Rroxscaffold_6G00401170
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0008482
Physical Location & Seq
Forward (+)
23487056 .. 23491814
4759 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00401170.1

Sequence Viewer

Length: 1959 bp
ATGCTCAACTTTCTGCAGCTCGCTCTCCTTGTTTTTCCTGCCGTCGTTTTGGGTCTCAACTCAGACGGGCTCTCCCTCCTGGCGCTCAAAGCCGCAATCGAGACAGACCCGAGTCGAGTCCTGGACTCCTGGTCCGACTCCGACTCCACCCCGTGTCACTGGCACGGCGTCGTTTGCACCCGCGACCGAGTCACCGACCTTTTCCTCACCCAAAAGGGCTTCAACGGCTACATTCCCTCAGAACTTGGCCGCCTCGACTCACTCAAGCGACTCAACCTCGCCCGCAACAACTTCTCCAAGCCCATTCCGGCCCACCTCTTCAACGCCACCTCCCTCATTGCCCTCGACCTCTCCAGCAACTCCCTCTCCGGCCCAGTTCCGGCCCAAATCGGCTTCCTCAAAGACCTCCGCCACCTGGACTTGTCCTCCAATCTCCTCAACGGGTCTCTCCCGGAATCTCTCACCGAGCTCGAGAGCCTCGCCGGGACTTTGAACCTGTCGTACAACCAATTCTCCGGCGGTGTTCCGGCGTCGTACGGACGGCTTCCGGTACTGGTGAGCTTGGACCTCCGGCATAATAACCTCACCGGAAAGTTGCCTCAGGTGGGCTCGCTGGTGAACCAAGGCCCGACGGCGTTCTCCGGGAACCCGAGCTTGTGTGGGTTTCCGTTGGAGGTTCCGTGCCCGGAAGCCGAAAACCCGTCGGGAACCGGAGCCCAGGAGGCCCAAAAGCCGCTCGACCCGGACCCGAACCCGAATCTGCTCAATGGGGTTGAAGGGAGGGCCAAACAGAGAGGCGGGTCGGTGACAGTGCCGATAATCTCCGGCATTTCGCTGGTGATCGGGGCCGTCTCGGTCTCGGTGTGGCTGCTCCGGAAAAAGAGAACGGCCAGGGAGGGCAAAATGGGGAAAGAGAGAGTCCAAAATGAGGCGGTTGCGGAAGAGGAGGAAGGGCAAAAGGGTAAGTTCGTGGTGATGGACGAGGGGTTCGGGCTGGAGTTGGAGGACTTGCTGAGGGCGTCGGCTTACGTGGTGGGAAAAAGTAGGAGTGGGATCGTCTACAGGGTGGTTGCCGGGAATGTGGGGAAAGTAACGGGTGCGGCGGCGGCGCCGGCGCCGGCGCCGTCGGTTGTGGCCGTGAGGCGGCTGAGCGAGGGTGATGCCACGTGGCGGTTGAAGGAGTTCGAGTCTGAGGTGGAGGCTATTGGGAAGGTGGTCCACCCGAATATCGTGCGGTTGAGAGCTTATTACTATGCAGATGACGAGAAGCTCTTGATTTCTGATTTCATTCGCAATGGAAGCCTGTACACTGCGCTACACGGGGGACCAACCAATTCTTCTACACCGTTACCATGGGCAGCTAGGTTAAAAATTGCTCAGGGGAGCGCAAGAGGTTTGACATACATACACGAACACAGCCCTAGAAAGTATGTTCACGGCAACATCAAATCAACAAAAATCCTTCTCAATGAGGACCTCCAGCCTTACGTATCTGGTTTCGGATTGGCACGTCTTATCTTGGGGTCCTCAAAGTTTACTTCTTCAGCTTCCAGGAAACAGAACTCGAGCCAATCCAATGCAACCTCTTGTTTTACTGTTTCAACTCACTCAACCATTTACTTGGCACCTGAGGCTCGAATTTCTGGCAGCAAGTTTACTCAGAAATGTGATGTGTACTCTTTTGGGATAGTGCTCATGGAGATTTTAACCGGTAAGCTACCAGATGAGGGGCCCGAAAATGGTGGGAAGGGACTAGAGAGTCTGGTGAGGAAGACATTTCGAGAAGAACGCCCCTTATCCGAGATTATAGACCCTGCACTTCTGCAAGAGGTTTATGCAAAGAAGCAAGTTGTTGAAGCCTTTCACATTGCTCTTAATTGCACTGAACTCGACCCAGATCTGCGTCCTAGGATGAAAACAGTTTCGGACAGTCTTGATTGCATCAAATTGCAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

652

Amino Acids

70.41

Weight (kDa)

8.0

Isoelectric Point (pI)

37.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 19 - 60 8e-12 Leucine rich repeat N-terminal domain
LRR_8 PF13855 87 - 146 3.8e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 358 - 644 1.8e-27 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 359 - 639 3e-23 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1758
AccB1I GGYRCC 4 cut(s) 1108, 1114, 1120, 1624
AccBSI CCGCTC 1 cut(s) 736
AccI GTMKAC 1 cut(s) 1059
AccII CGCG 1 cut(s) 183
AccIII TCCGGA 1 cut(s) 873
AclWI GGATC 1 cut(s) 1061
AcoI YGGCCR 3 cut(s) 247, 888, 1134
AcsI RAATTY 1 cut(s) 1638
AcuI CTGAAG 1 cut(s) 1527
AcvI CACGTG 1 cut(s) 1167
AcyI GRCGYC 6 cut(s) 168, 530, 1019, 1109, 1115, 1121
AdeI CACNNNGTG 1 cut(s) 153
AfaI GTAC 5 cut(s) 503, 536, 552, 1307, 1676
AfiI CCNNNNNNNGG 8 cut(s) 379, 415, 605, 928, 1143, 1170, 1727, 1739
AgeI ACCGGT 1 cut(s) 1709
AgsI TTSAA 7 cut(s) 223, 322, 493, 776, 1177, 1602, 1856
AhdI GACNNNNNGTC 1 cut(s) 130
AjiI CACGTC 1 cut(s) 1511
AjnI CCWGG 7 cut(s) 78, 120, 128, 414, 717, 890, 1550
AjuI GAANNNNNNNTTGG 2 cut(s) 1321, 1353
AloI GAACNNNNNNTCC 2 cut(s) 971, 1003
Alw21I GWGCWC 2 cut(s) 471, 1695
Alw26I GTCTC 5 cut(s) 59, 95, 450, 856, 862
AlwI GGATC 1 cut(s) 1061
Ama87I CYCGRG 4 cut(s) 109, 470, 649, 1564
Aor13HI TCCGGA 1 cut(s) 873
ApaI GGGCCC 1 cut(s) 1734
ApeKI GCWGC 5 cut(s) 16, 868, 1358, 1647, 1951
ApoI RAATTY 1 cut(s) 1638
ArsI GACNNNNNNTTYG 2 cut(s) 971, 1003
AsiGI ACCGGT 1 cut(s) 1709
Asp700I GAANNNNTTC 2 cut(s) 1181, 1860
AspA2I CCTAGG 1 cut(s) 1907
AspLEI GCGC 6 cut(s) 85, 1111, 1117, 1123, 1315, 1388
AsuC2I CCSGG 6 cut(s) 452, 484, 643, 686, 743, 1075
AvaI CYCGRG 4 cut(s) 109, 470, 649, 1564
AvaII GGWCC 7 cut(s) 132, 565, 745, 1216, 1325, 1474, 1524
AvrII CCTAGG 1 cut(s) 1907
AxyI CCTNAGG 2 cut(s) 600, 1629
BaeGI GKGCMC 2 cut(s) 686, 1734
BanI GGYRCC 4 cut(s) 1108, 1114, 1120, 1624
BanII GRGCYC 5 cut(s) 72, 471, 611, 718, 1734
BbrPI CACGTG 1 cut(s) 1167
BbsI GAAGAC 1 cut(s) 1778
Bbv12I GWGCWC 2 cut(s) 471, 1695
BbvCI CCTCAGC 1 cut(s) 1013
BbvI GCAGC 4 cut(s) 28, 855, 1370, 1659
BccI CCATC 1 cut(s) 970
BcgI CGANNNNNNTGC 6 cut(s) 1142, 1176, 1213, 1247, 1870, 1904
BciT130I CCWGG 7 cut(s) 80, 122, 130, 416, 719, 892, 1552
BcnI CCSGG 6 cut(s) 452, 484, 643, 686, 743, 1075
BcoDI GTCTC 5 cut(s) 59, 95, 450, 856, 862
BfaI CTAG 4 cut(s) 1362, 1422, 1754, 1908
BfmI CTRYAG 2 cut(s) 14, 1060
BfoI RGCGCY 4 cut(s) 86, 1112, 1118, 1124
BglI GCCNNNNNGGC 1 cut(s) 722
BglII AGATCT 1 cut(s) 1897
BlnI CCTAGG 1 cut(s) 1907
BlpI GCTNAGC 1 cut(s) 1148
Bme18I GGWCC 7 cut(s) 132, 565, 745, 1216, 1325, 1474, 1524
BmeRI GACNNNNNGTC 1 cut(s) 130
BmeT110I CYCGRG 4 cut(s) 109, 470, 649, 1564
BmgBI CACGTC 1 cut(s) 1511
BmrI ACTGGG 1 cut(s) 368
BmsI GCATC 2 cut(s) 1150, 1950
BmuI ACTGGG 1 cut(s) 368
BoxI GACNNNNGTC 1 cut(s) 111
BpiI GAAGAC 1 cut(s) 1778
BpmI CTGGAG 3 cut(s) 337, 1016, 1463
Bpu10I CCTNAGC 2 cut(s) 1013, 1377
Bpu1102I GCTNAGC 1 cut(s) 1148
BpuEI CTTGAG 1 cut(s) 248
BpuMI CCSGG 6 cut(s) 452, 484, 643, 686, 743, 1075
BsaAI YACGTR 3 cut(s) 1030, 1167, 1489
BsaHI GRCGYC 6 cut(s) 168, 530, 1019, 1109, 1115, 1121
BsaI GGTCTC 3 cut(s) 59, 450, 862
BsaJI CCNNGG 5 cut(s) 622, 717, 891, 1352, 1907
BsaWI WCCGGW 5 cut(s) 547, 587, 710, 873, 1709
Bsc4I CCNNNNNNNGG 8 cut(s) 379, 415, 605, 928, 1143, 1170, 1727, 1739
Bse118I RCCGGY 3 cut(s) 1111, 1117, 1709
Bse1I ACTGG 3 cut(s) 164, 374, 558
Bse21I CCTNAGG 2 cut(s) 600, 1629
Bse3DI GCAATG 3 cut(s) 336, 1300, 1866
BseAI TCCGGA 1 cut(s) 873
BseBI CCWGG 7 cut(s) 80, 122, 130, 416, 719, 892, 1552
BseDI CCNNGG 5 cut(s) 622, 717, 891, 1352, 1907
BseGI GGATG 1 cut(s) 1917
BseLI CCNNNNNNNGG 8 cut(s) 379, 415, 605, 928, 1143, 1170, 1727, 1739
BseMI GCAATG 3 cut(s) 336, 1300, 1866
BseMII CTCAG 9 cut(s) 75, 252, 614, 1004, 1139, 1182, 1391, 1620, 1673
BseNI ACTGG 3 cut(s) 164, 374, 558
BseRI GAGGAG 2 cut(s) 425, 959
BseSI GKGCMC 2 cut(s) 686, 1734
BseXI GCAGC 4 cut(s) 28, 855, 1370, 1659
BsgI GTGCAG 1 cut(s) 1800
Bsh1236I CGCG 1 cut(s) 183
Bsh1285I CGRYCG 1 cut(s) 187
BshNI GGYRCC 4 cut(s) 1108, 1114, 1120, 1624
BshTI ACCGGT 1 cut(s) 1709
BsiEI CGRYCG 1 cut(s) 187
BsiHKAI GWGCWC 2 cut(s) 471, 1695
BsiHKCI CYCGRG 4 cut(s) 109, 470, 649, 1564
BsiWI CGTACG 1 cut(s) 534
BslFI GGGAC 3 cut(s) 499, 1338, 1764
BslI CCNNNNNNNGG 8 cut(s) 379, 415, 605, 928, 1143, 1170, 1727, 1739
BsmAI GTCTC 5 cut(s) 59, 95, 450, 856, 862
BsmBI CGTCTC 1 cut(s) 856
BsmFI GGGAC 3 cut(s) 499, 1338, 1764
Bso31I GGTCTC 3 cut(s) 59, 450, 862
BsoBI CYCGRG 4 cut(s) 109, 470, 649, 1564
Bsp120I GGGCCC 1 cut(s) 1730
Bsp1286I GDGCHC 7 cut(s) 72, 471, 611, 686, 718, 1695, 1734
Bsp13I TCCGGA 1 cut(s) 873
Bsp1407I TGTACA 1 cut(s) 1305
Bsp143I GATC 3 cut(s) 840, 1053, 1897
Bsp1720I GCTNAGC 1 cut(s) 1148
Bsp19I CCATGG 1 cut(s) 1352
BspCNI CTCAG 9 cut(s) 74, 251, 613, 1005, 1140, 1183, 1390, 1621, 1672
BspEI TCCGGA 1 cut(s) 873
BspFNI CGCG 1 cut(s) 183
BspMAI CTGCAG 1 cut(s) 18
BspPI GGATC 1 cut(s) 1061
BspT107I GGYRCC 4 cut(s) 1108, 1114, 1120, 1624
BspTNI GGTCTC 3 cut(s) 59, 450, 862
BsrBI CCGCTC 1 cut(s) 736
BsrDI GCAATG 3 cut(s) 336, 1300, 1866
BsrFI RCCGGY 3 cut(s) 1111, 1117, 1709
BsrGI TGTACA 1 cut(s) 1305
BsrI ACTGG 3 cut(s) 164, 374, 558
BssAI RCCGGY 3 cut(s) 1111, 1117, 1709
BssECI CCNNGG 5 cut(s) 622, 717, 891, 1352, 1907
BssMI GATC 3 cut(s) 840, 1053, 1897
BssNI GRCGYC 6 cut(s) 168, 530, 1019, 1109, 1115, 1121
BssT1I CCWWGG 3 cut(s) 622, 1352, 1907
Bst2UI CCWGG 7 cut(s) 80, 122, 130, 416, 719, 892, 1552
Bst4CI ACNGT 5 cut(s) 811, 1347, 1597, 1921, 1931
Bst6I CTCTTC 2 cut(s) 323, 936
BstACI GRCGYC 6 cut(s) 168, 530, 1019, 1109, 1115, 1121
BstAUI TGTACA 1 cut(s) 1305
BstBAI YACGTR 3 cut(s) 1030, 1167, 1489
BstC8I GCNNGC 5 cut(s) 21, 283, 611, 1113, 1119
BstDEI CTNAG 9 cut(s) 61, 238, 600, 1013, 1148, 1191, 1377, 1629, 1659
BstDSI CCRYGG 1 cut(s) 1352
BstF5I GGATG 1 cut(s) 1917
BstFNI CGCG 1 cut(s) 183
BstH2I RGCGCY 4 cut(s) 86, 1112, 1118, 1124
BstHHI GCGC 6 cut(s) 85, 1111, 1117, 1123, 1315, 1388
BstKTI GATC 3 cut(s) 843, 1056, 1900
BstMAI GTCTC 5 cut(s) 59, 95, 450, 856, 862
BstMBI GATC 3 cut(s) 840, 1053, 1897
BstMCI CGRYCG 1 cut(s) 187
BstMWI GCNNNNNNNGC 8 cut(s) 89, 174, 225, 722, 1106, 1112, 1299, 1631
BstNI CCWGG 7 cut(s) 80, 122, 130, 416, 719, 892, 1552
BstPAI GACNNNNGTC 1 cut(s) 111
BstSFI CTRYAG 2 cut(s) 14, 1060
BstSLI GKGCMC 2 cut(s) 686, 1734
BstSNI TACGTA 1 cut(s) 1489
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 4 cut(s) 28, 855, 1370, 1659
BstV2I GAAGAC 1 cut(s) 1778
BstX2I RGATCY 1 cut(s) 1897
BstXI CCANNNNNNTGG 1 cut(s) 1621
BstYI RGATCY 1 cut(s) 1897
Bsu36I CCTNAGG 2 cut(s) 600, 1629
BtgI CCRYGG 1 cut(s) 1352
BtrI CACGTC 1 cut(s) 1511
BtsCI GGATG 1 cut(s) 1917
BtsI GCAGTG 1 cut(s) 1308
BtsIMutI CAGTG 4 cut(s) 157, 816, 1308, 1881
Cac8I GCNNGC 5 cut(s) 21, 283, 611, 1113, 1119
CfoI GCGC 6 cut(s) 85, 1111, 1117, 1123, 1315, 1388
Cfr10I RCCGGY 3 cut(s) 1111, 1117, 1709
CseI GACGC 4 cut(s) 157, 519, 1008, 1892
Csp6I GTAC 5 cut(s) 502, 535, 551, 1306, 1675
CspAI ACCGGT 1 cut(s) 1709
CviAII CATG 2 cut(s) 1353, 1696
CviQI GTAC 5 cut(s) 502, 535, 551, 1306, 1675
DdeI CTNAG 9 cut(s) 61, 238, 600, 1013, 1148, 1191, 1377, 1629, 1659
DinI GGCGCC 3 cut(s) 1110, 1116, 1122
DpnI GATC 3 cut(s) 842, 1055, 1899
DpnII GATC 3 cut(s) 840, 1053, 1897
DraIII CACNNNGTG 1 cut(s) 153
DrdI GACNNNNNNGTC 1 cut(s) 1758
DriI GACNNNNNGTC 1 cut(s) 130
DseDI GACNNNNNNGTC 1 cut(s) 1758
EaeI YGGCCR 3 cut(s) 247, 888, 1134
Eam1104I CTCTTC 2 cut(s) 323, 936
Eam1105I GACNNNNNGTC 1 cut(s) 130
EarI CTCTTC 2 cut(s) 323, 936
EciI GGCGGA 1 cut(s) 398
Ecl136II GAGCTC 1 cut(s) 469
Eco105I TACGTA 1 cut(s) 1489
Eco130I CCWWGG 3 cut(s) 622, 1352, 1907
Eco24I GRGCYC 5 cut(s) 72, 471, 611, 718, 1734
Eco31I GGTCTC 3 cut(s) 59, 450, 862
Eco47I GGWCC 7 cut(s) 132, 565, 745, 1216, 1325, 1474, 1524
Eco53kI GAGCTC 1 cut(s) 469
Eco57I CTGAAG 1 cut(s) 1527
Eco72I CACGTG 1 cut(s) 1167
Eco81I CCTNAGG 2 cut(s) 600, 1629
Eco88I CYCGRG 4 cut(s) 109, 470, 649, 1564
EcoICRI GAGCTC 1 cut(s) 469
EcoO109I RGGNCCY 3 cut(s) 1474, 1524, 1730
EcoRII CCWGG 7 cut(s) 78, 120, 128, 414, 717, 890, 1550
EcoT14I CCWWGG 3 cut(s) 622, 1352, 1907
EcoT38I GRGCYC 5 cut(s) 72, 471, 611, 718, 1734
EgeI GGCGCC 3 cut(s) 1110, 1116, 1122
EheI GGCGCC 3 cut(s) 1110, 1116, 1122
ErhI CCWWGG 3 cut(s) 622, 1352, 1907
Esp3I CGTCTC 1 cut(s) 856
FaeI CATG 2 cut(s) 1356, 1699
FaiI YATR 9 cut(s) 576, 1254, 1354, 1402, 1406, 1431, 1697, 1808, 1836
FaqI GGGAC 3 cut(s) 499, 1338, 1764
FatI CATG 2 cut(s) 1352, 1695
FauI CCCGC 3 cut(s) 188, 290, 791
FblI GTMKAC 1 cut(s) 1059
FokI GGATG 1 cut(s) 1924
FriOI GRGCYC 5 cut(s) 72, 471, 611, 718, 1734
FspBI CTAG 4 cut(s) 1362, 1422, 1754, 1908
GlaI GCGC 6 cut(s) 84, 1110, 1116, 1122, 1314, 1387
GsuI CTGGAG 3 cut(s) 337, 1016, 1463
HaeII RGCGCY 4 cut(s) 86, 1112, 1118, 1124
HgaI GACGC 4 cut(s) 157, 519, 1008, 1892
HhaI GCGC 6 cut(s) 85, 1111, 1117, 1123, 1315, 1388
Hin1I GRCGYC 6 cut(s) 168, 530, 1019, 1109, 1115, 1121
Hin1II CATG 2 cut(s) 1356, 1699
Hin6I GCGC 6 cut(s) 83, 1109, 1115, 1121, 1313, 1386
HinP1I GCGC 6 cut(s) 83, 1109, 1115, 1121, 1313, 1386
Hpy166II GTNNAC 8 cut(s) 619, 1060, 1219, 1308, 1435, 1536, 1656, 1675
Hpy188III TCNNGA 7 cut(s) 100, 472, 705, 874, 1273, 1781, 1934
Hpy8I GTNNAC 8 cut(s) 619, 1060, 1219, 1308, 1435, 1536, 1656, 1675
Hpy99I CGWCG 7 cut(s) 47, 173, 535, 634, 706, 1024, 1129
HpyAV CCTTC 6 cut(s) 770, 944, 1171, 1204, 1472, 1741
HpyCH4III ACNGT 5 cut(s) 811, 1347, 1597, 1921, 1931
HpyCH4IV ACGT 4 cut(s) 1029, 1166, 1488, 1510
HpyF10VI GCNNNNNNNGC 8 cut(s) 89, 174, 225, 722, 1106, 1112, 1299, 1631
HpyF3I CTNAG 9 cut(s) 61, 238, 600, 1013, 1148, 1191, 1377, 1629, 1659
HpySE526I ACGT 4 cut(s) 1029, 1166, 1488, 1510
Hsp92I GRCGYC 6 cut(s) 168, 530, 1019, 1109, 1115, 1121
Hsp92II CATG 2 cut(s) 1356, 1699
HspAI GCGC 6 cut(s) 83, 1109, 1115, 1121, 1313, 1386
KasI GGCGCC 3 cut(s) 1108, 1114, 1120
Kpn2I TCCGGA 1 cut(s) 873
KroI GCCGGC 2 cut(s) 1111, 1117
KroNI GCCGGC 2 cut(s) 1113, 1119
Kzo9I GATC 3 cut(s) 840, 1053, 1897
LmnI GCTCC 3 cut(s) 713, 876, 1383
Lsp1109I GCAGC 4 cut(s) 28, 855, 1370, 1659
LweI GCATC 2 cut(s) 1150, 1950
MaeI CTAG 4 cut(s) 1362, 1422, 1754, 1908
MaeII ACGT 4 cut(s) 1029, 1166, 1488, 1510
MaeIII GTNAC 5 cut(s) 155, 190, 805, 1090, 1347
MalI GATC 3 cut(s) 842, 1055, 1899
MbiI CCGCTC 1 cut(s) 736
MboI GATC 3 cut(s) 840, 1053, 1897
MboII GAAGA 6 cut(s) 310, 953, 1329, 1533, 1783, 1796
MflI RGATCY 1 cut(s) 1897
MhlI GDGCHC 7 cut(s) 72, 471, 611, 686, 718, 1695, 1734
MluCI AATT 6 cut(s) 509, 1333, 1371, 1638, 1876, 1946
Mly113I GGCGCC 3 cut(s) 1109, 1115, 1121
MmeI TCCRAC 4 cut(s) 159, 165, 651, 981
MreI CGCCGGCG 2 cut(s) 1111, 1117
MroI TCCGGA 1 cut(s) 873
MroNI GCCGGC 2 cut(s) 1111, 1117
MroXI GAANNNNTTC 2 cut(s) 1181, 1860
MseI TTAA 3 cut(s) 1367, 1706, 1875
MvaI CCWGG 7 cut(s) 80, 122, 130, 416, 719, 892, 1552
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 8 cut(s) 89, 174, 225, 722, 1106, 1112, 1299, 1631
NaeI GCCGGC 2 cut(s) 1113, 1119
NarI GGCGCC 3 cut(s) 1109, 1115, 1121
NciI CCSGG 6 cut(s) 452, 484, 643, 686, 743, 1075
NcoI CCATGG 1 cut(s) 1352
NdeII GATC 3 cut(s) 840, 1053, 1897
NgoMIV GCCGGC 2 cut(s) 1111, 1117
NlaIII CATG 2 cut(s) 1356, 1699
NmuCI GTSAC 3 cut(s) 155, 190, 805
PaeR7I CTCGAG 2 cut(s) 470, 1564
PdiI GCCGGC 2 cut(s) 1113, 1119
PdmI GAANNNNTTC 2 cut(s) 1181, 1860
PfeI GAWTC 2 cut(s) 455, 757
Pfl23II CGTACG 1 cut(s) 534
PflFI GACNNNGTC 1 cut(s) 188
PfoI TCCNGGA 4 cut(s) 120, 450, 641, 1550
PinAI ACCGGT 1 cut(s) 1709
PluTI GGCGCC 3 cut(s) 1112, 1118, 1124
PmaCI CACGTG 1 cut(s) 1167
PmlI CACGTG 1 cut(s) 1167
Ppu21I YACGTR 3 cut(s) 1030, 1167, 1489
PpuMI RGGWCCY 2 cut(s) 1474, 1524
PshAI GACNNNNGTC 1 cut(s) 111
Psp124BI GAGCTC 1 cut(s) 471
Psp5II RGGWCCY 2 cut(s) 1474, 1524
Psp6I CCWGG 7 cut(s) 78, 120, 128, 414, 717, 890, 1550
PspCI CACGTG 1 cut(s) 1167
PspGI CCWGG 7 cut(s) 78, 120, 128, 414, 717, 890, 1550
PspLI CGTACG 1 cut(s) 534
PspOMI GGGCCC 1 cut(s) 1730
PspPPI RGGWCCY 2 cut(s) 1474, 1524
PspXI VCTCGAGB 1 cut(s) 1564
PsrI GAACNNNNNNTAC 2 cut(s) 485, 517
PstI CTGCAG 1 cut(s) 18
PsuI RGATCY 1 cut(s) 1897
PsyI GACNNNGTC 1 cut(s) 188
RsaI GTAC 5 cut(s) 503, 536, 552, 1307, 1676
RsaNI GTAC 5 cut(s) 502, 535, 551, 1306, 1675
SacI GAGCTC 1 cut(s) 471
SaqAI TTAA 3 cut(s) 1367, 1706, 1875
Sau3AI GATC 3 cut(s) 840, 1053, 1897
SduI GDGCHC 7 cut(s) 72, 471, 611, 686, 718, 1695, 1734
SfaNI GCATC 2 cut(s) 1150, 1950
SfcI CTRYAG 2 cut(s) 14, 1060
SfoI GGCGCC 3 cut(s) 1110, 1116, 1122
Sfr274I CTCGAG 2 cut(s) 470, 1564
SgrAI CRCCGGYG 2 cut(s) 1111, 1117
SinI GGWCC 7 cut(s) 132, 565, 745, 1216, 1325, 1474, 1524
SlaI CTCGAG 2 cut(s) 470, 1564
SmlI CTYRAG 3 cut(s) 263, 470, 1564
SmoI CTYRAG 3 cut(s) 263, 470, 1564
SnaBI TACGTA 1 cut(s) 1489
Sse9I AATT 6 cut(s) 509, 1333, 1371, 1638, 1876, 1946
SspDI GGCGCC 3 cut(s) 1108, 1114, 1120
SspMI CTAG 4 cut(s) 1362, 1422, 1754, 1908
SstI GAGCTC 1 cut(s) 471
StyI CCWWGG 3 cut(s) 622, 1352, 1907
TaaI ACNGT 5 cut(s) 811, 1347, 1597, 1921, 1931
TaiI ACGT 4 cut(s) 1032, 1169, 1491, 1513
TaqII GACCGA 2 cut(s) 201, 844
TasI AATT 6 cut(s) 509, 1333, 1371, 1638, 1876, 1946
TatI WGTACW 2 cut(s) 1305, 1674
TauI GCSGC 7 cut(s) 95, 252, 736, 1103, 1106, 1109, 1147
TfiI GAWTC 2 cut(s) 455, 757
Tru1I TTAA 3 cut(s) 1367, 1706, 1875
Tru9I TTAA 3 cut(s) 1367, 1706, 1875
TscAI CASTG 4 cut(s) 164, 816, 1315, 1888
TseFI GTSAC 3 cut(s) 155, 190, 805
TseI GCWGC 5 cut(s) 16, 868, 1358, 1647, 1951
Tsp45I GTSAC 3 cut(s) 155, 190, 805
TspDTI ATGAA 2 cut(s) 1276, 1928
TspGWI ACGGA 3 cut(s) 552, 657, 669
TspRI CASTG 4 cut(s) 164, 816, 1315, 1888
Tth111I GACNNNGTC 1 cut(s) 188
VpaK11BI GGWCC 7 cut(s) 132, 565, 745, 1216, 1325, 1474, 1524
XapI RAATTY 1 cut(s) 1638
XhoI CTCGAG 2 cut(s) 470, 1564
XmaJI CCTAGG 1 cut(s) 1907
XmiI GTMKAC 1 cut(s) 1059
XmnI GAANNNNTTC 2 cut(s) 1181, 1860
XspI CTAG 4 cut(s) 1362, 1422, 1754, 1908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.