pycom14g05130
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0008482
Physical Location & Seq
Reverse (-)
4941627 .. 4943579
1953 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g05130.1

Sequence Viewer

Length: 1953 bp
ATGATTCTTTTTTTGCTGCAAATTGCTCTACTTCTGGTTCCTGAAGTGGTGCACTGTCTCAACCAAGATGGACTCTCCCTCCTCGCACTCAAATCCGCAATCGAAACAGACCCGACCCGGATCCTCGACTCCTGGTCGGAATCCGATCCCACCCCCTGCCACTGGCACGGCGTCGCCTGCACCGGCAACCGAGTCACCGACCTCCTCCTCACCAACAAACGCCTCACCGGCTACATTCCCTCCGAACTTGGCCACCTCGACTCGCTCAAGCGACTCAATCTTTCAAACAACAACTTCTCCATGCCCATCCCGGCCCACCTCTTCAACGCCGCCGCGCTCATTTCTCTCGACCTCTCCAACAACTCTCTCGTGGGTCCAATCCCGGACCAAATCCGATCACTCAAGGCCCTGAACCACCTCGACCTGTCTTCCAATCTCCTCAACGGCTTTCTCCCCGAGTCTCTCGCCGAGCTTCCCTCCCTCGCCGGAACCCTGAACCTCTCGTACAACAAATTCTCCGGCGGAGTTCCGGCTTCGTACGGGCGGATTCCGGTGCTCGTGAGCCTGGACCTCCGGCACAACAACCTCACCGGAAAAGTGCCTCAGGTGGGATCGCTGGCGAACCAGGGCCCCACCGCGTTTTCCGAAAACCCTAGCCTCTGCGGGATTCCGTTGGATATTCCGTGCCCGGAAGCTCAAAACCCTGATTCCCCGAAAAGCGGGGGTGAGGATGTTCAAAAGCCTCTCAACCCGGATCCCAGTTTGGTACGGAGGGCAGAGCAGCGAGAAAATCGGAGCGGCGTGTCAGTGACGGTTCCGATAATTTCCGGCGTTTCGGTGGTGGTTGGGGCCGTGTCGGTATCGGTGTGGCTGCTTCGCGTAAAAAGCAGGGCGAAGGAGGGCAAAACGGGAAAAGAGAAAGTGGTAAAGGAGGTGGCTGTCGTGGTGGAGAATGAGGGGGAAGGGCAAAATGGTACATTCGTTGTGGTGGACGAGGGGTTCGAGTTGGAATTGGAGGATTTGCTGAGGGCATCGGCGTACGTGGTGGGGAAGAGCAGGAGTGGGATTACGTACAGAGTGGTCGCTGGGAATGGAGGGAAGGGATCTGGTGCAGCGCCGTCGGTTGTCGCCGTGAGAAGGCTGAGCGAGGGTGACGCCGCGTGGCGGTTCAAGGAGTTTGAGGCTGAGGCGGAGGCGATGGGGAAGGTGGTCCACCCCAATATCGTCCGCCTGAGAGCATATTACTATGCGAATGATGAGAAACTTTTGGTTACTGATTTCATTCGCAATGGCAGCTTGTACAATGCACTCCACGGTAAATTAACCGACTCTTTGCCGCCATTGCCATGGACGGCAAGGTTAAAAATTGCTCAGGGGATCGCAAGGGGTTTGGTGTACATACATGAACACAGCCCTCGAAAGTATGTTCATGGAAGCATTAAATCGACAAAGATCCTTCTCAACGATGATCTCCAACCTTACATATCCGGGTTCGGATTGGCACGTCTTATGTTGGGTACCTCAAAGTTCTCCACTTCAGCGTCGAGAAAGCAGAACTCGAATCAATCCATTGTGGCTTCTGGTGTGGCTGGTCCAAGTTCTTCAACCATTTACTTGGCGCCCGAGGCCCGAGTTTCTGGCAGCAAGTTCACTCAGAAATGTGATGTGTACTCCTTCGGAGTTGTGCTCATGGAGATCTTGACTGGTAGGTTGCCAGATGAGGGGCTCGAAAATGGTGGCGAGGGACTCGAGAGTCTGGTCAGGAAGACATTCAGAGATGAGCGCCCCCTTTCTGAGATCATAGACCCTGTACTTGTGCAAGAAGTTCATGCAAAGAAGCAAGTTGTTGAAGCCTTTCACATTGCCCTTAATTGCACTGAGCTCGACCCCGAACTGCGTCCTAGGATGAAAACTGTCTCCGAGAGCCTTGATCGCATCAGACTGCAATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

651

Amino Acids

70.38

Weight (kDa)

6.93

Isoelectric Point (pI)

36.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 20 - 61 2.5e-11 Leucine rich repeat N-terminal domain
LRR_8 PF13855 113 - 172 6.9e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 368 - 643 6.1e-29 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 375 - 639 2e-22 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1752
Acc65I GGTACC 1 cut(s) 1517
AccB1I GGYRCC 2 cut(s) 1517, 1618
AccBSI CCGCTC 1 cut(s) 798
AccII CGCG 4 cut(s) 335, 638, 879, 1160
AclWI GGATC 9 cut(s) 115, 128, 140, 619, 749, 762, 1111, 1385, 1447
AcoI YGGCCR 1 cut(s) 250
AcsI RAATTY 1 cut(s) 512
AcuI CTGAAG 2 cut(s) 63, 1521
AcyI GRCGYC 3 cut(s) 171, 1155, 1619
AfiI CCNNNNNNNGG 6 cut(s) 162, 608, 719, 1137, 1164, 1721
AgsI TTSAA 6 cut(s) 285, 325, 737, 1171, 1605, 1850
AhdI GACNNNNNGTC 1 cut(s) 133
AjiI CACGTC 1 cut(s) 1505
AjnI CCWGG 3 cut(s) 131, 564, 624
AloI GAACNNNNNNTCC 2 cut(s) 983, 1015
AluBI AGCT 4 cut(s) 472, 695, 1296, 1882
AluI AGCT 4 cut(s) 472, 695, 1296, 1882
Alw21I GWGCWC 4 cut(s) 54, 558, 1689, 1884
Alw26I GTCTC 3 cut(s) 62, 465, 1921
Alw44I GTGCAC 1 cut(s) 50
AlwI GGATC 9 cut(s) 115, 128, 140, 619, 749, 762, 1111, 1385, 1447
Ama87I CYCGRG 4 cut(s) 455, 1622, 1629, 1748
AoxI GGCC 6 cut(s) 250, 312, 405, 628, 849, 1626
ApaI GGGCCC 1 cut(s) 632
ApaLI GTGCAC 1 cut(s) 50
ApeKI GCWGC 6 cut(s) 16, 781, 871, 1112, 1293, 1641
ApoI RAATTY 1 cut(s) 512
ArsI GACNNNNNNTTYG 2 cut(s) 983, 1015
Asp700I GAANNNNTTC 2 cut(s) 1769, 1854
Asp718I GGTACC 1 cut(s) 1517
AspA2I CCTAGG 1 cut(s) 1901
AspLEI GCGC 4 cut(s) 337, 1117, 1621, 1785
AsuC2I CCSGG 6 cut(s) 118, 311, 383, 689, 752, 1489
AsuHPI GGTGA 6 cut(s) 187, 202, 217, 580, 737, 1163
AvaI CYCGRG 4 cut(s) 455, 1622, 1629, 1748
AvaII GGWCC 5 cut(s) 374, 385, 568, 1210, 1592
AvrII CCTAGG 1 cut(s) 1901
AxyI CCTNAGG 1 cut(s) 603
BaeGI GKGCMC 3 cut(s) 54, 632, 689
BaeI ACNNNNGTAYC 2 cut(s) 966, 999
BalI TGGCCA 1 cut(s) 252
BamHI GGATCC 2 cut(s) 120, 754
BanI GGYRCC 2 cut(s) 1517, 1618
BanII GRGCYC 3 cut(s) 632, 1728, 1884
BauI CACGAG 2 cut(s) 368, 557
BbsI GAAGAC 2 cut(s) 420, 1772
Bbv12I GWGCWC 4 cut(s) 54, 558, 1689, 1884
BbvCI CCTCAGC 2 cut(s) 1025, 1185
BbvI GCAGC 6 cut(s) 3, 793, 858, 1124, 1305, 1653
BccI CCATC 3 cut(s) 62, 314, 1192
BceAI ACGGC 6 cut(s) 184, 460, 836, 1102, 1115, 1368
BcgI CGANNNNNNTGC 4 cut(s) 1101, 1135, 1864, 1898
BciT130I CCWGG 3 cut(s) 133, 566, 626
BcnI CCSGG 6 cut(s) 118, 311, 383, 689, 752, 1489
BcoDI GTCTC 3 cut(s) 62, 465, 1921
BfaI CTAG 2 cut(s) 654, 1902
BfoI RGCGCY 3 cut(s) 1118, 1622, 1786
BglI GCCNNNNNGGC 1 cut(s) 228
BglII AGATCT 1 cut(s) 1695
BlnI CCTAGG 1 cut(s) 1901
BlpI GCTNAGC 1 cut(s) 1142
Bme1390I CCNGG 9 cut(s) 118, 133, 311, 383, 566, 626, 689, 752, 1489
Bme18I GGWCC 5 cut(s) 374, 385, 568, 1210, 1592
BmeRI GACNNNNNGTC 1 cut(s) 133
BmeT110I CYCGRG 4 cut(s) 455, 1622, 1629, 1748
BmgBI CACGTC 1 cut(s) 1505
BmrFI CCNGG 9 cut(s) 118, 133, 311, 383, 566, 626, 689, 752, 1489
BmrI ACTGGG 1 cut(s) 753
BmsI GCATC 2 cut(s) 1040, 1944
BmuI ACTGGG 1 cut(s) 753
BpiI GAAGAC 2 cut(s) 420, 1772
BplI GAGNNNNNCTC 4 cut(s) 461, 493, 1671, 1703
Bpu10I CCTNAGC 3 cut(s) 1025, 1185, 1371
Bpu1102I GCTNAGC 1 cut(s) 1142
BpuEI CTTGAG 2 cut(s) 251, 386
BpuMI CCSGG 6 cut(s) 118, 311, 383, 689, 752, 1489
BsaAI YACGTR 2 cut(s) 1042, 1071
BsaHI GRCGYC 3 cut(s) 171, 1155, 1619
BsaJI CCNNGG 5 cut(s) 625, 1312, 1346, 1623, 1901
BsaWI WCCGGW 2 cut(s) 550, 590
BsaXI ACNNNNNCTCC 8 cut(s) 63, 93, 224, 254, 281, 311, 500, 530
Bsc4I CCNNNNNNNGG 6 cut(s) 162, 608, 719, 1137, 1164, 1721
Bse118I RCCGGY 2 cut(s) 182, 227
Bse1I ACTGG 3 cut(s) 167, 759, 1708
Bse21I CCTNAGG 1 cut(s) 603
Bse3DI GCAATG 4 cut(s) 1294, 1340, 1860, 1952
BseBI CCWGG 3 cut(s) 133, 566, 626
BseDI CCNNGG 5 cut(s) 625, 1312, 1346, 1623, 1901
BseGI GGATG 3 cut(s) 306, 736, 1911
BseLI CCNNNNNNNGG 6 cut(s) 162, 608, 719, 1137, 1164, 1721
BseMI GCAATG 4 cut(s) 1294, 1340, 1860, 1952
BseMII CTCAG 9 cut(s) 617, 1016, 1133, 1176, 1223, 1385, 1667, 1785, 1869
BseNI ACTGG 3 cut(s) 167, 759, 1708
BseRI GAGGAG 4 cut(s) 71, 194, 197, 428
BseSI GKGCMC 3 cut(s) 54, 632, 689
BseXI GCAGC 6 cut(s) 3, 793, 858, 1124, 1305, 1653
BseYI CCCAGC 1 cut(s) 1085
BsgI GTGCAG 2 cut(s) 163, 1131
Bsh1236I CGCG 4 cut(s) 335, 638, 879, 1160
BshFI GGCC 6 cut(s) 252, 314, 407, 630, 851, 1628
BshNI GGYRCC 2 cut(s) 1517, 1618
BsiHKAI GWGCWC 4 cut(s) 54, 558, 1689, 1884
BsiHKCI CYCGRG 4 cut(s) 455, 1622, 1629, 1748
BsiWI CGTACG 2 cut(s) 537, 1038
BslFI GGGAC 1 cut(s) 1758
BslI CCNNNNNNNGG 6 cut(s) 162, 608, 719, 1137, 1164, 1721
BsmAI GTCTC 3 cut(s) 62, 465, 1921
BsmFI GGGAC 1 cut(s) 1758
BsnI GGCC 6 cut(s) 252, 314, 407, 630, 851, 1628
BsoBI CYCGRG 4 cut(s) 455, 1622, 1629, 1748
Bsp120I GGGCCC 1 cut(s) 628
Bsp1286I GDGCHC 7 cut(s) 54, 558, 632, 689, 1689, 1728, 1884
Bsp1407I TGTACA 2 cut(s) 1299, 1395
Bsp1720I GCTNAGC 1 cut(s) 1142
Bsp19I CCATGG 1 cut(s) 1346
BspANI GGCC 6 cut(s) 252, 314, 407, 630, 851, 1628
BspCNI CTCAG 9 cut(s) 616, 1017, 1134, 1177, 1224, 1384, 1666, 1786, 1870
BspFNI CGCG 4 cut(s) 335, 638, 879, 1160
BspPI GGATC 9 cut(s) 115, 128, 140, 619, 749, 762, 1111, 1385, 1447
BspQI GCTCTTC 1 cut(s) 1046
BspT107I GGYRCC 2 cut(s) 1517, 1618
BsrBI CCGCTC 1 cut(s) 798
BsrDI GCAATG 4 cut(s) 1294, 1340, 1860, 1952
BsrFI RCCGGY 2 cut(s) 182, 227
BsrGI TGTACA 2 cut(s) 1299, 1395
BsrI ACTGG 3 cut(s) 167, 759, 1708
BssAI RCCGGY 2 cut(s) 182, 227
BssECI CCNNGG 5 cut(s) 625, 1312, 1346, 1623, 1901
BssNI GRCGYC 3 cut(s) 171, 1155, 1619
BssSI CACGAG 2 cut(s) 368, 557
BssT1I CCWWGG 2 cut(s) 1346, 1901
Bst2BI CACGAG 2 cut(s) 368, 557
Bst2UI CCWGG 3 cut(s) 133, 566, 626
Bst4CI ACNGT 4 cut(s) 56, 814, 1316, 1915
Bst6I CTCTTC 2 cut(s) 326, 1046
BstACI GRCGYC 3 cut(s) 171, 1155, 1619
BstAUI TGTACA 2 cut(s) 1299, 1395
BstBAI YACGTR 2 cut(s) 1042, 1071
BstC8I GCNNGC 2 cut(s) 178, 618
BstDEI CTNAG 9 cut(s) 603, 1025, 1142, 1185, 1232, 1371, 1653, 1794, 1878
BstDSI CCRYGG 2 cut(s) 1312, 1346
BstF5I GGATG 3 cut(s) 306, 736, 1911
BstFNI CGCG 4 cut(s) 335, 638, 879, 1160
BstH2I RGCGCY 3 cut(s) 1118, 1622, 1786
BstHHI GCGC 4 cut(s) 337, 1117, 1621, 1785
BstMAI GTCTC 3 cut(s) 62, 465, 1921
BstMWI GCNNNNNNNGC 7 cut(s) 177, 228, 885, 1293, 1342, 1625, 1932
BstNI CCWGG 3 cut(s) 133, 566, 626
BstSCI CCNGG 9 cut(s) 116, 131, 309, 381, 564, 624, 687, 750, 1487
BstSLI GKGCMC 3 cut(s) 54, 632, 689
BstSNI TACGTA 1 cut(s) 1071
BstUI CGCG 4 cut(s) 335, 638, 879, 1160
BstV1I GCAGC 6 cut(s) 3, 793, 858, 1124, 1305, 1653
BstV2I GAAGAC 2 cut(s) 420, 1772
BstX2I RGATCY 5 cut(s) 120, 754, 1103, 1452, 1695
BstXI CCANNNNNNTGG 2 cut(s) 1347, 1615
BstYI RGATCY 5 cut(s) 120, 754, 1103, 1452, 1695
Bsu36I CCTNAGG 1 cut(s) 603
BsuRI GGCC 6 cut(s) 252, 314, 407, 630, 851, 1628
BtgI CCRYGG 2 cut(s) 1312, 1346
BtgZI GCGATG 1 cut(s) 1211
BtrI CACGTC 1 cut(s) 1505
BtsCI GGATG 3 cut(s) 306, 736, 1911
BtsIMutI CAGTG 4 cut(s) 52, 160, 813, 1875
Cac8I GCNNGC 2 cut(s) 178, 618
CfoI GCGC 4 cut(s) 337, 1117, 1621, 1785
Cfr10I RCCGGY 2 cut(s) 182, 227
CseI GACGC 4 cut(s) 160, 1163, 1530, 1886
CviAII CATG 6 cut(s) 301, 1347, 1403, 1430, 1690, 1829
DdeI CTNAG 9 cut(s) 603, 1025, 1142, 1185, 1232, 1371, 1653, 1794, 1878
DinI GGCGCC 1 cut(s) 1620
DrdI GACNNNNNNGTC 1 cut(s) 1752
DriI GACNNNNNGTC 1 cut(s) 133
DseDI GACNNNNNNGTC 1 cut(s) 1752
EaeI YGGCCR 1 cut(s) 250
Eam1104I CTCTTC 2 cut(s) 326, 1046
Eam1105I GACNNNNNGTC 1 cut(s) 133
EarI CTCTTC 2 cut(s) 326, 1046
EciI GGCGGA 4 cut(s) 537, 559, 1205, 1217
Ecl136II GAGCTC 1 cut(s) 1882
Eco105I TACGTA 1 cut(s) 1071
Eco130I CCWWGG 2 cut(s) 1346, 1901
Eco24I GRGCYC 3 cut(s) 632, 1728, 1884
Eco47I GGWCC 5 cut(s) 374, 385, 568, 1210, 1592
Eco53kI GAGCTC 1 cut(s) 1882
Eco57I CTGAAG 2 cut(s) 63, 1521
Eco81I CCTNAGG 1 cut(s) 603
Eco88I CYCGRG 4 cut(s) 455, 1622, 1629, 1748
EcoICRI GAGCTC 1 cut(s) 1882
EcoO109I RGGNCCY 3 cut(s) 406, 628, 629
EcoRII CCWGG 3 cut(s) 131, 564, 624
EcoT14I CCWWGG 2 cut(s) 1346, 1901
EcoT38I GRGCYC 3 cut(s) 632, 1728, 1884
EgeI GGCGCC 1 cut(s) 1620
EheI GGCGCC 1 cut(s) 1620
ErhI CCWWGG 2 cut(s) 1346, 1901
FaeI CATG 6 cut(s) 304, 1350, 1406, 1433, 1693, 1832
FaqI GGGAC 1 cut(s) 1758
FatI CATG 6 cut(s) 300, 1346, 1402, 1429, 1689, 1828
FauI CCCGC 2 cut(s) 656, 713
FokI GGATG 3 cut(s) 293, 743, 1918
FriOI GRGCYC 3 cut(s) 632, 1728, 1884
FspBI CTAG 2 cut(s) 654, 1902
GlaI GCGC 4 cut(s) 336, 1116, 1620, 1784
GsaI CCCAGC 1 cut(s) 1089
HaeII RGCGCY 3 cut(s) 1118, 1622, 1786
HaeIII GGCC 6 cut(s) 252, 314, 407, 630, 851, 1628
HgaI GACGC 4 cut(s) 160, 1163, 1530, 1886
HhaI GCGC 4 cut(s) 337, 1117, 1621, 1785
Hin1I GRCGYC 3 cut(s) 171, 1155, 1619
Hin1II CATG 6 cut(s) 304, 1350, 1406, 1433, 1693, 1832
Hin6I GCGC 4 cut(s) 335, 1115, 1619, 1783
HinP1I GCGC 4 cut(s) 335, 1115, 1619, 1783
HphI GGTGA 6 cut(s) 187, 202, 217, 580, 737, 1163
Hpy166II GTNNAC 6 cut(s) 52, 991, 1213, 1396, 1650, 1669
Hpy188III TCNNGA 7 cut(s) 41, 347, 559, 1545, 1699, 1750, 1762
Hpy8I GTNNAC 6 cut(s) 52, 991, 1213, 1396, 1650, 1669
Hpy99I CGWCG 3 cut(s) 176, 1123, 1546
HpyAV CCTTC 7 cut(s) 889, 956, 1093, 1131, 1198, 1466, 1684
HpyCH4III ACNGT 4 cut(s) 56, 814, 1316, 1915
HpyCH4IV ACGT 3 cut(s) 1041, 1070, 1504
HpyCH4V TGCA 9 cut(s) 19, 52, 180, 1112, 1307, 1819, 1832, 1875, 1945
HpyF10VI GCNNNNNNNGC 7 cut(s) 177, 228, 885, 1293, 1342, 1625, 1932
HpyF3I CTNAG 9 cut(s) 603, 1025, 1142, 1185, 1232, 1371, 1653, 1794, 1878
HpySE526I ACGT 3 cut(s) 1041, 1070, 1504
Hsp92I GRCGYC 3 cut(s) 171, 1155, 1619
Hsp92II CATG 6 cut(s) 304, 1350, 1406, 1433, 1693, 1832
HspAI GCGC 4 cut(s) 335, 1115, 1619, 1783
KasI GGCGCC 1 cut(s) 1618
KpnI GGTACC 1 cut(s) 1521
LguI GCTCTTC 1 cut(s) 1046
LmnI GCTCC 1 cut(s) 795
Lsp1109I GCAGC 6 cut(s) 3, 793, 858, 1124, 1305, 1653
LweI GCATC 2 cut(s) 1040, 1944
MaeI CTAG 2 cut(s) 654, 1902
MaeII ACGT 3 cut(s) 1041, 1070, 1504
MaeIII GTNAC 4 cut(s) 193, 808, 1151, 1270
MbiI CCGCTC 1 cut(s) 798
MboII GAAGA 5 cut(s) 313, 420, 1063, 1593, 1777
MflI RGATCY 5 cut(s) 120, 754, 1103, 1452, 1695
MhlI GDGCHC 7 cut(s) 54, 558, 632, 689, 1689, 1728, 1884
MlsI TGGCCA 1 cut(s) 252
MluCI AATT 7 cut(s) 21, 512, 822, 1010, 1319, 1365, 1870
MluNI TGGCCA 1 cut(s) 252
Mly113I GGCGCC 1 cut(s) 1619
MlyI GAGTC 9 cut(s) 66, 122, 201, 254, 267, 467, 1322, 1740, 1762
MmeI TCCRAC 5 cut(s) 117, 381, 654, 987, 1498
Mox20I TGGCCA 1 cut(s) 252
MroXI GAANNNNTTC 2 cut(s) 1769, 1854
MscI TGGCCA 1 cut(s) 252
MseI TTAA 5 cut(s) 1322, 1361, 1440, 1869, 1951
MslI CAYNNNNRTG 1 cut(s) 1345
Msp20I TGGCCA 1 cut(s) 252
MspR9I CCNGG 9 cut(s) 118, 133, 311, 383, 566, 626, 689, 752, 1489
MvaI CCWGG 3 cut(s) 133, 566, 626
MvnI CGCG 4 cut(s) 335, 638, 879, 1160
MwoI GCNNNNNNNGC 7 cut(s) 177, 228, 885, 1293, 1342, 1625, 1932
NarI GGCGCC 1 cut(s) 1619
NciI CCSGG 6 cut(s) 118, 311, 383, 689, 752, 1489
NcoI CCATGG 1 cut(s) 1346
NlaIII CATG 6 cut(s) 304, 1350, 1406, 1433, 1693, 1832
NmeAIII GCCGAG 1 cut(s) 493
NmuCI GTSAC 3 cut(s) 193, 808, 1151
PaeR7I CTCGAG 1 cut(s) 1748
PciSI GCTCTTC 1 cut(s) 1046
PcsI WCGNNNNNNNCGW 1 cut(s) 999
PdmI GAANNNNTTC 2 cut(s) 1769, 1854
PfeI GAWTC 6 cut(s) 4, 140, 547, 667, 707, 1561
Pfl23II CGTACG 2 cut(s) 537, 1038
PfoI TCCNGGA 1 cut(s) 381
PleI GAGTC 9 cut(s) 66, 122, 200, 254, 267, 466, 1322, 1740, 1761
PluTI GGCGCC 1 cut(s) 1622
PpsI GAGTC 9 cut(s) 66, 122, 200, 254, 267, 466, 1322, 1740, 1761
Ppu21I YACGTR 2 cut(s) 1042, 1071
Psp124BI GAGCTC 1 cut(s) 1884
Psp6I CCWGG 3 cut(s) 131, 564, 624
PspFI CCCAGC 1 cut(s) 1085
PspGI CCWGG 3 cut(s) 131, 564, 624
PspLI CGTACG 2 cut(s) 537, 1038
PspOMI GGGCCC 1 cut(s) 628
PsrI GAACNNNNNNTAC 2 cut(s) 488, 520
PsuI RGATCY 5 cut(s) 120, 754, 1103, 1452, 1695
RseI CAYNNNNRTG 1 cut(s) 1345
SacI GAGCTC 1 cut(s) 1884
SapI GCTCTTC 1 cut(s) 1046
SaqAI TTAA 5 cut(s) 1322, 1361, 1440, 1869, 1951
SchI GAGTC 9 cut(s) 66, 122, 201, 254, 267, 467, 1322, 1740, 1762
ScrFI CCNGG 9 cut(s) 118, 133, 311, 383, 566, 626, 689, 752, 1489
SduI GDGCHC 7 cut(s) 54, 558, 632, 689, 1689, 1728, 1884
SfaNI GCATC 2 cut(s) 1040, 1944
SfoI GGCGCC 1 cut(s) 1620
Sfr274I CTCGAG 1 cut(s) 1748
SinI GGWCC 5 cut(s) 374, 385, 568, 1210, 1592
SlaI CTCGAG 1 cut(s) 1748
SmiMI CAYNNNNRTG 1 cut(s) 1345
SmlI CTYRAG 3 cut(s) 266, 401, 1748
SmoI CTYRAG 3 cut(s) 266, 401, 1748
SnaBI TACGTA 1 cut(s) 1071
Sse9I AATT 7 cut(s) 21, 512, 822, 1010, 1319, 1365, 1870
SspDI GGCGCC 1 cut(s) 1618
SspMI CTAG 2 cut(s) 654, 1902
SstI GAGCTC 1 cut(s) 1884
StyD4I CCNGG 9 cut(s) 116, 131, 309, 381, 564, 624, 687, 750, 1487
StyI CCWWGG 2 cut(s) 1346, 1901
TaaI ACNGT 4 cut(s) 56, 814, 1316, 1915
TaiI ACGT 3 cut(s) 1044, 1073, 1507
TasI AATT 7 cut(s) 21, 512, 822, 1010, 1319, 1365, 1870
TatI WGTACW 4 cut(s) 1299, 1395, 1668, 1810
TauI GCSGC 5 cut(s) 332, 335, 801, 1160, 1339
TfiI GAWTC 6 cut(s) 4, 140, 547, 667, 707, 1561
Tru1I TTAA 5 cut(s) 1322, 1361, 1440, 1869, 1951
Tru9I TTAA 5 cut(s) 1322, 1361, 1440, 1869, 1951
TscAI CASTG 4 cut(s) 59, 167, 813, 1882
TseFI GTSAC 3 cut(s) 193, 808, 1151
TseI GCWGC 6 cut(s) 16, 781, 871, 1112, 1293, 1641
Tsp45I GTSAC 3 cut(s) 193, 808, 1151
TspDTI ATGAA 5 cut(s) 1270, 1418, 1419, 1817, 1922
TspGWI ACGGA 3 cut(s) 660, 672, 784
TspRI CASTG 4 cut(s) 59, 167, 813, 1882
VneI GTGCAC 1 cut(s) 50
VpaK11BI GGWCC 5 cut(s) 374, 385, 568, 1210, 1592
XapI RAATTY 1 cut(s) 512
XhoI CTCGAG 1 cut(s) 1748
XmaJI CCTAGG 1 cut(s) 1901
XmnI GAANNNNTTC 2 cut(s) 1769, 1854
XspI CTAG 2 cut(s) 654, 1902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.