Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0010566

Physical Location & Seq

Genomic Coordinates Reverse (-)

61995130 .. 61999295

Gene Structure 4166 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000004760

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2421 bp

ATGGATGGAGGATTCGGCTTGCATTCAGGGACATCAATGGCAACTCCCCATATTGCAGGCATTGTAGCACTTTTAAAAGCATTGCACCCAGATTGGTCTCCTGCTGCAATCAAAGGCATTGTGGGAAAGAACTACTCTCTGGTGGTTCTTGCTTTCATCTTATTACTTCAACATCATCTGTATACATTAGTCGAGGCCACAAGTAAAGTGCACATTGTATATTTGGGAGAAAAGAAACATGATGATCCAGCATCTACAAAGAAGTTTCATCATAAAATTCTGACCAACTTGTTAGGAAGCAAAGAAGCTGCATACAGATCCATTCTCTATAGCTATAAGCATGGCTTTTCTGGATTTGCTGCAAGATTAACAGAATCTCAAGCAGAAACAATTGCTGAGTTTCCAGAAGTTGTCCAAGTGATTCCCAATCGCATTCACAAGCTCCATACAACCAGAAGTTGGGATTTCATCGGAATCCATCAGCATTCTCTAGAAAATCATTTAACAAGAAGCATGGGCAAAGGAACTATAATTGGAGTAATAGATTCAGGTGTTTGGCCAGAGTCTGAAAGTTTCAAGGACGAAGGCATGGATCCTATCCCATCACATTGGAAAGGAACTTGCCAGCAGGGTGAGCTTTTTAACTCCACAAATTGCAATAAGAAACTTATTGGTGCTCGCTGGTTTGTAAAAGGAGCGCTGGATGAATCCAAAACACCAATTAACAAAACTGAGGGAGAAGACTTTCTGTCACCGAGGGATGGAATTGGCCATGGCACTCATACAGCTTCTACAGCGGCTGGCTATTTTGTCAAAAGAGCCAACTATAGAGGACTAGCTTCAGGATTAGCCAGAGGAGGAGCGCCTCTTGCTCACTTAGCAATTTACAAGGCTTGCTGGAGCAATAGAGGATGCACTGATGCTGATATTCTTAAAGCATTTGACAAGGCCATTCATGATGGAGTGGACATCCTATCTGTTTCTGTAGGCAATGAGATTCCTTTGTTCTCTTATGTCGATCTACGTGATACAATTGCAATTGGTTCCTTCCATGCAACCATGAAGGGGATTACAGTTGTTTGCTCAGCTGGAAATGATGGCCCTATCTCGCAGACTATTGTAAACACTGCACCATGGCTCATCACTGTTGCAGCCACCAAAATAGACAGGGCATTTCCAGCAGCCATTACACTTGGAAATAACCAGACTCTTTGGGGTCAATCTATTGATATTGAAAAGCATCACAATGGATTTGCAGGCCTCACATACTCTGAACGCATAGCTATAGACCCTATGGATGATTCAGCCAAGGATTGTCAGCCTGGAAGTCTCAATGCAACATTAGCCTCAGGGAAAATTGTACTCTGTTTCTCCATATCAGATGAACAAGACATAGTTTCTGCATCAGCTACCGTGAAAAAGGCTGGTGGAATTGGACTAATATATGCAGAGTTTCCCAATGATGGGCTCGAGCCATGCAAAATTCCATGCATTAAAGTAGATTATGAAGTAGGAACACAAATACTTTTCTACATCAGAAAAGCAAGGTACCCAATTGCCAAGCTCAGTGATCCAACCACCGTTGTTGGAAAATGGGTCTCTCCACAAGTTGCTACTTTCTCATCTAGGGGACCTAGTTCCATGACACCAGCAGTTTTAAAGCCTGACATAGCGGCACCTGGCGTGGACATCATAGCTGCATTTCGACCGCATGAGGAAAAACAAAGCAATGGTTATGCACTGCTATCAGGAACTTCAATGGCATGTCCTCATGTGACAGGGATTGTAGCTCTCATCAAATCTGTACATCAAGATTGGTCTCCTGCAGCAATAAAATCAGCTCTAGTCACTACTGCGTCCCAGACTGGAACAGATGGAACAAGCATTTCTGCCGAAGGCCCAACTCGCAAGGAAGCTGACCCATTTGACATAGGTGGTGGGCATGTTGATCCCAACAAGGCAATGGATCCCGGGCTAATTTACAATGCAACTGCAGACGACTACATTCAGTTTCTCTGCTCCCTGGGTTACAGTAGTGCATCACTTACTAGATTAACCAAGACTACATTAAACTGCAGAAGAAAAACTGATGCAATGACTCTCAACCTCCCTTCTATCGCAATTCCAAACCTGAAAAAGAGCGCAACGGTGACAAGAACAGTGACAAATGTGGGAAACATTAACTCAAAGTACAAGGCCATGGTGCAGGCTCCTCGTGGCGTAAAAATGATAGTTGAGCCTCAAACTTTAAGTTTCAATATAACCATCCAAATTCTTTCCTACAAAGTTACCTTCTTCTCAACTCAGAAAATGAATGGAGGTTACAAGTTCGGGAGCCTAACATGGACAGACGGTGTGCATCTCGTCAGGAGTCCGATAGCCGTTCGTGTGACCGGATTTGAATCATATACTGATGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 Peptidase_S8

Protein Analysis

807

Amino Acids

86.83

Weight (kDa)

8.58

Isoelectric Point (pI)

27.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S8	PF00082	3 - 41	2.1e-09	Subtilase family
Inhibitor_I9	PF05922	70 - 149	5.4e-21	Peptidase inhibitor I9
Peptidase_S8	PF00082	173 - 624	1e-45	Subtilase family
fn3_6	PF17766	701 - 795	3.6e-31	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010566)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g10480 FvH4_5g10480 FvH4_5g10480 FvH4_5g10480 FvH4_5g10480 FvH4_5g10480 FvH4_5g10480
malus_domestica	MD14G1139300.v1.1
prunus_persica	Prupe.5G133800_v2.0.a1 Prupe.5G133800_v2.0.a1
pyrus_communis	pycom14g11500
rosa_chinensis	RchiOBHm_Chr7g0187701
rosa_laevigata	RLG00000004760
rosa_multiflora	Rmu_sc0004483.1_g000024
rosa_roxburghii	Rroxscaffold_3G00267150
rosa_rugosa	Rorug06G0483600 Rorug06G0483700
rosa_samantha	Rh7AG088800 Rh7BG090500 Rh7CG089500 Rh7DG091000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1548
AccB1I	GGYRCC	2 cut(s)	1548, 1675
AccB7I	CCANNNNNTGG	1 cut(s)	459
AccI	GTMKAC	1 cut(s)	182
AciI	CCGC	3 cut(s)	797, 1673, 1709
AclWI	GGATC	8 cut(s)	239, 312, 587, 600, 1565, 1943, 1961, 1974
AcoI	YGGCCR	2 cut(s)	557, 769
AcsI	RAATTY	3 cut(s)	276, 1482, 2271
AcuI	CTGAAG	1 cut(s)	825
AfaI	GTAC	4 cut(s)	1362, 1550, 1806, 2192
AfeI	AGCGCT	1 cut(s)	699
AfiI	CCNNNNNNNGG	5 cut(s)	459, 761, 1065, 1463, 1464
AgsI	TTSAA	6 cut(s)	170, 577, 1235, 1758, 2257, 2402
AhdI	GACNNNNNGTC	1 cut(s)	748
AjnI	CCWGG	3 cut(s)	1321, 1678, 2022
Alw21I	GWGCWC	2 cut(s)	213, 679
Alw26I	GTCTC	4 cut(s)	102, 1334, 1603, 1824
Alw44I	GTGCAC	1 cut(s)	209
AlwI	GGATC	8 cut(s)	239, 312, 587, 600, 1565, 1943, 1961, 1974
AlwNI	CAGNNNCTG	2 cut(s)	566, 800
Ama87I	CYCGRG	2 cut(s)	1469, 1970
Aor51HI	AGCGCT	1 cut(s)	699
AoxI	GGCC	8 cut(s)	195, 557, 769, 948, 1099, 1258, 1897, 2196
ApaLI	GTGCAC	1 cut(s)	209
ApeKI	GCWGC	7 cut(s)	104, 308, 359, 1151, 1181, 1697, 1826
ApoI	RAATTY	3 cut(s)	276, 1482, 2271
Asp700I	GAANNNNTTC	1 cut(s)	744
Asp718I	GGTACC	1 cut(s)	1548
AspLEI	GCGC	3 cut(s)	700, 865, 2144
AspS9I	GGNCC	3 cut(s)	1100, 1631, 1898
AsuC2I	CCSGG	2 cut(s)	1971, 1972
AsuHPI	GGTGA	3 cut(s)	644, 744, 2161
AvaI	CYCGRG	2 cut(s)	1469, 1970
AvaII	GGWCC	1 cut(s)	1631
AxyI	CCTNAGG	1 cut(s)	1348
BaeGI	GKGCMC	1 cut(s)	213
BalI	TGGCCA	2 cut(s)	559, 771
BamHI	GGATCC	2 cut(s)	592, 1966
BanI	GGYRCC	2 cut(s)	1548, 1675
BanII	GRGCYC	1 cut(s)	1470
BauI	CACGAG	1 cut(s)	2214
BbsI	GAAGAC	1 cut(s)	747
Bbv12I	GWGCWC	2 cut(s)	213, 679
BbvI	GCAGC	7 cut(s)	91, 295, 346, 1163, 1193, 1684, 1838
BccI	CCATC	8 cut(s)	486, 610, 755, 953, 1091, 1457, 1868, 2273
BceAI	ACGGC	1 cut(s)	2366
BcgI	CGANNNNNNTGC	2 cut(s)	2195, 2229
BciT130I	CCWGG	3 cut(s)	1323, 1680, 2024
BcnI	CCSGG	2 cut(s)	1971, 1972
BcoDI	GTCTC	4 cut(s)	102, 1334, 1603, 1824
BfaI	CTAG	6 cut(s)	491, 836, 1626, 1635, 1844, 2049
BfmI	CTRYAG	8 cut(s)	328, 792, 826, 984, 1284, 1824, 1992, 2074
BfoI	RGCGCY	2 cut(s)	701, 866
BisI	GCNGC	9 cut(s)	105, 309, 360, 798, 1152, 1182, 1674, 1698, 1827
BlpI	GCTNAGC	1 cut(s)	1084
BlsI	GCNGC	9 cut(s)	106, 310, 361, 799, 1153, 1183, 1675, 1699, 1828
Bme1390I	CCNGG	5 cut(s)	1323, 1680, 1971, 1972, 2024
Bme18I	GGWCC	1 cut(s)	1631
BmeRI	GACNNNNNGTC	1 cut(s)	748
BmeT110I	CYCGRG	2 cut(s)	1469, 1970
BmgT120I	GGNCC	3 cut(s)	1100, 1631, 1898
BmiI	GGNNCC	8 cut(s)	594, 1045, 1550, 1632, 1677, 1968, 2211, 2336
BmrFI	CCNGG	5 cut(s)	1323, 1680, 1971, 1972, 2024
BmsI	GCATC	8 cut(s)	260, 902, 910, 1249, 1412, 2048, 2080, 2368
BpiI	GAAGAC	1 cut(s)	747
BpmI	CTGGAG	1 cut(s)	919
Bpu1102I	GCTNAGC	1 cut(s)	1084
BpuEI	CTTGAG	1 cut(s)	363
BpuMI	CCSGG	2 cut(s)	1971, 1972
BsaAI	YACGTR	1 cut(s)	1025
BsaBI	GATNNNNATC	1 cut(s)	2401
BsaI	GGTCTC	3 cut(s)	102, 1603, 1824
BsaJI	CCNNGG	8 cut(s)	755, 772, 1133, 1308, 1970, 2022, 2023, 2199
BsaWI	WCCGGW	1 cut(s)	2393
Bsc4I	CCNNNNNNNGG	5 cut(s)	459, 761, 1065, 1463, 1464
Bse1I	ACTGG	1 cut(s)	1870
Bse21I	CCTNAGG	1 cut(s)	1348
Bse3DI	GCAATG	5 cut(s)	80, 997, 1735, 1968, 2100
Bse8I	GATNNNNATC	1 cut(s)	2401
BseBI	CCWGG	3 cut(s)	1323, 1680, 2024
BseDI	CCNNGG	8 cut(s)	755, 772, 1133, 1308, 1970, 2022, 2023, 2199
BseGI	GGATG	7 cut(s)	10, 709, 766, 917, 969, 1303, 2265
BseJI	GATNNNNATC	1 cut(s)	2401
BseLI	CCNNNNNNNGG	5 cut(s)	459, 761, 1065, 1463, 1464
BseMI	GCAATG	5 cut(s)	80, 997, 1735, 1968, 2100
BseMII	CTCAG	6 cut(s)	387, 723, 1098, 1362, 1579, 2318
BseNI	ACTGG	1 cut(s)	1870
BseRI	GAGGAG	3 cut(s)	870, 873, 2202
BseSI	GKGCMC	1 cut(s)	213
BseXI	GCAGC	7 cut(s)	91, 295, 346, 1163, 1193, 1684, 1838
BsgI	GTGCAG	2 cut(s)	1113, 2225
Bsh1285I	CGRYCG	1 cut(s)	1709
BshFI	GGCC	8 cut(s)	197, 559, 771, 950, 1101, 1260, 1899, 2198
BshNI	GGYRCC	2 cut(s)	1548, 1675
BsiEI	CGRYCG	1 cut(s)	1709
BsiHKAI	GWGCWC	2 cut(s)	213, 679
BsiHKCI	CYCGRG	2 cut(s)	1469, 1970
BsiSI	CCGG	2 cut(s)	1971, 2394
BslFI	GGGAC	3 cut(s)	43, 1644, 1843
BslI	CCNNNNNNNGG	5 cut(s)	459, 761, 1065, 1463, 1464
BsmAI	GTCTC	4 cut(s)	102, 1334, 1603, 1824
BsmFI	GGGAC	3 cut(s)	43, 1644, 1843
BsmI	GAATGC	3 cut(s)	22, 432, 484
BsnI	GGCC	8 cut(s)	197, 559, 771, 950, 1101, 1260, 1899, 2198
Bso31I	GGTCTC	3 cut(s)	102, 1603, 1824
BsoBI	CYCGRG	2 cut(s)	1469, 1970
Bsp1286I	GDGCHC	3 cut(s)	213, 679, 1470
Bsp1407I	TGTACA	1 cut(s)	1804
Bsp143I	GATC	7 cut(s)	244, 317, 592, 1018, 1570, 1948, 1966
Bsp1720I	GCTNAGC	1 cut(s)	1084
Bsp19I	CCATGG	3 cut(s)	772, 1133, 2199
BspACI	CCGC	3 cut(s)	797, 1673, 1709
BspANI	GGCC	8 cut(s)	197, 559, 771, 950, 1101, 1260, 1899, 2198
BspCNI	CTCAG	6 cut(s)	388, 724, 1097, 1361, 1578, 2317
BspHI	TCATGA	1 cut(s)	955
BspLI	GGNNCC	8 cut(s)	594, 1045, 1550, 1632, 1677, 1968, 2211, 2336
BspMAI	CTGCAG	3 cut(s)	1828, 1996, 2078
BspPI	GGATC	8 cut(s)	239, 312, 587, 600, 1565, 1943, 1961, 1974
BspT107I	GGYRCC	2 cut(s)	1548, 1675
BspTNI	GGTCTC	3 cut(s)	102, 1603, 1824
BsrDI	GCAATG	5 cut(s)	80, 997, 1735, 1968, 2100
BsrGI	TGTACA	1 cut(s)	1804
BsrI	ACTGG	1 cut(s)	1870
BssECI	CCNNGG	8 cut(s)	755, 772, 1133, 1308, 1970, 2022, 2023, 2199
BssMI	GATC	7 cut(s)	244, 317, 592, 1018, 1570, 1948, 1966
BssNAI	GTATAC	1 cut(s)	183
BssSI	CACGAG	1 cut(s)	2214
BssT1I	CCWWGG	4 cut(s)	772, 1133, 1308, 2199
Bst1107I	GTATAC	1 cut(s)	183
Bst2BI	CACGAG	1 cut(s)	2214
Bst2UI	CCWGG	3 cut(s)	1323, 1680, 2024
Bst4CI	ACNGT	8 cut(s)	1075, 1147, 1414, 1582, 2033, 2149, 2161, 2354
BstAUI	TGTACA	1 cut(s)	1804
BstBAI	YACGTR	1 cut(s)	1025
BstC8I	GCNNGC	8 cut(s)	20, 58, 626, 679, 802, 895, 1258, 2208
BstDEI	CTNAG	7 cut(s)	396, 732, 877, 1084, 1348, 1565, 2304
BstDSI	CCRYGG	3 cut(s)	772, 1133, 2199
BstF5I	GGATG	7 cut(s)	10, 709, 766, 917, 969, 1303, 2265
BstH2I	RGCGCY	2 cut(s)	701, 866
BstHHI	GCGC	3 cut(s)	700, 865, 2144
BstKTI	GATC	7 cut(s)	247, 320, 595, 1021, 1573, 1951, 1969
BstMAI	GTCTC	4 cut(s)	102, 1334, 1603, 1824
BstMBI	GATC	7 cut(s)	244, 317, 592, 1018, 1570, 1948, 1966
BstMCI	CGRYCG	1 cut(s)	1709
BstMWI	GCNNNNNNNGC	7 cut(s)	634, 794, 869, 878, 1178, 1343, 1905
BstNI	CCWGG	3 cut(s)	1323, 1680, 2024
BstNSI	RCATGY	2 cut(s)	1767, 1946
BstSCI	CCNGG	5 cut(s)	1321, 1678, 1969, 1970, 2022
BstSFI	CTRYAG	8 cut(s)	328, 792, 826, 984, 1284, 1824, 1992, 2074
BstSLI	GKGCMC	1 cut(s)	213
BstV1I	GCAGC	7 cut(s)	91, 295, 346, 1163, 1193, 1684, 1838
BstV2I	GAAGAC	1 cut(s)	747
BstX2I	RGATCY	3 cut(s)	317, 592, 1966
BstXI	CCANNNNNNTGG	1 cut(s)	609
BstYI	RGATCY	3 cut(s)	317, 592, 1966
BstZ17I	GTATAC	1 cut(s)	183
Bsu36I	CCTNAGG	1 cut(s)	1348
BsuRI	GGCC	8 cut(s)	197, 559, 771, 950, 1101, 1260, 1899, 2198
BtgI	CCRYGG	3 cut(s)	772, 1133, 2199
BtsCI	GGATG	7 cut(s)	10, 709, 766, 917, 969, 1303, 2265
BtsI	GCAGTG	2 cut(s)	1125, 1739
BtsIMutI	CAGTG	6 cut(s)	915, 1125, 1143, 1573, 1739, 2166
Cac8I	GCNNGC	8 cut(s)	20, 58, 626, 679, 802, 895, 1258, 2208
CaiI	CAGNNNCTG	2 cut(s)	566, 800
CciI	TCATGA	1 cut(s)	955
CfoI	GCGC	3 cut(s)	700, 865, 2144
Cfr13I	GGNCC	3 cut(s)	1100, 1631, 1898
Cfr9I	CCCGGG	1 cut(s)	1970
CseI	GACGC	1 cut(s)	1845
Csp6I	GTAC	4 cut(s)	1361, 1549, 1805, 2191
CspCI	CAANNNNNGTGG	2 cut(s)	1567, 1602
CviQI	GTAC	4 cut(s)	1361, 1549, 1805, 2191
DdeI	CTNAG	7 cut(s)	396, 732, 877, 1084, 1348, 1565, 2304
DpnI	GATC	7 cut(s)	246, 319, 594, 1020, 1572, 1950, 1968
DpnII	GATC	7 cut(s)	244, 317, 592, 1018, 1570, 1948, 1966
DraI	TTTAAA	2 cut(s)	75, 1659
DriI	GACNNNNNGTC	1 cut(s)	748
EaeI	YGGCCR	2 cut(s)	557, 769
Eam1105I	GACNNNNNGTC	1 cut(s)	748
Eco130I	CCWWGG	4 cut(s)	772, 1133, 1308, 2199
Eco147I	AGGCCT	1 cut(s)	1260
Eco24I	GRGCYC	1 cut(s)	1470
Eco31I	GGTCTC	3 cut(s)	102, 1603, 1824
Eco47I	GGWCC	1 cut(s)	1631
Eco47III	AGCGCT	1 cut(s)	699
Eco57I	CTGAAG	1 cut(s)	825
Eco81I	CCTNAGG	1 cut(s)	1348
Eco88I	CYCGRG	2 cut(s)	1469, 1970
EcoO109I	RGGNCCY	1 cut(s)	1631
EcoRII	CCWGG	3 cut(s)	1321, 1678, 2022
EcoT14I	CCWWGG	4 cut(s)	772, 1133, 1308, 2199
EcoT22I	ATGCAT	1 cut(s)	1493
EcoT38I	GRGCYC	1 cut(s)	1470
ErhI	CCWWGG	4 cut(s)	772, 1133, 1308, 2199
FalI	AAGNNNNNCTT	2 cut(s)	329, 361
FaqI	GGGAC	3 cut(s)	43, 1644, 1843
FblI	GTMKAC	1 cut(s)	182
Fnu4HI	GCNGC	9 cut(s)	105, 309, 360, 798, 1152, 1182, 1674, 1698, 1827
FokI	GGATG	7 cut(s)	17, 716, 773, 924, 956, 1310, 2252
FriOI	GRGCYC	1 cut(s)	1470
Fsp4HI	GCNGC	9 cut(s)	105, 309, 360, 798, 1152, 1182, 1674, 1698, 1827
FspBI	CTAG	6 cut(s)	491, 836, 1626, 1635, 1844, 2049
GlaI	GCGC	3 cut(s)	699, 864, 2143
GluI	GCNGC	9 cut(s)	105, 309, 360, 798, 1152, 1182, 1674, 1698, 1827
GsuI	CTGGAG	1 cut(s)	919
HaeII	RGCGCY	2 cut(s)	701, 866
HaeIII	GGCC	8 cut(s)	197, 559, 771, 950, 1101, 1260, 1899, 2198
HapII	CCGG	2 cut(s)	1971, 2394
HgaI	GACGC	1 cut(s)	1845
HhaI	GCGC	3 cut(s)	700, 865, 2144
Hin6I	GCGC	3 cut(s)	698, 863, 2142
HinP1I	GCGC	3 cut(s)	698, 863, 2142
HpaII	CCGG	2 cut(s)	1971, 2394
HphI	GGTGA	3 cut(s)	644, 744, 2161
Hpy166II	GTNNAC	5 cut(s)	183, 211, 967, 1123, 1687
Hpy188I	TCNGA	8 cut(s)	282, 473, 568, 1273, 1381, 1538, 2307, 2376
Hpy188III	TCNNGA	9 cut(s)	351, 404, 491, 843, 956, 1749, 1811, 2332, 2368
Hpy8I	GTNNAC	5 cut(s)	183, 211, 967, 1123, 1687
HpyAV	CCTTC	6 cut(s)	578, 1057, 1057, 1889, 2121, 2302
HpyCH4III	ACNGT	8 cut(s)	1075, 1147, 1414, 1582, 2033, 2149, 2161, 2354
HpyCH4IV	ACGT	1 cut(s)	1024
HpyF10VI	GCNNNNNNNGC	7 cut(s)	634, 794, 869, 878, 1178, 1343, 1905
HpyF3I	CTNAG	7 cut(s)	396, 732, 877, 1084, 1348, 1565, 2304
HpySE526I	ACGT	1 cut(s)	1024
HspAI	GCGC	3 cut(s)	698, 863, 2142
KpnI	GGTACC	1 cut(s)	1552
Kzo9I	GATC	7 cut(s)	244, 317, 592, 1018, 1570, 1948, 1966
LmnI	GCTCC	7 cut(s)	447, 695, 860, 900, 2024, 2215, 2334
Lsp1109I	GCAGC	7 cut(s)	91, 295, 346, 1163, 1193, 1684, 1838
LweI	GCATC	8 cut(s)	260, 902, 910, 1249, 1412, 2048, 2080, 2368
MaeI	CTAG	6 cut(s)	491, 836, 1626, 1635, 1844, 2049
MaeII	ACGT	1 cut(s)	1024
MaeIII	GTNAC	9 cut(s)	750, 1774, 1846, 2027, 2149, 2161, 2287, 2321, 2389
MalI	GATC	7 cut(s)	246, 319, 594, 1020, 1572, 1950, 1968
MboI	GATC	7 cut(s)	244, 317, 592, 1018, 1570, 1948, 1966
MboII	GAAGA	3 cut(s)	752, 2091, 2287
MfeI	CAATTG	4 cut(s)	390, 1032, 1038, 1554
MflI	RGATCY	3 cut(s)	317, 592, 1966
MhlI	GDGCHC	3 cut(s)	213, 679, 1470
MlsI	TGGCCA	2 cut(s)	559, 771
MluNI	TGGCCA	2 cut(s)	559, 771
MlyI	GAGTC	4 cut(s)	572, 1201, 2092, 2380
MmeI	TCCRAC	2 cut(s)	1567, 1598
Mox20I	TGGCCA	2 cut(s)	559, 771
Mph1103I	ATGCAT	1 cut(s)	1493
MroXI	GAANNNNTTC	1 cut(s)	744
MscI	TGGCCA	2 cut(s)	559, 771
MslI	CAYNNNNRTG	1 cut(s)	1245
Msp20I	TGGCCA	2 cut(s)	559, 771
MspA1I	CMGCKG	2 cut(s)	797, 1088
MspI	CCGG	2 cut(s)	1971, 2394
MspR9I	CCNGG	5 cut(s)	1323, 1680, 1971, 1972, 2024
MunI	CAATTG	4 cut(s)	390, 1032, 1038, 1554
Mva1269I	GAATGC	3 cut(s)	22, 432, 484
MvaI	CCWGG	3 cut(s)	1323, 1680, 2024
MwoI	GCNNNNNNNGC	7 cut(s)	634, 794, 869, 878, 1178, 1343, 1905
NciI	CCSGG	2 cut(s)	1971, 1972
NcoI	CCATGG	3 cut(s)	772, 1133, 2199
NdeII	GATC	7 cut(s)	244, 317, 592, 1018, 1570, 1948, 1966
NlaIV	GGNNCC	8 cut(s)	594, 1045, 1550, 1632, 1677, 1968, 2211, 2336
NmuCI	GTSAC	6 cut(s)	750, 1774, 1846, 2149, 2161, 2389
NsiI	ATGCAT	1 cut(s)	1493
NspI	RCATGY	2 cut(s)	1767, 1946
PaeR7I	CTCGAG	1 cut(s)	1469
PagI	TCATGA	1 cut(s)	955
PasI	CCCWGGG	1 cut(s)	2023
PceI	AGGCCT	1 cut(s)	1260
PctI	GAATGC	3 cut(s)	22, 432, 484
PdmI	GAANNNNTTC	1 cut(s)	744
PfeI	GAWTC	9 cut(s)	12, 374, 421, 474, 545, 707, 997, 1301, 2402
PflMI	CCANNNNNTGG	1 cut(s)	459
PkrI	GCNGC	9 cut(s)	106, 310, 361, 799, 1153, 1183, 1675, 1699, 1828
PleI	GAGTC	4 cut(s)	571, 1201, 2092, 2379
PpsI	GAGTC	4 cut(s)	571, 1201, 2092, 2379
Ppu21I	YACGTR	1 cut(s)	1025
PpuMI	RGGWCCY	1 cut(s)	1631
Psp5II	RGGWCCY	1 cut(s)	1631
Psp6I	CCWGG	3 cut(s)	1321, 1678, 2022
PspGI	CCWGG	3 cut(s)	1321, 1678, 2022
PspN4I	GGNNCC	8 cut(s)	594, 1045, 1550, 1632, 1677, 1968, 2211, 2336
PspPI	GGNCC	3 cut(s)	1100, 1631, 1898
PspPPI	RGGWCCY	1 cut(s)	1631
PspXI	VCTCGAGB	1 cut(s)	1469
PstI	CTGCAG	3 cut(s)	1828, 1996, 2078
PstNI	CAGNNNCTG	2 cut(s)	566, 800
PsuI	RGATCY	3 cut(s)	317, 592, 1966
PvuII	CAGCTG	1 cut(s)	1088
RsaI	GTAC	4 cut(s)	1362, 1550, 1806, 2192
RsaNI	GTAC	4 cut(s)	1361, 1549, 1805, 2191
RseI	CAYNNNNRTG	1 cut(s)	1245
SatI	GCNGC	9 cut(s)	105, 309, 360, 798, 1152, 1182, 1674, 1698, 1827
Sau3AI	GATC	7 cut(s)	244, 317, 592, 1018, 1570, 1948, 1966
Sau96I	GGNCC	3 cut(s)	1100, 1631, 1898
SchI	GAGTC	4 cut(s)	572, 1201, 2092, 2380
ScrFI	CCNGG	5 cut(s)	1323, 1680, 1971, 1972, 2024
SduI	GDGCHC	3 cut(s)	213, 679, 1470
SfaNI	GCATC	8 cut(s)	260, 902, 910, 1249, 1412, 2048, 2080, 2368
SfcI	CTRYAG	8 cut(s)	328, 792, 826, 984, 1284, 1824, 1992, 2074
Sfr274I	CTCGAG	1 cut(s)	1469
SinI	GGWCC	1 cut(s)	1631
SlaI	CTCGAG	1 cut(s)	1469
SmaI	CCCGGG	1 cut(s)	1972
SmiMI	CAYNNNNRTG	1 cut(s)	1245
SmlI	CTYRAG	2 cut(s)	378, 1469
SmoI	CTYRAG	2 cut(s)	378, 1469
SseBI	AGGCCT	1 cut(s)	1260
SsiI	CCGC	3 cut(s)	797, 1673, 1709
SspMI	CTAG	6 cut(s)	491, 836, 1626, 1635, 1844, 2049
StuI	AGGCCT	1 cut(s)	1260
StyD4I	CCNGG	5 cut(s)	1321, 1678, 1969, 1970, 2022
StyI	CCWWGG	4 cut(s)	772, 1133, 1308, 2199
TaaI	ACNGT	8 cut(s)	1075, 1147, 1414, 1582, 2033, 2149, 2161, 2354
TaiI	ACGT	1 cut(s)	1027
TaqI	TCGA	4 cut(s)	192, 1017, 1470, 1705
TatI	WGTACW	3 cut(s)	1360, 1804, 2190
TauI	GCSGC	2 cut(s)	800, 1676
TfiI	GAWTC	9 cut(s)	12, 374, 421, 474, 545, 707, 997, 1301, 2402
TscAI	CASTG	6 cut(s)	922, 1132, 1150, 1573, 1746, 2166
TseFI	GTSAC	6 cut(s)	750, 1774, 1846, 2149, 2161, 2389
TseI	GCWGC	7 cut(s)	104, 308, 359, 1151, 1181, 1697, 1826
Tsp45I	GTSAC	6 cut(s)	750, 1774, 1846, 2149, 2161, 2389
TspDTI	ATGAA	9 cut(s)	145, 257, 457, 720, 944, 1076, 1398, 1521, 2327
TspMI	CCCGGG	1 cut(s)	1970
TspRI	CASTG	6 cut(s)	922, 1132, 1150, 1573, 1746, 2166
Van91I	CCANNNNNTGG	1 cut(s)	459
VneI	GTGCAC	1 cut(s)	209
VpaK11BI	GGWCC	1 cut(s)	1631
XapI	RAATTY	3 cut(s)	276, 1482, 2271
XbaI	TCTAGA	1 cut(s)	490
XceI	RCATGY	2 cut(s)	1767, 1946
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1960
XhoI	CTCGAG	1 cut(s)	1469
XmaI	CCCGGG	1 cut(s)	1970
XmiI	GTMKAC	1 cut(s)	182
XmnI	GAANNNNTTC	1 cut(s)	744
XspI	CTAG	6 cut(s)	491, 836, 1626, 1635, 1844, 2049
Zsp2I	ATGCAT	1 cut(s)	1493

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.