Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0015089

Physical Location & Seq

Genomic Coordinates Forward (+)

37345962 .. 37347260

Gene Structure 1299 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1274600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1299 bp

ATGCCGTCTGCATTGGAGGATGAGTTGTTCCCGTCGACGCCCGGAAAGTTCAAAATCGAGCGCAGCCACGCCATGAACCGCCAGTTCCACCGCTGTTTCGCCTCCACAAGCACCATGTTCTTGTGGGCCTTATTTCTTATCGCCCTGACGGCGTCCTATTTGAGTTTTCAGAGCTTCGTCGACTCCGGCAGCCGCTACTTCTCCGCCTCCTGGGGCGGCATCCAGTGGGAGAAGCAGGTCCGCAACTCCGCCCAGATCCACCGCTCCGGCGGCATGTCCGTCCTCGTCACCGGCGCAGCCGGTTTCGTCGGAACCCACGTCTCCCTGGGCCTCAAAAAGCGCGGAGACGGAGTCGTCGGAATTGACAACTTCAACAGCTACTACGACCCGTCGCTGAAGAAGGCTCGCCGGTCACTCCTCAAGACCCACGGCGTTTTCATCGTCGAGGGCGACATAAACGACAATCGCCTTCTGGACAAGCTATTCGATACAGTGGCGTTCACCCATGTGATGCATTTGGCGGCTCAGGCCGGCGTCCGGTACGCCATGGAGAACCCCCATTCTTACGTCCACAGCAACATCGCCGGCCTCGTGACGCTTCTCGAAGTCTGCAAATCGGCCAATCCTCAGCCGTCCATTGTTTGGGCCTCGTCCAGCTCTGTCTACGGCCTGAACGACAACGTCCCATTCTCCGAATCAGACCGGACTGACCAGCCGGCTAGCCTCTACGCCGCCACCAAGAAAGCCGGCGAAGAAATTACCCACACTTACAACCATATTTATGGCCTGTCAATTACCGGGTTAAGATTTTTCACTGTGTACGGACCGTGGGGCCGACCCGACATGGCATATTTCTCTTTCACCCGCAACATCCTCCAGGGGAAGCCCATCACCATTTACAGGGGCAAGAACCGGGTGGACTTGGCCCGGGATTTCACGTATATTGACGACATCGTGAAGGGTTGTTTGGGGTCATTGGATACGTCCGGAAAGAGTACCGGGTCGGGTGGGAAGAAGCGGGGAGCGGCGCCGTACCGGATCTTTAATTTGGGGAACACATCACCGGTGACAGTGCCCACACTCGTCAACATCTTGGAGCGGCATTTGAGGACGAAGGCGAAGAAAAATGTAGTGGAGATGCCTGGAAACGGCGACGTTCCGTTCACACACGCGAATATAAGCTTGGCCCGAAGAGAACTCGGGTATAAGCCCACAACCGATTTGCAAACCGGGTTGAAGAAGTTTGTTAGGTGGTACCTTTCGTATTACGGCTACAATCACGGCAAGCCTGTAAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005975 GO:0005996 GO:0006063 GO:0006082 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009987 GO:0012505 GO:0016051 GO:0016053 GO:0016853 GO:0016854 GO:0016857 GO:0019586 GO:0019752 GO:0031410 GO:0031982 GO:0031984 GO:0032787 GO:0033481 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046364 GO:0046394 GO:0050378 GO:0050829 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0072330 GO:0097708 GO:0098542 GO:0098791 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

Epimerase GDP_Man_Dehyd

Protein Analysis

433

Amino Acids

47.9

Weight (kDa)

9.63

Isoelectric Point (pI)

38.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RmlD_sub_bind	PF04321	92 - 275	3.6e-13	RmlD substrate binding domain
Epimerase	PF01370	94 - 328	2.1e-50	NAD dependent epimerase/dehydratase family
Polysacc_synt_2	PF02719	94 - 299	1.7e-08	Polysaccharide biosynthesis protein
GDP_Man_Dehyd	PF16363	95 - 414	2e-48	GDP-mannose 4,6 dehydratase
3Beta_HSD	PF01073	95 - 318	6.2e-13	3-beta hydroxysteroid dehydrogenase/isomerase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015089)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G30440
fragaria_vesca	FvH4_4g10050
malus_domestica	MD13G1274600.v1.1 MD16G1260200.v1.1
prunus_persica	Prupe.1G099000_v2.0.a1
pyrus_communis	pycom13g28710
rosa_chinensis	RchiOBHm_Chr4g0405111
rosa_laevigata	RLG00000008874
rosa_multiflora	Rmu_sc0001744.1_g000052 Rmu_ssc0000255.1_g000051
rosa_roxburghii	Rroxscaffold_5G00349280
rosa_rugosa	Rorug04G0053500
rosa_samantha	Rh4BG122100 Rh4CG135800 Rh4DG122300
rosa_wichuraiana	Rw4G010380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	353
Acc36I	ACCTGC	1 cut(s)	226
Acc65I	GGTACC	1 cut(s)	1254
AccB1I	GGYRCC	2 cut(s)	1027, 1254
AccB7I	CCANNNNNTGG	1 cut(s)	642
AccBSI	CCGCTC	3 cut(s)	264, 1025, 1099
AccI	GTMKAC	3 cut(s)	35, 180, 663
AccII	CGCG	2 cut(s)	342, 1172
AccIII	TCCGGA	1 cut(s)	986
AclWI	GGATC	2 cut(s)	250, 1046
AcoI	YGGCCR	1 cut(s)	618
AcuI	CTGAAG	1 cut(s)	416
AcyI	GRCGYC	4 cut(s)	38, 152, 534, 1028
AfaI	GTAC	5 cut(s)	542, 821, 997, 1034, 1256
AfiI	CCNNNNNNNGG	5 cut(s)	210, 537, 642, 900, 1148
AgeI	ACCGGT	1 cut(s)	1063
AgsI	TTSAA	3 cut(s)	52, 373, 1237
AjiI	CACGTC	1 cut(s)	319
AjnI	CCWGG	4 cut(s)	209, 324, 876, 1141
AjuI	GAANNNNNNNTTGG	4 cut(s)	950, 982, 1166, 1198
AleI	CACNNNNGTG	1 cut(s)	506
AloI	GAACNNNNNNTCC	2 cut(s)	11, 43
AluBI	AGCT	5 cut(s)	174, 378, 481, 657, 1182
AluI	AGCT	5 cut(s)	174, 378, 481, 657, 1182
Alw26I	GTCTC	2 cut(s)	325, 339
AlwI	GGATC	2 cut(s)	250, 1046
Ama87I	CYCGRG	2 cut(s)	927, 1199
Aor13HI	TCCGGA	1 cut(s)	986
ApeKI	GCWGC	3 cut(s)	63, 189, 296
ArsI	GACNNNNNNTTYG	2 cut(s)	467, 499
AsiGI	ACCGGT	1 cut(s)	1063
Asp718I	GGTACC	1 cut(s)	1254
AspLEI	GCGC	4 cut(s)	63, 296, 342, 1030
AspS9I	GGNCC	8 cut(s)	126, 238, 328, 645, 824, 832, 925, 1186
AsuC2I	CCSGG	7 cut(s)	42, 799, 914, 928, 929, 1000, 1231
AsuHPI	GGTGA	6 cut(s)	280, 493, 853, 883, 1053, 1078
AsuNHI	GCTAGC	1 cut(s)	719
AvaI	CYCGRG	2 cut(s)	927, 1199
AvaII	GGWCC	2 cut(s)	238, 824
BaeGI	GKGCMC	1 cut(s)	1077
BanI	GGYRCC	2 cut(s)	1027, 1254
BauI	CACGAG	1 cut(s)	590
BbvCI	CCTCAGC	1 cut(s)	627
BbvI	GCAGC	3 cut(s)	75, 201, 308
BccI	CCATC	1 cut(s)	896
BceAI	ACGGC	8 cut(s)	165, 445, 616, 682, 1015, 1165, 1285, 1297
BciT130I	CCWGG	4 cut(s)	211, 326, 878, 1143
BciVI	GTATCC	1 cut(s)	973
BcnI	CCSGG	7 cut(s)	42, 799, 914, 928, 929, 1000, 1231
BcoDI	GTCTC	2 cut(s)	325, 339
BfaI	CTAG	1 cut(s)	720
BfoI	RGCGCY	1 cut(s)	1031
BfuAI	ACCTGC	1 cut(s)	226
BfuI	GTATCC	1 cut(s)	973
BglI	GCCNNNNNGGC	1 cut(s)	149
Bme18I	GGWCC	2 cut(s)	238, 824
BmeT110I	CYCGRG	2 cut(s)	927, 1199
BmgBI	CACGTC	1 cut(s)	319
BmgT120I	GGNCC	8 cut(s)	126, 238, 328, 645, 824, 832, 925, 1186
BmiI	GGNNCC	4 cut(s)	313, 833, 1029, 1256
BmsI	GCATC	3 cut(s)	228, 501, 1128
BmtI	GCTAGC	1 cut(s)	723
BpmI	CTGGAG	1 cut(s)	860
Bpu10I	CCTNAGC	2 cut(s)	525, 627
BpuEI	CTTGAG	1 cut(s)	404
BpuMI	CCSGG	7 cut(s)	42, 799, 914, 928, 929, 1000, 1231
BsaAI	YACGTR	1 cut(s)	939
BsaHI	GRCGYC	4 cut(s)	38, 152, 534, 1028
BsaJI	CCNNGG	8 cut(s)	210, 324, 325, 427, 546, 827, 877, 927
BsaWI	WCCGGW	5 cut(s)	537, 702, 986, 1035, 1063
BsaXI	ACNNNNNCTCC	4 cut(s)	8, 38, 336, 366
Bsc4I	CCNNNNNNNGG	5 cut(s)	210, 537, 642, 900, 1148
Bse118I	RCCGGY	8 cut(s)	290, 299, 408, 530, 584, 715, 746, 1063
Bse1I	ACTGG	2 cut(s)	82, 223
BseAI	TCCGGA	1 cut(s)	986
BseBI	CCWGG	4 cut(s)	211, 326, 878, 1143
BseDI	CCNNGG	8 cut(s)	210, 324, 325, 427, 546, 827, 877, 927
BseGI	GGATG	3 cut(s)	25, 219, 870
BseLI	CCNNNNNNNGG	5 cut(s)	210, 537, 642, 900, 1148
BseMII	CTCAG	2 cut(s)	539, 641
BseNI	ACTGG	2 cut(s)	82, 223
BseRI	GAGGAG	1 cut(s)	407
BseSI	GKGCMC	1 cut(s)	1077
BseXI	GCAGC	3 cut(s)	75, 201, 308
Bsh1236I	CGCG	2 cut(s)	342, 1172
BshNI	GGYRCC	2 cut(s)	1027, 1254
BshTI	ACCGGT	1 cut(s)	1063
BsiHKCI	CYCGRG	2 cut(s)	927, 1199
BslFI	GGGAC	1 cut(s)	668
BslI	CCNNNNNNNGG	5 cut(s)	210, 537, 642, 900, 1148
BsmAI	GTCTC	2 cut(s)	325, 339
BsmBI	CGTCTC	2 cut(s)	325, 339
BsmFI	GGGAC	1 cut(s)	668
BsoBI	CYCGRG	2 cut(s)	927, 1199
Bsp1286I	GDGCHC	1 cut(s)	1077
Bsp13I	TCCGGA	1 cut(s)	986
Bsp143I	GATC	2 cut(s)	255, 1038
Bsp19I	CCATGG	1 cut(s)	546
BspCNI	CTCAG	2 cut(s)	538, 640
BspEI	TCCGGA	1 cut(s)	986
BspFNI	CGCG	2 cut(s)	342, 1172
BspLI	GGNNCC	4 cut(s)	313, 833, 1029, 1256
BspMI	ACCTGC	1 cut(s)	226
BspOI	GCTAGC	1 cut(s)	723
BspPI	GGATC	2 cut(s)	250, 1046
BspT107I	GGYRCC	2 cut(s)	1027, 1254
BsrBI	CCGCTC	3 cut(s)	264, 1025, 1099
BsrFI	RCCGGY	8 cut(s)	290, 299, 408, 530, 584, 715, 746, 1063
BsrI	ACTGG	2 cut(s)	82, 223
BssAI	RCCGGY	8 cut(s)	290, 299, 408, 530, 584, 715, 746, 1063
BssECI	CCNNGG	8 cut(s)	210, 324, 325, 427, 546, 827, 877, 927
BssMI	GATC	2 cut(s)	255, 1038
BssNI	GRCGYC	4 cut(s)	38, 152, 534, 1028
BssSI	CACGAG	1 cut(s)	590
BssT1I	CCWWGG	1 cut(s)	546
Bst2BI	CACGAG	1 cut(s)	590
Bst2UI	CCWGG	4 cut(s)	211, 326, 878, 1143
Bst4CI	ACNGT	4 cut(s)	493, 817, 828, 1072
Bst6I	CTCTTC	1 cut(s)	1186
BstACI	GRCGYC	4 cut(s)	38, 152, 534, 1028
BstBAI	YACGTR	1 cut(s)	939
BstC8I	GCNNGC	7 cut(s)	406, 532, 586, 717, 721, 748, 1286
BstDEI	CTNAG	2 cut(s)	525, 627
BstDSI	CCRYGG	3 cut(s)	427, 546, 827
BstF5I	GGATG	3 cut(s)	25, 219, 870
BstFNI	CGCG	2 cut(s)	342, 1172
BstH2I	RGCGCY	1 cut(s)	1031
BstHHI	GCGC	4 cut(s)	63, 296, 342, 1030
BstKTI	GATC	2 cut(s)	258, 1041
BstMAI	GTCTC	2 cut(s)	325, 339
BstMBI	GATC	2 cut(s)	255, 1038
BstMWI	GCNNNNNNNGC	3 cut(s)	149, 270, 527
BstNI	CCWGG	4 cut(s)	211, 326, 878, 1143
BstNSI	RCATGY	1 cut(s)	277
BstSLI	GKGCMC	1 cut(s)	1077
BstUI	CGCG	2 cut(s)	342, 1172
BstV1I	GCAGC	3 cut(s)	75, 201, 308
BstX2I	RGATCY	2 cut(s)	255, 1038
BstXI	CCANNNNNNTGG	1 cut(s)	782
BstYI	RGATCY	2 cut(s)	255, 1038
BsuI	GTATCC	1 cut(s)	973
BtgI	CCRYGG	3 cut(s)	427, 546, 827
BtgZI	GCGATG	1 cut(s)	565
BtrI	CACGTC	1 cut(s)	319
BtsCI	GGATG	3 cut(s)	25, 219, 870
BtsIMutI	CAGTG	4 cut(s)	230, 498, 813, 1077
BveI	ACCTGC	1 cut(s)	226
Cac8I	GCNNGC	7 cut(s)	406, 532, 586, 717, 721, 748, 1286
CfoI	GCGC	4 cut(s)	63, 296, 342, 1030
Cfr10I	RCCGGY	8 cut(s)	290, 299, 408, 530, 584, 715, 746, 1063
Cfr13I	GGNCC	8 cut(s)	126, 238, 328, 645, 824, 832, 925, 1186
Cfr9I	CCCGGG	1 cut(s)	927
CpoI	CGGWCCG	1 cut(s)	824
CseI	GACGC	4 cut(s)	46, 141, 523, 604
Csp6I	GTAC	5 cut(s)	541, 820, 996, 1033, 1255
CspAI	ACCGGT	1 cut(s)	1063
CspI	CGGWCCG	1 cut(s)	824
CviAII	CATG	6 cut(s)	73, 115, 274, 506, 547, 844
CviQI	GTAC	5 cut(s)	541, 820, 996, 1033, 1255
DdeI	CTNAG	2 cut(s)	525, 627
DinI	GGCGCC	1 cut(s)	1029
DpnI	GATC	2 cut(s)	257, 1040
DpnII	GATC	2 cut(s)	255, 1038
DrdI	GACNNNNNNGTC	1 cut(s)	353
DseDI	GACNNNNNNGTC	1 cut(s)	353
EaeI	YGGCCR	1 cut(s)	618
Eam1104I	CTCTTC	1 cut(s)	1186
EarI	CTCTTC	1 cut(s)	1186
EciI	GGCGGA	2 cut(s)	193, 238
Eco130I	CCWWGG	1 cut(s)	546
Eco47I	GGWCC	2 cut(s)	238, 824
Eco57I	CTGAAG	1 cut(s)	416
Eco88I	CYCGRG	2 cut(s)	927, 1199
EcoRII	CCWGG	4 cut(s)	209, 324, 876, 1141
EcoT14I	CCWWGG	1 cut(s)	546
EcoT22I	ATGCAT	1 cut(s)	516
EgeI	GGCGCC	1 cut(s)	1029
EheI	GGCGCC	1 cut(s)	1029
ErhI	CCWWGG	1 cut(s)	546
Esp3I	CGTCTC	2 cut(s)	325, 339
FaeI	CATG	6 cut(s)	76, 118, 277, 509, 550, 847
FaqI	GGGAC	1 cut(s)	668
FatI	CATG	6 cut(s)	72, 114, 273, 505, 546, 843
FauI	CCCGC	2 cut(s)	872, 1011
FblI	GTMKAC	3 cut(s)	35, 180, 663
FokI	GGATG	3 cut(s)	32, 206, 857
FspBI	CTAG	1 cut(s)	720
GlaI	GCGC	4 cut(s)	62, 295, 341, 1029
GsuI	CTGGAG	1 cut(s)	860
HaeII	RGCGCY	1 cut(s)	1031
HgaI	GACGC	4 cut(s)	46, 141, 523, 604
HhaI	GCGC	4 cut(s)	63, 296, 342, 1030
Hin1I	GRCGYC	4 cut(s)	38, 152, 534, 1028
Hin1II	CATG	6 cut(s)	76, 118, 277, 509, 550, 847
Hin6I	GCGC	4 cut(s)	61, 294, 340, 1028
HinP1I	GCGC	4 cut(s)	61, 294, 340, 1028
HincII	GTYRAC	3 cut(s)	36, 181, 1087
HindII	GTYRAC	3 cut(s)	36, 181, 1087
HindIII	AAGCTT	1 cut(s)	1180
HinfI	GANTC	3 cut(s)	182, 351, 695
HphI	GGTGA	6 cut(s)	280, 493, 853, 883, 1053, 1078
Hpy166II	GTNNAC	9 cut(s)	36, 181, 501, 571, 664, 820, 919, 1087, 1164
Hpy188I	TCNGA	5 cut(s)	171, 311, 359, 694, 700
Hpy188III	TCNNGA	6 cut(s)	421, 473, 592, 602, 955, 987
Hpy8I	GTNNAC	9 cut(s)	36, 181, 501, 571, 664, 820, 919, 1087, 1164
Hpy99I	CGWCG	8 cut(s)	37, 40, 182, 311, 359, 394, 446, 1157
HpyAV	CCTTC	4 cut(s)	394, 479, 952, 1108
HpyCH4III	ACNGT	4 cut(s)	493, 817, 828, 1072
HpyCH4IV	ACGT	6 cut(s)	318, 567, 681, 938, 983, 1155
HpyCH4V	TGCA	4 cut(s)	11, 514, 612, 1225
HpyF10VI	GCNNNNNNNGC	3 cut(s)	149, 270, 527
HpyF3I	CTNAG	2 cut(s)	525, 627
HpySE526I	ACGT	6 cut(s)	318, 567, 681, 938, 983, 1155
Hsp92I	GRCGYC	4 cut(s)	38, 152, 534, 1028
Hsp92II	CATG	6 cut(s)	76, 118, 277, 509, 550, 847
HspAI	GCGC	4 cut(s)	61, 294, 340, 1028
KasI	GGCGCC	1 cut(s)	1027
Kpn2I	TCCGGA	1 cut(s)	986
KpnI	GGTACC	1 cut(s)	1258
KroI	GCCGGC	4 cut(s)	530, 584, 715, 746
KroNI	GCCGGC	4 cut(s)	532, 586, 717, 748
Kzo9I	GATC	2 cut(s)	255, 1038
LmnI	GCTCC	3 cut(s)	269, 1022, 1096
Lsp1109I	GCAGC	3 cut(s)	75, 201, 308
LweI	GCATC	3 cut(s)	228, 501, 1128
MaeI	CTAG	1 cut(s)	720
MaeII	ACGT	6 cut(s)	318, 567, 681, 938, 983, 1155
MaeIII	GTNAC	4 cut(s)	286, 411, 592, 1066
MalI	GATC	2 cut(s)	257, 1040
MbiI	CCGCTC	3 cut(s)	264, 1025, 1099
MboI	GATC	2 cut(s)	255, 1038
MboII	GAAGA	6 cut(s)	409, 764, 1024, 1132, 1203, 1249
MflI	RGATCY	2 cut(s)	255, 1038
MhlI	GDGCHC	1 cut(s)	1077
MluCI	AATT	5 cut(s)	360, 756, 792, 1045, 1294
Mly113I	GGCGCC	1 cut(s)	1028
MlyI	GAGTC	2 cut(s)	176, 360
MmeI	TCCRAC	2 cut(s)	289, 337
Mph1103I	ATGCAT	1 cut(s)	516
MroI	TCCGGA	1 cut(s)	986
MroNI	GCCGGC	4 cut(s)	530, 584, 715, 746
MseI	TTAA	3 cut(s)	803, 1044, 1297
MslI	CAYNNNNRTG	2 cut(s)	506, 780
MspA1I	CMGCKG	1 cut(s)	93
MvaI	CCWGG	4 cut(s)	211, 326, 878, 1143
MvnI	CGCG	2 cut(s)	342, 1172
MwoI	GCNNNNNNNGC	3 cut(s)	149, 270, 527
NaeI	GCCGGC	4 cut(s)	532, 586, 717, 748
NarI	GGCGCC	1 cut(s)	1028
NciI	CCSGG	7 cut(s)	42, 799, 914, 928, 929, 1000, 1231
NcoI	CCATGG	1 cut(s)	546
NdeII	GATC	2 cut(s)	255, 1038
NgoMIV	GCCGGC	4 cut(s)	530, 584, 715, 746
NheI	GCTAGC	1 cut(s)	719
NlaIII	CATG	6 cut(s)	76, 118, 277, 509, 550, 847
NlaIV	GGNNCC	4 cut(s)	313, 833, 1029, 1256
NmuCI	GTSAC	4 cut(s)	286, 411, 592, 1066
NsiI	ATGCAT	1 cut(s)	516
NspI	RCATGY	1 cut(s)	277
OliI	CACNNNNGTG	1 cut(s)	506
PasI	CCCWGGG	1 cut(s)	325
PcsI	WCGNNNNNNNCGW	4 cut(s)	315, 447, 588, 672
PdiI	GCCGGC	4 cut(s)	532, 586, 717, 748
PfeI	GAWTC	1 cut(s)	695
PflFI	GACNNNGTC	3 cut(s)	151, 350, 680
PflMI	CCANNNNNTGG	1 cut(s)	642
PinAI	ACCGGT	1 cut(s)	1063
PleI	GAGTC	2 cut(s)	176, 359
PluTI	GGCGCC	1 cut(s)	1031
PpsI	GAGTC	2 cut(s)	176, 359
Ppu21I	YACGTR	1 cut(s)	939
Psp6I	CCWGG	4 cut(s)	209, 324, 876, 1141
PspGI	CCWGG	4 cut(s)	209, 324, 876, 1141
PspN4I	GGNNCC	4 cut(s)	313, 833, 1029, 1256
PspPI	GGNCC	8 cut(s)	126, 238, 328, 645, 824, 832, 925, 1186
PsuI	RGATCY	2 cut(s)	255, 1038
PsyI	GACNNNGTC	3 cut(s)	151, 350, 680
RsaI	GTAC	5 cut(s)	542, 821, 997, 1034, 1256
RsaNI	GTAC	5 cut(s)	541, 820, 996, 1033, 1255
RseI	CAYNNNNRTG	2 cut(s)	506, 780
Rsr2I	CGGWCCG	1 cut(s)	824
RsrII	CGGWCCG	1 cut(s)	824
SalI	GTCGAC	2 cut(s)	34, 179
SaqAI	TTAA	3 cut(s)	803, 1044, 1297
Sau3AI	GATC	2 cut(s)	255, 1038
Sau96I	GGNCC	8 cut(s)	126, 238, 328, 645, 824, 832, 925, 1186
SchI	GAGTC	2 cut(s)	176, 360
SduI	GDGCHC	1 cut(s)	1077
SfaNI	GCATC	3 cut(s)	228, 501, 1128
SfoI	GGCGCC	1 cut(s)	1029
SgrAI	CRCCGGYG	2 cut(s)	290, 1063
SgrDI	CGTCGACG	1 cut(s)	34
SinI	GGWCC	2 cut(s)	238, 824
SmaI	CCCGGG	1 cut(s)	929
SmiMI	CAYNNNNRTG	2 cut(s)	506, 780
SmlI	CTYRAG	1 cut(s)	419
SmoI	CTYRAG	1 cut(s)	419
Sse9I	AATT	5 cut(s)	360, 756, 792, 1045, 1294
SspDI	GGCGCC	1 cut(s)	1027
SspMI	CTAG	1 cut(s)	720
StyI	CCWWGG	1 cut(s)	546
TaaI	ACNGT	4 cut(s)	493, 817, 828, 1072
TaiI	ACGT	6 cut(s)	321, 570, 684, 941, 986, 1158
TaqI	TCGA	6 cut(s)	35, 57, 180, 444, 486, 603
TasI	AATT	5 cut(s)	360, 756, 792, 1045, 1294
TauI	GCSGC	7 cut(s)	195, 219, 273, 524, 734, 1028, 1102
TfiI	GAWTC	1 cut(s)	695
Tru1I	TTAA	3 cut(s)	803, 1044, 1297
Tru9I	TTAA	3 cut(s)	803, 1044, 1297
TscAI	CASTG	4 cut(s)	230, 498, 820, 1077
TseFI	GTSAC	4 cut(s)	286, 411, 592, 1066
TseI	GCWGC	3 cut(s)	63, 189, 296
Tsp45I	GTSAC	4 cut(s)	286, 411, 592, 1066
TspDTI	ATGAA	2 cut(s)	89, 427
TspGWI	ACGGA	4 cut(s)	268, 363, 837, 1149
TspMI	CCCGGG	1 cut(s)	927
TspRI	CASTG	4 cut(s)	230, 498, 820, 1077
Tth111I	GACNNNGTC	3 cut(s)	151, 350, 680
Van91I	CCANNNNNTGG	1 cut(s)	642
VpaK11BI	GGWCC	2 cut(s)	238, 824
XceI	RCATGY	1 cut(s)	277
XmaI	CCCGGG	1 cut(s)	927
XmiI	GTMKAC	3 cut(s)	35, 180, 663
XspI	CTAG	1 cut(s)	720
Zsp2I	ATGCAT	1 cut(s)	516

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.