Rorug07G0030100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0009121
Physical Location & Seq
Forward (+)
2083873 .. 2084547
675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0030100.1

Sequence Viewer

Length: 675 bp
ATGTTGACCTCATCAGGCTTACGCTTCCACCAGCTATCCTGCCTTACACTTTTGGTACTGCTTCTTCTTCCCCTCCCCTCCCATTCCGCAGACCCTGATCCATTACAAGACTTCTGTGTTGCAGATGTGAGTGCCTCCCTATCAGTTAATGGCTTTCCTTGCAAGGCCTCATCGCAGATCACCTCAGATGATTTTTTCTTTGACGGACTGAGCAAAGAAGGGAACACAACCAATCTGTACAGGGCGACTGTAACAGCTGGGAATGTCCTTGCATTTCCCGGGCTGAACACCCTTGGGATATCGATGAACAGAGTTGACTTTGCTCCTGGTGGACTCAATCCACCACACTCACACCCTCGGGCAAGTGAGACTGGGGTGGTCATTAAAGGAAAGCTGCTTGTGGGATTTGTGACAACTAACAATGTGTATTACTCCAAGGTCTTGACTGCTGGGCAGATGTTTGTGGTTCCGAGGGGACTTGTTCACTATCAGCTAAACGTTGGAAAAGGGAAGGCCCTTGCCTTCACAGCTTTCAACAGCCACTTACCAGGCTCTTCTGTGCTTTCTTTTAATCTGTTTGCTTCGACGCCTTCCATCCCTGATGCAGTGTTAACCAAGGCCTTTCAAGTAGATGATGATGTTATCAACAACATTCGATCCAAGTTTGGCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

224

Amino Acids

23.88

Weight (kDa)

7.77

Isoelectric Point (pI)

19.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 70 - 213 1.3e-44 Cupin
Cupin_2 PF07883 104 - 174 3.6e-10 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 87
AclI AACGTT 1 cut(s) 498
AclWI GGATC 2 cut(s) 92, 651
AcyI GRCGYC 1 cut(s) 587
AfaI GTAC 2 cut(s) 57, 239
AgsI TTSAA 2 cut(s) 535, 626
AjnI CCWGG 2 cut(s) 325, 547
AluBI AGCT 5 cut(s) 34, 257, 394, 493, 530
AluI AGCT 5 cut(s) 34, 257, 394, 493, 530
Alw26I GTCTC 1 cut(s) 362
AlwI GGATC 2 cut(s) 92, 651
AlwNI CAGNNNCTG 1 cut(s) 95
Ama87I CYCGRG 2 cut(s) 278, 357
AoxI GGCC 3 cut(s) 165, 513, 618
ApeKI GCWGC 1 cut(s) 394
AspS9I GGNCC 1 cut(s) 514
AsuC2I CCSGG 2 cut(s) 279, 280
AsuHPI GGTGA 1 cut(s) 172
AvaI CYCGRG 2 cut(s) 278, 357
BarI GAAGNNNNNNTAC 2 cut(s) 48, 80
BbvI GCAGC 1 cut(s) 381
BccI CCATC 1 cut(s) 602
BciT130I CCWGG 2 cut(s) 327, 549
BcnI CCSGG 2 cut(s) 279, 280
BcoDI GTCTC 1 cut(s) 362
BisI GCNGC 1 cut(s) 395
BlsI GCNGC 1 cut(s) 396
Bme1390I CCNGG 4 cut(s) 279, 280, 327, 549
BmeT110I CYCGRG 2 cut(s) 278, 357
BmgT120I GGNCC 1 cut(s) 514
BmiI GGNNCC 1 cut(s) 468
BmrFI CCNGG 4 cut(s) 279, 280, 327, 549
BmrI ACTGGG 1 cut(s) 381
BmsI GCATC 1 cut(s) 592
BmuI ACTGGG 1 cut(s) 381
BpuMI CCSGG 2 cut(s) 279, 280
Bsa29I ATCGAT 1 cut(s) 302
BsaHI GRCGYC 1 cut(s) 587
BsaJI CCNNGG 6 cut(s) 278, 292, 356, 435, 470, 615
Bse1I ACTGG 1 cut(s) 376
BseBI CCWGG 2 cut(s) 327, 549
BseCI ATCGAT 1 cut(s) 302
BseDI CCNNGG 6 cut(s) 278, 292, 356, 435, 470, 615
BseGI GGATG 1 cut(s) 594
BseMII CTCAG 2 cut(s) 198, 200
BseNI ACTGG 1 cut(s) 376
BseXI GCAGC 1 cut(s) 381
BseYI CCCAGC 2 cut(s) 257, 449
BshFI GGCC 3 cut(s) 167, 515, 620
BshVI ATCGAT 1 cut(s) 302
BsiHKCI CYCGRG 2 cut(s) 278, 357
BsiSI CCGG 1 cut(s) 279
BslFI GGGAC 1 cut(s) 489
BsmAI GTCTC 1 cut(s) 362
BsmFI GGGAC 1 cut(s) 489
BsnI GGCC 3 cut(s) 167, 515, 620
BsoBI CYCGRG 2 cut(s) 278, 357
Bsp1407I TGTACA 1 cut(s) 237
Bsp143I GATC 3 cut(s) 97, 177, 656
BspACI CCGC 1 cut(s) 87
BspANI GGCC 3 cut(s) 167, 515, 620
BspCNI CTCAG 2 cut(s) 197, 201
BspDI ATCGAT 1 cut(s) 302
BspLI GGNNCC 1 cut(s) 468
BspPI GGATC 2 cut(s) 92, 651
BspQI GCTCTTC 1 cut(s) 559
BsrGI TGTACA 1 cut(s) 237
BsrI ACTGG 1 cut(s) 376
BssECI CCNNGG 6 cut(s) 278, 292, 356, 435, 470, 615
BssMI GATC 3 cut(s) 97, 177, 656
BssNI GRCGYC 1 cut(s) 587
BssT1I CCWWGG 3 cut(s) 292, 435, 615
Bst2UI CCWGG 2 cut(s) 327, 549
Bst4CI ACNGT 1 cut(s) 250
Bst6I CTCTTC 1 cut(s) 559
BstACI GRCGYC 1 cut(s) 587
BstAUI TGTACA 1 cut(s) 237
BstDEI CTNAG 2 cut(s) 184, 209
BstF5I GGATG 1 cut(s) 594
BstKTI GATC 3 cut(s) 100, 180, 659
BstMAI GTCTC 1 cut(s) 362
BstMBI GATC 3 cut(s) 97, 177, 656
BstMWI GCNNNNNNNGC 2 cut(s) 159, 527
BstNI CCWGG 2 cut(s) 327, 549
BstSCI CCNGG 4 cut(s) 277, 278, 325, 547
BstV1I GCAGC 1 cut(s) 381
Bsu15I ATCGAT 1 cut(s) 302
BsuRI GGCC 3 cut(s) 167, 515, 620
BsuTUI ATCGAT 1 cut(s) 302
BtgZI GCGATG 1 cut(s) 156
BtsCI GGATG 1 cut(s) 594
BtsI GCAGTG 1 cut(s) 612
BtsIMutI CAGTG 1 cut(s) 612
CaiI CAGNNNCTG 1 cut(s) 95
Cfr13I GGNCC 1 cut(s) 514
Cfr9I CCCGGG 1 cut(s) 278
ClaI ATCGAT 1 cut(s) 302
CseI GACGC 1 cut(s) 595
Csp6I GTAC 2 cut(s) 56, 238
CviQI GTAC 2 cut(s) 56, 238
DdeI CTNAG 2 cut(s) 184, 209
DpnI GATC 3 cut(s) 99, 179, 658
DpnII GATC 3 cut(s) 97, 177, 656
Eam1104I CTCTTC 1 cut(s) 559
EarI CTCTTC 1 cut(s) 559
Eco130I CCWWGG 3 cut(s) 292, 435, 615
Eco147I AGGCCT 2 cut(s) 167, 620
Eco32I GATATC 1 cut(s) 300
Eco88I CYCGRG 2 cut(s) 278, 357
EcoO109I RGGNCCY 1 cut(s) 514
EcoRII CCWGG 2 cut(s) 325, 547
EcoRV GATATC 1 cut(s) 300
EcoT14I CCWWGG 3 cut(s) 292, 435, 615
ErhI CCWWGG 3 cut(s) 292, 435, 615
FalI AAGNNNNNCTT 1 cut(s) 653
FaqI GGGAC 1 cut(s) 489
Fnu4HI GCNGC 1 cut(s) 395
FokI GGATG 1 cut(s) 581
Fsp4HI GCNGC 1 cut(s) 395
GluI GCNGC 1 cut(s) 395
GsaI CCCAGC 2 cut(s) 261, 453
HaeIII GGCC 3 cut(s) 167, 515, 620
HapII CCGG 1 cut(s) 279
HgaI GACGC 1 cut(s) 595
Hin1I GRCGYC 1 cut(s) 587
HincII GTYRAC 3 cut(s) 6, 316, 612
HindII GTYRAC 3 cut(s) 6, 316, 612
HinfI GANTC 1 cut(s) 333
HpaI GTTAAC 1 cut(s) 612
HpaII CCGG 1 cut(s) 279
HphI GGTGA 1 cut(s) 172
Hpy166II GTNNAC 5 cut(s) 6, 316, 332, 484, 612
Hpy188I TCNGA 2 cut(s) 187, 471
Hpy188III TCNNGA 1 cut(s) 442
Hpy8I GTNNAC 5 cut(s) 6, 316, 332, 484, 612
Hpy99I CGWCG 1 cut(s) 589
HpyAV CCTTC 4 cut(s) 212, 505, 532, 600
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4IV ACGT 1 cut(s) 498
HpyCH4V TGCA 4 cut(s) 122, 162, 272, 605
HpyF10VI GCNNNNNNNGC 2 cut(s) 159, 527
HpyF3I CTNAG 2 cut(s) 184, 209
HpySE526I ACGT 1 cut(s) 498
Hsp92I GRCGYC 1 cut(s) 587
KspAI GTTAAC 1 cut(s) 612
Kzo9I GATC 3 cut(s) 97, 177, 656
LguI GCTCTTC 1 cut(s) 559
LmnI GCTCC 1 cut(s) 328
Lsp1109I GCAGC 1 cut(s) 381
LweI GCATC 1 cut(s) 592
MaeII ACGT 1 cut(s) 498
MaeIII GTNAC 2 cut(s) 250, 409
MalI GATC 3 cut(s) 99, 179, 658
MboI GATC 3 cut(s) 97, 177, 656
MboII GAAGA 3 cut(s) 56, 59, 546
MlyI GAGTC 1 cut(s) 327
MmeI TCCRAC 1 cut(s) 481
MnlI CCTC 8 cut(s) 19, 83, 88, 145, 178, 193, 366, 465
MseI TTAA 4 cut(s) 147, 384, 570, 611
MspA1I CMGCKG 1 cut(s) 257
MspI CCGG 1 cut(s) 279
MspR9I CCNGG 4 cut(s) 279, 280, 327, 549
MvaI CCWGG 2 cut(s) 327, 549
MwoI GCNNNNNNNGC 2 cut(s) 159, 527
NciI CCSGG 2 cut(s) 279, 280
NdeII GATC 3 cut(s) 97, 177, 656
NlaIV GGNNCC 1 cut(s) 468
NmuCI GTSAC 1 cut(s) 409
PceI AGGCCT 2 cut(s) 167, 620
PciSI GCTCTTC 1 cut(s) 559
PkrI GCNGC 1 cut(s) 396
PleI GAGTC 1 cut(s) 327
PpsI GAGTC 1 cut(s) 327
Psp1406I AACGTT 1 cut(s) 498
Psp6I CCWGG 2 cut(s) 325, 547
PspFI CCCAGC 2 cut(s) 257, 449
PspGI CCWGG 2 cut(s) 325, 547
PspN4I GGNNCC 1 cut(s) 468
PspPI GGNCC 1 cut(s) 514
PstNI CAGNNNCTG 1 cut(s) 95
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 2 cut(s) 57, 239
RsaNI GTAC 2 cut(s) 56, 238
SapI GCTCTTC 1 cut(s) 559
SaqAI TTAA 4 cut(s) 147, 384, 570, 611
SatI GCNGC 1 cut(s) 395
Sau3AI GATC 3 cut(s) 97, 177, 656
Sau96I GGNCC 1 cut(s) 514
SchI GAGTC 1 cut(s) 327
ScrFI CCNGG 4 cut(s) 279, 280, 327, 549
SetI ASST 9 cut(s) 11, 36, 185, 259, 396, 441, 495, 501, 532
SfaNI GCATC 1 cut(s) 592
SmaI CCCGGG 1 cut(s) 280
SseBI AGGCCT 2 cut(s) 167, 620
SsiI CCGC 1 cut(s) 87
StuI AGGCCT 2 cut(s) 167, 620
StyD4I CCNGG 4 cut(s) 277, 278, 325, 547
StyI CCWWGG 3 cut(s) 292, 435, 615
TaaI ACNGT 1 cut(s) 250
TaiI ACGT 1 cut(s) 501
TaqI TCGA 3 cut(s) 302, 584, 655
TatI WGTACW 1 cut(s) 237
Tru1I TTAA 4 cut(s) 147, 384, 570, 611
Tru9I TTAA 4 cut(s) 147, 384, 570, 611
TscAI CASTG 1 cut(s) 612
TseFI GTSAC 1 cut(s) 409
TseI GCWGC 1 cut(s) 394
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 1 cut(s) 320
TspGWI ACGGA 1 cut(s) 219
TspMI CCCGGG 1 cut(s) 278
TspRI CASTG 1 cut(s) 612
XmaI CCCGGG 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.