Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0010580

Physical Location & Seq

Genomic Coordinates Forward (+)

13925182 .. 13928005

Gene Structure 2824 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1093600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1731 bp

ATGGGGAGTGAGATCGTGTCTGAGTCCTGGTTAGGTAGTTTGAGGTTCTCATGGAGTCGTGTGGCGGAGGCTGACAAGCCGGTGATTGGCGTTCTGGCTTTTGAAGTTGCTGGGTTGATGTTAAAGGTGGTTGATTTATGGAATAGTGTGAGTGAGAATGAGATGCTTAGGTTGAGGGAAGAGGTTGTTTACTCTATTGGGGTCAGAAGGCTTGTGTCTGATGACGACTATTACTTGATGGAAATTGCACTGAATGAGATAATTGAGAATTTGGGATATCTGGCAGTGTCTGTTGTCAGGCTTGGGAAGAAGTGCACCGACCCTGTGTATCACTGTTTTGAAGAATTTTTTGATGACCCTGTGGAGAATGGTTTTCAGTGGCTTGGGTGGGAATACAAGTGGAAGAAAATGGAGAGGAAAGTGAAGAAAATGAAGAGATTTATTGAAGCAACAATGCAACTATCCCAGGAGCTGGAAGTGCTGGCGGAGCTTGAGCAAACTCTGAGGAGAATGCGCGCTAATCCTGATGTAAATCGGGTAAAATTGCTCGAGTTTCAACAGAAGGTAATGTGGCGGCGTCAAGAAGTAAAAGGTTTACAAGAGATGTCTCCATGGAGTAGAACATATGATTACACTGTCCGACTTTTGGCAAGATCCCTTTTTACAATGTTAGATAGGATAAAGTTTGTCTTTGGATTTCATCAAATGGCTTTGGGAGAGGGAATGGATAGCTCTGAAGTTATCAATTCTGTTTGTCTTTCTCGCAGTCATTCATTTTCTGCTCTTATGCACTCTTCTGTTCATCCATCTGATGGTAATCTATGTGGGTCTGGACCTCTTGGGAGGTCGCTTGCAAAGACGAGGCTTAATGCTCGCAAGAATAGAACAAACAACCAGCGGCAAGCTCATCACCAGTCATCCATCCAACATGGAAGTTACTCCCAACTAAAACCCAAACGGTTTGCTCATCTTGGTCCGTTTAAAGGATGCATGATGGGTGGAAGTAATTCTCCTGTTTTTGAGAGCTGCAAGCCTGAAATGGGTGGTTCTATGAGGTTGAGAAGTACGGGTATCAAACATTTTGATAGCTTAAAGTACACACATATGGGATCCCAATCTTTCAGTCATGGCATCTATTCTAAACTATCCCTGTTCAATTCAAAATGTACACTACTGAATGCTCCGCCATCTACCCTTGGCGATGCTGCTCTAGCGCTCCATTACGCACATGTGATTGTTTTGATTGAAAAGATAGCTTCATCACCTCACTTGATCAGCCTTGACGAAAGATCTAATCTTTACAACATGTTGACCACAACTATAAGGACTGCTCTGAGAGCTAGACTGAAGTCATATGCCAAAACTACGGGATCATCTGTCTACAATCCTGACCTTGCAGGAGAGTGGAATCTGGCGATGGAGCAGATCTTGGAATGGCTTGCTCCACTAGCCCATAACATGATACGTTGGCATTCTGACCGCAAAATTATGAAGCAGCAGGAAGTTTCCAAGACGAATGTGCTTCTGGTCCAGACCCTCTACTTTGCAAGTCAGGCTAAAACTGAAGCTGCAATTACAGAGCTTCTCATAGGTCTGAACTACATGTGCATGGTTGACGAACTTAATAGGAAGGCTTTGCGAGACGCTGGTGGCAGCCGACCATACGATGATTATATGCTCAAACGGGATGAAATTTCTCAAGAAATTCTGGAGACGGCAATAGCGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3475 DUF668

Protein Analysis

577

Amino Acids

65.7

Weight (kDa)

8.94

Isoelectric Point (pI)

52.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF3475	PF11961	31 - 87	8e-23	Domain of unknown function (DUF3475)
DUF668	PF05003	398 - 489	2.5e-31	Protein of unknown function (DUF668)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010580)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g17590 FvH4_6g17590 FvH4_6g17590
malus_domestica	MD12G1099400.v1.1 MD14G1093600.v1.1
prunus_persica	Prupe.7G119700_v2.0.a1 Prupe.7G119700_v2.0.a1
pyrus_communis	pycom12g09420 pycom12g09430 pycom12g09740
rosa_chinensis	RchiOBHm_Chr3g0471861
rosa_laevigata	RLG00000024123
rosa_multiflora	Rmu_sc0000423.1_g000028
rosa_roxburghii	Rroxscaffold_6G00409470
rosa_rugosa	Rorug03G0120800
rosa_samantha	Rh3AG172100 Rh3BG197500 Rh3CG188500 Rh3CG196100 Rh3DG176800
rosa_wichuraiana	Rw3G016840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	472, 812
AccI	GTMKAC	1 cut(s)	1380
AccII	CGCG	1 cut(s)	516
AciI	CCGC	6 cut(s)	65, 485, 574, 898, 1184, 1480
AclWI	GGATC	4 cut(s)	648, 1104, 1117, 1378
AcsI	RAATTY	4 cut(s)	268, 344, 1692, 1704
AcuI	CTGAAG	3 cut(s)	756, 1367, 1584
AcyI	GRCGYC	1 cut(s)	577
AfaI	GTAC	3 cut(s)	1066, 1097, 1168
AfeI	AGCGCT	1 cut(s)	1215
AfiI	CCNNNNNNNGG	6 cut(s)	86, 472, 646, 812, 983, 1040
AflIII	ACRYGT	3 cut(s)	1228, 1305, 1602
AgsI	TTSAA	7 cut(s)	104, 341, 446, 557, 1156, 1161, 1247
AjnI	CCWGG	2 cut(s)	26, 465
Alw21I	GWGCWC	1 cut(s)	317
Alw26I	GTCTC	3 cut(s)	612, 1635, 1706
Alw44I	GTGCAC	1 cut(s)	313
AlwI	GGATC	4 cut(s)	648, 1104, 1117, 1378
AlwNI	CAGNNNCTG	2 cut(s)	290, 472
Ama87I	CYCGRG	1 cut(s)	548
Aor51HI	AGCGCT	1 cut(s)	1215
ApaLI	GTGCAC	1 cut(s)	313
ApeKI	GCWGC	5 cut(s)	1026, 1205, 1495, 1568, 1653
ApoI	RAATTY	4 cut(s)	268, 344, 1692, 1704
Asp700I	GAANNNNTTC	1 cut(s)	1006
AspLEI	GCGC	3 cut(s)	516, 518, 1216
AspS9I	GGNCC	3 cut(s)	833, 974, 1528
AsuHPI	GGTGA	3 cut(s)	94, 902, 1254
AvaI	CYCGRG	1 cut(s)	548
AvaII	GGWCC	3 cut(s)	833, 974, 1528
BaeGI	GKGCMC	1 cut(s)	317
BamHI	GGATCC	1 cut(s)	1109
Bbv12I	GWGCWC	1 cut(s)	317
BbvI	GCAGC	5 cut(s)	1013, 1192, 1507, 1555, 1665
BccI	CCATC	7 cut(s)	232, 806, 814, 929, 988, 1195, 1411
BciT130I	CCWGG	2 cut(s)	28, 467
BclI	TGATCA	1 cut(s)	1272
BcoDI	GTCTC	3 cut(s)	612, 1635, 1706
BfaI	CTAG	3 cut(s)	1211, 1341, 1448
BfoI	RGCGCY	1 cut(s)	1217
BglII	AGATCT	2 cut(s)	1289, 1425
BisI	GCNGC	7 cut(s)	575, 899, 1027, 1206, 1496, 1569, 1654
BlsI	GCNGC	7 cut(s)	576, 900, 1028, 1207, 1497, 1570, 1655
Bme1390I	CCNGG	2 cut(s)	28, 467
Bme18I	GGWCC	3 cut(s)	833, 974, 1528
BmeT110I	CYCGRG	1 cut(s)	548
BmgT120I	GGNCC	3 cut(s)	833, 974, 1528
BmiI	GGNNCC	1 cut(s)	1111
BmrFI	CCNGG	2 cut(s)	28, 467
BmsI	GCATC	4 cut(s)	153, 977, 1140, 1192
BoxI	GACNNNNGTC	1 cut(s)	1348
BpmI	CTGGAG	1 cut(s)	1730
Bpu10I	CCTNAGC	1 cut(s)	167
BpuEI	CTTGAG	2 cut(s)	512, 1683
BsaBI	GATNNNNATC	3 cut(s)	531, 816, 1114
BsaHI	GRCGYC	1 cut(s)	577
BsaJI	CCNNGG	3 cut(s)	465, 611, 1195
Bsc4I	CCNNNNNNNGG	6 cut(s)	86, 472, 646, 812, 983, 1040
Bse118I	RCCGGY	1 cut(s)	79
Bse1I	ACTGG	1 cut(s)	913
Bse8I	GATNNNNATC	3 cut(s)	531, 816, 1114
BseBI	CCWGG	2 cut(s)	28, 467
BseDI	CCNNGG	3 cut(s)	465, 611, 1195
BseGI	GGATG	5 cut(s)	802, 917, 921, 992, 1693
BseJI	GATNNNNATC	3 cut(s)	531, 816, 1114
BseLI	CCNNNNNNNGG	6 cut(s)	86, 472, 646, 812, 983, 1040
BseMII	CTCAG	3 cut(s)	12, 494, 1325
BseNI	ACTGG	1 cut(s)	913
BsePI	GCGCGC	1 cut(s)	514
BseRI	GAGGAG	1 cut(s)	520
BseSI	GKGCMC	1 cut(s)	317
BseXI	GCAGC	5 cut(s)	1013, 1192, 1507, 1555, 1665
BseYI	CCCAGC	1 cut(s)	110
Bsh1236I	CGCG	1 cut(s)	516
BsiHKAI	GWGCWC	1 cut(s)	317
BsiHKCI	CYCGRG	1 cut(s)	548
BsiSI	CCGG	1 cut(s)	80
BslI	CCNNNNNNNGG	6 cut(s)	86, 472, 646, 812, 983, 1040
BsmAI	GTCTC	3 cut(s)	612, 1635, 1706
BsmBI	CGTCTC	2 cut(s)	1635, 1706
BsmI	GAATGC	3 cut(s)	516, 1183, 1471
BsoBI	CYCGRG	1 cut(s)	548
Bsp1286I	GDGCHC	1 cut(s)	317
Bsp1407I	TGTACA	1 cut(s)	1166
Bsp143I	GATC	7 cut(s)	12, 653, 1109, 1272, 1289, 1370, 1425
Bsp19I	CCATGG	1 cut(s)	611
BspACI	CCGC	6 cut(s)	65, 485, 574, 898, 1184, 1480
BspCNI	CTCAG	3 cut(s)	13, 495, 1326
BspFNI	CGCG	1 cut(s)	516
BspLI	GGNNCC	1 cut(s)	1111
BspPI	GGATC	4 cut(s)	648, 1104, 1117, 1378
BsrFI	RCCGGY	1 cut(s)	79
BsrGI	TGTACA	1 cut(s)	1166
BsrI	ACTGG	1 cut(s)	913
BssAI	RCCGGY	1 cut(s)	79
BssECI	CCNNGG	3 cut(s)	465, 611, 1195
BssHII	GCGCGC	1 cut(s)	514
BssMI	GATC	7 cut(s)	12, 653, 1109, 1272, 1289, 1370, 1425
BssNI	GRCGYC	1 cut(s)	577
BssT1I	CCWWGG	2 cut(s)	611, 1195
Bst2UI	CCWGG	2 cut(s)	28, 467
Bst4CI	ACNGT	3 cut(s)	335, 637, 960
Bst6I	CTCTTC	3 cut(s)	174, 428, 799
BstACI	GRCGYC	1 cut(s)	577
BstAUI	TGTACA	1 cut(s)	1166
BstC8I	GCNNGC	7 cut(s)	483, 516, 852, 874, 903, 1031, 1440
BstDEI	CTNAG	4 cut(s)	21, 167, 503, 1334
BstDSI	CCRYGG	1 cut(s)	611
BstF5I	GGATG	5 cut(s)	802, 917, 921, 992, 1693
BstFNI	CGCG	1 cut(s)	516
BstH2I	RGCGCY	1 cut(s)	1217
BstHHI	GCGC	3 cut(s)	516, 518, 1216
BstKTI	GATC	7 cut(s)	15, 656, 1112, 1275, 1292, 1373, 1428
BstMAI	GTCTC	3 cut(s)	612, 1635, 1706
BstMBI	GATC	7 cut(s)	12, 653, 1109, 1272, 1289, 1370, 1425
BstMWI	GCNNNNNNNGC	6 cut(s)	478, 487, 1211, 1337, 1448, 1553
BstNI	CCWGG	2 cut(s)	28, 467
BstNSI	RCATGY	3 cut(s)	1232, 1309, 1606
BstPAI	GACNNNNGTC	1 cut(s)	1348
BstSCI	CCNGG	2 cut(s)	26, 465
BstSLI	GKGCMC	1 cut(s)	317
BstUI	CGCG	1 cut(s)	516
BstV1I	GCAGC	5 cut(s)	1013, 1192, 1507, 1555, 1665
BstX2I	RGATCY	4 cut(s)	653, 1109, 1289, 1425
BstYI	RGATCY	4 cut(s)	653, 1109, 1289, 1425
BtgI	CCRYGG	1 cut(s)	611
BtgZI	GCGATG	2 cut(s)	1215, 1430
BtsCI	GGATG	5 cut(s)	802, 917, 921, 992, 1693
BtsI	GCAGTG	1 cut(s)	291
BtsIMutI	CAGTG	5 cut(s)	248, 291, 331, 383, 633
Cac8I	GCNNGC	7 cut(s)	483, 516, 852, 874, 903, 1031, 1440
CaiI	CAGNNNCTG	2 cut(s)	290, 472
CfoI	GCGC	3 cut(s)	516, 518, 1216
Cfr10I	RCCGGY	1 cut(s)	79
Cfr13I	GGNCC	3 cut(s)	833, 974, 1528
CseI	GACGC	2 cut(s)	566, 1652
Csp6I	GTAC	3 cut(s)	1065, 1096, 1167
CviQI	GTAC	3 cut(s)	1065, 1096, 1167
DdeI	CTNAG	4 cut(s)	21, 167, 503, 1334
DpnI	GATC	7 cut(s)	14, 655, 1111, 1274, 1291, 1372, 1427
DpnII	GATC	7 cut(s)	12, 653, 1109, 1272, 1289, 1370, 1425
DraI	TTTAAA	1 cut(s)	982
Eam1104I	CTCTTC	3 cut(s)	174, 428, 799
EarI	CTCTTC	3 cut(s)	174, 428, 799
EciI	GGCGGA	3 cut(s)	80, 500, 1173
Eco130I	CCWWGG	2 cut(s)	611, 1195
Eco32I	GATATC	1 cut(s)	278
Eco47I	GGWCC	3 cut(s)	833, 974, 1528
Eco47III	AGCGCT	1 cut(s)	1215
Eco57I	CTGAAG	3 cut(s)	756, 1367, 1584
Eco88I	CYCGRG	1 cut(s)	548
EcoRII	CCWGG	2 cut(s)	26, 465
EcoRV	GATATC	1 cut(s)	278
EcoT14I	CCWWGG	2 cut(s)	611, 1195
EcoT22I	ATGCAT	1 cut(s)	992
ErhI	CCWWGG	2 cut(s)	611, 1195
Esp3I	CGTCTC	2 cut(s)	1635, 1706
FalI	AAGNNNNNCTT	2 cut(s)	674, 706
FauNDI	CATATG	3 cut(s)	625, 1104, 1354
FbaI	TGATCA	1 cut(s)	1272
FblI	GTMKAC	1 cut(s)	1380
Fnu4HI	GCNGC	7 cut(s)	575, 899, 1027, 1206, 1496, 1569, 1654
FokI	GGATG	5 cut(s)	789, 904, 908, 999, 1700
Fsp4HI	GCNGC	7 cut(s)	575, 899, 1027, 1206, 1496, 1569, 1654
FspBI	CTAG	3 cut(s)	1211, 1341, 1448
GlaI	GCGC	3 cut(s)	515, 517, 1215
GluI	GCNGC	7 cut(s)	575, 899, 1027, 1206, 1496, 1569, 1654
GsaI	CCCAGC	1 cut(s)	114
GsuI	CTGGAG	1 cut(s)	1730
HaeII	RGCGCY	1 cut(s)	1217
HapII	CCGG	1 cut(s)	80
HgaI	GACGC	2 cut(s)	566, 1652
HhaI	GCGC	3 cut(s)	516, 518, 1216
Hin1I	GRCGYC	1 cut(s)	577
Hin6I	GCGC	3 cut(s)	514, 516, 1214
HinP1I	GCGC	3 cut(s)	514, 516, 1214
HincII	GTYRAC	2 cut(s)	1311, 1615
HindII	GTYRAC	2 cut(s)	1311, 1615
HinfI	GANTC	3 cut(s)	23, 55, 1408
HpaII	CCGG	1 cut(s)	80
HphI	GGTGA	3 cut(s)	94, 902, 1254
Hpy166II	GTNNAC	8 cut(s)	190, 315, 596, 1098, 1169, 1311, 1381, 1615
Hpy188III	TCNNGA	7 cut(s)	524, 581, 831, 1388, 1531, 1700, 1709
Hpy8I	GTNNAC	8 cut(s)	190, 315, 596, 1098, 1169, 1311, 1381, 1615
HpyAV	CCTTC	3 cut(s)	201, 556, 1624
HpyCH4III	ACNGT	3 cut(s)	335, 637, 960
HpyCH4IV	ACGT	1 cut(s)	1465
HpyF10VI	GCNNNNNNNGC	6 cut(s)	478, 487, 1211, 1337, 1448, 1553
HpyF3I	CTNAG	4 cut(s)	21, 167, 503, 1334
HpySE526I	ACGT	1 cut(s)	1465
Hsp92I	GRCGYC	1 cut(s)	577
HspAI	GCGC	3 cut(s)	514, 516, 1214
Ksp22I	TGATCA	1 cut(s)	1272
Kzo9I	GATC	7 cut(s)	12, 653, 1109, 1272, 1289, 1370, 1425
LmnI	GCTCC	6 cut(s)	469, 487, 1186, 1221, 1420, 1447
Lsp1109I	GCAGC	5 cut(s)	1013, 1192, 1507, 1555, 1665
LweI	GCATC	4 cut(s)	153, 977, 1140, 1192
MaeI	CTAG	3 cut(s)	1211, 1341, 1448
MaeII	ACGT	1 cut(s)	1465
MaeIII	GTNAC	1 cut(s)	935
MalI	GATC	7 cut(s)	14, 655, 1111, 1274, 1291, 1372, 1427
MboI	GATC	7 cut(s)	12, 653, 1109, 1272, 1289, 1370, 1425
MboII	GAAGA	7 cut(s)	191, 319, 353, 415, 436, 445, 786
MflI	RGATCY	4 cut(s)	653, 1109, 1289, 1425
MhlI	GDGCHC	1 cut(s)	317
MlyI	GAGTC	2 cut(s)	32, 64
MmeI	TCCRAC	2 cut(s)	664, 949
Mph1103I	ATGCAT	1 cut(s)	992
MroXI	GAANNNNTTC	1 cut(s)	1006
MseI	TTAA	5 cut(s)	122, 867, 981, 1091, 1623
MslI	CAYNNNNRTG	2 cut(s)	1103, 1607
MspA1I	CMGCKG	1 cut(s)	898
MspI	CCGG	1 cut(s)	80
MspR9I	CCNGG	2 cut(s)	28, 467
Mva1269I	GAATGC	3 cut(s)	516, 1183, 1471
MvaI	CCWGG	2 cut(s)	28, 467
MvnI	CGCG	1 cut(s)	516
MwoI	GCNNNNNNNGC	6 cut(s)	478, 487, 1211, 1337, 1448, 1553
NcoI	CCATGG	1 cut(s)	611
NdeI	CATATG	3 cut(s)	625, 1104, 1354
NdeII	GATC	7 cut(s)	12, 653, 1109, 1272, 1289, 1370, 1425
NlaIV	GGNNCC	1 cut(s)	1111
NsiI	ATGCAT	1 cut(s)	992
NspI	RCATGY	3 cut(s)	1232, 1309, 1606
PaeR7I	CTCGAG	1 cut(s)	548
PauI	GCGCGC	1 cut(s)	514
PciI	ACATGT	3 cut(s)	1228, 1305, 1602
PcsI	WCGNNNNNNNCGW	1 cut(s)	1721
PctI	GAATGC	3 cut(s)	516, 1183, 1471
PdmI	GAANNNNTTC	1 cut(s)	1006
PfeI	GAWTC	1 cut(s)	1408
PflMI	CCANNNNNTGG	2 cut(s)	472, 812
PkrI	GCNGC	7 cut(s)	576, 900, 1028, 1207, 1497, 1570, 1655
PleI	GAGTC	2 cut(s)	31, 63
PpsI	GAGTC	2 cut(s)	31, 63
PscI	ACATGT	3 cut(s)	1228, 1305, 1602
PshAI	GACNNNNGTC	1 cut(s)	1348
Psp6I	CCWGG	2 cut(s)	26, 465
PspFI	CCCAGC	1 cut(s)	110
PspGI	CCWGG	2 cut(s)	26, 465
PspN4I	GGNNCC	1 cut(s)	1111
PspPI	GGNCC	3 cut(s)	833, 974, 1528
PspXI	VCTCGAGB	1 cut(s)	548
PstNI	CAGNNNCTG	2 cut(s)	290, 472
PsuI	RGATCY	4 cut(s)	653, 1109, 1289, 1425
PteI	GCGCGC	1 cut(s)	514
RsaI	GTAC	3 cut(s)	1066, 1097, 1168
RsaNI	GTAC	3 cut(s)	1065, 1096, 1167
RseI	CAYNNNNRTG	2 cut(s)	1103, 1607
SaqAI	TTAA	5 cut(s)	122, 867, 981, 1091, 1623
SatI	GCNGC	7 cut(s)	575, 899, 1027, 1206, 1496, 1569, 1654
Sau3AI	GATC	7 cut(s)	12, 653, 1109, 1272, 1289, 1370, 1425
Sau96I	GGNCC	3 cut(s)	833, 974, 1528
SchI	GAGTC	2 cut(s)	32, 64
ScrFI	CCNGG	2 cut(s)	28, 467
SduI	GDGCHC	1 cut(s)	317
SfaNI	GCATC	4 cut(s)	153, 977, 1140, 1192
Sfr274I	CTCGAG	1 cut(s)	548
SinI	GGWCC	3 cut(s)	833, 974, 1528
SlaI	CTCGAG	1 cut(s)	548
SmiMI	CAYNNNNRTG	2 cut(s)	1103, 1607
SmlI	CTYRAG	3 cut(s)	491, 548, 1698
SmoI	CTYRAG	3 cut(s)	491, 548, 1698
SsiI	CCGC	6 cut(s)	65, 485, 574, 898, 1184, 1480
SspMI	CTAG	3 cut(s)	1211, 1341, 1448
StyD4I	CCNGG	2 cut(s)	26, 465
StyI	CCWWGG	2 cut(s)	611, 1195
TaaI	ACNGT	3 cut(s)	335, 637, 960
TaiI	ACGT	1 cut(s)	1468
TaqI	TCGA	1 cut(s)	549
TatI	WGTACW	2 cut(s)	1095, 1166
TauI	GCSGC	2 cut(s)	577, 901
TfiI	GAWTC	1 cut(s)	1408
Tru1I	TTAA	5 cut(s)	122, 867, 981, 1091, 1623
Tru9I	TTAA	5 cut(s)	122, 867, 981, 1091, 1623
TscAI	CASTG	5 cut(s)	255, 291, 338, 383, 640
TseI	GCWGC	5 cut(s)	1026, 1205, 1495, 1568, 1653
TspDTI	ATGAA	7 cut(s)	446, 689, 762, 791, 1248, 1505, 1704
TspGWI	ACGGA	1 cut(s)	966
TspRI	CASTG	5 cut(s)	255, 291, 338, 383, 640
Van91I	CCANNNNNTGG	2 cut(s)	472, 812
VneI	GTGCAC	1 cut(s)	313
VpaK11BI	GGWCC	3 cut(s)	833, 974, 1528
XapI	RAATTY	4 cut(s)	268, 344, 1692, 1704
XceI	RCATGY	3 cut(s)	1232, 1309, 1606
XhoI	CTCGAG	1 cut(s)	548
XmiI	GTMKAC	1 cut(s)	1380
XmnI	GAANNNNTTC	1 cut(s)	1006
XspI	CTAG	3 cut(s)	1211, 1341, 1448
Zsp2I	ATGCAT	1 cut(s)	992

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.