pycom12g07480

MATE efflux family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0024422
Physical Location & Seq
Forward (+)
7837021 .. 7837788
768 bp
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UTR
Exon/CDS
Intron
pycom12g07480.1

Sequence Viewer

Length: 636 bp
ATGTTTGCCGCGCACCTTGGTGAGCTCGAGCTTGCCGGAGCCATACTGGCTAACGCGTGGGCAACCACTACTGGTTTTGTTGTTATGGTTGGCTTAAGTGGAGCTCTAGAAACCTTGTGCGGCCAGGGGTTTGAAGCAAGGGACTATAGGATGTTAGGAATACATTTGCAGGCCTCTTGCATAATTTCTCTCCCTTTCACCATCTTCATAGCTATACTTTGGCTCTATATAGAGCCAATACTTATTTCTATTCGCCAGGATCGAGCAGTTGCAAAATATGCCGCTCTTTATATCAAATATTTTATTCCAGGATTATTTCCTTATGCTATTTTACAAAATATATTAAGATTTATGCAGGCACAATCAGTTGTCTTACCCCTCTATTTCTTTTCCGTTATGCAAATGGTTGTTCATGTTGTAATAACGTATAGTCTAGTACATTGGACAACTCTTCGTTACAAGGGAGCTTCTCTAGCAGCTTTAATATCAATATGGATATCGATGCTAATGTTAGTGGTGTACGTTATGTGTGCAAAGAAGTTCAAGCGTACATGGGGTGGATTTTCTTTAGAATCATTCCACTGTGTTCTTACTAATTTGAAACTCGCTTTCCGCACTCCATTAATGGTATGGTAA

Protein Analysis

212

Amino Acids

23.85

Weight (kDa)

9.36

Isoelectric Point (pI)

29.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 1 - 143 5.7e-23 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024422)

Species Orthologous Gene IDs
malus_domestica MD12G1105700.v1.1 MD12G1105800.v1.1
pyrus_communis pycom12g07480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 284
AccII CGCG 2 cut(s) 11, 56
AciI CCGC 4 cut(s) 9, 120, 282, 613
AclWI GGATC 1 cut(s) 267
AcoI YGGCCR 1 cut(s) 121
AfaI GTAC 3 cut(s) 438, 521, 550
AflII CTTAAG 1 cut(s) 94
AflIII ACRYGT 1 cut(s) 54
AgsI TTSAA 3 cut(s) 134, 544, 601
AjnI CCWGG 3 cut(s) 123, 255, 307
AjuI GAANNNNNNNTTGG 2 cut(s) 229, 261
AleI CACNNNNGTG 1 cut(s) 18
AluBI AGCT 6 cut(s) 25, 31, 104, 212, 467, 479
AluI AGCT 6 cut(s) 25, 31, 104, 212, 467, 479
Alw21I GWGCWC 2 cut(s) 27, 106
AlwI GGATC 1 cut(s) 267
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 2 cut(s) 121, 171
ApeKI GCWGC 1 cut(s) 476
AseI ATTAAT 1 cut(s) 623
AspLEI GCGC 1 cut(s) 13
AsuHPI GGTGA 2 cut(s) 32, 190
AvaI CYCGRG 1 cut(s) 26
BanII GRGCYC 2 cut(s) 27, 106
Bbv12I GWGCWC 2 cut(s) 27, 106
BbvI GCAGC 1 cut(s) 488
BccI CCATC 1 cut(s) 209
BciT130I CCWGG 3 cut(s) 125, 257, 309
BfaI CTAG 3 cut(s) 107, 434, 473
BfmI CTRYAG 1 cut(s) 145
BfrI CTTAAG 1 cut(s) 94
BglI GCCNNNNNGGC 1 cut(s) 47
BisI GCNGC 4 cut(s) 9, 121, 282, 477
BlsI GCNGC 4 cut(s) 10, 122, 283, 478
Bme1390I CCNGG 3 cut(s) 125, 257, 309
BmeT110I CYCGRG 1 cut(s) 26
BmiI GGNNCC 1 cut(s) 40
BmrFI CCNGG 3 cut(s) 125, 257, 309
BmsI GCATC 1 cut(s) 492
Bsa29I ATCGAT 1 cut(s) 500
BsaJI CCNNGG 2 cut(s) 16, 124
Bse1I ACTGG 2 cut(s) 51, 76
BseBI CCWGG 3 cut(s) 125, 257, 309
BseCI ATCGAT 1 cut(s) 500
BseDI CCNNGG 2 cut(s) 16, 124
BseGI GGATG 1 cut(s) 156
BseNI ACTGG 2 cut(s) 51, 76
BseXI GCAGC 1 cut(s) 488
Bsh1236I CGCG 2 cut(s) 11, 56
BshFI GGCC 2 cut(s) 123, 173
BshVI ATCGAT 1 cut(s) 500
BsiHKAI GWGCWC 2 cut(s) 27, 106
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 1 cut(s) 36
BslFI GGGAC 1 cut(s) 155
BsmFI GGGAC 1 cut(s) 155
BsnI GGCC 2 cut(s) 123, 173
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 2 cut(s) 27, 106
Bsp143I GATC 1 cut(s) 259
BspACI CCGC 4 cut(s) 9, 120, 282, 613
BspANI GGCC 2 cut(s) 123, 173
BspDI ATCGAT 1 cut(s) 500
BspFNI CGCG 2 cut(s) 11, 56
BspLI GGNNCC 1 cut(s) 40
BspPI GGATC 1 cut(s) 267
BspTI CTTAAG 1 cut(s) 94
BsrBI CCGCTC 1 cut(s) 284
BsrI ACTGG 2 cut(s) 51, 76
BssECI CCNNGG 2 cut(s) 16, 124
BssMI GATC 1 cut(s) 259
BssT1I CCWWGG 1 cut(s) 16
Bst2UI CCWGG 3 cut(s) 125, 257, 309
Bst4CI ACNGT 1 cut(s) 584
Bst6I CTCTTC 1 cut(s) 456
BstAFI CTTAAG 1 cut(s) 94
BstAPI GCANNNNNTGC 1 cut(s) 278
BstC8I GCNNGC 3 cut(s) 33, 171, 357
BstF5I GGATG 1 cut(s) 156
BstFNI CGCG 2 cut(s) 11, 56
BstHHI GCGC 1 cut(s) 13
BstKTI GATC 1 cut(s) 262
BstMBI GATC 1 cut(s) 259
BstMWI GCNNNNNNNGC 3 cut(s) 47, 278, 473
BstNI CCWGG 3 cut(s) 125, 257, 309
BstSCI CCNGG 3 cut(s) 123, 255, 307
BstSFI CTRYAG 1 cut(s) 145
BstUI CGCG 2 cut(s) 11, 56
BstV1I GCAGC 1 cut(s) 488
Bsu15I ATCGAT 1 cut(s) 500
BsuRI GGCC 2 cut(s) 123, 173
BsuTUI ATCGAT 1 cut(s) 500
BtsCI GGATG 1 cut(s) 156
BtsIMutI CAGTG 1 cut(s) 580
Cac8I GCNNGC 3 cut(s) 33, 171, 357
CfoI GCGC 1 cut(s) 13
ClaI ATCGAT 1 cut(s) 500
Csp6I GTAC 3 cut(s) 437, 520, 549
CviAII CATG 2 cut(s) 413, 552
CviQI GTAC 3 cut(s) 437, 520, 549
DpnI GATC 1 cut(s) 261
DpnII GATC 1 cut(s) 259
EaeI YGGCCR 1 cut(s) 121
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
Ecl136II GAGCTC 2 cut(s) 25, 104
Eco130I CCWWGG 1 cut(s) 16
Eco147I AGGCCT 1 cut(s) 173
Eco24I GRGCYC 2 cut(s) 27, 106
Eco32I GATATC 1 cut(s) 498
Eco53kI GAGCTC 2 cut(s) 25, 104
Eco88I CYCGRG 1 cut(s) 26
EcoICRI GAGCTC 2 cut(s) 25, 104
EcoRII CCWGG 3 cut(s) 123, 255, 307
EcoRV GATATC 1 cut(s) 498
EcoT14I CCWWGG 1 cut(s) 16
EcoT38I GRGCYC 2 cut(s) 27, 106
ErhI CCWWGG 1 cut(s) 16
FaeI CATG 2 cut(s) 416, 555
FaqI GGGAC 1 cut(s) 155
FatI CATG 2 cut(s) 412, 551
Fnu4HI GCNGC 4 cut(s) 9, 121, 282, 477
FokI GGATG 1 cut(s) 163
FriOI GRGCYC 2 cut(s) 27, 106
Fsp4HI GCNGC 4 cut(s) 9, 121, 282, 477
FspBI CTAG 3 cut(s) 107, 434, 473
GlaI GCGC 1 cut(s) 12
GluI GCNGC 4 cut(s) 9, 121, 282, 477
HaeIII GGCC 2 cut(s) 123, 173
HapII CCGG 1 cut(s) 36
HhaI GCGC 1 cut(s) 13
Hin1II CATG 2 cut(s) 416, 555
Hin6I GCGC 1 cut(s) 11
HinP1I GCGC 1 cut(s) 11
HinfI GANTC 1 cut(s) 572
HpaII CCGG 1 cut(s) 36
HphI GGTGA 2 cut(s) 32, 190
Hpy166II GTNNAC 1 cut(s) 520
Hpy188III TCNNGA 1 cut(s) 107
Hpy8I GTNNAC 1 cut(s) 520
HpyCH4III ACNGT 1 cut(s) 584
HpyCH4IV ACGT 2 cut(s) 425, 522
HpyCH4V TGCA 6 cut(s) 169, 180, 272, 355, 400, 533
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 278, 473
HpySE526I ACGT 2 cut(s) 425, 522
Hsp92II CATG 2 cut(s) 416, 555
HspAI GCGC 1 cut(s) 11
Kzo9I GATC 1 cut(s) 259
LmnI GCTCC 3 cut(s) 38, 101, 464
Lsp1109I GCAGC 1 cut(s) 488
LweI GCATC 1 cut(s) 492
MaeI CTAG 3 cut(s) 107, 434, 473
MaeII ACGT 2 cut(s) 425, 522
MaeIII GTNAC 1 cut(s) 455
MalI GATC 1 cut(s) 261
MbiI CCGCTC 1 cut(s) 284
MboI GATC 1 cut(s) 259
MboII GAAGA 2 cut(s) 196, 443
MhlI GDGCHC 2 cut(s) 27, 106
MluCI AATT 2 cut(s) 183, 595
MluI ACGCGT 1 cut(s) 54
MnlI CCTC 2 cut(s) 184, 389
MseI TTAA 4 cut(s) 95, 344, 482, 623
MslI CAYNNNNRTG 1 cut(s) 18
MspCI CTTAAG 1 cut(s) 94
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 3 cut(s) 125, 257, 309
MvaI CCWGG 3 cut(s) 125, 257, 309
MvnI CGCG 2 cut(s) 11, 56
MwoI GCNNNNNNNGC 3 cut(s) 47, 278, 473
NdeII GATC 1 cut(s) 259
NlaIII CATG 2 cut(s) 416, 555
NlaIV GGNNCC 1 cut(s) 40
OliI CACNNNNGTG 1 cut(s) 18
PaeR7I CTCGAG 1 cut(s) 26
PceI AGGCCT 1 cut(s) 173
PcsI WCGNNNNNNNCGW 1 cut(s) 259
PfeI GAWTC 1 cut(s) 572
PfoI TCCNGGA 1 cut(s) 307
PkrI GCNGC 4 cut(s) 10, 122, 283, 478
PshBI ATTAAT 1 cut(s) 623
Psp124BI GAGCTC 2 cut(s) 27, 106
Psp6I CCWGG 3 cut(s) 123, 255, 307
PspGI CCWGG 3 cut(s) 123, 255, 307
PspN4I GGNNCC 1 cut(s) 40
PspXI VCTCGAGB 1 cut(s) 26
RsaI GTAC 3 cut(s) 438, 521, 550
RsaNI GTAC 3 cut(s) 437, 520, 549
RseI CAYNNNNRTG 1 cut(s) 18
SacI GAGCTC 2 cut(s) 27, 106
SaqAI TTAA 4 cut(s) 95, 344, 482, 623
SatI GCNGC 4 cut(s) 9, 121, 282, 477
Sau3AI GATC 1 cut(s) 259
ScrFI CCNGG 3 cut(s) 125, 257, 309
SduI GDGCHC 2 cut(s) 27, 106
SfaNI GCATC 1 cut(s) 492
SfcI CTRYAG 1 cut(s) 145
Sfr274I CTCGAG 1 cut(s) 26
SlaI CTCGAG 1 cut(s) 26
SmiMI CAYNNNNRTG 1 cut(s) 18
SmlI CTYRAG 2 cut(s) 26, 94
SmoI CTYRAG 2 cut(s) 26, 94
Sse9I AATT 2 cut(s) 183, 595
SseBI AGGCCT 1 cut(s) 173
SsiI CCGC 4 cut(s) 9, 120, 282, 613
SspI AATATT 1 cut(s) 299
SspMI CTAG 3 cut(s) 107, 434, 473
SstI GAGCTC 2 cut(s) 27, 106
StuI AGGCCT 1 cut(s) 173
StyD4I CCNGG 3 cut(s) 123, 255, 307
StyI CCWWGG 1 cut(s) 16
TaaI ACNGT 1 cut(s) 584
TaiI ACGT 2 cut(s) 428, 525
TaqI TCGA 3 cut(s) 27, 262, 500
TasI AATT 2 cut(s) 183, 595
TatI WGTACW 1 cut(s) 436
TauI GCSGC 3 cut(s) 11, 123, 284
TfiI GAWTC 1 cut(s) 572
Tru1I TTAA 4 cut(s) 95, 344, 482, 623
Tru9I TTAA 4 cut(s) 95, 344, 482, 623
TscAI CASTG 1 cut(s) 587
TseI GCWGC 1 cut(s) 476
TspDTI ATGAA 2 cut(s) 196, 401
TspGWI ACGGA 1 cut(s) 382
TspRI CASTG 1 cut(s) 587
Vha464I CTTAAG 1 cut(s) 94
VspI ATTAAT 1 cut(s) 623
XbaI TCTAGA 1 cut(s) 106
XcmI CCANNNNNNNNNTGG 1 cut(s) 627
XhoI CTCGAG 1 cut(s) 26
XspI CTAG 3 cut(s) 107, 434, 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.