MD14G1051500.v1.1

protein ROOT PRIMORDIUM DEFECTIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0014471
Physical Location & Seq
Reverse (-)
4937298 .. 4939931
2634 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1051500.v1.1.491

Sequence Viewer

Length: 1365 bp
ATGAAGGCTTTCATCTTCTCTCTCCTAAAACCCCAAACCCCCTCCTCCTCACCCACCAACCTACTCCTCCTCCTCCACCGCCACCACCAACGGCGGGGCATAACCAAAGTCCGCCTCAAATGGGTCAAAAACCGAAGCATCGACCACGTCATCGAAACCGAGACTGACCTCAAAGCCGCCTGCCTCCTCAAGGACGCAATCAAGCGCTCCCCCACCGGCTTCCTCACCGCCAAGTCCTTCGCCGACTGGCAGAAGCTCCTCGGCCTCACCGTCCCCGTCCTCCGCTTCCTCCGCAGGTATCCAACTCTTTTCCAAGAATTCCCACATGCCCGTTACGCCAATTTGCCATGTTTCAAATTAACAGACACAGCATTGTTGTTGGATTCACAAGAACAAAGTATACACAAAACCCATGAGAGTGACACTGTGGAGAGGCTCTGTAGAGTGCTTATGATGACAAAGTCTAAGACTTTACCTCTCCAATCGTTGTATCCTTTGAAATGGGATCTGGGTTTGCCTGATGGTTTCGAAAAAGTGTTGGTTCCGAAATACCCTGATTGTTTTCGGATTGTTAAGGCCTCGAATGGGGTCGGATGCTTGAAGGCCATACAATGGCCTAAAGAGTATGCGGTTTCGGAATTGCAGAGGAGTAGAGAGGTTGGTGATTTAGGGGATGAGTATAGGCAGTTCAAAAGGGGGCAGACGCTGTTGGCATTTCCGATGAATTTTCCAAGGGGTTATGGTGGGCAGAAGAAGGTTAGGGCATGGATGGAGGAGTTCCAGAAGTTACCGTATATTTCACCATATGAGGATTCTAGGCATATTGATCCTAAGAGTGATCTTATGGAGAAAAGGGTAGTTGGAGTTTTGCATGAGTTCTTGAGCTTGACTCTTCATAAGAAGACTAAGAGGAACTACTTGAGAAGCTTGAGGGAGGAATTGAATCTTCCACATAAGTTTACGAGAATCTTCACAAGGTATCCGGGTATTTTCTACCTCTCATTGAAGTGTAAAACAACCACTGTTTCACTCAGGGAAGGGTACCATAGAGGGAAGTTAGTGAACCCACATCCCCTCACAAGTTTGAGAGAGAAGTTCTATTACGTCATGAGAACTGGGCTTCTTTATCGGGGTAAAGGTGTGAACATGCTATCCCCGGAAGAGATTTTGCTTAACAGTATGGAGAGTGAAAGTGGGCAGGAAGAATCCGAAGAGGAAGAGACTGGAACAGGTGATGAATGCCATGAAGATGAACCTTTCTTCAAGTGGTGCAGCATTTCAAGTGTAATACGAAGGCGATGTTTCGAGGATGCAGGAAGAACCTTCGAGGTGAAATCATCAAACTCTTCCCAACAGTGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PORR

Protein Analysis

455

Amino Acids

52.85

Weight (kDa)

9.43

Isoelectric Point (pI)

56.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PORR PF11955 42 - 373 6.8e-120 Plant organelle RNA recognition domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014471)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58520 AT3G58520
fragaria_vesca FvH4_6g21580
malus_domestica MD14G1051500.v1.1
prunus_persica Prupe.7G084500_v2.0.a1
pyrus_communis pycom14g04280
rosa_chinensis RchiOBHm_Chr3g0478831
rosa_laevigata RLG00000023622
rosa_multiflora Rmu_sc0004831.1_g000011 Rmu_sc0005751.1_g000005 Rmu_sc0037242.1_g000002
rosa_roxburghii Rroxscaffold_6G00403040
rosa_rugosa Rorug03G0168500
rosa_samantha Rh3BG252100 Rh3CG249000 Rh3DG245200
rosa_wichuraiana Rw3G019800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 285
Acc65I GGTACC 1 cut(s) 1041
AccB1I GGYRCC 1 cut(s) 1041
AccB7I CCANNNNNTGG 1 cut(s) 612
AccI GTMKAC 1 cut(s) 400
AciI CCGC 8 cut(s) 79, 94, 112, 177, 228, 283, 292, 629
AclWI GGATC 2 cut(s) 513, 821
AcsI RAATTY 2 cut(s) 317, 724
AfaI GTAC 1 cut(s) 1043
AfeI AGCGCT 1 cut(s) 206
AfiI CCNNNNNNNGG 6 cut(s) 94, 121, 190, 585, 612, 1356
AgsI TTSAA 8 cut(s) 355, 499, 601, 691, 943, 1006, 1264, 1281
AjiI CACGTC 1 cut(s) 148
AloI GAACNNNNNNTCC 2 cut(s) 1136, 1168
AluBI AGCT 3 cut(s) 256, 885, 927
AluI AGCT 3 cut(s) 256, 885, 927
Alw26I GTCTC 2 cut(s) 155, 1214
AlwI GGATC 2 cut(s) 513, 821
AlwNI CAGNNNCTG 1 cut(s) 706
Aor51HI AGCGCT 1 cut(s) 206
AoxI GGCC 4 cut(s) 262, 576, 603, 614
ApeKI GCWGC 1 cut(s) 1272
ApoI RAATTY 2 cut(s) 317, 724
Asp700I GAANNNNTTC 1 cut(s) 8
Asp718I GGTACC 1 cut(s) 1041
AspLEI GCGC 1 cut(s) 207
AsuC2I CCSGG 2 cut(s) 984, 1157
AsuHPI GGTGA 7 cut(s) 42, 217, 259, 674, 792, 1244, 1342
AsuII TTCGAA 1 cut(s) 528
BanI GGYRCC 1 cut(s) 1041
BarI GAAGNNNNNNTAC 2 cut(s) 1085, 1117
BbsI GAAGAC 1 cut(s) 908
BbvI GCAGC 1 cut(s) 1284
BccI CCATC 2 cut(s) 515, 763
BceAI ACGGC 1 cut(s) 107
BciVI GTATCC 3 cut(s) 309, 501, 990
BcnI CCSGG 2 cut(s) 984, 1157
BcoDI GTCTC 2 cut(s) 155, 1214
BfaI CTAG 1 cut(s) 816
BfmI CTRYAG 1 cut(s) 439
BfoI RGCGCY 1 cut(s) 208
BfuAI ACCTGC 1 cut(s) 285
BfuI GTATCC 3 cut(s) 309, 501, 990
BisI GCNGC 2 cut(s) 177, 1273
BlsI GCNGC 2 cut(s) 178, 1274
Bme1390I CCNGG 2 cut(s) 984, 1157
BmgBI CACGTC 1 cut(s) 148
BmiI GGNNCC 2 cut(s) 543, 1043
BmrFI CCNGG 2 cut(s) 984, 1157
BmrI ACTGGG 1 cut(s) 1125
BmsI GCATC 3 cut(s) 147, 584, 1300
BmuI ACTGGG 1 cut(s) 1125
BpiI GAAGAC 1 cut(s) 908
BplI GAGNNNNNCTC 2 cut(s) 874, 906
Bpu14I TTCGAA 1 cut(s) 528
BpuEI CTTGAG 4 cut(s) 173, 901, 940, 949
BpuMI CCSGG 2 cut(s) 984, 1157
BsaJI CCNNGG 3 cut(s) 259, 731, 1155
BsaXI ACNNNNNCTCC 2 cut(s) 54, 84
Bsc4I CCNNNNNNNGG 6 cut(s) 94, 121, 190, 585, 612, 1356
Bse118I RCCGGY 1 cut(s) 215
Bse1I ACTGG 3 cut(s) 251, 1120, 1228
BseDI CCNNGG 3 cut(s) 259, 731, 1155
BseGI GGATG 5 cut(s) 599, 679, 774, 1069, 1315
BseLI CCNNNNNNNGG 6 cut(s) 94, 121, 190, 585, 612, 1356
BseMII CTCAG 1 cut(s) 1045
BseNI ACTGG 3 cut(s) 251, 1120, 1228
BseRI GAGGAG 9 cut(s) 34, 37, 56, 59, 62, 176, 248, 661, 788
BseXI GCAGC 1 cut(s) 1284
BsgI GTGCAG 1 cut(s) 1291
BshFI GGCC 4 cut(s) 264, 578, 605, 616
BshNI GGYRCC 1 cut(s) 1041
BsiSI CCGG 3 cut(s) 216, 983, 1157
BslFI GGGAC 1 cut(s) 257
BslI CCNNNNNNNGG 6 cut(s) 94, 121, 190, 585, 612, 1356
BsmAI GTCTC 2 cut(s) 155, 1214
BsmFI GGGAC 1 cut(s) 257
BsmI GAATGC 1 cut(s) 1244
BsnI GGCC 4 cut(s) 264, 578, 605, 616
Bsp119I TTCGAA 1 cut(s) 528
Bsp143I GATC 3 cut(s) 505, 826, 838
BspACI CCGC 8 cut(s) 79, 94, 112, 177, 228, 283, 292, 629
BspANI GGCC 4 cut(s) 264, 578, 605, 616
BspCNI CTCAG 1 cut(s) 1044
BspHI TCATGA 1 cut(s) 1107
BspLI GGNNCC 2 cut(s) 543, 1043
BspMI ACCTGC 1 cut(s) 285
BspPI GGATC 2 cut(s) 513, 821
BspT104I TTCGAA 1 cut(s) 528
BspT107I GGYRCC 1 cut(s) 1041
BsrFI RCCGGY 1 cut(s) 215
BsrI ACTGG 3 cut(s) 251, 1120, 1228
BssAI RCCGGY 1 cut(s) 215
BssECI CCNNGG 3 cut(s) 259, 731, 1155
BssMI GATC 3 cut(s) 505, 826, 838
BssNAI GTATAC 1 cut(s) 401
BssT1I CCWWGG 1 cut(s) 731
Bst1107I GTATAC 1 cut(s) 401
Bst4CI ACNGT 6 cut(s) 271, 427, 792, 1024, 1178, 1356
Bst6I CTCTTC 5 cut(s) 897, 1155, 1206, 1212, 1351
BstBI TTCGAA 1 cut(s) 528
BstC8I GCNNGC 1 cut(s) 181
BstDEI CTNAG 4 cut(s) 465, 831, 906, 1031
BstENI CCTNNNNNAGG 1 cut(s) 188
BstF5I GGATG 5 cut(s) 599, 679, 774, 1069, 1315
BstH2I RGCGCY 1 cut(s) 208
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 3 cut(s) 508, 829, 841
BstMAI GTCTC 2 cut(s) 155, 1214
BstMBI GATC 3 cut(s) 505, 826, 838
BstMWI GCNNNNNNNGC 2 cut(s) 291, 335
BstNSI RCATGY 2 cut(s) 329, 1150
BstSCI CCNGG 2 cut(s) 982, 1155
BstSFI CTRYAG 1 cut(s) 439
BstV1I GCAGC 1 cut(s) 1284
BstV2I GAAGAC 1 cut(s) 908
BstX2I RGATCY 1 cut(s) 505
BstYI RGATCY 1 cut(s) 505
BstZ17I GTATAC 1 cut(s) 401
BsuI GTATCC 3 cut(s) 309, 501, 990
BsuRI GGCC 4 cut(s) 264, 578, 605, 616
BtgZI GCGATG 1 cut(s) 1312
BtrI CACGTC 1 cut(s) 148
BtsCI GGATG 5 cut(s) 599, 679, 774, 1069, 1315
BtsIMutI CAGTG 3 cut(s) 423, 1020, 1361
BveI ACCTGC 1 cut(s) 285
Cac8I GCNNGC 1 cut(s) 181
CaiI CAGNNNCTG 1 cut(s) 706
CciI TCATGA 1 cut(s) 1107
CfoI GCGC 1 cut(s) 207
Cfr10I RCCGGY 1 cut(s) 215
CseI GACGC 2 cut(s) 203, 712
Csp6I GTAC 1 cut(s) 1042
CviAII CATG 8 cut(s) 326, 348, 413, 765, 872, 1108, 1147, 1244
CviQI GTAC 1 cut(s) 1042
DdeI CTNAG 4 cut(s) 465, 831, 906, 1031
DpnI GATC 3 cut(s) 507, 828, 840
DpnII GATC 3 cut(s) 505, 826, 838
Eam1104I CTCTTC 5 cut(s) 897, 1155, 1206, 1212, 1351
EarI CTCTTC 5 cut(s) 897, 1155, 1206, 1212, 1351
EciI GGCGGA 1 cut(s) 101
Eco130I CCWWGG 1 cut(s) 731
Eco147I AGGCCT 1 cut(s) 578
Eco47III AGCGCT 1 cut(s) 206
EcoNI CCTNNNNNAGG 1 cut(s) 188
EcoRI GAATTC 1 cut(s) 317
EcoT14I CCWWGG 1 cut(s) 731
ErhI CCWWGG 1 cut(s) 731
FaeI CATG 8 cut(s) 329, 351, 416, 768, 875, 1111, 1150, 1247
FaqI GGGAC 1 cut(s) 257
FatI CATG 8 cut(s) 325, 347, 412, 764, 871, 1107, 1146, 1243
FauI CCCGC 1 cut(s) 87
FauNDI CATATG 1 cut(s) 805
FblI GTMKAC 1 cut(s) 400
Fnu4HI GCNGC 2 cut(s) 177, 1273
FokI GGATG 5 cut(s) 606, 686, 781, 1056, 1322
Fsp4HI GCNGC 2 cut(s) 177, 1273
FspBI CTAG 1 cut(s) 816
GlaI GCGC 1 cut(s) 206
GluI GCNGC 2 cut(s) 177, 1273
HaeII RGCGCY 1 cut(s) 208
HaeIII GGCC 4 cut(s) 264, 578, 605, 616
HapII CCGG 3 cut(s) 216, 983, 1157
HgaI GACGC 2 cut(s) 203, 712
HhaI GCGC 1 cut(s) 207
Hin1II CATG 8 cut(s) 329, 351, 416, 768, 875, 1111, 1150, 1247
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HindIII AAGCTT 1 cut(s) 925
HinfI GANTC 6 cut(s) 383, 812, 889, 943, 966, 1205
HpaII CCGG 3 cut(s) 216, 983, 1157
HphI GGTGA 7 cut(s) 42, 217, 259, 674, 792, 1244, 1342
Hpy166II GTNNAC 4 cut(s) 401, 960, 1063, 1144
Hpy188I TCNGA 6 cut(s) 546, 567, 593, 637, 720, 1210
Hpy188III TCNNGA 3 cut(s) 781, 880, 1108
Hpy8I GTNNAC 4 cut(s) 401, 960, 1063, 1144
HpyAV CCTTC 6 cut(s) 247, 595, 748, 1031, 1287, 1333
HpyCH4III ACNGT 6 cut(s) 271, 427, 792, 1024, 1178, 1356
HpyCH4IV ACGT 2 cut(s) 147, 1104
HpyCH4V TGCA 4 cut(s) 643, 871, 1272, 1313
HpyF10VI GCNNNNNNNGC 2 cut(s) 291, 335
HpyF3I CTNAG 4 cut(s) 465, 831, 906, 1031
HpySE526I ACGT 2 cut(s) 147, 1104
Hsp92II CATG 8 cut(s) 329, 351, 416, 768, 875, 1111, 1150, 1247
HspAI GCGC 1 cut(s) 205
KpnI GGTACC 1 cut(s) 1045
Kzo9I GATC 3 cut(s) 505, 826, 838
LmnI GCTCC 2 cut(s) 212, 261
Lsp1109I GCAGC 1 cut(s) 1284
LweI GCATC 3 cut(s) 147, 584, 1300
MaeI CTAG 1 cut(s) 816
MaeII ACGT 2 cut(s) 147, 1104
MaeIII GTNAC 3 cut(s) 332, 419, 786
MalI GATC 3 cut(s) 507, 828, 840
MboI GATC 3 cut(s) 505, 826, 838
MflI RGATCY 1 cut(s) 505
MluCI AATT 6 cut(s) 317, 340, 356, 638, 724, 938
MlyI GAGTC 1 cut(s) 883
MmeI TCCRAC 4 cut(s) 326, 360, 571, 841
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 3 cut(s) 359, 573, 1173
MslI CAYNNNNRTG 3 cut(s) 417, 1006, 1248
MspI CCGG 3 cut(s) 216, 983, 1157
MspR9I CCNGG 2 cut(s) 984, 1157
Mva1269I GAATGC 1 cut(s) 1244
MwoI GCNNNNNNNGC 2 cut(s) 291, 335
NciI CCSGG 2 cut(s) 984, 1157
NdeI CATATG 1 cut(s) 805
NdeII GATC 3 cut(s) 505, 826, 838
NlaIII CATG 8 cut(s) 329, 351, 416, 768, 875, 1111, 1150, 1247
NlaIV GGNNCC 2 cut(s) 543, 1043
NmeAIII GCCGAG 1 cut(s) 240
NmuCI GTSAC 1 cut(s) 419
NspI RCATGY 2 cut(s) 329, 1150
NspV TTCGAA 1 cut(s) 528
PagI TCATGA 1 cut(s) 1107
PceI AGGCCT 1 cut(s) 578
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PctI GAATGC 1 cut(s) 1244
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 5 cut(s) 383, 812, 943, 966, 1205
PflFI GACNNNGTC 2 cut(s) 146, 460
PflMI CCANNNNNTGG 1 cut(s) 612
PkrI GCNGC 2 cut(s) 178, 1274
PleI GAGTC 1 cut(s) 883
PpsI GAGTC 1 cut(s) 883
PspN4I GGNNCC 2 cut(s) 543, 1043
PsrI GAACNNNNNNTAC 4 cut(s) 384, 416, 671, 703
PstNI CAGNNNCTG 1 cut(s) 706
PsuI RGATCY 1 cut(s) 505
PsyI GACNNNGTC 2 cut(s) 146, 460
RsaI GTAC 1 cut(s) 1043
RsaNI GTAC 1 cut(s) 1042
RseI CAYNNNNRTG 3 cut(s) 417, 1006, 1248
SaqAI TTAA 3 cut(s) 359, 573, 1173
SatI GCNGC 2 cut(s) 177, 1273
Sau3AI GATC 3 cut(s) 505, 826, 838
SchI GAGTC 1 cut(s) 883
ScrFI CCNGG 2 cut(s) 984, 1157
SfaNI GCATC 3 cut(s) 147, 584, 1300
SfcI CTRYAG 1 cut(s) 439
SfuI TTCGAA 1 cut(s) 528
SmiMI CAYNNNNRTG 3 cut(s) 417, 1006, 1248
SmlI CTYRAG 4 cut(s) 188, 880, 919, 928
SmoI CTYRAG 4 cut(s) 188, 880, 919, 928
Sse9I AATT 6 cut(s) 317, 340, 356, 638, 724, 938
SseBI AGGCCT 1 cut(s) 578
SsiI CCGC 8 cut(s) 79, 94, 112, 177, 228, 283, 292, 629
SspMI CTAG 1 cut(s) 816
StuI AGGCCT 1 cut(s) 578
StyD4I CCNGG 2 cut(s) 982, 1155
StyI CCWWGG 1 cut(s) 731
TaaI ACNGT 6 cut(s) 271, 427, 792, 1024, 1178, 1356
TaiI ACGT 2 cut(s) 150, 1107
TaqI TCGA 6 cut(s) 141, 153, 528, 581, 1305, 1326
TasI AATT 6 cut(s) 317, 340, 356, 638, 724, 938
TauI GCSGC 1 cut(s) 179
TfiI GAWTC 5 cut(s) 383, 812, 943, 966, 1205
Tru1I TTAA 3 cut(s) 359, 573, 1173
Tru9I TTAA 3 cut(s) 359, 573, 1173
TscAI CASTG 3 cut(s) 430, 1027, 1361
TseFI GTSAC 1 cut(s) 419
TseI GCWGC 1 cut(s) 1272
Tsp45I GTSAC 1 cut(s) 419
TspDTI ATGAA 6 cut(s) 17, 737, 884, 1251, 1260, 1266
TspRI CASTG 3 cut(s) 430, 1027, 1361
Tth111I GACNNNGTC 2 cut(s) 146, 460
Van91I CCANNNNNTGG 1 cut(s) 612
XagI CCTNNNNNAGG 1 cut(s) 188
XapI RAATTY 2 cut(s) 317, 724
XceI RCATGY 2 cut(s) 329, 1150
XmiI GTMKAC 1 cut(s) 400
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 1 cut(s) 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.