RLG00000036006

Josephin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0015189
Physical Location & Seq
Forward (+)
74796552 .. 74797264
713 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036006

Sequence Viewer

Length: 552 bp
ATGGCGGGCGAGAACAATACCCAAATCTACCACGAGCGCCAGAAGCTTCAGTTTTGCCTCTTACACGCGCTCAACAATCTCTTCCAGCAAAAAGATGCGTTTACTCGACCGAGATTGAATGAGATTGCTGCAAAACTTGTTCTTGAAGAACCCAACAAGGAAACATGGACACCCTTATCTCTGCTCTTTAAACCCCATCATAATGCACTTACCGGAAACTATGACATAAATGTGCTAATTGCTGCTTTAGAAGATAGGGGGAAGAATGTGGTTTGGCACGATCGTCGGAAGGGGGCCAATTCGATTGATCTAGATGGGGCCGAAGATGCTTTGATGGGTATTCTGCTTAATGTTCCGGTTAAAAGGTTTGCGGGGCTATGGAAAAGTAGGCATTGGATTACGTTGAGGACGATTGATGGGGTTTGGTACGATTTGGATAGTGATCTTGATGCTCCTCAGGCTTTTGAAGATAGTGGAAGAGTTAGAGAGTTCTTGGATTACATCATTGGGCATGGTGGAGAGGTTTTGCTTGTTCTGAATAATAAACAGTGA

Protein Analysis

184

Amino Acids

20.95

Weight (kDa)

5.96

Isoelectric Point (pI)

39.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Josephin PF02099 14 - 178 2.5e-36 Josephin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015189)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29640
fragaria_vesca FvH4_3g38250 FvH4_3g38250
malus_domestica MD11G1079900.v1.1
prunus_persica Prupe.6G060500_v2.0.a1
pyrus_communis pycom11g06740
rosa_chinensis RchiOBHm_Chr5g0068731
rosa_laevigata RLG00000036006
rosa_multiflora Rmu_sc0000376.1_g000026
rosa_roxburghii Rroxscaffold_1G00012090
rosa_rugosa Rorug05G0392700
rosa_samantha Rh5AG450200 Rh5BG468600 Rh5CG490800 Rh5DG482100
rosa_wichuraiana Rw5G042040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 68
AciI CCGC 2 cut(s) 5, 371
AcuI CTGAAG 1 cut(s) 32
AfaI GTAC 1 cut(s) 428
AgsI TTSAA 3 cut(s) 118, 146, 467
AluBI AGCT 1 cut(s) 46
AluI AGCT 1 cut(s) 46
AoxI GGCC 2 cut(s) 294, 318
ApeKI GCWGC 2 cut(s) 128, 242
AspLEI GCGC 2 cut(s) 39, 70
AspS9I GGNCC 2 cut(s) 294, 318
AxyI CCTNAGG 1 cut(s) 456
BauI CACGAG 1 cut(s) 32
BbvI GCAGC 2 cut(s) 115, 229
BccI CCATC 4 cut(s) 204, 308, 328, 410
BcgI CGANNNNNNTGC 2 cut(s) 266, 300
BfaI CTAG 1 cut(s) 311
BfoI RGCGCY 1 cut(s) 40
BisI GCNGC 2 cut(s) 129, 243
BlsI GCNGC 2 cut(s) 130, 244
BmgT120I GGNCC 2 cut(s) 294, 318
BmiI GGNNCC 2 cut(s) 295, 319
BmsI GCATC 3 cut(s) 85, 316, 439
BsaBI GATNNNNATC 1 cut(s) 441
BsaWI WCCGGW 2 cut(s) 212, 355
Bse21I CCTNAGG 1 cut(s) 456
Bse8I GATNNNNATC 1 cut(s) 441
BseJI GATNNNNATC 1 cut(s) 441
BseMII CTCAG 1 cut(s) 470
BseRI GAGGAG 1 cut(s) 444
BseXI GCAGC 2 cut(s) 115, 229
Bsh1236I CGCG 1 cut(s) 68
Bsh1285I CGRYCG 2 cut(s) 110, 283
BshFI GGCC 2 cut(s) 296, 320
BsiEI CGRYCG 2 cut(s) 110, 283
BsiSI CCGG 2 cut(s) 213, 356
BsnI GGCC 2 cut(s) 296, 320
Bsp143I GATC 3 cut(s) 280, 307, 442
BspACI CCGC 2 cut(s) 5, 371
BspANI GGCC 2 cut(s) 296, 320
BspCNI CTCAG 1 cut(s) 469
BspFNI CGCG 1 cut(s) 68
BspLI GGNNCC 2 cut(s) 295, 319
BssMI GATC 3 cut(s) 280, 307, 442
BssSI CACGAG 1 cut(s) 32
Bst2BI CACGAG 1 cut(s) 32
Bst4CI ACNGT 1 cut(s) 549
Bst6I CTCTTC 2 cut(s) 86, 472
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 1 cut(s) 456
BstFNI CGCG 1 cut(s) 68
BstH2I RGCGCY 1 cut(s) 40
BstHHI GCGC 2 cut(s) 39, 70
BstKTI GATC 3 cut(s) 283, 310, 445
BstMBI GATC 3 cut(s) 280, 307, 442
BstMCI CGRYCG 2 cut(s) 110, 283
BstMWI GCNNNNNNNGC 3 cut(s) 43, 326, 458
BstUI CGCG 1 cut(s) 68
BstV1I GCAGC 2 cut(s) 115, 229
Bsu36I CCTNAGG 1 cut(s) 456
BsuRI GGCC 2 cut(s) 296, 320
Cac8I GCNNGC 1 cut(s) 7
CfoI GCGC 2 cut(s) 39, 70
Cfr13I GGNCC 2 cut(s) 294, 318
Csp6I GTAC 1 cut(s) 427
CviAII CATG 2 cut(s) 165, 512
CviJI RGCY 5 cut(s) 46, 296, 320, 376, 461
CviKI_1 RGCY 5 cut(s) 46, 296, 320, 376, 461
CviQI GTAC 1 cut(s) 427
DdeI CTNAG 1 cut(s) 456
DpnI GATC 3 cut(s) 282, 309, 444
DpnII GATC 3 cut(s) 280, 307, 442
DraI TTTAAA 1 cut(s) 190
Eam1104I CTCTTC 2 cut(s) 86, 472
EarI CTCTTC 2 cut(s) 86, 472
Eco57I CTGAAG 1 cut(s) 32
Eco81I CCTNAGG 1 cut(s) 456
FaeI CATG 2 cut(s) 168, 515
FaiI YATR 6 cut(s) 166, 201, 222, 227, 379, 513
FatI CATG 2 cut(s) 164, 511
FauI CCCGC 1 cut(s) 364
Fnu4HI GCNGC 2 cut(s) 129, 243
Fsp4HI GCNGC 2 cut(s) 129, 243
FspBI CTAG 1 cut(s) 311
GlaI GCGC 2 cut(s) 38, 69
GluI GCNGC 2 cut(s) 129, 243
HaeII RGCGCY 1 cut(s) 40
HaeIII GGCC 2 cut(s) 296, 320
HapII CCGG 2 cut(s) 213, 356
HhaI GCGC 2 cut(s) 39, 70
Hin1II CATG 2 cut(s) 168, 515
Hin6I GCGC 2 cut(s) 37, 68
HinP1I GCGC 2 cut(s) 37, 68
HindIII AAGCTT 1 cut(s) 44
HpaII CCGG 2 cut(s) 213, 356
Hpy166II GTNNAC 1 cut(s) 102
Hpy188I TCNGA 2 cut(s) 288, 537
Hpy188III TCNNGA 3 cut(s) 143, 311, 446
Hpy8I GTNNAC 1 cut(s) 102
Hpy99I CGWCG 1 cut(s) 288
HpyAV CCTTC 1 cut(s) 283
HpyCH4III ACNGT 1 cut(s) 549
HpyCH4IV ACGT 1 cut(s) 401
HpyCH4V TGCA 2 cut(s) 131, 206
HpyF10VI GCNNNNNNNGC 3 cut(s) 43, 326, 458
HpyF3I CTNAG 1 cut(s) 456
HpySE526I ACGT 1 cut(s) 401
Hsp92II CATG 2 cut(s) 168, 515
HspAI GCGC 2 cut(s) 37, 68
Kzo9I GATC 3 cut(s) 280, 307, 442
LmnI GCTCC 1 cut(s) 457
LpnPI CCDG 5 cut(s) 53, 98, 226, 369, 443
Lsp1109I GCAGC 2 cut(s) 115, 229
LweI GCATC 3 cut(s) 85, 316, 439
MaeI CTAG 1 cut(s) 311
MaeII ACGT 1 cut(s) 401
MalI GATC 3 cut(s) 282, 309, 444
MboI GATC 3 cut(s) 280, 307, 442
MboII GAAGA 7 cut(s) 73, 158, 263, 274, 335, 479, 489
MluCI AATT 2 cut(s) 237, 298
MmeI TCCRAC 1 cut(s) 266
MnlI CCTC 4 cut(s) 68, 399, 465, 514
MseI TTAA 3 cut(s) 189, 348, 360
MslI CAYNNNNRTG 2 cut(s) 201, 230
MspI CCGG 2 cut(s) 213, 356
MvnI CGCG 1 cut(s) 68
MwoI GCNNNNNNNGC 3 cut(s) 43, 326, 458
NdeII GATC 3 cut(s) 280, 307, 442
NlaIII CATG 2 cut(s) 168, 515
NlaIV GGNNCC 2 cut(s) 295, 319
PcsI WCGNNNNNNNCGW 1 cut(s) 407
PkrI GCNGC 2 cut(s) 130, 244
Ple19I CGATCG 1 cut(s) 283
PspN4I GGNNCC 2 cut(s) 295, 319
PspPI GGNCC 2 cut(s) 294, 318
PvuI CGATCG 1 cut(s) 283
RsaI GTAC 1 cut(s) 428
RsaNI GTAC 1 cut(s) 427
RseI CAYNNNNRTG 2 cut(s) 201, 230
SaqAI TTAA 3 cut(s) 189, 348, 360
SatI GCNGC 2 cut(s) 129, 243
Sau3AI GATC 3 cut(s) 280, 307, 442
Sau96I GGNCC 2 cut(s) 294, 318
SetI ASST 4 cut(s) 48, 368, 404, 525
SfaNI GCATC 3 cut(s) 85, 316, 439
SmiMI CAYNNNNRTG 2 cut(s) 201, 230
Sse9I AATT 2 cut(s) 237, 298
SsiI CCGC 2 cut(s) 5, 371
SspMI CTAG 1 cut(s) 311
TaaI ACNGT 1 cut(s) 549
TaiI ACGT 1 cut(s) 404
TaqI TCGA 2 cut(s) 106, 302
TaqII GACCGA 1 cut(s) 124
TasI AATT 2 cut(s) 237, 298
Tru1I TTAA 3 cut(s) 189, 348, 360
Tru9I TTAA 3 cut(s) 189, 348, 360
TseI GCWGC 2 cut(s) 128, 242
XbaI TCTAGA 1 cut(s) 310
XspI CTAG 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.