI CTAG 1 cut(s) 32
TaaI ACNGT 2 cut(s) 347, 365
TaqI TCGA 1 cut(s) 137
TasI AATT 1 cut(s) 313
TscAI CASTG 1 cut(s) 193
TseI GCWGC 2 cut(s) 95, 303
TspDTI ATGAA 2 cut(s) 17, 91
TspRI CASTG 1 cut(s) 193
XcmI CCANNNNNNNNNTGG 2 cut(s) 43, 344
XspI CTAG 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.