MD14G1116300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009121
Physical Location & Seq
Reverse (-)
18867298 .. 18871069
3772 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1116300.v1.1.491

Sequence Viewer

Length: 2007 bp
ATGGACAATAGGTTGCAGAGTCTGAGTTTCGCTGCAAATTTTTCTTCAAATGCCTTCAAGATCTTGGGCAACTCCATGCAAGTTGGTGGAGCCGGAGGGGAGTATTCTGCAGATACTATCTTACGGCTTAATTCCCCTGGTTCTTCAATGCCTTACATGCCAAGCCCAAAAGGAATCAAACGGAAGTGGAGTTTGGTTGGTGGATCTTTGACCCAGCACGTTAGCTCTTCACTGGTGCTTGGGCTTGGCCGTTCAACGAGTTCCTCAGACAGCAAGGGGAGCTCAGCTACTGCTTGCACTACAATGTCTTCAACCAAAGAAATGGATGAGGAGTCCTCGATGGATTTTGAATTGGACTTTGCTCTTCATCTTGGAATTGAAAAGGTACCTAGCCCGAGGAAACCTGCTAAGAGTGCACTGGAACTGCAGCCCAAGGTTGACTTGGAGTTAAGTCTCTCCACTGGGTTGTCTGAATCTGAAATCGCTAGTGTCAATCCAAGCCCCGGCTCGGTCCTTTCAGGCATGGAGTTGCCATTGGCGACTGGTAGGGCCCAAAATGTAGATGGAGTAACTTCATTCCCTTTGAAGCGAAGGTTTTCCATGCAACCATTGCAGACTTCATTCAACCCAGGGGCTAGCGTCCTTTTCAATGAGGTTTCACAAAAGATTGACTTTACTGCTAGTGTTCCGGATCTCTCATCAAGCATGTTAACCACTCCAAATAGCTCAGTCACCTGTATTTCTTCTGGGATAACACAGAAACAACAGTCACAGCATCGCAGCTCTAATTCAAAGACATGCCAGGTTGAAGGGTGTGGAAAGGGTGCCAGAGGTGCCTCAGGCCGTTGCATATCTCATGGTGGTGGTAGGAGGTGTCAGAGATCTGGCTGCCACAAGGGTGCTGAAGGCAGGACAGTGTACTGCAAGGCCCACGGTGGTGGCCGGAGATGTGAATTCCTTGGGTGCACAAAGAGTGCCGAAGGACGTACAGACTTTTGTATTGCTCACGGTGGTGGCCGGAGGTGCAGTCATGAAGGTTGCAGTCGAGCTGCCAGAGGGAAATCAGGATTGTGCATCAGGCATGGTGGTGGCAAGAGATGTCAAAGAGAAAATTGCACAAAGAGTGCGGAGGGCCTTTCGGGCCTTTGCATCTCACATGGAGGAGGCCGTCGATGCCAAACTATAGGATGCACAAAAGGGGCGCAAGGGAGTACAATGTTCTGCAAGGCTCATGGTGGGGGGAAGCGGTGCACTGCTCCTGGTTGCACCAAGGGAGCTGAAGGGAGCACACCCTATTGCAAAGGCCATGGTGGAGGGAAAAGGTGTGCATTCCAAGGTGGTGGGCATTGTACAAAGAGTGTGCATGGTGGTACCAACTTCTGTGTGGCACATGGGGGTGGCAAGCGATGTGCCATGCCTGAGTGCACAAAGAGTGCGAGGGGACGAACTGACTACTGTGTCCGACATGGTGGGGGAAAGAGGTGCAAGTCTGAAGGGTGTGGTAAGAGTGCGCAAGGCAGCACTGACTTCTGCAAGGCTCATGGTGGAGGGAAGAGATGCTCATGGGGCCATCCGGGGTCAGTTTATGGTGGCCAAGCTAGCGCCCCTTGTAACTCATTTGCAAGGGGGAAGACAGGCCTCTGTGCCCTCCATAGTGGTCTGGTTCAAGATAACAGGGTTCATGGAGGTATTACTTTGGGACCTATGGTTCAAGACCCTAAACTTGGGAAGTCGGAGAAGATGAAAGAAGTAGTCATTGCTGACGACATGAATGTTGATGTGATGAATATCGTGAGTGGCATAAGGACTTCAGCAACTGGAGCTTATTCTGACTTGAAACATGGTGGTGAATCTTCAGTCCCCACGCTCGTGCCAGAAGGCAGGGTGCATGGGGGAAGTCTGATGGCTCTGTTGGCGGGTGACGGTTCAGATTCCAACGCAAACAGCATGGGAAGCAATCCATCTGGGCCAAAGAAGTCCTACATGATGCATCTGAACTGGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

669

Amino Acids

69.27

Weight (kDa)

9.36

Isoelectric Point (pI)

45.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zf_WRKY19 PF24906 314 - 338 1.1e-09 WRKY19 zinc finger domain
Zf_WRKY19 PF24906 339 - 363 1.9e-08 WRKY19 zinc finger domain
Zf_WRKY19 PF24906 364 - 388 3.3e-10 WRKY19 zinc finger domain
Zf_WRKY19 PF24906 389 - 413 5.3e-07 WRKY19 zinc finger domain
Zf_WRKY19 PF24906 414 - 438 4.7e-06 WRKY19 zinc finger domain
Zf_WRKY19 PF24906 467 - 491 3.6e-10 WRKY19 zinc finger domain
Zf_WRKY19 PF24906 492 - 516 2e-07 WRKY19 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1457
Acc16I TGCGCA 1 cut(s) 1512
Acc36I ACCTGC 1 cut(s) 412
Acc65I GGTACC 2 cut(s) 385, 1370
AccB1I GGYRCC 4 cut(s) 385, 824, 833, 1370
AccIII TCCGGA 1 cut(s) 688
AciI CCGC 3 cut(s) 1127, 1246, 1916
AclWI GGATC 2 cut(s) 211, 699
AcoI YGGCCR 4 cut(s) 247, 940, 1015, 1591
AcsI RAATTY 2 cut(s) 37, 953
AcuI CTGAAG 5 cut(s) 924, 1299, 1512, 1794, 1839
AfaI GTAC 6 cut(s) 387, 920, 988, 1213, 1351, 1372
AfiI CCNNNNNNNGG 3 cut(s) 503, 508, 1724
AjnI CCWGG 4 cut(s) 136, 628, 801, 1258
AjuI GAANNNNNNNTTGG 2 cut(s) 176, 208
AleI CACNNNNGTG 4 cut(s) 897, 936, 1011, 1868
AloI GAACNNNNNNTCC 2 cut(s) 1692, 1724
AluBI AGCT 9 cut(s) 225, 282, 287, 726, 783, 1049, 1277, 1598, 1823
AluI AGCT 9 cut(s) 225, 282, 287, 726, 783, 1049, 1277, 1598, 1823
Alw21I GWGCWC 6 cut(s) 284, 418, 968, 1253, 1289, 1427
Alw26I GTCTC 1 cut(s) 458
Alw44I GTGCAC 4 cut(s) 414, 964, 1249, 1423
AlwI GGATC 2 cut(s) 211, 699
AlwNI CAGNNNCTG 3 cut(s) 22, 290, 1817
Ama87I CYCGRG 1 cut(s) 394
Aor13HI TCCGGA 1 cut(s) 688
ApaI GGGCCC 1 cut(s) 553
ApaLI GTGCAC 4 cut(s) 414, 964, 1249, 1423
ApeKI GCWGC 6 cut(s) 32, 427, 780, 888, 1049, 1518
ApoI RAATTY 2 cut(s) 37, 953
Asp700I GAANNNNTTC 1 cut(s) 595
Asp718I GGTACC 2 cut(s) 385, 1370
AspLEI GCGC 3 cut(s) 1204, 1513, 1604
AspS9I GGNCC 9 cut(s) 511, 549, 550, 928, 1132, 1141, 1567, 1700, 1967
AsuC2I CCSGG 2 cut(s) 504, 1575
AsuHPI GGTGA 3 cut(s) 724, 1859, 1931
AsuNHI GCTAGC 2 cut(s) 635, 1598
AvaI CYCGRG 1 cut(s) 394
AvaII GGWCC 2 cut(s) 511, 1700
AxyI CCTNAGG 1 cut(s) 838
BaeGI GKGCMC 6 cut(s) 418, 553, 968, 1253, 1427, 1648
BalI TGGCCA 1 cut(s) 1593
BanI GGYRCC 4 cut(s) 385, 824, 833, 1370
BanII GRGCYC 2 cut(s) 284, 553
BauI CACGAG 1 cut(s) 1868
BbsI GAAGAC 2 cut(s) 300, 1637
Bbv12I GWGCWC 6 cut(s) 284, 418, 968, 1253, 1289, 1427
BbvI GCAGC 6 cut(s) 19, 439, 792, 875, 1036, 1530
BccI CCATC 5 cut(s) 334, 557, 1578, 1897, 1969
BceAI ACGGC 4 cut(s) 140, 234, 828, 1152
BciT130I CCWGG 4 cut(s) 138, 630, 803, 1260
BcnI CCSGG 2 cut(s) 504, 1575
BcoDI GTCTC 1 cut(s) 458
BfaI CTAG 5 cut(s) 390, 486, 636, 681, 1599
BfmI CTRYAG 3 cut(s) 108, 425, 1182
BfoI RGCGCY 1 cut(s) 1605
BfuAI ACCTGC 1 cut(s) 412
BglI GCCNNNNNGGC 1 cut(s) 1140
BglII AGATCT 2 cut(s) 60, 881
BisI GCNGC 6 cut(s) 33, 428, 781, 889, 1050, 1519
BlpI GCTNAGC 1 cut(s) 283
BlsI GCNGC 6 cut(s) 34, 429, 782, 890, 1051, 1520
Bme1390I CCNGG 6 cut(s) 138, 504, 630, 803, 1260, 1575
Bme18I GGWCC 2 cut(s) 511, 1700
BmeT110I CYCGRG 1 cut(s) 394
BmgT120I GGNCC 9 cut(s) 511, 549, 550, 928, 1132, 1141, 1567, 1700, 1967
BmiI GGNNCC 8 cut(s) 91, 387, 551, 826, 835, 1372, 1568, 1701
BmrFI CCNGG 6 cut(s) 138, 504, 630, 803, 1260, 1575
BmrI ACTGGG 1 cut(s) 471
BmsI GCATC 8 cut(s) 784, 1083, 1158, 1163, 1178, 1547, 1977, 1999
BmtI GCTAGC 2 cut(s) 639, 1602
BmuI ACTGGG 1 cut(s) 471
BpiI GAAGAC 2 cut(s) 300, 1637
BplI GAGNNNNNCTC 2 cut(s) 320, 352
BpmI CTGGAG 1 cut(s) 1839
Bpu1102I GCTNAGC 1 cut(s) 283
BpuMI CCSGG 2 cut(s) 504, 1575
BsaBI GATNNNNATC 1 cut(s) 1787
BsaWI WCCGGW 1 cut(s) 688
BsaXI ACNNNNNCTCC 2 cut(s) 1012, 1042
Bsc4I CCNNNNNNNGG 3 cut(s) 503, 508, 1724
Bse1I ACTGG 6 cut(s) 237, 423, 466, 547, 1822, 2003
Bse21I CCTNAGG 1 cut(s) 838
Bse3DI GCAATG 2 cut(s) 608, 1755
Bse8I GATNNNNATC 1 cut(s) 1787
BseAI TCCGGA 1 cut(s) 688
BseBI CCWGG 4 cut(s) 138, 630, 803, 1260
BseGI GGATG 4 cut(s) 331, 1193, 1570, 2007
BseJI GATNNNNATC 1 cut(s) 1787
BseLI CCNNNNNNNGG 3 cut(s) 503, 508, 1724
BseMI GCAATG 2 cut(s) 608, 1755
BseMII CTCAG 6 cut(s) 14, 279, 297, 741, 852, 1410
BseNI ACTGG 6 cut(s) 237, 423, 466, 547, 1822, 2003
BseRI GAGGAG 2 cut(s) 344, 1176
BseSI GKGCMC 6 cut(s) 418, 553, 968, 1253, 1427, 1648
BseXI GCAGC 6 cut(s) 19, 439, 792, 875, 1036, 1530
BseYI CCCAGC 1 cut(s) 213
BsgI GTGCAG 1 cut(s) 1045
BshNI GGYRCC 4 cut(s) 385, 824, 833, 1370
BsiHKAI GWGCWC 6 cut(s) 284, 418, 968, 1253, 1289, 1427
BsiHKCI CYCGRG 1 cut(s) 394
BsiSI CCGG 6 cut(s) 93, 504, 689, 943, 1018, 1574
BslFI GGGAC 3 cut(s) 1455, 1713, 1844
BslI CCNNNNNNNGG 3 cut(s) 503, 508, 1724
BsmAI GTCTC 1 cut(s) 458
BsmFI GGGAC 3 cut(s) 1455, 1713, 1844
BsmI GAATGC 1 cut(s) 1328
BsoBI CYCGRG 1 cut(s) 394
Bsp120I GGGCCC 1 cut(s) 549
Bsp1286I GDGCHC 8 cut(s) 284, 418, 553, 968, 1253, 1289, 1427, 1648
Bsp13I TCCGGA 1 cut(s) 688
Bsp1407I TGTACA 1 cut(s) 1349
Bsp143I GATC 4 cut(s) 60, 203, 691, 881
Bsp1720I GCTNAGC 1 cut(s) 283
Bsp19I CCATGG 1 cut(s) 1306
BspACI CCGC 3 cut(s) 1127, 1246, 1916
BspCNI CTCAG 6 cut(s) 15, 278, 296, 740, 851, 1411
BspEI TCCGGA 1 cut(s) 688
BspHI TCATGA 1 cut(s) 1030
BspLI GGNNCC 8 cut(s) 91, 387, 551, 826, 835, 1372, 1568, 1701
BspMAI CTGCAG 2 cut(s) 112, 429
BspMI ACCTGC 1 cut(s) 412
BspOI GCTAGC 2 cut(s) 639, 1602
BspPI GGATC 2 cut(s) 211, 699
BspQI GCTCTTC 2 cut(s) 232, 369
BspT107I GGYRCC 4 cut(s) 385, 824, 833, 1370
BsrDI GCAATG 2 cut(s) 608, 1755
BsrGI TGTACA 1 cut(s) 1349
BsrI ACTGG 6 cut(s) 237, 423, 466, 547, 1822, 2003
BssMI GATC 4 cut(s) 60, 203, 691, 881
BssSI CACGAG 1 cut(s) 1868
BssT1I CCWWGG 5 cut(s) 432, 958, 1269, 1306, 1333
Bst2BI CACGAG 1 cut(s) 1868
Bst2UI CCWGG 4 cut(s) 138, 630, 803, 1260
Bst4CI ACNGT 6 cut(s) 768, 916, 935, 1010, 1457, 1925
Bst6I CTCTTC 3 cut(s) 232, 369, 1547
BstAPI GCANNNNNTGC 1 cut(s) 610
BstAUI TGTACA 1 cut(s) 1349
BstC8I GCNNGC 4 cut(s) 295, 637, 1403, 1600
BstDEI CTNAG 7 cut(s) 23, 265, 283, 408, 727, 838, 1419
BstDSI CCRYGG 2 cut(s) 931, 1306
BstF5I GGATG 4 cut(s) 331, 1193, 1570, 2007
BstH2I RGCGCY 1 cut(s) 1605
BstHHI GCGC 3 cut(s) 1204, 1513, 1604
BstKTI GATC 4 cut(s) 63, 206, 694, 884
BstMAI GTCTC 1 cut(s) 458
BstMBI GATC 4 cut(s) 60, 203, 691, 881
BstNI CCWGG 4 cut(s) 138, 630, 803, 1260
BstNSI RCATGY 3 cut(s) 160, 709, 801
BstSCI CCNGG 6 cut(s) 136, 502, 628, 801, 1258, 1573
BstSFI CTRYAG 3 cut(s) 108, 425, 1182
BstSLI GKGCMC 6 cut(s) 418, 553, 968, 1253, 1427, 1648
BstV1I GCAGC 6 cut(s) 19, 439, 792, 875, 1036, 1530
BstV2I GAAGAC 2 cut(s) 300, 1637
BstX2I RGATCY 4 cut(s) 60, 203, 691, 881
BstXI CCANNNNNNTGG 3 cut(s) 322, 938, 1340
BstYI RGATCY 4 cut(s) 60, 203, 691, 881
Bsu36I CCTNAGG 1 cut(s) 838
BtgI CCRYGG 2 cut(s) 931, 1306
BtgZI GCGATG 2 cut(s) 761, 1420
BtsCI GGATG 4 cut(s) 331, 1193, 1570, 2007
BtsI GCAGTG 1 cut(s) 1251
BtsIMutI CAGTG 6 cut(s) 230, 416, 459, 921, 1251, 1521
BveI ACCTGC 1 cut(s) 412
Cac8I GCNNGC 4 cut(s) 295, 637, 1403, 1600
CaiI CAGNNNCTG 3 cut(s) 22, 290, 1817
CciI TCATGA 1 cut(s) 1030
CfoI GCGC 3 cut(s) 1204, 1513, 1604
Cfr13I GGNCC 9 cut(s) 511, 549, 550, 928, 1132, 1141, 1567, 1700, 1967
CseI GACGC 1 cut(s) 628
Csp6I GTAC 6 cut(s) 386, 919, 987, 1212, 1350, 1371
CviQI GTAC 6 cut(s) 386, 919, 987, 1212, 1350, 1371
DdeI CTNAG 7 cut(s) 23, 265, 283, 408, 727, 838, 1419
DpnI GATC 4 cut(s) 62, 205, 693, 883
DpnII GATC 4 cut(s) 60, 203, 691, 881
DrdI GACNNNNNNGTC 1 cut(s) 1457
DseDI GACNNNNNNGTC 1 cut(s) 1457
EaeI YGGCCR 4 cut(s) 247, 940, 1015, 1591
Eam1104I CTCTTC 3 cut(s) 232, 369, 1547
EarI CTCTTC 3 cut(s) 232, 369, 1547
Ecl136II GAGCTC 1 cut(s) 282
Eco130I CCWWGG 5 cut(s) 432, 958, 1269, 1306, 1333
Eco147I AGGCCT 1 cut(s) 1638
Eco24I GRGCYC 2 cut(s) 284, 553
Eco47I GGWCC 2 cut(s) 511, 1700
Eco53kI GAGCTC 1 cut(s) 282
Eco57I CTGAAG 5 cut(s) 924, 1299, 1512, 1794, 1839
Eco81I CCTNAGG 1 cut(s) 838
Eco88I CYCGRG 1 cut(s) 394
EcoICRI GAGCTC 1 cut(s) 282
EcoO109I RGGNCCY 3 cut(s) 549, 1132, 1700
EcoRI GAATTC 1 cut(s) 953
EcoRII CCWGG 4 cut(s) 136, 628, 801, 1258
EcoT14I CCWWGG 5 cut(s) 432, 958, 1269, 1306, 1333
EcoT22I ATGCAT 1 cut(s) 1992
EcoT38I GRGCYC 2 cut(s) 284, 553
ErhI CCWWGG 5 cut(s) 432, 958, 1269, 1306, 1333
FalI AAGNNNNNCTT 4 cut(s) 425, 457, 656, 688
FaqI GGGAC 3 cut(s) 1455, 1713, 1844
FauI CCCGC 1 cut(s) 1909
Fnu4HI GCNGC 6 cut(s) 33, 428, 781, 889, 1050, 1519
FokI GGATG 3 cut(s) 338, 1200, 1557
FriOI GRGCYC 2 cut(s) 284, 553
Fsp4HI GCNGC 6 cut(s) 33, 428, 781, 889, 1050, 1519
FspBI CTAG 5 cut(s) 390, 486, 636, 681, 1599
FspI TGCGCA 1 cut(s) 1512
GlaI GCGC 3 cut(s) 1203, 1512, 1603
GluI GCNGC 6 cut(s) 33, 428, 781, 889, 1050, 1519
GsaI CCCAGC 1 cut(s) 217
GsuI CTGGAG 1 cut(s) 1839
HaeII RGCGCY 1 cut(s) 1605
HapII CCGG 6 cut(s) 93, 504, 689, 943, 1018, 1574
HgaI GACGC 1 cut(s) 628
HhaI GCGC 3 cut(s) 1204, 1513, 1604
Hin6I GCGC 3 cut(s) 1202, 1511, 1602
HinP1I GCGC 3 cut(s) 1202, 1511, 1602
HincII GTYRAC 2 cut(s) 439, 711
HindII GTYRAC 2 cut(s) 439, 711
HinfI GANTC 6 cut(s) 19, 174, 332, 473, 1850, 1931
HpaI GTTAAC 1 cut(s) 711
HpaII CCGG 6 cut(s) 93, 504, 689, 943, 1018, 1574
HphI GGTGA 3 cut(s) 724, 1859, 1931
Hpy166II GTNNAC 7 cut(s) 416, 439, 711, 919, 966, 1251, 1425
Hpy188III TCNNGA 7 cut(s) 58, 689, 1031, 1065, 1667, 1712, 1792
Hpy8I GTNNAC 7 cut(s) 416, 439, 711, 919, 966, 1251, 1425
Hpy99I CGWCG 1 cut(s) 1173
HpyAV CCTTC 9 cut(s) 64, 585, 803, 899, 974, 1028, 1274, 1487, 1871
HpyCH4III ACNGT 6 cut(s) 768, 916, 935, 1010, 1457, 1925
HpyCH4IV ACGT 2 cut(s) 219, 985
HpyF3I CTNAG 7 cut(s) 23, 265, 283, 408, 727, 838, 1419
HpySE526I ACGT 2 cut(s) 219, 985
HspAI GCGC 3 cut(s) 1202, 1511, 1602
Kpn2I TCCGGA 1 cut(s) 688
KpnI GGTACC 2 cut(s) 389, 1374
KspAI GTTAAC 1 cut(s) 711
Kzo9I GATC 4 cut(s) 60, 203, 691, 881
LguI GCTCTTC 2 cut(s) 232, 369
LmnI GCTCC 6 cut(s) 89, 279, 1261, 1274, 1284, 1820
Lsp1109I GCAGC 6 cut(s) 19, 439, 792, 875, 1036, 1530
LweI GCATC 8 cut(s) 784, 1083, 1158, 1163, 1178, 1547, 1977, 1999
MaeI CTAG 5 cut(s) 390, 486, 636, 681, 1599
MaeII ACGT 2 cut(s) 219, 985
MaeIII GTNAC 5 cut(s) 568, 730, 768, 1610, 1919
MalI GATC 4 cut(s) 62, 205, 693, 883
MboI GATC 4 cut(s) 60, 203, 691, 881
MflI RGATCY 4 cut(s) 60, 203, 691, 881
MhlI GDGCHC 8 cut(s) 284, 418, 553, 968, 1253, 1289, 1427, 1648
MlsI TGGCCA 1 cut(s) 1593
MluCI AATT 7 cut(s) 37, 130, 350, 375, 787, 953, 1111
MluNI TGGCCA 1 cut(s) 1593
MlyI GAGTC 2 cut(s) 28, 341
MmeI TCCRAC 3 cut(s) 1486, 1713, 1959
Mox20I TGGCCA 1 cut(s) 1593
Mph1103I ATGCAT 1 cut(s) 1992
MroI TCCGGA 1 cut(s) 688
MroXI GAANNNNTTC 1 cut(s) 595
MscI TGGCCA 1 cut(s) 1593
MseI TTAA 3 cut(s) 129, 449, 710
MslI CAYNNNNRTG 9 cut(s) 302, 861, 897, 936, 1011, 1086, 1395, 1845, 1868
Msp20I TGGCCA 1 cut(s) 1593
MspI CCGG 6 cut(s) 93, 504, 689, 943, 1018, 1574
MspR9I CCNGG 6 cut(s) 138, 504, 630, 803, 1260, 1575
Mva1269I GAATGC 1 cut(s) 1328
MvaI CCWGG 4 cut(s) 138, 630, 803, 1260
NciI CCSGG 2 cut(s) 504, 1575
NcoI CCATGG 1 cut(s) 1306
NdeII GATC 4 cut(s) 60, 203, 691, 881
NheI GCTAGC 2 cut(s) 635, 1598
NlaIV GGNNCC 8 cut(s) 91, 387, 551, 826, 835, 1372, 1568, 1701
NmuCI GTSAC 3 cut(s) 730, 768, 1919
NsbI TGCGCA 1 cut(s) 1512
NsiI ATGCAT 1 cut(s) 1992
NspI RCATGY 3 cut(s) 160, 709, 801
OliI CACNNNNGTG 4 cut(s) 897, 936, 1011, 1868
PagI TCATGA 1 cut(s) 1030
PasI CCCWGGG 1 cut(s) 629
PceI AGGCCT 1 cut(s) 1638
PciSI GCTCTTC 2 cut(s) 232, 369
PctI GAATGC 1 cut(s) 1328
PdmI GAANNNNTTC 1 cut(s) 595
PfeI GAWTC 4 cut(s) 174, 473, 1850, 1931
PkrI GCNGC 6 cut(s) 34, 429, 782, 890, 1051, 1520
PleI GAGTC 2 cut(s) 27, 340
PpsI GAGTC 2 cut(s) 27, 340
PpuMI RGGWCCY 1 cut(s) 1700
Psp124BI GAGCTC 1 cut(s) 284
Psp5II RGGWCCY 1 cut(s) 1700
Psp6I CCWGG 4 cut(s) 136, 628, 801, 1258
PspFI CCCAGC 1 cut(s) 213
PspGI CCWGG 4 cut(s) 136, 628, 801, 1258
PspN4I GGNNCC 8 cut(s) 91, 387, 551, 826, 835, 1372, 1568, 1701
PspOMI GGGCCC 1 cut(s) 549
PspPI GGNCC 9 cut(s) 511, 549, 550, 928, 1132, 1141, 1567, 1700, 1967
PspPPI RGGWCCY 1 cut(s) 1700
PstI CTGCAG 2 cut(s) 112, 429
PstNI CAGNNNCTG 3 cut(s) 22, 290, 1817
PsuI RGATCY 4 cut(s) 60, 203, 691, 881
RsaI GTAC 6 cut(s) 387, 920, 988, 1213, 1351, 1372
RsaNI GTAC 6 cut(s) 386, 919, 987, 1212, 1350, 1371
RseI CAYNNNNRTG 9 cut(s) 302, 861, 897, 936, 1011, 1086, 1395, 1845, 1868
SacI GAGCTC 1 cut(s) 284
SapI GCTCTTC 2 cut(s) 232, 369
SaqAI TTAA 3 cut(s) 129, 449, 710
SatI GCNGC 6 cut(s) 33, 428, 781, 889, 1050, 1519
Sau3AI GATC 4 cut(s) 60, 203, 691, 881
Sau96I GGNCC 9 cut(s) 511, 549, 550, 928, 1132, 1141, 1567, 1700, 1967
SchI GAGTC 2 cut(s) 28, 341
ScrFI CCNGG 6 cut(s) 138, 504, 630, 803, 1260, 1575
SduI GDGCHC 8 cut(s) 284, 418, 553, 968, 1253, 1289, 1427, 1648
SfaNI GCATC 8 cut(s) 784, 1083, 1158, 1163, 1178, 1547, 1977, 1999
SfcI CTRYAG 3 cut(s) 108, 425, 1182
SfiI GGCCNNNNNGGCC 1 cut(s) 1140
SinI GGWCC 2 cut(s) 511, 1700
SmiMI CAYNNNNRTG 9 cut(s) 302, 861, 897, 936, 1011, 1086, 1395, 1845, 1868
Sse9I AATT 7 cut(s) 37, 130, 350, 375, 787, 953, 1111
SseBI AGGCCT 1 cut(s) 1638
SsiI CCGC 3 cut(s) 1127, 1246, 1916
SspMI CTAG 5 cut(s) 390, 486, 636, 681, 1599
SstI GAGCTC 1 cut(s) 284
StuI AGGCCT 1 cut(s) 1638
StyD4I CCNGG 6 cut(s) 136, 502, 628, 801, 1258, 1573
StyI CCWWGG 5 cut(s) 432, 958, 1269, 1306, 1333
TaaI ACNGT 6 cut(s) 768, 916, 935, 1010, 1457, 1925
TaiI ACGT 2 cut(s) 222, 988
TaqI TCGA 3 cut(s) 338, 1045, 1171
TaqII GACCGA 1 cut(s) 499
TasI AATT 7 cut(s) 37, 130, 350, 375, 787, 953, 1111
TatI WGTACW 3 cut(s) 918, 1211, 1349
TfiI GAWTC 4 cut(s) 174, 473, 1850, 1931
Tru1I TTAA 3 cut(s) 129, 449, 710
Tru9I TTAA 3 cut(s) 129, 449, 710
TscAI CASTG 6 cut(s) 237, 423, 466, 921, 1258, 1528
TseFI GTSAC 3 cut(s) 730, 768, 1919
TseI GCWGC 6 cut(s) 32, 427, 780, 888, 1049, 1518
Tsp45I GTSAC 3 cut(s) 730, 768, 1919
TspDTI ATGAA 8 cut(s) 356, 564, 609, 1047, 1670, 1757, 1784, 1799
TspGWI ACGGA 1 cut(s) 196
TspRI CASTG 6 cut(s) 237, 423, 466, 921, 1258, 1528
VneI GTGCAC 4 cut(s) 414, 964, 1249, 1423
VpaK11BI GGWCC 2 cut(s) 511, 1700
XapI RAATTY 2 cut(s) 37, 953
XceI RCATGY 3 cut(s) 160, 709, 801
XcmI CCANNNNNNNNNTGG 3 cut(s) 439, 560, 1381
XmnI GAANNNNTTC 1 cut(s) 595
XspI CTAG 5 cut(s) 390, 486, 636, 681, 1599
Zsp2I ATGCAT 1 cut(s) 1992
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.