pycom12g16610

EG45-like domain containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0024416
Physical Location & Seq
Reverse (-)
18966092 .. 18966916
825 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g16610.2

Sequence Viewer

Length: 459 bp
ATGGCCGCTCCTTTGACAACATCTTGGGCGGGCTCTGTTAACATGGGACTCTTATTCATGTTGATCGTTTTAGGCTTTTTTTGCACAGAATCAAGGCTAGTTTCTGGCGATCTCGGCACAGCAAATTCTTATGGTCCTCCTTATATGCCAACAAGATGCTTTGGGAGTAGGCCAGACCAGTTCCCTCCGATGTACCTTTTTGTGGCGGTGAGTGAAGGGTTGTGGGACGGTGGTTCTGCGTGTGGAAGGCTCTATAAAATTAGGTGCCTGAGTGGACCTAATATGCCCTGCAAGGGTGGCGCTATTGTGGAGGTGAAGGTGGTGGACCTTTGCCGTCAATCTCCTTGCCCTTCTACCATAGCCATGTCAACCGATGCTTTTGCTGCCATCTCACACTCACCTTCTGCACGAATCAACATCGAATACGTGCAGAAATCAGCTGAACCAGATCTATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1

Protein Analysis

153

Amino Acids

16.24

Weight (kDa)

6.53

Isoelectric Point (pI)

57.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024416)

Species Orthologous Gene IDs
malus_domestica MD12G1175700.v1.1 MD12G1176400.v1.1
pyrus_communis pycom12g16610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 264
AccBSI CCGCTC 1 cut(s) 8
AciI CCGC 3 cut(s) 6, 29, 206
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 124
AfaI GTAC 1 cut(s) 194
AfiI CCNNNNNNNGG 2 cut(s) 202, 293
AluBI AGCT 1 cut(s) 440
AluI AGCT 1 cut(s) 440
AoxI GGCC 2 cut(s) 3, 170
ApeKI GCWGC 1 cut(s) 383
ApoI RAATTY 1 cut(s) 124
AspLEI GCGC 1 cut(s) 302
AspS9I GGNCC 3 cut(s) 134, 275, 325
AsuHPI GGTGA 3 cut(s) 220, 325, 390
AvaII GGWCC 3 cut(s) 134, 275, 325
BanI GGYRCC 1 cut(s) 264
BanII GRGCYC 1 cut(s) 35
BbvI GCAGC 1 cut(s) 370
BccI CCATC 1 cut(s) 395
BceAI ACGGC 1 cut(s) 318
BcgI CGANNNNNNTGC 2 cut(s) 362, 396
BfaI CTAG 1 cut(s) 98
BfoI RGCGCY 1 cut(s) 303
BglII AGATCT 1 cut(s) 448
BisI GCNGC 2 cut(s) 6, 384
BlsI GCNGC 2 cut(s) 7, 385
Bme18I GGWCC 3 cut(s) 134, 275, 325
BmgT120I GGNCC 3 cut(s) 134, 275, 325
BmiI GGNNCC 1 cut(s) 266
BmsI GCATC 2 cut(s) 146, 364
BsaAI YACGTR 1 cut(s) 427
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 293
Bse1I ACTGG 1 cut(s) 178
BseLI CCNNNNNNNGG 2 cut(s) 202, 293
BseMII CTCAG 1 cut(s) 260
BseNI ACTGG 1 cut(s) 178
BseXI GCAGC 1 cut(s) 370
BsgI GTGCAG 2 cut(s) 390, 449
BshFI GGCC 2 cut(s) 5, 172
BshNI GGYRCC 1 cut(s) 264
BslFI GGGAC 2 cut(s) 60, 239
BslI CCNNNNNNNGG 2 cut(s) 202, 293
BsmFI GGGAC 2 cut(s) 60, 239
BsnI GGCC 2 cut(s) 5, 172
Bsp1286I GDGCHC 1 cut(s) 35
Bsp143I GATC 3 cut(s) 63, 109, 448
BspACI CCGC 3 cut(s) 6, 29, 206
BspANI GGCC 2 cut(s) 5, 172
BspCNI CTCAG 1 cut(s) 261
BspLI GGNNCC 1 cut(s) 266
BspT107I GGYRCC 1 cut(s) 264
BsrBI CCGCTC 1 cut(s) 8
BsrI ACTGG 1 cut(s) 178
BssMI GATC 3 cut(s) 63, 109, 448
Bst4CI ACNGT 1 cut(s) 230
BstBAI YACGTR 1 cut(s) 427
BstC8I GCNNGC 1 cut(s) 31
BstDEI CTNAG 1 cut(s) 269
BstH2I RGCGCY 1 cut(s) 303
BstHHI GCGC 1 cut(s) 302
BstKTI GATC 3 cut(s) 66, 112, 451
BstMBI GATC 3 cut(s) 63, 109, 448
BstMWI GCNNNNNNNGC 4 cut(s) 81, 114, 297, 383
BstV1I GCAGC 1 cut(s) 370
BstX2I RGATCY 1 cut(s) 448
BstYI RGATCY 1 cut(s) 448
BsuRI GGCC 2 cut(s) 5, 172
Cac8I GCNNGC 1 cut(s) 31
CfoI GCGC 1 cut(s) 302
Cfr13I GGNCC 3 cut(s) 134, 275, 325
Csp6I GTAC 1 cut(s) 193
CviAII CATG 3 cut(s) 43, 58, 364
CviJI RGCY 8 cut(s) 5, 33, 75, 97, 172, 250, 362, 440
CviKI_1 RGCY 8 cut(s) 5, 33, 75, 97, 172, 250, 362, 440
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 1 cut(s) 269
DpnI GATC 3 cut(s) 65, 111, 450
DpnII GATC 3 cut(s) 63, 109, 448
EaeI YGGCCR 1 cut(s) 3
Eco24I GRGCYC 1 cut(s) 35
Eco47I GGWCC 3 cut(s) 134, 275, 325
EcoT38I GRGCYC 1 cut(s) 35
FaeI CATG 3 cut(s) 46, 61, 367
FaqI GGGAC 2 cut(s) 60, 239
FatI CATG 3 cut(s) 42, 57, 363
FauI CCCGC 1 cut(s) 22
Fnu4HI GCNGC 2 cut(s) 6, 384
FriOI GRGCYC 1 cut(s) 35
Fsp4HI GCNGC 2 cut(s) 6, 384
FspBI CTAG 1 cut(s) 98
GlaI GCGC 1 cut(s) 301
GluI GCNGC 2 cut(s) 6, 384
HaeII RGCGCY 1 cut(s) 303
HaeIII GGCC 2 cut(s) 5, 172
HhaI GCGC 1 cut(s) 302
Hin1II CATG 3 cut(s) 46, 61, 367
Hin6I GCGC 1 cut(s) 300
HinP1I GCGC 1 cut(s) 300
HincII GTYRAC 2 cut(s) 40, 369
HindII GTYRAC 2 cut(s) 40, 369
HinfI GANTC 3 cut(s) 48, 89, 411
HpaI GTTAAC 1 cut(s) 40
HphI GGTGA 3 cut(s) 220, 325, 390
Hpy166II GTNNAC 4 cut(s) 40, 275, 325, 369
Hpy188I TCNGA 1 cut(s) 189
Hpy8I GTNNAC 4 cut(s) 40, 275, 325, 369
HpyAV CCTTC 5 cut(s) 209, 240, 310, 360, 411
HpyCH4III ACNGT 1 cut(s) 230
HpyCH4IV ACGT 1 cut(s) 426
HpyCH4V TGCA 4 cut(s) 84, 291, 407, 430
HpyF10VI GCNNNNNNNGC 4 cut(s) 81, 114, 297, 383
HpyF3I CTNAG 1 cut(s) 269
HpySE526I ACGT 1 cut(s) 426
Hsp92II CATG 3 cut(s) 46, 61, 367
HspAI GCGC 1 cut(s) 300
KspAI GTTAAC 1 cut(s) 40
Kzo9I GATC 3 cut(s) 63, 109, 448
LmnI GCTCC 1 cut(s) 13
LpnPI CCDG 5 cut(s) 90, 186, 191, 281, 301
Lsp1109I GCAGC 1 cut(s) 370
LweI GCATC 2 cut(s) 146, 364
MaeI CTAG 1 cut(s) 98
MaeII ACGT 1 cut(s) 426
MalI GATC 3 cut(s) 65, 111, 450
MbiI CCGCTC 1 cut(s) 8
MboI GATC 3 cut(s) 63, 109, 448
MflI RGATCY 1 cut(s) 448
MhlI GDGCHC 1 cut(s) 35
MluCI AATT 2 cut(s) 124, 258
MlyI GAGTC 1 cut(s) 42
MnlI CCTC 3 cut(s) 147, 195, 304
MseI TTAA 1 cut(s) 39
MslI CAYNNNNRTG 1 cut(s) 362
MspA1I CMGCKG 1 cut(s) 440
MwoI GCNNNNNNNGC 4 cut(s) 81, 114, 297, 383
NdeII GATC 3 cut(s) 63, 109, 448
NlaIII CATG 3 cut(s) 46, 61, 367
NlaIV GGNNCC 1 cut(s) 266
NmeAIII GCCGAG 1 cut(s) 93
PfeI GAWTC 2 cut(s) 89, 411
PkrI GCNGC 2 cut(s) 7, 385
PleI GAGTC 1 cut(s) 42
PpsI GAGTC 1 cut(s) 42
Ppu21I YACGTR 1 cut(s) 427
PspN4I GGNNCC 1 cut(s) 266
PspPI GGNCC 3 cut(s) 134, 275, 325
PsuI RGATCY 1 cut(s) 448
PvuII CAGCTG 1 cut(s) 440
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
RseI CAYNNNNRTG 1 cut(s) 362
SaqAI TTAA 1 cut(s) 39
SatI GCNGC 2 cut(s) 6, 384
Sau3AI GATC 3 cut(s) 63, 109, 448
Sau96I GGNCC 3 cut(s) 134, 275, 325
SchI GAGTC 1 cut(s) 42
SduI GDGCHC 1 cut(s) 35
SetI ASST 9 cut(s) 198, 266, 280, 315, 321, 330, 403, 429, 442
SfaNI GCATC 2 cut(s) 146, 364
SinI GGWCC 3 cut(s) 134, 275, 325
SmiMI CAYNNNNRTG 1 cut(s) 362
Sse9I AATT 2 cut(s) 124, 258
SsiI CCGC 3 cut(s) 6, 29, 206
SspMI CTAG 1 cut(s) 98
TaaI ACNGT 1 cut(s) 230
TaiI ACGT 1 cut(s) 429
TaqI TCGA 1 cut(s) 420
TasI AATT 2 cut(s) 124, 258
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 2 cut(s) 89, 411
Tru1I TTAA 1 cut(s) 39
Tru9I TTAA 1 cut(s) 39
TseI GCWGC 1 cut(s) 383
TspDTI ATGAA 1 cut(s) 46
VpaK11BI GGWCC 3 cut(s) 134, 275, 325
XapI RAATTY 1 cut(s) 124
XspI CTAG 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.