MD11G1079900.v1.1

Josephin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0015189
Physical Location & Seq
Forward (+)
6837064 .. 6838065
1002 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1079900.v1.1.491

Sequence Viewer

Length: 552 bp
ATGGCGACCGACACCAATTCCCAAATCTATCACGAGCGGCAGAAGCTACAGTTTTGCCTCTTGCACTCGCTCAACAATCTTTTCCAGAAAAACGATGCATTTACTCGATCAAGCCTGAATGAGATTGCTGAAAAACTTATGCTTGAAGAACCCAACAAGGAAAAATGGACACCCTTTTCTGTGCTCTTTAAACCCCATCATAATTCACTCACTGGAAACTATGACATAAATGTGCTAATTGCTGCTTTAGAAGACAAGGGGAAGAATGTGGTTTGGCATGATCGGCGTAATGGGGCATCTTCAATTGATCTAGATGGGCCTGAAGATGCTAATTTGATGGGTATTCTGCTTAATGTTCCTGTTAGAATGTTTGCCGGGTTGTGGAAAAGTAGACATTGGGTAACGTTGAGGAAGATTGGTGGGGTTTGGTACAATTTGGATAGCGAACTTGTTGCTCCTGAGGTCTTTGAAGATGCTGAAAAGGTTAGAGAATTCTTGGATTACATGATTGGACATGGTGGAGAAGTTTTGCTTGTTATGAATAATAAATAG

Protein Analysis

184

Amino Acids

21.02

Weight (kDa)

5.79

Isoelectric Point (pI)

43.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Josephin PF02099 14 - 179 1.3e-37 Josephin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015189)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29640
fragaria_vesca FvH4_3g38250 FvH4_3g38250
malus_domestica MD11G1079900.v1.1
prunus_persica Prupe.6G060500_v2.0.a1
pyrus_communis pycom11g06740
rosa_chinensis RchiOBHm_Chr5g0068731
rosa_laevigata RLG00000036006
rosa_multiflora Rmu_sc0000376.1_g000026
rosa_roxburghii Rroxscaffold_1G00012090
rosa_rugosa Rorug05G0392700
rosa_samantha Rh5AG450200 Rh5BG468600 Rh5CG490800 Rh5DG482100
rosa_wichuraiana Rw5G042040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 37
AccI GTMKAC 1 cut(s) 391
AciI CCGC 1 cut(s) 37
AclI AACGTT 1 cut(s) 404
AcsI RAATTY 1 cut(s) 491
AcuI CTGAAG 1 cut(s) 342
AfaI GTAC 1 cut(s) 431
AfiI CCNNNNNNNGG 1 cut(s) 381
AgsI TTSAA 3 cut(s) 146, 303, 470
AluBI AGCT 1 cut(s) 46
AluI AGCT 1 cut(s) 46
Alw21I GWGCWC 1 cut(s) 186
AoxI GGCC 1 cut(s) 317
ApeKI GCWGC 1 cut(s) 242
ApoI RAATTY 1 cut(s) 491
AspS9I GGNCC 1 cut(s) 317
AsuC2I CCSGG 1 cut(s) 376
AxyI CCTNAGG 1 cut(s) 459
BauI CACGAG 1 cut(s) 32
BbsI GAAGAC 1 cut(s) 258
Bbv12I GWGCWC 1 cut(s) 186
BbvI GCAGC 1 cut(s) 229
BccI CCATC 3 cut(s) 204, 308, 331
BcgI CGANNNNNNTGC 2 cut(s) 434, 468
BcnI CCSGG 1 cut(s) 376
BfaI CTAG 1 cut(s) 311
BfmI CTRYAG 1 cut(s) 47
BisI GCNGC 2 cut(s) 38, 243
BlsI GCNGC 2 cut(s) 39, 244
Bme1390I CCNGG 1 cut(s) 376
BmgT120I GGNCC 1 cut(s) 317
BmrFI CCNGG 1 cut(s) 376
BmsI GCATC 4 cut(s) 85, 305, 316, 463
BpiI GAAGAC 1 cut(s) 258
BpuMI CCSGG 1 cut(s) 376
Bsc4I CCNNNNNNNGG 1 cut(s) 381
Bse1I ACTGG 1 cut(s) 217
Bse21I CCTNAGG 1 cut(s) 459
BseLI CCNNNNNNNGG 1 cut(s) 381
BseMII CTCAG 1 cut(s) 450
BseNI ACTGG 1 cut(s) 217
BseXI GCAGC 1 cut(s) 229
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 1 cut(s) 319
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 1 cut(s) 186
BsiSI CCGG 1 cut(s) 375
BslI CCNNNNNNNGG 1 cut(s) 381
BsnI GGCC 1 cut(s) 319
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 3 cut(s) 107, 280, 307
BspACI CCGC 1 cut(s) 37
BspANI GGCC 1 cut(s) 319
BspCNI CTCAG 1 cut(s) 451
BsrBI CCGCTC 1 cut(s) 37
BsrI ACTGG 1 cut(s) 217
BssMI GATC 3 cut(s) 107, 280, 307
BssSI CACGAG 1 cut(s) 32
Bst2BI CACGAG 1 cut(s) 32
Bst4CI ACNGT 1 cut(s) 51
BstDEI CTNAG 1 cut(s) 459
BstKTI GATC 3 cut(s) 110, 283, 310
BstMBI GATC 3 cut(s) 107, 280, 307
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 2 cut(s) 43, 283
BstSCI CCNGG 1 cut(s) 374
BstSFI CTRYAG 1 cut(s) 47
BstV1I GCAGC 1 cut(s) 229
BstV2I GAAGAC 1 cut(s) 258
Bsu36I CCTNAGG 1 cut(s) 459
BsuRI GGCC 1 cut(s) 319
BtsIMutI CAGTG 1 cut(s) 210
Cfr13I GGNCC 1 cut(s) 317
Csp6I GTAC 1 cut(s) 430
CviAII CATG 3 cut(s) 278, 505, 515
CviJI RGCY 3 cut(s) 46, 114, 319
CviKI_1 RGCY 3 cut(s) 46, 114, 319
CviQI GTAC 1 cut(s) 430
DdeI CTNAG 1 cut(s) 459
DpnI GATC 3 cut(s) 109, 282, 309
DpnII GATC 3 cut(s) 107, 280, 307
DraI TTTAAA 1 cut(s) 190
Eco57I CTGAAG 1 cut(s) 342
Eco81I CCTNAGG 1 cut(s) 459
EcoRI GAATTC 1 cut(s) 491
EcoT22I ATGCAT 1 cut(s) 100
FaeI CATG 3 cut(s) 281, 508, 518
FaiI YATR 8 cut(s) 140, 201, 222, 227, 279, 506, 516, 539
FalI AAGNNNNNCTT 2 cut(s) 516, 548
FatI CATG 3 cut(s) 277, 504, 514
FblI GTMKAC 1 cut(s) 391
Fnu4HI GCNGC 2 cut(s) 38, 243
Fsp4HI GCNGC 2 cut(s) 38, 243
FspBI CTAG 1 cut(s) 311
GluI GCNGC 2 cut(s) 38, 243
HaeIII GGCC 1 cut(s) 319
HapII CCGG 1 cut(s) 375
Hin1II CATG 3 cut(s) 281, 508, 518
HpaII CCGG 1 cut(s) 375
Hpy166II GTNNAC 1 cut(s) 392
Hpy188III TCNNGA 4 cut(s) 32, 85, 311, 458
Hpy8I GTNNAC 1 cut(s) 392
HpyCH4III ACNGT 1 cut(s) 51
HpyCH4IV ACGT 1 cut(s) 404
HpyCH4V TGCA 2 cut(s) 64, 98
HpyF10VI GCNNNNNNNGC 2 cut(s) 43, 283
HpyF3I CTNAG 1 cut(s) 459
HpySE526I ACGT 1 cut(s) 404
Hsp92II CATG 3 cut(s) 281, 508, 518
Kzo9I GATC 3 cut(s) 107, 280, 307
LmnI GCTCC 1 cut(s) 460
LpnPI CCDG 7 cut(s) 98, 128, 198, 333, 372, 388, 471
Lsp1109I GCAGC 1 cut(s) 229
LweI GCATC 4 cut(s) 85, 305, 316, 463
MaeI CTAG 1 cut(s) 311
MaeII ACGT 1 cut(s) 404
MaeIII GTNAC 1 cut(s) 400
MalI GATC 3 cut(s) 109, 282, 309
MbiI CCGCTC 1 cut(s) 37
MboI GATC 3 cut(s) 107, 280, 307
MboII GAAGA 7 cut(s) 158, 263, 274, 291, 335, 424, 482
MfeI CAATTG 1 cut(s) 303
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 7 cut(s) 16, 202, 237, 303, 331, 433, 491
MnlI CCTC 3 cut(s) 68, 402, 454
Mph1103I ATGCAT 1 cut(s) 100
MseI TTAA 2 cut(s) 189, 351
MslI CAYNNNNRTG 1 cut(s) 230
MspI CCGG 1 cut(s) 375
MspR9I CCNGG 1 cut(s) 376
MunI CAATTG 1 cut(s) 303
MwoI GCNNNNNNNGC 2 cut(s) 43, 283
NciI CCSGG 1 cut(s) 376
NdeII GATC 3 cut(s) 107, 280, 307
NlaIII CATG 3 cut(s) 281, 508, 518
NsiI ATGCAT 1 cut(s) 100
PkrI GCNGC 2 cut(s) 39, 244
Psp1406I AACGTT 1 cut(s) 404
PspPI GGNCC 1 cut(s) 317
RsaI GTAC 1 cut(s) 431
RsaNI GTAC 1 cut(s) 430
RseI CAYNNNNRTG 1 cut(s) 230
SaqAI TTAA 2 cut(s) 189, 351
SatI GCNGC 2 cut(s) 38, 243
Sau3AI GATC 3 cut(s) 107, 280, 307
Sau96I GGNCC 1 cut(s) 317
ScrFI CCNGG 1 cut(s) 376
SduI GDGCHC 1 cut(s) 186
SetI ASST 4 cut(s) 48, 407, 465, 486
SfaNI GCATC 4 cut(s) 85, 305, 316, 463
SfcI CTRYAG 1 cut(s) 47
SmiMI CAYNNNNRTG 1 cut(s) 230
Sse9I AATT 7 cut(s) 16, 202, 237, 303, 331, 433, 491
SsiI CCGC 1 cut(s) 37
SspMI CTAG 1 cut(s) 311
StyD4I CCNGG 1 cut(s) 374
TaaI ACNGT 1 cut(s) 51
TaiI ACGT 1 cut(s) 407
TaqI TCGA 1 cut(s) 106
TaqII GACCGA 1 cut(s) 23
TasI AATT 7 cut(s) 16, 202, 237, 303, 331, 433, 491
TauI GCSGC 1 cut(s) 40
Tru1I TTAA 2 cut(s) 189, 351
Tru9I TTAA 2 cut(s) 189, 351
TscAI CASTG 1 cut(s) 217
TseI GCWGC 1 cut(s) 242
TspRI CASTG 1 cut(s) 217
XapI RAATTY 1 cut(s) 491
XbaI TCTAGA 1 cut(s) 310
XmiI GTMKAC 1 cut(s) 391
XspI CTAG 1 cut(s) 311
Zsp2I ATGCAT 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.