MD12G1035000.v1.1

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016885
Physical Location & Seq
Forward (+)
3828970 .. 3831219
2250 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1035000.v1.1.491

Sequence Viewer

Length: 597 bp
ATGGGTTCTAAACAATGTTGTTGCTGCCGCTGCCTCCTTTTCTTGGGTCTTTTCTTCATCGGCATCGCTGTTCTGTTCGTGTTTCGGGATAAACTAGGCCCTTCGTTAGAAGCGAGCTACAAGGTGACTGATGCCGTGTTGACGCAGTTTGGTACGGGGTCTGGAAAAACCCTCGAATACAACCTAGTTGCCAACATAACCGTCGAAAATCGTGAAAAAAATTTTGACTACAAGTATGACAAATTTGAAGCTGTTGCTACTTATGATGACAAGGATTTAAGTGCAGTTAGTTTGAGCACATTCGATGTAGCCAGCACGAAGACAAGTGCAGTGACTCCACTAGTGTTCAAGGGGCAGAAGTCTCTTAAGGGCGATGTGGCTTCAAAAATTAAGGGTGGTACGAGTTTTGATATCGTTTTGAAGATCAAAATTAAGGTTCTGTCCAGGTTTACTAAGGTAGATATTTTTAACGAGTATAAGGTGGAATGCAAGTTGAAGGTTCCTTTGGTAAATTCCAAGGGAAAATCTTCTGACAAATTTGAGAGTACCGATTGCGAAAAGGTTTCTGGAAGCACTAGTAAAGGAATAAAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

199

Amino Acids

21.75

Weight (kDa)

9.13

Isoelectric Point (pI)

9.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016885)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g18520
malus_domestica MD12G1035000.v1.1
prunus_persica Prupe.7G112100_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0473081
rosa_laevigata RLG00000024030
rosa_multiflora Rmu_sc0006336.1_g000002 Rmu_sc0006336.1_g000003
rosa_roxburghii Rroxscaffold_6G00408330
rosa_rugosa Rorug03G0131400
rosa_samantha Rh3AG182800 Rh3BG209300 Rh3CG206300 Rh3DG206300
rosa_wichuraiana Rw3G016020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 28
AcsI RAATTY 4 cut(s) 220, 242, 511, 536
AfaI GTAC 3 cut(s) 154, 400, 547
AfiI CCNNNNNNNGG 1 cut(s) 43
AflII CTTAAG 1 cut(s) 365
AgsI TTSAA 5 cut(s) 248, 349, 384, 421, 496
AhlI ACTAGT 2 cut(s) 340, 575
AjnI CCWGG 1 cut(s) 443
AjuI GAANNNNNNNTTGG 2 cut(s) 488, 520
AluBI AGCT 2 cut(s) 117, 251
AluI AGCT 2 cut(s) 117, 251
Alw21I GWGCWC 1 cut(s) 299
Alw26I GTCTC 1 cut(s) 366
AoxI GGCC 1 cut(s) 97
ApeKI GCWGC 2 cut(s) 24, 30
ApoI RAATTY 4 cut(s) 220, 242, 511, 536
Asp700I GAANNNNTTC 1 cut(s) 526
AspS9I GGNCC 1 cut(s) 98
AsuHPI GGTGA 1 cut(s) 136
BbsI GAAGAC 1 cut(s) 326
Bbv12I GWGCWC 1 cut(s) 299
BbvI GCAGC 2 cut(s) 11, 17
BceAI ACGGC 1 cut(s) 119
BciT130I CCWGG 1 cut(s) 445
BcoDI GTCTC 1 cut(s) 366
BcuI ACTAGT 2 cut(s) 340, 575
BfaI CTAG 4 cut(s) 95, 185, 341, 576
BfrI CTTAAG 1 cut(s) 365
BisI GCNGC 3 cut(s) 25, 28, 31
BlsI GCNGC 3 cut(s) 26, 29, 32
Bme1390I CCNGG 1 cut(s) 445
BmgT120I GGNCC 1 cut(s) 98
BmiI GGNNCC 1 cut(s) 501
BmrFI CCNGG 1 cut(s) 445
BmsI GCATC 2 cut(s) 72, 121
BpiI GAAGAC 1 cut(s) 326
BsaJI CCNNGG 1 cut(s) 516
Bsc4I CCNNNNNNNGG 1 cut(s) 43
BseBI CCWGG 1 cut(s) 445
BseDI CCNNGG 1 cut(s) 516
BseLI CCNNNNNNNGG 1 cut(s) 43
BseXI GCAGC 2 cut(s) 11, 17
BsgI GTGCAG 2 cut(s) 303, 348
BshFI GGCC 1 cut(s) 99
BsiHKAI GWGCWC 1 cut(s) 299
BslI CCNNNNNNNGG 1 cut(s) 43
BsmAI GTCTC 1 cut(s) 366
BsmI GAATGC 1 cut(s) 491
BsnI GGCC 1 cut(s) 99
Bsp1286I GDGCHC 1 cut(s) 299
Bsp143I GATC 1 cut(s) 423
BspACI CCGC 1 cut(s) 28
BspANI GGCC 1 cut(s) 99
BspLI GGNNCC 1 cut(s) 501
BspTI CTTAAG 1 cut(s) 365
BssECI CCNNGG 1 cut(s) 516
BssMI GATC 1 cut(s) 423
BssT1I CCWWGG 1 cut(s) 516
Bst2UI CCWGG 1 cut(s) 445
Bst4CI ACNGT 1 cut(s) 202
BstAFI CTTAAG 1 cut(s) 365
BstC8I GCNNGC 2 cut(s) 115, 313
BstDEI CTNAG 1 cut(s) 453
BstKTI GATC 1 cut(s) 426
BstMAI GTCTC 1 cut(s) 366
BstMBI GATC 1 cut(s) 423
BstMWI GCNNNNNNNGC 1 cut(s) 30
BstNI CCWGG 1 cut(s) 445
BstSCI CCNGG 1 cut(s) 443
BstV1I GCAGC 2 cut(s) 11, 17
BstV2I GAAGAC 1 cut(s) 326
BsuRI GGCC 1 cut(s) 99
BtgZI GCGATG 2 cut(s) 49, 387
BtsI GCAGTG 1 cut(s) 336
BtsIMutI CAGTG 1 cut(s) 336
Cac8I GCNNGC 2 cut(s) 115, 313
Cfr13I GGNCC 1 cut(s) 98
CseI GACGC 1 cut(s) 151
Csp6I GTAC 3 cut(s) 153, 399, 546
CviJI RGCY 5 cut(s) 99, 117, 251, 311, 380
CviKI_1 RGCY 5 cut(s) 99, 117, 251, 311, 380
CviQI GTAC 3 cut(s) 153, 399, 546
DdeI CTNAG 1 cut(s) 453
DpnI GATC 1 cut(s) 425
DpnII GATC 1 cut(s) 423
Eco130I CCWWGG 1 cut(s) 516
Eco32I GATATC 1 cut(s) 412
EcoO109I RGGNCCY 1 cut(s) 98
EcoRII CCWGG 1 cut(s) 443
EcoRV GATATC 1 cut(s) 412
EcoT14I CCWWGG 1 cut(s) 516
ErhI CCWWGG 1 cut(s) 516
FaiI YATR 4 cut(s) 197, 237, 264, 477
Fnu4HI GCNGC 3 cut(s) 25, 28, 31
Fsp4HI GCNGC 3 cut(s) 25, 28, 31
FspBI CTAG 4 cut(s) 95, 185, 341, 576
GluI GCNGC 3 cut(s) 25, 28, 31
HaeIII GGCC 1 cut(s) 99
HgaI GACGC 1 cut(s) 151
HincII GTYRAC 1 cut(s) 141
HindII GTYRAC 1 cut(s) 141
HinfI GANTC 1 cut(s) 334
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 2 cut(s) 141, 450
Hpy188I TCNGA 1 cut(s) 532
Hpy188III TCNNGA 4 cut(s) 86, 162, 212, 567
Hpy8I GTNNAC 2 cut(s) 141, 450
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 2 cut(s) 111, 490
HpyCH4III ACNGT 1 cut(s) 202
HpyCH4V TGCA 3 cut(s) 284, 329, 489
HpyF10VI GCNNNNNNNGC 1 cut(s) 30
HpyF3I CTNAG 1 cut(s) 453
Kzo9I GATC 1 cut(s) 423
LpnPI CCDG 5 cut(s) 147, 325, 430, 457, 552
Lsp1109I GCAGC 2 cut(s) 11, 17
LweI GCATC 2 cut(s) 72, 121
MaeI CTAG 4 cut(s) 95, 185, 341, 576
MaeIII GTNAC 2 cut(s) 124, 331
MalI GATC 1 cut(s) 425
MboI GATC 1 cut(s) 423
MboII GAAGA 4 cut(s) 46, 331, 433, 519
MhlI GDGCHC 1 cut(s) 299
MluCI AATT 6 cut(s) 220, 242, 387, 429, 511, 536
MlyI GAGTC 1 cut(s) 328
MnlI CCTC 2 cut(s) 44, 182
MroXI GAANNNNTTC 1 cut(s) 526
MseI TTAA 5 cut(s) 278, 366, 390, 432, 468
MspA1I CMGCKG 1 cut(s) 30
MspCI CTTAAG 1 cut(s) 365
MspR9I CCNGG 1 cut(s) 445
Mva1269I GAATGC 1 cut(s) 491
MvaI CCWGG 1 cut(s) 445
MwoI GCNNNNNNNGC 1 cut(s) 30
NdeII GATC 1 cut(s) 423
NlaIV GGNNCC 1 cut(s) 501
NmuCI GTSAC 2 cut(s) 124, 331
PcsI WCGNNNNNNNCGW 1 cut(s) 110
PctI GAATGC 1 cut(s) 491
PdmI GAANNNNTTC 1 cut(s) 526
PkrI GCNGC 3 cut(s) 26, 29, 32
PleI GAGTC 1 cut(s) 328
PpsI GAGTC 1 cut(s) 328
Psp6I CCWGG 1 cut(s) 443
PspGI CCWGG 1 cut(s) 443
PspN4I GGNNCC 1 cut(s) 501
PspPI GGNCC 1 cut(s) 98
RsaI GTAC 3 cut(s) 154, 400, 547
RsaNI GTAC 3 cut(s) 153, 399, 546
SaqAI TTAA 5 cut(s) 278, 366, 390, 432, 468
SatI GCNGC 3 cut(s) 25, 28, 31
Sau3AI GATC 1 cut(s) 423
Sau96I GGNCC 1 cut(s) 98
SchI GAGTC 1 cut(s) 328
ScrFI CCNGG 1 cut(s) 445
SduI GDGCHC 1 cut(s) 299
SfaNI GCATC 2 cut(s) 72, 121
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
SpeI ACTAGT 2 cut(s) 340, 575
Sse9I AATT 6 cut(s) 220, 242, 387, 429, 511, 536
SsiI CCGC 1 cut(s) 28
SspMI CTAG 4 cut(s) 95, 185, 341, 576
StyD4I CCNGG 1 cut(s) 443
StyI CCWWGG 1 cut(s) 516
TaaI ACNGT 1 cut(s) 202
TaqI TCGA 3 cut(s) 174, 204, 303
TasI AATT 6 cut(s) 220, 242, 387, 429, 511, 536
TauI GCSGC 1 cut(s) 30
Tru1I TTAA 5 cut(s) 278, 366, 390, 432, 468
Tru9I TTAA 5 cut(s) 278, 366, 390, 432, 468
TscAI CASTG 1 cut(s) 336
TseFI GTSAC 2 cut(s) 124, 331
TseI GCWGC 2 cut(s) 24, 30
Tsp45I GTSAC 2 cut(s) 124, 331
TspDTI ATGAA 1 cut(s) 46
TspRI CASTG 1 cut(s) 336
Vha464I CTTAAG 1 cut(s) 365
XapI RAATTY 4 cut(s) 220, 242, 511, 536
XmnI GAANNNNTTC 1 cut(s) 526
XspI CTAG 4 cut(s) 95, 185, 341, 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.