MD12G1105700.v1.1

MATE efflux family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0024422
Physical Location & Seq
Forward (+)
16750272 .. 16750702
431 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1105700.v1.1.491

Sequence Viewer

Length: 285 bp
ATGGTTGGCTTAAGTGGAGCTCTAGAAACCTTGTGCGGCCAGGGGTTTGAAGCAAGGGACTATACGAGGTTAGGAATACATTTGCGGGCCCCTTGCATAGTTTCTCTCCCTTTCACCATCTTCATTGCTATACTTTGGTTCTATGCAGAGCCAATACTTGTTTCTATCCGCCAGGTCAGCGGATTGCAAAATATGCTGCTCTTTATATATCTTTTATTCCAGGATTATTTTCTTATGCTAGTTTGTATTTCTTTTCCTTTATTCAAATGGTTGTTCATGTTGTAA

Protein Analysis

95

Amino Acids

10.86

Weight (kDa)

6.52

Isoelectric Point (pI)

37.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 1 - 58 3.6e-07 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024422)

Species Orthologous Gene IDs
malus_domestica MD12G1105700.v1.1 MD12G1105800.v1.1
pyrus_communis pycom12g07480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 36, 85, 169, 180
AcoI YGGCCR 1 cut(s) 37
AflII CTTAAG 1 cut(s) 10
AgsI TTSAA 2 cut(s) 50, 265
AjnI CCWGG 3 cut(s) 39, 171, 219
AjuI GAANNNNNNNTTGG 2 cut(s) 145, 177
AluBI AGCT 1 cut(s) 20
AluI AGCT 1 cut(s) 20
Alw21I GWGCWC 1 cut(s) 22
AoxI GGCC 2 cut(s) 37, 87
ApaI GGGCCC 1 cut(s) 91
ApeKI GCWGC 1 cut(s) 196
AspS9I GGNCC 2 cut(s) 87, 88
AsuHPI GGTGA 1 cut(s) 106
BaeGI GKGCMC 1 cut(s) 91
BanII GRGCYC 2 cut(s) 22, 91
Bbv12I GWGCWC 1 cut(s) 22
BbvI GCAGC 1 cut(s) 183
BccI CCATC 1 cut(s) 125
BciT130I CCWGG 3 cut(s) 41, 173, 221
BfaI CTAG 2 cut(s) 23, 239
BfrI CTTAAG 1 cut(s) 10
BisI GCNGC 2 cut(s) 37, 197
BlsI GCNGC 2 cut(s) 38, 198
Bme1390I CCNGG 3 cut(s) 41, 173, 221
BmgT120I GGNCC 2 cut(s) 87, 88
BmiI GGNNCC 2 cut(s) 89, 90
BmrFI CCNGG 3 cut(s) 41, 173, 221
BsaJI CCNNGG 1 cut(s) 40
Bse3DI GCAATG 1 cut(s) 123
BseBI CCWGG 3 cut(s) 41, 173, 221
BseDI CCNNGG 1 cut(s) 40
BseMI GCAATG 1 cut(s) 123
BseSI GKGCMC 1 cut(s) 91
BseXI GCAGC 1 cut(s) 183
BshFI GGCC 2 cut(s) 39, 89
BsiHKAI GWGCWC 1 cut(s) 22
BslFI GGGAC 1 cut(s) 71
BsmFI GGGAC 1 cut(s) 71
BsnI GGCC 2 cut(s) 39, 89
Bsp120I GGGCCC 1 cut(s) 87
Bsp1286I GDGCHC 2 cut(s) 22, 91
BspACI CCGC 4 cut(s) 36, 85, 169, 180
BspANI GGCC 2 cut(s) 39, 89
BspLI GGNNCC 2 cut(s) 89, 90
BspTI CTTAAG 1 cut(s) 10
BsrDI GCAATG 1 cut(s) 123
BssECI CCNNGG 1 cut(s) 40
Bst2UI CCWGG 3 cut(s) 41, 173, 221
BstAFI CTTAAG 1 cut(s) 10
BstAPI GCANNNNNTGC 1 cut(s) 193
BstC8I GCNNGC 1 cut(s) 87
BstMWI GCNNNNNNNGC 2 cut(s) 177, 193
BstNI CCWGG 3 cut(s) 41, 173, 221
BstSCI CCNGG 3 cut(s) 39, 171, 219
BstSLI GKGCMC 1 cut(s) 91
BstV1I GCAGC 1 cut(s) 183
BsuRI GGCC 2 cut(s) 39, 89
Cac8I GCNNGC 1 cut(s) 87
Cfr13I GGNCC 2 cut(s) 87, 88
CviAII CATG 1 cut(s) 277
CviJI RGCY 5 cut(s) 9, 20, 39, 89, 151
CviKI_1 RGCY 5 cut(s) 9, 20, 39, 89, 151
EaeI YGGCCR 1 cut(s) 37
EciI GGCGGA 1 cut(s) 158
Ecl136II GAGCTC 1 cut(s) 20
Eco24I GRGCYC 2 cut(s) 22, 91
Eco53kI GAGCTC 1 cut(s) 20
EcoICRI GAGCTC 1 cut(s) 20
EcoO109I RGGNCCY 1 cut(s) 88
EcoRII CCWGG 3 cut(s) 39, 171, 219
EcoT38I GRGCYC 2 cut(s) 22, 91
FaeI CATG 1 cut(s) 280
FaiI YATR 9 cut(s) 63, 98, 131, 144, 194, 206, 208, 236, 278
FaqI GGGAC 1 cut(s) 71
FatI CATG 1 cut(s) 276
FauI CCCGC 1 cut(s) 78
Fnu4HI GCNGC 2 cut(s) 37, 197
FriOI GRGCYC 2 cut(s) 22, 91
Fsp4HI GCNGC 2 cut(s) 37, 197
FspBI CTAG 2 cut(s) 23, 239
GluI GCNGC 2 cut(s) 37, 197
HaeIII GGCC 2 cut(s) 39, 89
Hin1II CATG 1 cut(s) 280
HphI GGTGA 1 cut(s) 106
Hpy188III TCNNGA 1 cut(s) 23
HpyCH4V TGCA 3 cut(s) 96, 146, 187
HpyF10VI GCNNNNNNNGC 2 cut(s) 177, 193
Hsp92II CATG 1 cut(s) 280
LmnI GCTCC 1 cut(s) 17
LpnPI CCDG 6 cut(s) 26, 53, 158, 185, 206, 233
Lsp1109I GCAGC 1 cut(s) 183
MaeI CTAG 2 cut(s) 23, 239
MboII GAAGA 1 cut(s) 112
MhlI GDGCHC 2 cut(s) 22, 91
MnlI CCTC 1 cut(s) 60
MseI TTAA 1 cut(s) 11
MspA1I CMGCKG 1 cut(s) 180
MspCI CTTAAG 1 cut(s) 10
MspR9I CCNGG 3 cut(s) 41, 173, 221
MvaI CCWGG 3 cut(s) 41, 173, 221
MwoI GCNNNNNNNGC 2 cut(s) 177, 193
NlaIII CATG 1 cut(s) 280
NlaIV GGNNCC 2 cut(s) 89, 90
PfoI TCCNGGA 1 cut(s) 219
PkrI GCNGC 2 cut(s) 38, 198
Psp124BI GAGCTC 1 cut(s) 22
Psp6I CCWGG 3 cut(s) 39, 171, 219
PspGI CCWGG 3 cut(s) 39, 171, 219
PspN4I GGNNCC 2 cut(s) 89, 90
PspOMI GGGCCC 1 cut(s) 87
PspPI GGNCC 2 cut(s) 87, 88
SacI GAGCTC 1 cut(s) 22
SaqAI TTAA 1 cut(s) 11
SatI GCNGC 2 cut(s) 37, 197
Sau96I GGNCC 2 cut(s) 87, 88
ScrFI CCNGG 3 cut(s) 41, 173, 221
SduI GDGCHC 2 cut(s) 22, 91
SetI ASST 4 cut(s) 22, 32, 71, 177
SmlI CTYRAG 1 cut(s) 10
SmoI CTYRAG 1 cut(s) 10
SsiI CCGC 4 cut(s) 36, 85, 169, 180
SspMI CTAG 2 cut(s) 23, 239
SstI GAGCTC 1 cut(s) 22
StyD4I CCNGG 3 cut(s) 39, 171, 219
TauI GCSGC 1 cut(s) 39
Tru1I TTAA 1 cut(s) 11
Tru9I TTAA 1 cut(s) 11
TseI GCWGC 1 cut(s) 196
TspDTI ATGAA 2 cut(s) 112, 265
Vha464I CTTAAG 1 cut(s) 10
XbaI TCTAGA 1 cut(s) 22
XspI CTAG 2 cut(s) 23, 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.