MD13G1260600.v1.1

COBW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0021125
Physical Location & Seq
Forward (+)
31955284 .. 31958953
3670 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1260600.v1.1.491

Sequence Viewer

Length: 414 bp
ATGTTCGATCCTCCAGTTTCAGAGCAAAACATTGATCCAGTCCCGAGATCCACAAGAAGCAAAGACCATCATGAGGATCACCACACCCATGATCATGTTCATGATCCTAGTGTTTCTTCTGTCAGCATAGTTTGTGAAGGGAACTTAGATCTTGAAAAGGCTATCATATGGCTTGATACACTGTTGTTTGAACGAAGTGAGGACATATATCGTATGAAAGGTCTTCTATCAGTTCAAGGAATGAAAGAGAGATTTGTCTTTCAGGATTTTGGTGCTCTGAAAATGCCCACCCTGATATTTTTACACCAAGTGTATATGGCAACCCAGGCATTCCAACTTGCTCAGGGAAATACTTCATTTAAGGAAAAGCCTGAAATAATGTTGTCAAGTGCTATACTGAAGCTATCAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C cobW

Protein Analysis

138

Amino Acids

15.66

Weight (kDa)

5.68

Isoelectric Point (pI)

43.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CobW_C PF07683 38 - 91 1.4e-07 Cobalamin synthesis protein cobW C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021125)

Species Orthologous Gene IDs
malus_domestica MD09G1124100.v1.1 MD13G1260600.v1.1 MD17G1002200.v1.1
pyrus_communis pycom10g07090 pycom12g16410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 5 cut(s) 2, 29, 42, 84, 98
AdeI CACNNNGTG 1 cut(s) 310
AfiI CCNNNNNNNGG 1 cut(s) 73
AgsI TTSAA 3 cut(s) 155, 191, 236
AjnI CCWGG 1 cut(s) 324
AluBI AGCT 1 cut(s) 403
AluI AGCT 1 cut(s) 403
Alw21I GWGCWC 1 cut(s) 277
AlwI GGATC 5 cut(s) 2, 29, 42, 84, 98
Ama87I CYCGRG 1 cut(s) 43
Asp700I GAANNNNTTC 1 cut(s) 352
AsuHPI GGTGA 1 cut(s) 71
AvaI CYCGRG 1 cut(s) 43
BbsI GAAGAC 1 cut(s) 215
Bbv12I GWGCWC 1 cut(s) 277
BccI CCATC 1 cut(s) 75
BciT130I CCWGG 1 cut(s) 326
BclI TGATCA 1 cut(s) 91
BfaI CTAG 1 cut(s) 108
BglII AGATCT 1 cut(s) 148
Bme1390I CCNGG 1 cut(s) 326
BmeT110I CYCGRG 1 cut(s) 43
BmrFI CCNGG 1 cut(s) 326
BpiI GAAGAC 1 cut(s) 215
Bpu10I CCTNAGC 1 cut(s) 342
BsaJI CCNNGG 1 cut(s) 324
Bsc4I CCNNNNNNNGG 1 cut(s) 73
Bse1I ACTGG 2 cut(s) 14, 38
BseBI CCWGG 1 cut(s) 326
BseDI CCNNGG 1 cut(s) 324
BseLI CCNNNNNNNGG 1 cut(s) 73
BseMII CTCAG 1 cut(s) 356
BseNI ACTGG 2 cut(s) 14, 38
BsiHKAI GWGCWC 1 cut(s) 277
BsiHKCI CYCGRG 1 cut(s) 43
BslFI GGGAC 1 cut(s) 26
BslI CCNNNNNNNGG 1 cut(s) 73
BsmFI GGGAC 1 cut(s) 26
BsmI GAATGC 1 cut(s) 329
BsoBI CYCGRG 1 cut(s) 43
Bsp1286I GDGCHC 1 cut(s) 277
Bsp143I GATC 7 cut(s) 7, 34, 47, 76, 91, 103, 148
BspCNI CTCAG 1 cut(s) 355
BspHI TCATGA 2 cut(s) 70, 100
BspPI GGATC 5 cut(s) 2, 29, 42, 84, 98
BsrI ACTGG 2 cut(s) 14, 38
BssECI CCNNGG 1 cut(s) 324
BssMI GATC 7 cut(s) 7, 34, 47, 76, 91, 103, 148
Bst2UI CCWGG 1 cut(s) 326
Bst4CI ACNGT 1 cut(s) 183
BstDEI CTNAG 2 cut(s) 145, 342
BstKTI GATC 7 cut(s) 10, 37, 50, 79, 94, 106, 151
BstMBI GATC 7 cut(s) 7, 34, 47, 76, 91, 103, 148
BstMWI GCNNNNNNNGC 1 cut(s) 326
BstNI CCWGG 1 cut(s) 326
BstSCI CCNGG 1 cut(s) 324
BstV2I GAAGAC 1 cut(s) 215
BstX2I RGATCY 2 cut(s) 47, 148
BstYI RGATCY 2 cut(s) 47, 148
BtsIMutI CAGTG 1 cut(s) 179
CciI TCATGA 2 cut(s) 70, 100
CviAII CATG 4 cut(s) 71, 89, 95, 101
CviJI RGCY 4 cut(s) 161, 172, 370, 403
CviKI_1 RGCY 4 cut(s) 161, 172, 370, 403
DdeI CTNAG 2 cut(s) 145, 342
DpnI GATC 7 cut(s) 9, 36, 49, 78, 93, 105, 150
DpnII GATC 7 cut(s) 7, 34, 47, 76, 91, 103, 148
DraIII CACNNNGTG 1 cut(s) 310
Eco88I CYCGRG 1 cut(s) 43
EcoRII CCWGG 1 cut(s) 324
FaeI CATG 4 cut(s) 74, 92, 98, 104
FaqI GGGAC 1 cut(s) 26
FatI CATG 4 cut(s) 70, 88, 94, 100
FauNDI CATATG 1 cut(s) 167
FbaI TGATCA 1 cut(s) 91
FspBI CTAG 1 cut(s) 108
Hin1II CATG 4 cut(s) 74, 92, 98, 104
HphI GGTGA 1 cut(s) 71
Hpy188I TCNGA 2 cut(s) 22, 279
Hpy188III TCNNGA 5 cut(s) 43, 71, 101, 152, 263
HpyAV CCTTC 1 cut(s) 131
HpyCH4III ACNGT 1 cut(s) 183
HpyF10VI GCNNNNNNNGC 1 cut(s) 326
HpyF3I CTNAG 2 cut(s) 145, 342
Hsp92II CATG 4 cut(s) 74, 92, 98, 104
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 7 cut(s) 7, 34, 47, 76, 91, 103, 148
LpnPI CCDG 8 cut(s) 27, 51, 248, 305, 311, 329, 338, 384
MaeI CTAG 1 cut(s) 108
MalI GATC 7 cut(s) 9, 36, 49, 78, 93, 105, 150
MboI GATC 7 cut(s) 7, 34, 47, 76, 91, 103, 148
MboII GAAGA 2 cut(s) 108, 215
MflI RGATCY 2 cut(s) 47, 148
MhlI GDGCHC 1 cut(s) 277
MmeI TCCRAC 1 cut(s) 358
MnlI CCTC 3 cut(s) 21, 67, 193
MroXI GAANNNNTTC 1 cut(s) 352
MseI TTAA 1 cut(s) 360
MslI CAYNNNNRTG 3 cut(s) 87, 93, 99
MspR9I CCNGG 1 cut(s) 326
Mva1269I GAATGC 1 cut(s) 329
MvaI CCWGG 1 cut(s) 326
MwoI GCNNNNNNNGC 1 cut(s) 326
NdeI CATATG 1 cut(s) 167
NdeII GATC 7 cut(s) 7, 34, 47, 76, 91, 103, 148
NlaIII CATG 4 cut(s) 74, 92, 98, 104
PagI TCATGA 2 cut(s) 70, 100
PctI GAATGC 1 cut(s) 329
PdmI GAANNNNTTC 1 cut(s) 352
Psp6I CCWGG 1 cut(s) 324
PspGI CCWGG 1 cut(s) 324
PsuI RGATCY 2 cut(s) 47, 148
RseI CAYNNNNRTG 3 cut(s) 87, 93, 99
SaqAI TTAA 1 cut(s) 360
Sau3AI GATC 7 cut(s) 7, 34, 47, 76, 91, 103, 148
ScrFI CCNGG 1 cut(s) 326
SduI GDGCHC 1 cut(s) 277
SetI ASST 2 cut(s) 223, 405
SmiMI CAYNNNNRTG 3 cut(s) 87, 93, 99
SspMI CTAG 1 cut(s) 108
StyD4I CCNGG 1 cut(s) 324
TaaI ACNGT 1 cut(s) 183
TaqI TCGA 1 cut(s) 6
Tru1I TTAA 1 cut(s) 360
Tru9I TTAA 1 cut(s) 360
TscAI CASTG 1 cut(s) 186
TspDTI ATGAA 4 cut(s) 89, 230, 257, 345
TspRI CASTG 1 cut(s) 186
XmnI GAANNNNTTC 1 cut(s) 352
XspI CTAG 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.