MD12G1175700.v1.1

EG45-like domain containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0024416
Physical Location & Seq
Reverse (-)
25617992 .. 25619031
1040 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1175700.v1.1.491

Sequence Viewer

Length: 396 bp
ATGGAACTCTTATTCATGTTAATGGTTTTAAGCTTTTTTTGCACAGAATTAAGGCTAGTTTCTGGCGATCTCGGGACTGCAACTCCATACAGTCCTCCTTATATTCCAACAAGATGCTTTGGGACTAGGCCAGACCAGTTCCCTCCGATGTACCTTTTTTTGGCGGTGAGTGAAGGGTTGTGGGACGGTGGGTCTGCGTGCGGACGGATCTATAAAATTAGGTGCCTGAGTGGACCTAATATGCCCTGCAAGGGTCGTGCTACCGTGGAGGCGAAGGTGGTGGATTTTTGCCGTCAATCTCCATGCCCTTCTACCATAGCCATGTCAACCGATGCTTTTGCAGCCATCTCTCACAATTCTTCTGCACCAATTAACATCGAATATGCCCAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1

Protein Analysis

132

Amino Acids

14.33

Weight (kDa)

6.53

Isoelectric Point (pI)

58.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 55 - 128 1.2e-07 Lytic transglycolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0024416)

Species Orthologous Gene IDs
malus_domestica MD12G1175700.v1.1 MD12G1176400.v1.1
pyrus_communis pycom12g16610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 222
AciI CCGC 2 cut(s) 164, 201
AclWI GGATC 1 cut(s) 215
AfaI GTAC 1 cut(s) 152
AfiI CCNNNNNNNGG 2 cut(s) 160, 251
AhdI GACNNNNNGTC 1 cut(s) 190
AluBI AGCT 1 cut(s) 33
AluI AGCT 1 cut(s) 33
AlwI GGATC 1 cut(s) 215
Ama87I CYCGRG 1 cut(s) 71
AoxI GGCC 1 cut(s) 128
ApeKI GCWGC 1 cut(s) 341
AspS9I GGNCC 1 cut(s) 233
AsuHPI GGTGA 1 cut(s) 178
AvaI CYCGRG 1 cut(s) 71
AvaII GGWCC 1 cut(s) 233
BanI GGYRCC 1 cut(s) 222
BbvI GCAGC 1 cut(s) 353
BccI CCATC 1 cut(s) 353
BceAI ACGGC 1 cut(s) 276
BcgI CGANNNNNNTGC 2 cut(s) 320, 354
BfaI CTAG 2 cut(s) 56, 126
BisI GCNGC 1 cut(s) 342
BlsI GCNGC 1 cut(s) 343
Bme18I GGWCC 1 cut(s) 233
BmeRI GACNNNNNGTC 1 cut(s) 190
BmeT110I CYCGRG 1 cut(s) 71
BmgT120I GGNCC 1 cut(s) 233
BmiI GGNNCC 1 cut(s) 224
BmsI GCATC 2 cut(s) 104, 322
BsaJI CCNNGG 1 cut(s) 264
BsaXI ACNNNNNCTCC 2 cut(s) 67, 97
Bsc4I CCNNNNNNNGG 2 cut(s) 160, 251
Bse1I ACTGG 1 cut(s) 136
BseDI CCNNGG 1 cut(s) 264
BseLI CCNNNNNNNGG 2 cut(s) 160, 251
BseMII CTCAG 1 cut(s) 218
BseNI ACTGG 1 cut(s) 136
BseXI GCAGC 1 cut(s) 353
BsgI GTGCAG 1 cut(s) 348
BshFI GGCC 1 cut(s) 130
BshNI GGYRCC 1 cut(s) 222
BsiHKCI CYCGRG 1 cut(s) 71
BslFI GGGAC 3 cut(s) 88, 136, 197
BslI CCNNNNNNNGG 2 cut(s) 160, 251
BsmFI GGGAC 3 cut(s) 88, 136, 197
BsnI GGCC 1 cut(s) 130
BsoBI CYCGRG 1 cut(s) 71
Bsp143I GATC 2 cut(s) 67, 207
BspACI CCGC 2 cut(s) 164, 201
BspANI GGCC 1 cut(s) 130
BspCNI CTCAG 1 cut(s) 219
BspLI GGNNCC 1 cut(s) 224
BspPI GGATC 1 cut(s) 215
BspT107I GGYRCC 1 cut(s) 222
BsrI ACTGG 1 cut(s) 136
BssECI CCNNGG 1 cut(s) 264
BssMI GATC 2 cut(s) 67, 207
Bst4CI ACNGT 3 cut(s) 92, 188, 265
BstC8I GCNNGC 1 cut(s) 199
BstDEI CTNAG 1 cut(s) 227
BstDSI CCRYGG 1 cut(s) 264
BstKTI GATC 2 cut(s) 70, 210
BstMBI GATC 2 cut(s) 67, 207
BstMWI GCNNNNNNNGC 2 cut(s) 39, 341
BstV1I GCAGC 1 cut(s) 353
BstX2I RGATCY 1 cut(s) 207
BstYI RGATCY 1 cut(s) 207
BsuRI GGCC 1 cut(s) 130
BtgI CCRYGG 1 cut(s) 264
Cac8I GCNNGC 1 cut(s) 199
Cfr13I GGNCC 1 cut(s) 233
Csp6I GTAC 1 cut(s) 151
CviAII CATG 3 cut(s) 16, 303, 322
CviJI RGCY 5 cut(s) 33, 55, 130, 320, 344
CviKI_1 RGCY 5 cut(s) 33, 55, 130, 320, 344
CviQI GTAC 1 cut(s) 151
DdeI CTNAG 1 cut(s) 227
DpnI GATC 2 cut(s) 69, 209
DpnII GATC 2 cut(s) 67, 207
DriI GACNNNNNGTC 1 cut(s) 190
Eam1105I GACNNNNNGTC 1 cut(s) 190
Eco47I GGWCC 1 cut(s) 233
Eco88I CYCGRG 1 cut(s) 71
FaeI CATG 3 cut(s) 19, 306, 325
FaqI GGGAC 3 cut(s) 88, 136, 197
FatI CATG 3 cut(s) 15, 302, 321
Fnu4HI GCNGC 1 cut(s) 342
Fsp4HI GCNGC 1 cut(s) 342
FspBI CTAG 2 cut(s) 56, 126
GluI GCNGC 1 cut(s) 342
HaeIII GGCC 1 cut(s) 130
Hin1II CATG 3 cut(s) 19, 306, 325
HincII GTYRAC 1 cut(s) 327
HindII GTYRAC 1 cut(s) 327
HindIII AAGCTT 1 cut(s) 31
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 2 cut(s) 233, 327
Hpy188I TCNGA 1 cut(s) 147
Hpy188III TCNNGA 1 cut(s) 73
Hpy8I GTNNAC 2 cut(s) 233, 327
HpyAV CCTTC 3 cut(s) 167, 268, 318
HpyCH4III ACNGT 3 cut(s) 92, 188, 265
HpyCH4V TGCA 5 cut(s) 42, 80, 249, 341, 365
HpyF10VI GCNNNNNNNGC 2 cut(s) 39, 341
HpyF3I CTNAG 1 cut(s) 227
Hsp92II CATG 3 cut(s) 19, 306, 325
Kzo9I GATC 2 cut(s) 67, 207
LpnPI CCDG 5 cut(s) 48, 144, 149, 239, 259
Lsp1109I GCAGC 1 cut(s) 353
LweI GCATC 2 cut(s) 104, 322
MaeI CTAG 2 cut(s) 56, 126
MalI GATC 2 cut(s) 69, 209
MboI GATC 2 cut(s) 67, 207
MboII GAAGA 1 cut(s) 351
MflI RGATCY 1 cut(s) 207
MluCI AATT 4 cut(s) 47, 216, 355, 369
MmeI TCCRAC 1 cut(s) 131
MnlI CCTC 3 cut(s) 105, 153, 262
MseI TTAA 4 cut(s) 20, 29, 50, 372
MslI CAYNNNNRTG 2 cut(s) 20, 320
MwoI GCNNNNNNNGC 2 cut(s) 39, 341
NdeII GATC 2 cut(s) 67, 207
NlaIII CATG 3 cut(s) 19, 306, 325
NlaIV GGNNCC 1 cut(s) 224
PkrI GCNGC 1 cut(s) 343
PspN4I GGNNCC 1 cut(s) 224
PspPI GGNCC 1 cut(s) 233
PsuI RGATCY 1 cut(s) 207
RsaI GTAC 1 cut(s) 152
RsaNI GTAC 1 cut(s) 151
RseI CAYNNNNRTG 2 cut(s) 20, 320
SaqAI TTAA 4 cut(s) 20, 29, 50, 372
SatI GCNGC 1 cut(s) 342
Sau3AI GATC 2 cut(s) 67, 207
Sau96I GGNCC 1 cut(s) 233
SetI ASST 5 cut(s) 35, 156, 224, 238, 279
SfaNI GCATC 2 cut(s) 104, 322
SinI GGWCC 1 cut(s) 233
SmiMI CAYNNNNRTG 2 cut(s) 20, 320
Sse9I AATT 4 cut(s) 47, 216, 355, 369
SsiI CCGC 2 cut(s) 164, 201
SspMI CTAG 2 cut(s) 56, 126
TaaI ACNGT 3 cut(s) 92, 188, 265
TaqI TCGA 1 cut(s) 378
TasI AATT 4 cut(s) 47, 216, 355, 369
Tru1I TTAA 4 cut(s) 20, 29, 50, 372
Tru9I TTAA 4 cut(s) 20, 29, 50, 372
TseI GCWGC 1 cut(s) 341
TspDTI ATGAA 1 cut(s) 4
TspGWI ACGGA 1 cut(s) 220
VpaK11BI GGWCC 1 cut(s) 233
XspI CTAG 2 cut(s) 56, 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.