Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0010566

Physical Location & Seq

Genomic Coordinates Forward (+)

22964272 .. 22967667

Gene Structure 3396 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1139300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2358 bp

ATGTCTTTGATCCATACGTTGATGGATTCGGATACAAGAAGAAACCGGATGTGCAGACAAAACAACTACTCTCTTGTGGTTCTTGCTCTTTGTTTACTTCAACACTATCTCCATGTCTCTGTAACATTTGTTAATGCCACAAGCGAAGTGCACATTGTATATCTGGGGGAGAAGAAATATGATGATCCTGCGTTGACAAAAAAGTTCCATCATAAAATGCTAACCACCTTGTTTGGAAGCAAACAAGATGCATATAGATCGATCATATACAGCTACAAGTATGGATTTTCCGGCTTTGCTGCAAGATTAACGGAATCTCAGGCAGAAGAAATAGCAGAGTTTCCTGAAGTTGTCCAAGTGATCCCGAATCGCGTTCACAAGCTCCACACAACCCGAAGTTGGGATTTCATTGGGATTCATAAGTATTCGTCGGACAATCTTTTAACAAAGAGCATGGGCAAAGGAACTATCATTGGTGTAATAGATTCAGGTGTTTGGCCTGAATCCGAAAGCTTTAATGATGACGCCATGGGTCCTATCCCATCACATTGGAAAGGCATTTGCCAGCAGGGGGAATACTTCAACTCCACAAATTGCAACAAAAAAATTATTGGTGCACGCTGGTTTAGAAAAGGTGCGATGAATCAGTTCCAAAATCTCAACAAAACCGACAATGTGGACTTTCTGTCACCGCGGGATGGAATTGGACATGGCACTCATACAGCTTCTACAGCAGCTGGGTATTTTGTGAAGAATGCCAACTACAGAGGACTTGCTTCCGGGCTAGCCAGAGGAGGAGCCCCTCTTGCTCACTTGGCAATTTACAAGGCCTGCTGGGCCTTCGAAGGATGCACCGATGCTGATCTTCTAAAGGCATTTGACAAGGCCATTCATGATGGAGTAGACATCATCTCTCTTTCTGTGGGCAATGAGATTCCTTTGTTCTCATACGTTGATCAGCGTGATTCAATCGCAATTGGTTCGTTTCATGCAATGACGAAGGGAATTACAGTAGTTTGCTCAGCAGGAAATGATGGCCCTATCTCACAAACCATTGTAAACACTGCCCCATGGCTCATCACTGTTGCAGCCACCACAATCGACAGGGCCTTTCCGACAGCCATTACACTCGGAAACAATCAAACTCTTTGGGGCCAATCTATTGATGCTGGAAAATACAACCGTGAATTCGCTAGCATCACATACTCTGAACGCATAGCTATAGACCCAACAGATGATTCAGCCAAGGATTGTCAGCCTGGAAGTCTCAATGCAACATTAGCCTCAGGGAAAATAGTACTTTGTTTCTCAAAATCAGATCAACAGGACATCGAGTCTGCTGCAACCACCGTCAAGGACGCGGGAGGGGTTGGACTTATATTCGCACAGTTTCGTGATGACGGGCTCAGTTCATGCGACATTCCATGCATCAGTGTAGATTATGAAGTAGGGACACAAGTACTTTCCTACATCAGAAGAGCAAGGCATTCGATTGCAAAGCTCAGTGATCCAAAAACTACCATTGGGAAATGGGTCTCTCCACGAGTTGCGTCTTTCTCCGCTAGAGGACCTAGTTCCATGACACCAGAAGTGCTTAAGCCGGACATAGCTGCACCGGGAGTAGACATCATAGCTGCATTTCGCCCACTTGACACTGAACATCGCAGCGGTTATGCTCTGCTATCGGGAACTTCAATGGCATGCCCTCATGTCGCAGGAATAGCAGCTCTCATTAAATCTGCACATCCAAATTGGTCTCCTGCTGCAATAAAATCAGCTCTAGTCACTACTGCTTCCCAAACTGGAACAGATGGGACAAGTATTTCGGCTGAAGGCTTGATGCGCAAGGTAGCTGACCCTTTCGACATGGGAGGAGGACATGTTGATCCCAACAAGGCTATCGATCCGGGGCTCATCTTCGATAGTAGTACAAAGGATTACATTCAGTTCCTCTGCTCCCTCGGTGACACTAGCGCATCGATTACTAGATTAACCAAGAACACAATAAATTGCTCAACGAAAAGTCATGGAATGAACCTCAACCTCCCTTCTATCACAATTCCAAACCTCGAAAGGGCGACAACTGTGACAAGAACCGTGACAAATGTGGGACAAATTAACTCGAAGTATACGGTATTGGTGCAGGCTCCTTCTGGCGTAAAAATGACAGTTGAGCCTCAAAGTTTAAGTTTCAATATAACCAGCCAAATTCTTTCCTTCAAAGTTACCTTCTTCTCTGCCCAGAGAGTGAATGGAGGTTACAAGTTTGGGAGCCTAACTTGGACAGATGGTGAGCATATTGTCAGAAGTCCAGTAGCGATTCGCGTGATCGGATTCGAATCGTATGTTTATGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 Peptidase_S8

Protein Analysis

786

Amino Acids

85.11

Weight (kDa)

8.08

Isoelectric Point (pI)

29.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	50 - 129	1.4e-18	Peptidase inhibitor I9
Peptidase_S8	PF00082	153 - 603	2.3e-46	Subtilase family
fn3_6	PF17766	679 - 774	5.9e-31	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010566)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g10480 FvH4_5g10480 FvH4_5g10480 FvH4_5g10480 FvH4_5g10480 FvH4_5g10480 FvH4_5g10480
malus_domestica	MD14G1139300.v1.1
prunus_persica	Prupe.5G133800_v2.0.a1 Prupe.5G133800_v2.0.a1
pyrus_communis	pycom14g11500
rosa_chinensis	RchiOBHm_Chr7g0187701
rosa_laevigata	RLG00000004760
rosa_multiflora	Rmu_sc0004483.1_g000024
rosa_roxburghii	Rroxscaffold_3G00267150
rosa_rugosa	Rorug06G0483600 Rorug06G0483700
rosa_samantha	Rh7AG088800 Rh7BG090500 Rh7CG089500 Rh7DG091000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1844
AccI	GTMKAC	3 cut(s)	903, 1623, 2129
AccII	CGCG	4 cut(s)	372, 694, 1361, 2325
AciI	CCGC	5 cut(s)	692, 694, 1361, 1560, 1668
AclWI	GGATC	6 cut(s)	4, 179, 355, 1502, 1880, 1898
AcsI	RAATTY	2 cut(s)	1187, 2208
AcuI	CTGAAG	2 cut(s)	366, 1851
AcyI	GRCGYC	1 cut(s)	525
AfaI	GTAC	3 cut(s)	1299, 1461, 1930
AfiI	CCNNNNNNNGG	5 cut(s)	399, 400, 571, 698, 2074
AflII	CTTAAG	1 cut(s)	1595
AflIII	ACRYGT	1 cut(s)	1879
AgsI	TTSAA	6 cut(s)	101, 583, 969, 1695, 2194, 2221
AhdI	GACNNNNNGTC	2 cut(s)	685, 1333
AjnI	CCWGG	1 cut(s)	1258
AjuI	GAANNNNNNNTTGG	2 cut(s)	1222, 1254
Alw21I	GWGCWC	2 cut(s)	153, 619
Alw26I	GTCTC	4 cut(s)	121, 1271, 1540, 1761
Alw44I	GTGCAC	2 cut(s)	149, 615
AlwI	GGATC	6 cut(s)	4, 179, 355, 1502, 1880, 1898
AlwNI	CAGNNNCTG	1 cut(s)	737
AoxI	GGCC	7 cut(s)	497, 828, 837, 885, 1036, 1107, 1153
ApaLI	GTGCAC	2 cut(s)	149, 615
ApeKI	GCWGC	9 cut(s)	299, 734, 1088, 1340, 1610, 1634, 1665, 1724, 1763
ApoI	RAATTY	2 cut(s)	1187, 2208
ArsI	GACNNNNNNTTYG	2 cut(s)	1807, 1839
Asp700I	GAANNNNTTC	1 cut(s)	647
AspLEI	GCGC	2 cut(s)	1845, 1976
AspS9I	GGNCC	6 cut(s)	533, 837, 1037, 1107, 1153, 1568
AsuC2I	CCSGG	3 cut(s)	781, 1617, 1908
AsuHPI	GGTGA	3 cut(s)	681, 1976, 2303
AsuII	TTCGAA	2 cut(s)	843, 2337
AsuNHI	GCTAGC	2 cut(s)	784, 1193
AvaII	GGWCC	2 cut(s)	533, 1568
AxyI	CCTNAGG	1 cut(s)	1285
BaeGI	GKGCMC	2 cut(s)	153, 619
BanII	GRGCYC	3 cut(s)	802, 1407, 1914
BauI	CACGAG	1 cut(s)	1542
Bbv12I	GWGCWC	2 cut(s)	153, 619
BbvI	GCAGC	9 cut(s)	286, 746, 1100, 1327, 1597, 1621, 1677, 1736, 1750
BccI	CCATC	8 cut(s)	16, 216, 550, 692, 890, 1028, 1805, 2282
BcgI	CGANNNNNNTGC	8 cut(s)	240, 274, 963, 997, 1322, 1356, 1470, 1504
BciT130I	CCWGG	1 cut(s)	1260
BciVI	GTATCC	1 cut(s)	25
BclI	TGATCA	1 cut(s)	955
BcnI	CCSGG	3 cut(s)	781, 1617, 1908
BcoDI	GTCTC	4 cut(s)	121, 1271, 1540, 1761
BfaI	CTAG	7 cut(s)	785, 1194, 1563, 1572, 1781, 1971, 1986
BfmI	CTRYAG	3 cut(s)	729, 763, 1221
BfrI	CTTAAG	1 cut(s)	1595
BfuI	GTATCC	1 cut(s)	25
BglI	GCCNNNNNGGC	1 cut(s)	836
BisI	GCNGC	9 cut(s)	300, 735, 1089, 1341, 1611, 1635, 1666, 1725, 1764
BlpI	GCTNAGC	1 cut(s)	1021
BlsI	GCNGC	9 cut(s)	301, 736, 1090, 1342, 1612, 1636, 1667, 1726, 1765
BmcAI	AGTACT	2 cut(s)	1299, 1461
Bme1390I	CCNGG	4 cut(s)	781, 1260, 1617, 1908
Bme18I	GGWCC	2 cut(s)	533, 1568
BmeRI	GACNNNNNGTC	2 cut(s)	685, 1333
BmgT120I	GGNCC	6 cut(s)	533, 837, 1037, 1107, 1153, 1568
BmiI	GGNNCC	5 cut(s)	534, 799, 1154, 2148, 2273
BmrFI	CCNGG	4 cut(s)	781, 1260, 1617, 1908
BmsI	GCATC	8 cut(s)	238, 839, 847, 1156, 1206, 1437, 1830, 1985
BmtI	GCTAGC	2 cut(s)	788, 1197
Bpu1102I	GCTNAGC	1 cut(s)	1021
Bpu14I	TTCGAA	2 cut(s)	843, 2337
BpuMI	CCSGG	3 cut(s)	781, 1617, 1908
Bsa29I	ATCGAT	3 cut(s)	260, 1902, 1979
BsaBI	GATNNNNATC	2 cut(s)	861, 2338
BsaHI	GRCGYC	1 cut(s)	525
BsaI	GGTCTC	2 cut(s)	1540, 1761
BsaJI	CCNNGG	6 cut(s)	528, 692, 1070, 1245, 1907, 1960
BsaWI	WCCGGW	1 cut(s)	45
BsaXI	ACNNNNNCTCC	6 cut(s)	93, 123, 569, 599, 1866, 1896
Bsc4I	CCNNNNNNNGG	5 cut(s)	399, 400, 571, 698, 2074
Bse1I	ACTGG	2 cut(s)	1807, 2312
Bse21I	CCTNAGG	1 cut(s)	1285
Bse3DI	GCAATG	2 cut(s)	934, 999
Bse8I	GATNNNNATC	2 cut(s)	861, 2338
BseBI	CCWGG	1 cut(s)	1260
BseCI	ATCGAT	3 cut(s)	260, 1902, 1979
BseDI	CCNNGG	6 cut(s)	528, 692, 1070, 1245, 1907, 1960
BseGI	GGATG	4 cut(s)	54, 703, 854, 1744
BseJI	GATNNNNATC	2 cut(s)	861, 2338
BseLI	CCNNNNNNNGG	5 cut(s)	399, 400, 571, 698, 2074
BseMI	GCAATG	2 cut(s)	934, 999
BseMII	CTCAG	5 cut(s)	332, 1035, 1299, 1420, 1516
BseNI	ACTGG	2 cut(s)	1807, 2312
BseRI	GAGGAG	3 cut(s)	807, 810, 1887
BseSI	GKGCMC	2 cut(s)	153, 619
BseXI	GCAGC	9 cut(s)	286, 746, 1100, 1327, 1597, 1621, 1677, 1736, 1750
BseYI	CCCAGC	2 cut(s)	737, 834
BsgI	GTGCAG	4 cut(s)	73, 1596, 1725, 2162
Bsh1236I	CGCG	4 cut(s)	372, 694, 1361, 2325
BshFI	GGCC	7 cut(s)	499, 830, 839, 887, 1038, 1109, 1155
BshVI	ATCGAT	3 cut(s)	260, 1902, 1979
BsiHKAI	GWGCWC	2 cut(s)	153, 619
BsiSI	CCGG	6 cut(s)	46, 291, 780, 1601, 1616, 1907
BslFI	GGGAC	3 cut(s)	1465, 1828, 2124
BslI	CCNNNNNNNGG	5 cut(s)	399, 400, 571, 698, 2074
BsmAI	GTCTC	4 cut(s)	121, 1271, 1540, 1761
BsmFI	GGGAC	3 cut(s)	1465, 1828, 2124
BsmI	GAATGC	2 cut(s)	760, 1486
BsnI	GGCC	7 cut(s)	499, 830, 839, 887, 1038, 1109, 1155
Bso31I	GGTCTC	2 cut(s)	1540, 1761
Bsp119I	TTCGAA	2 cut(s)	843, 2337
Bsp1286I	GDGCHC	5 cut(s)	153, 619, 802, 1407, 1914
Bsp1720I	GCTNAGC	1 cut(s)	1021
Bsp19I	CCATGG	2 cut(s)	528, 1070
BspACI	CCGC	5 cut(s)	692, 694, 1361, 1560, 1668
BspANI	GGCC	7 cut(s)	499, 830, 839, 887, 1038, 1109, 1155
BspCNI	CTCAG	5 cut(s)	331, 1034, 1298, 1419, 1515
BspDI	ATCGAT	3 cut(s)	260, 1902, 1979
BspFNI	CGCG	4 cut(s)	372, 694, 1361, 2325
BspHI	TCATGA	1 cut(s)	892
BspLI	GGNNCC	5 cut(s)	534, 799, 1154, 2148, 2273
BspOI	GCTAGC	2 cut(s)	788, 1197
BspPI	GGATC	6 cut(s)	4, 179, 355, 1502, 1880, 1898
BspQI	GCTCTTC	1 cut(s)	1471
BspT104I	TTCGAA	2 cut(s)	843, 2337
BspTI	CTTAAG	1 cut(s)	1595
BspTNI	GGTCTC	2 cut(s)	1540, 1761
BsrDI	GCAATG	2 cut(s)	934, 999
BsrI	ACTGG	2 cut(s)	1807, 2312
BssECI	CCNNGG	6 cut(s)	528, 692, 1070, 1245, 1907, 1960
BssNAI	GTATAC	1 cut(s)	2130
BssNI	GRCGYC	1 cut(s)	525
BssSI	CACGAG	1 cut(s)	1542
BssT1I	CCWWGG	3 cut(s)	528, 1070, 1245
Bst1107I	GTATAC	1 cut(s)	2130
Bst2BI	CACGAG	1 cut(s)	1542
Bst2UI	CCWGG	1 cut(s)	1260
Bst4CI	ACNGT	9 cut(s)	1012, 1084, 1184, 1351, 1389, 2086, 2098, 2134, 2170
Bst6I	CTCTTC	1 cut(s)	1471
BstACI	GRCGYC	1 cut(s)	525
BstAFI	CTTAAG	1 cut(s)	1595
BstBI	TTCGAA	2 cut(s)	843, 2337
BstC8I	GCNNGC	7 cut(s)	566, 619, 786, 832, 1195, 1702, 2145
BstDEI	CTNAG	5 cut(s)	318, 1021, 1285, 1406, 1502
BstDSI	CCRYGG	3 cut(s)	528, 692, 1070
BstF5I	GGATG	4 cut(s)	54, 703, 854, 1744
BstFNI	CGCG	4 cut(s)	372, 694, 1361, 2325
BstHHI	GCGC	2 cut(s)	1845, 1976
BstMAI	GTCTC	4 cut(s)	121, 1271, 1540, 1761
BstMWI	GCNNNNNNNGC	7 cut(s)	731, 806, 815, 836, 1280, 1721, 1842
BstNI	CCWGG	1 cut(s)	1260
BstNSI	RCATGY	2 cut(s)	1704, 1883
BstSCI	CCNGG	4 cut(s)	779, 1258, 1615, 1906
BstSFI	CTRYAG	3 cut(s)	729, 763, 1221
BstSLI	GKGCMC	2 cut(s)	153, 619
BstUI	CGCG	4 cut(s)	372, 694, 1361, 2325
BstV1I	GCAGC	9 cut(s)	286, 746, 1100, 1327, 1597, 1621, 1677, 1736, 1750
BstXI	CCANNNNNNTGG	1 cut(s)	549
BstZ17I	GTATAC	1 cut(s)	2130
Bsu15I	ATCGAT	3 cut(s)	260, 1902, 1979
Bsu36I	CCTNAGG	1 cut(s)	1285
BsuI	GTATCC	1 cut(s)	25
BsuRI	GGCC	7 cut(s)	499, 830, 839, 887, 1038, 1109, 1155
BsuTUI	ATCGAT	3 cut(s)	260, 1902, 1979
BtgI	CCRYGG	3 cut(s)	528, 692, 1070
BtgZI	GCGATG	2 cut(s)	653, 1646
BtsCI	GGATG	4 cut(s)	54, 703, 854, 1744
BtsI	GCAGTG	1 cut(s)	1062
BtsIMutI	CAGTG	5 cut(s)	1062, 1080, 1438, 1510, 1653
Cac8I	GCNNGC	7 cut(s)	566, 619, 786, 832, 1195, 1702, 2145
CaiI	CAGNNNCTG	1 cut(s)	737
CciI	TCATGA	1 cut(s)	892
CfoI	GCGC	2 cut(s)	1845, 1976
Cfr13I	GGNCC	6 cut(s)	533, 837, 1037, 1107, 1153, 1568
Cfr42I	CCGCGG	1 cut(s)	695
ClaI	ATCGAT	3 cut(s)	260, 1902, 1979
CseI	GACGC	3 cut(s)	533, 1367, 1539
Csp6I	GTAC	3 cut(s)	1298, 1460, 1929
CspCI	CAANNNNNGTGG	2 cut(s)	214, 249
CviQI	GTAC	3 cut(s)	1298, 1460, 1929
DdeI	CTNAG	5 cut(s)	318, 1021, 1285, 1406, 1502
DriI	GACNNNNNGTC	2 cut(s)	685, 1333
Eam1104I	CTCTTC	1 cut(s)	1471
Eam1105I	GACNNNNNGTC	2 cut(s)	685, 1333
EarI	CTCTTC	1 cut(s)	1471
Eco130I	CCWWGG	3 cut(s)	528, 1070, 1245
Eco147I	AGGCCT	1 cut(s)	830
Eco24I	GRGCYC	3 cut(s)	802, 1407, 1914
Eco31I	GGTCTC	2 cut(s)	1540, 1761
Eco47I	GGWCC	2 cut(s)	533, 1568
Eco57I	CTGAAG	2 cut(s)	366, 1851
Eco81I	CCTNAGG	1 cut(s)	1285
EcoO109I	RGGNCCY	3 cut(s)	533, 1107, 1568
EcoRI	GAATTC	1 cut(s)	1187
EcoRII	CCWGG	1 cut(s)	1258
EcoT14I	CCWWGG	3 cut(s)	528, 1070, 1245
EcoT22I	ATGCAT	2 cut(s)	253, 1430
EcoT38I	GRGCYC	3 cut(s)	802, 1407, 1914
ErhI	CCWWGG	3 cut(s)	528, 1070, 1245
FaqI	GGGAC	3 cut(s)	1465, 1828, 2124
FauI	CCCGC	2 cut(s)	687, 1354
FbaI	TGATCA	1 cut(s)	955
FblI	GTMKAC	3 cut(s)	903, 1623, 2129
Fnu4HI	GCNGC	9 cut(s)	300, 735, 1089, 1341, 1611, 1635, 1666, 1725, 1764
FokI	GGATG	4 cut(s)	61, 710, 861, 1731
FriOI	GRGCYC	3 cut(s)	802, 1407, 1914
Fsp4HI	GCNGC	9 cut(s)	300, 735, 1089, 1341, 1611, 1635, 1666, 1725, 1764
FspBI	CTAG	7 cut(s)	785, 1194, 1563, 1572, 1781, 1971, 1986
FspI	TGCGCA	1 cut(s)	1844
GlaI	GCGC	2 cut(s)	1844, 1975
GluI	GCNGC	9 cut(s)	300, 735, 1089, 1341, 1611, 1635, 1666, 1725, 1764
GsaI	CCCAGC	2 cut(s)	741, 838
HaeIII	GGCC	7 cut(s)	499, 830, 839, 887, 1038, 1109, 1155
HapII	CCGG	6 cut(s)	46, 291, 780, 1601, 1616, 1907
HgaI	GACGC	3 cut(s)	533, 1367, 1539
HhaI	GCGC	2 cut(s)	1845, 1976
Hin1I	GRCGYC	1 cut(s)	525
Hin6I	GCGC	2 cut(s)	1843, 1974
HinP1I	GCGC	2 cut(s)	1843, 1974
HincII	GTYRAC	1 cut(s)	195
HindII	GTYRAC	1 cut(s)	195
HindIII	AAGCTT	1 cut(s)	511
HpaII	CCGG	6 cut(s)	46, 291, 780, 1601, 1616, 1907
HphI	GGTGA	3 cut(s)	681, 1976, 2303
Hpy188III	TCNNGA	5 cut(s)	344, 364, 893, 1394, 1686
Hpy99I	CGWCG	1 cut(s)	433
HpyAV	CCTTC	8 cut(s)	839, 850, 994, 1826, 2058, 2160, 2227, 2239
HpyCH4III	ACNGT	9 cut(s)	1012, 1084, 1184, 1351, 1389, 2086, 2098, 2134, 2170
HpyCH4IV	ACGT	2 cut(s)	17, 951
HpyF10VI	GCNNNNNNNGC	7 cut(s)	731, 806, 815, 836, 1280, 1721, 1842
HpyF3I	CTNAG	5 cut(s)	318, 1021, 1285, 1406, 1502
HpySE526I	ACGT	2 cut(s)	17, 951
Hsp92I	GRCGYC	1 cut(s)	525
HspAI	GCGC	2 cut(s)	1843, 1974
Ksp22I	TGATCA	1 cut(s)	955
KspI	CCGCGG	1 cut(s)	695
LguI	GCTCTTC	1 cut(s)	1471
LmnI	GCTCC	5 cut(s)	387, 797, 1961, 2152, 2271
Lsp1109I	GCAGC	9 cut(s)	286, 746, 1100, 1327, 1597, 1621, 1677, 1736, 1750
LweI	GCATC	8 cut(s)	238, 839, 847, 1156, 1206, 1437, 1830, 1985
MaeI	CTAG	7 cut(s)	785, 1194, 1563, 1572, 1781, 1971, 1986
MaeII	ACGT	2 cut(s)	17, 951
MaeIII	GTNAC	8 cut(s)	121, 687, 1783, 1964, 2086, 2098, 2224, 2258
MboII	GAAGA	8 cut(s)	51, 184, 338, 763, 857, 1488, 1909, 2224
MfeI	CAATTG	1 cut(s)	975
MhlI	GDGCHC	5 cut(s)	153, 619, 802, 1407, 1914
MlyI	GAGTC	1 cut(s)	1343
MmeI	TCCRAC	3 cut(s)	411, 1139, 1351
Mph1103I	ATGCAT	2 cut(s)	253, 1430
MroXI	GAANNNNTTC	1 cut(s)	647
MseI	TTAA	9 cut(s)	132, 308, 443, 516, 1596, 1734, 1991, 2118, 2186
MspA1I	CMGCKG	3 cut(s)	694, 737, 1668
MspCI	CTTAAG	1 cut(s)	1595
MspI	CCGG	6 cut(s)	46, 291, 780, 1601, 1616, 1907
MspR9I	CCNGG	4 cut(s)	781, 1260, 1617, 1908
MunI	CAATTG	1 cut(s)	975
Mva1269I	GAATGC	2 cut(s)	760, 1486
MvaI	CCWGG	1 cut(s)	1260
MvnI	CGCG	4 cut(s)	372, 694, 1361, 2325
MwoI	GCNNNNNNNGC	7 cut(s)	731, 806, 815, 836, 1280, 1721, 1842
NciI	CCSGG	3 cut(s)	781, 1617, 1908
NcoI	CCATGG	2 cut(s)	528, 1070
NheI	GCTAGC	2 cut(s)	784, 1193
NlaIV	GGNNCC	5 cut(s)	534, 799, 1154, 2148, 2273
NmuCI	GTSAC	5 cut(s)	687, 1783, 1964, 2086, 2098
NsbI	TGCGCA	1 cut(s)	1844
NsiI	ATGCAT	2 cut(s)	253, 1430
NspI	RCATGY	2 cut(s)	1704, 1883
NspV	TTCGAA	2 cut(s)	843, 2337
PaeI	GCATGC	1 cut(s)	1704
PagI	TCATGA	1 cut(s)	892
PceI	AGGCCT	1 cut(s)	830
PciI	ACATGT	1 cut(s)	1879
PciSI	GCTCTTC	1 cut(s)	1471
PctI	GAATGC	2 cut(s)	760, 1486
PdmI	GAANNNNTTC	1 cut(s)	647
PkrI	GCNGC	9 cut(s)	301, 736, 1090, 1342, 1612, 1636, 1667, 1726, 1765
PleI	GAGTC	1 cut(s)	1342
PpsI	GAGTC	1 cut(s)	1342
PpuMI	RGGWCCY	2 cut(s)	533, 1568
PscI	ACATGT	1 cut(s)	1879
Psp5II	RGGWCCY	2 cut(s)	533, 1568
Psp6I	CCWGG	1 cut(s)	1258
PspFI	CCCAGC	2 cut(s)	737, 834
PspGI	CCWGG	1 cut(s)	1258
PspN4I	GGNNCC	5 cut(s)	534, 799, 1154, 2148, 2273
PspPI	GGNCC	6 cut(s)	533, 837, 1037, 1107, 1153, 1568
PspPPI	RGGWCCY	2 cut(s)	533, 1568
PstNI	CAGNNNCTG	1 cut(s)	737
PvuII	CAGCTG	1 cut(s)	737
RsaI	GTAC	3 cut(s)	1299, 1461, 1930
RsaNI	GTAC	3 cut(s)	1298, 1460, 1929
SacII	CCGCGG	1 cut(s)	695
SapI	GCTCTTC	1 cut(s)	1471
SaqAI	TTAA	9 cut(s)	132, 308, 443, 516, 1596, 1734, 1991, 2118, 2186
SatI	GCNGC	9 cut(s)	300, 735, 1089, 1341, 1611, 1635, 1666, 1725, 1764
Sau96I	GGNCC	6 cut(s)	533, 837, 1037, 1107, 1153, 1568
ScaI	AGTACT	2 cut(s)	1299, 1461
SchI	GAGTC	1 cut(s)	1343
ScrFI	CCNGG	4 cut(s)	781, 1260, 1617, 1908
SduI	GDGCHC	5 cut(s)	153, 619, 802, 1407, 1914
SfaNI	GCATC	8 cut(s)	238, 839, 847, 1156, 1206, 1437, 1830, 1985
SfcI	CTRYAG	3 cut(s)	729, 763, 1221
SfiI	GGCCNNNNNGGCC	1 cut(s)	836
Sfr303I	CCGCGG	1 cut(s)	695
SfuI	TTCGAA	2 cut(s)	843, 2337
SgrBI	CCGCGG	1 cut(s)	695
SinI	GGWCC	2 cut(s)	533, 1568
SmlI	CTYRAG	1 cut(s)	1595
SmoI	CTYRAG	1 cut(s)	1595
SphI	GCATGC	1 cut(s)	1704
SseBI	AGGCCT	1 cut(s)	830
SsiI	CCGC	5 cut(s)	692, 694, 1361, 1560, 1668
SspMI	CTAG	7 cut(s)	785, 1194, 1563, 1572, 1781, 1971, 1986
StuI	AGGCCT	1 cut(s)	830
StyD4I	CCNGG	4 cut(s)	779, 1258, 1615, 1906
StyI	CCWWGG	3 cut(s)	528, 1070, 1245
TaaI	ACNGT	9 cut(s)	1012, 1084, 1184, 1351, 1389, 2086, 2098, 2134, 2170
TaiI	ACGT	2 cut(s)	20, 954
TatI	WGTACW	3 cut(s)	1297, 1459, 1928
Tru1I	TTAA	9 cut(s)	132, 308, 443, 516, 1596, 1734, 1991, 2118, 2186
Tru9I	TTAA	9 cut(s)	132, 308, 443, 516, 1596, 1734, 1991, 2118, 2186
TscAI	CASTG	5 cut(s)	1069, 1087, 1438, 1510, 1660
TseFI	GTSAC	5 cut(s)	687, 1783, 1964, 2086, 2098
TseI	GCWGC	9 cut(s)	299, 734, 1088, 1340, 1610, 1634, 1665, 1724, 1763
Tsp45I	GTSAC	5 cut(s)	687, 1783, 1964, 2086, 2098
TspDTI	ATGAA	8 cut(s)	397, 407, 656, 881, 977, 1401, 1458, 2048
TspGWI	ACGGA	1 cut(s)	326
TspRI	CASTG	5 cut(s)	1069, 1087, 1438, 1510, 1660
Vha464I	CTTAAG	1 cut(s)	1595
VneI	GTGCAC	2 cut(s)	149, 615
VpaK11BI	GGWCC	2 cut(s)	533, 1568
XapI	RAATTY	2 cut(s)	1187, 2208
XceI	RCATGY	2 cut(s)	1704, 1883
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2249
XmiI	GTMKAC	3 cut(s)	903, 1623, 2129
XmnI	GAANNNNTTC	1 cut(s)	647
XspI	CTAG	7 cut(s)	785, 1194, 1563, 1572, 1781, 1971, 1986
ZrmI	AGTACT	2 cut(s)	1299, 1461
Zsp2I	ATGCAT	2 cut(s)	253, 1430

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.