MD13G1053500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0023917
Physical Location & Seq
Reverse (-)
3775170 .. 3775984
815 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1053500.v1.1.491

Sequence Viewer

Length: 441 bp
ATGACACGTGCAACTGCAAGAACATCACAAAGCACACCATCCCAAAAACTGGAAATCCCCTCTCCCAAATTCCCAGAAAATTTCTTTGTATTTTTGCTGCCGTTCTTGCCTTCTTTCTTCTCCTTTATTTTTTCCTTTGCTGCGTCCTCACCTCCTCAGCTTCTGTATAAATGTCGTCGGAGCAACGCGATCCTCTCACCAGCTGAAATTGGAAAAGAAAAAAAAAACCCCAATTCGGATCTTCCCGTTTGCCAATCAGGAAATAGAAAGATGTTGATGATGGGATTGAGCATCCAGAGCTTACTGGATTTGGCCGTGGCCGGAATTTCAATGATGGTCGGGTTGGGGATCTTCGCGTTTATCGCTTCCATTCTTTGCTCTGCCGCGTTCCTGCACACCGCCAGCGCCAAGGACGTTTACCGTAGCATACAGAAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

147

Amino Acids

16.0

Weight (kDa)

9.67

Isoelectric Point (pI)

58.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023917)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68877
malus_domestica MD13G1053500.v1.1
prunus_persica Prupe.1G298600_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 49
AccII CGCG 3 cut(s) 188, 356, 386
AciI CCGC 2 cut(s) 384, 399
AclWI GGATC 3 cut(s) 184, 246, 356
AcoI YGGCCR 2 cut(s) 312, 318
AcsI RAATTY 3 cut(s) 68, 79, 324
AcvI CACGTG 1 cut(s) 8
AfiI CCNNNNNNNGG 2 cut(s) 49, 235
AflIII ACRYGT 1 cut(s) 5
AgsI TTSAA 1 cut(s) 330
AluBI AGCT 3 cut(s) 160, 203, 300
AluI AGCT 3 cut(s) 160, 203, 300
AlwI GGATC 3 cut(s) 184, 246, 356
AlwNI CAGNNNCTG 1 cut(s) 163
AoxI GGCC 2 cut(s) 312, 318
ApeKI GCWGC 2 cut(s) 97, 140
ApoI RAATTY 3 cut(s) 68, 79, 324
AspLEI GCGC 1 cut(s) 407
AsuHPI GGTGA 2 cut(s) 141, 189
BbrPI CACGTG 1 cut(s) 8
BbvCI CCTCAGC 1 cut(s) 156
BbvI GCAGC 2 cut(s) 84, 127
BccI CCATC 3 cut(s) 46, 274, 328
BceAI ACGGC 2 cut(s) 85, 299
BfoI RGCGCY 1 cut(s) 408
BisI GCNGC 3 cut(s) 98, 141, 384
BlsI GCNGC 3 cut(s) 99, 142, 385
BmsI GCATC 1 cut(s) 300
Bpu10I CCTNAGC 1 cut(s) 156
BsaAI YACGTR 1 cut(s) 8
BsaJI CCNNGG 2 cut(s) 315, 408
Bsc4I CCNNNNNNNGG 2 cut(s) 49, 235
Bse1I ACTGG 2 cut(s) 54, 309
BseDI CCNNGG 2 cut(s) 315, 408
BseGI GGATG 2 cut(s) 38, 291
BseLI CCNNNNNNNGG 2 cut(s) 49, 235
BseMII CTCAG 1 cut(s) 170
BseNI ACTGG 2 cut(s) 54, 309
BseRI GAGGAG 1 cut(s) 144
BseXI GCAGC 2 cut(s) 84, 127
BsgI GTGCAG 1 cut(s) 377
Bsh1236I CGCG 3 cut(s) 188, 356, 386
BshFI GGCC 2 cut(s) 314, 320
BsiSI CCGG 1 cut(s) 321
BslI CCNNNNNNNGG 2 cut(s) 49, 235
BsnI GGCC 2 cut(s) 314, 320
Bsp143I GATC 3 cut(s) 189, 238, 348
BspACI CCGC 2 cut(s) 384, 399
BspANI GGCC 2 cut(s) 314, 320
BspCNI CTCAG 1 cut(s) 169
BspFNI CGCG 3 cut(s) 188, 356, 386
BspPI GGATC 3 cut(s) 184, 246, 356
BsrI ACTGG 2 cut(s) 54, 309
BssECI CCNNGG 2 cut(s) 315, 408
BssMI GATC 3 cut(s) 189, 238, 348
BssT1I CCWWGG 1 cut(s) 408
Bst4CI ACNGT 1 cut(s) 422
BstBAI YACGTR 1 cut(s) 8
BstC8I GCNNGC 1 cut(s) 403
BstDEI CTNAG 1 cut(s) 156
BstDSI CCRYGG 1 cut(s) 315
BstF5I GGATG 2 cut(s) 38, 291
BstFNI CGCG 3 cut(s) 188, 356, 386
BstH2I RGCGCY 1 cut(s) 408
BstHHI GCGC 1 cut(s) 407
BstKTI GATC 3 cut(s) 192, 241, 351
BstMBI GATC 3 cut(s) 189, 238, 348
BstMWI GCNNNNNNNGC 3 cut(s) 106, 297, 362
BstUI CGCG 3 cut(s) 188, 356, 386
BstV1I GCAGC 2 cut(s) 84, 127
BstX2I RGATCY 2 cut(s) 238, 348
BstYI RGATCY 2 cut(s) 238, 348
BsuRI GGCC 2 cut(s) 314, 320
BtgI CCRYGG 1 cut(s) 315
BtsCI GGATG 2 cut(s) 38, 291
Cac8I GCNNGC 1 cut(s) 403
CaiI CAGNNNCTG 1 cut(s) 163
CfoI GCGC 1 cut(s) 407
CseI GACGC 1 cut(s) 132
CviJI RGCY 5 cut(s) 160, 203, 300, 314, 320
CviKI_1 RGCY 5 cut(s) 160, 203, 300, 314, 320
DdeI CTNAG 1 cut(s) 156
DpnI GATC 3 cut(s) 191, 240, 350
DpnII GATC 3 cut(s) 189, 238, 348
EaeI YGGCCR 2 cut(s) 312, 318
Eco130I CCWWGG 1 cut(s) 408
Eco72I CACGTG 1 cut(s) 8
EcoT14I CCWWGG 1 cut(s) 408
ErhI CCWWGG 1 cut(s) 408
FaiI YATR 2 cut(s) 168, 428
Fnu4HI GCNGC 3 cut(s) 98, 141, 384
FokI GGATG 2 cut(s) 25, 278
Fsp4HI GCNGC 3 cut(s) 98, 141, 384
GlaI GCGC 1 cut(s) 406
GluI GCNGC 3 cut(s) 98, 141, 384
HaeII RGCGCY 1 cut(s) 408
HaeIII GGCC 2 cut(s) 314, 320
HapII CCGG 1 cut(s) 321
HgaI GACGC 1 cut(s) 132
HhaI GCGC 1 cut(s) 407
Hin6I GCGC 1 cut(s) 405
HinP1I GCGC 1 cut(s) 405
HpaII CCGG 1 cut(s) 321
HphI GGTGA 2 cut(s) 141, 189
Hpy166II GTNNAC 1 cut(s) 418
Hpy188I TCNGA 2 cut(s) 180, 238
Hpy188III TCNNGA 2 cut(s) 258, 295
Hpy8I GTNNAC 1 cut(s) 418
Hpy99I CGWCG 1 cut(s) 180
HpyAV CCTTC 2 cut(s) 120, 427
HpyCH4III ACNGT 1 cut(s) 422
HpyCH4IV ACGT 2 cut(s) 7, 414
HpyCH4V TGCA 3 cut(s) 11, 17, 394
HpyF10VI GCNNNNNNNGC 3 cut(s) 106, 297, 362
HpyF3I CTNAG 1 cut(s) 156
HpySE526I ACGT 2 cut(s) 7, 414
HspAI GCGC 1 cut(s) 405
Kzo9I GATC 3 cut(s) 189, 238, 348
LmnI GCTCC 1 cut(s) 180
LpnPI CCDG 9 cut(s) 35, 87, 213, 243, 290, 308, 334, 404, 415
Lsp1109I GCAGC 2 cut(s) 84, 127
LweI GCATC 1 cut(s) 300
MaeII ACGT 2 cut(s) 7, 414
MalI GATC 3 cut(s) 191, 240, 350
MboI GATC 3 cut(s) 189, 238, 348
MboII GAAGA 3 cut(s) 109, 233, 343
MflI RGATCY 2 cut(s) 238, 348
MluCI AATT 5 cut(s) 68, 79, 207, 232, 324
MmeI TCCRAC 1 cut(s) 158
MnlI CCTC 5 cut(s) 70, 157, 162, 165, 203
MspA1I CMGCKG 1 cut(s) 203
MspI CCGG 1 cut(s) 321
MvnI CGCG 3 cut(s) 188, 356, 386
MwoI GCNNNNNNNGC 3 cut(s) 106, 297, 362
NdeII GATC 3 cut(s) 189, 238, 348
PflMI CCANNNNNTGG 1 cut(s) 49
PkrI GCNGC 3 cut(s) 99, 142, 385
PmaCI CACGTG 1 cut(s) 8
PmlI CACGTG 1 cut(s) 8
Ppu21I YACGTR 1 cut(s) 8
PspCI CACGTG 1 cut(s) 8
PstNI CAGNNNCTG 1 cut(s) 163
PsuI RGATCY 2 cut(s) 238, 348
PvuII CAGCTG 1 cut(s) 203
SatI GCNGC 3 cut(s) 98, 141, 384
Sau3AI GATC 3 cut(s) 189, 238, 348
SetI ASST 6 cut(s) 10, 154, 162, 205, 302, 417
SfaNI GCATC 1 cut(s) 300
Sse9I AATT 5 cut(s) 68, 79, 207, 232, 324
SsiI CCGC 2 cut(s) 384, 399
StyI CCWWGG 1 cut(s) 408
TaaI ACNGT 1 cut(s) 422
TaiI ACGT 2 cut(s) 10, 417
TasI AATT 5 cut(s) 68, 79, 207, 232, 324
TauI GCSGC 1 cut(s) 386
TseI GCWGC 2 cut(s) 97, 140
Van91I CCANNNNNTGG 1 cut(s) 49
XapI RAATTY 3 cut(s) 68, 79, 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.