Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0007297

Physical Location & Seq

Genomic Coordinates Forward (+)

5310417 .. 5316206

Gene Structure 5790 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1075900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1824 bp

ATGGCGGGGAGGACGGACTCGGAGTCCATCCGGGTGCCGTACAGGAACCTGAGGAGGGATGCGGAAGTGGAAATGATGGTGACGGACGAGCCTCATCATCGTATCGACCTCAATTCGCCTACGTCTTCCTCCCCTAGGGTTCCAAATGGGAGCGGCGGCGGCCTCTCGCCGCCGGGACAGCCCGGACACAAACACCATACTCTTTTGACGCTCATTCTCAGCTGCACGGTCGCCGCCGGTGTGCAGTTCGGTTGGGCGTTGCAGCTTTCGCTTTTAACTCCTTATATACAGACTCTCGGAATAGGGCATGCCTTTTCTTCATTTATTTGGCTTTGTGGCCCTATTACAGGCCTTGTGGTTCAACCTTGTGTTGGTATTTGGAGTGACAAATGCTCTTTAAAATTGGGAAGAAGACGCCCCTTCATTCTTGCAGGATCTCTCATGATTTCTGTTTCCGTGGTGTTAATCGGGTTTTCTGCAGACGTTGGATACTTATTAGGAGATACAAAGGAGCATTGCAGCACATTCAAAGGTACCCGGACAAGGGCAGCCTTTGTCTTTATTATTGGATTTTGGTTGCTGGATCTTGCCAACAATACAGTGCAGGGACCAGCTCGTGCTCTTCTGGCTGATCTAGCAGGCCCCGAGCAACGTAATACTGCAAATGCCGTGTTTTGCTCATGGATGGCCGTTGGTAACATACTAGGATTTTCTGCTGGTGCAAGTGGAAATTGGCACAGATGGTTTCCTTTCCTTTTGAGTAGAGCTTGCTGTGAAGCCTGTGGAAATCTTAAAGCAGCATTTCTTATTGCGGTGATCTTTCTCACTTTGTGTACACTGGTTACAATGTATTTTGCTGAGGAGGTTCCGCTCACTGCGTATCAGCCGAACCCTTTATCAGATTCTGCTCCTTTATTGGATGATCCCCAACAACATGGTCTTGAGCTTTCTAAGTTGAAGCGTGATAAGCAAGTTATTGATAATGCAAATGGAAGCAGAACTGACTATGAAAGGGATATAAATCTGAAGGAGGCCATCTCAAAAGTTGAGGAAGATAAGAATGGTGGTTTTAATGACGGACCTGGAGCAGTGTTAGTCAATCTGTTGACCAGTTTAAGGCATTTACCACCAGCAATGCATTCAGTGCTAATTGTTATGGCTCTTACATGGTTATCCTGGTTTCCATTCTTTCTTTTTGATACGGATTGGATGGGTAGGGAAGTATATCATGGGGATCCGAAAGGGAACTCGGCCAAAGTACAAGCTTATGATCAGGGTGTCAGAGAAGGTGCATTCGGTTTATTATTGAATTCAGTTGTTCTTGGCATCAGTTCTTTCCTAATTGAGCCAATGTGCCAGCGGATGGGTGCAAGACTAGTGTGGGCAATGAGCAATTTTATTGTGTTTGCCTGCATGGCAGGCACCGCTATCATAAGTTTAATATCTGTTACAGAGTATTCTAAAGGGATTGAACATGTAATTGGGGGGAATGAAAATATCAGGATTGCTTCCCTGATTGTTTTTGCTCTTCTTGGCTTCCCACTTGCTATTACCTACAGTGTTCCCTTTTCCGTTACAGCAGAGTTGACTGCCGACGCAGGTGGTGGCCAAGGACTGGCTATCGGAGTTTTGAATCTTGCAATTGTTATTCCACAGATGATTGTGTCTCTTGGTGCGGGGCCATGGGATGCTTTATTTGGGGGAGGAAATATACCTGCCTTTGTTTTGGCCTCCTTCGCTGCCCTTGCTGGTGGCATTATCGCGGCTCGCAGACTACCAATTCTTTCGAGTTCCCTCAAGTCAACCGGTTTTCATTTTGGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0006810 GO:0006950 GO:0008150 GO:0008515 GO:0008643 GO:0009611 GO:0012505 GO:0015144 GO:0015154 GO:0015157 GO:0015766 GO:0015770 GO:0015772 GO:0022857 GO:0034219 GO:0042995 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0090406 GO:0120025

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MFS_1 MFS_2

Protein Analysis

608

Amino Acids

65.15

Weight (kDa)

6.7

Isoelectric Point (pI)

39.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MFS_1	PF07690	77 - 549	1.3e-19	Major Facilitator Superfamily
MFS_2	PF13347	84 - 291	1.2e-09	MFS/sugar transport protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007297)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G02860 AT2G02860
fragaria_vesca	FvH4_4g31620 FvH4_4g31620 FvH4_4g31620 FvH4_4g31620 FvH4_4g31620
malus_domestica	MD13G1074600.v1.1 MD16G1075900.v1.1
prunus_persica	Prupe.1G271500_v2.0.a1 Prupe.1G271500_v2.0.a1
pyrus_communis	pycom13g06650
rosa_chinensis	RchiOBHm_Chr4g0440131
rosa_laevigata	RLG00000006203
rosa_multiflora	Rmu_sc0001685.1_g000018
rosa_roxburghii	Rroxscaffold_5G00381060
rosa_rugosa	Rorug04G0324400 Rorug04G0324400 Rorug04G0324500 Rorug04G0324500 Rorug04G0324500 Rorug04G0324500
rosa_samantha	Rh4AG375400 Rh4DG381900
rosa_wichuraiana	Rw4G032120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1589
Acc36I	ACCTGC	2 cut(s)	1589, 1723
Acc65I	GGTACC	1 cut(s)	533
AccB1I	GGYRCC	3 cut(s)	34, 533, 1421
AccB7I	CCANNNNNTGG	2 cut(s)	1363, 1615
AccBSI	CCGCTC	2 cut(s)	153, 871
AccII	CGCG	1 cut(s)	1763
AclWI	GGATC	5 cut(s)	442, 591, 917, 1229, 1242
AcoI	YGGCCR	3 cut(s)	687, 1251, 1606
AcsI	RAATTY	1 cut(s)	1309
AcuI	CTGAAG	1 cut(s)	1046
AcyI	GRCGYC	1 cut(s)	415
AdeI	CACNNNGTG	1 cut(s)	831
AfaI	GTAC	4 cut(s)	41, 535, 835, 1260
AfiI	CCNNNNNNNGG	9 cut(s)	55, 135, 347, 371, 543, 544, 1116, 1363, 1615
AflIII	ACRYGT	1 cut(s)	1474
AgeI	ACCGGT	1 cut(s)	1805
AgsI	TTSAA	6 cut(s)	362, 529, 958, 1309, 1472, 1633
AhdI	GACNNNNNGTC	1 cut(s)	22
AhlI	ACTAGT	1 cut(s)	1375
AjnI	CCWGG	2 cut(s)	1081, 1175
AjuI	GAANNNNNNNTTGG	2 cut(s)	1464, 1496
AluBI	AGCT	6 cut(s)	222, 265, 614, 767, 946, 1265
AluI	AGCT	6 cut(s)	222, 265, 614, 767, 946, 1265
Alw21I	GWGCWC	1 cut(s)	622
Alw26I	GTCTC	1 cut(s)	1671
AlwI	GGATC	5 cut(s)	442, 591, 917, 1229, 1242
AlwNI	CAGNNNCTG	1 cut(s)	905
Ama87I	CYCGRG	1 cut(s)	644
ApeKI	GCWGC	6 cut(s)	222, 262, 519, 548, 797, 1739
ApoI	RAATTY	1 cut(s)	1309
AsiGI	ACCGGT	1 cut(s)	1805
Asp718I	GGTACC	1 cut(s)	533
AspA2I	CCTAGG	1 cut(s)	134
AspS9I	GGNCC	5 cut(s)	338, 608, 641, 1079, 1679
AsuC2I	CCSGG	4 cut(s)	32, 174, 183, 538
AsuHPI	GGTGA	2 cut(s)	91, 826
AvaI	CYCGRG	1 cut(s)	644
AvaII	GGWCC	2 cut(s)	608, 1079
AvrII	CCTAGG	1 cut(s)	134
AxyI	CCTNAGG	1 cut(s)	50
BalI	TGGCCA	1 cut(s)	1608
BamHI	GGATCC	1 cut(s)	1234
BanI	GGYRCC	3 cut(s)	34, 533, 1421
BauI	CACGAG	1 cut(s)	615
BbsI	GAAGAC	2 cut(s)	117, 418
Bbv12I	GWGCWC	1 cut(s)	622
BbvCI	CCTCAGC	1 cut(s)	858
BbvI	GCAGC	6 cut(s)	209, 274, 531, 560, 809, 1726
BccI	CCATC	7 cut(s)	35, 70, 679, 735, 1043, 1204, 1357
BceAI	ACGGC	3 cut(s)	22, 653, 674
BciT130I	CCWGG	2 cut(s)	1083, 1177
BciVI	GTATCC	1 cut(s)	482
BclI	TGATCA	1 cut(s)	1270
BcnI	CCSGG	4 cut(s)	32, 174, 183, 538
BcoDI	GTCTC	1 cut(s)	1671
BcuI	ACTAGT	1 cut(s)	1375
BfaI	CTAG	4 cut(s)	135, 635, 704, 1376
BfmI	CTRYAG	2 cut(s)	477, 1555
BfuAI	ACCTGC	2 cut(s)	1589, 1723
BfuI	GTATCC	1 cut(s)	482
BglI	GCCNNNNNGGC	1 cut(s)	1415
BlnI	CCTAGG	1 cut(s)	134
Bme1390I	CCNGG	6 cut(s)	32, 174, 183, 538, 1083, 1177
Bme18I	GGWCC	2 cut(s)	608, 1079
BmeRI	GACNNNNNGTC	1 cut(s)	22
BmeT110I	CYCGRG	1 cut(s)	644
BmgT120I	GGNCC	5 cut(s)	338, 608, 641, 1079, 1679
BmrFI	CCNGG	6 cut(s)	32, 174, 183, 538, 1083, 1177
BmsI	GCATC	3 cut(s)	49, 1335, 1678
BpiI	GAAGAC	2 cut(s)	117, 418
BplI	GAGNNNNNCTC	2 cut(s)	1022, 1054
BpmI	CTGGAG	1 cut(s)	1104
Bpu10I	CCTNAGC	1 cut(s)	858
BpuEI	CTTGAG	2 cut(s)	962, 1781
BpuMI	CCSGG	4 cut(s)	32, 174, 183, 538
BsaBI	GATNNNNATC	1 cut(s)	1020
BsaHI	GRCGYC	1 cut(s)	415
BsaJI	CCNNGG	4 cut(s)	134, 456, 1609, 1682
BsaWI	WCCGGW	1 cut(s)	1805
Bsc4I	CCNNNNNNNGG	9 cut(s)	55, 135, 347, 371, 543, 544, 1116, 1363, 1615
Bse118I	RCCGGY	2 cut(s)	236, 1805
Bse1I	ACTGG	3 cut(s)	843, 1110, 1620
Bse21I	CCTNAGG	1 cut(s)	50
Bse3DI	GCAATG	3 cut(s)	514, 1140, 1392
Bse8I	GATNNNNATC	1 cut(s)	1020
BseBI	CCWGG	2 cut(s)	1083, 1177
BseDI	CCNNGG	4 cut(s)	134, 456, 1609, 1682
BseGI	GGATG	7 cut(s)	27, 64, 690, 925, 1215, 1368, 1693
BseJI	GATNNNNATC	1 cut(s)	1020
BseLI	CCNNNNNNNGG	9 cut(s)	55, 135, 347, 371, 543, 544, 1116, 1363, 1615
BseMI	GCAATG	3 cut(s)	514, 1140, 1392
BseMII	CTCAG	3 cut(s)	41, 232, 849
BseNI	ACTGG	3 cut(s)	843, 1110, 1620
BseRI	GAGGAG	2 cut(s)	67, 875
BseXI	GCAGC	6 cut(s)	209, 274, 531, 560, 809, 1726
BsgI	GTGCAG	3 cut(s)	208, 263, 623
Bsh1236I	CGCG	1 cut(s)	1763
Bsh1285I	CGRYCG	1 cut(s)	231
BshNI	GGYRCC	3 cut(s)	34, 533, 1421
BshTI	ACCGGT	1 cut(s)	1805
BsiEI	CGRYCG	1 cut(s)	231
BsiHKAI	GWGCWC	1 cut(s)	622
BsiHKCI	CYCGRG	1 cut(s)	644
BsiSI	CCGG	6 cut(s)	31, 173, 183, 237, 538, 1806
BslFI	GGGAC	2 cut(s)	189, 621
BslI	CCNNNNNNNGG	9 cut(s)	55, 135, 347, 371, 543, 544, 1116, 1363, 1615
BsmAI	GTCTC	1 cut(s)	1671
BsmFI	GGGAC	2 cut(s)	189, 621
BsmI	GAATGC	2 cut(s)	1138, 1292
BsoBI	CYCGRG	1 cut(s)	644
Bsp1286I	GDGCHC	1 cut(s)	622
Bsp1407I	TGTACA	1 cut(s)	833
Bsp143I	GATC	7 cut(s)	434, 583, 631, 816, 922, 1234, 1270
Bsp19I	CCATGG	1 cut(s)	1682
BspCNI	CTCAG	3 cut(s)	42, 231, 850
BspFNI	CGCG	1 cut(s)	1763
BspHI	TCATGA	1 cut(s)	441
BspMAI	CTGCAG	1 cut(s)	481
BspMI	ACCTGC	2 cut(s)	1589, 1723
BspPI	GGATC	5 cut(s)	442, 591, 917, 1229, 1242
BspQI	GCTCTTC	2 cut(s)	627, 1533
BspT107I	GGYRCC	3 cut(s)	34, 533, 1421
BsrBI	CCGCTC	2 cut(s)	153, 871
BsrDI	GCAATG	3 cut(s)	514, 1140, 1392
BsrFI	RCCGGY	2 cut(s)	236, 1805
BsrGI	TGTACA	1 cut(s)	833
BsrI	ACTGG	3 cut(s)	843, 1110, 1620
BssAI	RCCGGY	2 cut(s)	236, 1805
BssECI	CCNNGG	4 cut(s)	134, 456, 1609, 1682
BssMI	GATC	7 cut(s)	434, 583, 631, 816, 922, 1234, 1270
BssNI	GRCGYC	1 cut(s)	415
BssSI	CACGAG	1 cut(s)	615
BssT1I	CCWWGG	3 cut(s)	134, 1609, 1682
Bst2BI	CACGAG	1 cut(s)	615
Bst2UI	CCWGG	2 cut(s)	1083, 1177
Bst4CI	ACNGT	3 cut(s)	229, 601, 1559
Bst6I	CTCTTC	2 cut(s)	627, 1533
BstACI	GRCGYC	1 cut(s)	415
BstAPI	GCANNNNNTGC	1 cut(s)	1144
BstAUI	TGTACA	1 cut(s)	833
BstC8I	GCNNGC	7 cut(s)	309, 640, 769, 1358, 1411, 1420, 1768
BstDEI	CTNAG	4 cut(s)	50, 218, 858, 951
BstDSI	CCRYGG	2 cut(s)	456, 1682
BstENI	CCTNNNNNAGG	2 cut(s)	133, 345
BstF5I	GGATG	7 cut(s)	27, 64, 690, 925, 1215, 1368, 1693
BstFNI	CGCG	1 cut(s)	1763
BstKTI	GATC	7 cut(s)	437, 586, 634, 819, 925, 1237, 1273
BstMAI	GTCTC	1 cut(s)	1671
BstMBI	GATC	7 cut(s)	434, 583, 631, 816, 922, 1234, 1270
BstMCI	CGRYCG	1 cut(s)	231
BstNI	CCWGG	2 cut(s)	1083, 1177
BstNSI	RCATGY	2 cut(s)	311, 1478
BstSCI	CCNGG	6 cut(s)	30, 172, 181, 536, 1081, 1175
BstSFI	CTRYAG	2 cut(s)	477, 1555
BstUI	CGCG	1 cut(s)	1763
BstV1I	GCAGC	6 cut(s)	209, 274, 531, 560, 809, 1726
BstV2I	GAAGAC	2 cut(s)	117, 418
BstX2I	RGATCY	3 cut(s)	434, 583, 1234
BstXI	CCANNNNNNTGG	1 cut(s)	935
BstYI	RGATCY	3 cut(s)	434, 583, 1234
Bsu36I	CCTNAGG	1 cut(s)	50
BsuI	GTATCC	1 cut(s)	482
BtgI	CCRYGG	2 cut(s)	456, 1682
BtsCI	GGATG	7 cut(s)	27, 64, 690, 925, 1215, 1368, 1693
BtsI	GCAGTG	2 cut(s)	873, 1095
BtsIMutI	CAGTG	6 cut(s)	606, 836, 873, 1095, 1149, 1564
BveI	ACCTGC	2 cut(s)	1589, 1723
Cac8I	GCNNGC	7 cut(s)	309, 640, 769, 1358, 1411, 1420, 1768
CaiI	CAGNNNCTG	1 cut(s)	905
CciI	TCATGA	1 cut(s)	441
Cfr10I	RCCGGY	2 cut(s)	236, 1805
Cfr13I	GGNCC	5 cut(s)	338, 608, 641, 1079, 1679
CseI	GACGC	3 cut(s)	217, 423, 1604
Csp6I	GTAC	4 cut(s)	40, 534, 834, 1259
CspAI	ACCGGT	1 cut(s)	1805
CviAII	CATG	9 cut(s)	308, 442, 681, 935, 1167, 1229, 1414, 1475, 1683
CviQI	GTAC	4 cut(s)	40, 534, 834, 1259
DdeI	CTNAG	4 cut(s)	50, 218, 858, 951
DpnI	GATC	7 cut(s)	436, 585, 633, 818, 924, 1236, 1272
DpnII	GATC	7 cut(s)	434, 583, 631, 816, 922, 1234, 1270
DraI	TTTAAA	1 cut(s)	399
DraIII	CACNNNGTG	1 cut(s)	831
DriI	GACNNNNNGTC	1 cut(s)	22
EaeI	YGGCCR	3 cut(s)	687, 1251, 1606
Eam1104I	CTCTTC	2 cut(s)	627, 1533
Eam1105I	GACNNNNNGTC	1 cut(s)	22
EarI	CTCTTC	2 cut(s)	627, 1533
Eco130I	CCWWGG	3 cut(s)	134, 1609, 1682
Eco147I	AGGCCT	1 cut(s)	351
Eco47I	GGWCC	2 cut(s)	608, 1079
Eco57I	CTGAAG	1 cut(s)	1046
Eco81I	CCTNAGG	1 cut(s)	50
Eco88I	CYCGRG	1 cut(s)	644
EcoNI	CCTNNNNNAGG	2 cut(s)	133, 345
EcoO109I	RGGNCCY	1 cut(s)	641
EcoRI	GAATTC	1 cut(s)	1309
EcoRII	CCWGG	2 cut(s)	1081, 1175
EcoT14I	CCWWGG	3 cut(s)	134, 1609, 1682
EcoT22I	ATGCAT	1 cut(s)	1140
ErhI	CCWWGG	3 cut(s)	134, 1609, 1682
FaeI	CATG	9 cut(s)	311, 445, 684, 938, 1170, 1232, 1417, 1478, 1686
FaqI	GGGAC	2 cut(s)	189, 621
FatI	CATG	9 cut(s)	307, 441, 680, 934, 1166, 1228, 1413, 1474, 1682
FauI	CCCGC	1 cut(s)	1669
FbaI	TGATCA	1 cut(s)	1270
FokI	GGATG	7 cut(s)	14, 71, 697, 932, 1222, 1375, 1700
FspBI	CTAG	4 cut(s)	135, 635, 704, 1376
GsuI	CTGGAG	1 cut(s)	1104
HapII	CCGG	6 cut(s)	31, 173, 183, 237, 538, 1806
HgaI	GACGC	3 cut(s)	217, 423, 1604
Hin1I	GRCGYC	1 cut(s)	415
Hin1II	CATG	9 cut(s)	311, 445, 684, 938, 1170, 1232, 1417, 1478, 1686
HincII	GTYRAC	3 cut(s)	1107, 1587, 1803
HindII	GTYRAC	3 cut(s)	1107, 1587, 1803
HindIII	AAGCTT	1 cut(s)	1263
HinfI	GANTC	5 cut(s)	17, 23, 292, 902, 1633
HpaII	CCGG	6 cut(s)	31, 173, 183, 237, 538, 1806
HphI	GGTGA	2 cut(s)	91, 826
Hpy166II	GTNNAC	5 cut(s)	834, 836, 1107, 1587, 1803
Hpy188I	TCNGA	7 cut(s)	22, 299, 901, 1026, 1239, 1283, 1625
Hpy188III	TCNNGA	3 cut(s)	442, 941, 1501
Hpy8I	GTNNAC	5 cut(s)	834, 836, 1107, 1587, 1803
Hpy99I	CGWCG	1 cut(s)	1598
HpyAV	CCTTC	4 cut(s)	430, 1021, 1280, 1744
HpyCH4III	ACNGT	3 cut(s)	229, 601, 1559
HpyCH4IV	ACGT	3 cut(s)	122, 483, 652
HpyF3I	CTNAG	4 cut(s)	50, 218, 858, 951
HpySE526I	ACGT	3 cut(s)	122, 483, 652
Hsp92I	GRCGYC	1 cut(s)	415
Hsp92II	CATG	9 cut(s)	311, 445, 684, 938, 1170, 1232, 1417, 1478, 1686
KpnI	GGTACC	1 cut(s)	537
Ksp22I	TGATCA	1 cut(s)	1270
Kzo9I	GATC	7 cut(s)	434, 583, 631, 816, 922, 1234, 1270
LguI	GCTCTTC	2 cut(s)	627, 1533
LmnI	GCTCC	4 cut(s)	150, 511, 913, 1085
Lsp1109I	GCAGC	6 cut(s)	209, 274, 531, 560, 809, 1726
LweI	GCATC	3 cut(s)	49, 1335, 1678
MaeI	CTAG	4 cut(s)	135, 635, 704, 1376
MaeII	ACGT	3 cut(s)	122, 483, 652
MaeIII	GTNAC	6 cut(s)	79, 383, 695, 841, 1447, 1573
MalI	GATC	7 cut(s)	436, 585, 633, 818, 924, 1236, 1272
MbiI	CCGCTC	2 cut(s)	153, 871
MboI	GATC	7 cut(s)	434, 583, 631, 816, 922, 1234, 1270
MboII	GAAGA	7 cut(s)	117, 309, 420, 423, 614, 1064, 1520
MfeI	CAATTG	1 cut(s)	1641
MflI	RGATCY	3 cut(s)	434, 583, 1234
MhlI	GDGCHC	1 cut(s)	622
MlsI	TGGCCA	1 cut(s)	1608
MluNI	TGGCCA	1 cut(s)	1608
MlyI	GAGTC	3 cut(s)	11, 32, 286
MmeI	TCCRAC	1 cut(s)	466
Mox20I	TGGCCA	1 cut(s)	1608
Mph1103I	ATGCAT	1 cut(s)	1140
MscI	TGGCCA	1 cut(s)	1608
MseI	TTAA	7 cut(s)	275, 398, 464, 792, 1071, 1115, 1439
MslI	CAYNNNNRTG	1 cut(s)	32
Msp20I	TGGCCA	1 cut(s)	1608
MspA1I	CMGCKG	2 cut(s)	222, 1360
MspI	CCGG	6 cut(s)	31, 173, 183, 237, 538, 1806
MspR9I	CCNGG	6 cut(s)	32, 174, 183, 538, 1083, 1177
MunI	CAATTG	1 cut(s)	1641
Mva1269I	GAATGC	2 cut(s)	1138, 1292
MvaI	CCWGG	2 cut(s)	1083, 1177
MvnI	CGCG	1 cut(s)	1763
NciI	CCSGG	4 cut(s)	32, 174, 183, 538
NcoI	CCATGG	1 cut(s)	1682
NdeII	GATC	7 cut(s)	434, 583, 631, 816, 922, 1234, 1270
NlaIII	CATG	9 cut(s)	311, 445, 684, 938, 1170, 1232, 1417, 1478, 1686
NmeAIII	GCCGAG	1 cut(s)	1229
NmuCI	GTSAC	2 cut(s)	79, 383
NsiI	ATGCAT	1 cut(s)	1140
NspI	RCATGY	2 cut(s)	311, 1478
PaeI	GCATGC	1 cut(s)	311
PagI	TCATGA	1 cut(s)	441
PaqCI	CACCTGC	1 cut(s)	1589
PceI	AGGCCT	1 cut(s)	351
PciI	ACATGT	1 cut(s)	1474
PciSI	GCTCTTC	2 cut(s)	627, 1533
PctI	GAATGC	2 cut(s)	1138, 1292
PfeI	GAWTC	2 cut(s)	902, 1633
PflMI	CCANNNNNTGG	2 cut(s)	1363, 1615
PinAI	ACCGGT	1 cut(s)	1805
PleI	GAGTC	3 cut(s)	11, 31, 286
PpsI	GAGTC	3 cut(s)	11, 31, 286
PscI	ACATGT	1 cut(s)	1474
Psp6I	CCWGG	2 cut(s)	1081, 1175
PspGI	CCWGG	2 cut(s)	1081, 1175
PspPI	GGNCC	5 cut(s)	338, 608, 641, 1079, 1679
PstI	CTGCAG	1 cut(s)	481
PstNI	CAGNNNCTG	1 cut(s)	905
PsuI	RGATCY	3 cut(s)	434, 583, 1234
PvuII	CAGCTG	1 cut(s)	222
RsaI	GTAC	4 cut(s)	41, 535, 835, 1260
RsaNI	GTAC	4 cut(s)	40, 534, 834, 1259
RseI	CAYNNNNRTG	1 cut(s)	32
SapI	GCTCTTC	2 cut(s)	627, 1533
SaqAI	TTAA	7 cut(s)	275, 398, 464, 792, 1071, 1115, 1439
Sau3AI	GATC	7 cut(s)	434, 583, 631, 816, 922, 1234, 1270
Sau96I	GGNCC	5 cut(s)	338, 608, 641, 1079, 1679
SchI	GAGTC	3 cut(s)	11, 32, 286
ScrFI	CCNGG	6 cut(s)	32, 174, 183, 538, 1083, 1177
SduI	GDGCHC	1 cut(s)	622
SfaNI	GCATC	3 cut(s)	49, 1335, 1678
SfcI	CTRYAG	2 cut(s)	477, 1555
SgrAI	CRCCGGYG	1 cut(s)	236
SinI	GGWCC	2 cut(s)	608, 1079
SmiMI	CAYNNNNRTG	1 cut(s)	32
SmlI	CTYRAG	2 cut(s)	941, 1796
SmoI	CTYRAG	2 cut(s)	941, 1796
SpeI	ACTAGT	1 cut(s)	1375
SphI	GCATGC	1 cut(s)	311
SseBI	AGGCCT	1 cut(s)	351
SspMI	CTAG	4 cut(s)	135, 635, 704, 1376
StuI	AGGCCT	1 cut(s)	351
StyD4I	CCNGG	6 cut(s)	30, 172, 181, 536, 1081, 1175
StyI	CCWWGG	3 cut(s)	134, 1609, 1682
TaaI	ACNGT	3 cut(s)	229, 601, 1559
TaiI	ACGT	3 cut(s)	125, 486, 655
TaqI	TCGA	2 cut(s)	105, 1787
TatI	WGTACW	2 cut(s)	833, 1258
TauI	GCSGC	6 cut(s)	156, 159, 162, 172, 236, 1766
TfiI	GAWTC	2 cut(s)	902, 1633
Tru1I	TTAA	7 cut(s)	275, 398, 464, 792, 1071, 1115, 1439
Tru9I	TTAA	7 cut(s)	275, 398, 464, 792, 1071, 1115, 1439
TscAI	CASTG	6 cut(s)	606, 843, 880, 1095, 1149, 1564
TseFI	GTSAC	2 cut(s)	79, 383
TseI	GCWGC	6 cut(s)	222, 262, 519, 548, 797, 1739
Tsp45I	GTSAC	2 cut(s)	79, 383
TspDTI	ATGAA	5 cut(s)	309, 412, 1023, 1506, 1802
TspGWI	ACGGA	6 cut(s)	29, 98, 445, 1092, 1217, 1561
TspRI	CASTG	6 cut(s)	606, 843, 880, 1095, 1149, 1564
Van91I	CCANNNNNTGG	2 cut(s)	1363, 1615
VpaK11BI	GGWCC	2 cut(s)	608, 1079
XagI	CCTNNNNNAGG	2 cut(s)	133, 345
XapI	RAATTY	1 cut(s)	1309
XceI	RCATGY	2 cut(s)	311, 1478
XmaJI	CCTAGG	1 cut(s)	134
XspI	CTAG	4 cut(s)	135, 635, 704, 1376
Zsp2I	ATGCAT	1 cut(s)	1140

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.