MD11G1298600.v1.1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0021208
Physical Location & Seq
Reverse (-)
41525452 .. 41527029
1578 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1298600.v1.1.491

Sequence Viewer

Length: 1416 bp
ATGGCAGTACTGGCTTTAGTTTACAAACTGTCAACTAGTTTGTTCTTGCTGCTGTCACTACTGTGGGGTGCTGCCATTGCTGTATCATCCAAACCAAATGGCTTTAGCGTCGAACTCATGCACATCTTTTCACCAGCATCGCCTCTTTACCCCGGCAACATTTCCTTCCAAGAAGAAATGCAACGACTCTTACAGCGATCGGATGTGCGAATGCGGCACATCGATGCTACCATTGCATCAGCCCTAACCCTAAGCAATAACATGTCACAAACTGCAAAGAACCAGCTTGATGATGAGTTTATTCGTCCGCAAGTCGAATATTACCCTATCGCCTCTTACATTGTAGCAGTAGGCGTTGGCACTTTCCCAGACGCGAAGTTCCCGGAGAGACCATTCAAATCATACTACTTGTACATGGACACTGGGAGCAGCCTCACCTGGGTACTCTGCGCGGATTGCGAAAAGCAGAAGCCTCCGAGATGCTTTAAAACCAGGGAACCCCCTTTCCCTAACGGCGAATCCCAATCTTATAAGCCTCTCCCTTGCAACAAACACCGCTTTTGCACACCAAACCTGTGTATTGGCGATTACTGCTCCGAAGCCACAGAAGAAACTTTCACGTTTTTGTCAAACAAAGGGCGGCCCCTGCCTATCGAAGACATAGTGTTTGGTTGTGCACATGACACAAGGAACGTAGACTTTGGCAGCCAGGAAGACTTCAAGGTAGCCGGATTTATGGGTTTGGGATATTCTCCTTCTTCATTTCCATTGCAAATATCCCATCTTATTCAAGGAAAATTCTCCTTCTGCCTATCACACAACCGAGAGATTCCAACATATCTCAGATTTGGCGGAGACATACCCGAACCATCCAGCGGCCTACGCGTCACGAATTTAGTCCTCATTCCAGAGATACCCTATTACTACGTGAACTTGAAAGCCATAAGTGTGAATGGACAGAAGCTCCTGATCGATCCATCGGTGTTTGCTCTACGATCCGGGGGTTATCAAGGAGGCTGCCTCATGGACAATGGCAGTTCAGTTACTTATCTCATCACCCCAGCTTATAAGGCATTGGTAAAATTCCTGGAAATGTTTTATATGAGATATCCAAACTATGTTAGGCATCTTGGCCCCATTAATCCCGCGTTCGAGCTTTGTTATAGATGGGCGCCGCCGATCGTTCCCCTCCCTGCTGTTACATTTCACTTTGAGGGTGCTGACCTTGAGTTGAAAGACGAGGCGTTCATAACGCTGGAGGATCGAGATGGGAGCAGTATTTTGTGTTTGGCGATGATGGAAAATACAGTTCGTACTATAGTTGGATCATTGCAGCAAACAAATTACAGATTTATATATGACCTTAACAATGCGCTGCTCAAGTTTGTTCCTGAGGATTGTGCCAAGTGGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

472

Amino Acids

52.98

Weight (kDa)

5.96

Isoelectric Point (pI)

47.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 131 - 189 2.4e-09 Xylanase inhibitor N-terminal
Asp PF00026 131 - 462 2.9e-08 Eukaryotic aspartyl protease
TAXi_N PF14543 201 - 285 3.2e-13 Xylanase inhibitor N-terminal
TAXi_C PF14541 308 - 463 1.4e-23 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021208)

Species Orthologous Gene IDs
malus_domestica MD11G1298600.v1.1
prunus_persica Prupe.3G234600_v2.0.a1 Prupe.8G253600_v2.0.a1 Prupe.8G253700_v2.0.a1 Prupe.8G253800_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 531, 1068
AccB1I GGYRCC 1 cut(s) 1171
AccI GTMKAC 1 cut(s) 696
AccII CGCG 4 cut(s) 374, 452, 885, 1148
AciI CCGC 9 cut(s) 214, 308, 452, 556, 640, 852, 876, 1146, 1175
AclWI GGATC 4 cut(s) 968, 990, 1269, 1333
AcsI RAATTY 3 cut(s) 797, 892, 1082
AcyI GRCGYC 1 cut(s) 1172
AfaI GTAC 4 cut(s) 9, 413, 444, 1315
AfiI CCNNNNNNNGG 2 cut(s) 439, 875
AflIII ACRYGT 2 cut(s) 261, 883
AgsI TTSAA 5 cut(s) 397, 721, 791, 937, 1234
AhlI ACTAGT 1 cut(s) 35
AjnI CCWGG 4 cut(s) 437, 491, 708, 1086
AleI CACNNNNGTG 1 cut(s) 61
AloI GAACNNNNNNTCC 2 cut(s) 489, 521
AluBI AGCT 4 cut(s) 286, 964, 1064, 1156
AluI AGCT 4 cut(s) 286, 964, 1064, 1156
Alw21I GWGCWC 1 cut(s) 679
Alw26I GTCTC 2 cut(s) 382, 849
Alw44I GTGCAC 1 cut(s) 675
AlwI GGATC 4 cut(s) 968, 990, 1269, 1333
AoxI GGCC 3 cut(s) 641, 877, 1132
ApaLI GTGCAC 1 cut(s) 675
ApeKI GCWGC 7 cut(s) 49, 71, 429, 705, 1017, 1333, 1375
ApoI RAATTY 3 cut(s) 797, 892, 1082
ArsI GACNNNNNNTTYG 2 cut(s) 650, 682
AseI ATTAAT 1 cut(s) 1140
AspLEI GCGC 3 cut(s) 452, 1174, 1375
AspS9I GGNCC 2 cut(s) 642, 1133
AsuC2I CCSGG 3 cut(s) 153, 383, 1000
AsuHPI GGTGA 3 cut(s) 123, 427, 1048
AxyI CCTNAGG 1 cut(s) 1392
BaeGI GKGCMC 1 cut(s) 679
BanI GGYRCC 1 cut(s) 1171
BbsI GAAGAC 2 cut(s) 663, 720
Bbv12I GWGCWC 1 cut(s) 679
BbvI GCAGC 7 cut(s) 36, 58, 441, 717, 1004, 1345, 1362
BccI CCATC 6 cut(s) 789, 877, 985, 1161, 1262, 1291
BceAI ACGGC 1 cut(s) 529
BciT130I CCWGG 4 cut(s) 439, 493, 710, 1088
BcnI CCSGG 3 cut(s) 153, 383, 1000
BcoDI GTCTC 2 cut(s) 382, 849
BcuI ACTAGT 1 cut(s) 35
BfaI CTAG 1 cut(s) 36
BfmI CTRYAG 1 cut(s) 1317
BfoI RGCGCY 1 cut(s) 1175
BmcAI AGTACT 1 cut(s) 9
Bme1390I CCNGG 7 cut(s) 153, 383, 439, 493, 710, 1000, 1088
BmgT120I GGNCC 2 cut(s) 642, 1133
BmiI GGNNCC 4 cut(s) 498, 644, 1135, 1173
BmrFI CCNGG 7 cut(s) 153, 383, 439, 493, 710, 1000, 1088
BmrI ACTGGG 1 cut(s) 432
BmsI GCATC 5 cut(s) 146, 214, 245, 470, 1135
BmuI ACTGGG 1 cut(s) 432
BpiI GAAGAC 2 cut(s) 663, 720
BplI GAGNNNNNCTC 2 cut(s) 1005, 1037
BpmI CTGGAG 1 cut(s) 1277
Bpu10I CCTNAGC 1 cut(s) 251
BpuEI CTTGAG 2 cut(s) 1247, 1364
BpuMI CCSGG 3 cut(s) 153, 383, 1000
Bsa29I ATCGAT 2 cut(s) 222, 972
BsaAI YACGTR 1 cut(s) 928
BsaHI GRCGYC 1 cut(s) 1172
BsaI GGTCTC 1 cut(s) 382
BsaJI CCNNGG 4 cut(s) 151, 438, 492, 999
BsaXI ACNNNNNCTCC 2 cut(s) 948, 978
Bsc4I CCNNNNNNNGG 2 cut(s) 439, 875
Bse1I ACTGG 2 cut(s) 15, 427
Bse21I CCTNAGG 1 cut(s) 1392
Bse3DI GCAATG 4 cut(s) 75, 231, 767, 1328
BseBI CCWGG 4 cut(s) 439, 493, 710, 1088
BseCI ATCGAT 2 cut(s) 222, 972
BseDI CCNNGG 4 cut(s) 151, 438, 492, 999
BseGI GGATG 3 cut(s) 86, 208, 869
BseLI CCNNNNNNNGG 2 cut(s) 439, 875
BseMI GCAATG 4 cut(s) 75, 231, 767, 1328
BseMII CTCAG 2 cut(s) 856, 1383
BseNI ACTGG 2 cut(s) 15, 427
BseSI GKGCMC 1 cut(s) 679
BseXI GCAGC 7 cut(s) 36, 58, 441, 717, 1004, 1345, 1362
BseYI CCCAGC 1 cut(s) 1060
Bsh1236I CGCG 4 cut(s) 374, 452, 885, 1148
Bsh1285I CGRYCG 2 cut(s) 200, 1182
BshFI GGCC 3 cut(s) 643, 879, 1134
BshNI GGYRCC 1 cut(s) 1171
BshVI ATCGAT 2 cut(s) 222, 972
BsiEI CGRYCG 2 cut(s) 200, 1182
BsiHKAI GWGCWC 1 cut(s) 679
BsiSI CCGG 4 cut(s) 153, 383, 729, 999
BslI CCNNNNNNNGG 2 cut(s) 439, 875
BsmAI GTCTC 2 cut(s) 382, 849
BsmI GAATGC 1 cut(s) 216
BsnI GGCC 3 cut(s) 643, 879, 1134
Bso31I GGTCTC 1 cut(s) 382
Bsp1286I GDGCHC 1 cut(s) 679
Bsp1407I TGTACA 1 cut(s) 411
Bsp143I GATC 7 cut(s) 197, 969, 973, 995, 1179, 1261, 1325
BspACI CCGC 9 cut(s) 214, 308, 452, 556, 640, 852, 876, 1146, 1175
BspANI GGCC 3 cut(s) 643, 879, 1134
BspCNI CTCAG 2 cut(s) 855, 1384
BspDI ATCGAT 2 cut(s) 222, 972
BspFNI CGCG 4 cut(s) 374, 452, 885, 1148
BspLI GGNNCC 4 cut(s) 498, 644, 1135, 1173
BspPI GGATC 4 cut(s) 968, 990, 1269, 1333
BspT107I GGYRCC 1 cut(s) 1171
BspTNI GGTCTC 1 cut(s) 382
BsrDI GCAATG 4 cut(s) 75, 231, 767, 1328
BsrGI TGTACA 1 cut(s) 411
BsrI ACTGG 2 cut(s) 15, 427
BssECI CCNNGG 4 cut(s) 151, 438, 492, 999
BssMI GATC 7 cut(s) 197, 969, 973, 995, 1179, 1261, 1325
BssNI GRCGYC 1 cut(s) 1172
Bst2UI CCWGG 4 cut(s) 439, 493, 710, 1088
Bst4CI ACNGT 3 cut(s) 30, 63, 1309
BstACI GRCGYC 1 cut(s) 1172
BstAUI TGTACA 1 cut(s) 411
BstBAI YACGTR 1 cut(s) 928
BstDEI CTNAG 3 cut(s) 251, 842, 1392
BstF5I GGATG 3 cut(s) 86, 208, 869
BstFNI CGCG 4 cut(s) 374, 452, 885, 1148
BstH2I RGCGCY 1 cut(s) 1175
BstHHI GCGC 3 cut(s) 452, 1174, 1375
BstKTI GATC 7 cut(s) 200, 972, 976, 998, 1182, 1264, 1328
BstMAI GTCTC 2 cut(s) 382, 849
BstMBI GATC 7 cut(s) 197, 969, 973, 995, 1179, 1261, 1325
BstMCI CGRYCG 2 cut(s) 200, 1182
BstMWI GCNNNNNNNGC 9 cut(s) 11, 77, 214, 233, 456, 591, 646, 882, 1070
BstNI CCWGG 4 cut(s) 439, 493, 710, 1088
BstNSI RCATGY 1 cut(s) 265
BstSCI CCNGG 7 cut(s) 151, 381, 437, 491, 708, 998, 1086
BstSFI CTRYAG 1 cut(s) 1317
BstSLI GKGCMC 1 cut(s) 679
BstUI CGCG 4 cut(s) 374, 452, 885, 1148
BstV1I GCAGC 7 cut(s) 36, 58, 441, 717, 1004, 1345, 1362
BstV2I GAAGAC 2 cut(s) 663, 720
Bsu15I ATCGAT 2 cut(s) 222, 972
Bsu36I CCTNAGG 1 cut(s) 1392
BsuRI GGCC 3 cut(s) 643, 879, 1134
BsuTUI ATCGAT 2 cut(s) 222, 972
BtgZI GCGATG 2 cut(s) 123, 1307
BtsCI GGATG 3 cut(s) 86, 208, 869
BtsIMutI CAGTG 1 cut(s) 420
CfoI GCGC 3 cut(s) 452, 1174, 1375
Cfr13I GGNCC 2 cut(s) 642, 1133
ClaI ATCGAT 2 cut(s) 222, 972
CseI GACGC 3 cut(s) 97, 380, 874
Csp6I GTAC 4 cut(s) 8, 412, 443, 1314
CviAII CATG 5 cut(s) 118, 262, 415, 680, 1024
CviQI GTAC 4 cut(s) 8, 412, 443, 1314
DdeI CTNAG 3 cut(s) 251, 842, 1392
DinI GGCGCC 1 cut(s) 1173
DpnI GATC 7 cut(s) 199, 971, 975, 997, 1181, 1263, 1327
DpnII GATC 7 cut(s) 197, 969, 973, 995, 1179, 1261, 1325
DraI TTTAAA 1 cut(s) 487
EciI GGCGGA 1 cut(s) 867
Eco31I GGTCTC 1 cut(s) 382
Eco32I GATATC 1 cut(s) 1109
Eco81I CCTNAGG 1 cut(s) 1392
EcoRII CCWGG 4 cut(s) 437, 491, 708, 1086
EcoRV GATATC 1 cut(s) 1109
EgeI GGCGCC 1 cut(s) 1173
EheI GGCGCC 1 cut(s) 1173
FaeI CATG 5 cut(s) 121, 265, 418, 683, 1027
FatI CATG 5 cut(s) 117, 261, 414, 679, 1023
FauI CCCGC 1 cut(s) 1153
FblI GTMKAC 1 cut(s) 696
FokI GGATG 3 cut(s) 73, 215, 856
FspBI CTAG 1 cut(s) 36
GlaI GCGC 3 cut(s) 451, 1173, 1374
GsaI CCCAGC 1 cut(s) 1064
GsuI CTGGAG 1 cut(s) 1277
HaeII RGCGCY 1 cut(s) 1175
HaeIII GGCC 3 cut(s) 643, 879, 1134
HapII CCGG 4 cut(s) 153, 383, 729, 999
HgaI GACGC 3 cut(s) 97, 380, 874
HhaI GCGC 3 cut(s) 452, 1174, 1375
Hin1I GRCGYC 1 cut(s) 1172
Hin1II CATG 5 cut(s) 121, 265, 418, 683, 1027
Hin6I GCGC 3 cut(s) 450, 1172, 1373
HinP1I GCGC 3 cut(s) 450, 1172, 1373
HincII GTYRAC 1 cut(s) 33
HindII GTYRAC 1 cut(s) 33
HinfI GANTC 3 cut(s) 186, 518, 829
HpaII CCGG 4 cut(s) 153, 383, 729, 999
HphI GGTGA 3 cut(s) 123, 427, 1048
Hpy166II GTNNAC 5 cut(s) 22, 33, 677, 697, 931
Hpy188I TCNGA 4 cut(s) 202, 477, 598, 845
Hpy188III TCNNGA 6 cut(s) 889, 908, 967, 1265, 1391, 1413
Hpy8I GTNNAC 5 cut(s) 22, 33, 677, 697, 931
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 3 cut(s) 175, 765, 814
HpyCH4III ACNGT 3 cut(s) 30, 63, 1309
HpyCH4IV ACGT 3 cut(s) 620, 693, 927
HpyCH4V TGCA 9 cut(s) 121, 181, 236, 275, 546, 564, 677, 772, 1333
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 77, 214, 233, 456, 591, 646, 882, 1070
HpyF3I CTNAG 3 cut(s) 251, 842, 1392
HpySE526I ACGT 3 cut(s) 620, 693, 927
Hsp92I GRCGYC 1 cut(s) 1172
Hsp92II CATG 5 cut(s) 121, 265, 418, 683, 1027
HspAI GCGC 3 cut(s) 450, 1172, 1373
KasI GGCGCC 1 cut(s) 1171
Kzo9I GATC 7 cut(s) 197, 969, 973, 995, 1179, 1261, 1325
LmnI GCTCC 4 cut(s) 426, 599, 969, 1272
Lsp1109I GCAGC 7 cut(s) 36, 58, 441, 717, 1004, 1345, 1362
LweI GCATC 5 cut(s) 146, 214, 245, 470, 1135
MaeI CTAG 1 cut(s) 36
MaeII ACGT 3 cut(s) 620, 693, 927
MaeIII GTNAC 5 cut(s) 54, 264, 886, 1042, 1198
MalI GATC 7 cut(s) 199, 971, 975, 997, 1181, 1263, 1327
MboI GATC 7 cut(s) 197, 969, 973, 995, 1179, 1261, 1325
MboII GAAGA 5 cut(s) 185, 620, 668, 725, 750
MhlI GDGCHC 1 cut(s) 679
MluCI AATT 4 cut(s) 797, 892, 1082, 1342
MluI ACGCGT 1 cut(s) 883
Mly113I GGCGCC 1 cut(s) 1172
MlyI GAGTC 1 cut(s) 180
MmeI TCCRAC 2 cut(s) 857, 1303
MseI TTAA 3 cut(s) 486, 1140, 1365
MslI CAYNNNNRTG 3 cut(s) 61, 222, 947
MspA1I CMGCKG 1 cut(s) 876
MspI CCGG 4 cut(s) 153, 383, 729, 999
MspR9I CCNGG 7 cut(s) 153, 383, 439, 493, 710, 1000, 1088
Mva1269I GAATGC 1 cut(s) 216
MvaI CCWGG 4 cut(s) 439, 493, 710, 1088
MvnI CGCG 4 cut(s) 374, 452, 885, 1148
MwoI GCNNNNNNNGC 9 cut(s) 11, 77, 214, 233, 456, 591, 646, 882, 1070
NarI GGCGCC 1 cut(s) 1172
NciI CCSGG 3 cut(s) 153, 383, 1000
NdeII GATC 7 cut(s) 197, 969, 973, 995, 1179, 1261, 1325
NlaIII CATG 5 cut(s) 121, 265, 418, 683, 1027
NlaIV GGNNCC 4 cut(s) 498, 644, 1135, 1173
NmuCI GTSAC 3 cut(s) 54, 264, 886
NspI RCATGY 1 cut(s) 265
OliI CACNNNNGTG 1 cut(s) 61
PciI ACATGT 1 cut(s) 261
PctI GAATGC 1 cut(s) 216
PfeI GAWTC 2 cut(s) 518, 829
PfoI TCCNGGA 2 cut(s) 381, 1086
Ple19I CGATCG 2 cut(s) 200, 1182
PleI GAGTC 1 cut(s) 180
PluTI GGCGCC 1 cut(s) 1175
PpsI GAGTC 1 cut(s) 180
Ppu21I YACGTR 1 cut(s) 928
PscI ACATGT 1 cut(s) 261
PshBI ATTAAT 1 cut(s) 1140
PsiI TTATAA 2 cut(s) 531, 1068
Psp6I CCWGG 4 cut(s) 437, 491, 708, 1086
PspFI CCCAGC 1 cut(s) 1060
PspGI CCWGG 4 cut(s) 437, 491, 708, 1086
PspN4I GGNNCC 4 cut(s) 498, 644, 1135, 1173
PspPI GGNCC 2 cut(s) 642, 1133
PvuI CGATCG 2 cut(s) 200, 1182
RsaI GTAC 4 cut(s) 9, 413, 444, 1315
RsaNI GTAC 4 cut(s) 8, 412, 443, 1314
RseI CAYNNNNRTG 3 cut(s) 61, 222, 947
SaqAI TTAA 3 cut(s) 486, 1140, 1365
Sau3AI GATC 7 cut(s) 197, 969, 973, 995, 1179, 1261, 1325
Sau96I GGNCC 2 cut(s) 642, 1133
ScaI AGTACT 1 cut(s) 9
SchI GAGTC 1 cut(s) 180
ScrFI CCNGG 7 cut(s) 153, 383, 439, 493, 710, 1000, 1088
SduI GDGCHC 1 cut(s) 679
SfaNI GCATC 5 cut(s) 146, 214, 245, 470, 1135
SfcI CTRYAG 1 cut(s) 1317
SfoI GGCGCC 1 cut(s) 1173
SmiMI CAYNNNNRTG 3 cut(s) 61, 222, 947
SmlI CTYRAG 2 cut(s) 1226, 1379
SmoI CTYRAG 2 cut(s) 1226, 1379
SpeI ACTAGT 1 cut(s) 35
Sse9I AATT 4 cut(s) 797, 892, 1082, 1342
SsiI CCGC 9 cut(s) 214, 308, 452, 556, 640, 852, 876, 1146, 1175
SspDI GGCGCC 1 cut(s) 1171
SspI AATATT 1 cut(s) 320
SspMI CTAG 1 cut(s) 36
StyD4I CCNGG 7 cut(s) 151, 381, 437, 491, 708, 998, 1086
TaaI ACNGT 3 cut(s) 30, 63, 1309
TaiI ACGT 3 cut(s) 623, 696, 930
TaqI TCGA 7 cut(s) 111, 222, 315, 654, 972, 1152, 1264
TasI AATT 4 cut(s) 797, 892, 1082, 1342
TatI WGTACW 2 cut(s) 7, 411
TauI GCSGC 4 cut(s) 217, 643, 879, 1177
TfiI GAWTC 2 cut(s) 518, 829
Tru1I TTAA 3 cut(s) 486, 1140, 1365
Tru9I TTAA 3 cut(s) 486, 1140, 1365
TscAI CASTG 1 cut(s) 427
TseFI GTSAC 3 cut(s) 54, 264, 886
TseI GCWGC 7 cut(s) 49, 71, 429, 705, 1017, 1333, 1375
Tsp45I GTSAC 3 cut(s) 54, 264, 886
TspDTI ATGAA 2 cut(s) 750, 1237
TspRI CASTG 1 cut(s) 427
VneI GTGCAC 1 cut(s) 675
VspI ATTAAT 1 cut(s) 1140
XapI RAATTY 3 cut(s) 797, 892, 1082
XceI RCATGY 1 cut(s) 265
XmiI GTMKAC 1 cut(s) 696
XspI CTAG 1 cut(s) 36
ZrmI AGTACT 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.