Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0008482

Physical Location & Seq

Genomic Coordinates Reverse (-)

6730729 .. 6732681

Gene Structure 1953 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1063500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1953 bp

ATGATGCTTTTTTTGCTGCAAATTGCTCTACTTCTGGTTCCTGAAGTGGTGCACTGTCTCAACCAAGATGGACTCTCCCTCCTCGCACTCAAATCAGCAATCGAAACCGACCCGACACGGATCCTCGACTCCTGGTCGGAATCCGATCCCACCCCCTGCCACTGGCACGGCGTCGCCTGCACCGGCAACCGAGTCACCGACCTCCTCCTCACCAACAAACGCCTCACTGGCTACATTCCCTCCGAACTTGGTCACCTTGACTCGCTCAAGCGACTCAATCTTTCAAACAACAACTTCTCCATGCCCATGCCGGCCCACCTCTTCAACGCCGACGCCCTCATTTCTCTCGACCTCTCCAACAACTCTCTCGTGGGTCCAATCCCGGACCAAATCCGATCACTCAAGGCACTGAACCACCTCGACCTGTCTTCCAATCTCCTCAACGGCTCCCTCCCCGAGTCTCTCGCCGAGCTTCCCTCCCTCGCTGGAACCCTGAACCTCTCGTACAACAAATTCTCCGGCGGAGTTCCGGCTTCGTACGGGCGGATTCCGGTGCTCGTGAGCCTGGACCTCCGGCACAACAACCTCACCGGAAAAGTGCCTCAGGTGGGATCGCTGGCGAACCAGGGCCCCACCGCGTTTTCCGAAAACCCTAGCCTCTGCGGGATTCCGTTGGAAATTCCGTGCCCGGAAGCCCAAAACCCTGATTCCCCGAAAAGCGGAGGGGAGGATGTTCAAAAGCCTCTCAACCCGGATCCGAGTTTGGTACGGAGGGCAGAGCAGCGAGAAAATGGGAGCGGCGTGTCAGTGACGGTTCCGATAATTTCGGGCGTTTCGGTGGTGGTTGGGGCAGTGTCGGTATCGGTGTGGCTGCGTCGCGTAAAAAGCAGGGCGAAGGAGGGAAAAACGGGAAAAGAGAAAGTGGTAAAGGAGGTGGCTGTCGTGGTGGAGGATGAGGGGGAAGGGCAAAATGGTACATTCGTGGTGGTGGACGAGGGGTTCGAGTTGGAATTGGAGGATTTGCTGAGGGCATCGGCGTACGTGGTGGGGAAGAGCAGGAGTGGGATTACGTACAGAGTGGTCGCCGGGAATAGCGGGAAGGGATCTGGTGCGGCGCCGTCGGTTGTGGCCGTGAGAAGGCTGAGCGAGGGTGACGCCGCGTGGCGGTTCAAGGAGTTTGAGGCTGAGGCGGAGGCGATGGGGAAGGTGGTCCACCCCAATATCGTCCGCCTGAGAGCATATTACTATGCGAATGATGAGAAACTTCTGGTTACTGATTTCATTCGCAATGGCAGCTTGTACAATGCACTCCATGGTAAATTAACCAACTCTTTGCCACCATTGCCATGGACGGCAAGGTTAAAAATTGCTCAGGGGACCGCAAGGGGTTTGGTGTACATACATGAACACAGCCCTCGAAAGTATGTTCATGGAAGCATTAAGTCGACAAAGATCCTTCTCAACGATGATCTCCAACCTTACATATCCGGGGTCGGATTGGCACGTCTTATGTTGGGTACCTCAAAGTTCACCACTTCAGCGTCGAGAAAGCACAACTCGAATCAATCCATTGTGGCTTCTGGTATGGCTGGTCCAAGTTCTTCAACCATTTACTTGGCGCCCGAGGCCCGAGTTTCTGGCAGCAAGTTCACTCAGAAATGTGATGTGTACTCCTTTGGAATTGTGCTCTTGGAGATCTTGACTGGAAGGTTGCCAGATGAGGGGCTCGAAAATGGTGGCGAGGGACTCGAGAGTCTGGTCAGGAAGACATTCAGAGATGAGCGCCCCTTGTCTGAGATCATAGACCCTGTACTTGTGCAAGAAGTTTATGCAAAGAAGCAAGTTGTTGATGCCTTTCACATTGCCCTTAATTGCACTGAGCTCGATCCCGAACTGCGTCCAAGGATGAAAACTGTCTCCGAGAGTCTTGATCGCATCAGACTGCAATGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

651

Amino Acids

70.39

Weight (kDa)

6.72

Isoelectric Point (pI)

36.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	20 - 61	2.5e-11	Leucine rich repeat N-terminal domain
PK_Tyr_Ser-Thr	PF07714	360 - 643	1.2e-26	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	375 - 637	3.5e-20	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0008482)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42290 AT3G57830 AT3G57830 AT3G57830
fragaria_vesca	FvH4_6g22890 FvH4_6g22890 FvH4_6g22890 FvH4_6g22890
malus_domestica	MD12G1062900.v1.1 MD14G1063500.v1.1
prunus_persica	Prupe.7G070700_v2.0.a1
pyrus_communis	pycom12g05540 pycom14g05130
rosa_chinensis	RchiOBHm_Chr3g0480651
rosa_laevigata	RLG00000023462
rosa_multiflora	Rmu_sc0006353.1_g000007
rosa_roxburghii	Rroxscaffold_6G00401170
rosa_rugosa	Rorug03G0184500
rosa_samantha	Rh3AG236800 Rh3BG269800 Rh3CG266300 Rh3DG262300
rosa_wichuraiana	Rw3G021210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1752
Acc65I	GGTACC	1 cut(s)	1517
AccB1I	GGYRCC	3 cut(s)	1114, 1517, 1618
AccBSI	CCGCTC	1 cut(s)	798
AccI	GTMKAC	1 cut(s)	1445
AccII	CGCG	3 cut(s)	638, 879, 1160
AclWI	GGATC	9 cut(s)	115, 128, 140, 619, 749, 762, 1111, 1447, 1880
AcoI	YGGCCR	1 cut(s)	1128
AcsI	RAATTY	2 cut(s)	512, 678
AcuI	CTGAAG	2 cut(s)	63, 1521
AcyI	GRCGYC	5 cut(s)	171, 333, 1115, 1155, 1619
AfiI	CCNNNNNNNGG	6 cut(s)	162, 608, 719, 1137, 1164, 1721
AgsI	TTSAA	5 cut(s)	285, 325, 737, 1171, 1605
AhdI	GACNNNNNGTC	1 cut(s)	133
AjiI	CACGTC	1 cut(s)	1505
AjnI	CCWGG	3 cut(s)	131, 564, 624
AloI	GAACNNNNNNTCC	2 cut(s)	983, 1015
AluBI	AGCT	3 cut(s)	472, 1296, 1882
AluI	AGCT	3 cut(s)	472, 1296, 1882
Alw21I	GWGCWC	4 cut(s)	54, 558, 1689, 1884
Alw26I	GTCTC	3 cut(s)	62, 465, 1921
Alw44I	GTGCAC	1 cut(s)	50
AlwI	GGATC	9 cut(s)	115, 128, 140, 619, 749, 762, 1111, 1447, 1880
Ama87I	CYCGRG	4 cut(s)	455, 1622, 1629, 1748
AoxI	GGCC	4 cut(s)	312, 628, 1128, 1626
ApaI	GGGCCC	1 cut(s)	632
ApaLI	GTGCAC	1 cut(s)	50
ApeKI	GCWGC	5 cut(s)	16, 781, 871, 1293, 1641
ApoI	RAATTY	2 cut(s)	512, 678
ArsI	GACNNNNNNTTYG	2 cut(s)	983, 1015
Asp700I	GAANNNNTTC	1 cut(s)	1769
Asp718I	GGTACC	1 cut(s)	1517
AspLEI	GCGC	3 cut(s)	1117, 1621, 1785
AsuC2I	CCSGG	5 cut(s)	383, 689, 752, 1087, 1489
AsuHPI	GGTGA	6 cut(s)	187, 202, 245, 580, 1163, 1522
AvaI	CYCGRG	4 cut(s)	455, 1622, 1629, 1748
AvaII	GGWCC	6 cut(s)	374, 385, 568, 1210, 1377, 1592
AxyI	CCTNAGG	1 cut(s)	603
BaeGI	GKGCMC	3 cut(s)	54, 632, 689
BaeI	ACNNNNGTAYC	2 cut(s)	966, 999
BamHI	GGATCC	2 cut(s)	120, 754
BanI	GGYRCC	3 cut(s)	1114, 1517, 1618
BanII	GRGCYC	3 cut(s)	632, 1728, 1884
BauI	CACGAG	2 cut(s)	368, 557
BbsI	GAAGAC	2 cut(s)	420, 1772
Bbv12I	GWGCWC	4 cut(s)	54, 558, 1689, 1884
BbvCI	CCTCAGC	2 cut(s)	1025, 1185
BbvI	GCAGC	5 cut(s)	3, 793, 858, 1305, 1653
BccI	CCATC	2 cut(s)	62, 1192
BceAI	ACGGC	5 cut(s)	184, 460, 1102, 1115, 1368
BcgI	CGANNNNNNTGC	2 cut(s)	1864, 1898
BciT130I	CCWGG	3 cut(s)	133, 566, 626
BcnI	CCSGG	5 cut(s)	383, 689, 752, 1087, 1489
BcoDI	GTCTC	3 cut(s)	62, 465, 1921
BfaI	CTAG	1 cut(s)	654
BfoI	RGCGCY	3 cut(s)	1118, 1622, 1786
BglI	GCCNNNNNGGC	1 cut(s)	228
BglII	AGATCT	1 cut(s)	1695
BisI	GCNGC	8 cut(s)	17, 782, 799, 872, 1113, 1158, 1294, 1642
BlpI	GCTNAGC	1 cut(s)	1142
BlsI	GCNGC	8 cut(s)	18, 783, 800, 873, 1114, 1159, 1295, 1643
Bme1390I	CCNGG	8 cut(s)	133, 383, 566, 626, 689, 752, 1087, 1489
Bme18I	GGWCC	6 cut(s)	374, 385, 568, 1210, 1377, 1592
BmeRI	GACNNNNNGTC	1 cut(s)	133
BmeT110I	CYCGRG	4 cut(s)	455, 1622, 1629, 1748
BmgBI	CACGTC	1 cut(s)	1505
BmrFI	CCNGG	8 cut(s)	133, 383, 566, 626, 689, 752, 1087, 1489
BmsI	GCATC	3 cut(s)	1040, 1840, 1944
BpiI	GAAGAC	2 cut(s)	420, 1772
BplI	GAGNNNNNCTC	2 cut(s)	461, 493
Bpu10I	CCTNAGC	3 cut(s)	1025, 1185, 1371
Bpu1102I	GCTNAGC	1 cut(s)	1142
BpuEI	CTTGAG	2 cut(s)	251, 386
BpuMI	CCSGG	5 cut(s)	383, 689, 752, 1087, 1489
BsaAI	YACGTR	2 cut(s)	1042, 1071
BsaHI	GRCGYC	5 cut(s)	171, 333, 1115, 1155, 1619
BsaJI	CCNNGG	6 cut(s)	625, 1312, 1346, 1488, 1623, 1901
BsaWI	WCCGGW	2 cut(s)	550, 590
BsaXI	ACNNNNNCTCC	8 cut(s)	63, 93, 224, 254, 281, 311, 500, 530
Bsc4I	CCNNNNNNNGG	6 cut(s)	162, 608, 719, 1137, 1164, 1721
Bse118I	RCCGGY	2 cut(s)	182, 310
Bse1I	ACTGG	3 cut(s)	167, 232, 1708
Bse21I	CCTNAGG	1 cut(s)	603
Bse3DI	GCAATG	4 cut(s)	1294, 1340, 1860, 1952
BseBI	CCWGG	3 cut(s)	133, 566, 626
BseDI	CCNNGG	6 cut(s)	625, 1312, 1346, 1488, 1623, 1901
BseGI	GGATG	3 cut(s)	736, 958, 1911
BseLI	CCNNNNNNNGG	6 cut(s)	162, 608, 719, 1137, 1164, 1721
BseMI	GCAATG	4 cut(s)	1294, 1340, 1860, 1952
BseMII	CTCAG	9 cut(s)	617, 1016, 1133, 1176, 1223, 1385, 1667, 1785, 1869
BseNI	ACTGG	3 cut(s)	167, 232, 1708
BseRI	GAGGAG	4 cut(s)	71, 194, 197, 428
BseSI	GKGCMC	3 cut(s)	54, 632, 689
BseXI	GCAGC	5 cut(s)	3, 793, 858, 1305, 1653
BsgI	GTGCAG	1 cut(s)	163
Bsh1236I	CGCG	3 cut(s)	638, 879, 1160
BshFI	GGCC	4 cut(s)	314, 630, 1130, 1628
BshNI	GGYRCC	3 cut(s)	1114, 1517, 1618
BsiHKAI	GWGCWC	4 cut(s)	54, 558, 1689, 1884
BsiHKCI	CYCGRG	4 cut(s)	455, 1622, 1629, 1748
BsiWI	CGTACG	2 cut(s)	537, 1038
BslFI	GGGAC	2 cut(s)	1390, 1758
BslI	CCNNNNNNNGG	6 cut(s)	162, 608, 719, 1137, 1164, 1721
BsmAI	GTCTC	3 cut(s)	62, 465, 1921
BsmFI	GGGAC	2 cut(s)	1390, 1758
BsnI	GGCC	4 cut(s)	314, 630, 1130, 1628
BsoBI	CYCGRG	4 cut(s)	455, 1622, 1629, 1748
Bsp120I	GGGCCC	1 cut(s)	628
Bsp1286I	GDGCHC	7 cut(s)	54, 558, 632, 689, 1689, 1728, 1884
Bsp1407I	TGTACA	2 cut(s)	1299, 1395
Bsp1720I	GCTNAGC	1 cut(s)	1142
Bsp19I	CCATGG	2 cut(s)	1312, 1346
BspANI	GGCC	4 cut(s)	314, 630, 1130, 1628
BspCNI	CTCAG	9 cut(s)	616, 1017, 1134, 1177, 1224, 1384, 1666, 1786, 1870
BspFNI	CGCG	3 cut(s)	638, 879, 1160
BspPI	GGATC	9 cut(s)	115, 128, 140, 619, 749, 762, 1111, 1447, 1880
BspQI	GCTCTTC	1 cut(s)	1046
BspT107I	GGYRCC	3 cut(s)	1114, 1517, 1618
BsrBI	CCGCTC	1 cut(s)	798
BsrDI	GCAATG	4 cut(s)	1294, 1340, 1860, 1952
BsrFI	RCCGGY	2 cut(s)	182, 310
BsrGI	TGTACA	2 cut(s)	1299, 1395
BsrI	ACTGG	3 cut(s)	167, 232, 1708
BssAI	RCCGGY	2 cut(s)	182, 310
BssECI	CCNNGG	6 cut(s)	625, 1312, 1346, 1488, 1623, 1901
BssNI	GRCGYC	5 cut(s)	171, 333, 1115, 1155, 1619
BssSI	CACGAG	2 cut(s)	368, 557
BssT1I	CCWWGG	3 cut(s)	1312, 1346, 1901
Bst2BI	CACGAG	2 cut(s)	368, 557
Bst2UI	CCWGG	3 cut(s)	133, 566, 626
Bst4CI	ACNGT	3 cut(s)	56, 814, 1915
Bst6I	CTCTTC	2 cut(s)	326, 1046
BstACI	GRCGYC	5 cut(s)	171, 333, 1115, 1155, 1619
BstAUI	TGTACA	2 cut(s)	1299, 1395
BstBAI	YACGTR	2 cut(s)	1042, 1071
BstC8I	GCNNGC	3 cut(s)	178, 312, 618
BstDEI	CTNAG	9 cut(s)	603, 1025, 1142, 1185, 1232, 1371, 1653, 1794, 1878
BstDSI	CCRYGG	2 cut(s)	1312, 1346
BstEII	GGTNACC	1 cut(s)	251
BstF5I	GGATG	3 cut(s)	736, 958, 1911
BstFNI	CGCG	3 cut(s)	638, 879, 1160
BstH2I	RGCGCY	3 cut(s)	1118, 1622, 1786
BstHHI	GCGC	3 cut(s)	1117, 1621, 1785
BstMAI	GTCTC	3 cut(s)	62, 465, 1921
BstMWI	GCNNNNNNNGC	7 cut(s)	13, 177, 228, 885, 1293, 1342, 1625
BstNI	CCWGG	3 cut(s)	133, 566, 626
BstPI	GGTNACC	1 cut(s)	251
BstSCI	CCNGG	8 cut(s)	131, 381, 564, 624, 687, 750, 1085, 1487
BstSLI	GKGCMC	3 cut(s)	54, 632, 689
BstSNI	TACGTA	1 cut(s)	1071
BstUI	CGCG	3 cut(s)	638, 879, 1160
BstV1I	GCAGC	5 cut(s)	3, 793, 858, 1305, 1653
BstV2I	GAAGAC	2 cut(s)	420, 1772
BstX2I	RGATCY	5 cut(s)	120, 754, 1103, 1452, 1695
BstXI	CCANNNNNNTGG	2 cut(s)	1347, 1615
BstYI	RGATCY	5 cut(s)	120, 754, 1103, 1452, 1695
Bsu36I	CCTNAGG	1 cut(s)	603
BsuRI	GGCC	4 cut(s)	314, 630, 1130, 1628
BtgI	CCRYGG	2 cut(s)	1312, 1346
BtgZI	GCGATG	1 cut(s)	1211
BtrI	CACGTC	1 cut(s)	1505
BtsCI	GGATG	3 cut(s)	736, 958, 1911
BtsI	GCAGTG	1 cut(s)	858
BtsIMutI	CAGTG	7 cut(s)	52, 160, 225, 407, 813, 858, 1875
Cac8I	GCNNGC	3 cut(s)	178, 312, 618
CfoI	GCGC	3 cut(s)	1117, 1621, 1785
Cfr10I	RCCGGY	2 cut(s)	182, 310
CseI	GACGC	6 cut(s)	160, 341, 863, 1163, 1530, 1886
CviAII	CATG	6 cut(s)	301, 307, 1313, 1347, 1403, 1430
DdeI	CTNAG	9 cut(s)	603, 1025, 1142, 1185, 1232, 1371, 1653, 1794, 1878
DinI	GGCGCC	2 cut(s)	1116, 1620
DrdI	GACNNNNNNGTC	1 cut(s)	1752
DriI	GACNNNNNGTC	1 cut(s)	133
DseDI	GACNNNNNNGTC	1 cut(s)	1752
EaeI	YGGCCR	1 cut(s)	1128
Eam1104I	CTCTTC	2 cut(s)	326, 1046
Eam1105I	GACNNNNNGTC	1 cut(s)	133
EarI	CTCTTC	2 cut(s)	326, 1046
EciI	GGCGGA	4 cut(s)	537, 559, 1205, 1217
Ecl136II	GAGCTC	1 cut(s)	1882
Eco105I	TACGTA	1 cut(s)	1071
Eco130I	CCWWGG	3 cut(s)	1312, 1346, 1901
Eco24I	GRGCYC	3 cut(s)	632, 1728, 1884
Eco47I	GGWCC	6 cut(s)	374, 385, 568, 1210, 1377, 1592
Eco53kI	GAGCTC	1 cut(s)	1882
Eco57I	CTGAAG	2 cut(s)	63, 1521
Eco81I	CCTNAGG	1 cut(s)	603
Eco88I	CYCGRG	4 cut(s)	455, 1622, 1629, 1748
Eco91I	GGTNACC	1 cut(s)	251
EcoICRI	GAGCTC	1 cut(s)	1882
EcoO109I	RGGNCCY	2 cut(s)	628, 629
EcoO65I	GGTNACC	1 cut(s)	251
EcoRII	CCWGG	3 cut(s)	131, 564, 624
EcoT14I	CCWWGG	3 cut(s)	1312, 1346, 1901
EcoT38I	GRGCYC	3 cut(s)	632, 1728, 1884
EgeI	GGCGCC	2 cut(s)	1116, 1620
EheI	GGCGCC	2 cut(s)	1116, 1620
ErhI	CCWWGG	3 cut(s)	1312, 1346, 1901
FaeI	CATG	6 cut(s)	304, 310, 1316, 1350, 1406, 1433
FaqI	GGGAC	2 cut(s)	1390, 1758
FatI	CATG	6 cut(s)	300, 306, 1312, 1346, 1402, 1429
FauI	CCCGC	2 cut(s)	656, 1088
FblI	GTMKAC	1 cut(s)	1445
Fnu4HI	GCNGC	8 cut(s)	17, 782, 799, 872, 1113, 1158, 1294, 1642
FokI	GGATG	3 cut(s)	743, 965, 1918
FriOI	GRGCYC	3 cut(s)	632, 1728, 1884
Fsp4HI	GCNGC	8 cut(s)	17, 782, 799, 872, 1113, 1158, 1294, 1642
FspBI	CTAG	1 cut(s)	654
GlaI	GCGC	3 cut(s)	1116, 1620, 1784
GluI	GCNGC	8 cut(s)	17, 782, 799, 872, 1113, 1158, 1294, 1642
HaeII	RGCGCY	3 cut(s)	1118, 1622, 1786
HaeIII	GGCC	4 cut(s)	314, 630, 1130, 1628
HgaI	GACGC	6 cut(s)	160, 341, 863, 1163, 1530, 1886
HhaI	GCGC	3 cut(s)	1117, 1621, 1785
Hin1I	GRCGYC	5 cut(s)	171, 333, 1115, 1155, 1619
Hin1II	CATG	6 cut(s)	304, 310, 1316, 1350, 1406, 1433
Hin6I	GCGC	3 cut(s)	1115, 1619, 1783
HinP1I	GCGC	3 cut(s)	1115, 1619, 1783
HincII	GTYRAC	1 cut(s)	1446
HindII	GTYRAC	1 cut(s)	1446
HphI	GGTGA	6 cut(s)	187, 202, 245, 580, 1163, 1522
Hpy166II	GTNNAC	8 cut(s)	52, 991, 1213, 1396, 1446, 1530, 1650, 1669
Hpy188III	TCNNGA	9 cut(s)	41, 347, 559, 1545, 1699, 1750, 1762, 1889, 1928
Hpy8I	GTNNAC	8 cut(s)	52, 991, 1213, 1396, 1446, 1530, 1650, 1669
Hpy99I	CGWCG	5 cut(s)	176, 335, 879, 1123, 1546
HpyAV	CCTTC	7 cut(s)	889, 956, 1093, 1131, 1198, 1466, 1701
HpyCH4III	ACNGT	3 cut(s)	56, 814, 1915
HpyCH4IV	ACGT	3 cut(s)	1041, 1070, 1504
HpyCH4V	TGCA	8 cut(s)	19, 52, 180, 1307, 1819, 1832, 1875, 1945
HpyF10VI	GCNNNNNNNGC	7 cut(s)	13, 177, 228, 885, 1293, 1342, 1625
HpyF3I	CTNAG	9 cut(s)	603, 1025, 1142, 1185, 1232, 1371, 1653, 1794, 1878
HpySE526I	ACGT	3 cut(s)	1041, 1070, 1504
Hsp92I	GRCGYC	5 cut(s)	171, 333, 1115, 1155, 1619
Hsp92II	CATG	6 cut(s)	304, 310, 1316, 1350, 1406, 1433
HspAI	GCGC	3 cut(s)	1115, 1619, 1783
KasI	GGCGCC	2 cut(s)	1114, 1618
KpnI	GGTACC	1 cut(s)	1521
KroI	GCCGGC	1 cut(s)	310
KroNI	GCCGGC	1 cut(s)	312
LguI	GCTCTTC	1 cut(s)	1046
LmnI	GCTCC	2 cut(s)	452, 795
Lsp1109I	GCAGC	5 cut(s)	3, 793, 858, 1305, 1653
LweI	GCATC	3 cut(s)	1040, 1840, 1944
MaeI	CTAG	1 cut(s)	654
MaeII	ACGT	3 cut(s)	1041, 1070, 1504
MaeIII	GTNAC	5 cut(s)	193, 251, 808, 1151, 1270
MbiI	CCGCTC	1 cut(s)	798
MboII	GAAGA	5 cut(s)	313, 420, 1063, 1593, 1777
MflI	RGATCY	5 cut(s)	120, 754, 1103, 1452, 1695
MhlI	GDGCHC	7 cut(s)	54, 558, 632, 689, 1689, 1728, 1884
MluCI	AATT	9 cut(s)	21, 512, 678, 822, 1010, 1319, 1365, 1680, 1870
Mly113I	GGCGCC	2 cut(s)	1115, 1619
MlyI	GAGTC	9 cut(s)	66, 122, 201, 254, 267, 467, 1740, 1762, 1933
MmeI	TCCRAC	6 cut(s)	117, 381, 654, 987, 1474, 1498
MroNI	GCCGGC	1 cut(s)	310
MroXI	GAANNNNTTC	1 cut(s)	1769
MseI	TTAA	5 cut(s)	1322, 1361, 1440, 1869, 1951
MslI	CAYNNNNRTG	2 cut(s)	305, 1345
MspR9I	CCNGG	8 cut(s)	133, 383, 566, 626, 689, 752, 1087, 1489
MvaI	CCWGG	3 cut(s)	133, 566, 626
MvnI	CGCG	3 cut(s)	638, 879, 1160
MwoI	GCNNNNNNNGC	7 cut(s)	13, 177, 228, 885, 1293, 1342, 1625
NaeI	GCCGGC	1 cut(s)	312
NarI	GGCGCC	2 cut(s)	1115, 1619
NciI	CCSGG	5 cut(s)	383, 689, 752, 1087, 1489
NcoI	CCATGG	2 cut(s)	1312, 1346
NgoMIV	GCCGGC	1 cut(s)	310
NlaIII	CATG	6 cut(s)	304, 310, 1316, 1350, 1406, 1433
NmeAIII	GCCGAG	1 cut(s)	493
NmuCI	GTSAC	4 cut(s)	193, 251, 808, 1151
PaeR7I	CTCGAG	1 cut(s)	1748
PciSI	GCTCTTC	1 cut(s)	1046
PcsI	WCGNNNNNNNCGW	1 cut(s)	999
PdiI	GCCGGC	1 cut(s)	312
PdmI	GAANNNNTTC	1 cut(s)	1769
PfeI	GAWTC	5 cut(s)	140, 547, 667, 707, 1561
Pfl23II	CGTACG	2 cut(s)	537, 1038
PfoI	TCCNGGA	1 cut(s)	381
PkrI	GCNGC	8 cut(s)	18, 783, 800, 873, 1114, 1159, 1295, 1643
PleI	GAGTC	9 cut(s)	66, 122, 200, 254, 267, 466, 1740, 1761, 1932
PluTI	GGCGCC	2 cut(s)	1118, 1622
PpsI	GAGTC	9 cut(s)	66, 122, 200, 254, 267, 466, 1740, 1761, 1932
Ppu21I	YACGTR	2 cut(s)	1042, 1071
Psp124BI	GAGCTC	1 cut(s)	1884
Psp6I	CCWGG	3 cut(s)	131, 564, 624
PspEI	GGTNACC	1 cut(s)	251
PspGI	CCWGG	3 cut(s)	131, 564, 624
PspLI	CGTACG	2 cut(s)	537, 1038
PspOMI	GGGCCC	1 cut(s)	628
PsrI	GAACNNNNNNTAC	2 cut(s)	488, 520
PsuI	RGATCY	5 cut(s)	120, 754, 1103, 1452, 1695
RseI	CAYNNNNRTG	2 cut(s)	305, 1345
SacI	GAGCTC	1 cut(s)	1884
SalI	GTCGAC	1 cut(s)	1444
SapI	GCTCTTC	1 cut(s)	1046
SaqAI	TTAA	5 cut(s)	1322, 1361, 1440, 1869, 1951
SatI	GCNGC	8 cut(s)	17, 782, 799, 872, 1113, 1158, 1294, 1642
SchI	GAGTC	9 cut(s)	66, 122, 201, 254, 267, 467, 1740, 1762, 1933
ScrFI	CCNGG	8 cut(s)	133, 383, 566, 626, 689, 752, 1087, 1489
SduI	GDGCHC	7 cut(s)	54, 558, 632, 689, 1689, 1728, 1884
SfaNI	GCATC	3 cut(s)	1040, 1840, 1944
SfoI	GGCGCC	2 cut(s)	1116, 1620
Sfr274I	CTCGAG	1 cut(s)	1748
SinI	GGWCC	6 cut(s)	374, 385, 568, 1210, 1377, 1592
SlaI	CTCGAG	1 cut(s)	1748
SmiMI	CAYNNNNRTG	2 cut(s)	305, 1345
SmlI	CTYRAG	3 cut(s)	266, 401, 1748
SmoI	CTYRAG	3 cut(s)	266, 401, 1748
SnaBI	TACGTA	1 cut(s)	1071
Sse9I	AATT	9 cut(s)	21, 512, 678, 822, 1010, 1319, 1365, 1680, 1870
SspDI	GGCGCC	2 cut(s)	1114, 1618
SspMI	CTAG	1 cut(s)	654
SstI	GAGCTC	1 cut(s)	1884
StyD4I	CCNGG	8 cut(s)	131, 381, 564, 624, 687, 750, 1085, 1487
StyI	CCWWGG	3 cut(s)	1312, 1346, 1901
TaaI	ACNGT	3 cut(s)	56, 814, 1915
TaiI	ACGT	3 cut(s)	1044, 1073, 1507
TasI	AATT	9 cut(s)	21, 512, 678, 822, 1010, 1319, 1365, 1680, 1870
TatI	WGTACW	4 cut(s)	1299, 1395, 1668, 1810
TauI	GCSGC	3 cut(s)	801, 1115, 1160
TfiI	GAWTC	5 cut(s)	140, 547, 667, 707, 1561
Tru1I	TTAA	5 cut(s)	1322, 1361, 1440, 1869, 1951
Tru9I	TTAA	5 cut(s)	1322, 1361, 1440, 1869, 1951
TscAI	CASTG	7 cut(s)	59, 167, 232, 414, 813, 858, 1882
TseFI	GTSAC	4 cut(s)	193, 251, 808, 1151
TseI	GCWGC	5 cut(s)	16, 781, 871, 1293, 1641
Tsp45I	GTSAC	4 cut(s)	193, 251, 808, 1151
TspDTI	ATGAA	4 cut(s)	1270, 1418, 1419, 1922
TspGWI	ACGGA	4 cut(s)	133, 660, 672, 784
TspRI	CASTG	7 cut(s)	59, 167, 232, 414, 813, 858, 1882
VneI	GTGCAC	1 cut(s)	50
VpaK11BI	GGWCC	6 cut(s)	374, 385, 568, 1210, 1377, 1592
XapI	RAATTY	2 cut(s)	512, 678
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1344
XhoI	CTCGAG	1 cut(s)	1748
XmiI	GTMKAC	1 cut(s)	1445
XmnI	GAANNNNTTC	1 cut(s)	1769
XspI	CTAG	1 cut(s)	654

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.