MD12G1176400.v1.1

EG45-like domain containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0024416
Physical Location & Seq
Reverse (-)
25665460 .. 25666398
939 bp
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UTR
Exon/CDS
Intron
MD12G1176400.v1.1.491

Sequence Viewer

Length: 396 bp
ATGAGCCTCTTAGTCCTGCCAGTGGTGTTATGCTTACTCTGCACAGTACCTAGGCCAGTTTCTGCCGATCTGGGAACTGCAACTTCATACGGCCCTCCTTATATACCTACAAGATGCTTTGGGAGGAGGCCTGACCAGTTCCCTCCGCAGTACTTTTTTGTGGCCGTGAGTGAAGGATTGTGGGACGGTGGTTCTGCGTGCGGAAGGGTCTATAAAATTAAATGCCTCAGTGGACTTAATATGCCCTGCAAGGTTGGTGAGACTGTCGAGGTGATGGTGGTGGACTTTTGCCGGAAATCTCCTTGCCCTTCCACCATAGCCATGTCAACCTATGCTTTTGCAGCCATCTCTCACAATTCTTCTGCACCAATCAACATCGAATATCTCCAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1

Protein Analysis

132

Amino Acids

14.2

Weight (kDa)

8.13

Isoelectric Point (pI)

63.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 55 - 128 9.8e-10 Lytic transglycolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0024416)

Species Orthologous Gene IDs
malus_domestica MD12G1175700.v1.1 MD12G1176400.v1.1
pyrus_communis pycom12g16610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 146, 201
AcoI YGGCCR 1 cut(s) 162
AfaI GTAC 2 cut(s) 48, 152
AfiI CCNNNNNNNGG 1 cut(s) 22
Alw26I GTCTC 1 cut(s) 254
AlwNI CAGNNNCTG 1 cut(s) 62
AoxI GGCC 4 cut(s) 53, 91, 128, 162
ApeKI GCWGC 1 cut(s) 341
AspA2I CCTAGG 1 cut(s) 50
AspS9I GGNCC 1 cut(s) 92
AsuHPI GGTGA 2 cut(s) 269, 283
AvrII CCTAGG 1 cut(s) 50
BbvI GCAGC 1 cut(s) 353
BccI CCATC 2 cut(s) 268, 353
BceAI ACGGC 2 cut(s) 106, 149
BcoDI GTCTC 1 cut(s) 254
BfaI CTAG 1 cut(s) 51
BisI GCNGC 1 cut(s) 342
BlnI CCTAGG 1 cut(s) 50
BlsI GCNGC 1 cut(s) 343
BmcAI AGTACT 1 cut(s) 152
BmgT120I GGNCC 1 cut(s) 92
BmsI GCATC 1 cut(s) 104
BpmI CTGGAG 1 cut(s) 371
BsaJI CCNNGG 1 cut(s) 50
Bsc4I CCNNNNNNNGG 1 cut(s) 22
Bse1I ACTGG 3 cut(s) 20, 56, 136
BseDI CCNNGG 1 cut(s) 50
BseLI CCNNNNNNNGG 1 cut(s) 22
BseMII CTCAG 1 cut(s) 241
BseNI ACTGG 3 cut(s) 20, 56, 136
BseRI GAGGAG 1 cut(s) 139
BseXI GCAGC 1 cut(s) 353
BsgI GTGCAG 2 cut(s) 25, 348
BshFI GGCC 4 cut(s) 55, 93, 130, 164
BsiSI CCGG 1 cut(s) 292
BslFI GGGAC 1 cut(s) 197
BslI CCNNNNNNNGG 1 cut(s) 22
BsmAI GTCTC 1 cut(s) 254
BsmFI GGGAC 1 cut(s) 197
BsnI GGCC 4 cut(s) 55, 93, 130, 164
Bsp143I GATC 1 cut(s) 67
BspACI CCGC 2 cut(s) 146, 201
BspANI GGCC 4 cut(s) 55, 93, 130, 164
BspCNI CTCAG 1 cut(s) 240
BsrI ACTGG 3 cut(s) 20, 56, 136
BssECI CCNNGG 1 cut(s) 50
BssMI GATC 1 cut(s) 67
BssT1I CCWWGG 1 cut(s) 50
Bst4CI ACNGT 3 cut(s) 46, 188, 265
BstC8I GCNNGC 1 cut(s) 199
BstDEI CTNAG 2 cut(s) 10, 227
BstKTI GATC 1 cut(s) 70
BstMAI GTCTC 1 cut(s) 254
BstMBI GATC 1 cut(s) 67
BstMWI GCNNNNNNNGC 2 cut(s) 39, 341
BstV1I GCAGC 1 cut(s) 353
BsuRI GGCC 4 cut(s) 55, 93, 130, 164
BtsIMutI CAGTG 2 cut(s) 27, 235
Cac8I GCNNGC 1 cut(s) 199
CaiI CAGNNNCTG 1 cut(s) 62
Cfr13I GGNCC 1 cut(s) 92
Csp6I GTAC 2 cut(s) 47, 151
CviAII CATG 1 cut(s) 322
CviJI RGCY 7 cut(s) 6, 55, 93, 130, 164, 320, 344
CviKI_1 RGCY 7 cut(s) 6, 55, 93, 130, 164, 320, 344
CviQI GTAC 2 cut(s) 47, 151
DdeI CTNAG 2 cut(s) 10, 227
DpnI GATC 1 cut(s) 69
DpnII GATC 1 cut(s) 67
EaeI YGGCCR 1 cut(s) 162
Eco130I CCWWGG 1 cut(s) 50
Eco147I AGGCCT 1 cut(s) 130
EcoT14I CCWWGG 1 cut(s) 50
ErhI CCWWGG 1 cut(s) 50
FaeI CATG 1 cut(s) 325
FaqI GGGAC 1 cut(s) 197
FatI CATG 1 cut(s) 321
Fnu4HI GCNGC 1 cut(s) 342
Fsp4HI GCNGC 1 cut(s) 342
FspBI CTAG 1 cut(s) 51
GluI GCNGC 1 cut(s) 342
GsuI CTGGAG 1 cut(s) 371
HaeIII GGCC 4 cut(s) 55, 93, 130, 164
HapII CCGG 1 cut(s) 292
Hin1II CATG 1 cut(s) 325
HincII GTYRAC 1 cut(s) 327
HindII GTYRAC 1 cut(s) 327
HpaII CCGG 1 cut(s) 292
HphI GGTGA 2 cut(s) 269, 283
Hpy166II GTNNAC 3 cut(s) 233, 283, 327
Hpy188III TCNNGA 1 cut(s) 388
Hpy8I GTNNAC 3 cut(s) 233, 283, 327
HpyAV CCTTC 3 cut(s) 167, 198, 318
HpyCH4III ACNGT 3 cut(s) 46, 188, 265
HpyCH4V TGCA 5 cut(s) 42, 80, 249, 341, 365
HpyF10VI GCNNNNNNNGC 2 cut(s) 39, 341
HpyF3I CTNAG 2 cut(s) 10, 227
Hsp92II CATG 1 cut(s) 325
Kzo9I GATC 1 cut(s) 67
LpnPI CCDG 8 cut(s) 29, 33, 56, 69, 144, 149, 259, 305
Lsp1109I GCAGC 1 cut(s) 353
LweI GCATC 1 cut(s) 104
MaeI CTAG 1 cut(s) 51
MalI GATC 1 cut(s) 69
MboI GATC 1 cut(s) 67
MboII GAAGA 1 cut(s) 351
MluCI AATT 2 cut(s) 216, 355
MnlI CCTC 7 cut(s) 17, 105, 117, 120, 153, 236, 262
MseI TTAA 2 cut(s) 219, 237
MslI CAYNNNNRTG 1 cut(s) 320
MspI CCGG 1 cut(s) 292
MwoI GCNNNNNNNGC 2 cut(s) 39, 341
NdeII GATC 1 cut(s) 67
NlaIII CATG 1 cut(s) 325
PceI AGGCCT 1 cut(s) 130
PkrI GCNGC 1 cut(s) 343
PspPI GGNCC 1 cut(s) 92
PstNI CAGNNNCTG 1 cut(s) 62
RsaI GTAC 2 cut(s) 48, 152
RsaNI GTAC 2 cut(s) 47, 151
RseI CAYNNNNRTG 1 cut(s) 320
SaqAI TTAA 2 cut(s) 219, 237
SatI GCNGC 1 cut(s) 342
Sau3AI GATC 1 cut(s) 67
Sau96I GGNCC 1 cut(s) 92
ScaI AGTACT 1 cut(s) 152
SetI ASST 5 cut(s) 52, 109, 255, 273, 332
SfaNI GCATC 1 cut(s) 104
SmiMI CAYNNNNRTG 1 cut(s) 320
Sse9I AATT 2 cut(s) 216, 355
SseBI AGGCCT 1 cut(s) 130
SsiI CCGC 2 cut(s) 146, 201
SspMI CTAG 1 cut(s) 51
StuI AGGCCT 1 cut(s) 130
StyI CCWWGG 1 cut(s) 50
TaaI ACNGT 3 cut(s) 46, 188, 265
TaqI TCGA 2 cut(s) 267, 378
TasI AATT 2 cut(s) 216, 355
TatI WGTACW 1 cut(s) 150
Tru1I TTAA 2 cut(s) 219, 237
Tru9I TTAA 2 cut(s) 219, 237
TscAI CASTG 2 cut(s) 27, 235
TseI GCWGC 1 cut(s) 341
TspDTI ATGAA 1 cut(s) 75
TspRI CASTG 2 cut(s) 27, 235
XmaJI CCTAGG 1 cut(s) 50
XspI CTAG 1 cut(s) 51
ZrmI AGTACT 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.