Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0007297

Physical Location & Seq

Genomic Coordinates Forward (+)

5265931 .. 5271723

Gene Structure 5793 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1074600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1839 bp

ATGGCGGGGAGGACGGACTCGGAGTCCATCCGGGTGCCGTACAGGAACCTGAGGAGGGAGGCGGAAGTGGAAATGATGGTGACGGACGAGCCTCATCATCGTATCGACCTCAATTCGTCTACGTCTTCCTCCCCTAGGGTTCCAAATGGGGGCGGCGGCGGCGGCCTCTCGCCGTCCGGTCAGCCCGGACACAAACACAATACTCTTTTGACGCTTATTCTGAGCTGCACGGTCGCCGCCGGCGTACAGTTCGGTTGGGCGTTGCAGCTTTCGCTTTTAACTCCCTATATACAGACGCTCGGAATAGGGCATGCATTTTCTTCATTTATTTGGCTTTGCGGCCCTATTACAGGCCTTGTGGTTCAACCTTGTGTTGGTATTTGGAGTGACAAATGCTCTTTAAAATTGGGAAGAAGGCGCCCCTTCATTCTTGCAGGATCTCTCATGATTTCAGTTTCCGTGGTGTTAATCGGGTTTTCTGCAGACGTTGGATATTTATTAGGAGATACAAAGGAGCATTGCAGCACATTCAAAGGTACCCGGACAAGGGCAGCTTTTGCCTTTATTATTGGATTTTGGTTGCTGGATCTTGCCAACAATACAGTGCAGGGACCAGCTCGTGCTCTTCTGGCTGATCTAGCAGGCCCCGAGCAACGCAATACTGCAAATGCTGTGTTTTGCTCATGGATGGCCTTTGGTAACATCCTAGGATTTTCTGCTGGTGCAAGTGGAAATTGGCACAGATGGTTTCCTTTCTTGTTGAGTAGAGCTTGCTGTGAAGCCTGTGGAAATCTTAAAGCAGCATTTCTTATTGCAGTGATCTTTCTCACTTTGTGTACACTTGTTACCTTATATTTTGCTGAGGAGGTTCCACTCACTATGTATCAGCAGAATCCTTTATCAGATTCTGCTCCTTTATTGGATGATCCCCAACTAAATCCCCAACAAAGTCGTCTTGAGCTTTCGAAGTTAAAGCGTGATAAGCAAGTTATTGATAATGCAAATGGAAGCAGAACTGACTATGAAAGGGATAAGAATCTAAAGGAGGCCATCTCAAAAGTTGAGGAAGATAAGAATGGTGATTTTAATGATGGACCTGGAGCAGTGTTAGTCAATCTATTGACCAGTTTAAGGCATTTACCACCAGCGATGCATTCAGTGCTAATTGTTATGGCTCTTACATGGTTATCCTGGTTTCCATTCTTTCTTTTTGATACGGATTGGATGGGTAGGGAAGTATATCATGGGGATCCGAATGGGAACTCAGCCAAAGGACAAGCTTATGATCAGGGTGTCAGAGAAGGTGCATTCGGTCTATTATTGAATTCAGTTGTTCTTGGCATCAGTTCTTTCCTAATTGAGCCAATGTGTCAGCAGATGGGTGCAAGACTAGTGTGGGCAATGAGTAATTTTATTGTGTTTGCCTGCATGGCAGGCACCGCTATCATAAGTTTGATATCCGTTGGAGAGTATTCTAAAGGGATTGAACATGTAATTGGTGGGAATCAAAATATCAGGATTGCTTCCCTGATTGTTTTTGCTCTTCTTGGCTTCCCTCTTGCTATTACCTACAGCGTTCCCTTTTCTGTTACAGCAGAGTTGACTGCTGACGCAGGTGGTGGCCAAGGATTGGCTATTGGAGTTTTGAATCTTGCAATTGTTGTTCCACAGATGATTGTGTCTCTTGGTGCGGGGCCATGGGATGCTTTATTTGGGGGAGGAAATATACCTGCCTTTGTTTTGGCCTCCTTCGCTGCCCTTGCTGGTGGCATCATCGCAGCTCGCAGACTACCAAATCTATCGAGTTCTTTCAATTCAACAGGTTTTCATTTTGGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0006810 GO:0006950 GO:0008150 GO:0008515 GO:0008643 GO:0009611 GO:0012505 GO:0015144 GO:0015154 GO:0015157 GO:0015766 GO:0015770 GO:0015772 GO:0022857 GO:0034219 GO:0042995 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0090406 GO:0120025

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MFS_1 MFS_2

Protein Analysis

613

Amino Acids

65.69

Weight (kDa)

6.46

Isoelectric Point (pI)

40.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MFS_1	PF07690	78 - 553	8.2e-18	Major Facilitator Superfamily
MFS_2	PF13347	85 - 292	5.2e-09	MFS/sugar transport protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007297)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G02860 AT2G02860
fragaria_vesca	FvH4_4g31620 FvH4_4g31620 FvH4_4g31620 FvH4_4g31620 FvH4_4g31620
malus_domestica	MD13G1074600.v1.1 MD16G1075900.v1.1
prunus_persica	Prupe.1G271500_v2.0.a1 Prupe.1G271500_v2.0.a1
pyrus_communis	pycom13g06650
rosa_chinensis	RchiOBHm_Chr4g0440131
rosa_laevigata	RLG00000006203
rosa_multiflora	Rmu_sc0001685.1_g000018
rosa_roxburghii	Rroxscaffold_5G00381060
rosa_rugosa	Rorug04G0324400 Rorug04G0324400 Rorug04G0324500 Rorug04G0324500 Rorug04G0324500 Rorug04G0324500
rosa_samantha	Rh4AG375400 Rh4DG381900
rosa_wichuraiana	Rw4G032120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1604
Acc36I	ACCTGC	2 cut(s)	1604, 1738
Acc65I	GGTACC	1 cut(s)	536
AccB1I	GGYRCC	4 cut(s)	34, 417, 536, 1436
AccB7I	CCANNNNNTGG	1 cut(s)	1630
AccI	GTMKAC	1 cut(s)	119
AclWI	GGATC	5 cut(s)	445, 594, 920, 1244, 1257
AcoI	YGGCCR	1 cut(s)	1621
AcsI	RAATTY	1 cut(s)	1324
AcyI	GRCGYC	1 cut(s)	418
AdeI	CACNNNGTG	1 cut(s)	834
AfaI	GTAC	4 cut(s)	41, 246, 538, 838
AfiI	CCNNNNNNNGG	9 cut(s)	55, 135, 149, 350, 374, 546, 547, 1131, 1630
AflIII	ACRYGT	1 cut(s)	1489
AgsI	TTSAA	7 cut(s)	365, 532, 1324, 1487, 1648, 1813, 1818
AhdI	GACNNNNNGTC	1 cut(s)	22
AhlI	ACTAGT	1 cut(s)	1390
AjnI	CCWGG	2 cut(s)	1096, 1190
AjuI	GAANNNNNNNTTGG	2 cut(s)	1479, 1511
AluBI	AGCT	8 cut(s)	225, 268, 554, 617, 770, 961, 1280, 1781
AluI	AGCT	8 cut(s)	225, 268, 554, 617, 770, 961, 1280, 1781
Alw21I	GWGCWC	1 cut(s)	625
Alw26I	GTCTC	1 cut(s)	1686
AlwI	GGATC	5 cut(s)	445, 594, 920, 1244, 1257
AlwNI	CAGNNNCTG	1 cut(s)	908
Ama87I	CYCGRG	1 cut(s)	647
AoxI	GGCC	9 cut(s)	163, 340, 352, 643, 690, 1047, 1621, 1694, 1743
ApeKI	GCWGC	7 cut(s)	225, 265, 522, 551, 800, 1754, 1778
ApoI	RAATTY	1 cut(s)	1324
Asp718I	GGTACC	1 cut(s)	536
AspA2I	CCTAGG	2 cut(s)	134, 706
AspLEI	GCGC	1 cut(s)	420
AspS9I	GGNCC	5 cut(s)	341, 611, 644, 1094, 1694
AsuC2I	CCSGG	3 cut(s)	32, 186, 541
AsuHPI	GGTGA	2 cut(s)	91, 1091
AsuII	TTCGAA	1 cut(s)	965
AvaI	CYCGRG	1 cut(s)	647
AvaII	GGWCC	2 cut(s)	611, 1094
AvrII	CCTAGG	2 cut(s)	134, 706
AxyI	CCTNAGG	1 cut(s)	50
BalI	TGGCCA	1 cut(s)	1623
BamHI	GGATCC	1 cut(s)	1249
BanI	GGYRCC	4 cut(s)	34, 417, 536, 1436
BauI	CACGAG	1 cut(s)	618
BbsI	GAAGAC	1 cut(s)	117
Bbv12I	GWGCWC	1 cut(s)	625
BbvCI	CCTCAGC	1 cut(s)	861
BbvI	GCAGC	7 cut(s)	212, 277, 534, 563, 812, 1741, 1790
BccI	CCATC	8 cut(s)	35, 70, 682, 738, 1058, 1085, 1219, 1372
BceAI	ACGGC	2 cut(s)	22, 157
BciT130I	CCWGG	2 cut(s)	1098, 1192
BclI	TGATCA	1 cut(s)	1285
BcnI	CCSGG	3 cut(s)	32, 186, 541
BcoDI	GTCTC	1 cut(s)	1686
BcuI	ACTAGT	1 cut(s)	1390
BfaI	CTAG	4 cut(s)	135, 638, 707, 1391
BfmI	CTRYAG	2 cut(s)	480, 1570
BfoI	RGCGCY	1 cut(s)	421
BfuAI	ACCTGC	2 cut(s)	1604, 1738
BglI	GCCNNNNNGGC	1 cut(s)	1430
BlnI	CCTAGG	2 cut(s)	134, 706
Bme1390I	CCNGG	5 cut(s)	32, 186, 541, 1098, 1192
Bme18I	GGWCC	2 cut(s)	611, 1094
BmeRI	GACNNNNNGTC	1 cut(s)	22
BmeT110I	CYCGRG	1 cut(s)	647
BmgT120I	GGNCC	5 cut(s)	341, 611, 644, 1094, 1694
BmrFI	CCNGG	5 cut(s)	32, 186, 541, 1098, 1192
BmsI	GCATC	4 cut(s)	1140, 1350, 1693, 1779
BpiI	GAAGAC	1 cut(s)	117
BplI	GAGNNNNNCTC	2 cut(s)	1037, 1069
BpmI	CTGGAG	1 cut(s)	1119
Bpu10I	CCTNAGC	1 cut(s)	861
Bpu14I	TTCGAA	1 cut(s)	965
BpuEI	CTTGAG	1 cut(s)	977
BpuMI	CCSGG	3 cut(s)	32, 186, 541
BsaBI	GATNNNNATC	1 cut(s)	1035
BsaHI	GRCGYC	1 cut(s)	418
BsaJI	CCNNGG	5 cut(s)	134, 459, 706, 1624, 1697
BsaWI	WCCGGW	1 cut(s)	176
Bsc4I	CCNNNNNNNGG	9 cut(s)	55, 135, 149, 350, 374, 546, 547, 1131, 1630
Bse118I	RCCGGY	1 cut(s)	239
Bse1I	ACTGG	1 cut(s)	1125
Bse21I	CCTNAGG	1 cut(s)	50
Bse3DI	GCAATG	2 cut(s)	517, 1407
Bse8I	GATNNNNATC	1 cut(s)	1035
BseBI	CCWGG	2 cut(s)	1098, 1192
BseDI	CCNNGG	5 cut(s)	134, 459, 706, 1624, 1697
BseGI	GGATG	6 cut(s)	27, 693, 702, 928, 1230, 1708
BseJI	GATNNNNATC	1 cut(s)	1035
BseLI	CCNNNNNNNGG	9 cut(s)	55, 135, 149, 350, 374, 546, 547, 1131, 1630
BseMI	GCAATG	2 cut(s)	517, 1407
BseMII	CTCAG	4 cut(s)	41, 212, 852, 1278
BseNI	ACTGG	1 cut(s)	1125
BseRI	GAGGAG	2 cut(s)	67, 878
BseXI	GCAGC	7 cut(s)	212, 277, 534, 563, 812, 1741, 1790
BsgI	GTGCAG	2 cut(s)	211, 626
Bsh1285I	CGRYCG	1 cut(s)	234
BshFI	GGCC	9 cut(s)	165, 342, 354, 645, 692, 1049, 1623, 1696, 1745
BshNI	GGYRCC	4 cut(s)	34, 417, 536, 1436
BsiEI	CGRYCG	1 cut(s)	234
BsiHKAI	GWGCWC	1 cut(s)	625
BsiHKCI	CYCGRG	1 cut(s)	647
BsiSI	CCGG	5 cut(s)	31, 177, 186, 240, 541
BslFI	GGGAC	1 cut(s)	624
BslI	CCNNNNNNNGG	9 cut(s)	55, 135, 149, 350, 374, 546, 547, 1131, 1630
BsmAI	GTCTC	1 cut(s)	1686
BsmFI	GGGAC	1 cut(s)	624
BsmI	GAATGC	2 cut(s)	1153, 1307
BsnI	GGCC	9 cut(s)	165, 342, 354, 645, 692, 1049, 1623, 1696, 1745
BsoBI	CYCGRG	1 cut(s)	647
Bsp119I	TTCGAA	1 cut(s)	965
Bsp1286I	GDGCHC	1 cut(s)	625
Bsp1407I	TGTACA	1 cut(s)	836
Bsp143I	GATC	7 cut(s)	437, 586, 634, 819, 925, 1249, 1285
Bsp19I	CCATGG	1 cut(s)	1697
BspANI	GGCC	9 cut(s)	165, 342, 354, 645, 692, 1049, 1623, 1696, 1745
BspCNI	CTCAG	4 cut(s)	42, 213, 853, 1277
BspHI	TCATGA	1 cut(s)	444
BspMAI	CTGCAG	1 cut(s)	484
BspMI	ACCTGC	2 cut(s)	1604, 1738
BspPI	GGATC	5 cut(s)	445, 594, 920, 1244, 1257
BspQI	GCTCTTC	2 cut(s)	630, 1548
BspT104I	TTCGAA	1 cut(s)	965
BspT107I	GGYRCC	4 cut(s)	34, 417, 536, 1436
BsrDI	GCAATG	2 cut(s)	517, 1407
BsrFI	RCCGGY	1 cut(s)	239
BsrGI	TGTACA	1 cut(s)	836
BsrI	ACTGG	1 cut(s)	1125
BssAI	RCCGGY	1 cut(s)	239
BssECI	CCNNGG	5 cut(s)	134, 459, 706, 1624, 1697
BssMI	GATC	7 cut(s)	437, 586, 634, 819, 925, 1249, 1285
BssNI	GRCGYC	1 cut(s)	418
BssSI	CACGAG	1 cut(s)	618
BssT1I	CCWWGG	4 cut(s)	134, 706, 1624, 1697
Bst2BI	CACGAG	1 cut(s)	618
Bst2UI	CCWGG	2 cut(s)	1098, 1192
Bst4CI	ACNGT	3 cut(s)	232, 249, 604
Bst6I	CTCTTC	2 cut(s)	630, 1548
BstACI	GRCGYC	1 cut(s)	418
BstAPI	GCANNNNNTGC	2 cut(s)	557, 1159
BstAUI	TGTACA	1 cut(s)	836
BstBI	TTCGAA	1 cut(s)	965
BstC8I	GCNNGC	7 cut(s)	241, 312, 643, 772, 1426, 1435, 1783
BstDEI	CTNAG	4 cut(s)	50, 221, 861, 1264
BstDSI	CCRYGG	2 cut(s)	459, 1697
BstENI	CCTNNNNNAGG	2 cut(s)	133, 348
BstF5I	GGATG	6 cut(s)	27, 693, 702, 928, 1230, 1708
BstH2I	RGCGCY	1 cut(s)	421
BstHHI	GCGC	1 cut(s)	420
BstKTI	GATC	7 cut(s)	440, 589, 637, 822, 928, 1252, 1288
BstMAI	GTCTC	1 cut(s)	1686
BstMBI	GATC	7 cut(s)	437, 586, 634, 819, 925, 1249, 1285
BstMCI	CGRYCG	1 cut(s)	234
BstNI	CCWGG	2 cut(s)	1098, 1192
BstNSI	RCATGY	2 cut(s)	314, 1493
BstSCI	CCNGG	5 cut(s)	30, 184, 539, 1096, 1190
BstSFI	CTRYAG	2 cut(s)	480, 1570
BstV1I	GCAGC	7 cut(s)	212, 277, 534, 563, 812, 1741, 1790
BstV2I	GAAGAC	1 cut(s)	117
BstX2I	RGATCY	3 cut(s)	437, 586, 1249
BstYI	RGATCY	3 cut(s)	437, 586, 1249
Bsu36I	CCTNAGG	1 cut(s)	50
BsuRI	GGCC	9 cut(s)	165, 342, 354, 645, 692, 1049, 1623, 1696, 1745
BtgI	CCRYGG	2 cut(s)	459, 1697
BtgZI	GCGATG	2 cut(s)	1163, 1759
BtsCI	GGATG	6 cut(s)	27, 693, 702, 928, 1230, 1708
BtsI	GCAGTG	2 cut(s)	822, 1110
BtsIMutI	CAGTG	4 cut(s)	609, 822, 1110, 1164
BveI	ACCTGC	2 cut(s)	1604, 1738
Cac8I	GCNNGC	7 cut(s)	241, 312, 643, 772, 1426, 1435, 1783
CaiI	CAGNNNCTG	1 cut(s)	908
CciI	TCATGA	1 cut(s)	444
CfoI	GCGC	1 cut(s)	420
Cfr10I	RCCGGY	1 cut(s)	239
Cfr13I	GGNCC	5 cut(s)	341, 611, 644, 1094, 1694
CseI	GACGC	3 cut(s)	220, 304, 1619
Csp6I	GTAC	4 cut(s)	40, 245, 537, 837
CviAII	CATG	8 cut(s)	311, 445, 684, 1182, 1244, 1429, 1490, 1698
CviQI	GTAC	4 cut(s)	40, 245, 537, 837
DdeI	CTNAG	4 cut(s)	50, 221, 861, 1264
DinI	GGCGCC	1 cut(s)	419
DpnI	GATC	7 cut(s)	439, 588, 636, 821, 927, 1251, 1287
DpnII	GATC	7 cut(s)	437, 586, 634, 819, 925, 1249, 1285
DraI	TTTAAA	1 cut(s)	402
DraIII	CACNNNGTG	1 cut(s)	834
DriI	GACNNNNNGTC	1 cut(s)	22
EaeI	YGGCCR	1 cut(s)	1621
Eam1104I	CTCTTC	2 cut(s)	630, 1548
Eam1105I	GACNNNNNGTC	1 cut(s)	22
EarI	CTCTTC	2 cut(s)	630, 1548
EciI	GGCGGA	1 cut(s)	77
Eco130I	CCWWGG	4 cut(s)	134, 706, 1624, 1697
Eco147I	AGGCCT	1 cut(s)	354
Eco32I	GATATC	1 cut(s)	1458
Eco47I	GGWCC	2 cut(s)	611, 1094
Eco81I	CCTNAGG	1 cut(s)	50
Eco88I	CYCGRG	1 cut(s)	647
EcoNI	CCTNNNNNAGG	2 cut(s)	133, 348
EcoO109I	RGGNCCY	1 cut(s)	644
EcoRI	GAATTC	1 cut(s)	1324
EcoRII	CCWGG	2 cut(s)	1096, 1190
EcoRV	GATATC	1 cut(s)	1458
EcoT14I	CCWWGG	4 cut(s)	134, 706, 1624, 1697
EcoT22I	ATGCAT	2 cut(s)	316, 1155
EgeI	GGCGCC	1 cut(s)	419
EheI	GGCGCC	1 cut(s)	419
ErhI	CCWWGG	4 cut(s)	134, 706, 1624, 1697
FaeI	CATG	8 cut(s)	314, 448, 687, 1185, 1247, 1432, 1493, 1701
FalI	AAGNNNNNCTT	2 cut(s)	538, 570
FaqI	GGGAC	1 cut(s)	624
FatI	CATG	8 cut(s)	310, 444, 683, 1181, 1243, 1428, 1489, 1697
FauI	CCCGC	1 cut(s)	1684
FbaI	TGATCA	1 cut(s)	1285
FblI	GTMKAC	1 cut(s)	119
FokI	GGATG	6 cut(s)	14, 689, 700, 935, 1237, 1715
FspBI	CTAG	4 cut(s)	135, 638, 707, 1391
GlaI	GCGC	1 cut(s)	419
GsuI	CTGGAG	1 cut(s)	1119
HaeII	RGCGCY	1 cut(s)	421
HaeIII	GGCC	9 cut(s)	165, 342, 354, 645, 692, 1049, 1623, 1696, 1745
HapII	CCGG	5 cut(s)	31, 177, 186, 240, 541
HgaI	GACGC	3 cut(s)	220, 304, 1619
HhaI	GCGC	1 cut(s)	420
Hin1I	GRCGYC	1 cut(s)	418
Hin1II	CATG	8 cut(s)	314, 448, 687, 1185, 1247, 1432, 1493, 1701
Hin6I	GCGC	1 cut(s)	418
HinP1I	GCGC	1 cut(s)	418
HincII	GTYRAC	1 cut(s)	1602
HindII	GTYRAC	1 cut(s)	1602
HindIII	AAGCTT	1 cut(s)	1278
HinfI	GANTC	7 cut(s)	17, 23, 892, 905, 1036, 1504, 1648
HpaII	CCGG	5 cut(s)	31, 177, 186, 240, 541
HphI	GGTGA	2 cut(s)	91, 1091
Hpy166II	GTNNAC	4 cut(s)	120, 837, 839, 1602
Hpy188I	TCNGA	6 cut(s)	22, 222, 302, 904, 1254, 1298
Hpy188III	TCNNGA	3 cut(s)	445, 956, 1516
Hpy8I	GTNNAC	4 cut(s)	120, 837, 839, 1602
HpyAV	CCTTC	4 cut(s)	408, 433, 1295, 1759
HpyCH4III	ACNGT	3 cut(s)	232, 249, 604
HpyCH4IV	ACGT	2 cut(s)	122, 486
HpyF3I	CTNAG	4 cut(s)	50, 221, 861, 1264
HpySE526I	ACGT	2 cut(s)	122, 486
Hsp92I	GRCGYC	1 cut(s)	418
Hsp92II	CATG	8 cut(s)	314, 448, 687, 1185, 1247, 1432, 1493, 1701
HspAI	GCGC	1 cut(s)	418
KasI	GGCGCC	1 cut(s)	417
KpnI	GGTACC	1 cut(s)	540
KroI	GCCGGC	1 cut(s)	239
KroNI	GCCGGC	1 cut(s)	241
Ksp22I	TGATCA	1 cut(s)	1285
Kzo9I	GATC	7 cut(s)	437, 586, 634, 819, 925, 1249, 1285
LguI	GCTCTTC	2 cut(s)	630, 1548
LmnI	GCTCC	3 cut(s)	514, 916, 1100
Lsp1109I	GCAGC	7 cut(s)	212, 277, 534, 563, 812, 1741, 1790
LweI	GCATC	4 cut(s)	1140, 1350, 1693, 1779
MaeI	CTAG	4 cut(s)	135, 638, 707, 1391
MaeII	ACGT	2 cut(s)	122, 486
MaeIII	GTNAC	5 cut(s)	79, 386, 698, 844, 1588
MalI	GATC	7 cut(s)	439, 588, 636, 821, 927, 1251, 1287
MboI	GATC	7 cut(s)	437, 586, 634, 819, 925, 1249, 1285
MboII	GAAGA	6 cut(s)	117, 312, 423, 617, 1079, 1535
MfeI	CAATTG	1 cut(s)	1656
MflI	RGATCY	3 cut(s)	437, 586, 1249
MhlI	GDGCHC	1 cut(s)	625
MlsI	TGGCCA	1 cut(s)	1623
MluNI	TGGCCA	1 cut(s)	1623
Mly113I	GGCGCC	1 cut(s)	418
MlyI	GAGTC	2 cut(s)	11, 32
MmeI	TCCRAC	2 cut(s)	469, 1444
Mox20I	TGGCCA	1 cut(s)	1623
Mph1103I	ATGCAT	2 cut(s)	316, 1155
MreI	CGCCGGCG	1 cut(s)	239
MroNI	GCCGGC	1 cut(s)	239
MscI	TGGCCA	1 cut(s)	1623
MseI	TTAA	7 cut(s)	278, 401, 467, 795, 971, 1086, 1130
MslI	CAYNNNNRTG	1 cut(s)	32
Msp20I	TGGCCA	1 cut(s)	1623
MspI	CCGG	5 cut(s)	31, 177, 186, 240, 541
MspR9I	CCNGG	5 cut(s)	32, 186, 541, 1098, 1192
MunI	CAATTG	1 cut(s)	1656
Mva1269I	GAATGC	2 cut(s)	1153, 1307
MvaI	CCWGG	2 cut(s)	1098, 1192
NaeI	GCCGGC	1 cut(s)	241
NarI	GGCGCC	1 cut(s)	418
NciI	CCSGG	3 cut(s)	32, 186, 541
NcoI	CCATGG	1 cut(s)	1697
NdeII	GATC	7 cut(s)	437, 586, 634, 819, 925, 1249, 1285
NgoMIV	GCCGGC	1 cut(s)	239
NlaIII	CATG	8 cut(s)	314, 448, 687, 1185, 1247, 1432, 1493, 1701
NmuCI	GTSAC	2 cut(s)	79, 386
NsiI	ATGCAT	2 cut(s)	316, 1155
NspI	RCATGY	2 cut(s)	314, 1493
NspV	TTCGAA	1 cut(s)	965
PaeI	GCATGC	1 cut(s)	314
PagI	TCATGA	1 cut(s)	444
PaqCI	CACCTGC	1 cut(s)	1604
PceI	AGGCCT	1 cut(s)	354
PciI	ACATGT	1 cut(s)	1489
PciSI	GCTCTTC	2 cut(s)	630, 1548
PcsI	WCGNNNNNNNCGW	1 cut(s)	240
PctI	GAATGC	2 cut(s)	1153, 1307
PdiI	GCCGGC	1 cut(s)	241
PfeI	GAWTC	5 cut(s)	892, 905, 1036, 1504, 1648
PflMI	CCANNNNNTGG	1 cut(s)	1630
PleI	GAGTC	2 cut(s)	11, 31
PluTI	GGCGCC	1 cut(s)	421
PpsI	GAGTC	2 cut(s)	11, 31
PscI	ACATGT	1 cut(s)	1489
Psp6I	CCWGG	2 cut(s)	1096, 1190
PspGI	CCWGG	2 cut(s)	1096, 1190
PspPI	GGNCC	5 cut(s)	341, 611, 644, 1094, 1694
PstI	CTGCAG	1 cut(s)	484
PstNI	CAGNNNCTG	1 cut(s)	908
PsuI	RGATCY	3 cut(s)	437, 586, 1249
RsaI	GTAC	4 cut(s)	41, 246, 538, 838
RsaNI	GTAC	4 cut(s)	40, 245, 537, 837
RseI	CAYNNNNRTG	1 cut(s)	32
SapI	GCTCTTC	2 cut(s)	630, 1548
SaqAI	TTAA	7 cut(s)	278, 401, 467, 795, 971, 1086, 1130
Sau3AI	GATC	7 cut(s)	437, 586, 634, 819, 925, 1249, 1285
Sau96I	GGNCC	5 cut(s)	341, 611, 644, 1094, 1694
SchI	GAGTC	2 cut(s)	11, 32
ScrFI	CCNGG	5 cut(s)	32, 186, 541, 1098, 1192
SduI	GDGCHC	1 cut(s)	625
SfaNI	GCATC	4 cut(s)	1140, 1350, 1693, 1779
SfcI	CTRYAG	2 cut(s)	480, 1570
SfoI	GGCGCC	1 cut(s)	419
SfuI	TTCGAA	1 cut(s)	965
SgrAI	CRCCGGYG	1 cut(s)	239
SinI	GGWCC	2 cut(s)	611, 1094
SmiMI	CAYNNNNRTG	1 cut(s)	32
SmlI	CTYRAG	1 cut(s)	956
SmoI	CTYRAG	1 cut(s)	956
SpeI	ACTAGT	1 cut(s)	1390
SphI	GCATGC	1 cut(s)	314
SseBI	AGGCCT	1 cut(s)	354
SspDI	GGCGCC	1 cut(s)	417
SspMI	CTAG	4 cut(s)	135, 638, 707, 1391
StuI	AGGCCT	1 cut(s)	354
StyD4I	CCNGG	5 cut(s)	30, 184, 539, 1096, 1190
StyI	CCWWGG	4 cut(s)	134, 706, 1624, 1697
TaaI	ACNGT	3 cut(s)	232, 249, 604
TaiI	ACGT	2 cut(s)	125, 489
TaqI	TCGA	3 cut(s)	105, 965, 1802
TaqII	GACCGA	1 cut(s)	1301
TatI	WGTACW	1 cut(s)	836
TauI	GCSGC	6 cut(s)	156, 159, 162, 165, 239, 342
TfiI	GAWTC	5 cut(s)	892, 905, 1036, 1504, 1648
Tru1I	TTAA	7 cut(s)	278, 401, 467, 795, 971, 1086, 1130
Tru9I	TTAA	7 cut(s)	278, 401, 467, 795, 971, 1086, 1130
TscAI	CASTG	4 cut(s)	609, 822, 1110, 1164
TseFI	GTSAC	2 cut(s)	79, 386
TseI	GCWGC	7 cut(s)	225, 265, 522, 551, 800, 1754, 1778
Tsp45I	GTSAC	2 cut(s)	79, 386
TspDTI	ATGAA	4 cut(s)	312, 415, 1038, 1817
TspGWI	ACGGA	5 cut(s)	29, 98, 448, 1232, 1450
TspRI	CASTG	4 cut(s)	609, 822, 1110, 1164
Van91I	CCANNNNNTGG	1 cut(s)	1630
VpaK11BI	GGWCC	2 cut(s)	611, 1094
XagI	CCTNNNNNAGG	2 cut(s)	133, 348
XapI	RAATTY	1 cut(s)	1324
XceI	RCATGY	2 cut(s)	314, 1493
XmaJI	CCTAGG	2 cut(s)	134, 706
XmiI	GTMKAC	1 cut(s)	119
XspI	CTAG	4 cut(s)	135, 638, 707, 1391
Zsp2I	ATGCAT	2 cut(s)	316, 1155

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.