MD12G1037800.v1.1

Uncharacterised conserved protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009700
Physical Location & Seq
Forward (+)
4052837 .. 4060668
7832 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1037800.v1.1.491

Sequence Viewer

Length: 2319 bp
ATGTGGCGCTCTCTCTGGCGCTCCATCGACCGCTTCTCGCTCCAGTACTTCAAGTATGTAATCAATGAACTGCGGAAGATCAAAGTTGTGGACGGGCATAACAGGGAACTAGTCATAGATTTATTACAGTCCATAGTGGAGCTAGTTACTTATGGCGATAGACAAGACCCATCGATTTTCGAATATTTTATGGAATACCAAGTTTTATCAGAATTTGTTCGTGTGCTAAAGATCAGTAGAAATTCAAGAATTGAGGCACCGTTGCTTCAGTACCTGAGTATAATGATTCAAAACATGAATAGTGACATTTCAATTTATTATTGTTTTAGCAATGACTACATCAACAATATTATAGGACACAAGTTTGAATTTGGAGGAGATCTAGCGTTATATTATGTTTCCTTTTTGAGAGCAGTGGGCAATAAATTAAACAAAGATACACTTTGCCTTCTTCTGAAGGTTCACGGGGATGCTGTAGCGTCATTTCCCCTGTATGAAGAGGCTCTCAAATTTGCCCACCATGGGGATAAGATGATTCAGACAGCTATTCGTGCATTAAATCTCAGTATATATAATGCTAGTGATGATATGGTTTATCAATATATAACAACTCCTCCAGTCTCAAGGTATTTCTCAGATATGGTTTCAAGCTTAAGCAAGAAGTTCTTTCATCTAGATGCCCTTGTCCACGATGCAGAGATGCGTACACGTCAAAAGAAAGAAGAAATACTTTTGGAAACTGATAAGATAGTCGATGATCTGTATTACTTGAAGGACATACTTGGTATTGGCGAGTCTCGTTTGAGTAGAGCAGTTACTGAAAATCTTCTCAGAATATTAATCGTTCCCATATTACTCCCATTACTGCAGATGGGGCAGAGTAATGGCTCAAATCTGTCTGCAGTTACGTCTTTCTATACTATTTCTTGTCTTCTTCAAGTCATTGGTGGAAAAGGCATATTCAACTTTGTCGCCGAAGTTATTTTATATCCTCATATGACCTCAAGTGTGAGAGATGCTGTTCAAAGGGACTCAACTGAGAGCGATGGTCATGCTAAATCTATATTGAGTGAAATGGGAATAGTATCTTCTAGTCATGAGACTGAAGGAGCAGAAAATAATAGCCTCCTTACTGATACTGTCTCTATCAAAAGGAGTGGAATACTAGCACATATTTTCTCCGAGAATCATAGTCTATTGCTAGCATCACTATTCTTGTTGTTTATTTTAGCAGAAACAAAAGATCTTCATCCTTTGCTGGCTCAAATGACTGGATTAAGTGGAATGCAATATATGATTGATGTGGATGGTAGTATTGGAAATCTCCTATGGAAACATATGGATGTGATTTTAAATGCATTATTGAAGGTTTTAGCAAGTCAACCACCTTACTCTATACTAATACAATGGCATACAGGGTGGTTCTTGCGGAAGCTTTTGATTTGTCAAGGAAAAGGGCTTAGTGATCATAATTTCCAGCTATTCAATACCTCATATCAGCAGTCCCGTGAATGTCTTCAGAAAGAACTTGATGGATGCTGGTTTGATCATATACCAGAGGCTTTCAGAAATGAATGGGCAAACTGTAAAGCAGCTCTTCAGGAAGCATCCCAATGTAAAGATCCTTTTTTTGTTCTTGAGCTTGATGTTAGCCAACAAACTACTGATGGTGATGCAACTTCATACTGTGCTTGGCAGAGGATGGTTGACAATGTGAAGGTTTTTATTCTCCATCTTCAACTTAAATCAACAATTTCCAAAGGAGAATTGCTTGAAGAGCCCTTGCTGGACAGCAGTGCAATTGCTGATTCTAGTAATACGCATGCTTCAAATGGTGCGTCTGCAAGCTTTGGGTCGGAGGTTGGTTTAGGATCTGGAATTTCATGTAGAATTTCATTTTCCAAAGCTGGAATCAGGGATATCTACCTGATACCTGTGGCAAAGAAAACATCTGGAAAAGTGATTCTTGCAGAGAAGCATCCTTTCCGCAGTCAACGAGGAATTGTACTTGCTATTGCACCATTGGCTGGTTTAATTCCAAAAATAGATGAGGATCACCCGACATGGTTGCATCTTCAGATAAGAGAGTTTGAACCAAAACTCTACAAAAGTAGAGTTAGAGGCCATCGTTCGGGGAAGTCCAATCATGTGGCTGATGGGAGATGGACACTTGGTTTTCCAAATGCCAAAGCTTGCGAAGCTGCTCGATTGTCAATACTTGAGGAAACCAGGAAGCAAAGATCCTCTGTCGAGAGTGTACTTGCTCCTCTCCTGCAGAATAGTTGTCTCGGAAATTTATCAGAGAGCGAAGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FPL

Protein Analysis

773

Amino Acids

86.95

Weight (kDa)

6.14

Isoelectric Point (pI)

41.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FPL PF09758 42 - 191 2.1e-46 Uncharacterised conserved protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 256
AccB7I CCANNNNNTGG 1 cut(s) 2027
AciI CCGC 4 cut(s) 31, 73, 1429, 1987
AclWI GGATC 4 cut(s) 1616, 1879, 2061, 2235
AcsI RAATTY 7 cut(s) 212, 241, 368, 509, 1878, 1890, 2293
AcuI CTGAAG 6 cut(s) 251, 476, 1125, 1502, 1583, 2060
AfaI GTAC 5 cut(s) 47, 272, 706, 2007, 2259
AfiI CCNNNNNNNGG 4 cut(s) 522, 523, 2027, 2131
AflII CTTAAG 1 cut(s) 652
AflIII ACRYGT 1 cut(s) 707
AhlI ACTAGT 1 cut(s) 109
AjiI CACGTC 1 cut(s) 710
AjnI CCWGG 1 cut(s) 2228
Alw26I GTCTC 5 cut(s) 625, 801, 1094, 1147, 2291
AlwI GGATC 4 cut(s) 1616, 1879, 2061, 2235
AlwNI CAGNNNCTG 2 cut(s) 274, 818
AoxI GGCC 1 cut(s) 2122
ApeKI GCWGC 2 cut(s) 1592, 2201
ApoI RAATTY 7 cut(s) 212, 241, 368, 509, 1878, 1890, 2293
ArsI GACNNNNNNTTYG 2 cut(s) 1823, 1855
AseI ATTAAT 1 cut(s) 839
Asp700I GAANNNNTTC 3 cut(s) 216, 825, 1515
AspLEI GCGC 2 cut(s) 9, 21
AsuHPI GGTGA 2 cut(s) 1682, 2048
AsuII TTCGAA 1 cut(s) 180
AsuNHI GCTAGC 1 cut(s) 1201
BanI GGYRCC 1 cut(s) 256
BanII GRGCYC 1 cut(s) 1782
BbsI GAAGAC 2 cut(s) 923, 1508
BbvI GCAGC 2 cut(s) 1604, 2188
BcgI CGANNNNNNTGC 4 cut(s) 1034, 1068, 2050, 2084
BciT130I CCWGG 1 cut(s) 2230
BclI TGATCA 2 cut(s) 1465, 1546
BcoDI GTCTC 5 cut(s) 625, 801, 1094, 1147, 2291
BcuI ACTAGT 1 cut(s) 109
BfaI CTAG 9 cut(s) 110, 143, 383, 579, 674, 1092, 1166, 1202, 1812
BfmI CTRYAG 4 cut(s) 474, 866, 900, 2273
BfoI RGCGCY 2 cut(s) 10, 22
BfrI CTTAAG 1 cut(s) 652
BglII AGATCT 2 cut(s) 379, 1243
BisI GCNGC 2 cut(s) 1593, 2202
BlsI GCNGC 2 cut(s) 1594, 2203
BmcAI AGTACT 1 cut(s) 47
Bme1390I CCNGG 1 cut(s) 2230
BmgBI CACGTC 1 cut(s) 710
BmiI GGNNCC 1 cut(s) 258
BmrFI CCNGG 1 cut(s) 2230
BmtI GCTAGC 1 cut(s) 1205
BpiI GAAGAC 2 cut(s) 923, 1508
BpmI CTGGAG 2 cut(s) 26, 600
Bpu14I TTCGAA 1 cut(s) 180
BpuEI CTTGAG 4 cut(s) 607, 988, 1658, 2240
Bsa29I ATCGAT 1 cut(s) 173
BsaBI GATNNNNATC 2 cut(s) 1248, 2052
BsaJI CCNNGG 1 cut(s) 520
BsaXI ACNNNNNCTCC 2 cut(s) 598, 628
Bsc4I CCNNNNNNNGG 4 cut(s) 522, 523, 2027, 2131
Bse1I ACTGG 3 cut(s) 43, 617, 1276
Bse3DI GCAATG 1 cut(s) 337
Bse8I GATNNNNATC 2 cut(s) 1248, 2052
BseBI CCWGG 1 cut(s) 2230
BseCI ATCGAT 1 cut(s) 173
BseDI CCNNGG 1 cut(s) 520
BseGI GGATG 8 cut(s) 475, 1249, 1312, 1348, 1541, 1607, 1707, 1978
BseJI GATNNNNATC 2 cut(s) 1248, 2052
BseLI CCNNNNNNNGG 4 cut(s) 522, 523, 2027, 2131
BseMI GCAATG 1 cut(s) 337
BseMII CTCAG 5 cut(s) 266, 577, 648, 844, 1029
BseNI ACTGG 3 cut(s) 43, 617, 1276
BseRI GAGGAG 3 cut(s) 390, 603, 2256
BseXI GCAGC 2 cut(s) 1604, 2188
Bsh1285I CGRYCG 1 cut(s) 31
BshFI GGCC 1 cut(s) 2124
BshNI GGYRCC 1 cut(s) 256
BshVI ATCGAT 1 cut(s) 173
BsiEI CGRYCG 1 cut(s) 31
BslFI GGGAC 2 cut(s) 1043, 1489
BslI CCNNNNNNNGG 4 cut(s) 522, 523, 2027, 2131
BsmAI GTCTC 5 cut(s) 625, 801, 1094, 1147, 2291
BsmFI GGGAC 2 cut(s) 1043, 1489
BsmI GAATGC 1 cut(s) 1290
BsnI GGCC 1 cut(s) 2124
Bsp119I TTCGAA 1 cut(s) 180
Bsp1286I GDGCHC 1 cut(s) 1782
Bsp19I CCATGG 1 cut(s) 520
BspACI CCGC 4 cut(s) 31, 73, 1429, 1987
BspANI GGCC 1 cut(s) 2124
BspCNI CTCAG 5 cut(s) 267, 576, 647, 843, 1030
BspDI ATCGAT 1 cut(s) 173
BspHI TCATGA 1 cut(s) 1096
BspLI GGNNCC 1 cut(s) 258
BspMAI CTGCAG 3 cut(s) 870, 904, 2277
BspOI GCTAGC 1 cut(s) 1205
BspPI GGATC 4 cut(s) 1616, 1879, 2061, 2235
BspQI GCTCTTC 2 cut(s) 1602, 1770
BspT104I TTCGAA 1 cut(s) 180
BspT107I GGYRCC 1 cut(s) 256
BspTI CTTAAG 1 cut(s) 652
BsrDI GCAATG 1 cut(s) 337
BsrI ACTGG 3 cut(s) 43, 617, 1276
BssECI CCNNGG 1 cut(s) 520
BssT1I CCWWGG 1 cut(s) 520
Bst2UI CCWGG 1 cut(s) 2230
Bst4CI ACNGT 5 cut(s) 129, 261, 1141, 1586, 1688
Bst6I CTCTTC 3 cut(s) 492, 1602, 1770
BstAFI CTTAAG 1 cut(s) 652
BstBI TTCGAA 1 cut(s) 180
BstC8I GCNNGC 5 cut(s) 1203, 1260, 1824, 1846, 2194
BstDEI CTNAG 6 cut(s) 275, 563, 634, 830, 1038, 1460
BstDSI CCRYGG 1 cut(s) 520
BstF5I GGATG 8 cut(s) 475, 1249, 1312, 1348, 1541, 1607, 1707, 1978
BstH2I RGCGCY 2 cut(s) 10, 22
BstHHI GCGC 2 cut(s) 9, 21
BstMAI GTCTC 5 cut(s) 625, 801, 1094, 1147, 2291
BstMCI CGRYCG 1 cut(s) 31
BstMWI GCNNNNNNNGC 5 cut(s) 551, 874, 1777, 2024, 2198
BstNI CCWGG 1 cut(s) 2230
BstNSI RCATGY 1 cut(s) 1826
BstSCI CCNGG 1 cut(s) 2228
BstSFI CTRYAG 4 cut(s) 474, 866, 900, 2273
BstV1I GCAGC 2 cut(s) 1604, 2188
BstV2I GAAGAC 2 cut(s) 923, 1508
BstX2I RGATCY 5 cut(s) 379, 1243, 1621, 1871, 2240
BstXI CCANNNNNNTGG 1 cut(s) 2149
BstYI RGATCY 5 cut(s) 379, 1243, 1621, 1871, 2240
Bsu15I ATCGAT 1 cut(s) 173
BsuRI GGCC 1 cut(s) 2124
BsuTUI ATCGAT 1 cut(s) 173
BtgI CCRYGG 1 cut(s) 520
BtgZI GCGATG 1 cut(s) 1059
BtrI CACGTC 1 cut(s) 710
BtsCI GGATG 8 cut(s) 475, 1249, 1312, 1348, 1541, 1607, 1707, 1978
BtsI GCAGTG 2 cut(s) 420, 1801
BtsIMutI CAGTG 2 cut(s) 420, 1801
Cac8I GCNNGC 5 cut(s) 1203, 1260, 1824, 1846, 2194
CaiI CAGNNNCTG 2 cut(s) 274, 818
CciI TCATGA 1 cut(s) 1096
CfoI GCGC 2 cut(s) 9, 21
ClaI ATCGAT 1 cut(s) 173
CseI GACGC 2 cut(s) 468, 1827
Csp6I GTAC 5 cut(s) 46, 271, 705, 2006, 2258
CspCI CAANNNNNGTGG 2 cut(s) 1138, 1173
CviAII CATG 8 cut(s) 295, 521, 1052, 1097, 1823, 1884, 2064, 2147
CviQI GTAC 5 cut(s) 46, 271, 705, 2006, 2258
DdeI CTNAG 6 cut(s) 275, 563, 634, 830, 1038, 1460
DraI TTTAAA 1 cut(s) 1353
Eam1104I CTCTTC 3 cut(s) 492, 1602, 1770
EarI CTCTTC 3 cut(s) 492, 1602, 1770
Eco130I CCWWGG 1 cut(s) 520
Eco24I GRGCYC 1 cut(s) 1782
Eco32I GATATC 1 cut(s) 1921
Eco57I CTGAAG 6 cut(s) 251, 476, 1125, 1502, 1583, 2060
EcoRII CCWGG 1 cut(s) 2228
EcoRV GATATC 1 cut(s) 1921
EcoT14I CCWWGG 1 cut(s) 520
EcoT22I ATGCAT 1 cut(s) 1360
EcoT38I GRGCYC 1 cut(s) 1782
ErhI CCWWGG 1 cut(s) 520
FaeI CATG 8 cut(s) 298, 524, 1055, 1100, 1826, 1887, 2067, 2150
FaqI GGGAC 2 cut(s) 1043, 1489
FatI CATG 8 cut(s) 294, 520, 1051, 1096, 1822, 1883, 2063, 2146
FauNDI CATATG 2 cut(s) 996, 1338
FbaI TGATCA 2 cut(s) 1465, 1546
Fnu4HI GCNGC 2 cut(s) 1593, 2202
FokI GGATG 8 cut(s) 482, 1236, 1319, 1355, 1548, 1594, 1714, 1965
FriOI GRGCYC 1 cut(s) 1782
Fsp4HI GCNGC 2 cut(s) 1593, 2202
FspBI CTAG 9 cut(s) 110, 143, 383, 579, 674, 1092, 1166, 1202, 1812
GlaI GCGC 2 cut(s) 8, 20
GluI GCNGC 2 cut(s) 1593, 2202
GsuI CTGGAG 2 cut(s) 26, 600
HaeII RGCGCY 2 cut(s) 10, 22
HaeIII GGCC 1 cut(s) 2124
HgaI GACGC 2 cut(s) 468, 1827
HhaI GCGC 2 cut(s) 9, 21
Hin1II CATG 8 cut(s) 298, 524, 1055, 1100, 1826, 1887, 2067, 2150
Hin6I GCGC 2 cut(s) 7, 19
HinP1I GCGC 2 cut(s) 7, 19
HincII GTYRAC 3 cut(s) 1382, 1708, 1994
HindII GTYRAC 3 cut(s) 1382, 1708, 1994
HindIII AAGCTT 4 cut(s) 649, 1433, 1846, 2190
HinfI GANTC 8 cut(s) 286, 535, 794, 1031, 1186, 1808, 1911, 1963
HphI GGTGA 2 cut(s) 1682, 2048
Hpy166II GTNNAC 8 cut(s) 91, 463, 688, 707, 1382, 1708, 1994, 2258
Hpy188III TCNNGA 8 cut(s) 246, 674, 1097, 1601, 1637, 1875, 1953, 2251
Hpy8I GTNNAC 8 cut(s) 91, 463, 688, 707, 1382, 1708, 1994, 2258
HpyAV CCTTC 6 cut(s) 451, 458, 766, 1100, 1360, 1711
HpyCH4III ACNGT 5 cut(s) 129, 261, 1141, 1586, 1688
HpyCH4IV ACGT 2 cut(s) 709, 908
HpyF10VI GCNNNNNNNGC 5 cut(s) 551, 874, 1777, 2024, 2198
HpyF3I CTNAG 6 cut(s) 275, 563, 634, 830, 1038, 1460
HpySE526I ACGT 2 cut(s) 709, 908
Hsp92II CATG 8 cut(s) 298, 524, 1055, 1100, 1826, 1887, 2067, 2150
HspAI GCGC 2 cut(s) 7, 19
Ksp22I TGATCA 2 cut(s) 1465, 1546
LguI GCTCTTC 2 cut(s) 1602, 1770
LmnI GCTCC 5 cut(s) 26, 45, 139, 1109, 2269
Lsp1109I GCAGC 2 cut(s) 1604, 2188
MaeI CTAG 9 cut(s) 110, 143, 383, 579, 674, 1092, 1166, 1202, 1812
MaeII ACGT 2 cut(s) 709, 908
MaeIII GTNAC 4 cut(s) 145, 302, 814, 904
MfeI CAATTG 1 cut(s) 1800
MflI RGATCY 5 cut(s) 379, 1243, 1621, 1871, 2240
MhlI GDGCHC 1 cut(s) 1782
MlyI GAGTC 2 cut(s) 803, 1025
MmeI TCCRAC 1 cut(s) 1836
Mph1103I ATGCAT 1 cut(s) 1360
MroXI GAANNNNTTC 3 cut(s) 216, 825, 1515
MseI TTAA 8 cut(s) 428, 557, 653, 839, 1277, 1352, 1743, 2033
MslI CAYNNNNRTG 4 cut(s) 468, 675, 1341, 1612
MspCI CTTAAG 1 cut(s) 652
MspR9I CCNGG 1 cut(s) 2230
MunI CAATTG 1 cut(s) 1800
Mva1269I GAATGC 1 cut(s) 1290
MvaI CCWGG 1 cut(s) 2230
MwoI GCNNNNNNNGC 5 cut(s) 551, 874, 1777, 2024, 2198
NcoI CCATGG 1 cut(s) 520
NdeI CATATG 2 cut(s) 996, 1338
NheI GCTAGC 1 cut(s) 1201
NlaIII CATG 8 cut(s) 298, 524, 1055, 1100, 1826, 1887, 2067, 2150
NlaIV GGNNCC 1 cut(s) 258
NmuCI GTSAC 1 cut(s) 302
NsiI ATGCAT 1 cut(s) 1360
NspI RCATGY 1 cut(s) 1826
NspV TTCGAA 1 cut(s) 180
PaeI GCATGC 1 cut(s) 1826
PagI TCATGA 1 cut(s) 1096
PciSI GCTCTTC 2 cut(s) 1602, 1770
PctI GAATGC 1 cut(s) 1290
PdmI GAANNNNTTC 3 cut(s) 216, 825, 1515
PfeI GAWTC 6 cut(s) 286, 535, 1186, 1808, 1911, 1963
PflMI CCANNNNNTGG 1 cut(s) 2027
PkrI GCNGC 2 cut(s) 1594, 2203
PleI GAGTC 2 cut(s) 802, 1025
PpsI GAGTC 2 cut(s) 802, 1025
PshBI ATTAAT 1 cut(s) 839
Psp6I CCWGG 1 cut(s) 2228
PspGI CCWGG 1 cut(s) 2228
PspN4I GGNNCC 1 cut(s) 258
PstI CTGCAG 3 cut(s) 870, 904, 2277
PstNI CAGNNNCTG 2 cut(s) 274, 818
PsuI RGATCY 5 cut(s) 379, 1243, 1621, 1871, 2240
RsaI GTAC 5 cut(s) 47, 272, 706, 2007, 2259
RsaNI GTAC 5 cut(s) 46, 271, 705, 2006, 2258
RseI CAYNNNNRTG 4 cut(s) 468, 675, 1341, 1612
SapI GCTCTTC 2 cut(s) 1602, 1770
SaqAI TTAA 8 cut(s) 428, 557, 653, 839, 1277, 1352, 1743, 2033
SatI GCNGC 2 cut(s) 1593, 2202
ScaI AGTACT 1 cut(s) 47
SchI GAGTC 2 cut(s) 803, 1025
ScrFI CCNGG 1 cut(s) 2230
SduI GDGCHC 1 cut(s) 1782
SfcI CTRYAG 4 cut(s) 474, 866, 900, 2273
SfuI TTCGAA 1 cut(s) 180
SmiMI CAYNNNNRTG 4 cut(s) 468, 675, 1341, 1612
SmlI CTYRAG 5 cut(s) 622, 652, 1003, 1637, 2219
SmoI CTYRAG 5 cut(s) 622, 652, 1003, 1637, 2219
SpeI ACTAGT 1 cut(s) 109
SphI GCATGC 1 cut(s) 1826
SsiI CCGC 4 cut(s) 31, 73, 1429, 1987
SspI AATATT 3 cut(s) 185, 349, 837
SspMI CTAG 9 cut(s) 110, 143, 383, 579, 674, 1092, 1166, 1202, 1812
StyD4I CCNGG 1 cut(s) 2228
StyI CCWWGG 1 cut(s) 520
TaaI ACNGT 5 cut(s) 129, 261, 1141, 1586, 1688
TaiI ACGT 2 cut(s) 712, 911
TaqI TCGA 6 cut(s) 27, 173, 180, 753, 2206, 2250
TatI WGTACW 3 cut(s) 45, 2005, 2257
TfiI GAWTC 6 cut(s) 286, 535, 1186, 1808, 1911, 1963
Tru1I TTAA 8 cut(s) 428, 557, 653, 839, 1277, 1352, 1743, 2033
Tru9I TTAA 8 cut(s) 428, 557, 653, 839, 1277, 1352, 1743, 2033
TscAI CASTG 2 cut(s) 420, 1801
TseFI GTSAC 1 cut(s) 302
TseI GCWGC 2 cut(s) 1592, 2201
Tsp45I GTSAC 1 cut(s) 302
TspDTI ATGAA 9 cut(s) 81, 311, 510, 659, 1238, 1587, 1671, 1872, 1884
TspRI CASTG 2 cut(s) 420, 1801
Van91I CCANNNNNTGG 1 cut(s) 2027
Vha464I CTTAAG 1 cut(s) 652
VspI ATTAAT 1 cut(s) 839
XapI RAATTY 7 cut(s) 212, 241, 368, 509, 1878, 1890, 2293
XbaI TCTAGA 1 cut(s) 673
XceI RCATGY 1 cut(s) 1826
XmnI GAANNNNTTC 3 cut(s) 216, 825, 1515
XspI CTAG 9 cut(s) 110, 143, 383, 579, 674, 1092, 1166, 1202, 1812
ZrmI AGTACT 1 cut(s) 47
Zsp2I ATGCAT 1 cut(s) 1360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.