MD13G1061300.v1.1

Component of the coenzyme Q biosynthetic pathway. May play a role in organizing a multi-subunit COQ enzyme complex required for coenzyme Q biosynthesis. Required for steady-state levels of other COQ polypeptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0020356
Physical Location & Seq
Forward (+)
4308053 .. 4309251
1199 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1061300.v1.1.491

Sequence Viewer

Length: 501 bp
ATGTTAGGAAGTGCGCGTGTTCGGCTGAACCGGTGGCAGCAGGCGGCTGTGGCGGTCGGTTCGGCTGTGGGCGCATTGCTGGACCCAAGAAGAGCTGATCTCATAGCAGCTCTTGGAGAGACAACTGGGAAACCTGCATTTGAAAGAGTTCTTGAAAGAATGACGAGGAGCCCTGAAGGCAGGGCCATACTGTTGGAGAAACCCCGTGTAATATCTGTAAACGTGGGTCATGCATGGGATCTACCAGCGAACACATTTGGTGCTGCCTATGCAAAGTTCATGGGATCTAGGAACTTCTCGCCAGATGATCGACCACCAGTGCGGTTCATGGAGACAGATGAGCTTGCATATGTGGCCATGAGGGCTCGCGAGGTGCATGACTTTTGGCACACCCTTTTTGGCCTTCCAACGAACTTGATGGGGGAGTCGGCCCTCAAGGTAATAGAGTTTGAGCAAATGTACCTTCCCATGTGCCTGATGTCCGTCATAGGGGCTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Coq4

Protein Analysis

167

Amino Acids

18.43

Weight (kDa)

7.94

Isoelectric Point (pI)

39.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Coq4 PF05019 6 - 164 7.1e-61 Coenzyme Q (ubiquinone) biosynthesis protein Coq4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020356)

Species Orthologous Gene IDs
malus_domestica MD13G1061300.v1.1
rosa_chinensis RchiOBHm_Chr7g0182561
rosa_laevigata RLG00000001441 RLG00000001445
rosa_multiflora Rmu_sc0005811.1_g000004
rosa_samantha Rh7AG051900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 142
AccII CGCG 2 cut(s) 16, 369
AciI CCGC 3 cut(s) 44, 53, 322
AclWI GGATC 2 cut(s) 246, 292
AcoI YGGCCR 1 cut(s) 354
AcuI CTGAAG 1 cut(s) 195
AfaI GTAC 1 cut(s) 461
AfiI CCNNNNNNNGG 1 cut(s) 489
AgeI ACCGGT 1 cut(s) 30
AgsI TTSAA 2 cut(s) 143, 155
AluBI AGCT 3 cut(s) 95, 110, 343
AluI AGCT 3 cut(s) 95, 110, 343
Alw26I GTCTC 2 cut(s) 113, 326
AlwI GGATC 2 cut(s) 246, 292
AoxI GGCC 4 cut(s) 183, 354, 400, 429
ApeKI GCWGC 3 cut(s) 37, 107, 263
AsiGI ACCGGT 1 cut(s) 30
Asp700I GAANNNNTTC 1 cut(s) 147
AspLEI GCGC 2 cut(s) 16, 74
AspS9I GGNCC 3 cut(s) 82, 183, 430
AvaII GGWCC 1 cut(s) 82
BalI TGGCCA 1 cut(s) 356
BanII GRGCYC 3 cut(s) 173, 367, 496
BbvI GCAGC 3 cut(s) 49, 119, 250
BccI CCATC 1 cut(s) 412
BcoDI GTCTC 2 cut(s) 113, 326
BfaI CTAG 1 cut(s) 288
BfuAI ACCTGC 1 cut(s) 142
BglI GCCNNNNNGGC 2 cut(s) 177, 362
BisI GCNGC 4 cut(s) 38, 45, 108, 264
BlsI GCNGC 4 cut(s) 39, 46, 109, 265
Bme18I GGWCC 1 cut(s) 82
BmgT120I GGNCC 3 cut(s) 82, 183, 430
BmiI GGNNCC 2 cut(s) 84, 170
BmrI ACTGGG 1 cut(s) 135
BmuI ACTGGG 1 cut(s) 135
BplI GAGNNNNNCTC 2 cut(s) 84, 116
BpuEI CTTGAG 1 cut(s) 419
BsaWI WCCGGW 1 cut(s) 30
Bsc4I CCNNNNNNNGG 1 cut(s) 489
Bse118I RCCGGY 1 cut(s) 30
Bse1I ACTGG 2 cut(s) 130, 317
Bse3DI GCAATG 1 cut(s) 74
BseLI CCNNNNNNNGG 1 cut(s) 489
BseMI GCAATG 1 cut(s) 74
BseNI ACTGG 2 cut(s) 130, 317
BseRI GAGGAG 1 cut(s) 181
BseXI GCAGC 3 cut(s) 49, 119, 250
Bsh1236I CGCG 2 cut(s) 16, 369
Bsh1285I CGRYCG 1 cut(s) 57
BshFI GGCC 4 cut(s) 185, 356, 402, 431
BshTI ACCGGT 1 cut(s) 30
BsiEI CGRYCG 1 cut(s) 57
BsiSI CCGG 1 cut(s) 31
BslI CCNNNNNNNGG 1 cut(s) 489
BsmAI GTCTC 2 cut(s) 113, 326
BsnI GGCC 4 cut(s) 185, 356, 402, 431
Bsp1286I GDGCHC 3 cut(s) 173, 367, 496
Bsp143I GATC 4 cut(s) 97, 238, 284, 307
Bsp68I TCGCGA 1 cut(s) 369
BspACI CCGC 3 cut(s) 44, 53, 322
BspANI GGCC 4 cut(s) 185, 356, 402, 431
BspFNI CGCG 2 cut(s) 16, 369
BspLI GGNNCC 2 cut(s) 84, 170
BspMI ACCTGC 1 cut(s) 142
BspPI GGATC 2 cut(s) 246, 292
BspQI GCTCTTC 1 cut(s) 85
BsrDI GCAATG 1 cut(s) 74
BsrFI RCCGGY 1 cut(s) 30
BsrI ACTGG 2 cut(s) 130, 317
BssAI RCCGGY 1 cut(s) 30
BssMI GATC 4 cut(s) 97, 238, 284, 307
Bst4CI ACNGT 1 cut(s) 192
Bst6I CTCTTC 1 cut(s) 85
BstC8I GCNNGC 3 cut(s) 42, 345, 367
BstDEI CTNAG 1 cut(s) 495
BstFNI CGCG 2 cut(s) 16, 369
BstHHI GCGC 2 cut(s) 16, 74
BstKTI GATC 4 cut(s) 100, 241, 287, 310
BstMAI GTCTC 2 cut(s) 113, 326
BstMBI GATC 4 cut(s) 97, 238, 284, 307
BstMCI CGRYCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 7 cut(s) 22, 50, 71, 177, 269, 353, 362
BstUI CGCG 2 cut(s) 16, 369
BstV1I GCAGC 3 cut(s) 49, 119, 250
BstX2I RGATCY 2 cut(s) 238, 284
BstXI CCANNNNNNTGG 1 cut(s) 193
BstYI RGATCY 2 cut(s) 238, 284
BsuRI GGCC 4 cut(s) 185, 356, 402, 431
BtsIMutI CAGTG 1 cut(s) 324
BtuMI TCGCGA 1 cut(s) 369
BveI ACCTGC 1 cut(s) 142
Cac8I GCNNGC 3 cut(s) 42, 345, 367
CfoI GCGC 2 cut(s) 16, 74
Cfr10I RCCGGY 1 cut(s) 30
Cfr13I GGNCC 3 cut(s) 82, 183, 430
Csp6I GTAC 1 cut(s) 460
CspAI ACCGGT 1 cut(s) 30
CviAII CATG 7 cut(s) 230, 234, 280, 328, 358, 377, 469
CviQI GTAC 1 cut(s) 460
DdeI CTNAG 1 cut(s) 495
DpnI GATC 4 cut(s) 99, 240, 286, 309
DpnII GATC 4 cut(s) 97, 238, 284, 307
EaeI YGGCCR 1 cut(s) 354
Eam1104I CTCTTC 1 cut(s) 85
EarI CTCTTC 1 cut(s) 85
Eco24I GRGCYC 3 cut(s) 173, 367, 496
Eco47I GGWCC 1 cut(s) 82
Eco57I CTGAAG 1 cut(s) 195
EcoT22I ATGCAT 1 cut(s) 235
EcoT38I GRGCYC 3 cut(s) 173, 367, 496
FaeI CATG 7 cut(s) 233, 237, 283, 331, 361, 380, 472
FatI CATG 7 cut(s) 229, 233, 279, 327, 357, 376, 468
FauNDI CATATG 1 cut(s) 349
Fnu4HI GCNGC 4 cut(s) 38, 45, 108, 264
FriOI GRGCYC 3 cut(s) 173, 367, 496
Fsp4HI GCNGC 4 cut(s) 38, 45, 108, 264
FspBI CTAG 1 cut(s) 288
GlaI GCGC 2 cut(s) 15, 73
GluI GCNGC 4 cut(s) 38, 45, 108, 264
HaeIII GGCC 4 cut(s) 185, 356, 402, 431
HapII CCGG 1 cut(s) 31
HhaI GCGC 2 cut(s) 16, 74
Hin1II CATG 7 cut(s) 233, 237, 283, 331, 361, 380, 472
Hin6I GCGC 2 cut(s) 14, 72
HinP1I GCGC 2 cut(s) 14, 72
HinfI GANTC 1 cut(s) 425
HpaII CCGG 1 cut(s) 31
Hpy166II GTNNAC 1 cut(s) 220
Hpy188III TCNNGA 2 cut(s) 152, 368
Hpy8I GTNNAC 1 cut(s) 220
HpyAV CCTTC 3 cut(s) 170, 413, 473
HpyCH4III ACNGT 1 cut(s) 192
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 5 cut(s) 137, 233, 272, 347, 376
HpyF10VI GCNNNNNNNGC 7 cut(s) 22, 50, 71, 177, 269, 353, 362
HpyF3I CTNAG 1 cut(s) 495
HpySE526I ACGT 1 cut(s) 222
Hsp92II CATG 7 cut(s) 233, 237, 283, 331, 361, 380, 472
HspAI GCGC 2 cut(s) 14, 72
Kzo9I GATC 4 cut(s) 97, 238, 284, 307
LguI GCTCTTC 1 cut(s) 85
LmnI GCTCC 1 cut(s) 168
Lsp1109I GCAGC 3 cut(s) 49, 119, 250
MaeI CTAG 1 cut(s) 288
MaeII ACGT 1 cut(s) 222
MalI GATC 4 cut(s) 99, 240, 286, 309
MboI GATC 4 cut(s) 97, 238, 284, 307
MboII GAAGA 1 cut(s) 102
MflI RGATCY 2 cut(s) 238, 284
MhlI GDGCHC 3 cut(s) 173, 367, 496
MlsI TGGCCA 1 cut(s) 356
MluNI TGGCCA 1 cut(s) 356
MlyI GAGTC 1 cut(s) 434
MmeI TCCRAC 2 cut(s) 174, 431
MnlI CCTC 4 cut(s) 159, 354, 364, 443
Mox20I TGGCCA 1 cut(s) 356
Mph1103I ATGCAT 1 cut(s) 235
MroXI GAANNNNTTC 1 cut(s) 147
MscI TGGCCA 1 cut(s) 356
Msp20I TGGCCA 1 cut(s) 356
MspI CCGG 1 cut(s) 31
MvnI CGCG 2 cut(s) 16, 369
MwoI GCNNNNNNNGC 7 cut(s) 22, 50, 71, 177, 269, 353, 362
NdeI CATATG 1 cut(s) 349
NdeII GATC 4 cut(s) 97, 238, 284, 307
NlaIII CATG 7 cut(s) 233, 237, 283, 331, 361, 380, 472
NlaIV GGNNCC 2 cut(s) 84, 170
NruI TCGCGA 1 cut(s) 369
NsiI ATGCAT 1 cut(s) 235
PciSI GCTCTTC 1 cut(s) 85
PdmI GAANNNNTTC 1 cut(s) 147
PinAI ACCGGT 1 cut(s) 30
PkrI GCNGC 4 cut(s) 39, 46, 109, 265
PleI GAGTC 1 cut(s) 433
PpsI GAGTC 1 cut(s) 433
PspN4I GGNNCC 2 cut(s) 84, 170
PspPI GGNCC 3 cut(s) 82, 183, 430
PsuI RGATCY 2 cut(s) 238, 284
RruI TCGCGA 1 cut(s) 369
RsaI GTAC 1 cut(s) 461
RsaNI GTAC 1 cut(s) 460
SapI GCTCTTC 1 cut(s) 85
SatI GCNGC 4 cut(s) 38, 45, 108, 264
Sau3AI GATC 4 cut(s) 97, 238, 284, 307
Sau96I GGNCC 3 cut(s) 82, 183, 430
SchI GAGTC 1 cut(s) 434
SduI GDGCHC 3 cut(s) 173, 367, 496
SetI ASST 8 cut(s) 97, 112, 136, 225, 345, 375, 441, 465
SinI GGWCC 1 cut(s) 82
SmlI CTYRAG 1 cut(s) 434
SmoI CTYRAG 1 cut(s) 434
SsiI CCGC 3 cut(s) 44, 53, 322
SspMI CTAG 1 cut(s) 288
TaaI ACNGT 1 cut(s) 192
TaiI ACGT 1 cut(s) 225
TaqI TCGA 1 cut(s) 310
TauI GCSGC 1 cut(s) 47
TscAI CASTG 1 cut(s) 324
TseI GCWGC 3 cut(s) 37, 107, 263
TspDTI ATGAA 2 cut(s) 268, 316
TspGWI ACGGA 1 cut(s) 472
TspRI CASTG 1 cut(s) 324
VpaK11BI GGWCC 1 cut(s) 82
XmnI GAANNNNTTC 1 cut(s) 147
XspI CTAG 1 cut(s) 288
Zsp2I ATGCAT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.