Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7B

Orthogroup ID

OG0010566

Physical Location & Seq

Genomic Coordinates Forward (+)

6692387 .. 6695500

Gene Structure 3114 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7BG090500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2337 bp

ATGTTGATGGATTTCAATACAAGAAACGGGACTTGTGCAGGAAAGAACTACTCTCTGGTGGTTCTTGCTTTCATCTTATTACTTCAACATCATCTGTATACATTAGTCGAGGCCACAAGTAAAGTGCACATTGTATATTTGGGAGAAAAGAAACATGATGATCCAGCATCTACAAAGAAGTTTCATCATAAAATTCTGACCAACTTGTTAGGAAGCAAAGAAGCTGCATACAGCTCCATTCTCTATAGCTATAAGCATGGCTTTTCTGGATTTGCTGCAAGATTAACAGAATCTCAAGCAGAAACAATTGCTGAGTTTCCAGGAGTTGTCCAAGTGATTCCCAATCGCATTCACAAGCTCCATACAACCAGAAGTTGGGATTTCATCGGAATCCATCAGCATTCTCCAGAAAATCATTTAACAAGAAGCATGGGCAAAGGAACTATAATTGGAGTAATAGATTCAGGTGTTTGGCCAGAGTCTGAAAGTTTCAAGGACGAAGGCATGGATCCTATCCCATCACATTGGAAAGGAATTTGCCAGCAGGGTGAGCTTTTTAACTCCACAAATTGCAATAAGAAACTTATTGGTGCTCGCTGGTTTGTAAAAGGAGCGCTGGATGAATCCAAAACACCAATTAACAAAACTGAGGGAGAAGACTTTCTGTCACCGAGGGATGGAATTGGCCATGGCACTCATACAGCTTCTACAGCGGCTGGCTATTTTGTCAAAAGAGCCAACTATACAGGACTAGCTTCAGGATTAGCCAGAGGAGGAGCGCCTCTTGCTCACTTAGCAATTTACAAGGCTTGCTGGAGCAATAGAGGATGCACTGATGCTGATATTCTTAAAGCATTTGACAAGGCCATTCATGATGGAGTGGACATCCTATCTGTTTCTGTAGGCAATGAGATTCCTTTGTTCTCTTATGTCGATCTACGTGATACAATTGCAATTGGTTCCTTCCATGCAACCATGAAGGGAATTACAGTTGTTTGCTCAGCTGGAAATGATGGCCCTATCTCGCACACTATTGTAAACACTGCACCATGGCTCATCACTGTTGCAGCCACCAAAATAGACAGGGCATTTCCAGCAGCCATTACACTTGGAAATAACCAGACTCTTTGGGGTCAATCTATTGATATTGAAAAGCATCACCATGGATTTGCAGGCCTCACATACTCTGAACGCATAGCTATAGACCCTACAGATGATTCAGCCAAGGATTGTCAGCCTGGAAGTCTCAATGCAACATTAGCCTCAGGGAAAATAGTACTCTGTTTCTCCATATCAGATGAACAAGACATAGTTTCTGCATCAGCTACCGTGAAAAAGGCTGGTGGAATTGGACTAATATATGCACAGTTTCCCAATGATGGGCTCGAGCCATGCAAAATTCCATGCATTAAAGTAGATTATGAAGTAGGAACACAAATACTTGTCTACATCAGAAAAGCAAGGTACCCAATTGCCAAGCTCAGTGATCCAATCACCGTTGTTGGAAAATGGGTCTCTCCACAAGTTGCTACTTTCTCATCTAGAGGACCTAGTTCCATGACACCAGCAGTTTTAAAGCCTGACATAGCGGCACCCGGCGTGGACATCATAGCTGCATTTAAACCGCATGAGAAAAAACAAAGCAATGGTTATGCACTGCTATCAGGAACTTCAATGGCATGTCCTCATGTGACAGGGATTGTAGCTCTCATCAAATCTGTACATCAAGATTGGTCTCCTGCAGCAATAAAATCAGCTCTAGTCACTACTGCTTCCCAGACTGGAACAGATGGAACAAGCATTTCTGCCGAAGGCCCAACTCGCAAGGAAGCTGACCCATTTGACATAGGTGGTGGGCAAGTTGATCCCAACAAGGCAATGGATCCCGGGCTAATTTACAATGCTACTGCAGACGACTACATTCAGTTTCTCTGCTCCCTGGGTTACAGTAGTGCATCACTTACTAGATTAACCAAGACTACAATAAACTGCATAAGAAAAACTGATGCAATGAATCTCAACCTCCCATCTATCGCAATTCCAAACCTGAAAAAGACCGCGACGGTGAGAAGAACAGTGACAAATGTGGGAAACATTAACTCAAAGTACAAGGCCGTGGTGCAGGCTCCTCGTGGCCTAAAAATGACAGTTGAGCCTCAAACTTTAAGTTTCAATATAACCACCCAAATTCTTTCCTACAAAGTTACCTTCTTCTCAACTCAGAAAATGAATGGAGATTACAAGTTCGGGAGCCTAACATGGACAGACGGTGAGCATCTCGTCAGGAGTCCAATAGCCGTTCGTGTGACCCGATTTGAATCATATACTGATGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 Peptidase_S8

Protein Analysis

778

Amino Acids

84.08

Weight (kDa)

8.64

Isoelectric Point (pI)

25.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	42 - 121	5e-21	Peptidase inhibitor I9
Peptidase_S8	PF00082	145 - 596	7.4e-46	Subtilase family
fn3_6	PF17766	672 - 767	2.8e-32	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010566)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g10480 FvH4_5g10480 FvH4_5g10480 FvH4_5g10480 FvH4_5g10480 FvH4_5g10480 FvH4_5g10480
malus_domestica	MD14G1139300.v1.1
prunus_persica	Prupe.5G133800_v2.0.a1 Prupe.5G133800_v2.0.a1
pyrus_communis	pycom14g11500
rosa_chinensis	RchiOBHm_Chr7g0187701
rosa_laevigata	RLG00000004760
rosa_multiflora	Rmu_sc0004483.1_g000024
rosa_roxburghii	Rroxscaffold_3G00267150
rosa_rugosa	Rorug06G0483600 Rorug06G0483700
rosa_samantha	Rh7AG088800 Rh7BG090500 Rh7CG089500 Rh7DG091000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1464
AccB1I	GGYRCC	2 cut(s)	1464, 1591
AccB7I	CCANNNNNTGG	1 cut(s)	375
AccI	GTMKAC	2 cut(s)	98, 1446
AccII	CGCG	1 cut(s)	2060
AciI	CCGC	4 cut(s)	713, 1589, 1625, 2058
AclWI	GGATC	7 cut(s)	155, 503, 516, 1481, 1859, 1877, 1890
AcoI	YGGCCR	2 cut(s)	473, 685
AcsI	RAATTY	4 cut(s)	192, 534, 1398, 2187
AcuI	CTGAAG	1 cut(s)	741
AfaI	GTAC	4 cut(s)	1278, 1466, 1722, 2108
AfeI	AGCGCT	1 cut(s)	615
AfiI	CCNNNNNNNGG	4 cut(s)	375, 677, 1379, 1380
AgsI	TTSAA	7 cut(s)	16, 86, 493, 1151, 1674, 2173, 2318
AhdI	GACNNNNNGTC	1 cut(s)	664
AjnI	CCWGG	3 cut(s)	319, 1237, 1938
Alw21I	GWGCWC	2 cut(s)	129, 595
Alw26I	GTCTC	3 cut(s)	1250, 1519, 1740
Alw44I	GTGCAC	1 cut(s)	125
AlwI	GGATC	7 cut(s)	155, 503, 516, 1481, 1859, 1877, 1890
AlwNI	CAGNNNCTG	2 cut(s)	482, 716
Ama87I	CYCGRG	2 cut(s)	1385, 1886
Aor51HI	AGCGCT	1 cut(s)	615
AoxI	GGCC	9 cut(s)	111, 473, 685, 864, 1015, 1174, 1813, 2112, 2134
ApaLI	GTGCAC	1 cut(s)	125
ApeKI	GCWGC	6 cut(s)	224, 275, 1067, 1097, 1613, 1742
ApoI	RAATTY	4 cut(s)	192, 534, 1398, 2187
Asp700I	GAANNNNTTC	1 cut(s)	660
Asp718I	GGTACC	1 cut(s)	1464
AspLEI	GCGC	2 cut(s)	616, 781
AspS9I	GGNCC	3 cut(s)	1016, 1547, 1814
AsuC2I	CCSGG	3 cut(s)	1596, 1887, 1888
AsuHPI	GGTGA	6 cut(s)	560, 660, 1151, 1486, 2077, 2282
AvaI	CYCGRG	2 cut(s)	1385, 1886
AvaII	GGWCC	1 cut(s)	1547
AxyI	CCTNAGG	1 cut(s)	1264
BaeGI	GKGCMC	1 cut(s)	129
BalI	TGGCCA	2 cut(s)	475, 687
BamHI	GGATCC	2 cut(s)	508, 1882
BanI	GGYRCC	2 cut(s)	1464, 1591
BanII	GRGCYC	1 cut(s)	1386
BauI	CACGAG	1 cut(s)	2130
BbsI	GAAGAC	1 cut(s)	663
Bbv12I	GWGCWC	2 cut(s)	129, 595
BbvI	GCAGC	6 cut(s)	211, 262, 1079, 1109, 1600, 1754
BccI	CCATC	8 cut(s)	402, 526, 671, 869, 1007, 1373, 1784, 2035
BceAI	ACGGC	2 cut(s)	2099, 2282
BcgI	CGANNNNNNTGC	2 cut(s)	2111, 2145
BciT130I	CCWGG	3 cut(s)	321, 1239, 1940
BcnI	CCSGG	3 cut(s)	1596, 1887, 1888
BcoDI	GTCTC	3 cut(s)	1250, 1519, 1740
BfaI	CTAG	5 cut(s)	752, 1542, 1551, 1760, 1965
BfmI	CTRYAG	7 cut(s)	244, 708, 900, 1200, 1209, 1740, 1908
BfoI	RGCGCY	2 cut(s)	617, 782
BisI	GCNGC	8 cut(s)	225, 276, 714, 1068, 1098, 1590, 1614, 1743
BlpI	GCTNAGC	1 cut(s)	1000
BlsI	GCNGC	8 cut(s)	226, 277, 715, 1069, 1099, 1591, 1615, 1744
BmcAI	AGTACT	1 cut(s)	1278
Bme1390I	CCNGG	6 cut(s)	321, 1239, 1596, 1887, 1888, 1940
Bme18I	GGWCC	1 cut(s)	1547
BmeRI	GACNNNNNGTC	1 cut(s)	664
BmeT110I	CYCGRG	2 cut(s)	1385, 1886
BmgT120I	GGNCC	3 cut(s)	1016, 1547, 1814
BmiI	GGNNCC	7 cut(s)	510, 961, 1466, 1593, 1884, 2127, 2252
BmrFI	CCNGG	6 cut(s)	321, 1239, 1596, 1887, 1888, 1940
BmsI	GCATC	8 cut(s)	176, 818, 826, 1165, 1328, 1964, 1996, 2284
BpiI	GAAGAC	1 cut(s)	663
BpmI	CTGGAG	2 cut(s)	390, 835
Bpu1102I	GCTNAGC	1 cut(s)	1000
BpuEI	CTTGAG	1 cut(s)	279
BpuMI	CCSGG	3 cut(s)	1596, 1887, 1888
BsaAI	YACGTR	1 cut(s)	941
BsaBI	GATNNNNATC	1 cut(s)	2317
BsaI	GGTCTC	2 cut(s)	1519, 1740
BsaJI	CCNNGG	9 cut(s)	671, 688, 1049, 1162, 1224, 1886, 1938, 1939, 2115
Bsc4I	CCNNNNNNNGG	4 cut(s)	375, 677, 1379, 1380
Bse1I	ACTGG	1 cut(s)	1786
Bse21I	CCTNAGG	1 cut(s)	1264
Bse3DI	GCAATG	4 cut(s)	913, 1651, 1884, 2016
Bse8I	GATNNNNATC	1 cut(s)	2317
BseBI	CCWGG	3 cut(s)	321, 1239, 1940
BseDI	CCNNGG	9 cut(s)	671, 688, 1049, 1162, 1224, 1886, 1938, 1939, 2115
BseGI	GGATG	4 cut(s)	625, 682, 833, 885
BseJI	GATNNNNATC	1 cut(s)	2317
BseLI	CCNNNNNNNGG	4 cut(s)	375, 677, 1379, 1380
BseMI	GCAATG	4 cut(s)	913, 1651, 1884, 2016
BseMII	CTCAG	6 cut(s)	303, 639, 1014, 1278, 1495, 2234
BseNI	ACTGG	1 cut(s)	1786
BseRI	GAGGAG	3 cut(s)	786, 789, 2118
BseSI	GKGCMC	1 cut(s)	129
BseXI	GCAGC	6 cut(s)	211, 262, 1079, 1109, 1600, 1754
BsgI	GTGCAG	3 cut(s)	57, 1029, 2141
Bsh1236I	CGCG	1 cut(s)	2060
BshFI	GGCC	9 cut(s)	113, 475, 687, 866, 1017, 1176, 1815, 2114, 2136
BshNI	GGYRCC	2 cut(s)	1464, 1591
BsiHKAI	GWGCWC	2 cut(s)	129, 595
BsiHKCI	CYCGRG	2 cut(s)	1385, 1886
BsiSI	CCGG	2 cut(s)	1596, 1887
BslFI	GGGAC	1 cut(s)	43
BslI	CCNNNNNNNGG	4 cut(s)	375, 677, 1379, 1380
BsmAI	GTCTC	3 cut(s)	1250, 1519, 1740
BsmFI	GGGAC	1 cut(s)	43
BsmI	GAATGC	2 cut(s)	348, 400
BsnI	GGCC	9 cut(s)	113, 475, 687, 866, 1017, 1176, 1815, 2114, 2136
Bso31I	GGTCTC	2 cut(s)	1519, 1740
BsoBI	CYCGRG	2 cut(s)	1385, 1886
Bsp1286I	GDGCHC	3 cut(s)	129, 595, 1386
Bsp1407I	TGTACA	1 cut(s)	1720
Bsp143I	GATC	6 cut(s)	160, 508, 934, 1486, 1864, 1882
Bsp1720I	GCTNAGC	1 cut(s)	1000
Bsp19I	CCATGG	3 cut(s)	688, 1049, 1162
BspACI	CCGC	4 cut(s)	713, 1589, 1625, 2058
BspANI	GGCC	9 cut(s)	113, 475, 687, 866, 1017, 1176, 1815, 2114, 2136
BspCNI	CTCAG	6 cut(s)	304, 640, 1013, 1277, 1494, 2233
BspFNI	CGCG	1 cut(s)	2060
BspHI	TCATGA	1 cut(s)	871
BspLI	GGNNCC	7 cut(s)	510, 961, 1466, 1593, 1884, 2127, 2252
BspMAI	CTGCAG	2 cut(s)	1744, 1912
BspPI	GGATC	7 cut(s)	155, 503, 516, 1481, 1859, 1877, 1890
BspT107I	GGYRCC	2 cut(s)	1464, 1591
BspTNI	GGTCTC	2 cut(s)	1519, 1740
BsrDI	GCAATG	4 cut(s)	913, 1651, 1884, 2016
BsrGI	TGTACA	1 cut(s)	1720
BsrI	ACTGG	1 cut(s)	1786
BssECI	CCNNGG	9 cut(s)	671, 688, 1049, 1162, 1224, 1886, 1938, 1939, 2115
BssMI	GATC	6 cut(s)	160, 508, 934, 1486, 1864, 1882
BssNAI	GTATAC	1 cut(s)	99
BssSI	CACGAG	1 cut(s)	2130
BssT1I	CCWWGG	4 cut(s)	688, 1049, 1162, 1224
Bst1107I	GTATAC	1 cut(s)	99
Bst2BI	CACGAG	1 cut(s)	2130
Bst2UI	CCWGG	3 cut(s)	321, 1239, 1940
BstAUI	TGTACA	1 cut(s)	1720
BstBAI	YACGTR	1 cut(s)	941
BstC8I	GCNNGC	6 cut(s)	542, 595, 718, 811, 1174, 2124
BstDEI	CTNAG	7 cut(s)	312, 648, 793, 1000, 1264, 1481, 2220
BstDSI	CCRYGG	4 cut(s)	688, 1049, 1162, 2115
BstF5I	GGATG	4 cut(s)	625, 682, 833, 885
BstFNI	CGCG	1 cut(s)	2060
BstH2I	RGCGCY	2 cut(s)	617, 782
BstHHI	GCGC	2 cut(s)	616, 781
BstKTI	GATC	6 cut(s)	163, 511, 937, 1489, 1867, 1885
BstMAI	GTCTC	3 cut(s)	1250, 1519, 1740
BstMBI	GATC	6 cut(s)	160, 508, 934, 1486, 1864, 1882
BstMWI	GCNNNNNNNGC	7 cut(s)	550, 710, 785, 794, 1094, 1259, 1821
BstNI	CCWGG	3 cut(s)	321, 1239, 1940
BstNSI	RCATGY	1 cut(s)	1683
BstSCI	CCNGG	6 cut(s)	319, 1237, 1594, 1885, 1886, 1938
BstSFI	CTRYAG	7 cut(s)	244, 708, 900, 1200, 1209, 1740, 1908
BstSLI	GKGCMC	1 cut(s)	129
BstUI	CGCG	1 cut(s)	2060
BstV1I	GCAGC	6 cut(s)	211, 262, 1079, 1109, 1600, 1754
BstV2I	GAAGAC	1 cut(s)	663
BstX2I	RGATCY	2 cut(s)	508, 1882
BstXI	CCANNNNNNTGG	1 cut(s)	525
BstYI	RGATCY	2 cut(s)	508, 1882
BstZ17I	GTATAC	1 cut(s)	99
Bsu36I	CCTNAGG	1 cut(s)	1264
BsuRI	GGCC	9 cut(s)	113, 475, 687, 866, 1017, 1176, 1815, 2114, 2136
BtgI	CCRYGG	4 cut(s)	688, 1049, 1162, 2115
BtsCI	GGATG	4 cut(s)	625, 682, 833, 885
BtsI	GCAGTG	2 cut(s)	1041, 1655
BtsIMutI	CAGTG	6 cut(s)	831, 1041, 1059, 1489, 1655, 2082
Cac8I	GCNNGC	6 cut(s)	542, 595, 718, 811, 1174, 2124
CaiI	CAGNNNCTG	2 cut(s)	482, 716
CciI	TCATGA	1 cut(s)	871
CfoI	GCGC	2 cut(s)	616, 781
Cfr13I	GGNCC	3 cut(s)	1016, 1547, 1814
Cfr9I	CCCGGG	1 cut(s)	1886
Csp6I	GTAC	4 cut(s)	1277, 1465, 1721, 2107
CviQI	GTAC	4 cut(s)	1277, 1465, 1721, 2107
DdeI	CTNAG	7 cut(s)	312, 648, 793, 1000, 1264, 1481, 2220
DpnI	GATC	6 cut(s)	162, 510, 936, 1488, 1866, 1884
DpnII	GATC	6 cut(s)	160, 508, 934, 1486, 1864, 1882
DraI	TTTAAA	2 cut(s)	1575, 1621
DriI	GACNNNNNGTC	1 cut(s)	664
EaeI	YGGCCR	2 cut(s)	473, 685
Eam1105I	GACNNNNNGTC	1 cut(s)	664
Eco130I	CCWWGG	4 cut(s)	688, 1049, 1162, 1224
Eco147I	AGGCCT	1 cut(s)	1176
Eco24I	GRGCYC	1 cut(s)	1386
Eco31I	GGTCTC	2 cut(s)	1519, 1740
Eco47I	GGWCC	1 cut(s)	1547
Eco47III	AGCGCT	1 cut(s)	615
Eco57I	CTGAAG	1 cut(s)	741
Eco81I	CCTNAGG	1 cut(s)	1264
Eco88I	CYCGRG	2 cut(s)	1385, 1886
EcoO109I	RGGNCCY	1 cut(s)	1547
EcoRII	CCWGG	3 cut(s)	319, 1237, 1938
EcoT14I	CCWWGG	4 cut(s)	688, 1049, 1162, 1224
EcoT22I	ATGCAT	1 cut(s)	1409
EcoT38I	GRGCYC	1 cut(s)	1386
ErhI	CCWWGG	4 cut(s)	688, 1049, 1162, 1224
FalI	AAGNNNNNCTT	2 cut(s)	245, 277
FaqI	GGGAC	1 cut(s)	43
FblI	GTMKAC	2 cut(s)	98, 1446
Fnu4HI	GCNGC	8 cut(s)	225, 276, 714, 1068, 1098, 1590, 1614, 1743
FokI	GGATG	4 cut(s)	632, 689, 840, 872
FriOI	GRGCYC	1 cut(s)	1386
Fsp4HI	GCNGC	8 cut(s)	225, 276, 714, 1068, 1098, 1590, 1614, 1743
FspBI	CTAG	5 cut(s)	752, 1542, 1551, 1760, 1965
GlaI	GCGC	2 cut(s)	615, 780
GluI	GCNGC	8 cut(s)	225, 276, 714, 1068, 1098, 1590, 1614, 1743
GsuI	CTGGAG	2 cut(s)	390, 835
HaeII	RGCGCY	2 cut(s)	617, 782
HaeIII	GGCC	9 cut(s)	113, 475, 687, 866, 1017, 1176, 1815, 2114, 2136
HapII	CCGG	2 cut(s)	1596, 1887
HhaI	GCGC	2 cut(s)	616, 781
Hin6I	GCGC	2 cut(s)	614, 779
HinP1I	GCGC	2 cut(s)	614, 779
HpaII	CCGG	2 cut(s)	1596, 1887
HphI	GGTGA	6 cut(s)	560, 660, 1151, 1486, 2077, 2282
Hpy166II	GTNNAC	6 cut(s)	99, 127, 883, 1039, 1447, 1603
Hpy188I	TCNGA	7 cut(s)	198, 389, 484, 1189, 1297, 1454, 2223
Hpy188III	TCNNGA	9 cut(s)	267, 407, 759, 872, 1542, 1665, 1727, 2248, 2284
Hpy8I	GTNNAC	6 cut(s)	99, 127, 883, 1039, 1447, 1603
Hpy99I	CGWCG	1 cut(s)	2065
HpyAV	CCTTC	5 cut(s)	494, 973, 973, 1805, 2218
HpyCH4IV	ACGT	1 cut(s)	940
HpyF10VI	GCNNNNNNNGC	7 cut(s)	550, 710, 785, 794, 1094, 1259, 1821
HpyF3I	CTNAG	7 cut(s)	312, 648, 793, 1000, 1264, 1481, 2220
HpySE526I	ACGT	1 cut(s)	940
HspAI	GCGC	2 cut(s)	614, 779
KpnI	GGTACC	1 cut(s)	1468
Kzo9I	GATC	6 cut(s)	160, 508, 934, 1486, 1864, 1882
LmnI	GCTCC	8 cut(s)	239, 363, 611, 776, 816, 1940, 2131, 2250
Lsp1109I	GCAGC	6 cut(s)	211, 262, 1079, 1109, 1600, 1754
LweI	GCATC	8 cut(s)	176, 818, 826, 1165, 1328, 1964, 1996, 2284
MaeI	CTAG	5 cut(s)	752, 1542, 1551, 1760, 1965
MaeII	ACGT	1 cut(s)	940
MaeIII	GTNAC	7 cut(s)	666, 1690, 1762, 1943, 2077, 2203, 2305
MalI	GATC	6 cut(s)	162, 510, 936, 1488, 1866, 1884
MboI	GATC	6 cut(s)	160, 508, 934, 1486, 1864, 1882
MboII	GAAGA	3 cut(s)	668, 2082, 2203
MfeI	CAATTG	4 cut(s)	306, 948, 954, 1470
MflI	RGATCY	2 cut(s)	508, 1882
MhlI	GDGCHC	3 cut(s)	129, 595, 1386
MlsI	TGGCCA	2 cut(s)	475, 687
MluNI	TGGCCA	2 cut(s)	475, 687
MlyI	GAGTC	3 cut(s)	488, 1117, 2296
MmeI	TCCRAC	1 cut(s)	1483
Mox20I	TGGCCA	2 cut(s)	475, 687
Mph1103I	ATGCAT	1 cut(s)	1409
MroXI	GAANNNNTTC	1 cut(s)	660
MscI	TGGCCA	2 cut(s)	475, 687
MslI	CAYNNNNRTG	1 cut(s)	1161
Msp20I	TGGCCA	2 cut(s)	475, 687
MspA1I	CMGCKG	2 cut(s)	713, 1004
MspI	CCGG	2 cut(s)	1596, 1887
MspR9I	CCNGG	6 cut(s)	321, 1239, 1596, 1887, 1888, 1940
MunI	CAATTG	4 cut(s)	306, 948, 954, 1470
Mva1269I	GAATGC	2 cut(s)	348, 400
MvaI	CCWGG	3 cut(s)	321, 1239, 1940
MvnI	CGCG	1 cut(s)	2060
MwoI	GCNNNNNNNGC	7 cut(s)	550, 710, 785, 794, 1094, 1259, 1821
NciI	CCSGG	3 cut(s)	1596, 1887, 1888
NcoI	CCATGG	3 cut(s)	688, 1049, 1162
NdeII	GATC	6 cut(s)	160, 508, 934, 1486, 1864, 1882
NlaIV	GGNNCC	7 cut(s)	510, 961, 1466, 1593, 1884, 2127, 2252
NmuCI	GTSAC	5 cut(s)	666, 1690, 1762, 2077, 2305
NsiI	ATGCAT	1 cut(s)	1409
NspI	RCATGY	1 cut(s)	1683
PaeR7I	CTCGAG	1 cut(s)	1385
PagI	TCATGA	1 cut(s)	871
PasI	CCCWGGG	1 cut(s)	1939
PceI	AGGCCT	1 cut(s)	1176
PcsI	WCGNNNNNNNCGW	1 cut(s)	2308
PctI	GAATGC	2 cut(s)	348, 400
PdmI	GAANNNNTTC	1 cut(s)	660
PfeI	GAWTC	9 cut(s)	290, 337, 390, 461, 623, 913, 1217, 2014, 2318
PflMI	CCANNNNNTGG	1 cut(s)	375
PfoI	TCCNGGA	1 cut(s)	319
PkrI	GCNGC	8 cut(s)	226, 277, 715, 1069, 1099, 1591, 1615, 1744
PleI	GAGTC	3 cut(s)	487, 1117, 2295
PpsI	GAGTC	3 cut(s)	487, 1117, 2295
Ppu21I	YACGTR	1 cut(s)	941
PpuMI	RGGWCCY	1 cut(s)	1547
Psp5II	RGGWCCY	1 cut(s)	1547
Psp6I	CCWGG	3 cut(s)	319, 1237, 1938
PspGI	CCWGG	3 cut(s)	319, 1237, 1938
PspN4I	GGNNCC	7 cut(s)	510, 961, 1466, 1593, 1884, 2127, 2252
PspPI	GGNCC	3 cut(s)	1016, 1547, 1814
PspPPI	RGGWCCY	1 cut(s)	1547
PspXI	VCTCGAGB	1 cut(s)	1385
PstI	CTGCAG	2 cut(s)	1744, 1912
PstNI	CAGNNNCTG	2 cut(s)	482, 716
PsuI	RGATCY	2 cut(s)	508, 1882
PvuII	CAGCTG	1 cut(s)	1004
RsaI	GTAC	4 cut(s)	1278, 1466, 1722, 2108
RsaNI	GTAC	4 cut(s)	1277, 1465, 1721, 2107
RseI	CAYNNNNRTG	1 cut(s)	1161
SatI	GCNGC	8 cut(s)	225, 276, 714, 1068, 1098, 1590, 1614, 1743
Sau3AI	GATC	6 cut(s)	160, 508, 934, 1486, 1864, 1882
Sau96I	GGNCC	3 cut(s)	1016, 1547, 1814
ScaI	AGTACT	1 cut(s)	1278
SchI	GAGTC	3 cut(s)	488, 1117, 2296
ScrFI	CCNGG	6 cut(s)	321, 1239, 1596, 1887, 1888, 1940
SduI	GDGCHC	3 cut(s)	129, 595, 1386
SfaNI	GCATC	8 cut(s)	176, 818, 826, 1165, 1328, 1964, 1996, 2284
SfcI	CTRYAG	7 cut(s)	244, 708, 900, 1200, 1209, 1740, 1908
Sfr274I	CTCGAG	1 cut(s)	1385
SinI	GGWCC	1 cut(s)	1547
SlaI	CTCGAG	1 cut(s)	1385
SmaI	CCCGGG	1 cut(s)	1888
SmiMI	CAYNNNNRTG	1 cut(s)	1161
SmlI	CTYRAG	2 cut(s)	294, 1385
SmoI	CTYRAG	2 cut(s)	294, 1385
SseBI	AGGCCT	1 cut(s)	1176
SsiI	CCGC	4 cut(s)	713, 1589, 1625, 2058
SspMI	CTAG	5 cut(s)	752, 1542, 1551, 1760, 1965
StuI	AGGCCT	1 cut(s)	1176
StyD4I	CCNGG	6 cut(s)	319, 1237, 1594, 1885, 1886, 1938
StyI	CCWWGG	4 cut(s)	688, 1049, 1162, 1224
TaiI	ACGT	1 cut(s)	943
TaqI	TCGA	3 cut(s)	108, 933, 1386
TatI	WGTACW	3 cut(s)	1276, 1720, 2106
TauI	GCSGC	2 cut(s)	716, 1592
TfiI	GAWTC	9 cut(s)	290, 337, 390, 461, 623, 913, 1217, 2014, 2318
TscAI	CASTG	6 cut(s)	838, 1048, 1066, 1489, 1662, 2082
TseFI	GTSAC	5 cut(s)	666, 1690, 1762, 2077, 2305
TseI	GCWGC	6 cut(s)	224, 275, 1067, 1097, 1613, 1742
Tsp45I	GTSAC	5 cut(s)	666, 1690, 1762, 2077, 2305
TspMI	CCCGGG	1 cut(s)	1886
TspRI	CASTG	6 cut(s)	838, 1048, 1066, 1489, 1662, 2082
Van91I	CCANNNNNTGG	1 cut(s)	375
VneI	GTGCAC	1 cut(s)	125
VpaK11BI	GGWCC	1 cut(s)	1547
XapI	RAATTY	4 cut(s)	192, 534, 1398, 2187
XbaI	TCTAGA	1 cut(s)	1541
XceI	RCATGY	1 cut(s)	1683
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1876
XhoI	CTCGAG	1 cut(s)	1385
XmaI	CCCGGG	1 cut(s)	1886
XmiI	GTMKAC	2 cut(s)	98, 1446
XmnI	GAANNNNTTC	1 cut(s)	660
XspI	CTAG	5 cut(s)	752, 1542, 1551, 1760, 1965
ZrmI	AGTACT	1 cut(s)	1278
Zsp2I	ATGCAT	1 cut(s)	1409

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.