MD12G1028000.v1.1
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010872
Physical Location & Seq
Forward (+)
3130306 .. 3137111
6806 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1028000.v1.1.491

Sequence Viewer

Length: 3030 bp
ATGTCCGGCCGCCACGAGAAAGAGAAGGGCGTTAACGTCCAGGTCCTTCTCCGTTGCAGGCCGTTTAGTGAGGATGAGTTGCGGAGCAATGCCCCGCAGGTTATTACTTGTAATGAGTACCAGAGGGAAGTGGCCGTGTCTCAGAACATTGCCGGAAAACACATCGATAGGGTTTTCACTTTCGATAAGGTTTTTGGTCCTAATGCTCAGCAAAGAGATCTTTATGATCAAGCTGTGATTCCAATAGTGCACGAAGTCTTGGAAGGCTTCAACTGTACCATTTTTGCGTATGGGCAAACTGGTACCGGTAAGACATACACAATGGAGGGTGAATTCAAAAGGGCAAAGACTGGGCCTAATGGAGAATTGCCTCCCGAGGCTGGAGTTATTCCAAGGGCTGTCCAGCAGATTTTCGATACCCTTGAGGGCCAGAATGCAGAGTACAGTGTGAAAGTCACTTTCTTAGAGCTTTACAACGAAGAGATTACTGATCTTCTTGCTCCAGAGGAACTTAATAGAGTTTCTTTGGAAGATAAGCAGAAAAAGCAGCTGCCTCTGATGGAGGATGGGAAAGGTGGAGTCCTTGTAAGAGGTTTGGAGGAGGAAATTGTGACAAGTGCGAATGAGATTTTTACTCTTCTCGAAAGAGGGTCTTCTAAACGTCGTACTGCTGAAACTTTATTGAACAAGCAGTCAAGTCGGTCACATTCTCTCTTTTCCATCACCATACACATTAAAGAAGCAACCCCAGAAGGTGAAGAGCTGATCAAATGTGGAAAGTTGAATCTGGTTGATTTGGCCGGCTCAGAAAATATTTCTCGTTCGGGTGCTCGAGAGGGTCGTGCAAGGGAAGCCGGAGAAATCAACAAAAGTTTACTGACTTTAGGGCGAGTAATCAATGCCCTGGTGGAACATCTTGGGCATATCCCATACAGGGATAGTAAGCTAACACGATTACTTCGGGATTCACTTGGAGGAAGAACAAAGACCTGCATCATAGCTACAGTATCGCCTGCTGTGCACTGTCTTGAGGAAACCTTGAGTACGCTGGATTATGCACACAGAGCGAAAAATATAAGAAATAAACCTGAGGTGAATCAAAAAATGATGAAATCAACTCTTATCAAGGATCTTTATGGTGAAATTGAACGGCTTAAGGCAGAGGTGTATGCTGCGCGTGAGAAGAATGGTGTTTATATCCCGAAGGAGCGATACTATCAGGAAGAAAGTGAAAGGAAGTCCATGGCTGATCAGATTGAGAACATGGGGGTTGTAATAGAAAACCATCAGAAGCAATTTGAGGAGTTGCAGAATAAATATGATCTCCAGGTTCGACAGTGTTATGAATTGAGTAGCAAGCTTGATTCAACTGAGAAAACTTTGAACCATACCACTAAGTTACTTTCTACCACTGAGGAAGAATTGATGAAATGCCGGTATGCTTTGAAGGAGAGGGATTTTATCATTTCTGAGCAGAAGAAAGCTGAAAATGCTTTGGCTCAGCAAGCATGCAATTTGCAATCTGACTTGGAGAAAGCACTTCACGATAATGCATCCTTGTTTCTAAAAATTGGTAGAGAGGACAAGCTGAGTGCTGATAATAGGTTGGTAGTGAATAATTACCAGGAAGATCTTGCCAAACAAGTTGGTTCTCTTTGCCAGATTGTAGCAACATCGATGTCTCATCAGAATGAACACCTTCAGTGTGTAGAGAATCTTTGTCATTCTTTTTTGAGTGCACATGTTAAGGCAATTATGGACATGAAGAGTAAATTGACATCTTCAAGGACTTTGTATCTTTCTCATATTGAGGCAGTGCAAAATGTTGTGCGTTTGCACAAGGCGAGCTCTAATGCTGGTCTAGAGGAAATTTCATGTTTGGCTTCTTCTAATGTGCAGTCTGTTGAAGAATTTCTAACTTCAGAGGCTGGTGAAGCAGCTACAATATTTGAGGATCTTCAGAGCGGTCTCTCAACACAGCAGGGTGAAATGACAGCTTTTGCTAAGGAATTGAAACAGAGATTCAATTTGAGTATTAAGCAAATAAAAGACATTTCTGAGTACTCTCAAGGATTCCTACACAAGCTTTTGGAAGAATCAAAGAGGCTTGAAGATCATGTGGGGCAGACCAATGATATTAAATTAAATAGCATTGCTGAATTTCAGAAGGCTTATGAGGAGCAATCAAAATCTGATGCAGAGAAGCTTATTGCTGATATCTCTAGTTTAGTCTCCATTCACATGTGCCGTCAGAAAGAGATGGTGGATGCCAAGCTTGTTGGTTTCAGAGAAAGTGCTATTGCAGACAAGTATTTCATGGACGGTCATGTTTCCTCCATGGAGGGTATTACCACAGACGCAAAAAGAAAATGGCTTGAATTTTCAATGCAAGCAGAAAATGATGCTAAGGATGGTGTTGACTATTCTGCTGCTAAGCATTGCCGCATGGAGGTTATTCTACAAAAAAGTGTAAGTACTGCTGATACAGCTTTGGAGCACTGGAAAAAGACACAGGAGTCTGTGAATGATATGGGAAATAAACATGTTTCAGCTATGCTGTCACTTATCAGGAATGCTTCGGACAGTAATGAGCAACATGATGTCGAGATCAATTCAGTGAGGGCTGCAGTTGAGCAAGATGTGGCAAAGAATACTGATGATCTCCTTCAGCATGTTGATAGTGTGCGTGAGCAGGAGCAGGAATCCATATCTAAAATCTTAGAGACTATCAAGGCTCATACAAACACCCTTGAGACTTTCCGGGAGAGTCACTCGGGCCAAGCTGTTTCAATTGAGGAGAGGGCACGGGAAACCTTCCAGAACCATTATTTGGACTATGAACCTAGTGGATCTACGCCGGAAAAGTCTGAGCCGGAAGTTCCAAGCAAGGGCACCATTGAGTCACTTCGAGCCATGCCAATGGAAGCTCTTGTGGAGGAGTTCCGGGAAAACAATTCCTACGAGTCATTCGATGTGAAGGAGTTGAAACCGTCACTTATACCTCGCACTCCGCTTACACAACTGAACTAA

Protein Analysis

1010

Amino Acids

113.77

Weight (kDa)

5.48

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Microtub_bd PF16796 12 - 166 2.2e-24 Microtubule binding
Kinesin PF00225 18 - 359 1.2e-115 Kinesin motor domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010872)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36200 AT2G36200
fragaria_vesca FvH4_6g19220
malus_domestica MD12G1028000.v1.1 MD14G1028400.v1.1
prunus_persica Prupe.7G103600_v2.0.a1 Prupe.7G103600_v2.0.a1
pyrus_communis pycom12g02680 pycom14g02650
rosa_chinensis RchiOBHm_Chr3g0474331
rosa_laevigata RLG00000023932
rosa_multiflora Rmu_sc0000098.1_g000007 Rmu_sc0004507.1_g000011
rosa_roxburghii Rroxscaffold_6G00407340
rosa_rugosa Rorug03G0140100
rosa_samantha Rh3AG190900 Rh3BG220100 Rh3CG215300 Rh3DG215800
rosa_wichuraiana Rw3G017410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2515
Acc36I ACCTGC 2 cut(s) 88, 998
Acc65I GGTACC 1 cut(s) 302
AccB1I GGYRCC 2 cut(s) 302, 2891
AccB7I CCANNNNNTGG 1 cut(s) 2830
AccBSI CCGCTC 1 cut(s) 1965
AccII CGCG 1 cut(s) 1177
AciI CCGC 6 cut(s) 10, 82, 95, 1965, 2443, 3011
AclWI GGATC 3 cut(s) 1137, 1962, 2857
AcoI YGGCCR 3 cut(s) 7, 132, 798
AcsI RAATTY 5 cut(s) 332, 1869, 1910, 2159, 2378
AcuI CTGAAG 4 cut(s) 1685, 1905, 1943, 2651
AfaI GTAC 8 cut(s) 119, 277, 304, 443, 667, 1045, 2063, 2476
AfiI CCNNNNNNNGG 5 cut(s) 380, 934, 2449, 2830, 2888
AflII CTTAAG 1 cut(s) 1154
AflIII ACRYGT 3 cut(s) 1741, 2241, 2542
AgeI ACCGGT 1 cut(s) 305
AjnI CCWGG 4 cut(s) 39, 903, 1326, 1623
AjuI GAANNNNNNNTTGG 2 cut(s) 2813, 2845
Alw21I GWGCWC 6 cut(s) 252, 832, 1023, 1741, 1850, 2499
Alw26I GTCTC 6 cut(s) 144, 1686, 1973, 2236, 2717, 2747
Alw44I GTGCAC 3 cut(s) 248, 1019, 1737
AlwI GGATC 3 cut(s) 1137, 1962, 2857
AlwNI CAGNNNCTG 1 cut(s) 1928
Ama87I CYCGRG 3 cut(s) 374, 831, 2773
AoxI GGCC 7 cut(s) 7, 59, 132, 353, 427, 798, 2776
ApaLI GTGCAC 3 cut(s) 248, 1019, 1737
ApeKI GCWGC 6 cut(s) 547, 550, 1172, 1937, 2429, 2624
ApoI RAATTY 5 cut(s) 332, 1869, 1910, 2159, 2378
AsiGI ACCGGT 1 cut(s) 305
Asp700I GAANNNNTTC 2 cut(s) 1698, 1911
Asp718I GGTACC 1 cut(s) 302
AspLEI GCGC 1 cut(s) 1177
AspS9I GGNCC 5 cut(s) 43, 197, 353, 427, 2776
AsuC2I CCSGG 2 cut(s) 2762, 2945
AsuHPI GGTGA 7 cut(s) 341, 715, 767, 1105, 1151, 1943, 1997
AvaI CYCGRG 3 cut(s) 374, 831, 2773
AvaII GGWCC 2 cut(s) 43, 197
AxyI CCTNAGG 1 cut(s) 1089
BaeGI GKGCMC 5 cut(s) 252, 1023, 1741, 2806, 2894
BanI GGYRCC 2 cut(s) 302, 2891
BanII GRGCYC 1 cut(s) 1850
BarI GAAGNNNNNNTAC 2 cut(s) 1196, 1228
BauI CACGAG 1 cut(s) 14
BbsI GAAGAC 1 cut(s) 645
Bbv12I GWGCWC 6 cut(s) 252, 832, 1023, 1741, 1850, 2499
BbvI GCAGC 6 cut(s) 537, 559, 1159, 1949, 2416, 2611
BccI CCATC 6 cut(s) 553, 560, 728, 1293, 2254, 2405
BceAI ACGGC 4 cut(s) 46, 119, 1166, 2232
BcgI CGANNNNNNTGC 2 cut(s) 680, 714
BciT130I CCWGG 4 cut(s) 41, 905, 1328, 1625
BclI TGATCA 3 cut(s) 226, 765, 1249
BcnI CCSGG 2 cut(s) 2762, 2945
BcoDI GTCTC 6 cut(s) 144, 1686, 1973, 2236, 2717, 2747
BfaI CTAG 3 cut(s) 1862, 2223, 2844
BfmI CTRYAG 2 cut(s) 1002, 2625
BfrI CTTAAG 1 cut(s) 1154
BfuAI ACCTGC 2 cut(s) 88, 998
BglII AGATCT 2 cut(s) 217, 1630
BisI GCNGC 8 cut(s) 10, 548, 551, 1173, 1938, 2430, 2443, 2625
BlpI GCTNAGC 3 cut(s) 207, 1500, 2433
BlsI GCNGC 8 cut(s) 11, 549, 552, 1174, 1939, 2431, 2444, 2626
BmcAI AGTACT 2 cut(s) 2063, 2476
Bme1390I CCNGG 6 cut(s) 41, 905, 1328, 1625, 2762, 2945
Bme18I GGWCC 2 cut(s) 43, 197
BmeT110I CYCGRG 3 cut(s) 374, 831, 2773
BmgT120I GGNCC 5 cut(s) 43, 197, 353, 427, 2776
BmiI GGNNCC 2 cut(s) 304, 2893
BmrFI CCNGG 6 cut(s) 41, 905, 1328, 1625, 2762, 2945
BmrI ACTGGG 1 cut(s) 360
BmsI GCATC 5 cut(s) 1002, 1562, 2185, 2257, 2392
BmuI ACTGGG 1 cut(s) 360
BpiI GAAGAC 1 cut(s) 645
BplI GAGNNNNNCTC 2 cut(s) 2756, 2788
BpmI CTGGAG 3 cut(s) 402, 486, 1310
Bpu10I CCTNAGC 2 cut(s) 2004, 2406
Bpu1102I GCTNAGC 3 cut(s) 207, 1500, 2433
BpuEI CTTGAG 5 cut(s) 443, 1049, 1060, 2052, 2771
BpuMI CCSGG 2 cut(s) 2762, 2945
Bsa29I ATCGAT 2 cut(s) 165, 1676
BsaI GGTCTC 1 cut(s) 1973
BsaJI CCNNGG 5 cut(s) 375, 392, 903, 1242, 2337
BsaWI WCCGGW 1 cut(s) 305
BsaXI ACNNNNNCTCC 2 cut(s) 570, 600
Bsc4I CCNNNNNNNGG 5 cut(s) 380, 934, 2449, 2830, 2888
Bse118I RCCGGY 3 cut(s) 305, 800, 1434
Bse1I ACTGG 3 cut(s) 304, 355, 2504
Bse21I CCTNAGG 1 cut(s) 1089
Bse3DI GCAATG 4 cut(s) 94, 147, 2151, 2437
BseBI CCWGG 4 cut(s) 41, 905, 1328, 1625
BseCI ATCGAT 2 cut(s) 165, 1676
BseDI CCNNGG 5 cut(s) 375, 392, 903, 1242, 2337
BseGI GGATG 5 cut(s) 79, 571, 1553, 2272, 2416
BseLI CCNNNNNNNGG 5 cut(s) 380, 934, 2449, 2830, 2888
BseMI GCAATG 4 cut(s) 94, 147, 2151, 2437
BseNI ACTGG 3 cut(s) 304, 355, 2504
BseRI GAGGAG 5 cut(s) 614, 1316, 2192, 2810, 2951
BseSI GKGCMC 5 cut(s) 252, 1023, 1741, 2806, 2894
BseX3I CGGCCG 1 cut(s) 7
BseXI GCAGC 6 cut(s) 537, 559, 1159, 1949, 2416, 2611
BsgI GTGCAG 1 cut(s) 1916
Bsh1236I CGCG 1 cut(s) 1177
Bsh1285I CGRYCG 1 cut(s) 10
BshFI GGCC 7 cut(s) 9, 61, 134, 355, 429, 800, 2778
BshNI GGYRCC 2 cut(s) 302, 2891
BshTI ACCGGT 1 cut(s) 305
BshVI ATCGAT 2 cut(s) 165, 1676
BsiEI CGRYCG 1 cut(s) 10
BsiHKAI GWGCWC 6 cut(s) 252, 832, 1023, 1741, 1850, 2499
BsiHKCI CYCGRG 3 cut(s) 374, 831, 2773
BslI CCNNNNNNNGG 5 cut(s) 380, 934, 2449, 2830, 2888
BsmAI GTCTC 6 cut(s) 144, 1686, 1973, 2236, 2717, 2747
BsmI GAATGC 2 cut(s) 439, 2578
BsnI GGCC 7 cut(s) 9, 61, 134, 355, 429, 800, 2778
Bso31I GGTCTC 1 cut(s) 1973
BsoBI CYCGRG 3 cut(s) 374, 831, 2773
Bsp1286I GDGCHC 8 cut(s) 252, 832, 1023, 1741, 1850, 2499, 2806, 2894
Bsp1720I GCTNAGC 3 cut(s) 207, 1500, 2433
Bsp19I CCATGG 2 cut(s) 1242, 2337
BspACI CCGC 6 cut(s) 10, 82, 95, 1965, 2443, 3011
BspANI GGCC 7 cut(s) 9, 61, 134, 355, 429, 800, 2778
BspDI ATCGAT 2 cut(s) 165, 1676
BspFNI CGCG 1 cut(s) 1177
BspLI GGNNCC 2 cut(s) 304, 2893
BspMAI CTGCAG 1 cut(s) 2629
BspMI ACCTGC 2 cut(s) 88, 998
BspPI GGATC 3 cut(s) 1137, 1962, 2857
BspQI GCTCTTC 1 cut(s) 753
BspT107I GGYRCC 2 cut(s) 302, 2891
BspTI CTTAAG 1 cut(s) 1154
BspTNI GGTCTC 1 cut(s) 1973
BsrBI CCGCTC 1 cut(s) 1965
BsrDI GCAATG 4 cut(s) 94, 147, 2151, 2437
BsrFI RCCGGY 3 cut(s) 305, 800, 1434
BsrI ACTGG 3 cut(s) 304, 355, 2504
BssAI RCCGGY 3 cut(s) 305, 800, 1434
BssECI CCNNGG 5 cut(s) 375, 392, 903, 1242, 2337
BssSI CACGAG 1 cut(s) 14
BssT1I CCWWGG 3 cut(s) 392, 1242, 2337
Bst2BI CACGAG 1 cut(s) 14
Bst2UI CCWGG 4 cut(s) 41, 905, 1328, 1625
Bst4CI ACNGT 8 cut(s) 275, 446, 1006, 1025, 1338, 2324, 2585, 2991
Bst6I CTCTTC 4 cut(s) 474, 642, 753, 1760
BstAFI CTTAAG 1 cut(s) 1154
BstC8I GCNNGC 8 cut(s) 59, 802, 1014, 1358, 1506, 1510, 1846, 2391
BstDSI CCRYGG 2 cut(s) 1242, 2337
BstF5I GGATG 5 cut(s) 79, 571, 1553, 2272, 2416
BstFNI CGCG 1 cut(s) 1177
BstHHI GCGC 1 cut(s) 1177
BstMAI GTCTC 6 cut(s) 144, 1686, 1973, 2236, 2717, 2747
BstMCI CGRYCG 1 cut(s) 10
BstMWI GCNNNNNNNGC 8 cut(s) 544, 851, 1018, 1064, 1490, 1505, 1934, 2486
BstNI CCWGG 4 cut(s) 41, 905, 1328, 1625
BstNSI RCATGY 5 cut(s) 1512, 1745, 2245, 2546, 2675
BstSCI CCNGG 6 cut(s) 39, 903, 1326, 1623, 2760, 2943
BstSFI CTRYAG 2 cut(s) 1002, 2625
BstSLI GKGCMC 5 cut(s) 252, 1023, 1741, 2806, 2894
BstUI CGCG 1 cut(s) 1177
BstV1I GCAGC 6 cut(s) 537, 559, 1159, 1949, 2416, 2611
BstV2I GAAGAC 1 cut(s) 645
BstX2I RGATCY 5 cut(s) 217, 1129, 1630, 1954, 2849
BstXI CCANNNNNNTGG 1 cut(s) 2920
BstYI RGATCY 5 cut(s) 217, 1129, 1630, 1954, 2849
BstZI CGGCCG 1 cut(s) 7
Bsu15I ATCGAT 2 cut(s) 165, 1676
Bsu36I CCTNAGG 1 cut(s) 1089
BsuRI GGCC 7 cut(s) 9, 61, 134, 355, 429, 800, 2778
BsuTUI ATCGAT 2 cut(s) 165, 1676
BtgI CCRYGG 2 cut(s) 1242, 2337
BtsCI GGATG 5 cut(s) 79, 571, 1553, 2272, 2416
BtsI GCAGTG 1 cut(s) 1821
BtsIMutI CAGTG 8 cut(s) 451, 1021, 1343, 1410, 1709, 1821, 2497, 2622
BveI ACCTGC 2 cut(s) 88, 998
Cac8I GCNNGC 8 cut(s) 59, 802, 1014, 1358, 1506, 1510, 1846, 2391
CaiI CAGNNNCTG 1 cut(s) 1928
CfoI GCGC 1 cut(s) 1177
Cfr10I RCCGGY 3 cut(s) 305, 800, 1434
Cfr13I GGNCC 5 cut(s) 43, 197, 353, 427, 2776
ClaI ATCGAT 2 cut(s) 165, 1676
CseI GACGC 1 cut(s) 2366
Csp6I GTAC 8 cut(s) 118, 276, 303, 442, 666, 1044, 2062, 2475
CspAI ACCGGT 1 cut(s) 305
CviQI GTAC 8 cut(s) 118, 276, 303, 442, 666, 1044, 2062, 2475
DrdI GACNNNNNNGTC 1 cut(s) 2515
DseDI GACNNNNNNGTC 1 cut(s) 2515
EaeI YGGCCR 3 cut(s) 7, 132, 798
EagI CGGCCG 1 cut(s) 7
Eam1104I CTCTTC 4 cut(s) 474, 642, 753, 1760
EarI CTCTTC 4 cut(s) 474, 642, 753, 1760
Ecl136II GAGCTC 1 cut(s) 1848
EclXI CGGCCG 1 cut(s) 7
Eco130I CCWWGG 3 cut(s) 392, 1242, 2337
Eco24I GRGCYC 1 cut(s) 1850
Eco31I GGTCTC 1 cut(s) 1973
Eco32I GATATC 1 cut(s) 2218
Eco47I GGWCC 2 cut(s) 43, 197
Eco52I CGGCCG 1 cut(s) 7
Eco53kI GAGCTC 1 cut(s) 1848
Eco57I CTGAAG 4 cut(s) 1685, 1905, 1943, 2651
Eco81I CCTNAGG 1 cut(s) 1089
Eco88I CYCGRG 3 cut(s) 374, 831, 2773
EcoICRI GAGCTC 1 cut(s) 1848
EcoO109I RGGNCCY 1 cut(s) 43
EcoRI GAATTC 1 cut(s) 332
EcoRII CCWGG 4 cut(s) 39, 903, 1326, 1623
EcoRV GATATC 1 cut(s) 2218
EcoT14I CCWWGG 3 cut(s) 392, 1242, 2337
EcoT22I ATGCAT 1 cut(s) 1555
EcoT38I GRGCYC 1 cut(s) 1850
ErhI CCWWGG 3 cut(s) 392, 1242, 2337
FalI AAGNNNNNCTT 2 cut(s) 637, 669
FauI CCCGC 1 cut(s) 102
FbaI TGATCA 3 cut(s) 226, 765, 1249
Fnu4HI GCNGC 8 cut(s) 10, 548, 551, 1173, 1938, 2430, 2443, 2625
FokI GGATG 5 cut(s) 86, 578, 1540, 2279, 2423
FriOI GRGCYC 1 cut(s) 1850
Fsp4HI GCNGC 8 cut(s) 10, 548, 551, 1173, 1938, 2430, 2443, 2625
FspBI CTAG 3 cut(s) 1862, 2223, 2844
GlaI GCGC 1 cut(s) 1176
GluI GCNGC 8 cut(s) 10, 548, 551, 1173, 1938, 2430, 2443, 2625
GsuI CTGGAG 3 cut(s) 402, 486, 1310
HaeIII GGCC 7 cut(s) 9, 61, 134, 355, 429, 800, 2778
HgaI GACGC 1 cut(s) 2366
HhaI GCGC 1 cut(s) 1177
Hin6I GCGC 1 cut(s) 1175
HinP1I GCGC 1 cut(s) 1175
HincII GTYRAC 2 cut(s) 34, 2419
HindII GTYRAC 2 cut(s) 34, 2419
HindIII AAGCTT 4 cut(s) 1358, 2084, 2204, 2273
HpaI GTTAAC 1 cut(s) 34
HphI GGTGA 7 cut(s) 341, 715, 767, 1105, 1151, 1943, 1997
Hpy166II GTNNAC 6 cut(s) 34, 250, 875, 1021, 1739, 2419
Hpy8I GTNNAC 6 cut(s) 34, 250, 875, 1021, 1739, 2419
Hpy99I CGWCG 1 cut(s) 666
HpyCH4III ACNGT 8 cut(s) 275, 446, 1006, 1025, 1338, 2324, 2585, 2991
HpyCH4IV ACGT 2 cut(s) 36, 661
HpyF10VI GCNNNNNNNGC 8 cut(s) 544, 851, 1018, 1064, 1490, 1505, 1934, 2486
HpySE526I ACGT 2 cut(s) 36, 661
HspAI GCGC 1 cut(s) 1175
KpnI GGTACC 1 cut(s) 306
KroI GCCGGC 1 cut(s) 800
KroNI GCCGGC 1 cut(s) 802
Ksp22I TGATCA 3 cut(s) 226, 765, 1249
KspAI GTTAAC 1 cut(s) 34
LguI GCTCTTC 1 cut(s) 753
LmnI GCTCC 6 cut(s) 84, 505, 1207, 2179, 2494, 2695
Lsp1109I GCAGC 6 cut(s) 537, 559, 1159, 1949, 2416, 2611
LweI GCATC 5 cut(s) 1002, 1562, 2185, 2257, 2392
MaeI CTAG 3 cut(s) 1862, 2223, 2844
MaeII ACGT 2 cut(s) 36, 661
MaeIII GTNAC 8 cut(s) 454, 610, 702, 1398, 2559, 2768, 2901, 2991
MbiI CCGCTC 1 cut(s) 1965
MfeI CAATTG 1 cut(s) 2790
MflI RGATCY 5 cut(s) 217, 1129, 1630, 1954, 2849
MhlI GDGCHC 8 cut(s) 252, 832, 1023, 1741, 1850, 2499, 2806, 2894
MlyI GAGTC 5 cut(s) 588, 2525, 2776, 2909, 2972
Mph1103I ATGCAT 1 cut(s) 1555
MroNI GCCGGC 1 cut(s) 800
MroXI GAANNNNTTC 2 cut(s) 1698, 1911
MseI TTAA 8 cut(s) 33, 513, 735, 1155, 1746, 2037, 2139, 2144
MslI CAYNNNNRTG 4 cut(s) 1548, 1689, 2240, 2918
MspA1I CMGCKG 1 cut(s) 550
MspCI CTTAAG 1 cut(s) 1154
MspR9I CCNGG 6 cut(s) 41, 905, 1328, 1625, 2762, 2945
MunI CAATTG 1 cut(s) 2790
Mva1269I GAATGC 2 cut(s) 439, 2578
MvaI CCWGG 4 cut(s) 41, 905, 1328, 1625
MvnI CGCG 1 cut(s) 1177
MwoI GCNNNNNNNGC 8 cut(s) 544, 851, 1018, 1064, 1490, 1505, 1934, 2486
NaeI GCCGGC 1 cut(s) 802
NciI CCSGG 2 cut(s) 2762, 2945
NcoI CCATGG 2 cut(s) 1242, 2337
NgoMIV GCCGGC 1 cut(s) 800
NlaIV GGNNCC 2 cut(s) 304, 2893
NmuCI GTSAC 7 cut(s) 454, 610, 702, 2559, 2768, 2901, 2991
NsiI ATGCAT 1 cut(s) 1555
NspI RCATGY 5 cut(s) 1512, 1745, 2245, 2546, 2675
PaeI GCATGC 1 cut(s) 1512
PaeR7I CTCGAG 1 cut(s) 831
PciI ACATGT 3 cut(s) 1741, 2241, 2542
PciSI GCTCTTC 1 cut(s) 753
PcsI WCGNNNNNNNCGW 2 cut(s) 838, 2967
PctI GAATGC 2 cut(s) 439, 2578
PdiI GCCGGC 1 cut(s) 802
PdmI GAANNNNTTC 2 cut(s) 1698, 1911
PflMI CCANNNNNTGG 1 cut(s) 2830
PfoI TCCNGGA 2 cut(s) 2760, 2943
PinAI ACCGGT 1 cut(s) 305
PkrI GCNGC 8 cut(s) 11, 549, 552, 1174, 1939, 2431, 2444, 2626
PleI GAGTC 5 cut(s) 587, 2524, 2775, 2908, 2971
PpsI GAGTC 5 cut(s) 587, 2524, 2775, 2908, 2971
PpuMI RGGWCCY 1 cut(s) 43
PscI ACATGT 3 cut(s) 1741, 2241, 2542
Psp124BI GAGCTC 1 cut(s) 1850
Psp5II RGGWCCY 1 cut(s) 43
Psp6I CCWGG 4 cut(s) 39, 903, 1326, 1623
PspGI CCWGG 4 cut(s) 39, 903, 1326, 1623
PspN4I GGNNCC 2 cut(s) 304, 2893
PspPI GGNCC 5 cut(s) 43, 197, 353, 427, 2776
PspPPI RGGWCCY 1 cut(s) 43
PstI CTGCAG 1 cut(s) 2629
PstNI CAGNNNCTG 1 cut(s) 1928
PsuI RGATCY 5 cut(s) 217, 1129, 1630, 1954, 2849
PvuII CAGCTG 1 cut(s) 550
RsaI GTAC 8 cut(s) 119, 277, 304, 443, 667, 1045, 2063, 2476
RsaNI GTAC 8 cut(s) 118, 276, 303, 442, 666, 1044, 2062, 2475
RseI CAYNNNNRTG 4 cut(s) 1548, 1689, 2240, 2918
SacI GAGCTC 1 cut(s) 1850
SapI GCTCTTC 1 cut(s) 753
SaqAI TTAA 8 cut(s) 33, 513, 735, 1155, 1746, 2037, 2139, 2144
SatI GCNGC 8 cut(s) 10, 548, 551, 1173, 1938, 2430, 2443, 2625
Sau96I GGNCC 5 cut(s) 43, 197, 353, 427, 2776
ScaI AGTACT 2 cut(s) 2063, 2476
SchI GAGTC 5 cut(s) 588, 2525, 2776, 2909, 2972
ScrFI CCNGG 6 cut(s) 41, 905, 1328, 1625, 2762, 2945
SduI GDGCHC 8 cut(s) 252, 832, 1023, 1741, 1850, 2499, 2806, 2894
SfaNI GCATC 5 cut(s) 1002, 1562, 2185, 2257, 2392
SfcI CTRYAG 2 cut(s) 1002, 2625
Sfr274I CTCGAG 1 cut(s) 831
SinI GGWCC 2 cut(s) 43, 197
SlaI CTCGAG 1 cut(s) 831
SmiMI CAYNNNNRTG 4 cut(s) 1548, 1689, 2240, 2918
SmlI CTYRAG 7 cut(s) 422, 831, 1028, 1039, 1154, 2067, 2750
SmoI CTYRAG 7 cut(s) 422, 831, 1028, 1039, 1154, 2067, 2750
SphI GCATGC 1 cut(s) 1512
SsiI CCGC 6 cut(s) 10, 82, 95, 1965, 2443, 3011
SspI AATATT 2 cut(s) 814, 1947
SspMI CTAG 3 cut(s) 1862, 2223, 2844
SstI GAGCTC 1 cut(s) 1850
StyD4I CCNGG 6 cut(s) 39, 903, 1326, 1623, 2760, 2943
StyI CCWWGG 3 cut(s) 392, 1242, 2337
TaaI ACNGT 8 cut(s) 275, 446, 1006, 1025, 1338, 2324, 2585, 2991
TaiI ACGT 2 cut(s) 39, 664
TaqII GACCGA 1 cut(s) 690
TatI WGTACW 3 cut(s) 441, 2061, 2474
TauI GCSGC 2 cut(s) 12, 2445
Tru1I TTAA 8 cut(s) 33, 513, 735, 1155, 1746, 2037, 2139, 2144
Tru9I TTAA 8 cut(s) 33, 513, 735, 1155, 1746, 2037, 2139, 2144
TscAI CASTG 8 cut(s) 451, 1028, 1343, 1417, 1709, 1821, 2504, 2622
TseFI GTSAC 7 cut(s) 454, 610, 702, 2559, 2768, 2901, 2991
TseI GCWGC 6 cut(s) 547, 550, 1172, 1937, 2429, 2624
Tsp45I GTSAC 7 cut(s) 454, 610, 702, 2559, 2768, 2901, 2991
TspDTI ATGAA 8 cut(s) 1124, 1359, 1442, 1707, 1778, 1863, 2305, 2853
TspGWI ACGGA 1 cut(s) 41
TspRI CASTG 8 cut(s) 451, 1028, 1343, 1417, 1709, 1821, 2504, 2622
Van91I CCANNNNNTGG 1 cut(s) 2830
Vha464I CTTAAG 1 cut(s) 1154
VneI GTGCAC 3 cut(s) 248, 1019, 1737
VpaK11BI GGWCC 2 cut(s) 43, 197
XapI RAATTY 5 cut(s) 332, 1869, 1910, 2159, 2378
XbaI TCTAGA 1 cut(s) 1861
XceI RCATGY 5 cut(s) 1512, 1745, 2245, 2546, 2675
XhoI CTCGAG 1 cut(s) 831
XmnI GAANNNNTTC 2 cut(s) 1698, 1911
XspI CTAG 3 cut(s) 1862, 2223, 2844
ZrmI AGTACT 2 cut(s) 2063, 2476
Zsp2I ATGCAT 1 cut(s) 1555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.