Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0015089

Physical Location & Seq

Genomic Coordinates Forward (+)

32030303 .. 32031601

Gene Structure 1299 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1260200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1299 bp

ATGTCGTCTTCATTGGAAGATGAGTTGTTCCCCTCGACGCCCGGCAAGTTCAAAATCGAGCGGAGCCACGCCATGAACCGCCAGTTCCACCGCTGCTTCGCCTCGACAAGCACCATGTTCTTGTGGGCCCTATTTCTCATCGCCTTGACCGCGTCGTATTTGAGTTTTCAGAGCTTCATTGACTCCAGCAGCCGCTACTTCTCCGCCTCCTGGGGTGGCATCCAGTGGGAGAAGCAGGTTCGCAACTCTGCCCAGATCCACCGCTCCGGCGGCATGTCCGTCCTCATCACCGGCGCCGCCGGATTCGTCGGGAGCCACGTCTCTCTAGCCCTCAAAAAGCGCGGAGACGGCGTCGTCGGAATTGACAACTTCAACAGCTACTACGACCCGTCGTTGAAGGAAGCTCGCAGGTCGCTCCTCAAGACCCACGACATTTTCATCGTCGAGGGCGACATAAACGACAACCGCCTCCTCGACAAGTTATTCGACACGGTGGCGTTCACCCACGTGATGCACTTGGCGGCTCAAGCCGGCGTCCGGTACGCCATGGAAAACCCTCATTCCTACGTCCACAGCAATATCGCCGGCCTCGTCACACTTCTTGAAGTCTGCAAATCGGCCAATCCTCAGCCGTCCATCGTTTGGGCCTCGTCCAGCTCTGTCTACGGCTTAAACGACAACGTTCCGTTCTCCGAATTGCACCGTACGGACCAGCCGGCCAGCCTCTATGCCGCCACCAAGAAAGCCGGTGAAGAAATTACCCACACTTACAACCATATTTACGGCCTGTCAATTACCGGTTTGAGATTTTTCACCGTGTACGGACCCTGGGGACGACCCGACATGGCCTATTTTTCTTTCACCCGCAACATCCTCCAAGGAAAGCCCATCACCATTTACCGGGGCAAGAACGGGATCGACTTGGCGAGGGATTTCACATATATTGACGACATCGTGAAGGGCTGTTTGGGGTCGCTGGATACATCCGGGAAGAGTACCGGGTCGGGTGGGAAGAAGCGGGGATTGGCGCCGTACCGGATCTTTAATTTGGGGAACACATCACCGGTGACAGTGCCGACACTTGTCAACATCTTGGAGAGGCATTTGAAGACGAAGGCAAAGAAAAAGGTGGTGGACATGCCTGGAAACGGCGACGTTCCGTTCACGCACGCGAATATAAGTTCGGCCGGAAGAGAACTCGGGTACAAGCCCACAACCAATTTGCAGAGCGGGTTGAACAAGTTTGTTAGATGGTACCTTTCTTATTACGGCTACAATCGTGGCAAGCCTGTAAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005975 GO:0005996 GO:0006063 GO:0006082 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009987 GO:0012505 GO:0016051 GO:0016053 GO:0016853 GO:0016854 GO:0016857 GO:0019586 GO:0019752 GO:0031410 GO:0031982 GO:0031984 GO:0032787 GO:0033481 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046364 GO:0046394 GO:0050378 GO:0050829 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0072330 GO:0097708 GO:0098542 GO:0098791 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

Epimerase GDP_Man_Dehyd

Protein Analysis

433

Amino Acids

47.88

Weight (kDa)

9.54

Isoelectric Point (pI)

39.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RmlD_sub_bind	PF04321	92 - 276	2.3e-12	RmlD substrate binding domain
Epimerase	PF01370	94 - 328	4.1e-51	NAD dependent epimerase/dehydratase family
Polysacc_synt_2	PF02719	94 - 215	7.9e-09	Polysaccharide biosynthesis protein
GDP_Man_Dehyd	PF16363	95 - 414	1.6e-49	GDP-mannose 4,6 dehydratase
3Beta_HSD	PF01073	95 - 316	1.6e-13	3-beta hydroxysteroid dehydrogenase/isomerase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015089)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G30440
fragaria_vesca	FvH4_4g10050
malus_domestica	MD13G1274600.v1.1 MD16G1260200.v1.1
prunus_persica	Prupe.1G099000_v2.0.a1
pyrus_communis	pycom13g28710
rosa_chinensis	RchiOBHm_Chr4g0405111
rosa_laevigata	RLG00000008874
rosa_multiflora	Rmu_sc0001744.1_g000052 Rmu_ssc0000255.1_g000051
rosa_roxburghii	Rroxscaffold_5G00349280
rosa_rugosa	Rorug04G0053500
rosa_samantha	Rh4BG122100 Rh4CG135800 Rh4DG122300
rosa_wichuraiana	Rw4G010380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	353
Acc36I	ACCTGC	2 cut(s)	226, 399
Acc65I	GGTACC	1 cut(s)	1254
AccB1I	GGYRCC	3 cut(s)	293, 1027, 1254
AccB7I	CCANNNNNTGG	1 cut(s)	642
AccBSI	CCGCTC	3 cut(s)	61, 264, 1230
AccI	GTMKAC	1 cut(s)	663
AccII	CGCG	3 cut(s)	152, 342, 1172
AclI	AACGTT	1 cut(s)	681
AclWI	GGATC	3 cut(s)	250, 923, 1046
AcoI	YGGCCR	3 cut(s)	618, 717, 1185
AcvI	CACGTG	1 cut(s)	508
AcyI	GRCGYC	5 cut(s)	38, 294, 351, 534, 1028
AfaI	GTAC	7 cut(s)	542, 706, 821, 997, 1034, 1205, 1256
AfiI	CCNNNNNNNGG	5 cut(s)	210, 537, 642, 900, 1148
AgeI	ACCGGT	2 cut(s)	797, 1063
AgsI	TTSAA	6 cut(s)	52, 373, 397, 605, 1108, 1237
AjiI	CACGTC	1 cut(s)	319
AjnI	CCWGG	3 cut(s)	209, 827, 1141
AjuI	GAANNNNNNNTTGG	4 cut(s)	950, 982, 1007, 1039
AleI	CACNNNNGTG	1 cut(s)	506
AluBI	AGCT	4 cut(s)	174, 378, 404, 657
AluI	AGCT	4 cut(s)	174, 378, 404, 657
Alw26I	GTCTC	2 cut(s)	325, 339
AlwI	GGATC	3 cut(s)	250, 923, 1046
Ama87I	CYCGRG	1 cut(s)	1199
AoxI	GGCC	8 cut(s)	126, 586, 618, 645, 717, 784, 846, 1185
ApaI	GGGCCC	1 cut(s)	130
ApeKI	GCWGC	2 cut(s)	93, 189
ArsI	GACNNNNNNTTYG	2 cut(s)	467, 499
AsiGI	ACCGGT	2 cut(s)	797, 1063
Asp718I	GGTACC	1 cut(s)	1254
AspLEI	GCGC	3 cut(s)	296, 342, 1030
AspS9I	GGNCC	5 cut(s)	126, 127, 645, 709, 824
AsuC2I	CCSGG	4 cut(s)	42, 902, 988, 1000
AsuHPI	GGTGA	8 cut(s)	280, 493, 761, 805, 853, 883, 1053, 1078
AvaI	CYCGRG	1 cut(s)	1199
AvaII	GGWCC	2 cut(s)	709, 824
BaeGI	GKGCMC	1 cut(s)	130
BanI	GGYRCC	3 cut(s)	293, 1027, 1254
BanII	GRGCYC	1 cut(s)	130
BbrPI	CACGTG	1 cut(s)	508
BbsI	GAAGAC	1 cut(s)	1115
BbvCI	CCTCAGC	1 cut(s)	627
BbvI	GCAGC	2 cut(s)	80, 201
BccI	CCATC	3 cut(s)	644, 896, 1245
BceAI	ACGGC	7 cut(s)	364, 616, 682, 799, 1015, 1165, 1285
BciT130I	CCWGG	3 cut(s)	211, 829, 1143
BciVI	GTATCC	1 cut(s)	973
BcnI	CCSGG	4 cut(s)	42, 902, 988, 1000
BcoDI	GTCTC	2 cut(s)	325, 339
BfaI	CTAG	1 cut(s)	326
BfoI	RGCGCY	2 cut(s)	297, 1031
BfuAI	ACCTGC	2 cut(s)	226, 399
BfuI	GTATCC	1 cut(s)	973
BisI	GCNGC	7 cut(s)	94, 190, 193, 271, 297, 522, 732
BlsI	GCNGC	7 cut(s)	95, 191, 194, 272, 298, 523, 733
Bme1390I	CCNGG	7 cut(s)	42, 211, 829, 902, 988, 1000, 1143
Bme18I	GGWCC	2 cut(s)	709, 824
BmeT110I	CYCGRG	1 cut(s)	1199
BmgBI	CACGTC	1 cut(s)	319
BmgT120I	GGNCC	5 cut(s)	126, 127, 645, 709, 824
BmiI	GGNNCC	7 cut(s)	65, 128, 295, 314, 826, 1029, 1256
BmrFI	CCNGG	7 cut(s)	42, 211, 829, 902, 988, 1000, 1143
BmsI	GCATC	2 cut(s)	228, 501
BoxI	GACNNNNGTC	1 cut(s)	1082
BpiI	GAAGAC	1 cut(s)	1115
BpmI	CTGGAG	1 cut(s)	169
Bpu10I	CCTNAGC	1 cut(s)	627
BpuEI	CTTGAG	2 cut(s)	404, 510
BpuMI	CCSGG	4 cut(s)	42, 902, 988, 1000
BsaAI	YACGTR	1 cut(s)	508
BsaHI	GRCGYC	5 cut(s)	38, 294, 351, 534, 1028
BsaJI	CCNNGG	6 cut(s)	210, 546, 827, 828, 877, 901
BsaWI	WCCGGW	4 cut(s)	537, 797, 1035, 1063
BsaXI	ACNNNNNCTCC	2 cut(s)	336, 366
Bsc4I	CCNNNNNNNGG	5 cut(s)	210, 537, 642, 900, 1148
Bse118I	RCCGGY	7 cut(s)	290, 530, 584, 715, 746, 797, 1063
Bse1I	ACTGG	2 cut(s)	82, 223
BseBI	CCWGG	3 cut(s)	211, 829, 1143
BseDI	CCNNGG	6 cut(s)	210, 546, 827, 828, 877, 901
BseGI	GGATG	3 cut(s)	219, 870, 983
BseLI	CCNNNNNNNGG	5 cut(s)	210, 537, 642, 900, 1148
BseMII	CTCAG	1 cut(s)	641
BseNI	ACTGG	2 cut(s)	82, 223
BseRI	GAGGAG	2 cut(s)	407, 461
BseSI	GKGCMC	1 cut(s)	130
BseX3I	CGGCCG	1 cut(s)	1185
BseXI	GCAGC	2 cut(s)	80, 201
Bsh1236I	CGCG	3 cut(s)	152, 342, 1172
Bsh1285I	CGRYCG	1 cut(s)	1188
BshFI	GGCC	8 cut(s)	128, 588, 620, 647, 719, 786, 848, 1187
BshNI	GGYRCC	3 cut(s)	293, 1027, 1254
BshTI	ACCGGT	2 cut(s)	797, 1063
BsiEI	CGRYCG	1 cut(s)	1188
BsiHKCI	CYCGRG	1 cut(s)	1199
BsiWI	CGTACG	1 cut(s)	704
BslFI	GGGAC	1 cut(s)	846
BslI	CCNNNNNNNGG	5 cut(s)	210, 537, 642, 900, 1148
BsmAI	GTCTC	2 cut(s)	325, 339
BsmBI	CGTCTC	2 cut(s)	325, 339
BsmFI	GGGAC	1 cut(s)	846
BsnI	GGCC	8 cut(s)	128, 588, 620, 647, 719, 786, 848, 1187
BsoBI	CYCGRG	1 cut(s)	1199
Bsp120I	GGGCCC	1 cut(s)	126
Bsp1286I	GDGCHC	1 cut(s)	130
Bsp143I	GATC	3 cut(s)	255, 915, 1038
Bsp19I	CCATGG	1 cut(s)	546
BspANI	GGCC	8 cut(s)	128, 588, 620, 647, 719, 786, 848, 1187
BspCNI	CTCAG	1 cut(s)	640
BspFNI	CGCG	3 cut(s)	152, 342, 1172
BspLI	GGNNCC	7 cut(s)	65, 128, 295, 314, 826, 1029, 1256
BspMI	ACCTGC	2 cut(s)	226, 399
BspPI	GGATC	3 cut(s)	250, 923, 1046
BspT107I	GGYRCC	3 cut(s)	293, 1027, 1254
BsrBI	CCGCTC	3 cut(s)	61, 264, 1230
BsrFI	RCCGGY	7 cut(s)	290, 530, 584, 715, 746, 797, 1063
BsrI	ACTGG	2 cut(s)	82, 223
BssAI	RCCGGY	7 cut(s)	290, 530, 584, 715, 746, 797, 1063
BssECI	CCNNGG	6 cut(s)	210, 546, 827, 828, 877, 901
BssMI	GATC	3 cut(s)	255, 915, 1038
BssNI	GRCGYC	5 cut(s)	38, 294, 351, 534, 1028
BssT1I	CCWWGG	2 cut(s)	546, 877
Bst2UI	CCWGG	3 cut(s)	211, 829, 1143
Bst4CI	ACNGT	4 cut(s)	493, 704, 817, 1072
Bst6I	CTCTTC	2 cut(s)	986, 1186
BstACI	GRCGYC	5 cut(s)	38, 294, 351, 534, 1028
BstBAI	YACGTR	1 cut(s)	508
BstC8I	GCNNGC	7 cut(s)	406, 532, 586, 717, 721, 1170, 1286
BstDEI	CTNAG	1 cut(s)	627
BstDSI	CCRYGG	1 cut(s)	546
BstF5I	GGATG	3 cut(s)	219, 870, 983
BstFNI	CGCG	3 cut(s)	152, 342, 1172
BstH2I	RGCGCY	2 cut(s)	297, 1031
BstHHI	GCGC	3 cut(s)	296, 342, 1030
BstKTI	GATC	3 cut(s)	258, 918, 1041
BstMAI	GTCTC	2 cut(s)	325, 339
BstMBI	GATC	3 cut(s)	255, 915, 1038
BstMCI	CGRYCG	1 cut(s)	1188
BstMWI	GCNNNNNNNGC	4 cut(s)	149, 270, 348, 527
BstNI	CCWGG	3 cut(s)	211, 829, 1143
BstNSI	RCATGY	2 cut(s)	277, 1141
BstPAI	GACNNNNGTC	1 cut(s)	1082
BstSCI	CCNGG	7 cut(s)	40, 209, 827, 900, 986, 998, 1141
BstSLI	GKGCMC	1 cut(s)	130
BstUI	CGCG	3 cut(s)	152, 342, 1172
BstV1I	GCAGC	2 cut(s)	80, 201
BstV2I	GAAGAC	1 cut(s)	1115
BstX2I	RGATCY	2 cut(s)	255, 1038
BstYI	RGATCY	2 cut(s)	255, 1038
BstZI	CGGCCG	1 cut(s)	1185
BsuI	GTATCC	1 cut(s)	973
BsuRI	GGCC	8 cut(s)	128, 588, 620, 647, 719, 786, 848, 1187
BtgI	CCRYGG	1 cut(s)	546
BtgZI	GCGATG	1 cut(s)	124
BtrI	CACGTC	1 cut(s)	319
BtsCI	GGATG	3 cut(s)	219, 870, 983
BtsIMutI	CAGTG	2 cut(s)	230, 1077
BveI	ACCTGC	2 cut(s)	226, 399
Cac8I	GCNNGC	7 cut(s)	406, 532, 586, 717, 721, 1170, 1286
CfoI	GCGC	3 cut(s)	296, 342, 1030
Cfr10I	RCCGGY	7 cut(s)	290, 530, 584, 715, 746, 797, 1063
Cfr13I	GGNCC	5 cut(s)	126, 127, 645, 709, 824
CseI	GACGC	4 cut(s)	46, 141, 340, 523
Csp6I	GTAC	7 cut(s)	541, 705, 820, 996, 1033, 1204, 1255
CspAI	ACCGGT	2 cut(s)	797, 1063
CviAII	CATG	6 cut(s)	73, 115, 274, 547, 844, 1138
CviQI	GTAC	7 cut(s)	541, 705, 820, 996, 1033, 1204, 1255
DdeI	CTNAG	1 cut(s)	627
DinI	GGCGCC	2 cut(s)	295, 1029
DpnI	GATC	3 cut(s)	257, 917, 1040
DpnII	GATC	3 cut(s)	255, 915, 1038
DrdI	GACNNNNNNGTC	1 cut(s)	353
DseDI	GACNNNNNNGTC	1 cut(s)	353
EaeI	YGGCCR	3 cut(s)	618, 717, 1185
EagI	CGGCCG	1 cut(s)	1185
Eam1104I	CTCTTC	2 cut(s)	986, 1186
EarI	CTCTTC	2 cut(s)	986, 1186
EciI	GGCGGA	1 cut(s)	193
EclXI	CGGCCG	1 cut(s)	1185
Eco130I	CCWWGG	2 cut(s)	546, 877
Eco24I	GRGCYC	1 cut(s)	130
Eco47I	GGWCC	2 cut(s)	709, 824
Eco52I	CGGCCG	1 cut(s)	1185
Eco72I	CACGTG	1 cut(s)	508
Eco88I	CYCGRG	1 cut(s)	1199
EcoO109I	RGGNCCY	1 cut(s)	127
EcoRII	CCWGG	3 cut(s)	209, 827, 1141
EcoT14I	CCWWGG	2 cut(s)	546, 877
EcoT38I	GRGCYC	1 cut(s)	130
EgeI	GGCGCC	2 cut(s)	295, 1029
EheI	GGCGCC	2 cut(s)	295, 1029
ErhI	CCWWGG	2 cut(s)	546, 877
Esp3I	CGTCTC	2 cut(s)	325, 339
FaeI	CATG	6 cut(s)	76, 118, 277, 550, 847, 1141
FaqI	GGGAC	1 cut(s)	846
FatI	CATG	6 cut(s)	72, 114, 273, 546, 843, 1137
FauI	CCCGC	3 cut(s)	872, 1011, 1223
FblI	GTMKAC	1 cut(s)	663
Fnu4HI	GCNGC	7 cut(s)	94, 190, 193, 271, 297, 522, 732
FokI	GGATG	3 cut(s)	206, 857, 970
FriOI	GRGCYC	1 cut(s)	130
Fsp4HI	GCNGC	7 cut(s)	94, 190, 193, 271, 297, 522, 732
FspBI	CTAG	1 cut(s)	326
GlaI	GCGC	3 cut(s)	295, 341, 1029
GluI	GCNGC	7 cut(s)	94, 190, 193, 271, 297, 522, 732
GsuI	CTGGAG	1 cut(s)	169
HaeII	RGCGCY	2 cut(s)	297, 1031
HaeIII	GGCC	8 cut(s)	128, 588, 620, 647, 719, 786, 848, 1187
HgaI	GACGC	4 cut(s)	46, 141, 340, 523
HhaI	GCGC	3 cut(s)	296, 342, 1030
Hin1I	GRCGYC	5 cut(s)	38, 294, 351, 534, 1028
Hin1II	CATG	6 cut(s)	76, 118, 277, 550, 847, 1141
Hin6I	GCGC	3 cut(s)	294, 340, 1028
HinP1I	GCGC	3 cut(s)	294, 340, 1028
HincII	GTYRAC	1 cut(s)	1087
HindII	GTYRAC	1 cut(s)	1087
HinfI	GANTC	2 cut(s)	182, 303
HphI	GGTGA	8 cut(s)	280, 493, 761, 805, 853, 883, 1053, 1078
Hpy166II	GTNNAC	7 cut(s)	501, 571, 664, 820, 1087, 1135, 1164
Hpy188I	TCNGA	3 cut(s)	171, 359, 694
Hpy188III	TCNNGA	4 cut(s)	310, 421, 602, 955
Hpy8I	GTNNAC	7 cut(s)	501, 571, 664, 820, 1087, 1135, 1164
Hpy99I	CGWCG	8 cut(s)	40, 157, 311, 356, 359, 394, 446, 1157
HpyAV	CCTTC	3 cut(s)	391, 952, 1108
HpyCH4III	ACNGT	4 cut(s)	493, 704, 817, 1072
HpyCH4IV	ACGT	5 cut(s)	318, 507, 567, 681, 1155
HpyCH4V	TGCA	4 cut(s)	514, 612, 700, 1225
HpyF10VI	GCNNNNNNNGC	4 cut(s)	149, 270, 348, 527
HpyF3I	CTNAG	1 cut(s)	627
HpySE526I	ACGT	5 cut(s)	318, 507, 567, 681, 1155
Hsp92I	GRCGYC	5 cut(s)	38, 294, 351, 534, 1028
Hsp92II	CATG	6 cut(s)	76, 118, 277, 550, 847, 1141
HspAI	GCGC	3 cut(s)	294, 340, 1028
KasI	GGCGCC	2 cut(s)	293, 1027
KpnI	GGTACC	1 cut(s)	1258
KroI	GCCGGC	3 cut(s)	530, 584, 715
KroNI	GCCGGC	3 cut(s)	532, 586, 717
Kzo9I	GATC	3 cut(s)	255, 915, 1038
LmnI	GCTCC	4 cut(s)	63, 269, 312, 420
Lsp1109I	GCAGC	2 cut(s)	80, 201
LweI	GCATC	2 cut(s)	228, 501
MaeI	CTAG	1 cut(s)	326
MaeII	ACGT	5 cut(s)	318, 507, 567, 681, 1155
MaeIII	GTNAC	2 cut(s)	592, 1066
MalI	GATC	3 cut(s)	257, 917, 1040
MbiI	CCGCTC	3 cut(s)	61, 264, 1230
MboI	GATC	3 cut(s)	255, 915, 1038
MboII	GAAGA	6 cut(s)	29, 764, 1003, 1024, 1120, 1203
MflI	RGATCY	2 cut(s)	255, 1038
MhlI	GDGCHC	1 cut(s)	130
MluCI	AATT	7 cut(s)	360, 695, 756, 792, 1045, 1219, 1294
Mly113I	GGCGCC	2 cut(s)	294, 1028
MlyI	GAGTC	1 cut(s)	176
MmeI	TCCRAC	1 cut(s)	337
MroNI	GCCGGC	3 cut(s)	530, 584, 715
MseI	TTAA	3 cut(s)	671, 1044, 1297
MslI	CAYNNNNRTG	1 cut(s)	506
MspA1I	CMGCKG	1 cut(s)	93
MspR9I	CCNGG	7 cut(s)	42, 211, 829, 902, 988, 1000, 1143
MvaI	CCWGG	3 cut(s)	211, 829, 1143
MvnI	CGCG	3 cut(s)	152, 342, 1172
MwoI	GCNNNNNNNGC	4 cut(s)	149, 270, 348, 527
NaeI	GCCGGC	3 cut(s)	532, 586, 717
NarI	GGCGCC	2 cut(s)	294, 1028
NciI	CCSGG	4 cut(s)	42, 902, 988, 1000
NcoI	CCATGG	1 cut(s)	546
NdeII	GATC	3 cut(s)	255, 915, 1038
NgoMIV	GCCGGC	3 cut(s)	530, 584, 715
NlaIII	CATG	6 cut(s)	76, 118, 277, 550, 847, 1141
NlaIV	GGNNCC	7 cut(s)	65, 128, 295, 314, 826, 1029, 1256
NmuCI	GTSAC	2 cut(s)	592, 1066
NspI	RCATGY	2 cut(s)	277, 1141
OliI	CACNNNNGTG	1 cut(s)	506
PasI	CCCWGGG	1 cut(s)	828
PcsI	WCGNNNNNNNCGW	4 cut(s)	315, 447, 588, 672
PdiI	GCCGGC	3 cut(s)	532, 586, 717
PfeI	GAWTC	1 cut(s)	303
Pfl23II	CGTACG	1 cut(s)	704
PflFI	GACNNNGTC	2 cut(s)	151, 350
PflMI	CCANNNNNTGG	1 cut(s)	642
PfoI	TCCNGGA	1 cut(s)	986
PinAI	ACCGGT	2 cut(s)	797, 1063
PkrI	GCNGC	7 cut(s)	95, 191, 194, 272, 298, 523, 733
PleI	GAGTC	1 cut(s)	176
PluTI	GGCGCC	2 cut(s)	297, 1031
PmaCI	CACGTG	1 cut(s)	508
PmlI	CACGTG	1 cut(s)	508
PpsI	GAGTC	1 cut(s)	176
Ppu21I	YACGTR	1 cut(s)	508
PshAI	GACNNNNGTC	1 cut(s)	1082
Psp1406I	AACGTT	1 cut(s)	681
Psp6I	CCWGG	3 cut(s)	209, 827, 1141
PspCI	CACGTG	1 cut(s)	508
PspGI	CCWGG	3 cut(s)	209, 827, 1141
PspLI	CGTACG	1 cut(s)	704
PspN4I	GGNNCC	7 cut(s)	65, 128, 295, 314, 826, 1029, 1256
PspOMI	GGGCCC	1 cut(s)	126
PspPI	GGNCC	5 cut(s)	126, 127, 645, 709, 824
PsuI	RGATCY	2 cut(s)	255, 1038
PsyI	GACNNNGTC	2 cut(s)	151, 350
RsaI	GTAC	7 cut(s)	542, 706, 821, 997, 1034, 1205, 1256
RsaNI	GTAC	7 cut(s)	541, 705, 820, 996, 1033, 1204, 1255
RseI	CAYNNNNRTG	1 cut(s)	506
SaqAI	TTAA	3 cut(s)	671, 1044, 1297
SatI	GCNGC	7 cut(s)	94, 190, 193, 271, 297, 522, 732
Sau3AI	GATC	3 cut(s)	255, 915, 1038
Sau96I	GGNCC	5 cut(s)	126, 127, 645, 709, 824
SchI	GAGTC	1 cut(s)	176
ScrFI	CCNGG	7 cut(s)	42, 211, 829, 902, 988, 1000, 1143
SduI	GDGCHC	1 cut(s)	130
SfaNI	GCATC	2 cut(s)	228, 501
SfoI	GGCGCC	2 cut(s)	295, 1029
SgrAI	CRCCGGYG	2 cut(s)	290, 1063
SinI	GGWCC	2 cut(s)	709, 824
SmiMI	CAYNNNNRTG	1 cut(s)	506
SmlI	CTYRAG	2 cut(s)	419, 525
SmoI	CTYRAG	2 cut(s)	419, 525
Sse9I	AATT	7 cut(s)	360, 695, 756, 792, 1045, 1219, 1294
SspDI	GGCGCC	2 cut(s)	293, 1027
SspMI	CTAG	1 cut(s)	326
StyD4I	CCNGG	7 cut(s)	40, 209, 827, 900, 986, 998, 1141
StyI	CCWWGG	2 cut(s)	546, 877
TaaI	ACNGT	4 cut(s)	493, 704, 817, 1072
TaiI	ACGT	5 cut(s)	321, 510, 570, 684, 1158
TaqI	TCGA	7 cut(s)	35, 57, 104, 444, 474, 486, 918
TasI	AATT	7 cut(s)	360, 695, 756, 792, 1045, 1219, 1294
TauI	GCSGC	5 cut(s)	195, 273, 299, 524, 734
TfiI	GAWTC	1 cut(s)	303
Tru1I	TTAA	3 cut(s)	671, 1044, 1297
Tru9I	TTAA	3 cut(s)	671, 1044, 1297
TscAI	CASTG	2 cut(s)	230, 1077
TseFI	GTSAC	2 cut(s)	592, 1066
TseI	GCWGC	2 cut(s)	93, 189
Tsp45I	GTSAC	2 cut(s)	592, 1066
TspDTI	ATGAA	3 cut(s)	89, 166, 427
TspGWI	ACGGA	5 cut(s)	268, 675, 722, 837, 1149
TspRI	CASTG	2 cut(s)	230, 1077
Tth111I	GACNNNGTC	2 cut(s)	151, 350
Van91I	CCANNNNNTGG	1 cut(s)	642
VpaK11BI	GGWCC	2 cut(s)	709, 824
XceI	RCATGY	2 cut(s)	277, 1141
XmiI	GTMKAC	1 cut(s)	663
XspI	CTAG	1 cut(s)	326

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.